Search

A coronary artery perforation is a rare, but potentially lethal, complication of percutaneous coronary intervention. We present a case of massive main vessel coronary perforation of the right coronary artery in a patient with acute ST segment elevation myocardial infarction, which was successfully treated with a second drug-eluting stent. This uncommon therapeutic approach was used to preserve flow to the large side branch. Early recognition, rapid balloon re-inflation at the perforation site and a “ping-pong” guiding technique allowed us to prepare the optimal strategy and to treat the perforation without developing cardiac tamponade.

Abstract COVID-19 infection remains a threat to the health systems of many countries. Potential success in the fight against the COVID-19 pandemic is the vaccination of high-risk groups, including patients with end-stage kidney disease (ESKD) and after solid organ transplantation (SOT). Immunosuppression in kidney transplant recipients can also reduce the immunogenicity of SARS-CoV-2 vaccines (varied by vaccine platform), available data suggest that they are efficacious in approximately 50–70%, compared to non-transplant situations. In this paper, we present a newly developed acute humoral and cellular rejection with acute allograft failure and need of hemodialysis 14 days after administration of the adenovirus vectored SARS-CoV-2 vaccine (AstraZeneca; CHADOx1, AZD1222). This occurred in a patient who previously had an asymptomatic COVID-19 infection. Case reports of acute allograft rejection after vaccination against SARS-CoV-2 can help stratify risk groups of patients who develop hyperimmune reactions. However, it is also possible that those with a previous mild primary COVID-19 infection may also develop acute allograft rejections upon COVID-19 re-infection.

Abstract Background The burden of Parkinson’s disease (PD) worsens with disease progression. However, the lack of objective and uniform disease classification challenges our understanding of the incremental burden in patients with advanced Parkinson’s disease (APD) and suboptimal medication control. The 5–2-1 criteria was proposed by clinical consensus to identify patients with advancing PD. Our objective was to evaluate the screening accuracy and incremental clinical burden, healthcare resource utilization (HCRU), and humanistic burden in PD patients meeting the 5–2-1 screening criteria. Methods Data were drawn from the Adelphi Parkinson’s Disease Specific Program (DSP™), a multi-country point-in-time survey (2017–2020). People with PD who were naive to device-aided therapy and on oral PD therapy were included. Patients meeting the 5–2-1 screening criteria had one or more of the three clinical indicators of APD: (i) ≥5 doses of oral levodopa/day, OR (ii) “off” symptoms for ≥2 h of waking day, OR (iii) ≥1 h of troublesome dyskinesia. Clinician assessment of PD stage was used as the reference in this study. Clinical screening accuracy of the 5–2-1 criteria was assessed using area under the curve and multivariable logistic regression models. Incremental clinical, HCRU, and humanistic burden were assessed by known-group comparisons between 5 and 2-1-positive and negative patients. Results From the analytic sample (n = 4714), 33% of patients met the 5–2-1 screening criteria. Among physician-classified APD patients, 78.6% were 5–2-1 positive. Concordance between clinician judgment and 5–2-1 screening criteria was > 75%. 5–2-1-positive patients were nearly 7-times more likely to be classified as APD by physician judgment. Compared with the 5–2-1-negative group, 5–2-1-positive patients had significantly higher clinical, HCRU, and humanistic burden across all measures. In particular, 5–2-1-positive patients had 3.8-times more falls, 3.6-times higher annual hospitalization rate, and 3.4-times greater dissatisfaction with PD treatment. 5–2-1-positive patients also had significantly lower quality of life and worse caregiver burden. Conclusions 5–2-1 criteria demonstrated potential as a screening tool for identifying people with APD with considerable clinical, humanistic, and HCRU burden. The 5–2-1 screening criteria is an objective and reliable tool that may aid the timely identification and treatment optimization of patients inadequately controlled on oral PD medications.

U ožujku 2014., nakon svrgavanja proruske vlade Viktora Janukoviča i uspostavljanja nove, prozapadne vlade u Ukrajini, Rusija je anektirala poluotok Krim koji je do tada bio u sastavu Ukrajine. Sam čin aneksije prikazivan je različito u europskim državama, a ovaj rad istražuje medijski prikaz u Hrvatskoj, Mađarskoj i Srbiji. Kao istraživačka metoda korištena je analiza sadržaja članaka i medijskih objava s relevantnih internetskih portala u istraživanim državama. Prema rezultatima analize sadržaja uočeno je kako se aneksija Krima u Hrvatskoj prikazuje sukladno prozapadnim politikama, u Mađarskoj se ističu određene domaće objektivnosti, dok je najviše proruskih stavova u izvješćivanju zamijećeno u Srbiji.

The paper presents an interesting opportunity to use a wide spread available energy source that has not yet been fully utilized. A new generator solution will be studied to produce electricity from oscillation energy. The solution will be useful for various purposes, especially for shipping, as well as freight road and rail transport. With this solution, it will be possible to reduce the usage of some amount of fossil fuels globally. Research includes two different theoretical assumptions. First approach involves the conversion of energy using Torus Type Generator (TTG) and second using Eccentric Type Generator (ETG). In TTG case, a new type of generator in which a magnetic cylinder moves freely in a hollow torus with electrical winding has been analysed. In the case of an ETG generator, a physical pendulum with a vertical axis of rotation has been analysed. Experimental and numerical methods were used for research. For the numerical analysis of 3D body motion the ANSYS Rigid Body Dynamics software was used.

Rasprava o moralnoj relevantnosti namjera danas se značajnim dijelom svodi na pitanje o tome jesu li namjere relevantne za moralnu dopustivost. Oni koji na to pitanje odgovaraju afirmativno, svoje stajalište opravdavaju povlačenjem distinkcije između namjernog činjenja štete i štete koja nastaje kao nenamjeravani, ali predvidljivi popratni učinak nekog djelovanja. Cilj ovog članka jest kritički preispitati navedeno stajalište – također poznato kao načelo dvostrukog učinka – te pružiti alternativno shvaćanje moralne relevantnosti namjera. Prema tom shvaćanju, temelj za moralnu pohvalu i osudu nije u izvršenoj radnji, već u namjeri s kojom je ta radnja izvršena.

Estimated glomerular filtration rate (eGFR) is one of the best-performing methods in evaluating kidney function. There are limited data regarding the estimated glomerular filtration rate in children and young adults with hemophilia. The aim of this study was to determine the difference between three commonly used estimated glomerular filtration rate equations in the pediatric population in a cohort of patients with hemophilia. Our prospective study included 36 pediatric patients with moderate or severe hemophilia. eGFR was calculated for each patient using the original creatinine-based “bedside Schwartz” equation, the cystatin C-based equation and the creatinine-cystatin C-based equation. The difference between the equations, calculated using the one-way repeated ANOVA test, was statistically significant (p

V članku je obravnavana zgodnja slovenščina, tj. južnoslovanski jezik v vzhodnoalpskem in zahodnopanonskem prostoru, ki se je v 11. in 12. stoletju razprostiral od Donave na severu do Jadrana na jugu in roba Panonske nižine na vzhodu. Prikazan je zemljepisni obseg slovanskega jezikovnega prostora v Vzhodnih Alpah, kakor se kaže predvsem v pisnih virih od konca 6. do vključno 12. stoletja. Na osnovi jezikoslovne analize v virih dokumentiranih zemljepisnih in osebnih imen so določene jezikovne lastnosti zgodnje slovenščine, in sicer v prvi vrsti v razmerju do tedaj zemljepisno stičnih slovanskih geolektov na severu (poznejši češčina in slovaščina) in jugu (poznejša čakavščina).

Drill bits with internal cooling capabilities are still not employed in stone machining practices within shop floor environments. Therefore, a conventional industrial drill bit used in stone machining was subject to a redesign wherein an axial cooling channel was machined throughout its body. A comparison was drawn between the standard drill bit without cooling capabilities and the redesigned drill bit, which used compressed air as a cooling medium. The experiment was performed by drilling three types of stone samples varying in hardness with nine combinations of cutting speed and feed rate. During the machining process, two types of process signals were continuously measured—namely, cutting forces and vibrations. Additionally, the cutting edges of the drill bits were inspected after a specific number of drilling cycles using a vision system. The primary objective of this study was to compare the cutting forces and tool wear dynamics achieved by those two drill bits. Furthermore, the usage of vibration signals in the classification of stone hardness during machining with an internally cooled drill bit was additionally analyzed. The results of this study unveiled improvement in minimizing cutting forces, vibrations, and the intensity of tool wear when utilizing an internally cooled drill bit. Even though the machining system generally exhibited lower vibrations, vibration signals again demonstrated commendable efficacy in classifying stone hardness.

Background and Objectives: An effective strategy for cardiopulmonary resuscitation should be based on tissue perfusion. Our primary aim was to determine the association between capillary lactate values and initial rhythm as well as the probability of the return of spontaneous circulation in out-of-hospital cardiac arrest patients. Materials and Methods: This prospective observational cohort study included all patients with non-traumatic out-of-hospital cardiac arrest, older than 18 years, resuscitated by a prehospital emergency medical team between April 2020 and June 2021. Capillary lactate samples were collected at the time of arrival and every 10 min after the first measurement until the time of the return of spontaneous circulation (ROSC) or, if ROSC was not achieved, at the time of declaring death on the scene. Results: In total, 83 patients were enrolled in the study. ROSC was achieved in 28 patients (33.7%), 21 were admitted to hospital (26.3%), and 6 (7.23%) of them were discharged from hospital. At discharge, all patients had Cerebral Performance Category Scale 1 or 2. Initial capillary lactate values were significantly higher in patients with a non-shockable rhythm compared to the group with a shockable rhythm (9.19 ± 4.6 versus 6.43 ± 3.81; p = 0.037). A significant difference also persisted in a second value taken 10 min after the initial value (10.03 ± 5,19 versus 5.18 ± 3.47; p = 0.019). Capillary lactate values were higher in the ROSC group and non-ROSC group at the time of restored circulation (11.10 ± 6.59 and 6.77 ± 4.23, respectively; p = 0.047). Conclusions: Capillary lactate values are significantly higher in patients with a non-shockable first rhythm in out-of-hospital cardiac arrest (OHCA). There is also a significantly different rise in capillary lactate levels in patients with ROSC.

Heart failure remains a major global burden regarding patients’ morbidity and mortality and health system organization, logistics, and costs. Despite continual advances in pharmacological and resynchronization device therapy, it is currently well accepted that heart transplantation and mechanical circulatory support represent a cornerstone in the management of advanced forms of this disease, with the latter becoming an increasingly accepted treatment modality due to the ongoing shortage of available donor hearts in an ever-increasing pool of patients. Mechanical circulatory support strategies have seen tremendous advances in recent years, especially in terms of pump technology improvements, indication for use, surgical techniques for device implantation, exchange and explantation, and postoperative patient management, but not in the field of treatment of critically ill patients and those undergoing cardiac arrest. This contemporary review aims to summarize the collected knowledge of this topic with an emphasis on complications in patients with left ventricular assist devices, their treatment, and establishing a clear-cut algorithm and the latest recommendations regarding out-of-hospital or emergency department management of cardiac arrest in this patient population.

Apple proliferation, caused by ‘Candidatus Phytoplasma mali’, is one of the most important economic threats in the field of apple production. Especially at a young age, infected trees can be affected by excessive bud proliferation and general decline. The fruit quality is also significantly reduced by this disease. To investigate treatment options, we applied a clarithromycin chemotherapy to infected in vitro cultures of ‘Golden Delicious’. With increasing concentrations of clarithromycin in the media, the phytoplasma load decreased rapidly after one month of treatment, but phytotoxicity led to a pronounced mortality at 40 mg/L, which was the highest dose used in our experiment. Out of 45 initial explants, we obtained one negative mericlone and two mericlones with a concentration of phytoplasma DNA at the detection limit of PCR. The culture propagated from the mericlone that tested negative remained phytoplasma-free after 18 months of subculturing. Our results suggest the applicability of macrolide antibiotics against phytoplasma infections in vitro; however, it might be challenging to find the threshold zone where the concentration is sufficient for pathogen elimination, but not lethal for the plant material of different cultivars.

Mountain bikes with electric assistance (e-bikes) have gained popularity recently by allowing riders to increase their pedaling power through an electric motor. This innovation has raised questions about how e-bikes compare to traditional mountain bikes regarding physical effort, speed, and physiological demands. By examining these factors, the study aims to compare and characterize differences in performance-related parameters when using an electric-assisted mountain bike compared to a conventional mountain bike on different types of terrain (uphill, downhill, flat section, technically demanding terrain) concerning power output, velocity, cardiorespiratory parameters, and energy expenditure. Six experienced mountain bikers (mean age: 44.6 ± 6.4 years, mean body height: 173.3 ± 5.6 cm, mean body weight: 70.6 ± 4.9 kg) cycled 4.5 km on varying off-road terrain at their own race pace, once with and once without electrical assistance, in randomized order. The results of the study indicate significantly faster (24.3 ± 1.85 to 17.2 ± 1.22 km/h (p < 0.001)) cycling on an electric-assisted mountain bike, which reduces cardiorespiratory parameters and metabolic effort as well as results in less demanding workload (138.5 ± 31.8 W) during the cycling with an electric-assisted mountain bike in comparison to a conventional mountain bike (217.5 ± 24.3 W (p < 0.001)). The results indicate significant differences especially when riding uphill. The performance advantage of an electrically assisted mountain bike diminishes compared to a conventional mountain bike on downhill, flat, or technically challenging terrain. The highlighted advantages of electric-assisted mountain bikes could represent a novel strategy for cycling in different terrains to optimize efficiency.

The oil content and fatty acid composition were determined in the seed of 19 poppy genotypes (both landraces and modern cultivars) grown in three-year field trials. The total oil content ranged from 34.56-44.76%. The oil content in white-seeded genotypes (40.73-44.76%) exceeded the oil content in blue-seeded genotypes (34.56-40.34%) and ocher-seeded genotypes (38.36-42.69%). Linoleic acid (71.41-74.02%), oleic acid (12.35-15.51%) and palmitic acid (8.95-10.29%) were the most abundant fatty acids in the evaluated seeds of poppy genotypes. A significant negative correlation (-0.7574**) was found between linoleic and oleic fatty acids. The sum of polyunsaturated (PUFA), monounsaturated (MUFA) and saturated (SFA) fatty acids ranged from 72.43-74.91%, 12.90-16.14% and 10.99-12.46% of the total fatty acids, respectively. Both the total oil content and the content of individual fatty acids were mainly affected by the crop year (weather conditions); however, the effect of genotype and year × genotype interaction was also significant. Due to the favourable composition of fatty acids, the evaluated poppy genotypes can be a good source of nutritionally valuable oil.

Reflection on the interpretation of late socialism.

Abstract Clinical pharmacists have not yet become an integral part of interdisciplinary ward rounds in most psychiatric hospitals across the European Union. This retrospective observational pre-post study examined the impact of clinical pharmacist recommendations in an interdisciplinary medical team during psychiatric hospital rounding. The study included all patients in a Slovenian psychiatric hospital who were hospitalized 2019–2020. The clinical pharmacist made 315 recommendations for a total of 224 participants (average age M = 59.4, median = 56). Psychiatrists accepted 295 (93.7%) of the recommendations. After the recommendations, the number of expressed and potential drug-related problems decreased in 166 (93.8%) and 129 (93.8%) interventions, respectively. Three months after discharge, 222 accepted recommendations were continued (70.5%). The most common recommendations were related to antipsychotics (19.4%, N = 61) followed by antidepressants (16.8%, N = 53). Including a clinical pharmacist in the interdisciplinary ward rounds at a psychiatric hospital reduced the number of expressed and potential drug-related problems with a very high recommendation acceptance rate. These results are the first in Central Europe to explore the benefits of including a clinical pharmacist in ward rounding.

Abstract Background Charcot-Marie-Tooth 1C (CMT1C) is a rare form of dominantly inherited CMT1 neuropathy caused by a mutated gene encoding lipopolysaccharide-induced tumour necrosis alpha factor (LITAF). Case presentation We report a 56-year-old patient with an atypical clinical phenotype of CMT1C, which started as progressive weakness of a single upper limb resembling acquired inflammatory neuropathy. Nerve conduction studies (NCS) and temporarily limited and partial effects of immunotherapy supported the diagnosis of inflammatory neuropathy. Significant progression of polyneuropathy, despite intensive long-lasting immunotherapy, together with repeatedly negative auxiliary investigations (CSF, MRI and antibodies) and genetic testing results finally led to the diagnosis of CMT1C neuropathy. Conclusions CMT1C should be added to the list of inherited neuropathies that need to be considered in suspected cases of inflammatory demyelinating neuropathy.

Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy (both unfractionated heparin and low-molecular-weight heparin). In our study, we examined a group of 122 patients with suspected HIT. The samples of all patients were analyzed in the first step using an immunoassay (ID-PaGIA Heparin/PF4, Hemos1L-Acustar HIT IgG, ZYMUTEST HIA Monostrip IgG) to detect the presence of antibodies against heparin–PF4 complexes (platelet factor 4). When the immunoassay was positive, the sample was subsequently analyzed for HIT with a functional flow cytometry assay, the HITAlert kit, the purpose of which was to demonstrate the ability of the antibodies present to activate platelets. A diagnosis of HIT can be made only after a positive functional test result. In this article, we present an overview of our practical experience with the use of the new functional method of analysis, HIT, with flow cytometry. In this work, we compared the mutual sensitivity of two functional tests, SRA and the flow cytometry HITAlert kit, in patients perceived as being at risk for HIT. This work aims to delineate the principle, procedure, advantages, pitfalls, and possibilities of the application of the functional test HITAlert using flow cytometry.

Background and Objectives: Despite advances in the treatment of heart diseases, the outcome of patients experiencing sudden cardiac arrest remains poor. The aim of our study was to determine the prehospital variables as predictors of survival outcomes in out-of-hospital cardiac arrest (OHCA) victims. Materials and Methods: This was a retrospective observational cohort study of OHCA cases. EMS protocols created in accordance with the Utstein style reporting for OHCA, first responder intervention reports, medical dispatch center dispatch protocols and hospital medical reports were all reviewed. Multivariate logistic regression was performed with the following variables: age, gender, witnessed status, location, bystander CPR, first rhythm, and etiology. Results: A total of 381 interventions with resuscitation attempts were analyzed. In more than half (55%) of them, bystander CPR was performed. Thirty percent of all patients achieved return of spontaneous circulation (ROSC), 22% of those achieved 30-day survival (7% of all OHCA victims), and 73% of those survived with Cerebral Performance Score 1 or 2. The logistic regression model of adjustment confirms that shockable initial rhythm was a predictor of ROSC [OR: 4.5 (95% CI: 2.5–8.1)] and 30-day survival [OR: 9.3 (95% CI: 2.9–29.2)]. Age was also associated (≤67 years) [OR: 3.9 (95% CI: 1.3–11.9)] with better survival. Conclusions: Elderly patients have a lower survival rate. The occurrence of bystander CPR in cardiac arrest remains alarmingly low. Shockable initial rhythm is associated with a better survival rate and neurological outcome compared with non-shockable rhythm.

This paper assesses the fault-detection capabilities of modern deep-learning models. It highlights that a naive deep-learning approach optimized for accuracy is unsuitable for learning fault-detection models from time-series data. Consequently, out-of-the-box deep-learning strategies may yield impressive accuracy results but are ill-equipped for real-world applications. The paper introduces a methodology for estimating fault-detection delays when no oracle information on fault occurrence time is available. Moreover, the paper presents a straightforward approach to implicitly achieve the objective of minimizing fault-detection delays. This approach involves using pseudo-multi-objective deep optimization with data windowing, which enables the utilization of standard deep-learning methods for fault detection and expanding their applicability. However, it does introduce an additional hyperparameter that needs careful tuning. The paper employs the Tennessee Eastman Process dataset as a case study to demonstrate its findings. The results effectively highlight the limitations of standard loss functions and emphasize the importance of incorporating fault-detection delays in evaluating and reporting performance. In our study, the pseudo-multi-objective optimization could reach a fault-detection accuracy of 95% in just a fifth of the time it takes the best naive approach to do so.

A three-dimensional motion capture system (MoCap) and the Garmin Running Dynamics Pod can be utilised to monitor a variety of dynamic parameters during running. The present investigation was designed to examine the validity of these two systems for determining ground contact times while running in place by comparing the values obtained with those provided by the bilateral force plate (gold standard). Eleven subjects completed three 20-s runs in place at self-selected rates, starting slowly, continuing at an intermediate pace, and finishing rapidly. The ground contact times obtained with both systems differed significantly from the gold standard at all three rates, as well as for all the rates combined (p < 0.001 in all cases), with the smallest mean bias at the fastest step rate for both (11.5 ± 14.4 ms for MoCap and −81.5 ± 18.4 ms for Garmin). This algorithm was developed for the determination of ground contact times during normal running and was adapted here for the assessment of running in place by the MoCap, which could be one explanation for its lack of validity. In conclusion, the wearables developed for monitoring normal running cannot be assumed to be suitable for determining ground contact times while running in place.

Apple hammerhead viroid (AHVd) was detected in the apple cultivar ‘Šampion’ and in mixed infection with Solanum nigrum ilarvirus 1 (SnIV-1) in the cultivars ‘Selena’ and ‘Jonagored Supra’, using a high-throughput sequencing method. Experiments were conducted to eliminate both pathogens in apples using meristem tip cultures in combination with the antivirotics ribavirin, rimantadine, and zidovudine. Elimination of both pathogens was verified by repeated RT-PCR and qRT-PCR assays after 7–11 months. Elimination of SnIV-1 from all cultivars was successful with each of the three antivirotics at concentrations of 20, 40, and 80 mg L−1. Elimination of AHVd was also achieved, although less effectively and only with ribavirin in the concentration range of 20–160 mg L−1.

Atherosclerosis is the primary process that underlies cardiovascular disease. The connection between LDL cholesterol and the formation of atherosclerotic plaques is established by solid evidence. PCSK9 inhibitors have proven to be a valuable and practical resource for lowering the LDL cholesterol of many patients in recent years. Their inhibitory effect on atherosclerosis progression seems to be driven not just by lipid metabolism modification but also by LDL-independent mechanisms. We review the effect of PCSK9 inhibitors on various mechanisms involving platelet activation, inflammation, endothelial dysfunction, and the resultant clot formation. The main effectors of PCSK9 activation of platelets are CD36 receptors, lipoprotein(a), oxidised LDL particles, tissue factor, and factor VIII. Many more molecules are under investigation, and this area of research is growing rapidly.

The present study was designed to determine the contribution of the cross-sectional area of the ski poles (Sp) to the total aerodynamic drag during alpine skiing. At three different wind speeds in a wind tunnel, 10 skiers assumed typical alpine skiing postures (high, middle, and tuck), and their frontal aerodynamic drag was assessed with a force plate and their cross-sectional area, along with that of their ski poles, determined by interactive image segmentation. The data collected were utilized to examine intra-subject variation in Sp, the effects of Sp on the coefficient of aerodynamic drag (Cd), and the product of Cd and total cross-sectional area (Cd∙S. The major findings were as follows: (i) Sp ranged from 0.0067 (tuck position) to 0.0262 m2 (middle position), contributing 2.2–4.8% of the total cross-sectional area, respectively; (ii) Sp was dependent on wind speed in the high and middle positions; (iii) intra-subject variations ranged from 0.0018 m2 (27.6%) in the tuck position to 0.0072 m2 (30.5%) in the high position; (iv) Sp exerted a likely effect on Cd and Cd∙S. The extensive intra- and inter-skier variability in Sp can account for as much as ~5% of the total frontal cross-sectional area and future investigations on how elite skiers optimize their positioning of the poles in a manner that reduces aerodynamic drag are warranted.

The exact incidence of cancer-associated venous thromboembolism (CA-VTE) in patients with oral and facial cancer (OFC) is not exactly known, and this risk is empirically considered to be low. However, this suggestion may result in disease underdiagnosis, prolong the initiation of adequate therapy, and consecutively increase CA-VTE-related morbidity and mortality. In addition, there might be specific clinical problems in the treatment of CA-VTE in patients with oral and facial cancer, such as swallowing difficulties, that might limit the possibilities of oral anticoagulation. Finally, there are limited data regarding the optimal treatment of CA-VTE in patients with oral and facial cancer, and this includes data on novel therapeutic strategies, including the use of direct oral anticoagulants. This article reviews current data on the optimal treatment strategy for CA-VTE in patients with OFC.

The paper presents a description and comparison of the undergraduate student teacher training and kindergarten teacher continuing education in the Montessori Method of Education encompassed in formal education in Slovakia and the Czech Republic. The theoretical part of the paper aims at depicting Montessori education, still an attractive pedagogical direction in both countries, in a thorough, unconventional and innovative way. Even though Montessori education has been rooted worldwide for centuries, in Slovakia and the Czech Republic it presents relatively new alternative education, considering that an integrated school system, introduced in these countries in 1948 and lasting almost to the end of20thcentury, did not allow the use of other alternatives in addition to the mainstream education. A description of (preschool) education in both countries and an analysis of kindergarten teacher's personality and his/her lesson plans are included in the theoretical part of the paper. The empirical part contains pedagogical research of qualitative design, conducted in Slovakia and the Czech Republic in order to identify and map the current state, level and possibilities of formal education of pre-service and in-service teachers in Slovak and Czech kindergartens.

Abstract The role of genetics in the causation of cerebral palsy has become the focus of many studies aiming to unravel the heterogeneous etiology behind this frequent neurodevelopmental disorder. A recent paper reported two unrelated children with a clinical diagnosis of cerebral palsy, who carried the same de novo c.1000G > A (p.Asp334Asn) variant in FBXO31, encoding a widely studied tumor suppressor not previously implicated in monogenic disease. We now identified a third individual with the recurrent FBXO31 de novo missense variant, featuring a spastic‐dystonic phenotype. Our data confirm a link between variant FBXO31 and an autosomal dominant neurodevelopmental disorder characterized by prominent motor dysfunction.

BackgroundThe aim of this study was to analyze the relation between the hepatic fibrosis markers, liver morphology and hemodynamics assessed by magnetic resonance imaging (MRI) after total cavopulmonary connection (TCPC).Materials and methodsAdult patients after TCPC performed in childhood between 1993 and 2003 are the subjects of this observational study. The follow-up protocol consisted of clinical and echocardiographic examination, liver elastography, cardiopulmonary exercise test, MRI hemodynamics and liver morphology assessment and direct enhanced liver fibrosis (ELF) test.ResultsThe cohort consisted of 39 patients (46% female) with a median age at study 26 (IQR 23–28) years and interval from TCPC 21 (IQR 20–23) years. There was no correlation between ELF test and any MRI variables, but procollagen III amino-terminal peptide (PIIINP), a single component of ELF test, correlated significantly with ventricular end-diastolic volume (r = 0.33; p = 0.042) and inferior vena cava flow (r = 0.47; p = 0.003). Fifteen (38%) patients with liver nodules had compared to other 24 patients higher end-diastolic volume (ml/m2) 102.8 ± 20.0 vs. 88.2 ± 17.7; p = 0.023, respectively. PIIINP correlated significantly with inferior vena cava flow (r = 0.56; p = 0.030) and with end-diastolic volume (r = 0.53; p = 0.043), but only in patients with liver nodules.ConclusionGradual progression of liver fibrosis, particularly hepatic arterialization caused by liver nodules formation, increases inferior vena cava flow and subsequent ventricular volume overload may further compromise single ventricle functional reserve in adult patients after TCPC.

The main goal was to determine anaerobic and aerobic improvement of young soccer players after six-week high volume (HVT) or small sided games (SSG) training intervention. One hundred and one highly trained youth soccer players (16.2 ± 1.3 years) were divided into SSG (n = 51) and HVT groups (n = 50) and according to age into an under sixteen subgroup (U16), under seventeen subgroup (U17), and under nineteen subgroup (U19). The performance was assessed by Yo-Yo intermittent test, Repeated sprint ability test (RSA), and K-test before and after both training interventions. For U16 the SSG group recorded significant improvements in the K-test (0.64 ± 0.56 s; p = .04) and RSA (0.15 ± 0.43 s; p = .01). For U19 the SSG group recorded the same improvements, in the K-test (0.43 ± 0.57 s; p = .007), RSA (0.21 ± 0.22 s; p = .048), and Yo-Yo test (127.25 ± 17.87; p = .049). HVT improved aerobic performance when the Yo-Yo test was significantly better after intervention at U17 (199.00 ± 111.83 m; p = .030), U19 (88.40 ± 66.38 m; p = .049). In total, the HVT group spent 621 min (56.45 ± 5.01 min) of aerobic training and the Small sided game group spent 291 min (26.45 ± 8.61 min) of small sided games focused on aerobic performance. This study showed that both SSG and HVT training interventions were effective for aerobic improvement for the U19 category, but not for younger players. SSG was identified to be more appropriate to fitness development of soccer players.

This study aimed to analyze blood pressure (BP) patterns, assess blood pressure variability (BPV), and its possible determinants in children with essential hypertension. The study group included 132 children with essential hypertension without antihypertensive therapy. Anthropometric and laboratory parameters were evaluated, office and ambulatory BP were measured. BPV was defined as the standard deviation of BP for the day and nighttime periods. In addition to classical statistical analysis, an unsupervised machine learning approach using the expected maximization algorithm was implemented to find groups of patients with similar characteristics. No differences in BPV were observed between sexes; however, boys had higher levels of creatinine, serum glucose, and uric acid despite similar body mass index values. There was a significant correlation between the Zscore for body mass index and daytime systolic BPV (r=0.19, p

Aim: In this case-control study we wanted to compare the perinatal outcome of naturally conceived newborns to those born after in vitro fertilization (IVF). Methods: At the University Hospital Centre Osijek, in the period from January 2014 to December 2016, we analysed 120 in vitro fertilization pregnancies and compared to 120 natural conception pregnancies. Characteristics of mothers, birth method, perinatal outcome, and vital characteristics of newborns were analysed. Results: IVF-ET pregnancies included 70.0% singleton pregnancies, 28.3% twin and 1.7% triplet pregnancies, while all naturally conceived pregnancies were singletons. When pregnancies were compared between IVF-ET and naturally conceived groups, the following characteristics of mothers were established: age 34.83 (5.8):30.39 (3.9); previous pregnancy 13.3%:56.7%; complications in pregnancy 50.0%:25.0%; Caesarean section (CS) 69.2%:35.8%. All of these were statistically significant (p < 0.001). Comparison of newborns between those two groups established the following: prematurity 39.5% vs. 12.7%; lowest BW 2,114 vs. 3,000 grams; lowest GA 22.29 vs. 28.71 weeks. In the IVF-ET group, 5.7% of newborns had a gestational age of 22-25 weeks, but there were no newborns of that gestational age among the naturally conceived newborns. These were also statistically significant differences (p < 0.001). Apgar score was 10 for both groups, but the difference was in the interquartile range, the values of which were lower in the IVF-ET group. Conclusion: In vitro fertilization pregnancies are high risk due to the characteristics of both the mother and the infant and, as such, require special attention and care.

V prispevku je makedonščina opredeljena geneo-, tipo- in sociolingvistično. S stališča genealoškega jezikoslovja je s pomočjo relativne kronologije in zemljepisne razširjenosti relevantnih jezikovnih sprememb prikazano oblikovanje makedonščine in bolgarščine kot geolektov znotraj vzhodne južne slovanščine. Z gledišča tipološkega jezikoslovja je umeščena v kontekst balkanske jezikovne zveze. Sociolingvistični pogled pa prikaže proces standardizacije in pravni položaj sodobnega makedonskega knjižnega/standardnega jezika kot sociolekta.

The total antioxidant activity (TAA), total contents of polyphenols (TPC), phenolic acids (TPA) and tocols (TTC) were determined in the grain of 5 oat cultivars of Czech origin grown under organic and conventional cropping systems in two-year experiments (2018 and 2019). The TPC ranged from 772.9 mg/kg DM (dry matter) (hulled oat cv. Seldon) to 890.6 mg/kg (naked oat cv. Patrik); the TPA from 261.6 mg/kg (cv. Seldon) to 479.0 mg/kg (cv. Patrik); the TTC from 110.9 mg/kg (hulled oat cv. Korok) to 126.5 mg/kg (cv. Seldon). The TAA ranged from 427.1 mg/kg (cv. Korok) to 474.9 mg/kg (cv. Seldon). Besides the effect of the cultivar, the TAA and antioxidant contents were significantly affected also by year (weather conditions); higher values were observed in the drier and warmer the year 2019. The effect of the cropping system was statistically insignificant.

Aim: The study aimed to examine LDL cholesterol, triglyceride and urate levels in patients of both sexes with type 2 diabetes mellitus (DM2T) in family medicine offices and to examine whether there is a difference in these parameters between obese patients and patients with normal body weight with DM2T, and between patients with and without manifest cardiovascular diseases. Participants and methods: The study was organized as a cross-sectional study. It included 136 participants of both sexes diagnosed with DM2T, who were divided into groups of patients with or without adiposity and into groups of patients with or without experience of cardiovascular event. General and demographic data were collected, as well as data on experiencing cardiovascular events and levels of LDL cholesterol, triglycerides and urates. Results: The average LDL cholesterol level was 2.93 mmol/L, the average triglyceride level was 1.65 mmol/L and the average urate level was 326.36 µmol/L. Only 12.5% of participants reached target LDL cholesterol levels, while levels of triglycerides and urates were within recommended limits. 24.3% of participants had experienced cardiovascular events and 39.7% of participants were obese. There was no significant difference in levels of LDL cholesterol, triglycerides and urates in participants who had experienced a cardiovascular event and those who had not. There was a significantly higher concentration of triglycerides in obese patients than in patients with normal body weight (p = 0.005). Conclusion: In addition to regulation of glycaemia in patients with DM2T, statin doses should be increased in order to reach the target levels of LDL cholesterol. When it comes to obese patients, education courses on physical activity and diet should be conducted more often and, if necessary, fibrates should be included in therapy in order to reduce additional cardiovascular risks.

Abstract Background There are gaps in our neurobiological understanding of functional movement disorder (FMD). Objectives We investigated gray matter volumetric profiles in FMD, and related findings to resting‐state functional connectivity (rsFC) profiles using Human Connectome Project data. Methods Volumetric differences between 53 FMD patients and 50 controls were examined, as well as relationships between individual differences in FMD symptom severity and volumetric profiles. Atrophy network mapping was also used to probe whether FMD‐related structural alterations preferentially impacted brain areas with dense rsFC. Results Compared to controls without neurological comorbidities (albeit with mild depression and anxiety as a group), the FMD cohort did not show any volumetric differences. Across patients with FMD, individual differences in symptom severity negatively correlated with right supramarginal and bilateral superior temporal gyri volumes. These findings remained significant adjusting for FMD subtype or antidepressant use, but did not remain statistically significant adjusting for depression and anxiety scores. Symptom severity‐related structural alterations mapped onto regions with dense rsFC—identifying several disease epicenters in default mode, ventral attention, and salience networks. Conclusions This study supports that FMD is a multinetwork disorder with an important role for the temporoparietal junction and its related connectivity in the pathophysiology of this condition. More research is needed to explore the intersection of functional neurological symptoms and mood.

V članku so obravnavani slovanski števniki od 1 do 10 v pred kratkim odkritem latinskem rokopisu iz Heiligenkreuza iz 12. stoletja (Cod. 250), ki sta jih prva objavila in interpretirala Tadeusz Lewaszkiewicz in Wiesław Wydra (2021). Preko natančne zgodovinskoglasoslovne analize števnikov, katerih zapis je bil najprej interpretiran grafično in glasoslovno, je vzpostavljena osnova za genealoško jezikoslovno določitev slovanskega jezika, v katerem so bili zapisani. Ta je postavljen v kontekst tedanjega slovanskega jezika v vzhodnoalpsko-zahodnopanonskem prostoru ter le-temu zemljepisno stičnih slovanskih geolektov severno in južno od tega.

Courses on basic life support (BLS) and automated external defibrillator (AED) in schools lead to increase in knowledge but its retention is less well explored. We aimed to explore the long-term retention of knowledge and practical skills among schoolchildren after a BLS and AED course to be able to tailor future courses accordingly. Study was conducted in 3 parts and included 823 seventh and ninth graders from different elementary schools in Maribor, Slovenia. In Study 1 (n=611) we assessed students' baseline knowledge and immediate knowledge gain after our BLS and AED course with a validated questionnaire; in Study 2 (n=116) we assessed retention of gained knowledge and skills after 5 months with a modified Cardiff test and Little Anne QCPR manikin; in Study 3 (n=96) we assessed retention of knowledge 2 years after the course. Mean differences in knowledge before and after the course in Study 1 and between studies were analyzed using paired t-tests and independent t-tests. Differences between individual question scores at different time points were compared using Mann – Whitney U test. A two-sided P

Systemic infections with phytoplasmas and viruses threaten the production of healthy plant material under the fruit species certification system. We tested the possibility of sanitation using in vitro culture and cryotherapy. The starting material of the cultivars Golden Delicious (clones A and B), Virginia Crab, and Panenské zlepšené was taken from in vivo plants that tested positive for apple proliferation phytoplasma. The Táborita cultivar was obtained from already established in vitro cultures that had tested positive for apple proliferation phytoplasma, apple mosaic virus, and apple chlorotic leaf spot virus. Cultivars Golden Delicious A, Virginia Crab, and Panenské zlepšené were sanitated from the phytoplasma in the first step, i.e., by sterilization and a subsequent transfer to in vitro conditions. Golden Delicious B remained infected with the phytoplasma, and both viruses, after the in vitro culture phase and together with Táborita, were subjected to cryotherapy by vitrification. In Golden Delicious B, three out of thirteen initial shoot tips regenerated after a liquid nitrogen treatment. Four mericlones were regenerated from 10 initial cryopreserved shoot tips of Táborita. None of the three pathogens were detected by PCR in the regenerated Golden Delicious B mericlones. On the contrary, in the case of Táborita, infection with all the pathogens was detected after regeneration. The results obtained indicate the potential applicability of in vitro cultivation techniques or, if necessary, subsequent cryopreservation as a method for sanitizing against systemic microbial contamination. However, further research on the relationship between pathogens and specific genotypes is needed.

Bulk multiferroic ceramics have been extensively studied due to their great potential for magneto-electric coupling applications such as low-power and multifunctional nano-electronic devices. In most of these studies the macroscopic magnetic performance was investigated, while the magnetic response on the micro- and nano-scale was not examined in detail. Local magnetic phenomena can be studied using magnetic force microscopy (MFM), a technique derived from atomic force microscopy. MFM measures the magnetic force between the magnetised tip and the magnetic sample. It is one of the most used methods to characterise the structure of ferromagnetic domains, because the sample preparation is simple, non-destructive and provides a relatively high-resolution image. In this review paper we focus on the MFM analyses of bulk multiferroic ceramics. The core of the article is divided into four sections: the introduction, the preparation of samples prior to MFM examination, the reviews of MFM analyses performed on bulk multiferroic ceramics with and without external magnetic fields, and finally the conclusions and an outlook for the future.

As the microelectronic industry develops, components that can perform several different tasks receive increasingly more attention, resulting in multifunctional materials being highly sought after. Al2O3 is widely present in electronic applications as a protective coating or as an electrical and thermal insulator due to its mechanical and thermal stabilities and chemical inertness. Al2O3 is also an important dielectric material, with high resistivity and stable permittivity over a wide range of temperatures and electric fields, but its modest permittivity necessitates large effective areas or extremely thin layers when a large capacitance is desired. Composites consisting of discrete conducting phases within an insulating matrix can produce large capacitance via Maxwell–Wagner polarization. In this work, Al2O3/Al composite thick films with different volume ratios of Al were prepared using the aerosol deposition method. A relative dielectric permittivity (εr′) of 800 at 1 MHz was achieved at 27 vol% of Al, a sixty-sevenfold enhancement compared to Al2O3. On the other hand, dielectric losses, tan(δ), at 1 MHz increased from 0.01 for Al2O3 up to 0.58 for the composite with 27 vol% of Al. A finite-element model of the composites was implemented, supporting the nonlinear electrical behavior of the composites as function of vol% of Al. Our results show novel possibilities for the applications of Al2O3-based materials in the microelectronic industry, especially for temperature-sensitive applications, for which the integration strengths of aerosol deposition are valuable.