Search

Efficient diagnosis of apple diseases and pests is crucial to the healthy development of the apple industry. However, the existing single-source image-based classification methods have limitations due to the constraints of single-source input image information, resulting in low classification accuracy and poor stability. Therefore, a classification method for apple disease and pest areas based on multi-source image fusion is proposed in this paper. Firstly, RGB images and multispectral images are obtained using drones to construct an apple diseases and pests canopy multi-source image dataset. Secondly, a vegetation index selection method based on saliency attention is proposed, which uses a multi-label ReliefF feature selection algorithm to obtain the importance scores of vegetation indices, enabling the automatic selection of vegetation indices. Finally, an apple disease and pest area multi-label classification model named AMMFNet is constructed, which effectively combines the advantages of RGB and multispectral multi-source images, performs data-level fusion of multi-source image data, and combines channel attention mechanisms to exploit the complementary aspects between multi-source data. The experimental results demonstrated that the proposed AMMFNet achieves a significant subset accuracy of 92.92%, a sample accuracy of 85.43%, and an F1 value of 86.21% on the apple disease and pest multi-source image dataset, representing improvements of 8.93% and 10.9% compared to prediction methods using only RGB or multispectral images. The experimental results also proved that the proposed method can provide technical support for the coarse-grained positioning of diseases and pests in apple orchards and has good application potential in the apple planting industry. [ABSTRACT FROM AUTHOR]

This paper addresses the problem of mapping land cover types in Senegal and recognition of vegetation systems in the Saloum River Delta on the satellite images. Multi-seasonal landscape dynamics were analyzed using Landsat 8-9 OLI/TIRS images from 2015 to 2023. Two image classification methods were compared, and their performance was evaluated in the GRASS GIS software (version 8.4.0, creator: GRASS Development Team, original location: Champaign, Illinois, USA, currently multinational project) by means of unsupervised classification using the k-means clustering algorithm and supervised classification using the Support Vector Machine (SVM) algorithm. The land cover types were identified using machine learning (ML)-based analysis of the spectral reflectance of the multispectral images. The results based on the processed multispectral images indicated a decrease in savannas, an increase in croplands and agricultural lands, a decline in forests, and changes to coastal wetlands, including mangroves with high biodiversity. The practical aim is to describe a novel method of creating land cover maps using RS data for each class and to improve accuracy. We accomplish this by calculating the areas occupied by 10 land cover classes within the target area for six consecutive years. Our results indicate that, in comparing the performance of the algorithms, the SVM classification approach increased the accuracy, with 98% of pixels being stable, which shows qualitative improvements in image classification. This paper contributes to the natural resource management and environmental monitoring of Senegal, West Africa, through advanced cartographic methods applied to remote sensing of Earth observation data. [ABSTRACT FROM AUTHOR]

Remote sensing technology is widely used to obtain information on floating green tides, and thresholding methods based on indices such as the normalized difference vegetation index (NDVI) and the floating algae index (FAI) play an important role in such studies. However, as the methods are influenced by many factors, the threshold values vary greatly; in particular, the error of data extraction clearly increases in situations of high-turbidity water (HTW) (NDVI > 0). In this study, high spatial resolution, multispectral images from the Sentinel-2 MSI mission were used as the data source. It was found that the International Commission on Illumination (CIE) hue angle calculated using remotely sensed equivalent multispectral reflectance data and the RGB method is extremely effective in distinguishing floating green tides from areas of HTW. Statistical analysis of Sentinel-2 MSI images showed that the threshold value of the hue angle that can effectively eliminate the effect of HTW is 218.94°. A test demonstration of the method for identifying the floating green tide in HTW in a Sentinel-2 MSI image was carried out using the identified threshold values of NDVI > 0 and CIE hue angle < 218.94°. The demonstration showed that the method effectively eliminates misidentification caused by HTW pixels (NDVI > 0), resulting in better consistency of the identification of the floating green tide and its distribution in the true color image. The method enables rapid and accurate extraction of information on floating green tide in HTW, and offers a new solution for the monitoring and tracking of green tides in coastal areas. [ABSTRACT FROM AUTHOR]

Using non-destructive evaluation tools based on imaging techniques, including single-sensor multispectral cameras, provides a cost-effective solution for optimizing rice nitrogen fertilization through site-specific nutrient management. However, their accuracy and precision have been identified as areas for improvement. This study aims to develop a methodology to improve the accuracy of estimations through field experiments. It utilizes multispectral images captured by MAPIR Survey3W Orange Cyan Near-Infrared and MAPIR Survey3W Red Edge cameras. The Normalized Difference Vegetation Index and Red Edge values derived from these images are correlated with Soil Plant Analysis Development values to assess rice nitrogen levels. A prediction model is then built using the Support Vector Regression algorithm. Findings from the experiments underscore the importance of addressing shadow effects, integrating the dataset on light intensity and image capture time, conducting radiometric calibration, filtering outlier data, employing image segmentation, and utilizing nonlinear Canova tests to enhance estimation accuracy. By configuring the Support Vector Regression model with RBF kernel, gamma set to 1.24, and epsilon set to 0.1, the R2 of the train data and validation data reaches 0.851, and 0.840 respectively. Meanwhile, the R2 of the test data achieves 0.793 with a mean absolute percentage error of 3.49% and a root mean square error of 1.70. These findings underscore the potential of the proposed methodology to improve the estimation of rice nitrogen status based on single-sensor multispectral images, paving the way for more effective nutrient management strategies in rice cultivation. [ABSTRACT FROM AUTHOR]

Old-growth forests are essential to preserve biodiversity and play an important role in sequestering carbon and mitigating climate change. However, their existence across Europe is vulnerable due to the scarcity of their distribution, logging, and environmental threats. Therefore, providing the current status of old-growth forests across Europe is essential to aiding informed conservation efforts and sustainable forest management. Remote sensing techniques have proven effective for mapping and monitoring forests over large areas. However, relying solely on remote sensing spectral or structural information cannot capture comprehensive horizontal and vertical structure complexity profiles associated with old-growth forest characteristics. To overcome this issue, we combined spectral information from Sentinel-2A multispectral imagery with 3D structural information from high-density point clouds of airborne laser scanning (ALS) imagery to map old-growth forests over an extended area. Four features from the ALS data and fifteen from Sentinel-2A comprising raw band (spectral reflectance), vegetation indices (VIs), and texture were selected to create three datasets used in the classification process using the random forest algorithm. The results demonstrated that combining ALS and Sentinel-2A features improved the classification performance and yielded the highest accuracy for old-growth class, with an F1-score of 92% and producer's and user's accuracies of 93% and 90%, respectively. The findings suggest that features from ALS and Sentinel-2A data sensitive to forest structure are essential for identifying old-growth forests. Integrating open-access satellite imageries, such as Sentinel-2A and ALS data, can benefit forest managers, stakeholders, and conservationists in monitoring old-growth forest preservation across a broader spatial extent. [ABSTRACT FROM AUTHOR]

Vegetation density extraction is influenced by the characteristics of satellite images, vegetation type, classification algorithm, and region, but there is little information about multispectral imaging (MSI). Studying the compatibility of the information content of sensors to replace sensors in areas where there is no easy access to their data is necessary for remote sensing (RS) studies. This study aims to assess the suitability of MSI from Sentinel-2 and Spot-5 satellites for generating pasture density maps. The Middle Kashkan watershed in the Lorestan Province of Iran was the study area. Geometric correction of the images was performed using ground control points (GCP) and the area's digital elevation model, achieving an accuracy of 0.21 pixels or better. Supervised classification techniques including parallelogram, minimal distance, maximum likelihood, and artificial neural network (ANN) algorithms were applied to the primary MSI of each satellite, as well as the integrated image of Spot-5 and the resulting pasture density map. Three density categories were considered: 5–25%, 25–50%, and over 50%. To validate the accuracy of the classification, a ground truth map of the region was created by interpreting a referenced official digital orthophotomosaic image at a scale of 1:40,000. Comparative analysis of the classified images revealed that the Sentinel-2 image with PCA-2-8 band composition and ANN classification algorithm yielded superior results, with an overall accuracy of 65.72% and a kappa coefficient of 0.08, compared to the Spot-5 image with PCA-3-1 band composition and the ANN classification algorithm. Spot-5 satellite images demonstrated greater effectiveness in generating pasture cover maps across the three density categories. These findings suggest that satellite images with suitable spatial and spectral resolution can be effectively utilized for generating accurate pasture density maps and monitoring long-term pasture preservation, particularly in regions characterized by high aerial photography altitudes in pasture areas. This approach holds the potential for effective pasture management and conservation efforts on a global scale. [ABSTRACT FROM AUTHOR]

Copyright of Remote Sensing for Natural Resources is the property of Remote Sensing for Natural Resources Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study uses a small unmanned aircraft system equipped with a multispectral sensor to assess various vegetation indices (VIs) for their potential to monitor iron deficiency chlorosis (IDC) in a grain sorghum (Sorghum bicolor L.) crop. IDC is a nutritional disorder that stunts a plants' growth and causes its leaves to yellow due to an iron deficit. The objective of this project is to find the best VI to detect and monitor IDC. A series of flights were completed over the course of the growing season and processed using Structure‐from‐Motion photogrammetry to create orthorectified, multispectral reflectance maps in the red, green, red‐edge, and near‐infrared wavelengths. Ground data collection methods were used to analyze stress, chlorophyll levels, and grain yield, correlating them to the multispectral imagery for ground control and precise crop examination. The reflectance maps and soil‐removed reflectance maps were used to calculate 25 VIs whose separability was then calculated using a two‐class distance measure, determining which contained the largest separation between the pixels representing IDC and healthy vegetation. The field‐acquired data were used to conclude which VIs achieved the best results for the dataset as a whole and at each level of IDC (low, moderate, and severe). It was concluded that the MERIS terrestrial chlorophyll index, normalized difference red‐edge, and normalized green (NG) indices achieved the highest amount of separation between plants with IDC and healthy vegetation, with the NG reaching the highest levels of separability for both soil‐included and soil‐removed VIs. Core Ideas: A small unmanned aircraft system was utilized to collect multispectral imagery.Soil background noise was assessed and removed via image thresholding and maximum likelihood classification.A total of 25 vegetation indices were evaluated with and without soil.A two‐class distance measure assessed which vegetation index best detected iron deficiency chlorosis.Normalized green, normalized difference red‐edge, and MERIS terrestrial chlorophyll indices performed best. [ABSTRACT FROM AUTHOR]

Lately, the inclusion of additional lactic acid bacteria (LAB) strains to cheeses is becoming more popular since they can affect cheese's nutritional, technological, and sensory properties, as well as increase the product's safety. This work studied the effect of Lactiplantibacillus pentosus L33 and Lactiplantibacillus plantarum L125 free cells and supernatants on feta cheese quality and Listeria monocytogenes fate. In addition, rapid and non-invasive techniques such as Fourier transform infrared (FTIR) and multispectral imaging (MSI) analysis were used to classify the cheese samples based on their sensory attributes. Slices of feta cheese were contaminated with 3 log CFU/g of L. monocytogenes, and then the cheese slices were sprayed with (i) free cells of the two strains of the lactic acid bacteria (LAB) in co-culture (F, ~5 log CFU/g), (ii) supernatant of the LAB co-culture (S) and control (C, UHT milk) or wrapped with Na-alginate edible films containing the pellet (cells, FF) or the supernatant (SF) of the LAB strains. Subsequently, samples were stored in air, in brine, or in vacuum at 4 and 10 °C. During storage, microbiological counts, pH, and water activity (aw) were monitored while sensory assessment was conducted. Also, in every sampling point, spectral data were acquired by means of FTIR and MSI techniques. Results showed that the initial microbial population of Feta was ca. 7.6 log CFU/g and consisted of LAB (>7 log CFU/g) and yeast molds in lower levels, while no Enterobacteriaceae were detected. During aerobic, brine, and vacuum storage for both temperatures, pathogen population was slightly postponed for S and F samples and reached lower levels compared to the C ones. The yeast mold population was slightly delayed in brine and vacuum packaging. For aerobic storage at 4 °C, an elongation in the shelf life of F samples by 4 days was observed compared to C and S samples. At 10 °C, the shelf life of both F and S samples was extended by 13 days compared to C samples. FTIR and MSI analyses provided reliable estimations of feta quality using the PLS-DA method, with total accuracy (%) ranging from 65.26 to 84.31 and 60.43 to 89.12, respectively. In conclusion, the application of bioprotective LAB strains can result in the extension of feta's shelf life and provide a mild antimicrobial action against L. monocytogenes and spoilage microbiota. Furthermore, the findings of this study validate the effectiveness of FTIR and MSI techniques, in tandem with data analytics, for the rapid assessment of the quality of feta samples. [ABSTRACT FROM AUTHOR]

The short eleventh-century lyric Hebban olla vogala is considered the earliest literary text in Dutch. Yet it only survives as a badly faded pen trial, written in England by a monk from the Low Countries. As a result, the exact reading of the text and even the language it was written in remain uncertain. Now, multispectral imaging of the manuscript has made it possible to provide an improved reconstruction of the text. An analysis of this new reading suggests that the scribe deliberately used the similarity between dialects of late Old English and Old Dutch to produce a rhyming verse text that was intelligible in both languages. As a multilingual and translingual poem, Hebban olla vogala must therefore be situated in English as well as Dutch literary history. The scribe's other pen trials in the same manuscript demonstrate an interest in different Latin verse forms, indicating that Hebban olla vogala may also be a literary experiment. In exploiting the mutual intelligibility of Old Dutch and Old English, the poem points to an easily overlooked current of influence on late Old English and early Middle English literature. While this is hard to trace due to the similarities between the two languages, Dutch emerges as another potential influence, alongside Anglo-Norman French, on the development of rhyming verse in English after the Norman Conquest. In this way, Hebban olla vogala can be seen not only as a monument of Dutch literary history, but also as a testament to its interaction with English literature. [ABSTRACT FROM AUTHOR]

Traditional soil salinity measurement methods are generally complex and labor-intensive, restricting the long-term monitoring of soil salinity, particularly in arid areas. In this context, the soil salt content (SSC) data from farms in the Heihe River Basin in Northwest China were collected in three consecutive years (2021, 2022, and 2023). In addition, the spectral reflectance and texture features of different sampling sites in the study area were extracted from long-term unmanned aerial vehicle (UAV) multispectral images to replace the red and near-infrared bands with a newly introduced red edge band. The spectral index was calculated in this study before using four sensitive variable combinations to predict soil salt contents. A Pearson correlation analysis was performed in this study to screen 57 sensitive features. In addition, 36 modeling scenarios were conducted based on the Extreme Gradient Boosting (XGBoost Implemented using R language 4.3.1), Backpropagation Neural Network (BPNN), and Random Forest (RF) algorithms. The most optimal algorithms for predicting the soil salt contents in farmland located in the Heihe River Basin, in the arid region of Northwest China, were determined. The results showed a higher prediction accuracy for the XGBoost algorithm than the RF and BPNN algorithms, accurately reflecting the actual soil salt contents in the arid area. On the other hand, the most accurate predicted soil salt contents were obtained in 2023 using the XGBoost algorithm, with coefficient of determination (R2), root mean square error (RMSE), and mean absolute error (MAE) ranges of 0.622–0.820, 0.086–0.157, and 0.078–0.134, respectively, whereas the most stable prediction results were obtained using the collected data in 2022. From the perspective of different sensitive variable input combinations, the implementation of the XGBoost algorithm using the spectral index–spectral reflectance–texture feature input combination resulted in comparatively higher prediction accuracies than those of the other variable combinations in 2022 and 2023. Specifically, the R2, RMSE, and MAE values obtained using the spectral index–spectral reflectance–texture feature input combination were 0.674, 0.133, and 0.086 in 2022 and 0.820, 0.165, and 0.134 in 2023, respectively. Therefore, our results demonstrated that the spectral index–spectral reflectance–texture feature was the optimal sensitive variable input combination for the machine learning algorithms, of which the XGBoost algorithm is the most optimal model for predicting soil salt contents. The results of this study provide a theoretical basis for the rapid and accurate prediction of soil salinity in arid areas. [ABSTRACT FROM AUTHOR]

In response to the limitations of meteorological conditions in global rice growing areas and the high cost of annotating samples, this paper combines the Vertical-Vertical (VV) polarization and Vertical-Horizontal (VH) polarization backscatter features extracted from Sentinel-1 synthetic aperture radar (SAR) images and the NDVI, NDWI, and NDSI spectral index features extracted from Sentinel-2 multispectral images. By leveraging the advantages of an optimized Semi-Supervised Generative Adversarial Network (optimized SSGAN) in combining supervised learning and semi-supervised learning, rice extraction can be achieved with fewer annotated image samples. Within the optimized SSGAN framework, we introduce a focal-adversarial loss function to enhance the learning process for challenging samples; the generator module employs the Deeplabv3+ architecture, utilizing a Wide-ResNet network as its backbone while incorporating dropout layers and dilated convolutions to improve the receptive field and operational efficiency. Experimental results indicate that the optimized SSGAN, particularly when utilizing a 3/4 labeled sample ratio, significantly improves rice extraction accuracy, leading to a 5.39% increase in Mean Intersection over Union (MIoU) and a 2.05% increase in Overall Accuracy (OA) compared to the highest accuracy achieved before optimization. Moreover, the integration of SAR and multispectral data results in an OA of 93.29% and an MIoU of 82.10%, surpassing the performance of single-source data. These findings provide valuable insights for the extraction of rice information in global rice-growing regions. [ABSTRACT FROM AUTHOR]

Rice blast disease is one of the major diseases affecting rice plant, significantly impacting both yield and quality. Current detecting methods for rice blast disease mainly rely on manual surveys in the field and laboratory tests, which are inefficient, inaccurate, and limited in scale. Spectral and imaging technologies in the visible and near-infrared (Vis/NIR) region have been widely investigated for crop disease detection. This work explored the potential of integrating canopy reflectance spectra acquired near the ground and aerial multispectral images captured with an unmanned aerial vehicle (UAV) for estimating Soil-Plant Analysis Development (SPAD) values and detecting rice leaf blast disease in the field. Canopy reflectance spectra were preprocessed, followed by effective band selection. Different vegetation indices (VIs) were calculated from multispectral images and selected for model establishment according to their correlation with SPAD values and disease severity. The full-wavelength canopy spectra (450–850 nm) were first used for establishing SPAD inversion and blast disease classification models, demonstrating the effectiveness of Vis/NIR spectroscopy for SPAD inversion and blast disease detection. Then, selected effective bands from the canopy spectra, UAV VIs, and the fusion of the two data sources were used for establishing corresponding models. The results showed that all SPAD inversion models and disease classification models established with the integrated data performed better than corresponding models established with the single of either of the aerial and ground data sources. For SPAD inversion models, the best model based on a single data source achieved a validation determination coefficient ( R c v 2 ) of 0.5719 and a validation root mean square error (RMSECV) of 2.8794, while after ground and aerial data fusion, these two values improved to 0.6476 and 2.6207, respectively. For blast disease classification models, the best model based on a single data source achieved an overall test accuracy of 89.01% and a Kappa coefficient of 0.86, and after data fusion, the two values improved to 96.37% and 0.95, respectively. These results indicated the significant potential of integrating canopy reflectance spectra and UAV multispectral images for detecting rice diseases in large fields. [ABSTRACT FROM AUTHOR]

In order to improve the accuracy of multispectral image inversion of soil and plant analytical development (SPAD) of the cotton canopy, image segmentation methods were utilized to remove the background interference, such as soil and shadow in UAV multispectral images. UAV multispectral images of cotton bud stage canopies at three different heights (30 m, 50 m, and 80 m) were acquired. Four methods, namely vegetation index thresholding (VIT), supervised classification by support vector machine (SVM), spectral mixture analysis (SMA), and multiple endmember spectral mixture analysis (MESMA), were used to segment cotton, soil, and shadows in the multispectral images of cotton. The segmented UAV multispectral images were used to extract the spectral information of the cotton canopy, and eight vegetation indices were calculated to construct the dataset. Partial least squares regression (PLSR), Random forest (FR), and support vector regression (SVR) algorithms were used to construct the inversion model of cotton SPAD. This study analyzed the effects of different image segmentation methods on the extraction accuracy of spectral information and the accuracy of SPAD modeling in the cotton canopy. The results showed that (1) The accuracy of spectral information extraction can be improved by removing background interference such as soil and shadows using four image segmentation methods. The correlation between the vegetation indices calculated from MESMA segmented images and the SPAD of the cotton canopy was improved the most; (2) At three different flight altitudes, the vegetation indices calculated by the MESMA segmentation method were used as the input variable, and the SVR model had the best accuracy in the inversion of cotton SPAD, with R2 of 0.810, 0.778, and 0.697, respectively; (3) At a flight altitude of 80 m, the R2 of the SVR models constructed using vegetation indices calculated from images segmented by VIT, SVM, SMA, and MESMA methods were improved by 2.2%, 5.8%, 13.7%, and 17.9%, respectively, compared to the original images. Therefore, the MESMA mixed pixel decomposition method can effectively remove soil and shadows in multispectral images, especially to provide a reference for improving the inversion accuracy of crop physiological parameters in low-resolution images with more mixed pixels. [ABSTRACT FROM AUTHOR]

The assessment of the transformation zone is a critical step toward diagnosis of cervical cancer. This work involves the development of a portable, label‐free transvaginal multispectral diffuse optical imaging (MDOI) imaging probe to estimate the transformation zone. The images were acquired from N = 5 (N = 1 normal, N = 2 premalignant, and N = 2 malignant) patients. Key parameters such as spectral contrast ratio (ρ) at 545 and 450 nm were higher in premalignant (0.29, 0.25 for 450 nm and 0.30, 0.17 for 545 nm) as compared to the normal patients (0.13 and 0.14 for 450 and 545 nm, respectively). The threshold for the spectral intensity ratio R610/R450 and R610/R545 can also be used as a marker to correlate with the new and original squamous columnar junction (SCJ), respectively. The pilot study highlights the use of new markers such as spectral contrast ratio (ρ) and spectral intensity ratio (R610/R450 and R610/R545) images. [ABSTRACT FROM AUTHOR]

The text is a review of Kilian Fleischer's book "Philodem, Geschichte der Akademie: Einfuhrung, Ausgabe, Kommentar," which focuses on the Herculaneum Papyri and the techniques used to study them. The book provides new insights into the history of the Academy and covers various topics, including the death and burial of Plato. The reviewer praises the book's usefulness for researchers and its well-organized content. The text also discusses the reconstruction and interpretation of a fragment of a philosophical text, possibly written by Plato, exploring alternative readings and interpretations. Overall, the article highlights the significance of the papyrus fragment for understanding Plato and his school. [Extracted from the article]

Abstract Background Wheat (Triticum aestivum L.) is an important grain crops in the world, and its growth and development in different stages is seriously affected by saline-alkali stress, especially in seedling stage. Therefore, nondestructive detection of wheat seedlings under saline-alkali stress can provide more comprehensive technical support for wheat breeding, cultivation and management. Results This research focused on moisture signal prediction and classification of saline-alkali stress in wheat seedlings using fusion techniques. After collecting and analyzing transverse relaxation time and Multispectral imaging (MSI) information of wheat seedlings, four regression models were used to predict the moisture signal. K-Nearest Neighbor (KNN) and Gaussian-Naïve Bayes (GNB) models were combined with fivefold cross validation to classify the prediction of wheat seedling stress. The results showed that wheat seedlings would increase the bound water content through a certain mechanism to enhance their saline-alkali stress. Under the same Na concentration, the effect of alkali stress on moisture, growth and spectrum of wheat seedlings is stronger than salt stress. The Gradient Boosting Decision Regression Tree model performs the best in predicting wheat moisture signals, with a coefficient of determination (R2P) of 0.98 and a root mean square error of 109.60. It also had a short training time (1.48 s) and an efficient prediction speed (1300 obs/s). The KNN and GNB demonstrated significantly enhanced predictive performance when classifying the fused dataset, compared to using single datasets individually. In particular, the GNB model performing best on the fused dataset, with Precision, Recall, Accuracy, and F1-score of 90.30, 88.89%, 88.90%, and 0.90, respectively. Conclusions Under the same Na concentration, the effects of alkali stress on water content, spectrum, and growth of wheat were stronger than that of salt stress, which was more unfavorable to the growth of wheat. The fusion of low-field nuclear magnetic resonance and MSI technology can improve the classification of wheat stress, and provide an effective technical method for rapid and accurate monitoring of wheat seedlings under saline-alkali stress. Graphical Abstract

BACKGROUND: Phyllanthus emblica Linn. (PE) is rich in polyphenols, which can be categorized into free and bound phenolics (PEFP and PEBP). This study evaluated the inhibitory effect of PEFB and PEBP on α‐amylase for the first time. The mechanism of the inhibition effect of PEFP and PEBP on α‐amylase was investigated by enzyme inhibition kinetics, multispectral analysis, thermodynamics, and molecular docking. RESULTS: Free and bound phenolics inhibited α‐amylase activity effectively in a mixed type of inhibition. Fluorescence quenching and thermodynamic analyses showed that the binding of PEFP and PEBP to α‐amylase occurred through a static quenching process (Kq = 6.94 × 10¹² and 5.74 × 10¹² L mol−1 s−1), which was accompanied by a redshift (λem from 343 to 347 nm), leading to a change in the microenvironment. This process was found to be a spontaneous exothermic reaction (ΔG < 0). Circular dichroism (CD) analysis confirms that the secondary structure of α‐amylase was altered, in particular a decrease in α‐helixes and an increase in random coils. Molecular docking studies showed that PEFP and PEBP interacted with α‐amylase through hydrogen bonding and hydrophobic interactions. CONCLUSION: The present study provides valuable insights into the mechanism of action of PEFP and PEBP on α‐amylase, which will provide a theoretical basis for their possible use as novel natural α‐amylase inhibitors. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Abstract With rising melanoma incidence and mortality, early detection and surgical removal of primary lesions is essential. Multispectral imaging is a new, non-invasive technique that can facilitate skin cancer detection by measuring the reflectance spectra of biological tissues. Currently, incident illumination allows little light to be reflected from deeper skin layers due to high surface reflectance. A pilot study was conducted at the University Hospital Basel to evaluate, whether multispectral imaging with direct light coupling could extract more information from deeper skin layers for more accurate dignity classification of melanocytic lesions. 27 suspicious pigmented lesions from 23 patients were included (6 melanomas, 6 dysplastic nevi, 12 melanocytic nevi, 3 other). Lesions were imaged before excision using a prototype snapshot mosaic multispectral camera with incident and direct illumination with subsequent dignity classification by a pre-trained multispectral image analysis model. Using incident light, a sensitivity of 83.3% and a specificity of 58.8% were achieved compared to dignity as determined by histopathological examination. Direct light coupling resulted in a superior sensitivity of 100% and specificity of 82.4%. Convolutional neural network classification of corresponding red, green, and blue lesion images resulted in 16.7% lower sensitivity (83.3%, 5/6 malignant lesions detected) and 20.9% lower specificity (61.5%) compared to direct light coupling with multispectral image classification. Our results show that incorporating direct light multispectral imaging into the melanoma detection process could potentially increase the accuracy of dignity classification. This newly evaluated illumination method could improve multispectral applications in skin cancer detection. Further larger studies are needed to validate the camera prototype.

Abstract Vegetation density extraction is influenced by the characteristics of satellite images, vegetation type, classification algorithm, and region, but there is little information about multispectral imaging (MSI). Studying the compatibility of the information content of sensors to replace sensors in areas where there is no easy access to their data is necessary for remote sensing (RS) studies. This study aims to assess the suitability of MSI from Sentinel-2 and Spot-5 satellites for generating pasture density maps. The Middle Kashkan watershed in the Lorestan Province of Iran was the study area. Geometric correction of the images was performed using ground control points (GCP) and the area's digital elevation model, achieving an accuracy of 0.21 pixels or better. Supervised classification techniques including parallelogram, minimal distance, maximum likelihood, and artificial neural network (ANN) algorithms were applied to the primary MSI of each satellite, as well as the integrated image of Spot-5 and the resulting pasture density map. Three density categories were considered: 5–25%, 25–50%, and over 50%. To validate the accuracy of the classification, a ground truth map of the region was created by interpreting a referenced official digital orthophotomosaic image at a scale of 1:40,000. Comparative analysis of the classified images revealed that the Sentinel-2 image with PCA-2-8 band composition and ANN classification algorithm yielded superior results, with an overall accuracy of 65.72% and a kappa coefficient of 0.08, compared to the Spot-5 image with PCA-3-1 band composition and the ANN classification algorithm. Spot-5 satellite images demonstrated greater effectiveness in generating pasture cover maps across the three density categories. These findings suggest that satellite images with suitable spatial and spectral resolution can be effectively utilized for generating accurate pasture density maps and monitoring long-term pasture preservation, particularly in regions characterized by high aerial photography altitudes in pasture areas. This approach holds the potential for effective pasture management and conservation efforts on a global scale.

Abstract Background Pepper Phytophthora blight is a devastating disease during the growth process of peppers, significantly affecting their yield and quality. Accurate, rapid, and non-destructive early detection of pepper Phytophthora blight is of great importance for pepper production management. This study investigated the possibility of using multispectral imaging combined with machine learning to detect Phytophthora blight in peppers. Peppers were divided into two groups: one group was inoculated with Phytophthora blight, and the other was left untreated as a control. Multispectral images were collected at 0-h samples before inoculation and at 48, 60, 72, and 84 h after inoculation. The supporting software of the multispectral imaging system was used to extract spectral features from 19 wavelengths, and textural features were extracted using a gray-level co-occurrence matrix (GLCM) and a local binary pattern (LBP). The principal component analysis (PCA), successive projection algorithm (SPA), and genetic algorithm (GA) were used for feature selection from the extracted spectral and textural features. Two classification models were established based on effective single spectral features and significant spectral textural fusion features: a partial least squares discriminant analysis (PLS_DA) and one-dimensional convolutional neural network (1D-CNN). A two-dimensional convolutional neural network (2D-CNN) was constructed based on five principal component (PC) coefficients extracted from the spectral data using PCA, weighted, and summed with 19-channel multispectral images to create new PC images. Results The results indicated that the models using PCA for feature selection exhibit relatively stable classification performance. The accuracy of PLS-DA and 1D-CNN based on single spectral features is 82.6% and 83.3%, respectively, at the 48h mark. In contrast, the accuracy of PLS-DA and 1D-CNN based on spectral texture fusion reached 85.9% and 91.3%, respectively, at the same 48h mark. The accuracy of the 2D-CNN based on 5 PC images is 82%. Conclusions The research indicates that Phytophthora blight infection can be detected 48 h after inoculation (36 h before visible symptoms). This study provides an effective method for the early detection of Phytophthora blight in peppers.

The pansharpening method based on spatial injection is prone to spectral distortion due to the spatial detail injection amount mismatch. For this reason, this paper proposes a method that combines adaptive intensity component extraction and spatial detail injection optimisation. Firstly, a new method for extracting the I component of multispectral (MS) image is proposed, which decomposes each band of MS image and panchromatic (PAN) image according to the structure-energy mechanism through the joint bilateral filter and then determines the weight coefficient of each band of MS image to extract the I component by solving the minimum optimisation problem of the structure layer of PAN image and the energy layer of MS image. Secondly, a spatial detail extraction method combining local spatial frequency and local energy is proposed, and the method can fully extract the fine detail features of PAN images and MS images. In this paper, many experiments are conducted on QuickBird, GeoEye-1 and WorldView-4 datasets, and quantitative and qualitative comparisons are made with eight advanced methods, and qualitative and quantitative analyses of the experimental results show that the proposed method has certain superiority. [ABSTRACT FROM AUTHOR]

Background: Early detection and treatment are effective methods for the management of oral squamous cell carcinoma (OSCC), which can be facilitated by the detection of tumor-specific OSCC biomarkers. The epidermal growth factor receptor (EGFR) and programmed death-ligand 1 (PD-L1) are important therapeutic targets for OSCC. Multispectral fluorescence molecular imaging (FMI) can facilitate the detection of tumor multitarget expression with high sensitivity and safety. Hence, we developed Nimotuzumab-ICG and Atezolizumab-Cy5.5 imaging probes, in combination with multispectral FMI, to sensitively and noninvasively identify EGFR and PD-L1 expression for the detection and comprehensive treatment of OSCC. Methods: The expression of EGFR and PD-L1 was analyzed using bioinformatics data sources and specimens. Nimotuzumab-ICG and Atezolizumab-Cy5.5 imaging probes were developed and tested on preclinical OSCC cell line and orthotopic OSCC mouse model, fresh OSCC patients' biopsied samples, and further clinical mouthwash trials were conducted in OSCC patients. Results: EGFR and PD-L1 were specifically expressed in human OSCC cell lines and tumor xenografts. Nimotuzumab-ICG and Atezolizumab-Cy5.5 imaging probes can specifically target to the tumor sites in an in situ human OSCC mouse model with good safety. The detection sensitivity and specificity of Nimotuzumab-ICG in patients were 96.4% and 100%, and 95.2% and 88.9% for Atezolizumab-Cy5.5. Conclusions: EGFR and PD-L1 are highly expressed in OSCC, the combination of which is important for a precise prognosis of OSCC. EGFR and PD-L1 expression can be sensitively detected using the newly synthesized multispectral fluorescence imaging probes Nimotuzumab-ICG and Atezolizumab-Cy5.5, which can facilitate the sensitive and specific detection of OSCC and improve treatment outcomes. Trial registration: Chinese Clinical Trial Registry, ChiCTR2100045738. Registered 23 April 2021, https://www.chictr.org.cn/bin/project/edit?pid=125220 [ABSTRACT FROM AUTHOR]

One of the finest fifteenth-century portraits of the Burgundian Duke Philip the Good resides in the Musée des Beaux-Arts in Dijon. This small yet exceptionally crafted panel holds significance for both historians and art historians alike. Surprisingly, prior to this study, the context, the dating and the authorship of the piece remained obscure, and the widely circulated hypothesis of it being "a copy of a lost portrait by Rogier van der Weyden" has never been corroborated by convincing arguments. Clarifying the context, dating and authorship of the painting were the primary objectives of the investigations discussed in the article. Therefore, this painting underwent a multidisciplinary investigation spanning both the positive and human sciences. Macro-XRF scans were conducted alongside hyperspectral reflectance scans, multispectral imaging in the visible and infrared range, and optical coherence tomography. These analyses were complemented by an art historical study. As a result, a precise delineation between authentic and retouched sections was achieved. This article does not merely present the various perspectives separately but constructs a coherent narrative based on all these foundations. This holistic multidisciplinary research methodology produced a clear account, albeit with some scope for future inquiry. The involvement of the painter Pieter Cristus was conclusively demonstrated. This painter, whether himself personally, an assistant in the workshop or a contemporary follower, is attributed to the genesis of this work, which is presumed to be not the original portrait but a contemporaneous copy, possibly commissioned by the Burgundian Duke himself. While we no longer remain in the dark and have lifted some veils, this study also paves the way for further investigation into this panel and the numerous other portraits of Philip the Good. [ABSTRACT FROM AUTHOR]

This study explores the dynamic evolution of the Hunga Volcano in the Kingdom of Tonga and covers two volcanic unrests, focusing on the creation and subsequent disappearance of a new island between Hunga Tonga (HT) and Hunga Ha'apai (HH) between 2013 and 2023. The island expanded in 2015 and vanished in January 2022 due to a massive eruption (VEI = 6), featuring a 57 km high volcanic cloud and generating multiple tsunamis that caused damage across the Pacific Ocean. Utilizing remote sensing techniques, including multispectral imaging from Sentinel 2, Landsat 8-9, and synthetic aperture radar (SAR) imaging from Sentinel 1, the research employs a supervised random forest classification algorithm to individuate the changing subaerial surface area of the volcano. This approach documents size variations in the islands, particularly during weeks surrounding two volcanic unrests. The classifier, trained on nearly cloud-free multispectral images, automatically delineates surface area changes over the years. The temporal resolution of area change, limited to images with less than 5% cloudiness, encompasses about 50% of Landsat and 20% of Sentinel 2 images between 2013 and 2023, selected from 739 available images. The multispectral observations are complemented by 215 Sentinel 1 SAR images, penetrating clouds, though with limited bands. Despite higher noise, the classifier on Sentinel 1 successfully distinguishes land from ocean. Sentinel 1 observations, starting in 2014, cover the volcano unrest of 2014/2015. Earth Engine, a cloud computing data facility, is used for processing. Analysis indicates a slight decrease in the area change post-2015 island formation and identifies the disappearance of the island bridge connecting HT and HH, along with two smaller islands south of HT and HH in 2022. The 2022 explosion is preceded by an increase in island area in the weeks before the eruption. Global satellite coverage could automatically detect changes in oceanic areas and distinguish water from new volcanic islands, offering a means of identifying volcanic unrests and documenting their evolution. [ABSTRACT FROM AUTHOR]

Introduction: Myeloid sarcomas (MS) comprise rare extramedullary manifestations of myeloid neoplasms with poor patients' outcome. While the clinical relevance of the tumor microenvironment (TME) is well established in many malignancies, there exists limited information in MS. Methods: The expression of the human leukocyte antigen class I (HLA-I) antigens, HLA-I antigen processing and presenting machinery (APM) components and the composition of the TME of 45 MS and paired bone marrow (BM) samples from two independent cohorts were assessed by immunohistochemistry, multispectral imaging, and RNA sequencing (RNAseq). Results: A significant downregulation of the HLA-I heavy chain (HC; 67.5%) and ß2-microglobulin (ß2M; 64.8%), but an upregulation of HLA-G was found in MS compared to BM samples, which was confirmed in a publicly available dataset. Moreover, MS tumors showed a predominantly immune cell excluded TME with decreased numbers of tissue infiltrating lymphocytes (TILs) (9.5%) compared to paired BM (22.9%). RNAseq analysis of a subset of 10 MS patients with preserved and reduced HLA-I HC expression revealed 150 differentially expressed genes and a significantly reduced expression of inflammatory response genes was found in samples with preserved HLA-I expression. Furthermore, low HLA-I expression and low TIL numbers in the TME of MS cases were linked to an inferior patients' outcome. Discussion: This study demonstrated a high prevalence of immune escape strategies in the pathogenesis and extramedullary spread of MS, which was also found in patients without evidence of any BM pathology, which yields the rational for the development of novel individually tailored therapies for MS patients. [ABSTRACT FROM AUTHOR]

Assessing ecosystem health on a large scale is crucial for a wide range of management and regulatory decisions. Technologies such as hyperspectral imaging allow noninvasive and rapid estimation of key attributes based on observed reflectance. However, these images are high-dimensional and real-world applications require models based on fewer wavelengths. This paper proposes a new wavelength selection and feature extraction method for hyperspectral image analysis based on genetic programming to automatically select key wavelength regions and informative image features. A dataset of hyperspectral images of sediment in the field was collected and paired with ground-truth measurements of the sediment porosity and organic matter content. Two new program structures were proposed to construct feature extraction trees from either the mean reflectance spectra (spectra-based) or full hyperspectral images (image-based). SVR models were constructed to predict attributes based on the extracted features. Various regression models were used to predict the porosity and organic matter content. Full-wavelength models were constructed to reliably predict the organic matter content. The proposed spectra-based genetic programming solutions show competitive results compared to common wavelength selection methods, such as SPA, CARS, and RC. Finally, the best-evolved solution was applied to predict sediment organic matter content across all collected images. [ABSTRACT FROM AUTHOR]

The complex interplay between hydrogen peroxide (H 2 O 2) and nitric oxide (NO) in endothelial cells presents challenges due to technical limitations in simultaneous measurement, hindering the elucidation of their direct relationship. Previous studies have yielded conflicting findings regarding the impact of H 2 O 2 on NO production. To address this problem, we employed genetically encoded biosensors, HyPer7 for H 2 O 2 and geNOps for NO, allowing simultaneous imaging in single endothelial cells. Optimization strategies were implemented to enhance biosensor performance, including camera binning, temperature regulation, and environmental adjustments to mimic physiological normoxia. Our results demonstrate that under ambient oxygen conditions, H 2 O 2 exhibited no significant influence on NO production. Subsequent exploration under physiological normoxia (5 kPa O 2) revealed distinct oxidative stress levels characterized by reduced basal HyPer7 signals, enhanced H 2 O 2 scavenging kinetics, and altered responses to pharmacological treatment. Investigation of the relationship between H 2 O 2 and NO under varying oxygen conditions revealed a lack of NO response to H 2 O 2 under hyperoxia (18 kPa O 2) but a modest NO response under physiological normoxia (5 kPa O 2). Importantly, the NO response was attenuated by l -NAME, suggesting activation of eNOS by endogenous H 2 O 2 generation upon auranofin treatment. Our study highlights the intricate interplay between H 2 O 2 and NO within the endothelial EA.hy926 cell line, emphasizing the necessity for additional research within physiological contexts due to differential response observed under physiological normoxia (5 kPa O 2). This further investigation is essential for a comprehensive understanding of the H 2 O 2 and NO signaling considering the physiological effects of ambient O 2 levels involved. [Display omitted] • Co-expressing HyPer7 and O-geNOps enable multichromatic imaging of H 2 O 2 and NO in endothelial cells. • Exogenous H 2 O 2 does not affect NO levels under hyperoxic and normoxic conditions. • Endogenous H 2 O 2 generation has minimal NO impact under normoxic conditions. [ABSTRACT FROM AUTHOR]

Studying brain‐wide hemodynamic responses to different stimuli at high spatiotemporal resolutions can help gain new insights into the mechanisms of neuro‐ diseases and ‐disorders. Nonetheless, this task is challenging, primarily due to the complexity of neurovascular coupling, which encompasses interdependent hemodynamic parameters including cerebral blood volume (CBV), cerebral blood flow (CBF), and cerebral oxygen saturation (SO2). The current brain imaging technologies exhibit inherent limitations in resolution, sensitivity, and imaging depth, restricting their capacity to comprehensively capture the intricacies of cerebral functions. To address this, a multimodal functional ultrasound and photoacoustic (fUSPA) imaging platform is reported, which integrates ultrafast ultrasound and multispectral photoacoustic imaging methods in a compact head‐mountable device, to quantitatively map individual dynamics of CBV, CBF, and SO2 as well as contrast agent enhanced brain imaging at high spatiotemporal resolutions. Following systematic characterization, the fUSPA system is applied to study brain‐wide cerebrovascular reactivity (CVR) at single‐vessel resolution via relative changes in CBV, CBF, and SO2 in response to hypercapnia stimulation. These results show that cortical veins and arteries exhibit differences in CVR in the stimulated state and consistent anti‐correlation in CBV oscillations during the resting state, demonstrating the multiparametric fUSPA system's unique capabilities in investigating complex mechanisms of brain functions. [ABSTRACT FROM AUTHOR]

Introduction: This research explores the application of generative artificial intelligence, specifically the novel ARISGAN framework, for generating highresolution synthetic satellite imagery in the challenging arctic environment. Realistic and high-resolution surface imagery in the Arctic is crucial for applications ranging from satellite retrieval systems to the wellbeing and safety of Inuit populations relying on detailed surface observations. Methods: The ARISGAN framework was designed by combining dense block, multireceptive field, and Pix2Pix architecture. This innovative combination aims to address the need for high-quality imagery and improve upon existing state-of-the-art models. Various tasks and metrics were employed to evaluate the performance of ARISGAN, with particular attention to land-based and sea icebased imagery. Results: The results demonstrate that the ARISGAN framework surpasses existing state-of-the-art models across diverse tasks and metrics. Specifically, land-based imagery super-resolution exhibits superior metrics compared to sea ice-based imagery when evaluated across multiple models. These findings confirm the ARISGAN framework's effectiveness in generating perceptually valid highresolution arctic surface imagery. Discussion: This study contributes to the advancement of Earth Observation in polar regions by introducing a framework that combines advanced image processing techniques with a well-designed architecture. The ARISGAN framework's ability to outperform existing models underscores its potential. Identified limitations include challenges in temporal synchronicity, multispectral image analysis, preprocessing, and quality metrics. The discussion also highlights potential avenues for future research, encouraging further refinement of the ARISGAN framework to enhance the quality and availability of high-resolution satellite imagery in the Arctic. [ABSTRACT FROM AUTHOR]

This study aimed at estimating the macro- and micronutrient concentrations in leaves of Valencia oranges (Citrus sinensis [L.] Osbeck) through multiple regression analysis using multispectral images and vegetation indices (VIs) selected through the stepwise regression method. The data were collected in a commercial orchard of Valencia oranges in El Barretal, Tamaulipas, Mexico. The leaf samples were analysed in a laboratory to determine the concentrations of N, P, K, Ca, Mg, Cu, Fe, Mn, and Zn using traditional chemical methods; the relative chlorophyll values were obtained using a chlorophyll metre (SPAD502 Plus). Multi-spectral images of the tree canopy were acquired using a UAV equipped with a multispectral camera with five wavelengths (blue, green, red, red edge, and near-infrared); based on these data, 85 VIs were calculated. To select the predictor variables that were most relevant for the model, 90 candidate variables were divided into three groups with 30 VIs grouped by nutrients based on the highest and lowest absolute values found in the correlation matrix. Heteroscedasticity and autocorrelation were also assessed through the Breusch-Pagan and Durbin-Watson tests, respectively. The selected variables were used in multiple regression analysis. The resulting models were validated by mean absolute error (MAE), root-mean-square error (RMSE), mean absolute percentage error (MAPE), and coefficient of determination (${R^2}$ R 2 ). The prediction models showed satisfactory results, with values ${R^2}$ R 2 between 0.79 and 0.95 and relatively low (<10) MAE and RMSE for all nutrients, except Fe ($ \lt 30$ < 30) in all phenological stages; MAPE values were lower than 0.5% for all nutrients. This study showed that multispectral imaging combined with multiple regression analysis effectively predicts nutrient levels in the leaves of Valencia oranges in various phenological stages. This study presents a new perspective on agricultural systems, offering an effective alternative to assess the nutritional conditions of crops. [ABSTRACT FROM AUTHOR]

Fire blight is an infectious disease found in apple and pear orchards. While managing the disease is critical to maintaining orchard health, identifying symptoms early is a challenging task which requires trained expert personnel. This paper presents an inspection technique that targets individual symptoms via deep learning and density estimation. We evaluate the effects of including multi-spectral sensors in the model's pipeline. Results show that adding near infrared (NIR) channels can help improve prediction performance and that density estimation can detect possible symptoms when severity is in the mid-high range. [ABSTRACT FROM AUTHOR]

(1) Background: Yield-monitoring systems are widely used in grain crops but are less advanced for hay and forage. Current commercial systems are generally limited to weighing individual bales, limiting the spatial resolution of maps of hay yield. This study evaluated an Uncrewed Aerial Vehicle (UAV)-based imaging system to estimate hay yield. (2) Methods: Data were collected from three 0.4 ha plots and a 35 ha hay field of red clover and timothy grass in September 2020. A multispectral camera on the UAV captured images at 30 m (20 mm pixel−1) and 50 m (35 mm pixel−1) heights. Eleven Vegetation Indices (VIs) and five texture features were calculated from the images to estimate biomass yield. Multivariate regression models (VIs and texture features vs. biomass) were evaluated. (3) Results: Model R2 values ranged from 0.31 to 0.68. (4) Conclusions: Despite strong correlations between standard VIs and biomass, challenges such as variable image resolution and clarity affected accuracy. Further research is needed before UAV-based yield estimation can provide accurate, high-resolution hay yield maps. [ABSTRACT FROM AUTHOR]

Spectral imaging has many applications, from methane detection using satellites to disease detection on crops. However, spectral cameras remain a costly solution ranging from 10 thousand to 100 thousand euros for the hardware alone. Here, we present a low-cost multispectral camera (LC-MSC) with 64 LEDs in eight different colors and a monochrome camera with a hardware cost of 340 euros. Our prototype reproduces spectra accurately when compared to a reference spectrometer to within the spectral width of the LEDs used and the ±1 σ variation over the surface of ceramic reference tiles. The mean absolute difference in reflectance is an overestimate of 0.03 for the LC-MSC as compared to a spectrometer, due to the spectral shape of the tiles. In environmental light levels of 0.5 W m−2 (bright artificial indoor lighting) our approach shows an increase in noise, but still faithfully reproduces discrete reflectance spectra over 400 nm–1000 nm. Our approach is limited in its application by LED bandwidth and availability of specific LED wavelengths. However, unlike with conventional spectral cameras, the pixel pitch of the camera itself is not limited, providing higher image resolution than typical high-end multi- and hyperspectral cameras. For sample conditions where LED illumination bands provide suitable spectral information, our LC-MSC is an interesting low-cost alternative approach to spectral imaging. [ABSTRACT FROM AUTHOR]

Accurately extracting large-scale offshore floating raft aquaculture (FRA) areas is crucial for supporting scientific planning and precise aquaculture management. While remote sensing technology offers advantages such as wide coverage, rapid imaging, and multispectral capabilities for FRA monitoring, the current methods face challenges in terms of establishing spatial–spectral correlations and extracting multiscale features, thereby limiting their accuracy. To address these issues, we propose an innovative multiscale spatial–spectral fusion network (MSSFNet) designed specifically for extracting offshore FRA areas from multispectral remote sensing imagery. MSSFNet effectively integrates spectral and spatial information through a spatial–spectral feature extraction block (SSFEB), significantly enhancing the accuracy of FRA area identification. Additionally, a multiscale spatial attention block (MSAB) captures contextual information across different scales, improving the ability to detect FRA areas of varying sizes and shapes while minimizing edge artifacts. We created the CHN-YE7-FRA dataset using Sentinel-2 multispectral remote sensing imagery and conducted extensive evaluations. The results showed that MSSFNet achieved impressive metrics: an F1 score of 90.76%, an intersection over union (IoU) of 83.08%, and a kappa coefficient of 89.75%, surpassing those of state-of-the-art methods. The ablation results confirmed that the SSFEB and MSAB modules effectively enhanced the FRA extraction accuracy. Furthermore, the successful practical applications of MSSFNet validated its generalizability and robustness across diverse marine environments. These findings highlight the performance of MSSFNet in both experimental and real-world scenarios, providing reliable, precise FRA area monitoring. This capability provides crucial data for scientific planning and environmental protection purposes in coastal aquaculture zones. [ABSTRACT FROM AUTHOR]

A snapshot multi-wavelength birefringence imaging measurement method was proposed in this study. The RGB-LEDs at wavelengths 463 nm, 533 nm, and 629 nm were illuminated with circularly polarized light after passing through a circular polarizer. The transmitted light through the birefringent sample was captured by a color polarization camera. A single imaging process captured light intensity in four polarization directions (0°, 45°, 90°, and 135°) for each of the three RGB spectral wavelength channels, and subsequently measured the first three elements of Stokes vectors (S0, S1, and S2) after the sample. The birefringence retardance and fast-axis azimuthal angle were determined simultaneously. An experimental setup was constructed, and polarization response matrices were calibrated for each spectral wavelength channel to ensure the accurate detection of Stokes vectors. A polymer true zero-order quarter-wave plate was employed to validate measurement accuracy and repeatability. Additionally, stress-induced birefringence in a PMMA arch-shaped workpiece was measured both before and after the application of force. Experimental results revealed that the repeatability of birefringence retardance and fast-axis azimuthal angle was better than 0.67 nm and 0.08°, respectively. This approach enables multispectral wavelength, high-speed, high-precision, and high-repeatability birefringence imaging measurements through a single imaging session. [ABSTRACT FROM AUTHOR]

With the increasing number of ongoing space missions for Earth Observation (EO), there is a need to enhance data products by combining observations from various remote sensing instruments. We introduce a new Transformer-based approach for data fusion, achieving up to a 10- to-30-fold increase in the spatial resolution of our hyperspectral data. We trained the network on a synthetic set of Sentinel-2 (S2) and Sentinel-3 (S3) images, simulated from the hyperspectral mission EnMAP (30 m resolution), leading to a fused product of 21 bands at a 30 m ground resolution. The performances were calculated by fusing original S2 (12 bands, 10, 20, and 60 m resolutions) and S3 (21 bands, 300 m resolution) images. To go beyond EnMap's ground resolution, the network was also trained using a generic set of non-EO images from the CAVE dataset. However, we found that training the network on contextually relevant data is crucial. The EO-trained network significantly outperformed the non-EO-trained one. Finally, we observed that the original network, trained at 30 m ground resolution, performed well when fed images at 10 m ground resolution, likely due to the flexibility of Transformer-based networks. [ABSTRACT FROM AUTHOR]

The document "Remote Sensing of Target Object Detection and Identification II" explores the importance of detecting and identifying target objects in remote sensing systems for applications such as environmental monitoring, urban monitoring, hazard management, and defense. It discusses the use of satellite, airborne, and unmanned aerial vehicle (UAV) platforms for data acquisition, with UAV platforms growing in popularity due to their cost-effectiveness. The research in this field utilizes different imaging technologies, including synthetic aperture radar (SAR), multispectral and hyperspectral imaging, and visible and near-infrared (VNIR) imaging. The document also highlights the use of deep learning algorithms, particularly convolutional neural networks (CNNs) and Transformers, for object detection and tracking tasks. Various articles in the document focus on ship detection in remote sensing images, small target detection in infrared images, and target tracking in image sequences. The methods and studies presented in the document are summarized, discussing their innovations, limitations, and potential future developments. Overall, the document provides a summary of different contributions related to remote sensing and image processing techniques, addressing specific challenges and presenting novel methods to enhance detection and tracking accuracy in various scenarios. [Extracted from the article]

Mangrove ecosystems play an irreplaceable role in coastal environments by providing essential ecosystem services. Diverse mangrove species have different functions due to their morphological and physiological characteristics. A precise spatial distribution map of mangrove species is therefore crucial for biodiversity maintenance and environmental conservation of coastal ecosystems. Traditional satellite data are limited in fine-scale mangrove species classification due to low spatial resolution and less spectral information. This study employed unmanned aerial vehicle (UAV) technology to acquire high-resolution multispectral and hyperspectral mangrove forest imagery in Guangxi, China. We leveraged advanced algorithms, including RFE-RF for feature selection and machine learning models (Adaptive Boosting (AdaBoost), eXtreme Gradient Boosting (XGBoost), Random Forest (RF), and Light Gradient Boosting Machine (LightGBM)), to achieve mangrove species mapping with high classification accuracy. The study assessed the classification performance of these four machine learning models for two types of image data (UAV multispectral and hyperspectral imagery), respectively. The results demonstrated that hyperspectral imagery had superiority over multispectral data by offering enhanced noise reduction and classification performance. Hyperspectral imagery produced mangrove species classification with overall accuracy (OA) higher than 91% across the four machine learning models. LightGBM achieved the highest OA of 97.15% and kappa coefficient (Kappa) of 0.97 based on hyperspectral imagery. Dimensionality reduction and feature extraction techniques were effectively applied to the UAV data, with vegetation indices proving to be particularly valuable for species classification. The present research underscored the effectiveness of UAV hyperspectral images using machine learning models for fine-scale mangrove species classification. This approach has the potential to significantly improve ecological management and conservation strategies, providing a robust framework for monitoring and safeguarding these essential coastal habitats. [ABSTRACT FROM AUTHOR]

In Central Europe, the extent of bark beetle infestation in spruce stands due to prolonged high temperatures and drought has created large areas of dead trees, which are difficult to monitor by ground surveys. Remote sensing is the only possibility for the assessment of the extent of the dead tree areas. Several options exist for mapping individual dead trees, including different sources and different processing techniques. Satellite images, aerial images, and images from UAVs can be used as sources. Machine and deep learning techniques are included in the processing techniques, although models are often presented without proper realistic validation.This paper compares methods of monitoring dead tree areas using three data sources: multispectral aerial imagery, multispectral PlanetScope satellite imagery, and multispectral Sentinel-2 imagery, as well as two processing methods. The classification methods used are Random Forest (RF) and neural network (NN) in two modalities: pixel- and object-based. In total, 12 combinations are presented. The results were evaluated using two types of reference data: accuracy of model on validation data and accuracy on vector-format semi-automatic classification polygons created by a human evaluator, referred to as real Ground Truth. The aerial imagery was found to have the highest model accuracy, with the CNN model achieving up to 98% with object classification. A higher classification accuracy for satellite imagery was achieved by combining pixel classification and the RF model (87% accuracy for Sentinel-2). For PlanetScope Imagery, the best result was 89%, using a combination of CNN and object-based classifications. A comparison with the Ground Truth showed a decrease in the classification accuracy of the aerial imagery to 89% and the classification accuracy of the satellite imagery to around 70%. In conclusion, aerial imagery is the most effective tool for monitoring bark beetle calamity in terms of precision and accuracy, but satellite imagery has the advantage of fast availability and shorter data processing time, together with larger coverage areas. [ABSTRACT FROM AUTHOR]

In recent years, deep learning techniques for pansharpening multiresolution images have gained increasing interest. Due to the lack of ground truth data, most deep learning solutions rely on synthetic reduced-resolution data for supervised training. This approach has limitations due to the statistical mismatch between real full-resolution and synthetic reduced-resolution data, which affects the models' generalization capacity. Consequently, there has been a shift towards unsupervised learning frameworks for pansharpening deep learning-based techniques. Unsupervised schemes require defining sophisticated loss functions with at least two components: one for spectral quality, ensuring consistency between the pansharpened image and the input multispectral component, and another for spatial quality, ensuring consistency between the output and the panchromatic input. Despite promising results, there has been limited investigation into the interaction and balance of these loss terms to ensure stability and accuracy. This work explores how unsupervised spatial and spectral consistency losses can be reliably combined preserving the outocome quality. By examining these interactions, we propose a general rule for balancing the two loss components to enhance the stability and performance of unsupervised pansharpening models. Experiments on three state-of-the-art algorithms using WorldView-3 images demonstrate that methods trained with the proposed framework achieve good performance in terms of visual quality and numerical indexes. [ABSTRACT FROM AUTHOR]

Pansharpening refers to the process of fusing multispectral (MS) images with panchromatic (PAN) images to obtain high-resolution multispectral (HRMS) images. However, due to the low correlation and similarity between MS and PAN images, as well as inaccuracies in spatial information injection, HRMS images often suffer from significant spectral and spatial distortions. To address these issues, a pansharpening method based on multimodal texture correction and adaptive edge detail fusion is proposed in this paper. To obtain a texture-corrected ( T C ) image that is highly correlated and similar to the MS image, the target-adaptive CNN-based pansharpening (A-PNN) method is introduced. By constructing a multimodal texture correction model, intensity, gradient, and A-PNN-based deep plug-and-play correction constraints are established between the T C and source images. Additionally, an adaptive degradation filter algorithm is proposed to ensure the accuracy of these constraints. Since the T C image obtained can effectively replace the PAN image and considering that the MS image contains valuable spatial information, an adaptive edge detail fusion algorithm is also proposed. This algorithm adaptively extracts detailed information from the T C and MS images to apply edge protection. Given the limited spatial information in the MS image, its spatial information is proportionally enhanced before the adaptive fusion. The fused spatial information is then injected into the upsampled multispectral (UPMS) image to produce the final HRMS image. Extensive experimental results demonstrated that compared with other methods, the proposed algorithm achieved superior results in terms of both subjective visual effects and objective evaluation metrics. [ABSTRACT FROM AUTHOR]

Pansharpening, a pivotal task in remote sensing, involves integrating low-resolution multispectral images with high-resolution panchromatic images to synthesize an image that is both high-resolution and retains multispectral information. These pansharpened images enhance precision in land cover classification, change detection, and environmental monitoring within remote sensing data analysis. While deep learning techniques have shown significant success in pansharpening, existing methods often face limitations in their evaluation, focusing on restricted satellite data sources, single scene types, and low-resolution images. This paper addresses this gap by introducing PanBench, a high-resolution multi-scene dataset containing all mainstream satellites and comprising 5898 pairs of samples. Each pair includes a four-channel (RGB + near-infrared) multispectral image of 256 × 256 pixels and a mono-channel panchromatic image of 1024 × 1024 pixels. To avoid irreversible loss of spectral information and achieve a high-fidelity synthesis, we propose a Cascaded Multiscale Fusion Network (CMFNet) for pansharpening. Multispectral images are progressively upsampled while panchromatic images are downsampled. Corresponding multispectral features and panchromatic features at the same scale are then fused in a cascaded manner to obtain more robust features. Extensive experiments validate the effectiveness of CMFNet. [ABSTRACT FROM AUTHOR]

The new generation of remote sensing satellites improves the coverage of remote sensing imaging by improving the orbital surface and using the rotary scanning imaging technology. In this paper, a new technical scheme of rotating payload platform based on disturbance-free payload technology is proposed to adapt to the rotary scanning imaging satellite. The platform adopts the cubic Stewart configuration and uses the Lorentz force magnetic actuator as the actuator to form the satellite support module and the payload into a noncontact two-stage three-body. First, this paper uses Newton-Euler method to establish a six-degree-of-freedom dynamic model for the new rotating payload platform, and establishes a kinematic model by quaternion method. Then, according to the established kinematics and dynamics model, the control scheme of absolute pointing control of payload platform (upper platform) and relative follow-up control of kinetic energy container (middle platform) is designed. Furthermore, the six-degree-of-freedom decoupling control method of the actuator is designed. Based on this, the absolute pointing controller of the payload module and the relative position and attitude calculate controller of the kinetic energy container are designed. Finally, the research of simulation is carried out. The simulation results show that the new rotating payload platform has higher rotation pointing accuracy and stability than the traditional ultra-quiet platform, and the vibration isolation acting on the support module and the vibration absorption acting on the payload are more effective. [ABSTRACT FROM AUTHOR]

This paper describes a prototype network of automated in situ measurements of hyperspectral water reflectance suitable for satellite validation and water quality monitoring. Radiometric validation of satellite-derived water reflectance is essential to ensure that only reliable data, e.g., for estimating water quality parameters such as chlorophyll a concentration, reach end-users. Analysis of the differences between satellite and in situ water reflectance measurements, particularly unmasked outliers, can provide recommendations on where satellite data processing algorithms need to be improved. In a massively multi-mission context, including Newspace constellations, hyperspectral missions and missions with broad spectral bands not designed for "water colour", the advantage of hyperspectral over multispectral in situ measurements is clear. Two hyperspectral measurement systems, PANTHYR (based on the mature TRIOS/ RAMSES radiometer) and HYPSTAR® (a newly designed radiometer), have been integrated here in theWATERHYPERNET network with SI-traceable calibration and characterisation. The systems have common data acquisition protocol, data processing and quality control. The choice of validation site and viewing geometry and installation considerations are described in detail. Three demonstration cases are described: 1. PANTHYR data from two sites are used to validate Sentinel-2/MSI (A- 2. HYPSTAR® data at six sites are used to validate Sentinel-3/OLCI (A- 3. PANTHYR and HYPSTAR® data in Belgian North Sea waters are used to monitor phytoplankton parameters, including Phaeocystis globosa, over two 5 month periods. Conclusion are drawn regarding the quality of Sentinel-2/MSI and Sentinel-3/OLCI data, including indications where improvements could be made. For example, a positive bias (mean difference) is found for ACOLITE_DSF processing of Sentinel-2 in clear waters (Acqua Alta) and clues are provided on how to improve this processing. The utility of these in situ measurements, even without accompanying hyperspectral satellite data, is demonstrated for phytoplankton monitoring. The future evolution of the WATERHYPERNET network is outlined, including geographical expansion, improvements to hardware reliability and to the measurement method (including uncertainty estimation) and plans for daily distribution of near real-time data. [ABSTRACT FROM AUTHOR]

Chlorophyll monitoring is an important topic in phenotypic research. For fruit trees, chlorophyll content can reflect the real-time photosynthetic capacity, which is a great reference for nutrient status assessment. Traditional in situ estimation methods are labor- and time-consuming. Remote sensing spectral imagery has been widely applied in agricultural research. This study aims to explore a transferable model to estimate canopy SPAD across growth stages and tree species. Unmanned aerial vehicle (UAV) system was applied for multispectral images acquisition. The results showed that the univariate model yielded with Green Normalized Difference Vegetation Index (GNDVI) gave valuable prediction results, providing a simple and effective method for chlorophyll monitoring for single species. Reflection features (RF) and texture features (TF) were extracted for multivariate modeling. Gaussian Process Regression (GPR) models yielded better performance for mixed species research than other algorithm models, and the R² of the RF+TF+GPR model was approximately 0.7 in both single and mixed species. In addition, this method can also be used to predict canopy SPAD over various growth stages, especially in the third and fourth stages with R² higher than 0.6. This paper highlights the importance of using RF+TF for canopy feature expression and deep connection exploration between canopy features with GPR algorithm. This research provides a universal model for canopy SPAD inversion which can promote the growth status monitoring and management of fruit trees. [ABSTRACT FROM AUTHOR]

Hyperspectral images can be widely used in many fields due to their high information richness. However, costly and complex imaging spectrometers limit its growth. Hyperspectral reconstruction aims to obtain the corresponding hyperspectral image from the multispectral image, to reduce the acquisition cost of hyperspectral images. At present, the related works mainly use methods based on deep learning, and they have achieved good results. However, how to fully extract the global spectral and spatial features of hyperspectral images is still not well solved. To address these issues, we propose an Attention and Transformer Complementary Fusion Network (ATCFNet), which is composed of three modules: Multi-angle Input Image Processing (MIIP), Deep Feature Extraction (DFE) and Hyperspectral Reconstruction (HR) modules. Within the DFE module, an improved Transformer module and a novel Multi-scale Spatial Attention (MSA) module are proposed to extract the global spectral relationship and the spatial features of the hyperspectral images, respectively. Moreover, the MIIP module is proposed to extract the features of input multispectral images more effectively and comprehensively. To verify these, we compare the proposed ATCFNet with other excellent reconstruction methods on three hyperspectral image datasets. The results show that our method achieves the best results in these datasets. Code is publicly available at . [ABSTRACT FROM AUTHOR]

Visual object tracking, pivotal for applications like earth observation and environmental monitoring, encounters challenges under adverse conditions such as low light and complex backgrounds. Traditional tracking technologies often falter, especially when tracking dynamic objects like aircraft amidst rapid movements and environmental disturbances. This study introduces an innovative adaptive multimodal image object-tracking model that harnesses the capabilities of multispectral image sensors, combining infrared and visible light imagery to significantly enhance tracking accuracy and robustness. By employing the advanced vision transformer architecture and integrating token spatial filtering (TSF) and crossmodal compensation (CMC), our model dynamically adjusts to diverse tracking scenarios. Comprehensive experiments conducted on a private dataset and various public datasets demonstrate the model's superior performance under extreme conditions, affirming its adaptability to rapid environmental changes and sensor limitations. This research not only advances visual tracking technology but also offers extensive insights into multisource image fusion and adaptive tracking strategies, establishing a robust foundation for future enhancements in sensor-based tracking systems. [ABSTRACT FROM AUTHOR]