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381 results on '"Lopez-Ribot, Jose"'

105. High-Throughput Nano-Biofilm Microarray for Antifungal Drug Discovery

Author

ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX, Srinivasan, Anand, Leung, Kai P, Lopez-Ribot, Jose L, Ramasubramanian, Anand K, ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX, Srinivasan, Anand, Leung, Kai P, Lopez-Ribot, Jose L, and Ramasubramanian, Anand K

Abstract

Micro- and nanoscale technologies have radically transformed biological research from genomics to tissue engineering, with the relative exception of microbial cell culture, which is still largely performed in microtiter plates and petri dishes. Here, we present nanoscale culture of the opportunistic fungal pathogen Candida albicans on a microarray platform. The microarray consists of 1,200 individual cultures of 30 nl of C. albicans biofilms ( nano-biofilms ) encapsulated in an inert alginate matrix. We demonstrate that these nano-biofilms are similar to conventional macroscopic biofilms in their morphological, architectural, growth, and phenotypic characteristics. We also demonstrate that the nano-biofilm microarray is a robust and efficient tool for accelerating the drug discovery process: (i) combinatorial screening against a collection of 28 antifungal compounds in the presence of immunosuppressant FK506 (tacrolimus) identified six drugs that showed synergistic antifungal activity, and (ii) screening against the NCI challenge set small-molecule library identified three heretofore-unknown hits. This cell-based microarray platform allows for miniaturization of microbial cell culture and is fully compatible with other high-throughput screening technologies., Published in mBio, v4 n4 article e00331-13 p1-11, July/August 2013.

Published
2013

132. Colaboradores

Author

Abarca Salat, María Lourdes, Aguirre Urizar, José Manuel, Álvarez Blanco, Alfonso, Álvarez Duarte, Eduardo, Arechavala Silva, Alicia, Arenas, Roberto, Arévalo Morales, María del Pilar, Bonifaz Trujillo, Alexandro, Cabañes Sáenz, Francisco Javier, Calderón Sandubete, Enrique José, Cano Lira, José Francisco, Cantón Lacasa, Emilia, Carrillo Muñoz, Alfonso Javier, Castaño Navarro, Irene, Colom Valiente, María Francisca, Crespo Erchiga, Vicente, Cuenca Estrella, Manuel, De Las Peñas Nava, Alejandro, Díaz Jarabrán, María Cristina, Dolande Franco, Maribel, Eraso Barrio, Elena, Espinel-Ingroff, Ana, Ezpeleta Lobato, Guillermo, Fernández Rodríguez, Manuel, Finquelievich, Jorge Luis, García Rodríguez, Julio, García Ruiz, Juan Carlos, Giusiano, Gustavo, Guarro Artigas, Josep, Guinea Ortega, Jesús Vicente, Hernández Almaraz, José Luis, Hernández Rodríguez, César, Jarque Ramos, Isidro, Jauregizar Albonigamayor, Nerea, Linares Sicilia, María José, López Ribot, José Luis, López Romero, Everardo, López Soria, Leyre Mónica, Marcos Arias, Cristina, Martín Mazuelos, Estrella, Mayayo Artal, Emilio, Mendoza, Leonel, Montejo Baranda, Miguel, Moragues Tosantos, María Dolores, Negroni Briz, Ricardo, Olaechea Astigarraga, Pedro María, Santos Pais, Célia, Pemán García, Francisco Javier, Pereiro Ferreirós, Manuel, Rementeria Ruiz, Aitor, Rezusta López, Antonio, Ribacoba Bajo, Laureano, Rivas Pinedo, Pilar, Rodríguez Iglesias, Manuel, Salavert Lletí, Miguel, Sánchez Reus, Ferran, Severo, Luiz Carlos, Sevilla García, María Jesús, Silva Vargas, Víctor, Taylor da Cunha e Mello, Maria Lucia, Torres Rodríguez, Josep María, Villa Tanaca, Lourdes, Zaragoza Crespo, Rafael, and Zenteno Galindo, Edgar

Published
2015
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137. Oral Candida Isolates Colonizing or Infecting Human Immunodeficiency Virus-Infected and Healthy Persons in Mexico

Author

Sánchez-Vargas, Luis Octavio, primary, Ortiz-López, Natalia Guadalupe, additional, Villar, María, additional, Moragues, María Dolores, additional, Aguirre, José Manuel, additional, Cashat-Cruz, Miguel, additional, Lopez-Ribot, Jose Luis, additional, Gaitán-Cepeda, Luis Alberto, additional, and Quindós, Guillermo, additional

Published
2005
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140. Multiple Patterns of Resistance to Fluconazole in Candida glabrata Isolates from a Patient with Oropharyngeal Candidiasis Receiving Head and Neck Radiation

Author

Redding, Spencer W., primary, Kirkpatrick, William R., additional, Saville, Stephen, additional, Coco, Brent J., additional, White, William, additional, Fothergill, Annette, additional, Rinaldi, Michael, additional, Eng, Tony, additional, Patterson, Thomas F., additional, and Lopez-Ribot, Jose, additional

Published
2003
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143. Vaccine-Mediated Immune Responses to Experimental Pulmonary Cryptococcus gattii Infection in Mice.

Author

Chaturvedi, Ashok K., Hameed, Rumanasma S., Wozniak, Karen L., Hole, Camaron R., Leopold Wager, Chrissy M., Weintraub, Susan T., Lopez-Ribot, Jose L., and Wormley Jr, Floyd L.

Subjects
LUNG infections, PATHOGENIC fungi, VACCINES, IMMUNE response, IMMUNOCOMPETENT cells, CRYPTOCOCCUS, IMMUNIZATION
Abstract

Cryptococcus gattii is a fungal pathogen that can cause life-threatening respiratory and disseminated infections in immune-competent and immune-suppressed individuals. Currently, there are no standardized vaccines against cryptococcosis in humans, underlying an urgent need for effective therapies and/or vaccines. In this study, we evaluated the efficacy of intranasal immunization with C. gattii cell wall associated (CW) and/or cytoplasmic (CP) protein preparations to induce protection against experimental pulmonary C. gattii infection in mice. BALB/c mice immunized with C. gattii CW and/or CP protein preparations exhibited a significant reduction in pulmonary fungal burden and prolonged survival following pulmonary challenge with C. gattii. Protection was associated with significantly increased pro-inflammatory and Th1-type cytokine recall responses, in vitro and increased C. gattii-specific antibody production in immunized mice challenged with C. gattii. A number of immunodominant proteins were identified following immunoblot analysis of C. gattii CW and CP protein preparations using sera from immunized mice. Immunization with a combined CW and CP protein preparation resulted in an early increase in pulmonary T cell infiltrates following challenge with C. gattii. Overall, our studies show that C. gattii CW and CP protein preparations contain antigens that may be included in a subunit vaccine to induce prolonged protection against pulmonary C. gattii infection. [ABSTRACT FROM AUTHOR]

Published
2014
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144. Epidemiology of OropharyngealCandidaColonization and Infection in Patients Receiving Radiation for Head and Neck Cancer

Author

Redding, Spencer W., primary, Zellars, Richard C., additional, Kirkpatrick, William R., additional, McAtee, Robert K., additional, Caceres, Marta A., additional, Fothergill, Annette W., additional, Lopez-Ribot, Jose L., additional, Bailey, Cliff W., additional, Rinaldi, Michael G., additional, and Patterson, Thomas F., additional

Published
1999
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148. Detection of Candida dubliniensis in Oropharyngeal Samples from Human Immunodeficiency Virus-Infected Patients in North America by Primary CHROMagar Candida Screening and Susceptibility Testing of Isolates

Author

Kirkpatrick, William R., primary, Revankar, Sanjay G., additional, Mcatee, Robert K., additional, Lopez-Ribot, Jose L., additional, Fothergill, Annette W., additional, McCarthy, Dora I., additional, Sanche, Stephen E., additional, Cantu, Rebecca A., additional, Rinaldi, Michael G., additional, and Patterson, Thomas F., additional

Published
1998
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149. In VitroActivity of Miltefosine against Candida albicansunder Planktonic and Biofilm Growth Conditions and In VivoEfficacy in a Murine Model of Oral Candidiasis

Author

Vila, Taissa Vieira Machado, Chaturvedi, Ashok K., Rozental, Sonia, and Lopez-Ribot, Jose L.

Abstract

ABSTRACTThe generation of a new antifungal against Candida albicansbiofilms has become a major priority, since biofilm formation by this opportunistic pathogenic fungus is usually associated with an increased resistance to azole antifungal drugs and treatment failures. Miltefosine is an alkyl phospholipid with promising antifungal activity. Here, we report that, when tested under planktonic conditions, miltefosine displays potent in vitroactivity against multiple fluconazole-susceptible and -resistant C. albicansclinical isolates, including isolates overexpressing efflux pumps and/or with well-characterized Erg11 mutations. Moreover, miltefosine inhibits C. albicans biofilm formation and displays activity against preformed biofilms. Serial passage experiments confirmed that miltefosine has a reduced potential to elicit resistance, and screening of a library of C. albicanstranscription factor mutants provided additional insight into the activity of miltefosine against C. albicansgrowing under planktonic and biofilm conditions. Finally, we demonstrate the in vivoefficacy of topical treatment with miltefosine in the murine model of oropharyngeal candidiasis. Overall, our results confirm the potential of miltefosine as a promising antifungal drug candidate, in particular for the treatment of azole-resistant and biofilm-associated superficial candidiasis.

Published
2015
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150. Drug susceptibility of matrix-encapsulated Candida albicans nano-biofilms.

Author

Srinivasan, Anand, Gupta, Celia Macias, Agrawal, C. Mauli, Leung, Kai P., Lopez‐Ribot, Jose L., and Ramasubramanian, Anand K.

Abstract

ABSTRACT The rise in the use of biomedical devices and implants has seen a concomitant surge in the advent of device-related nosocomial (hospital-acquired) infections of bacterial and fungal origins. The most common nosocomial fungal infection is candidiasis caused mainly by Candida albicans biofilms. Candidiasis is associated with an unacceptably high mortality rate, and there is an urgent need for the discovery of new antifungal drugs that prevent or control biofilm formation. To this end, we recently developed an ultra-high-throughput microarray platform consisting of nano-scale biofilms of C. albicans encapsulated in collagen or alginate hydrogel matrices for antifungal drug screening. Here, we report that the choice of matrix influences the apparent susceptibility of C. albicans to the common antifungal drugs, amphotericin B, and caspofungin. While amphotericin B is equally effective against biofilms grown in collagen and alginate matrices, caspofungin is effective only against biofilms grown only in alginate, but not in collagen. We demonstrate differences in the distribution of the drugs in the two matrices may contribute to the susceptibility of C. albicans nano-biofilms. In a larger context, our results highlight the importance of the choice of matrix as a parameter in 3D cell encapsulation, and suggest a screening strategy to predict drug performance in vivo. Biotechnol. Bioeng. 2014;111: 418-424. © 2013 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Published
2014
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