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The birefringence in the cubic phase of BaTiO 3 has been measured as a function of temperature. The results have been interpreted in terms of the anisotropy of polarization fluctuation that appears as a precursor of the phase transition.

We generated transgenic mice expressing chimeric receptors, which comprise extracellular domains of the human granulocyte–macrophage colony-stimulating factor (hGM-CSF) receptor and transmembrane and cytoplasmic domains of the mouse leukemia inhibitory factor receptor. In suspension cultures of lineage-negative (Lin−), 5-fluorouracil-resistant bone marrow cells of the transgenic mice, a combination of hGM-CSF and stem cell factor (SCF) induced exponential expansions of mixed colony-forming unit. The combination of hGM-CSF and SCF was effective on enriched, Lin−Sca-1+c-kit+progenitors and increased either mixed colony-forming unit or cobblestone area–forming cells. In case of stimulation with hGM-CSF and SCF, interleukin-6 (IL-6) and SCF, or IL-11 and SCF, the most efficient expansion was achieved with hGM-CSF and SCF. When Lin−Sca-1+c-kit+CD34−further enriched progenitors were clone sorted and individually incubated in the presence of SCF, hGM-CSF stimulated a larger number of cells than did IL-6, IL-6 and soluble IL-6 receptor (IL-6R), or IL-11. These data suggest the presence of IL-6Rα-, IL-11Rα-, and gp130-low to -negative primitive hematopoietic progenitors. Such primitive progenitors are equipped with signal transduction molecules and can expand when these chimeric receptors are genetically introduced into the cells and stimulated with hGM-CSF in the presence of SCF.

The electronic structures of hydrogen storage alloys are calculated by the DV-Xα molecular orbital method. The results revealed that hydrogen interacts more strongly with hydride-non-forming elements, B, (e.g. Ni, Mn, Fe) than hydride-forming elements, A, (e.g. La, Zr, Ti, Mg) in every hydrogen storage alloy, despite there being a larger affinity of A elements for hydrogen than B elements in the binary metal-hydrogen system. The presence of such a metal-hydrogen interaction is a characteristic of hydrogen storage alloys. Furthermore, the A/B compositional ratio of hydrogen storage alloys can be understood in terms of a simple parameter, 2Bo(A-B)/[Bo(A-A)+Bo(B-B)], where the Bo(A-B), Bo(A-A) and Bo(B-B) are the bond orders between atoms given in the parentheses.

The safety and immunogenicity of Japanese encephalitis (JE) vaccine (Nakayama strain, monovalent / BIKEN) was studied in 538 U.S. soldiers in 1990. Three doses of vaccine from three consecutively manufactured lots were given on days 0, 7, and either 14 or 30. Serum for antibody determination was drawn at months 0, 2, and 6. Japanese encephalitis plaque reduction neutralization tests were performed by three laboratories on each specimen. Five hundred twenty-eight (98%) participants completed the immunization series. All recipients without antibody before immunization developed neutralizing antibody against JE virus. There were no differences in geometric mean titer among the three test lots at months 2 and 6. Soldiers who received the third dose on day 30 had higher titers at both time points. Antibody to yellow fever had no significant effect on immune response to vaccine. Conclusions drawn from analysis of serologic data from the three labs were nearly identical. Symptoms were generally limited to mild local effects and were reduced in frequency with each subsequent does in the series (21% to 11%; P < 0.0001). Generalized symptoms were rare (e.g., fever = 5%) with no reported cases of anaphylaxis.

The present experiment was conducted to clarify the effect of bovine oviductal epithelial cells (BOEC) collected from oviducts ipsilateral to cystic follicles (CFs) using an in vitro coculture system on the development of in vitro matured/fertilized (IVM/IVF) bovine embryos. In the first comparison, the effect of the presence of CF on the development of the embryos cocultured with BOEC derived from the cows with CF (n = 18) and corpus hemorrhagicum (CH, n = 10) was examined. In the second comparison, the effect of the type of cyst [progesterone (P4)-dominant; n = 9, estradiol-17beta (E2)-dominant; n = 5] on the development of the embryos cocultured with BOEC derived from the cystic cows was examined. No difference was observed between CF and CH (control) groups in the mean developmental rates of embryos developed to > or =2-cell (86.3% vs. 86.4%), 8-16 cells (53.0% vs. 56.2%), blastocyst (24.2% vs. 24.8%) and hatched blastocyst (12.0% vs. 14.6%). However, the blastocyst production rate was significantly different (P

In the Current Sustaining Tokamak of the Nagoya University (CSTN)-IV research tokamak system, using a compact 40-kHz pulse width modulation (PWM) inverter power supply, which is controlled through the LabVIEW program, we construct a new tokamak discharge system with multimode including a stable alternating current discharge and a high-repetition high-duty one. These discharge modes can be operated continuously for as long as 60 seconds. The continuous discharge of long duration is able to simulate the important physical and chemical processes of long discharges in fusion devices, in which the heat load to the wall and the particle balance in the plasma-wall system are crucial topics in order to realize a long pulse fusion reactor, like ITER. An ergodic divertor is one of the tools used to control the particle balance and the heat load to the wall. In addition, we installed another inverter power supply to generate a rotating magnetic perturbation for a dynamic ergodic divertor (DED) with the appropriate measurement system so that we could carry out experiments on heat and particle control with DED in prolonged operation. © 2000 Scripta Technica, Electr Eng Jpn, 133(4): 8–18, 2000

Objective To investigate the specificity and sensitivity of the combination of redistribution in exercise thallium-201 single photon emission computed tomography (SPECT) and exercise induced ST elevation for detecting the viable myocardium in patients with acute myocardial infarction. Design 37 patients were studied within seven weeks of onset of Q wave myocardial infarction (anterior in 22, inferior in 15). All patients underwent exercise four hour redistribution thallium-201 SPECT and positron emission tomography using fluorine-18-fluorodeoxyglucose (FDG) and nitrogen-13 ammonia under fasting conditions. Results Sixteen patients showed exercise induced ST elevation ⩾ 1.5 mm, and 15 of these had increased FDG uptake in the infarct region. Eleven of 16 patients (10 of 11 patients with anterior infarctions) with irreversible thallium-201 defects and increased FDG uptake showed exercise induced ST elevation. The sensitivity, specificity, and predictive accuracy of redistribution, exercise induced ST segment elevation, or both for detecting increased FDG uptake were 82%, 75%, and 67% (94%, 75%, and 91% for anterior infarctions), respectively. Conclusions In patients with acute Q wave myocardial infarction, the combination of redistribution in exercise thallium-201 SPECT and exercise induced ST elevation can detect the viable myocardium in the infarct region with high sensitivity and specificity, especially in patients with anterior infarctions.

The influence of metatarsophalangeal lesions on the position of the center of plantar pressure in rheumatoid patients was analyzed by a photoelastic method. Fifteen patients were divided radiographically into three groups (A to C) based on the degree of destruction of the metatarsophalangeal joints. Fifteen age-matched non-rheumatoid subjects were studied as a control group. As parameters expressing the position of the centre of plantar pressure, longitudinal ratio (λ) and transverse ratio (η) were defined and analyzed respectively. the λ of group B was significantly lower than those of group A and C (A: 44.6 ± 6.4, B: 39.0 ± 0.5, P = 0.0327; B: 39.0 ± 0.5, C: 44.7 ± 4.9, P = 0.0075). The η of group B was significantly lower than those of group A and C (A: 46.2 ± 3.4, B: 43.4 ± 7.4, P = 0.0161; B: 43.4 ± 7.4, C: 46.4 ± 1.9, P = 0.0075). By comparing the above data with the control data and combinations of patterns in both parameters, the positions of the centre of plantar pressure were divided into four types. The most common type in group A and C was ‘anterolateral (steady)', and that in group B was ‘posteromedial (unsteady)'. Based on the result, we conclude that the treatment of the lesions in group B is the most necessary to maintain the stability of standing.

The viability of embryos before flushing from donor mares (n = 5) and after transfer to recipient mares (n = 7) was monitored in mare serum by detecting early pregnancy factor (EPF) using the rosette inhibition test (RIT). The EPF activity was measured in donor mares before and after natural mating at natural estrus; after ovulation on Days 2, 5 and 8; and after embryo flushing (Day 8) on Days 8, 9, 10 and 13 after ovulation. The collected embryos were transferred immediately after flushing. The EPF activity in recipient mares were measured on the day of transfer and after embryo transfer on Days 1, 2, 3 and 5. Pregnancy was confirmed on Day 12 to 14 after embryo transfer. The mean EPF activity of donor mares was increased to the pregnant level (> an RI titer score of 10) on Day 2 after ovulation. Two days after flushing the embryos, the EPF activity of donor mares had decreased to the nonpregnant level. Among the 7 recipient mares, 3 mares were diagnosed pregnant on Day 12 after embryo transfer with ultrasound. The EPF activity of the pregnant recipient mares was increased above the minimum level observed in pregnant mares on Days 2 to 3 after transfer. However, among the nonpregnant recipient mares after embryo transfer, the EPF activity of 3 mares remained at the pregnant level only 2 to 3 d and then declined to the nonpregnant level. In one recipient mare, EPF activity did not reach the pregnant level throughout the sample collection. The results of this study indicated that equine EPF can be detected in serum of pregnant mares as early as Day 2 after ovulation. From our observation, we conclude that the measurement of EPF activity is useful for monitoring the in vivo viability of equine embryos and early detection of embryonic death.

Objective—To investigate the relations between myocardial metabolism and the kinetics of thallium-201 in myocardial scintigraphy. Methods—46 patients within six weeks after the onset of acute myocardial infarction underwent resting myocardial dual isotope, single acquisition, single photon emission computed tomography (SPECT) using radioiodinated 15-iodophenyl 3-methyl pentadecaenoic acid (BMIPP) and thallium-201, exercise thallium-201 SPECT, and positron emission tomography (PET) using nitrogen-13 ammonia (NH3) and [F18]fluorodeoxyglucose (FDG) under fasting conditions. The left ventricle was divided into nine segments, and the severity of defects was assessed visually. Results—In the resting SPECT, less BMIPP uptake than thallium-201 uptake was observed in all of 40 segments with reverse redistribution of thallium-201, and in 21 of 88 segments with a fixed defect of thallium-201 (p

The present study was conducted with ovaries to evaluate the effect of the presence of a cystic follicle and its steroid hormone concentration on the oocyte recovery rates, oocyte morphology and in vitro maturation of the oocytes from coexisting small follicles. Ovaries, each bearing a follicular cyst (containing > 10 ml of follicular fluids, > 3 cm in diameter, and without a functional corpus luteum), were collected from each of the 26 Holstein cows from a local slaughterhouse. Small follicles (1-7 mm) from these ovaries were aspirated and their cumulus-oocyte complexes (COCs) classified into one of 5 groups (A to E), depending on oocyte and cumulus cell morphologies. Those oocytes with compact cumulus cells were cultured and their maturation rates determined. Concentrations of estradiol-17 beta (E2) and progesterone (P4) were measured in cystic follicular fluid using double antibody enzyme immunoassays (EIA). The morphology of the COCs and maturation rates of the oocytes were then evaluated using two comparisons. In first comparison, the left and right ovaries obtained from an individual cow were considered as a pair, with each pair being divided into two groups depending on the presence or absence of an E2 dominant or P4 dominant cystic follicle in one or another of the ovaries: E2 dominant cows; and P4 dominant cows. Oocytes collected from the ovaries of cyclic cows at follicular, luteal and post-ovulatory stages served as controls. The oocyte recovery rates, oocyte morphologies and oocyte maturation rates were independent of the presence or absence of a coexisting cystic follicle, or its steroid hormone classification or concentration. In the second comparison, each ovary was consider individually and divided into one of the two classes, depending on the presence or absence of a cystic follicle. Those ovaries with cystic follicles were then divided into three subclasses on the basis of E2 and P4 concentrations in the cystic follicular fluids: P4 dominant (P4/E2 ratio > 1); E2 dominant (P4/E2 ratio < 1); and both P4 and E2 dominant follicles present. The numbers of oocytes obtained from an ovary, their morphology and their maturation rates were not significantly different in the presence or absence of a coexisting cystic follicle. Moreover, the number of the oocytes aspirated from an ovary, their morphology and their maturation rates in small follicles coexisting with P4 dominant, E2 dominant and both P4 and E2 dominant cystic follicles were also not different. These results suggested that neither the presence of a cystic follicle in a cow's ovary nor the cyst's steroid hormone concentrations affected the oocyte recovery rate, oocyte morphology and maturation rates of the coexisting small follicles.

This study was conducted to assess the ability of the follicular fluid derived from large and small follicles to support the in vitro oocyte maturation and subsequent fertilization and developmental capacity. Oocytes were cultured in bovine follicular fluid aspirated from small (SFF; 2-5 mm in diameter), large (LFF; 10 to 20 mm in diameter) follicles and TCM199 as a control under 5% CO2 in air. All maturation media were supplemented with 1 IU ml-1 pregnant mare serum gonadotropin. After 24 h culture, oocytes were fertilized in vitro with frozen-thawed and heparin-treated (10 micrograms ml-1, 15 min) bull spermatozoa and cultured in TCM199 with bovine oviductal epithelial cells (BOEC) for 7 days. Maturation of bovine oocytes cultured in LFF was inhibited and the low of male pronucleus formation was observed when compared with that of SFF (maturation rate: 69 vs. 78%; P0.05; male pronucleus formation rate: 58 vs. 80%; P0.05). Developmental capacity of bovine oocytes cultured in SFF was significantly (P0.05) higher than that of LFF (15 vs. 5%), but significantly (P0.05) lower than that of the control. There were no differences in the number of nuclei per blastocyst obtained after each treatment. These results indicate that the inhibitory action of follicular fluid on in vitro maturation, male pronucleus formation and developmental capacity of bovine oocytes is dependent on the developmental stage of the follicles from which fluid was obtained.

We propose a model for a magnetic recording channel to estimate the influences of the nonlinear distortion of an MR head on the performance of the PRML system, using a superposition of a magnetic field and a transfer curve obtained by a micro-magnetic model. A readback signal was simulated using a nonlinear model. The simulated signal was compared with an actual readback signal in the analysis of the isolated pulse, frequency response, and extracted dipulse response. We found that the nonlinear model represents actual MR head output satisfactorily, and that the performance of the PR4ML system was affected by the nonlinear distortion of the MR head for a 5% loss of the amplitude margin when the asymmetry was within 10%.

Hydroxyapatite sol was prepared by dispersing unheated hydroxyapatite microcrystals into distilled water. A suspension of hydroxyapatite heated at 800 degrees C (HAp-800 degrees C) in distilled water was used as a comparative material. These were characterized by X-ray diffractometry, SEM and electroconductivity measurements. 0.001 ml of each material was injected into the upper first molar pulp of rats. After 7-14 days post-operatively, the group with hydroxyapatite sol showed slight inflammation in the pulp cavities and a highly calcified tubular dentine bridge was observed. Osteodentine had developed and adhered to the surface of the hydroxyapatite microcrystals. Matrix vesicles were observed in the extracellular matrix between collagen fibrils and electron-dense granules. After 28 days, the inflammation completely disappeared and tubular dentine was observed below the osteodentine. Acid phosphates (ACPase) activity was detected along all biosynthesizing pathways in odontoblast-like cells. Localized dehydrogenate (LDH) activity indicated phagocytosis of hydroxyapatite microcrystals by odontoblasts. In the group with HAp-800 degrees C after 7-14 days post-operatively, the inflammation was moderate. After 28 days, relatively fewer calcified dentine tubules were observed. ACPase activity was detected only along the membranes bounding cytoplasmatic vacuoles. It was concluded that hydroxyapatite sol is a safe biomaterial for use as dental pulp and induces early formation of dentine-bridge more so than HAp-800 degrees C.

TiO2SiO2MgO catalyst was prepared and the OH radicals formed on the catalyst contacted with water were studied by the spin-trapping method. The condition of the catalyst surface that can produce OH radicals is optimized. The initial concentration of OH radicals on the surface does not influence the rate of the photocatalytic decomposition of hydrogen peroxide.

The simulated gas target divertor experiment has been performed to investigate the fundamental physics of plasma detachment in the linear plasma device, TPD-I, which has a high heat flux and high density plasma in steady state. The existence of a density threshold for plasma detachment was observed in our experiment. It is found that the electron-ion temperature relaxation process is a key to determine the density dependence of the plasma detachment.

The progress of laser fusion research at the Institute of Laser Engineering, Osaka University is reviewed. They are physics investigations on target implosion, development of laser technologies, and R&D for laser fusion power plants. The long term strategy of Inertial Fusion Energy (IFE) development has been reexamined taking into account the recent progress of implosion physics and reactor related technologies. 15 refs., 12 figs.

Changes and local distribution of oviductal progesterone (P(4)) concentration during the estrous cycle and early pregnancy in cows were investigated. Intact reproductive tracts were collected from 16 Holstein cows at an abattoir. Samples were classified in to 4 stages (follicular, postovulatory, luteal and early pregnant,< 20 d) based on visual observation of corpus luteum (CL), uterine characteristics and luteal P(4) concentrations. Oviducts were separated from the uterus at the utero-tubal junction and divided into 4 parts: fimbriae, proximal, medial and distal parts. Luteal tissue samples were also collected. Progesterone levels in oviductal and luteal tissues were determined by radioimmunoassay (RIA). Comparatively higher (P < 0.001) P(4) levels were found in stages with a functioning CL ( luteal phase and early pregnancy) than in those with a regressing CL (follicular phase and post ovulation). The oviduct ipsilateral to the CL bearing ovary during the luteal phase and early pregnancy showed higher ( P < 0.001) P(4) concentrations than the contralateral side. Such a difference was not observed during the follicular phase or post ovulation. The ipsilateral oviduct to the functioning CL at early pregnancy showed higher (P

McCune-Albright syndrome (MAS) is characterized by café-au-lait spot, multiple endocrine hyperfunction, and polyostotic fibrous dysplasia. A somatic point mutation of Gsalpha protein was reported to decrease GTPase activity, leading to increase in the GSalpha-associated hormone actions via cAMP. IL-6 is known to stimulate osteoclast formation and in the IL-6 promoter, a cAMP responsive element has been identified. In this paper, we investigated the role of IL-6 in the bone lesions of MAS, using the isolated fibrous cells from the polyostotic fibrous dysplasia tissues in bones of the two patients with MAS. Bone biopsy specimen revealed the increased osteoclast in number. In both patients, a GSalpha mutation (Arg201 -> His) was identified in the cultured fibrous cells. Intracellular cAMP content and IL-6 secretion by the patient cells were increased. Rp-8Br-cAMP significantly inhibited IL-6 production in the patient cells, while it had no effect on normal control. The addition of dibutyryl cAMP significantly increased the synthesis of IL-6 in normal control cells. In contrast, no effect of dibutyryl cAMP on IL-6 synthesis was observed in the cells from one of the MAS patients. These data suggest that IL-6 is, at least, one of the downstream effectors of cAMP and that the increased IL-6 synthesis has a pathogenic role in the bone lesions of MAS patients via increasing the number of osteoclasts. These results may provide a new strategy for the therapy of MAS patients.

Purpose: The value of coronary angiography in the diagnosis of thrombus in the left atrial appendage (LAA) was retrospectively analyzed. Material and Methods: The study covers 34 patients in whom coronary angiography showed coronary neovascularity in LAA with coronary artery-left atrial fistula indicating LAA thrombus. All 34 patients underwent transthoracic echocardiography within one week of coronary angiography. Open-heart surgery was undertaken 2–31 months after angiography in 28 patients. Results: Coronary neovascularity and coronary artery-left atrial fistula arose from the left circumflex artery in 28 patients, and from the left circumflex artery and the right coronary artery in the remaining 6 patients. By echocardiography, LAA thrombus was detected in only one of the 34 patients. In 18 of the 28 patients who underwent open-heart surgery, LAA thrombus was found at surgery to have resolved. Conclusion: Coronary angiography is useful in the diagnosis of LAA thrombus, and coronary neovascularity and fistula formation may indicate that the thrombus can spontaneously resolve.

The reverse transcriptase polymerase chain reaction (RT-PCR) was applied to detect contamination of Marek's disease (MD) vaccine with reticuloendotheliosis virus (REV). The env primers were used for the 1st RT-PCR to amplify the DNA fragments of REV-A and -T. The rel and env primers were used for nested-PCR to confirm the sites deleted from REV-T and REV-A. Specific amplification products were detected in the 1st RT-PCR with these primers. By nested PCR with the env and the rel primer pairs, the products originating from REV-A and -T were identified. This system, using the env primer pairs, showed a specific amplification with several REV strains (REV-T, DE, CE, KI and 0202), but no amplified product was detected with MDV, NDV, IBV or ILTV. The 1st RT-PCR detected the virus in a concentration of 10(3) in 50% fluorescent antibody infectious dose per ml (FAID50/ml) and the nested PCR detected 10(1) FAID50/ml virus. The sensitivity of the RT-PCR system was found to be higher than that of the FA assay. This system provides a rapid, sensitive and specific method for detection of contamination of MD vaccines with REV-RNA, and it may be applied for quality control of live vaccines.

The Rhinogobius species complex (Pisces: Gobiidae) includes great variation in colour, morphology and ecological form. In particular, R. sp. OR (Orange type) exhibits some of the greatest colour variation among the species complex, although the genetic relationships among the variations in this fish remain unclear. Thirteen microsatellite loci were identified from R. sp. OR, and all loci were polymorphic with two to 17 alleles per locus. Observed heterozygosity ranged from 0.1 to 0.9, while the expected heterozygosity ranged from 0.19 to 0.96. Multiplex polymerase chain reaction reaction (PCR) were optimized. All loci except Rhi-5 conform to Hardy–Weinberg equilibrium (P > 0.05).

In this paper, a built-in electrical test circuit is proposed to detect an open defect at an interconnect between a land in a printed circuit board and an IC. An inverter gate is used as an open sensor in the test circuit. An AC voltage signal is provided to a targeted interconnect and the sensor as a test input signal to detect the defect. The defect is detected by means of supply current of the sensor. We examine by Spice simulation whether open defects can be detected with the test circuit. The results reveal us that an open defect can be detected at a test speed of 1 MHz for each interconnect.

It has been shown that the protein serine/threonine phosphatase type 2A alpha catalytic subunit (PP2A alpha) has a mutation consisting of a glycine substitution for cysteine at 269 in the okadaic acid resistant variant of CHO cells, and that the mutant protein is resistant to okadaic acid (Shima, H. et al. Proc. Natl. Acad. Sci. USA 91, 9267-9271, 1994). In this study we analyzed okadaic acid responsive sequences in PP2A alpha by introducing a mutation at around codon 269 of the cDNA strand. The recombinant mutant PP2A alpha proteins Y265F, C266G, Y267G and C269Y were resistant to okadaic acid, with IC50s of 10, 24, 20 and 10 nM, respectively, as opposed to the recombinant wild PP2A alpha protein which had an IC50 of 0.24 nM. This observation suggests that not only cysteine at 269, but also other YCY amino acids, between 265-267, are necessary for the high sensitivity of PP2A to okadaic acid.

Gas target experiments are performed in the TPD-I device, which produces a high density helium plasma ∼ 6 × 1019 m−3 with an electron temperature around 10 eV. The maximum plasma heat flow is estimated to be about 20 MW/m2. A strong reduction of electron density and temperature was observed by feeding a secondary helium gas into the plasma and a stable detached plasma is formed in front of the target plate. The radial profile of the electron temperature is changed from peaked to hollow as the gas pressure is increasing. These experimental results are compared with the plasma simulations performed with the 2D plasma fluid B2 code. Good agreement with the simulated radial profiles of the plasma parameters is found.

We have been developing an alternative scheme of fast ignition of inertial confinement targets with converging shock. Experiments were carried out on GEKKO-XII laser facility at ILE, Osaka University. We employed Au cone targets filled with low- density RF foam (2 mg/cm3). The foam-filled cone targets were irradiated by three beams of the GEKKO-XII, with pulse duration of 1.3 ns, intensity of ~ 1014 W/cm2 in 2 :λ(ω0.527μm). Self-emission at the tip of cone was observed by one-dimensional streaked optical pyrometer (SOP) and two-dimensional images.

Water intake structure is an important element technology for small hydroelectric generation. Currently, intake structures with bar screens have been broadly introduced; however, these require constant maintenance to avoid the clogging of bars by dust or gravel. This study considers the optimal structure of bottom intakes by focusing on two criteria: efficient water intake and prevention of clogs by trapping trash. Grating was selected as the intake structure because it is convenient to construct, widely available, and cheaper than other materials. A flume experiment was conducted to examine the relation between the grating structure and the intake efficiency and trash-trapping rate. Results indicate a clear linear relation between the installation angle and water intake capacity. Furthermore, the trash-trapping rate is low for gratings that have high opening area ratios because their surface areas are small and friction resistance is low.

Today, approximately three years since its development, the non‐contact micro soldering method (local soldering method) continues to be highly regarded due to its superiority over other soldering methods (i.e., soldering irons, etc.) and is being used in a wide range of applications in the industrial electronics field. This technology has been incorporated into the production lines of numerous plants worldwide including the USA and Japan. This paper focuses on introducing the following: (1) the micro spot soldering method, the method most frequently utilised in production lines; (2) the continuous soldering method newly developed for use with surface‐mount components such as connectors of PCMCIA memory cards; and (3) the upward soldering method in which the light beam is irradiated upward, enabling the components to be soldered without flipping printed circuit boards (PCBs). The paper will also include practical examples, the optimal circuit board design, and an evaluation of soldering quality.

We propose novel neutron source using high-intensity laser based on the cluster fusion scheme. We developed DPSSL-pumped high-repetition-rate 20-TW laser system and solid nanoparticle target for neutron generation demonstration. In our neutron generation experiment, high-energy deuterons were generated from coulomb explosion of CD solid- nanoparticles and neutrons were generated by DD fusion reaction. Efficient and stable neutron generation was obtained by irradiating an intense femtosecond laser pulse of >2×1018 W/cm2. A yield of ~105 neutrons per shot was stably observed during 0.1-1 Hz continuous operation.

The interleukin 3 (IL-3), IL-5, and granulocyte/macrophage colony-stimulating factor receptors consist of a cytokine-specific alpha subunit and the common beta subunit. Whereas IL-3 stimulates various lineages of hematopoietic cells, including multipotential progenitors, IL-5 acts mainly as an eosinophil lineage-specific factor. To investigate whether the lineage specificity of IL-5 is due to restricted expression of the IL-5 receptor alpha subunit (IL-5R alpha), we generated transgenic mice that express the mouse IL-5R alpha constitutively by phosphoglycerate kinase promoter. The transgenic mouse expressed IL-5R alpha ubiquitously, and the bone marrow cells formed various types of colonies, including multi-lineage colonies, in response to IL-5. IL-5 also supported formation of both multi-lineage and blast cell colonies from dormant progenitors of the 5-fluorouracil-treated transgenic mice. The cells composing the blast cell colony gave rise to many colonies including multi-lineage colonies when they were replated in secondary culture containing either Il-5 or IL-3. There was no significant difference in replating efficiency or in types of secondary colonies between IL-5- and IL-3-stimulated cultures. Conversely, the cells from the IL-3-induced blast cell colonies of the transgenic mice proliferated in response to either IL-3 or IL-5. Thus, the development of the progenitors can be equally supported by either IL-5 or IL-3, suggesting that intracellular signals from the IL-3R can be replaced by those from IL-5. These results strongly suggest that the lineage specificity of IL-5 is mainly due to the restricted expression of IL-5R alpha.

Malaria vector occurrence in mountainous villages was monitored for 2 years in north Thailand. Both larval and adult densities of Anopheles miniumus peaked in May or June and in November or December. Stable conditions in streams during these periods possibly favored the breeding of A n. miniumus. Higher lalval density also was encountered in streams where ca. 3/4 of the water surface was sunlit, portions with rapid currents and slow currents alternate, and the water waS clear. An. maculatus sensu lato peaked in June or July, while An. dirus scarcely changed seasonally. The densities of the three malaria vectors were higher in more mountainous villages.

A compact fast core heating experiment is described. A 4-J 0.4-ns output of a laser-diode-pumped high-repetition laser HAMA is divided into four beams, two of which counterilluminate double-deuterated polystyrene foils separated by $100\text{ }\text{ }\ensuremath{\mu}\mathrm{m}$ for implosion. The remaining two beams, compressed to 110 fs for fast heating, illuminate the same paths. Hot electrons produced by the heating pulses heat the imploded core, emitting x-ray radiations $g20\text{ }\text{ }\mathrm{eV}$ and yielding some ${10}^{3}$ thermal neutrons.

The telescience testbed experiments were carried out to test and investigate the tele-manipulation techniques in the intracellular potential recording of amphibian eggs. Implementation of telescience testbed was set up in the two separated laboratories of the Tsukuba Space center of NASDA, which were connected by tele-communication links. Manipulators respective for a microelectrode and a sample stage of microscope were moved by computers, of which command signals were transmitted from a computer in a remote control room. The computer in the control room was operated by an investigator (PI) who controlled the movement of each manipulator remotely. A stereoscopic vision of the microscope image were prepared by using a head mounted display (HMD) and were indispensable to the intracellular single cell recording. The fertilization potential of amphibian eggs was successfully obtained through the remote operating system.

Described are two approaches to make cryogenic inertial fusion targets that have a uniform solid or liquid fuel layer inside a spherical hollow fuel capsule. One is to utilize low‐density, low‐atomic‐number plastic foam to sustain liquid fuel (foam method) and the other is to initiate a glow discharge in the void of a solid fuel layer to symmetrize the solid layer (plasma layering method). In the foam method, we have successfully made a foam shell with a gas barrier to prevent the fuel from evaporation by means of density‐matched emulsion method followed by an interfacial polycondensation method. Foam shells having diameters of 600–1550 μm and wall thicknesses of 10–50 μm were coated with a normal density plastic layer having thickness of 4–10 μm. These foam shells were provided for implosion experiments with the Gekko XII glass laser system. In the plasma layering process, we examined the feasibility of the method by performing experiments using krypton gas and liquid nitrogen. A nonuniform solid krypton layer was redistributed into a uniform one after a 30 min discharge induced by an external 2.45 GHz microwave field. Experimental results on the heat generation in shells indicated that this technique can redistribute 10 K solid fuel into a uniform layer using intermittent discharges.

Summary Twenty‐two strains of Erysipelothrix rhusiopathiae, 14 strains of Erysipelothrix tonsillarum, and four other strains representing two serovars were examined for virulence in 30‐ to 40‐day‐old specific pathogen‐free chickens. Some of the chickens that were given strains of serovars la, 2, 5, 6, 8, 9, 15 or 21 of E. rhusiopathiae showed signs of disease, lesions, bacteraemia or recovery of the bacterium. In contrast, there were no clinical signs, pathological lesions or bacterial isolations in chickens inoculated with the E. tonsillarum or other strains. The study thus confirmed that strains of some serovars of E. rhusiopathiae are pathogenic for chickens, but that E. tonsillarum strains are not pathogenic and should be omitted from potential causes of erysipelas in chickens.

Abdominal ultrasonographic screening for cancer was performed in 6001 Hiroshima and Nagasaki A-bomb exposed and comparison subjects, all members of the Adult Health Study of the Radiation Effects Research Foundation, formerly the Atomic Bomb Casualty Commission. This study yielded 20 cancers, including 7 hepatocellular, 3 gastric, 3 renal and 2 urinary bladder cancers; one cancer each of the ovary, pancreas, colon and ureter; and one cancer metastatic to the liver, whose primary was unknown. Seventeen of these 20 subjects manifested no symptoms or signs of their disease. A variety of additional tumors, unproven and probably benign, including uterine myomata, and other abnormalities were also detected. Abdominal ultrasonographic screening greatly assisted in the detection of cancers, most of which neither the patients nor their physicians were aware. In this screened fixed population sample the cancer detection rate was 0.33%, exceeding any such rates previously reported in the medical literature.

We previously constructed an accurate displacement sensor for detection of eyelid movement, using an amorphous magnetic core multivibrator. Improvements in eyelid motion sensors are necessary to achieve independence of the magnetization direction of the cores as well as a broadened visual field. We constructed a second displacement sensor for detection of eyelid movement, this time using amorphous wire magneto-inductive (MI) elements. The elements were formed from tension-annealed zero-magnetostriction amorphous wires. The MI effect and the symmetrical MI characteristics of the elements were used to form thin sensor heads without coils. Inductances change independently of the magnet magnetization directions.

An amorphous alloy layer prepared by a laser-quenching technique was employed in the construction of a magnetostrictive torque sensor. Fe 78 B 13 Si 9 amorphous alloy layers were produced on stainless steel substrates by laser-quenching. The laser-quenched layers consisted of a mainly amorphous phase, partially including regions crystallized under the influence of heat (heat-affected zones). VSM measurements showed that the amorphous alloy layers have an axis of easy magnetization in the laser scanning direction. Taking account of this magnetic anisotropy, we prepared a torque sensor by scanning a laser beam over the shaft surface at 45° to the shaft axis. The torque sensor thus prepared exhibited sufficient sensitivity and good linearity in detecting static torque.

A case of osteoid osteoma (OO) in the mandible of 26-year-old female is presented. Her main complaint was intermittent pain during a 3 year period. The panoramic radiograph of the mandible showed diffuse sclerosis including an ill-defined circular radiopacity in the molar region. Computed tomography (CT) revealed an osseous lesion of 10 mm diameter that was located in the cortical bone of the mandible. Bone scintigraphy showed a localized region of high uptake of 99mTc HMDP. The lesion was diagnosed as an OO by histopathological examination of the surgical specimen and CT findings. Since radiological appearance has very significant meaning in the differentiation of an OO from other bone forming tumors, CT is the method of choice when the main symptom is pain and the conventional radiography was not informative.

The present study was conducted to 1) identify the natural source of feed contamination by zearalenone (ZEN), which was suspected to have caused persistently increased urinary ZEN concentrations in one of our experimental cattle herds, and 2) evaluate the effects of intervention against this source of contamination. As an experimental model, a fattening Japanese Black cattle herd showing persistently increased urinary ZEN concentrations was identified. Urinary ZEN concentrations of cows fed with new rice straw (experimental group, n = 6) vs. cows that continued to feed on the old rice straw (control group, n = 4) were measured at the start (d 1) and at 2 wk (d 14) after the onset of feeding with straw. In addition, the ZEN concentration in feed and water samples was measured by using both the ELISA and HPLC methods. Furthermore, isolation and identification of fungi from rice straw and concentrate feed samples were performed. The urinary ZEN concentration [ZEN (pg/mL)/creatinine (mg/mL) = pg/mg of creatinine] of cows fed with new rice straw was significantly (P0.05) less (843 pg/mg of creatinine) than that of cows fed with old rice straw (15,951 pg/mg of creatinine). On both d 1 and 14, the ZEN concentrations of old rice straw were greater than those of new rice straw. In addition, fungal colonies were observed in the culture media that was obtained from the old rice straw suspected of ZEN contamination, but not in the culture media from new rice straw or other feed samples. In conclusion, our field trials clearly indicate that the rice straw fed to the cows was naturally contaminated with ZEN, and that the monitoring of urinary ZEN concentrations could prove to be a useful tool for detecting the exposure of cattle to ZEN contamination at the farm level.