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We consider a system of interacting bosons in one dimension at a two-body resonance. This system, which is weakly interacting, is known to give rise to effective three-particle interactions, whose dynamics is similar to that of a two-dimensional Bose gas with two-body interactions, and exhibits an identical scale anomaly. We consider the experimentally relevant scenario of a harmonically trapped system. We solve the three-body problem exactly and evaluate the shifts in the frequency of the lowest compressional mode with respect to the dipole mode, and find that the effect of the anomaly is to increase the mode's frequency. We also consider the weak-coupling regime of the trapped many-boson problem and find, within the local density approximation, that the frequency of the lowest compressional mode is also shifted upwards in this limit. Moreover, the anomalous frequency shifts are enhanced by the higher particle number to values that should be observable experimentally., 8 pages, 2 figures

I consider non-relativistic bosons interacting via pairwise potentials with infinite scattering length and supporting no two-body bound states. To lowest order in effective field theory, these conditions lead to non-interacting bosons, since the coupling constant of the Lieb-Liniger model vanishes identically in this limit. Since any realistic pairwise interaction is not a mere delta function, the non-interacting picture is an idealisation indicating that the effect of interactions is weaker than in the case of off-resonant potentials. I show that the leading order correction to the ground state energy for more than two bosons is accurately described by the lowest order three-body force in effective field theory that arises due to the off-shell structure of the two-body interaction. For natural two-body interactions with a short-distance repulsive core and an attractive tail, the emergent three-body interaction is repulsive and, therefore, three bosons do not form any bound states. This situation is analogous to the two-dimensional repulsive Bose gas, when treated using the lowest-order contact interaction, where the scattering amplitude exhibits an unphysical Landau pole. The avoidance of this state in the three-boson problem proceeds in a way that parallels the two-dimensional case. These results pave the way for the experimental realisation of one-dimensional Bose gases with pure three-body interactions using ultracold atomic gases., 9 pages, 2 figures, accepted version. Added detailed discussion of the Faddeev equations

This paper aims to synthesize hybrid materials with high pollutant-uptake capacity and low costbased based on Luffa cylindrica (L.C) and different percentage of Zn2+ in the presence and absence of alternating current (AC). Physico-chemical, morphological and structural characterizations of the hybrid materials were performed by Boehm method, point zero charge (pHpzc), infrared characterizations (IR), scanning electron microscopy (SEM), energy&ndash, dispersive spectroscopyand and X-ray photoelectron spectroscopy. The efficiency of the designed hybrid materials was optimized based on their performance in water depollution. Methylene blue (MB) and industrial textile wastewater were the investigated pollutants models. IR characterizations confirmed the fixation of Zn2+ onto the L.C by the creation of Zn-OH, Zn-O and Zn-O-C bonds. Boehm titration showed that the fixation of Zn2+ onto L.C is accompanied by an increase of the basic functions of its surface and subsequently an increase in the pHpzc. SEM results confirmed the fixation of Zn2+ onto the L.C coupling AC with biosorption showed an increase in the adsorbed amount of MB and speed when adding the 4% of Zn2+ compared to the pure L.C the Qm shifted from 3.22 to 9.84 and 8.81 mg/g, respectively, for hybrid materials synthesized under AC, in absence of AC and pure L.C.

Finding effective treatment options for patients with brain metastasis remains an unmet need. Given the limitations imposed by the blood-brain-barrier for systemic approaches, radiotherapy offers a superior ability to access the brain. While whole-brain-radiation-therapy (WBRT) historically has been the gold standard in the management of brain metastasis patients, clinical practice rapidly adapted the frequent use of stereotactic radiosurgery (SRS), given the high incidence of neurotoxicity when giving WBRT. However, WBRT continuous to be an important treatment option, since many patients present with numerous multifocal lesions, high risk of SRS-associated radionecrosis, bad performance scores or probable distant intracranial recurrence, rendering them ineligible for SRS. Unfortunately, overall survival of patients remains unaffected by radiotherapy. In spite of this clinical data, the underlying molecular mechanisms that allow metastatic cells to resist radiotherapy in the brain is unknown. We have applied WBRT to experimental brain metastasis from lung and breast adenocarcinoma and validated their resistance in vivo. An unbiased search to identify potential mediators of resistance identified the S100A9-RAGE-NFkB-JunB pathway. Targeting this pathway genetically reverts the resistance to radiotherapy and increases therapeutic benefits in vivo. In two independent cohorts of brain metastasis from lung and breast adenocarcinoma patients, levels of S100A9 correlate with the response to radiotherapy, offering a novel approach to stratify patients according to their expected benefit. As a proof of concept, we also detected S100A9 in serum and CSF samples from brain metastasis patients, complementing our biomarker strategy with a less invasive approach. Furthermore, we have validated the use of a blood-brain-barrier permeable RAGE inhibitor to restore radio-sensitivity in experimental brain metastasis models in vivo and in patient-derived organotypic cultures of radio-resistant brain metastasis ex vivo. In conclusion, we identified S100A9 as a major mediator of radio-resistance in brain metastasis and offer the molecular framework to personalize radiotherapy by exploiting it as a biomarker and as a therapeutic target, thus maximizing the benefits for the patient. Citation Format: Lauritz Miarka, Catia Monteiro, Celine Dalmasso, Natalia Yebra, Coral Fustero-Torre, Aisling Hegarty, Stephen Keelan, Eduardo Caleiras, Vareslija Damir, Leonie Young, Riccardo Soffietti, Juan M. Sepúlveda, Angel Pérez, Aurelio Lain, Aurore Siegfied, Elizabeth Cohen-Jonathan Moyal, Manuel Valiente. S100A9 mediates radiation resistance of brain metastasis [abstract]. In: Proceedings of the AACR Virtual Special Conference on Radiation Science and Medicine; 2021 Mar 2-3. Philadelphia (PA): AACR; Clin Cancer Res 2021;27(8_Suppl):Abstract nr PO-068.

Lifestyle, especially diet, is a prominent risk factor that affects the formation of calcium oxalate stones. Urinary oxalate excretion is directly related to the amount of oral intake and intestinal absorption rate of oxalate. This work evaluated the possibility of increasing oxalate ingestion, which could lead to secondary hyperoxaluria, associated with the intake of herbal remedies and dietary supplements containing plant extracts. A wide variety of 17 commercially available drugs and dietary supplements were analyzed using ion chromatography. The results showed remarkable differences in oxalate contents of the extracts. Total oxalate concentrations ranged from 0.03 to 2.2 mg/g in solid samples and from 0.005 to 0.073 mg/mL in liquid samples. The selected herbal remedies and dietary supplements containing plant extracts represent only a low risk for calcium oxalate stone formers, if the recommended daily dose is not exceeded.

Mesoporous Hierarchical Alumina Microspheres (HAM) with high efficiency for fluoride removal have been synthesized and characterized. Two types of HAM, differing mostly in crystallinity, surface area and pore size have been obtained. Fluoride adsorption studies have been carried out by means of potentiometry and Isothermal Titration Calorimetry (ITC). The latter method has been applied for the first time to obtain direct determination of the adsorption enthalpy (ΔHads) of F− ion on HAM. The kinetics of the reaction revealed a two-step process for fluoride adsorption on the adsorbent material. The ΔHads values obtained are clearly negative for the different samples investigated. Experimental adsorption data are well fitted by a Langmuir isotherm. The adsorption constant obtained for type A is 1 order of magnitude higher than for type B, showing that the synthetic protocol has a remarkable effect on this parameter. The highest defluoridation capacity reaches 26 mmol g−1 after 1 hour of equilibration for the amorphous HAM, which is higher than for other adsorbents reported in the literature.

Tooth whitening materials have not undergone relevant advances in the last years. Current materials base their action on the oxidant activity of peroxides, which present the disadvantage of requiring long application times, along with unpleasant side effects of dental hypersensitivity (e.g. sharp pain). In this work, a novel tooth whitening formulation based on the encapsulation of a reducing agent (sodium metabisulfite) in liposomes is developed. An experimental design was applied to optimize the formulation in terms of whitening action and safety, using bovine teeth as in vitro model. Results were obtained by colorimetry, profilometry and nanoindentation techniques. The comparison with standard whitening treatments showed a similar whitening action of the optimized formulation but in remarkable shorter application times. Moreover, teeth roughness values obtained with the presented formulation conformed with ISO 28399. As mechanism of action, results obtained from fluorescent confocal microscopy showed the liposomal formulation to form a layer surrounding the enamel surface, enhancing the treatment efficacy in terms of diffusion of the protected reductant towards the enamel. The better efficiency of this formulation encourages its use as an alternative to current oxidative treatments.

The diagnosis of brain metastasis involves high morbidity and mortality and remains an unmet clinical need in spite of being the most common tumor in the brain. Exclusion of these cancer patients from clinical trials is a major cause of their limited therapeutic options. In this study, we report a novel drug-screening platform (METPlatform) based on organotypic cultures which allows identifying effective anti-metastasis agents in the presence of the organ microenvironment. We have applied this approach to clinically relevant stages of brain metastasis using both experimental models and human tumor tissue (by performing patient-derived organotypic cultures). We identified heat shock protein 90 (HSP90) as a promising therapeutic target for brain metastasis. Debio-0932, a blood-brain barrier permeable HSP90 inhibitor, shows high potency against mouse and human brain metastases from melanoma, lung and breast adenocarcinoma with distinct oncogenomic profiles at clinically relevant stages of the disease, including a novel model of local relapse after neurosurgery. Furthermore, we have also used METPlatform to perform unbiased proteomics of brain metastases in situ. By applying this analysis to brain metastases treated with the chaperone inhibitor, we uncovered non-canonical clients of HSP90 as potential novel mediators of brain metastasis and actionable mechanisms of resistance driven by autophagy. Combined therapy using HSP90 and autophagy inhibitors showed synergistic effects compared to sublethal concentrations of each monotherapy, demonstrating the potential of METPlatform to design and test rationale combination therapies to target metastasis more effectively. In conclusion, our work validates METPlatform as a potent resource for metastasis research integrating drug-screening and unbiased omic approaches that is fully compatible with human samples and questions the rationale of excluding patients with brain metastasis from clinical trials. We envision that METPlatform will be established as a clinically relevant strategy to personalize the management of metastatic disease in the brain and elsewhere.

The periodic application of copper-based fungicides (Bourdeaux mixture) to vineyards of the Mediterranean region has generated an important pollution source that in some cases requires a quick intervention due to the high bioavailable copper content measured. Despite some vineyards were abandoned 40 years ago, noticeable amounts of Cu and other man-related metals are still nowadays detected in soils. In the present work, the development of a mobility test for the available heavy metal (Cu, Pb, Zn and As) content in soil has been performed using portable X-ray fluorescence (FP-XRF) combined with single leaching test, and was applied to a calcareous soil of a former vineyard area in Catalonia (NE Spain). The combined methodology has provided useful information for fast and detailed risk assessment, in terms of mobility and bioavailability of metals. The anthropogenic contribution was evaluated by means of the Concentration Enrichment Ratios (CER) in soil. The results reflect a clear anthropogenic contribution for Cu, a partial anthropogenic contribution for Pb proceeding from an external pollution source, and a non-significant external contribution for As and Zn. The topsoil concentration ranges for Cu (70–128 mg kg−1) were found to be above the background level and several samples above the regional governmental limits (Generic Reference Levels or GRL values) for soil ecosystem protection and for human health (90 mg kg−1). The present study reveals that the use of FP-XRF equipment constitute a highly valid option for quick decision making during the field location, characterization and quantitative elemental analysis of soil samples for screening of potential pollutants such as heavy metals.

This paper presents a fast and automatic flow-based method to extract 131I from biological samples and hospital waste, previous to liquid scintillation detection. 131I is a radionuclide extensively used in Nuclear Medicine due to their beta and gamma disintegrations, whereby hospitals have to manage the associated waste generation. The automatic developed system is based on Lab-On-Valve (LOV) flow-technique exploiting Cl-resin (135 mg per extraction). This methodology allows performing sample extractions and measurements on the same day, since the extraction frequency takes 1.4–4 h−1, depending on the analysed sample volume, plus up to 2 h of measurement for each vial. 131I is retained as iodine ion and eluted with sodium sulphide 0.2 mol L−1. The maximum sample volume that can be preconcentrated is 20 mL, reaching an extraction efficiency of 85 ± 5%. The minimum detectable activity (MDA) is 0.05 Bq, showing a precision of 7% RSD (n = 5). Both, biological samples (urine and saliva) and hospital waste samples can be satisfactorily analysed by the proposed system, obtaining recoveries between 90 and 110%. The developed method is then suitable to implement in hospitals, improving the surveillance of the 131I environmental release.

We present experimental evidence of photon droplets in an attractive (focusing) nonlocal nonlinear medium. Photon droplets are self-bound, finite-sized states of light that are robust to size and shape perturbations due to a balance of competing attractive and repulsive forces. It has recently been shown theoretically, via a multipole expansion of the nonlocal nonlinearity, that the self-bound state arises due to competition between the s-wave and d-wave nonlinear terms, together with diffraction. The theoretical photon droplet framework encompasses both a soliton-like stationary ground state and the non-soliton-like dynamics that ensue when the system is displaced from equilibrium, i.e. driven into an excited state. We present numerics and experiments supporting the existence of these photon droplet states and measurements of the dynamical evolution of the photon droplet orbital angular momentum., 6 pages (inc. supplemental), 7 figures

Metastatic seeding by disseminated cancer cells principally occurs in perivascular niches. Here, we show that mechanotransduction signaling triggered by the pericyte-like spreading of disseminated cancer cells on host tissue capillaries is critical for metastatic colonization. Disseminated cancer cells employ cell adhesion molecule L1 (L1CAM) to spread on capillaries and activate the mechanotransduction effectors Yes-associated protein (YAP) and myocardin-related transcription factor (MRTF). This L1CAM-mediated spreading is robust enough to displace resident pericytes, which also use L1CAM for perivascular spreading. L1CAM activates YAP by engaging β1 integrin and integrin linked kinase (ILK). L1CAM-YAP signaling enables the outgrowth of metastasis-initiating cells both immediately upon their infiltration of target organs and after they exit from a period of dormancy. Our results identify an important step in the initiation of metastatic colonization, define its molecular constituents, and provide an explanation for the widespread association of L1CAM with metastatic relapse in the clinic.

We show that the realisation of synthetic magnetic fields via light-matter coupling in the Lambda-scheme implements a natural geometrical construction of magnetic fields, namely as the pullback of the area element of the sphere to Euclidean space via certain maps. For suitable maps, this construction generates linked and knotted magnetic fields, and the synthetic realisation amounts to the identification of the map with the ratio of two Rabi frequencies which represent the coupling of the internal energy levels of an ultracold atom. We consider examples of maps which can be physically realised in terms of Rabi frequencies and which lead to linked and knotted synthetic magnetic fields acting on the neutral atomic gas. We also show that the ground state of the Bose-Einstein condensate may inherit topological properties of the synthetic gauge field, with linked and knotted vortex lines appearing in some cases., 8 pages, 4 figures, supplementary videos attached. Comments welcome

Finite topologically non-trivial systems are often characterised by the presence of bound states at their physical edges. These topological edge modes can be distinguished from usual Shockley waves energetically, as their energies remain finite and in-gap. On a clean 1D or reducible 2D model, in either the commensurate or semi-infinite case, the edge modes can be obtained analytically, as shown in [PRL 71, 3697 (1993)] and [PRA 89, 023619 (2014)]. We put forward a method for obtaining the spectrum and wave functions of topological edge modes for arbitrary finite lattices, including the incommensurate case. A small number of parameters are easily determined numerically, with the form of the eigenstates remaining fully analytical. We also obtain the bulk modes in the finite system analytically and their eigenenergies, which lie within the infinite-size limit continuum. Our method is general and can be easily applied to obtain the properties of non-topological models and/or extended to include impurities. As an example, we consider the case of an impurity located next to one edge of a 1D system, equivalent to a softened boundary in a separable 2D model. We show that a localised impurity can have a drastic effect on the edge modes of the system. Using the periodic Harper and Hofstadter models to illustrate our method, we find that, on increasing the impurity strength, edge states can enter or exit the continuum, and a trivial Shockley state bound to the impurity may appear. The fate of the topological edge modes in the presence of impurities can be addressed by quenching the impurity strength. We find that at certain critical impurity strengths, the transition probability for a particle initially prepared in an edge mode to decay into the bulk exhibits discontinuities that mark the entry and exit points of edge modes from and into the bulk spectrum., 13 pages, 6 figures

International audience; Metastasis, involving the spread of systemic cancer to the brain, results in neurologic disability and death. Current treatments are largely palliative in nature; improved therapeutic approaches represent an unmet clinical need. However, recent experimental and clinical advances challenge the bleak long-term outcome of this disease. Encompassing key recent findings in epidemiology, genetics, microenvironment, leptomeningeal disease, neurocognition, targeted therapy, immunotherapy, and prophylaxis, we review preclinical and clinical studies to provide a comprehensive picture of contemporary research and the management of secondary brain tumors.

Here, I focus on the use of microscopic, few-body techniques that are relevant in the many-body problem. These methods can be divided into indirect and direct. In particular, indirect methods are concerned with the simplification of the many-body problem by substituting the full, microscopic interactions by pseudopotentials which are designed to reproduce collisional information at specified energies, or binding energies in the few-body sector. These simplified interactions yield more tractable theories of the many-body problem, and are equivalent to effective field theory of interactions. Direct methods, which so far are most useful in one spatial dimension, have the goal of attacking the many-body problem at once by using few-body information only. Here, I will present non-perturbative direct methods to study one-dimensional fermionic and bosonic gases in one dimension., 22 pages, 0 figures. Lecture notes presented at Critical Stability of Quantum Few-Body Systems, Dresden, 9-16 October 2017, plus some new results on the subject

The discovery of genes and molecular pathways involved in the formation of brain metastasis would direct the development of therapeutic strategies to prevent this deadly complication of cancer. By comparing gene expression profiles of Estrogen Receptor negative (ER-) primary breast tumors between patients who developed metastasis to brain and to organs other than brain, we found that T lymphocytes promote the formation of brain metastases. To functionally test the ability of T cells to promote brain metastasis, we used an in vitro blood-brain barrier (BBB) model. By co-culturing T lymphocytes with breast cancer cells, we confirmed that T cells increase the ability of breast cancer cells to cross the BBB. Proteomics analysis of the tumor cells revealed Guanylate-Binding Protein 1 (GBP1) as a key T lymphocyte-induced protein that enables breast cancer cells to cross the BBB. The GBP1 gene appeared to be up-regulated in breast cancer of patients who developed brain metastasis. Silencing of GBP1 reduced the ability of breast cancer cells to cross the in vitro BBB model. In addition, the findings were confirmed in vivo in an immunocompetent syngeneic mouse model. Co-culturing of ErbB2 tumor cells with activated T cells induced a significant increase in Gbp1 expression by the cancer cells. Intracardial inoculation of the co-cultured tumor cells resulted in preferential seeding to brain. Moreover, intracerebral outgrowth of the tumor cells was demonstrated. The findings point to a role of T cells in the formation of brain metastases in ER- breast cancers, and provide potential targets for intervention to prevent the development of cerebral metastases.

cited By 0; International audience; We have examined the potential of discriminant inorganic constituents (trace‐, ultra‐trace elements and Sr isotope ratios) to assess the origin of world famous brands of European dry‐cured hams. The variation of the multielemental composition with principal component analysis allowed to discriminate the origin of Bayonne hams. Determined ratio 87Sr/86Sr was recognized as a strong additional distinctive parameter. The ratio 87Sr/86Sr allowed to better separate all the different categories of hams in addition to the multi‐elemental detection. The major contribution of the value 87Sr/86Sr for the Bayonne ham is directly related to its curing due to the salt used in process coming from the nearby salt mine Salies‐de‐Béarn. Since the salt represents around 4% of the final product, it will therefore strongly influence the elemental and isotopic composition of hams. The overall discrimination potential of strontium isotope ratio is evidenced in the final statistical discrimination of the origin of hams.

We herein combined experimental and computational efforts to delineate the mechanism of action through which the flavonolignan silibinin targets STAT3. Silibinin reduced IL-6 inducible, constitutive, and acquired feedback activation of STAT3 at tyrosine 705 (Y705). Silibinin attenuated the inducible phospho-activation of Y705 in GFP-STAT3 genetic fusions without drastically altering the kinase activity of the STAT3 upstream kinases JAK1 and JAK2. A comparative computational study based on docking and molecular dynamics simulation over 14 different STAT3 inhibitors (STAT3i) predicted that silibinin could directly bind with high affinity to both the Src homology-2 (SH2) domain and the DNA-binding domain (DBD) of STAT3. Silibinin partially overlapped with the cavity occupied by other STAT3i in the SH2 domain to indirectly prevent Y705 phosphorylation, yet showing a unique binding mode. Moreover, silibinin was the only STAT3i predicted to establish direct interactions with DNA in its targeting to the STAT3 DBD. The prevention of STAT3 nuclear translocation, the blockade of the binding of activated STAT3 to its consensus DNA sequence, and the suppression of STAT3-directed transcriptional activity confirmed silibinin as a direct STAT3i. The unique characteristics of silibinin as a bimodal SH2- and DBD-targeting STAT3i make silibinin a promising lead for designing new, more effective STAT3i. This work was supported by grants from the Ministerio de Ciencia e Innovacion (Grant SAF2016-80639-P to J. A. Menendez), Plan Nacional de I + D + I, Spain, and the Agencia de Gestio d'Ajuts Universitaris i de Recerca (AGAUR) (Grant 2014 SGR229 to J. A. Menendez). This study was supported also by unrestricted research grants from Roche Pharma (Spain) and Astellas Pharma (Spain) to the Program Against Cancer Therapeutic Resistance (ProCURE, Catalan Institute of Oncology). Joaquim Bosch-Barrera is supported by SEOM, Pfizer (Grant WI190764), Boehringer Ingelheim, Meda Pharma, and Pla strategic de recerca i innovacio en salut 2016-2020 de la Generalitat de Catalunya (SLT006/17/114). Elisabet Cuyas is supported by a Sara Borrell postdoctoral contract (CD15/00033) from the Ministerio de Sanidad y Consumo, Fondo de Investigacion Sanitaria (FIS), Spain. The authors would like to thank Dr. Kenneth McCreath for editorial support. Sí

The adsorption of Pt(IV) by iron oxide (Fe3O4) superparamagnetic nanoparticles (SPION) functionalized with 3-mercaptopropionic acid (3-MPA) is investigated by means of inductively coupled plasma optical emission spectrometry and isothermal titration calorimetry. Experimental ICP data are better fitted by Langmuir rather than Freundlich isotherms. The whole thermodynamic parameters and maximum loading capacity for the adsorption process of Pt(IV) on the functionalized SPION nanoparticles (SPION@3-MPA) are obtained. The process is enthalpy-driven, while entropy is largely unfavourable suggesting that some other interaction should be present in addition to the electrostatic ones with the coverage surface. When compared to other thiol-functionalized materials, the SPION@3-MPA can be considered an interesting adsorbent for Pt(IV), especially with respect to the short contact time evidenced.

Introduction Flavonoids are polyphenolic compounds found ubiquitously in foods of plant origin. They are commonly extracted from plant materials with ethanol, methanol, water, their combination or even with acidified extracting solutions. The disadvantages of these methods are the use of high quantity of organic solvent, the possible loss of analytes in the different steps and the laborious process of the techniques. In addition, the complexity of the phenolic mixtures present in plant materials requires a preliminary clean-up and fractionation of the crude extracts. Objective To develop a hollow fibre liquid phase micro-extraction (HF-LPME) method for a one step clean-up and pre-concentration of flavonoids. Methodology Two flavonoids (catechin and rutin) has been extracted by HF-LPME and analysed by HPLC. The related driving force for the liquid membrane has been studied by means of facilitated and non-facilitated transport. Different ionic and non-ionic water insoluble compounds [trioctylamine (TOA), tributyl phosphate (TBP), trioctylphosphine oxide (TOPO) and methyltrioctylammonium chloride (aliquat 336)] were used as carriers. The liquid membrane was constituted by a solution of n-decanol in the presence or absence of carriers. Results Maximum enrichment factors were obtained with n-decanol/aliquat 336 (20%) as organic liquid membrane, sodium hydroxide (NaOH) (0.1 M) as donor solution, sodium chloride (NaCl) (2 M) as acceptor solution and 3 h as extraction time. Under these conditions, good results for validation parameters were obtained [for linearity, limit of detection (LOD), limit of quantitation (LOQ) and repeatability]. Conclusions The developed method is simple, effective and has been successfully applied to determine catechin and rutin in ethanolic extracts of faba beans. Copyright © 2015 John Wiley & Sons, Ltd.

The appearance of cerebral metastasis usually defines the terminal stage of disease for women suffering from breast cancer. Breast cancer dissemination to brain tumor cells need to take the hurdle of the blood-brain barrier (BBB).

Contaminants of Emerging Concerns (CECs) have been introduced as one type of recalcitrant pollutant sources in water. In this study, the non-steroidal anti-inflammatory drug diclofenac (DCF) has been removed from water solutions using Molecularly Imprinted Polymer (MIP), synthetized via bulk polymerization with allylthiourea (AT) as the functional monomer and using DCF as template (MIP-DCF). DCF detection has been performed by UV spectrophotometer. From the kinetic study in batch mode, approximately 100% of removal is observed by using 10 mg of MIP-DCF, with an initial concentration of 5 mg/L of DCF at pH 7, within 3 min and agitated at 25 °C. In continuous flow mode study, using a cartridge pre-packed with 10 mg of MIP-DCF, a high adsorption capacity of 160 mg DCF/g MIP was obtained. To study the porosity of MIPs, scanning electron microscopy (SEM) has been used. In order to characterize the chemical interaction between monomer and template, the pre-polymerization mixture for MIP and DCF has also been studied by H NMR. One of the chemical shift observed has been related to the formation of a complex between amine protons of thiourea group of AT with carboxylic acid on DCF. In conclusion, the developed MIP works as a good adsorbent for DCF removal, and is selective to DCF in the presence of indomethacin and ibuprofen.

Luttinger liquid theory is a powerful and widely applicable framework for modelling one-dimensional many-body quantum systems. Within this framework, one supposes that the macroscopic behaviour of such systems is entirely determined by two phenomenological parameters, g(2) and g(4). While there exists an intuitive and seemingly sensible physical interpretation of these parameters in terms of the scattering of the system's constituent particles, g(2) and g(4) are traditionally either fixed by experiment or calculated using heavy-duty numerical techniques, rather than inferred using scattering theory, and for this reason the interpretation remains untested. By applying Luttinger liquid theory in a simple setting, we show that a widely-held and repeatedly-stated belief, namely that the intrabranch terms appearing in Luttinger's model originate from microscopic intrabranch interactions, is a misconception. We begin with the microscopic model of an interacting one-dimensional, spin-polarized Fermi gas, which we systematically transform into a Luttinger model by introducing an effective interaction, linearizing the dispersion, and renormalizing. By this method, we are able to show that the usual interpretation of g(4) as a measure of intrabranch scattering implies that it must vanish in the dilute limit. Since this runs contrary to conservation of particle number, we conclude that g(4) cannot be related to intrabranch scattering. Rather, we show that g(4) interactions must be included in the effective model in order to compensate for the deleterious effect that introducing an effective interaction has upon the model's energetics. We explicitly calculate an approximation to this correction for our simple system, and find that it agrees with the value of g(4) found in the literature. We therefore propose a new fermionic Hamiltonian which agrees with the traditional model after bosonisation, but which better reflects the underlying microscopic physics.

The brain microenvironment imposes a particularly intense selective pressure on metastasis-initiating cells, but successful metastases bypass this control through mechanisms that are poorly understood. Reactive astrocytes are key components of this microenvironment that confine brain metastasis without infiltrating the lesion. Here, we describe that brain metastatic cells induce and maintain the co-option of a pro-metastatic program driven by signal transducer and activator of transcription 3 (STAT3) in a subpopulation of reactive astrocytes surrounding metastatic lesions. These reactive astrocytes benefit metastatic cells by their modulatory effect on the innate and acquired immune system. In patients, active STAT3 in reactive astrocytes correlates with reduced survival from diagnosis of intracranial metastases. Blocking STAT3 signaling in reactive astrocytes reduces experimental brain metastasis from different primary tumor sources, even at advanced stages of colonization. We also show that a safe and orally bioavailable treatment that inhibits STAT3 exhibits significant antitumor effects in patients with advanced systemic disease that included brain metastasis. Responses to this therapy were notable in the central nervous system, where several complete responses were achieved. Given that brain metastasis causes substantial morbidity and mortality, our results identify a novel treatment for increasing survival in patients with secondary brain tumors. Reactive astrocytes expressing STAT3 support the metastatic growth of tumor cells colonizing the brain and can be pharmacologically targeted to improve the clinical management of patients with secondary brain tumors.

Long-range interactions and, in particular, two-body potentials with power-law long-distance tails are ubiquitous in nature. For two bosons or fermions in one spatial dimension, the latter case being formally equivalent to three-dimensional $s$-wave scattering, we show how generic asymptotic interaction tails can be accounted for in the long-distance limit of scattering wave functions. This is made possible by introducing a generalisation of the collisional phase shifts to include space dependence. We show that this distance dependence is universal, in that it does not depend on short-distance details of the interaction. The energy dependence is also universal, and is fully determined by the asymptotic tails of the two-body potential. As an important application of our findings, we describe how to eliminate finite-size effects with long-range potentials in the calculation of scattering phase shifts from exact diagonalisation. We show that even with moderately small system sizes it is possible to accurately extract phase shifts that would otherwise be plagued with finite-size errors. We also consider multi-channel scattering, focusing on the estimation of open channel asymptotic interaction strengths via finite-size analysis., 7 pages, 3 figures

We consider quantum scattering of particles in media exhibiting strong dispersion degeneracy. In particular, we study flat-banded lattices and linearly dispersed energy bands. The former constitute a prime example of single-particle frustration while the latter show degeneracy at the few- and many-particle level. We investigate both impurity and two-body scattering and show that, quite generally, scattering does not occur, which we relate to the fact that transition matrices vanish on the energy shell. We prove that scattering is instead replaced by projections onto band-projected eigenstates of the interaction potential. We then use the general results to obtain localised flat band states that are eigenstates of impurity potentials with vanishing eigenvalues in one-dimensional flat bands and study the particular case of a sawtooth lattice. We also uncover the relation between certain solutions of one-dimensional systems that have been categorised as "strange", and the scattering states in linearly dispersed continuum systems., 7 pages, 0 figures. Updated references

Recently, the topics of many-body localization (MBL) and one-dimensional strongly interacting few-body systems have received a lot of interest. These two topics have been largely developed separately. However, the generality of the latter as far as external potentials are concerned -- including random and quasirandom potentials -- and their shared spatial dimensionality, makes it an interesting way of dealing with MBL in the strongly interacting regime. Utilising tools developed for few-body systems we look to gain insight into the localization properties of the spin in a Fermi gas with strong interactions. We observe a delocalized--localized transition over a range of fillings of a quasirandom lattice. We find this transition to be of a different nature for low and high fillings, due to the diluteness of the system for low fillings., 6 pages, 3 figures

The thermodynamic parameters for the complexation between the monodentate diethylsulfide (Et2S), the macrocyclic thioethers 1,4,7-trithiacyclononane ([9]aneS3) 1,4,7,10-tetrathiacyclododecane ([12]aneS4) and 1,4,8,11-tetrathiacyclotetradecane ([14]aneS4) have been determined in dimethylsulfoxide (DMSO) with d10 metal ions such as Hg2+, Ag+, Cd2+and Zn2+. Mononuclear MLj (j = 1, 2) complexes are formed by all ligands with Hg2+ and Ag+ with associated negative enthalpy and entropy changes. Differently from what previously found in acetonitrile (AN), no reaction occurs with Zn2+ and Cd2+. The stabilities are lower in DMSO and the selectivity, much higher for Hg2+ than Ag+ in AN, is leveled in DMSO, being higher only when [9]aneS3 is concerned (Δlog K1,Hg → Ag = 8.9 and 0.9 for [9]aneS3 in AN and DMSO, respectively). Density Functional Theory (DFT) calculations are used to interpret at molecular level the subtle interplay between the intrinsic affinity of a given metal ion for a thioether and solvation in different media. The reliability of this approach is demonstrated by the good correlation obtained for the calculated and theoretical solvation energy of the metal ions. Theoretical results clearly indicate that: (i) the medium (solvent) strongly modulates the relative selectivity of thioethers towards different cations, i.e. Hg2+ and Ag+; (ii) Zn2+ complexes are not formed in DMSO mostly because of the strong solvation of the ion; (iii) for Cd2+ the less favorable pure interaction with the ligands is most detrimental to complex formation.

SummaryBrain metastasis is an ominous complication of cancer, yet most cancer cells that infiltrate the brain die of unknown causes. Here, we identify plasmin from the reactive brain stroma as a defense against metastatic invasion, and plasminogen activator (PA) inhibitory serpins in cancer cells as a shield against this defense. Plasmin suppresses brain metastasis in two ways: by converting membrane-bound astrocytic FasL into a paracrine death signal for cancer cells, and by inactivating the axon pathfinding molecule L1CAM, which metastatic cells express for spreading along brain capillaries and for metastatic outgrowth. Brain metastatic cells from lung cancer and breast cancer express high levels of anti-PA serpins, including neuroserpin and serpin B2, to prevent plasmin generation and its metastasis-suppressive effects. By protecting cancer cells from death signals and fostering vascular co-option, anti-PA serpins provide a unifying mechanism for the initiation of brain metastasis in lung and breast cancers.

The diagnosis of brain metastasis involves high morbidity and mortality and remains as an unmet clinical need in spite of being the most common tumor in the brain. Brain metastasis affects between 10-30% of cancer patients with 200.000 new cases yearly only in the US. Increasing evidences point out the relevance of the microenvironment to understand the biology of brain colonization by metastatic cells, however translation strategies targeting it are lacking. We have identified altered signaling pathways in pro-metastatic reactive astrocytes (RA), and translated that into a novel therapeutic application for brain metastasis. Specifically we have identified a subpopulation of RA characterized by activated STAT3 pathway (pSTAT3+). This subpopulation is located in the vicinity of metastatic lesions intermingled with pSTAT3- RA in several experimental models and in 89% of human brain metastases independently of the primary tumor source. The pro-metastatic behaviour of RA is regulated by the activation of the STAT3 pathway, which modulates the immune system locally promoting the survival of cancer cells. Specifically the secretome of pSTAT3+ RA decreases the anti-tumor activity of CD8+ T cells as well as promotes the expansion of the pro-tumor population of CD74+ microglia/macrophage, which infiltrates metastasis cores. Interestingly, the immunosuppressive nature of pSTAT3+ RA is linked to the acquisition of stem cell-like properties, which might reflect the misuse of brain responses to injury instigated by the presence of cancer cells. Genetic and pharmacologic approaches targeting STAT3 in RA impair the progression of brain metastasis, even at advanced stages of the disease. Moreover, a safe and orally bioavailable STAT3 inhibitor reduced brain metastasis in 75% of 18 stage IV lung adenocarcinoma patients with established brain metastases, improving the outcome of the disease by increasing patient survival from 4 to 15 months. Besides increasing therapeutic opportunities for patients with brain metastasis, we have described for the first time the role of reactive astrocytes as regulators of local immunosuppression in brain metastasis as well as the importance of uncovering the heterogeneity within the metastasis-associated microenvironment. Citation Format: Neibla Priego, Lucía Zhu, Catia Monteiro, Manon Mulders, David Wasilewski, Wendy Bindeman, Laura Doglio, Elena Martínez-Saez, Santiago Ramón y Cajal, Coral Fustero-Torre, Elena Piñeiro-Yáñez, Aurelio Hernández-Laín, Valeria Poli, Javier A. Menéndez, Ricardo Soffietti, Joaquim Bosch-Barrera, Manuel Valiente. Stat3 labels a subpopulation of reactive astrocytes required for brain metastasis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 2746.

Purpose: Brain metastasis remains a major site of relapse for several cancer types. However, the molecular mechanisms of brain metastasis are largely unknown given the complex relationship between tumor cells and the surrounding brain tumor microenvironment (TME). To better characterize the tumor-stromal relationship in the context of brain metastasis, we have developed a Brain Metastatic RNA-Sequencing (BMX-Seq) approach, which leverages xenograft models and can distinguish between the transcriptomes of human tumor and mouse stroma gene expression in vivo. This resource provides an extensive molecular portrait of the co-adaptation of brain metastasis with the brain TME. Methods: Following intra-arterial injection of the human, lung adenocarcinoma (LUAD), H2030-BrM3 cell model into athymic mice, metastatic brain tumors were macrodisasected, flash frozen and RNA extracted. Healthy brain regions, subcutaneous tumors and H2030-BrM3 cells grown in monolayer were harvested in parallel. Our BMX-Seq pipeline was engineered to analyze bulk xenograft tissue, which includes tumor lesions as well as surrounding stromal tissue. Species-specific Taqman primers and immunofluorescent (IF) staining were used to validate our BMX-Seq results. Models of melanoma and breast cancer brain metastasis were also included in this study. Summary: In comparing the transcriptomic profiles of tumors grown in the brain as compared to other sites, we identify shifts in epithelial and neuronal-like gene expression programs in malignant cells and show such trends are reversible once tumor cells are removed from the brain TME. Genes involved in WNT signaling and cell projection were also upregulated across melanoma, lung and breast cancer types when cells were grown in the brain versus other sites. In describing the neuroinflammatory response of stromal regions directly surrounding brain lesions, our BMX-Seq analysis revealed increased expression of astrocyte and microglial enriched transcripts. We then utilized IF to confirm the elevated density of both of these cell types in areas directly surrounding and infiltrating tumor regions. We also detect significantly induced expression of stromal TIM3 in areas surrounding tumor lesions and in a model system devoid of T-Cells. Follow-up work revealed TIM3 is largely expressed and induced on tumor-associated macrophages (including microglia) within the tumor region, and we further validate such results in a syngeneic model system as well as in human, brain tissue. Conclusions: We have developed a highly sensitive RNA-sequencing based approach that can accurately map the transcriptomic adaptation of brain metastatic tumor cells and the surrounding brain TME. We believe these data may serve as an invaluable resource to guide future discovery in CNS metastases. Citation Format: Emily Wingrove, Zongzhi Z. Liu, Don X. Nguyen, Anna Arnal-Estape, Kiran D. Patel, Mary-Ann Melnick, Katerina Politi, Manuel Valiente, Harriet M. Kluger, Veronica L. Chiang. BMX-Seq as a new resource for deciphering the transcriptomic hallmarks of tumor plasticity and stromal interactions in brain metastasis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 4552.

The brain is a major site of relapse for several cancers, yet deciphering the mechanisms of brain metastasis remains a challenge because of the complexity of the brain tumor microenvironment (TME). To define the molecular landscape of brain metastasis from intact tissue in vivo, we employ an RNA-sequencing-based approach, which leverages the transcriptome of xenografts and distinguishes tumor cell and stromal gene expression with improved sensitivity and accuracy. Our data reveal shifts in epithelial and neuronal-like lineage programs in malignant cells as they adapt to the brain TME and the reciprocal neuroinflammatory response of the stroma. We identify several transcriptional hallmarks of metastasis that are specific to particular regions of the brain, induced across multiple tumor types, and confirmed in syngeneic models and patient biopsies. These data may serve as a resource for exploring mechanisms of TME co-adaptation within, as well as across, different subtypes of brain metastasis. We thank Sally Adua for critical review of the manuscript. This work wasfunded by grants R01CA166376, R01CA191489, and W81XWH-15-1-0117and the Beatrice Kleinberg Neuwirth Fund (to D.X.N.); Yale Lung CancerSPORE grant P50CA196530 (to K.P. and D.X.N.); Yale SPORE in Skin Cancergrants P50 CA121974, K24CA172123, and R01 CA216846 (to H.M.K.);MINECO grants SAF2017-89643-R and SAF2015-62547-ERC, Bristol-MyersSquibb-MRA Young Investigator Award 2017, Fundacio ́n Ramo ́n Areces,and CLIP CRI Award 2018 (to M.V.); La Caixa-Severo Ochoa InternationalPhD Program Fellowship (to L.Z.); and FCT PhD Fellowship SFRH/BD/100089/2014 (to C.M.). M.V. is a Ramo ́n y Cajal Investigator (RYC-2013-13365) and member of EMBO YIP (4053). K.D.P. was supported by grantT32 CA193200, W.L.C. by grant DGE-1122492, and E.W. by grant T32GM100884 Sí

In the version of this Article originally published, the authors inadvertently included the term 'pericytic mimicry' in relation to ref. 54. This has now been corrected by inserting an additional reference at position 51 and amending the text in the Discussion relating to 'pericytic mimicry', ref. 54 and pericyte-like spreading. The original refs 51-70 have also been renumbered. Furthermore, Fig. 8l has been amended to remove the term 'pericyte mimicry' that the authors had included inadvertently during figure preparation. These corrections have been made in the online versions of the Article.

Spin chains are paradigmatic systems for the studies of quantum phases and phase transitions, and for quantum information applications, including quantum computation and short-distance quantum communication. Here we propose and analyse a scheme for conditional state transfer in a Heisenberg XXZ spin chain which realizes a quantum spin transistor. In our scheme, the absence or presence of a control spin excitation in the central gate part of the spin chain results in either perfect transfer of an arbitrary state of a target spin between the weakly coupled input and output ports, or its complete blockade at the input port. We also discuss a possible proof-of-concept realization of the corresponding spin chain with a one-dimensional ensemble of cold atoms with strong contact interactions. Our scheme is generally applicable to various implementations of tunable spin chains, and it paves the way for the realization of integrated quantum logic elements., Systems of interacting quantum spins provide a basis for quantum computation devices. Here, the authors demonstrate a quantum spin transistor in a Heisenberg spin chain, which may be realized in a system of trapped cold atoms.

We consider the low-energy particle-particle scattering properties in a periodic simple cubic crystal. In particular, we investigate the relation between the two-body scattering length and the energy shift experienced by the lowest-lying unbound state when this is placed in a periodic finite box. We introduce a continuum model for s-wave contact interactions that respects the symmetry of the Brillouin zone in its regularisation and renormalisation procedures, and corresponds to the naive continuum limit of the Hubbard model. The energy shifts are found to be identical to those obtained in the usual spherically symmetric renormalisation scheme upon resolving an important subtlety regarding the cutoff procedure. We then particularize to the Hubbard model, and find that for large finite lattices the results are identical to those obtained in the continuum limit. The results reported here are valid in the weak, intermediate and unitary limits. These may be used to significantly ease the extraction of scattering information, and therefore effective interactions in condensed matter systems in realistic periodic potentials. This can achieved via exact diagonalisation or Monte Carlo methods, without the need to solve challenging, genuine multichannel collisional problems with very restricted symmetry simplifications.

Topological quantum matter can be realized by subjecting engineered systems to time-periodic modulations. In analogy with static systems, periodically driven quantum matter can be topologically classified by topological invariants, whose non-zero value guarantees the presence of robust edge modes. In the high-frequency limit of the drive, topology is described by standard topological invariants, such as Chern numbers. Away from this limit, these topological numbers become irrelevant, and novel topological invariants must be introduced to capture topological edge transport. The corresponding edge modes were coined anomalous topological edge modes, to highlight their intriguing origin. Here we demonstrate the experimental observation of these topological edge modes in a 2D photonic lattice, where these propagating edge states are shown to coexist with a quasi-localized bulk. Our work opens an exciting route for the exploration of topological physics in time-modulated systems operating away from the high-frequency regime., SCOPUS: ar.j, info:eu-repo/semantics/published

We report on the experimental realization of a photonic system that simulates the one-dimensional two-particle Hubbard model. This analogy is realized by means of two-dimensional arrays of coupled optical waveguides, fabricated using femtosecond laser inscription. By tuning the analogous "interaction strength", we reach the strongly-interacting regime of the Hubbard Hamiltonian, and demonstrate the suppression of standard tunneling for individual "particles". In this regime, the formation of bound states is identified through the direct observation of pair tunneling. We then demonstrate the coherent destruction of tunneling (CDT) for the paired particles in the presence of an engineered oscillating force of high frequency. The precise control over the analogous "interaction strength" and driving force offered by our experimental system opens an exciting route towards quantum simulation of few-body physics in photonics., 4 pages, 4 figures, Appendix

Gapless many-body quantum systems in one spatial dimension are universally described by the Luttinger liquid effective theory at low energies. Essentially, only two parameters enter the effective low-energy description, namely the speed of sound and the Luttinger parameter. These are highly system dependent and their calculation requires accurate non-perturbative solutions of the many-body problem. Here, we present a simple method that only uses collisional information to extract the low-energy properties of these systems. Our results are in remarkable agreement with available results for integrable models and from large scale Monte Carlo simulations of one-dimensional helium and hydrogen isotopes. Moreover, we estimate theoretically the critical point for spinodal decomposition in one-dimensional helium-4, and show that the exponent governing the divergence of the Luttinger parameter near the critical point is exactly 1/2, in excellent agreement with Monte Carlo simulations., Comment: 8 pages, 6 figures, including supplementary material

Periodically-driven quantum systems can exhibit topologically non-trivial behaviour, even when their quasi-energy bands have zero Chern numbers. Much work has been conducted on non-interacting quantum-mechanical models where this kind of behaviour is present. However, the inclusion of interactions in out-of-equilibrium quantum systems can prove to be quite challenging. On the other hand, the classical counterpart of hard-core interactions can be simulated efficiently via constrained random walks. The non-interacting model proposed by Rudner et al. [Phys. Rev. X 3, 031005 (2013)], has a special point for which the system is equivalent to a classical random walk. We consider the classical counterpart of this model, which is exact at a special point even when hard-core interactions are present, and show how these quantitatively affect the edge currents in a strip geometry. We find that the interacting classical system is well described by a mean-field theory. Using this we simulate the dynamics of the classical system, which show that the interactions play the role of Markovian, or time dependent disorder. By comparing the evolution of classical and quantum edge currents in small lattices, we find regimes where the classical limit considered gives good insight into the quantum problem., Comment: 15 pages, 15 figures, new content on the quantum model

Novel nanocomposite sorbents of superparamagnetic iron oxide nanoparticles (SPION) supported onto electrospun polyacrylonitrile nanofibers were synthesized by a simple and scalable method. The influence of both nanofiber size and SPION loading on As(V) adsorption capacity was studied and optimization was conducted. A maximum uptake capacity of 32.5 mmol As(V) per gram SPION in batch mode tests using an extremely low loading of only 2.9 mg of SPION per gram of adsorbent was achieved. This represents a remarkable improvement of 36 times compared with SPION in suspension. The optimal material was tested in continuous flow operation mode, reaching an adsorption capacity of 851.7 mg As(V) per gram of adsorbent at pH 3.8. It is also demonstrated that the new adsorbents can retain high performance when tested under real conditions using polluted wastewater from a lixiviation dump containing a large amount of competing anions (Cl- and F- ) and interfering cations (K+, Na+, Mg2+, and Ca2+). Furthermore, there was no release of nanoparticles observed during the operation and the spent porous material can be compressed, generating a small amount of solid waste that can be easily treated or stored.

The present study represents a first insight into the Draa Lasfar mine (Marrakech) to assess the possible diffusion of heavy metals and to predict the risk of their mobility in the surroundings of the mine area. The edaphologic parameters pH, electrical conductivity (EC), loss on ignition (LOI) and CaCO 3 were measured according to standard methods, whilst heavy metals concentration was determined by FP-XRF. Concentration enrichment ratios (CER) were calculated in order to estimate the anthropogenic contribution of target pollutants determining As, Cu, Pb and Zn as the main pollutants, whereas Ba, Ca, Fe, K, Mn, Rb, Sr, Ti and Zr were considered lithogenic components. GIS contour maps of pollutants using CER data, showed the most polluted areas at the vicinity of the mine, especially at the northwest area, probably linked to weathering effects and topography of the area. Particle size studies revealed that As, Pb and Zn are part of the mineral ore while Cu behavior corresponded to an anthropogenic origin. Additionally, mobility assays employing single leaching tests indicated a greater mobility of As and Zn rather than that of Pb and Cu due to their lower adsorption process in the soil, independently of their respective concentration.

A stream of effluent from a mine tailings pond, containing zinc, ferrous ions and other metals, required treatment to prevent pollution and recover valuable metals. A solvent extraction (SX) process using Ionquest 290 as extractant was developed to recover the Zn from the effluent. Ferrous ions were bio-oxidized and removed by selective alkaline precipitation prior to the zinc extraction. The Fe removal as well as the SX process were developed successfully at laboratory scale and verified in a pilot plant on-site, using two Bateman Pulsed Columns for the extraction and stripping of Zn. The results were satisfactory obtaining above 95% recovery of the Zn.

The feasibility of three commercial extractants (DEHPA, Cyanex 272, and Ionquest 290) has been assessed for the recovery of Zn from an acidic mine effluent. Less than 5 min are required to reach equilibrium for the studied extractants. Regarding selectivity, DEHPA extracted efficiently Zn, Ca, Mn, and Al, although Al remained in the solvent extract after stripping, hindering the solvent reuse. Neither Ionquest 290 nor Cyanex 272 extract Al, Cu, Mn, or Ca significantly. Ionquest 290 recovery of Zn is 5–10% higher than Cyanex 272. In addition, 20%(v/v) Ionquest 290 produces higher recoveries than 40%(v/v) DEHPA, thus Ionquest 290 has been selected as the most suitable among the extractants studied.

In vitro antioxidant capacity of thirteen genotypes of faba bean (Vicia faba L.) collected during three different growing stages (vegetative, reproductive and maturity) have been evaluated. For the vegetative stage (VS) and reproductive stage (RS) the whole plant (WP) has been used for the analysis while for the maturity stage (MS) the plant was divided and analyzed in different parts, the whole seed (WS), seed coat (SC), cotyledons (Cot) and pod (Pod). The antioxidant capacity has been evaluated by means of the total phenolic and total flavonoid contents (TPC and TFC respectively), the ferric-reducing/antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assays. The results showed different antioxidant properties for the same genotype when considering the different parts or stages of the plant. High content of both total phenolics (> 92.85 mg as gallic acid equivalent (GAE)/g of dried plant) and flavonoids contents (> 11.87 mg as rutin equivalent (RE)/g of dried plant) were found for all genotypes during the vegetative and the reproductive stages, which also showed the highest antioxidant activity (FRAP value ≥ 1.157 mmol/g and DPPH radical-scavenging capacity > 43.49%), indicating their potential as natural sources of antioxidant foods. For the maturity stage, the highest values for the TPC, TFC, FRAP and DPPH scavenging capacity were observed for the pods and the lowest for the cotyledons. The results of this study indicate that faba beans are a good source of natural antioxidants independently to their genotype. The quantification of the antioxidant capacity according to the stage and the plant part could be suitable for applications on the food industry in relation to production and preservation of faba bean food products.