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Objective: This study aimed to examine drug packaging and labeling, identifying similarities among them that may lead to medication errors, which may occur by unintentional substitution, in different sectors of the pharmacy of a university hospital in northeastern Brazil., Methods: Cross-sectional observational study, which included 300 pharmaceutical presentations (150 pairs) that were photographed from May to December 2010. Concordance analysis of data related to the pictures of possibly similar packaging and labels was validated using the Kappa index., Results: Of all drugs evaluated (n = 150), about 43% of "possibly similar drugs" were in the central pharmacy (n = 65) and were related to small-volume parenteral solutions. The strength of interobserver agreement in the category "very similar to each other" was considered "satisfactory" (Kappa = 0584) in 90.66% of the drugs evaluated (n = 136). The overall Kappa analysis of the study was 0.488. Variables with statistical significance were: "same color label or packaging", with the respective percentages for both primary and secondary packaging (52%-44%), p = 0.028; the variable "same color of drug presentation" obtained similar values and statistical significance to the previous variable; for the variable "same arrangement of words", the values found for both packages were close to 50%, p = 0.001; and for the variable "same color of the words", the percentages were: (50.7% - 44%) (p = 0.008)., Conclusion: Our results indicate similarities related to the labeling of drugs with potential for errors, especially in dispensing, storage, and administration if preventive measures are not adopted.

Purpose: To explore which measures could predict the persistency of developmental language impairment (DLI) based on the association between the initial language assessment and the therapeutic prognosis of the child., Methods: In this retrospective study, the records of 42 children with diagnosis of DLI were analyzed. Participants' age varied from 21 to 63 months at the first language assessment, which included vocabulary, phonology, pragmatics and fluency tests. The performance of subjects in each test was scored from 0 to 4, based on the severity of the deficits, and the maximum score corresponded to age-adequate performance. As prognostic measure, we accounted the length of therapy (in sessions) of patients who were discharged, were referred to another service (because the deficits had become very mild), or remained in therapy (persistent language difficulties)., Results: There was association between initial assessment (normal or mild alterations for vocabulary and pragmatics abilities) and prognosis (<135 therapeutic sessions). Vocabulary was the only variable able to predict the length of therapy. Being classified as severe in this measure caused the estimate of treatment to increase, in average, 112 sessions., Conclusion: The first vocabulary assessment can contribute to predict the child's therapeutic prognosis. This finding is clinically and scientifically relevant to Speech-Language Pathology, since it offers an auxiliary resource to the prognosis and therapeutic planning in cases of DLI.

Objectives: This study verified the efficacy of a formal auditory training (AT) program in children with hearing and language disorders using the auditory brainstem response to complex sounds (c-ABR) with and without background noise., Patients and Methods: Thirty children (7-13 years) were distributed into four groups: typical development (TD: n = 7), auditory processing disorder (APD: n = 9) and specific language disorder (SLIa: n = 6, and SLIb: n = 8). All children underwent behavioral assessment of auditory processing and c-ABR with and without background noise. The APD and SLIa groups underwent 8 weeks of formal AT, but all children were reevaluated 12 weeks after the initial assessment., Results: The TD group presented better behavioral performance than the other groups. For c-ABR in silence, no significant differences were observed among groups or assessments, except regarding VA complex measures, which were altered in the APD group. For c-ABR with background noise, however, the APD, SLIa and SLIb groups presented delayed latencies. Groups that underwent formal AT improved behavioral performance and decreased latencies to c-ABR in background noise at final assessment., Conclusions: These data suggest that efficacy of formal AT can be demonstrated by c-ABR with background noise., (Copyright © 2012 S. Karger AG, Basel.)

Purpose: To verify the use of conjunctions in narratives, and to investigate the influence of stimuli's complexity over the type of conjunctions used by children with specific language impairment (SLI) and children with typical language development., Methods: Participants were 40 children (20 with typical language development and 20 with SLI) with ages between 7 and 10 years, paired by age range. Fifteen stories with increasing of complexity were used to obtain the narratives; stories were classified into mechanical, behavioral and intentional, and each of them was represented by four scenes. Narratives were analyzed according to occurrence and classification of conjunctions., Results: Both groups used more coordinative than subordinate conjunctions, with significant decrease in the use of conjunctions in the discourse of SLI children. The use of conjunctions varied according to the type of narrative: for coordinative conjunctions, both groups differed only between intentional and behavioral narratives, with higher occurrence in behavioral ones; for subordinate conjunctions, typically developing children's performance did not show differences between narratives, while SLI children presented fewer occurrences in intentional narratives, which was different from other narratives., Conclusion: Both groups used more coordinative than subordinate conjunctions; however, typically developing children presented more conjunctions than SLI children. The production of children with SLI was influenced by stimulus, since more complex narratives has less use of subordinate conjunctions.

Purpose: To investigate if the performance on linguistic tasks would be predictive of orthographic domain and quality of written productions., Methods: Participants were 82 fourth graders of Elementary Education, from public and private schools of São Paulo, with ages ranging from 9 years to 10 years and 2 months. The test battery was composed of an expressive vocabulary test, phonological awareness and rapid serial naming tasks, words and pseudowords spelling, and written text composition using a visual stimulus as a starting point. The statistical analysis included Spearman (r) correlations among all tasks., Results: The results indicated that the better the vocabulary skills, the smaller the number of spelling errors and the better the quality of the written text productions, considering all the analyzed categories. Also, the higher performance in both phonological awareness and rapid object naming tasks was correlated to fewer spelling errors and written text productions with greater grammatical structure., Conclusion: The linguistic abilities analyzed in this study were predictive of subjects' spelling performance. The vocabulary skills were predictive of the quality of written text productions. However, phonological awareness and rapid serial naming were only predictive of children's performance concerning the syntactic and grammatical structure of their written text productions.

The balance between excitatory and inhibitory synapses is crucial for normal brain function. Wnt proteins stimulate synapse formation by increasing synaptic assembly. However, it is unclear whether Wnt signaling differentially regulates the formation of excitatory and inhibitory synapses. Here, we demonstrate that Wnt7a preferentially stimulates excitatory synapse formation and function. In hippocampal neurons, Wnt7a increases the number of excitatory synapses, whereas inhibitory synapses are unaffected. Wnt7a or postsynaptic expression of Dishevelled-1 (Dvl1), a core Wnt signaling component, increases the frequency and amplitude of miniature excitatory postsynaptic currents (mEPSCs), but not miniature inhibitory postsynaptic currents (mIPSCs). Wnt7a increases the density and maturity of dendritic spines, whereas Wnt7a-Dvl1-deficient mice exhibit defects in spine morphogenesis and mossy fiber-CA3 synaptic transmission in the hippocampus. Using a postsynaptic reporter for Ca(2+)/Calmodulin-dependent protein kinase II (CaMKII) activity, we demonstrate that Wnt7a rapidly activates CaMKII in spines. Importantly, CaMKII inhibition abolishes the effects of Wnt7a on spine growth and excitatory synaptic strength. These data indicate that Wnt7a signaling is critical to regulate spine growth and synaptic strength through the local activation of CaMKII at dendritic spines. Therefore, aberrant Wnt7a signaling may contribute to neurological disorders in which excitatory signaling is disrupted.

Objective: To determine the clinical and epidemiological profile of patients who are candidates for TNF-α inhibitor use and are classified as having latent tuberculosis (LTB), as well as to evaluate the outcomes of prophylactic treatment with isoniazid., Methods: A prospective descriptive analysis followed by an analytical, observational, cross-sectional study of the outcomes of prophylactic treatment in a group of 45 candidates for TNF-α inhibitor use. We evaluated the patients through anamnesis, clinical examination, chest X-ray, and tuberculin skin test (TST) using the Mantoux method., Results: The mean age was 45 years, and 56.0% of the patients were female. Chronic rheumatic diseases, chronic dermatological diseases, and Crohn's disease were present in 46.7%, 40.0%, and 13.3% of the patients, respectively. The mean TST induration was 14.6 mm (range: 5-30 mm). The majority (n = 30) of the 45 patients (66.7%) had an induration > 10 mm. In the 16 patients with BCG vaccination scars, the mean induration was 15.7 mm, and 14 of those patients had an induration > 10 mm. Chest X-ray results were considered normal, with minimal alterations, in 64.4% and 35.6% of the patients, respectively. The treatment with isoniazid was abandoned by 1 patient (2.2%) and completed by 41 (91.2%), whereas it was interrupted because of drug-induced hepatitis in 2 (4.4%), and 1 patient (2.2%) was transferred to another hospital. Of those who completed the treatment, 5 experienced mild side effects., Conclusions: Determining the profile of candidates for TNF-α inhibitor use is important for the management of LTB treatment and for the establishment of clinical protocols for the use and monitoring of the use of these medications.

Purpose: To verify whether the use of a shorter filmed sample influences the results of pragmatic assessment in children with language impairment., Methods: Data was obtained from videotaped samples and protocols for pragmatic analysis of 30 children between 3 and 6 years old. Each 15-minute sample (total moment) was analyzed and divided into three moments with five minutes each (initial, medial and final moments). For data comparison, the number of communicative acts per minute, the percentage of communicative space, the predominant communicative functions and the percentage of each communicative mean (gestural, vocal and verbal) were registered in all moments., Results: No differences were found between moments for number of communicative acts, communicative space used and communicative functions used. Regarding communicative functions, in all moments the most recurrent were Comment, Information Request and Performative, and the least frequent were Request for Social Routine, Non-Focused and Narrative. Regarding communicative means, a difference was found between initial and final moments (p=0.048) and a tendency of difference was found between initial and medial moments (p=0.097) only in the use of the gestural mean., Conclusion: Pragmatic assessment in children with language impairment can be reduced five-minute samples, as long as the first minutes are discarded and the medial or final minutes are used for analysis.

Background: The narrative abilities provide valuable information about the linguistic, cognitive and social development of school-age children with typical language development (TLD)., Aim: To examine the temporal ordering of figures and the narrative abilities of school-age children with TLD in relation to the type of discourse presented., Method: Participants of this study included 60 children with TLD aged between seven and ten years of age. Fifteen stories were used in the study. Each story was illustrated by four figures. The sequences of figures were created and classified as mechanical, behavioral and intentional according to the relationships presented among the characters. Data were transcribed and analyzed regarding the type of discourse (descriptive, causal and intentional) and the type of organization of the figures., Results: No differences between age groups were observed for temporal ordering. For all age groups, the most frequently presented discourse type was the causal one. Statistically significant differences were observed among age groups for the causal and intentional discourse type. Inasmuch as the age increased, school-age children with TLD reduced the use of the descriptive discourse and increased the use of the intentional one., Conclusion: The ability of temporal ordering is already developed in children with TLD at seven-years of age. The type of discourse was influenced by age and by the type of story presented.

Background: The Auditory Nerve Recovery Function (REC) may be extracted from the Electrically Evoked Compound Action Potential (ECAP). ECAP may be influenced by the stimulation received (or the deprivation of stimulation) and by the etiology of the hearing loss, consequently it might affect the REC., Aim: To verify whether there is a correlation between the REC and each of the following factors: etiology, time of auditory deprivation and time of hearing aid use before cochlear implantation (CI)., Method: Retrospective study. Data regarding etiology, time of auditory deprivation, time of hearing aid use before cochlear implantation were collected in children and adults who received a Nucleus®24. All patients who presented neural response at surgery and whose REC was assessed intraoperatively were included in this study. Fifty patients were selected, 26 children and 24 adults. Patients were divided according to the REC classification into three groups (GI: fast recovery; GII: intermediate recovery and GIII: slow recovery) to allow correlation analysis., Results: Data analysis did not show any statistically significant correlation between the recovery function and the pre-implant studied characteristics. Nevertheless, it was observed that there was a greater concentration of both, children and adults, in the intermediate recovery function values. GI did not present individuals with infectious etiologies, such as meningitis, rubella and cytomegalovirus. REC average scores were slower in infectious etiologies for both children and adults., Conclusion: There was no statistically significant correlation between the recovery function and factors such as etiology, time of auditory deprivation and time of hearing aid use prior to CI.

Background: children with Specific Language Impairment (SLI) have difficulties with speech processing. These difficulties affect the development of phonologic representations., Aim: to evaluate the abilities of children with normal language development (NLD) and those with SLI in distinguishing words from non-words in a lexical decision task., Method: two groups were involved in this study: the Control Group (GC), with no language disorders, composed by 36 subjects, and the Research Group (RG), with 18 subjects, all diagnosed with SLI, aging form 4 to 8:9 years. Children from both groups were arranged in three subgroups, according to the receptive vocabulary. Forty eight three syllable words were selected, being 24 real words and 24 that were manipulated in order to obtain non-words. Three variables were considered: (a) modification extension, (b) modification positioning and (c) modification type. Children had to decide whether a phonological sequence consisted of a word or a non-word., Results: even though children were matched by lexical age, there were differences between GC and RG. The RG presented more difficulty in lexical decision, not only for words but also for non-words. Both groups, with lexical age of 4 years, struggled more in this task when compared with groups with lexical age of 5 and 6 years., Conclusion: children with SLI presented deficit in phonological representation when compared with children with NLD. This difference in performance can be explained by differences in the formation and retention of working memory representations, auditory discrimination and motor planning and execution.

Background: syllable deletion (SD) frequently occurs in Specific Language Impairment and can indicate a deviant factor in the phonologic acquisition process of these subjects., Aim: to verify the occurrence of SD in the spontaneous speech of children with SLI and to verify the influence of word extension and syllable stress in this process., Methods: participants were 27 children with SLI, aged between 3:0 and 5:11 years, in a weekly speech treatment, who presented 50% of correct answers in specific phonology assessment tasks or who presented speech intelligibility within levels that allowed assessment through spontaneous speech. Speech samples were obtained during a play interaction situation between the researcher and the child and through speech elicited by the presentation of a picture. The occurrence of SD was analyzed considering the following parameters: extension of the produced words, preference for stressed or unstressed syllables, position of the syllable within the word were SD occurred., Results: there was a preference for the production of dissyllabic words (X2 = 72,49; p < 0,001); the occurrence of SD was significantly higher in polysyllabic words (X2 = 11,22; p < 0,004) and on initial syllables (X2 = 34,99; p < 0,001). Unstressed syllables were more often reduced (Z= -5.79, p <0001)., Conclusion: the preference for the production of dissyllabic words reassures the difficulty of these children with complex syllabic structures and, in part, explains their spontaneous speech unintelligibility. The predominance of unstressed syllable deletion indicates the preference for producing the nucleus of words, where emphasis is given to the stressed syllable during language expression.

Background: children with Specific Language Impairment (SLI) show lexical deficits as the first noticeable sign of such disorder, characterized as difficulties in lexical access during naming and speech tests. Studies that compare picture naming and drawings seem perfect to clarify lexical deficits., Aim: to compare the performance of children with normal language development (NLD) to that of children with SLI in naming, drawing and definition tasks, aiming to explore the the quality of semantic representation of the lexicon., Method: Two groups were involved in this study: the Control Group (CG), with no language disorders, composed by 40 subjects, and the Research Group (RG), with 20 subjects, all diagnosed with SLI, aging from five to seven years. Tasks of naming, picture drawing and definition were performed, using 20 different pictures. In the naming task, the types of errors were analyzed and sorted as follows: semantic, phonological, none specified and others. The analysis of the drawing and definition tasks was based only on the correct answers, semantic and none specified errors., Results: children of the RG presented a greater number of semantic errors in the picture naming task when compared to the CG. Besides that, definitions presented by the RG seemed more simple and incomplete even when the child was capable of naming the picture correctly. Drawings of correctly named objects were better than those that were named incorrectly., Conclusions: it was possible to discriminate within SLI children those that present greater lexical deficits. It was also possible to explore the possible reasons for failures in naming tasks.

Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed heterozygosity in M. rufiventris and M. mondury, thereby reinforcing the idea that populational studies should be undertaken by preferably using species-specific microsatellite primers.

Diversity is one of the major characteristics of Brazil and all South America. This paper presents an overview of the current situation of the education of speech and language pathologists (SLP) and audiologists in Brazil and in several other countries of South America. This paper also discusses the main challenges shared by these countries. The discussion is focused on the mutual interferences between education and the areas of professional practice, cultural diversity and continued education. There are many emerging issues about the education of SLP and audiologists in South America. The suggested conclusion is that, despite the many differences, the South American SLP and audiologists' education would benefit from joint efforts and collaborative experiences., (2010 S. Karger AG, Basel.)

Background: verb acquisition., Aim: to verify quantitative and qualitative verb diversity in the spontaneous speech of Brazilian Portuguese-speaking preschoolers and verb acquisition from 2 to 4 years of age., Method: participants were sixty preschoolers equally matched on gender and age, divided into three groups: GI (2-year-old children), GII (3-year-old children) and GIII (4-year-old children). Spontaneous speech samples obtained through playing interactions, in educational contexts, were collected, and verb productions were listed., Results: children used 167 different verbs. The quantitative analysis indicated significant statistical differences between all groups (p< .001), with gradual increase of verb usage from 2 to 4 years of age. However, no statistical difference (p= .956) was observed between genders., Conclusion: preschoolers improved their use of verbs during the initial stages of language acquisition, irrespective of their gender.

Background: Several studies suggest the association of specific language impairment (SLI) to deficits in auditory processing. It has been evidenced that children with SLI present deficit in brief stimuli discrimination. Such deficit would lead to difficulties in developing phonological abilities necessary to map phonemes and to effectively and automatically code and decode words and sentences. However, the correlation between temporal processing (TP) and specific deficits in language disorders--such as syntactic comprehension abilities--has received little or no attention., Aim: To analyze the correlation between: TP (through the Frequency Pattern Test--FPT) and Syntactic Complexity Comprehension (through a Sentence Comprehension Task)., Method: Sixteen children with typical language development (8;9 +/- 1;1 years) and seven children with SLI (8;1 +/- 1;2 years) participated on the study., Results: Accuracy of both groups decreased with the increase on syntactic complexity (both p < 0.01). On the between groups comparison, performance difference on the Test of Syntactic Complexity Comprehension (TSCC) was statistically significant (p = 0.02).As expected, children with SLI presented FPT performance outside reference values. On the SLI group, correlations between TSCC and FPT were positive and higher for high syntactic complexity (r = 0.97) than for low syntactic complexity (r = 0.51)., Conclusion: Results suggest that FPT is positively correlated to syntactic complexity comprehension abilities.The low performance on FPT could serve as an additional indicator of deficits in complex linguistic processing. Future studies should consider, besides the increase of the sample, longitudinal studies that investigate the effect of frequency pattern auditory training on performance in high syntactic complexity comprehension tasks.

In the search for an efficient nonviral gene therapy approach for the treatment of genetic disorders of cardiac and skeletal muscle such as Duchenne muscular dystrophy, ultrasound in combination with contrast enhancing microbubbles has emerged as a promising tool for safe and site-specific enhancement of gene delivery. Indeed, microbubble-enhanced gene transfer (MBGT) has been investigated for a wide variety of target sites using both reporter and therapeutic genes. Although a range of different microbubbles have been used for MBGT studies, comparison of their efficiencies is difficult because microbubble concentration and the ultrasound settings used for the application vary considerably. Only two studies to date have attempted a direct comparison of commercially available microbubbles, and both concluded that not all microbubbles show the same efficiencies with MBGT. Thus far, the reason for this is unclear. Here, the efficiency of three commercially available microbubbles--Optison, SonoVue and Sonazoid--was analyzed to understand the microbubble properties that are important for their function as an effective enhancer for gene transfer in vivo. In this study, plasmid DNA or antisense oligonucleotides were delivered by systemic injection with MBGT, focused on the heart. Gene delivery to the heart with equalized concentrations of the three microbubbles showed that Optison and Sonazoid are more efficient in MBGT compared with SonoVue, which showed the weakest gene transfer to the myocardium. Investigations into the properties of these microbubbles showed that size and shell composition did not directly influence MBGT, whereas the microbubbles with increased stability in an ultrasound field showed better MBGT results than those degrading faster. Moreover, the microbubble concentration used for MBGT was also found to be an important factor influencing the efficiency of MBGT. In conclusion, the stability of a microbubble was shown to be a major influential factor for its performance in MBGT, as is the concentration of the microbubbles used. These findings emphasize the importance of detailed investigations into the properties of microbubbles to allow the production of a microbubble specifically designed for optimum performance with MBGT.

Eight microsatellite primers were developed from ISSR (intersimple sequence repeats) markers for the stingless bee Melipona rufiventris. These primers were tested in 20 M. rufiventris workers, representing a single population from Minas Gerais state. The number of alleles per locus ranged from 2 to 5 (mean = 2.63) and the observed and expected heterozygosity values ranged from 0.00 to 0.44 (mean = 0.20) and from 0.05 to 0.68 (mean = 0.31), respectively. Several loci were also polymorphic in M. quadrifasciata, M. bicolor, M. mandacaia and Partamona helleri and should prove useful in population studies of other stingless bees., (© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd.)

Background: studies about Brazilian Portuguese number morpheme acquisition and its productive usage have indicated controversial results., Aim: to verify the comprehension and production of singular and plural markers in children within normal language development., Method: participants were 64 children ranging in age from 3:0 to 6:11 years. In the comprehension test, children were encouraged to point to the correctly inflected noun picture, among three foils. In the production test, they had to name each picture with the correctly inflected article and noun., Results: there was an increase of correct answers with age. The production of the plural form received the lower scores, but presented a significant enhancement from 3:0 to 5:0 years., Conclusion: this ability improved with development and was considered productive after 5:0.

Background: Phonological working memory and typical language development in children., Aim: To describe and discuss findings of researches concerning the assessment of phonological working memory in normally developing children undertaken since the 1980's., Aim: To present a systematic literature review regarding the phonological working memory and its relationship with normal language development., Method: A systematic review of the literature on phonological working memory and its relationship with language development skills of normal children was made. The used material involved books, monographs, thesis, dissertations and articles stored in Lilacs, Pubmed, Scielo and Medline databases. An analysis was made considering the applied tests and the results regarding the effects of extension and age and the relationship of these variables with phonological working memory for speakers of English and the Brazilian Portuguese language., Conclusion: According to the consulted literature, studies indicate a relationship between the phonological and lexical knowledge and the phonological working memory in children with normal language development.

Background: translation and cross-cultural adaptation of instruments to the Brazilian Portuguese language., Aim: in Brazil, in the area of Speech-Language Pathology, there is a significant lack of commercially available formal and objective instruments that have as a purpose the evaluation and diagnosis of different communication disorders. Some researchers have tried to ease this problem by translating instruments available in other languages. Such procedure also contributes to the development of cross-cultural studies, which can bring knowledge about the communication disorders and their specificities in different languages. The aim of the present study is to discuss the procedures that should be adopted in this process., Conclusion: it is fundamental to adopt appropriate methodological procedures when translating and adapting foreign assessment instruments.

Background: narrative abilities provide rich information about the linguistic, cognitive and social competences of children with typical language development and with SLI (Specific Language Impairment). Children with SLI present deficits in speech elaboration, which is generally confusing and repetitive. Furthermore, there may be difficulty with text organization, understanding the underlying time and cause-effect relationships and in the development of the structural knowledge needed for comprehension., Aim: to characterize the narration of stories by children with SLI regarding the type and content of speech and to compare their performance to that of their typically developing peers, matched according to the chronological age., Method: two groups participated in this study: Control Group (CG), 24 children with no language deficits and Research Group (RG), 8 children with the diagnosis of SLI. To elicit the narratives, a series of 15 stories were used, represented by illustrations containing four scenes each. These sequences were created and classified as mechanical, behavioral and intentional, according to the relationship established between the characters., Results: children with SLI presented poorer narratives when compared to their typically developing peers, independent of the type of story which was presented. Moreover, children with SLI showed a similar perception of the mental states when compared to children with normal development., Conclusions: these results indicate that, regardless the type of story, children with SLI have difficulties in the use of language, that is, with the linguistic abilities necessary to narrate stories and not in the perception of the characters' mental state.

Background: application of an American early language test in Brazilian Portuguese-speaking subjects., Aim: to compare the performance of normal Brazilian Portuguese and American English-speaking subjects on the Test of Early Language Development - Third Edition (TELD-3)., Method: participants of this study were 120 subjects of both genders, ranging in age from 2:00 to 7:11 years (20 subjects in each age range). Subjects were selected and tested individually by the researcher. A comparative analysis of the subjects' performances with that observed in English-speaking children was made., Results: the results indicated that Brazilian and American subjects presented similar performances until the age of 4:11 years. Portuguese-speaking children of 5, 6 and 7 years presented higher scores on the receptive subtest of Form A when compared to their American pairs. Portuguese-speaking subjects of 5 and 6 years presented lower scores on the expressive subtest of Form B when compared to their American pairs., Conclusion: the translation of diagnostic instruments that are already available in other languages can lessen the lack of such resources in countries where these instruments are not yet available. Furthermore, this practice can allow cross-cultural studies which are relevant for researches on child language and language disabilities.

Background: relationship between Specific Language Impairment (SLI) and visual short-term memory., Aim: to compare the performance of children with SLI to a control group of children with normal language development in tasks involving visual short-term memory., Method: subjects were 20 SLI children (ages 3;0 to 5;11), and 29 children with normal language development (ages 2;0 to 4;11), assessed in tasks of visual short-term memory involving picture recognition and localization recall of objects previously manipulated by the examiner. As the diagnosis of SLI implies in the linguistic age being at least one year below the expected for the chronological age, the control group was also constituted by younger children with normal language development., Results: SLI children presented an inferior performance when compared to their pairs of the same age, similar to the younger children or below the younger age group., Conclusion: SLI children presented deficits in tasks involving visual short-term memory, which must be discussed in order to understand the nature of the disorder and also in terms of speech-language intervention.

Background: children start to differentiate questions from non-questions and also start to adjust their answers at the age of two. This participation in verbal exchanges requires basic conversational abilities such as the competence to initiate interact and reply appropriately, and to maintain the interaction., Aim: to analyze and correlate the pragmatic aspects of language, related to the type of answers, during an adult/child interaction in children with normal language development and in those diagnosed with Specific Language Impairment (SLI)., Method: 16 children with SLI (GP) and ages between three and six years, and 60 children with normal language development (GC) and ages between three to five years - 20 for each age group, 10 of each gender. Data gathering occurred in two different days, with the adult/child interaction being facilitated by toys. Data (speech of child and adult) were transcribed and analyzed, obtaining a Reliability Index of 93.75%, and later were submitted to statistical analyses., Results: answers were classified in categories and grouped according to the following: Adequate Answers (RA) and Inadequate Answers (RI), always in accordance to the established communicative context. GC presented a significantly higher average of RA when compared to GP, and GP presented a significantly higher average of RI when compared to GC. GC presented a decrease in the use of RI at the increase of age. GP maintained the use of RI at the increase of age, including the age group of six., Conclusion: it was observed that the increase in age emphasized differences between GP and GC. GP presented a less effective communication maintaining unintelligible speech, whereas GC presented more elaborate communication abilities. More studies with older children are necessary for the better understanding of the observed trends.

Anxiety- and depression-related disorders often appear associated and may be affected by common genetic factors. The inbred rat strains Lewis (LEW) and spontaneously hypertensive rats (SHR) and the outbred rat lines Floripa H and L, which were selectively bred for high and low locomotion in the central area of the open field (OF) test, respectively, have been proposed as experimental tools to study anxiety. The main goal of the present study was to characterize the behavior of these animals in two models of anxiety, elevated plus-maze (EPM) and OF, in two models of depression, forced swim test (FST) and tail suspension test (TST) and in their home-cages. Emotionality-related differences between LEW and SHR rats and between Floripa H and L rats were found in the EPM, OF and FST. Those lines showing low anxiety-like profiles in the EPM and OF (SHR and Floripa H) also showed low immobility in the FST. The TST failed to unveil any line differences. Factor analysis involving all tests revealed three independent factors with one of them associating anxiety-related measures from the OF and EPM to immobility in the FST. When observed in their home-cages, LEW and SHR rats showed no differences in general activity, but when acutely treated with imipramine (15mg/kg), only LEW rats were sensitive to its antidepressant effects. These results suggest the existence of a genetic link between two tests used in the screening of anxiolytic drugs and one test of antidepressant activity. Moreover, the LEW and SHR rat strains were shown to be an interesting model to study the comorbidity between anxiety- and depression-related disorders.

The behavioral characterization of rodent strains in different studies and laboratories can provide unreplicable results even when genotypes are kept constant and environmental control is maximized. In the present study, the influence of common laboratory environmental variables and their interaction with genotype on the results of behavioral tests of anxiety/emotionality were investigated. To this end, the inbred rat strains Lewis (LEW) and spontaneously hypertensive rats (SHR), which are known to differ for numerous emotionality-related behaviors, were tested in the open field (OF), elevated plus maze (EPM) and black/white box (BWB), while three environmental factors were systematically controlled and analyzed: (1) the experimenter handling the animal (familiar or unfamiliar); (2) the position of the home cage (top or bottom shelf of the rack) and (3) the behavioral state of the animal immediately before the test (arousal or rest). Experimenter familiarity did not alter the behavior of rats in the OF. Cage position, on the other hand, influenced the behavior in the OF and BWB, with rats housed in top cages appearing less anxious than those housed in the bottom. In the BWB (but not in the OF), these effects were genotype dependent. Finally, the behavioral state of the animals prior to testing altered the results of the EPM in a strain-dependent manner, with some anxiety-related genotypic differences being found only among rats that were aroused in their home cages. This study showed that common variations in the laboratory environment interact with genotype in behavioral tests of anxiety/emotionality. Recognizing and understanding such variations can help in the design of more effective experiments.

The role of pair housing in the modulation of anxiety-like behaviour in socially and physiologically stressed mice was investigated. The protocol of psychosocial stress consisted of submitting male adult mice to daily social confrontation with a male conspecific for a period of thirteen days. In an attempt to study a possible effect of pair housing as a social support, each male mouse was housed with a female throughout the period of experimentation, except during the agonistic interactions. As a physiological stressor, 10(9) sheep red blood cells (SRBC)/ml were injected intraperitoneally on the 1st and 7th days of the experiments. The respective control groups were as follows: non-socially stressed, non-pair housed and saline-injected mice. The humoral immune response was analysed by haemagglutination assay. The level of anxiety-like behaviours was measured in the elevated plus-maze test on the 13th day of the experiment. As a result, no significant changes in humoral immunity to SRBC were observed in mice subjected to social confrontation in a neutral arena as compared to non-socially stressed mice. As a consequence, no effect of pair housing on humoral immunity to SRBC could be evaluated. Concerning the effects of pair housing on the anxiety-like behaviours, it was possible to demonstrate that the pair housing proved to be effective in modulating anxiety-like behaviour, although in the stressed groups the percentage of time in the open arms and the time in risk assessment did not change in a symmetrical opposite form, as expected. The physiological stressor induced an anxiety-like behaviour that was not reversed by the pair housed condition. This suggests that different types of stressors activate different neural and peripheral pathways, which may or may not be modulated by pair housing, a finding that deserves our attention as a way to better understand the mechanisms that influence adaptations to stress.

Background: A follow-up study of an individual with Specific Language Impairment., Aim: To describe the oral and written language development of an adolescent during 13 years of intervention., Method: A Male subject, 16 years old, in the second year of high school, who was assessed during the years with different language and auditory procedures., Results: The Subject still presents oral and written language deficits, but these do not compromise intelligibility in any level., Conclusion: The studied subject presented significant language development during the therapeutic process. However, corroborating with other literature data, deficits still remain.

Background: The ability of children with Specific Language Impairment (SLI) to use and comprehend closed-class words., Aim: Acknowledging the increasing search for the characterization of linguistic performance of this population in different languages, as well as the findings of international literature that stress the difficulty of these children in using closed-class grammatical morphemes, the aim of this study was to analyze the performance of children with SLI in tests of production and comprehension of prepositions. The purpose of this research was also to compare the performance of these children to that of their typical language developing peers, in terms of the overall index of correct answers and the most frequent types of errors in both groups., Method: An objective test was developed in order to analyze production and comprehension of four types of prepositions that are used to establish the following semantic relations: with (company relation); by (way relation); in, on or at (place/position relations); and to (destination relation). This test was carried out with 19 SLI children and 29 typically developing children (CG--control group), one year younger than the research group., Results: SLI children presented the same pattern of results, although delayed, of children in the CG in the comprehension test. In the task involving production, however, the performance of SLI children was quantitatively and qualitatively inferior to their typical developing controls. This performance was mainly characterized by the omission of prepositions., Conclusion: The findings corroborate the view that grammatical words constitute a challenge for SLI children, since they presented many errors that suggest difficulties in the domain of prepositions. The results also emphasized, for the SLI group, deficits in the expressive language.

Background: Specific language impairment., Aim: To establish relations between the performance of subjects in phonologic and pragmatic tests., Method: Tabulation and analyses of the data of phonologic and pragmatic tests of 20 children with ages raging from 3:0 to 5:11 years, with the diagnoses of Specific Language Impairment (SLI)., Results: The statistic analyses showed good correlations between developmental processes (DP) and non-developmental processes (NDP), DP and the use of verbal mean, vocal mean and number of communication acts per minute., Conclusion: The performance in the phonological and pragmatic tests does not seem to have a direct correlation in subjects with specific language impairment.

Background: to investigate the pragmatic difficulties presented by children with specific language impairment and to verify how these deficits influence their discourse performance., Aim: to analyze the request-response sequences in the discourse of normal and Specific Language Impairment Children (SLI) during a 20-minute free-play situation with an adult., Method: subjects of this study were 29 children, male and female, with ages ranging from 2:0 to 4:11 years. Data was collected during a free-play situation with an adult., Results: SLI children frequently ignored the questions made by the adult and responded innappropriately more often., Conclusion: qualitative and quantitative differences in the pattern of responses and in the use of pragmatic abilities were found for the SLI children.

8 patients with bone marrow failure after a caesium-137 radiation accident were treated with recombinant human granulocyte-macrophage colony stimulating factor (rHuGM-CSF). The 7 who were evaluable had prompt increases in granulocytes and bone marrow cellularity. 2 patients died of radiation toxicity and haemorrhage and 2 of bacterial sepsis acquired before the start of rHuGM-CSF treatment. 4 patients survive, including 2 who were treated early and never became infected. This therapeutic approach to radiation-induced granulocytopenia may therefore be useful after radiation and nuclear accidents.