101. Sesquiterpene Lactones Isolated from Elephantopus scaber L. Inhibits Human Lymphocyte Proliferation and the Growth of Tumour Cell Lines and Induces Apoptosis In Vitro
- Author
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P. G. Latha, B. S. Geetha, P. Remani, and Mangalam S. Nair
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Male ,Article Subject ,Cell Survival ,Health, Toxicology and Mutagenesis ,lcsh:Biotechnology ,lcsh:Medicine ,Antineoplastic Agents ,Apoptosis ,Asteraceae ,Biology ,Lymphocyte Activation ,Sesquiterpene lactone ,Lactones ,Mice ,lcsh:TP248.13-248.65 ,Genetics ,Animals ,Humans ,Cytotoxic T cell ,Lymphocytes ,Viability assay ,Molecular Biology ,Cells, Cultured ,Cell Proliferation ,chemistry.chemical_classification ,Dose-Response Relationship, Drug ,Plant Extracts ,Cell growth ,lcsh:R ,DNA ,General Medicine ,HCT116 Cells ,biology.organism_classification ,Survival Analysis ,Molecular biology ,In vitro ,Elephantopus scaber ,chemistry ,Cell culture ,Immunology ,Molecular Medicine ,Sesquiterpenes ,Research Article ,Biotechnology - Abstract
This study was designed to isolate the compounds responsible for the cytotoxic properties of South IndianElephantopus scaberL. and further investigate their effects on quiescent and proliferating cells. Bioassay-guided isolation of the whole plant of chloroform extract of South IndianElephantopus scaberafforded the known sesquiterpene lactone, deoxyelephantopin, and isodeoxyelephantopin whose structures were determined by spectroscopic methods. These compounds caused a dose dependent reduction in the viability of L-929 tumour cells in 72 h culture (IC50value of 2.7 μg/mL and 3.3 μg/mL) by the cell viability assay. Both the compounds act selectively on quiescent and PHA-stimulated proliferating human lymphocytes and inhibited tritiated thymidine incorporation into cellular DNA of DLA tumour cells. The compound deoxyelephantopin at a concentration of 3 μg/mL caused maximum apoptotic cells. It also exhibited significantin vivoantitumour efficacy against DLA tumour cells. The results, therefore, indicate that the antiproliferative property of deoxyelephantopin and isodeoxyelephantopin could be used in regimens for treating tumors with extensive proliferative potencies.
- Published
- 2012
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