101. Automated Microfluidic Filtration and Immunocytochemistry Detection System for Capture and Enumeration of Circulating Tumor Cells and Other Rare Cell Populations in Blood.
- Author
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Pugia M, Magbanua MJM, and Park JW
- Subjects
- Antibodies, Monoclonal chemistry, Antibodies, Monoclonal metabolism, Biomarkers, Tumor genetics, Biomarkers, Tumor immunology, Biomarkers, Tumor metabolism, Cell Line, Tumor, Cell Separation instrumentation, Cell Size, Endothelial Cells cytology, Endothelial Cells metabolism, Epithelial Cell Adhesion Molecule genetics, Epithelial Cell Adhesion Molecule immunology, Epithelial Cell Adhesion Molecule metabolism, Filtration instrumentation, Fluorescent Antibody Technique methods, Fluorescent Dyes chemistry, Humans, Immunoconjugates chemistry, Keratins genetics, Keratins immunology, Keratins metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Neoplasms blood, Neoplasms immunology, Neoplasms pathology, Neoplastic Cells, Circulating immunology, Neoplastic Cells, Circulating metabolism, Neoplastic Stem Cells immunology, Neoplastic Stem Cells metabolism, Neoplastic Stem Cells pathology, Protein Binding, Rheology, Cell Separation methods, Equipment Design, Filtration methods, Microfluidic Analytical Techniques instrumentation, Neoplasms diagnosis, Neoplastic Cells, Circulating pathology
- Abstract
Isolation by size using a filter membrane offers an antigen-independent method for capturing rare cells present in blood of cancer patients. Multiple cell types, including circulating tumor cells (CTCs), captured on the filter membrane can be simultaneously identified via immunocytochemistry (ICC) analysis of specific cellular biomarkers. Here, we describe an automated microfluidic filtration method combined with a liquid handling system for sequential ICC assays to detect and enumerate non-hematologic rare cells in blood.
- Published
- 2017
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