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In today’s rapid development of information technology, social media has become an indispensable part of the daily communication of students in colleges and universities. The issue of student mental health is increasingly receiving widespread attention from society. As an essential position for mental health education, how to effectively utilize big data technology for students’ psychological nurturing and enhance the pertinence and effectiveness of mental health education has become an important issue in education. In this article, by creating a four-level psychological parenting model built on a positive psychology perspective, we study extensive data mining, deep learning and other methods to analyze students’ social media usage data and identify students’ mental health status. For the 225 students in this article, the anxiety and depression self-assessment scale scores decreased significantly (P

We collect Chinese A-share listed companies from 2013 to 2022 as samples and use the multi-period difference-in-difference model (DID) to study the impact of multilingual ESG report disclosure on the enthusiasm of foreign investors. We find that Chinese companies disclose ESG reports in both Chinese and English stimulate the enthusiasm of foreign investors to hold shares. The main manifestations are the expansion of the company's foreign shareholding quota and the increase in the number of shareholders. Further research show that disclosure of multilingual ESG reports makes up for the readability of company annual reports for foreign investors. In the case of companies with poor analyst attention and comparability of accounting information, and companies that hire non-big four auditing firms to audit financial reports, multilingual ESG report disclosures are more positive for foreign shareholdings. The participation of the central investor service center in corporate governance is weak, the degree of regional cultural integration is low, and the disclosure of English ESG reports by Chinese enterprises is conducive to promoting the enthusiasm of foreign shareholding. The research conclusions provide theoretical guidance and empirical reference for enterprises to expand information disclosure methods to foreign investors and attract overseas capital investment.

As an emerging art form, the development trend and impact of digital media art have attracted much attention, and the introduction of CiteSpace tools provides a new perspective for digital media art research, making it possible to analyze the scientometrics of this field. Relevant documents in Web of Science database were selected and analyzed by CiteSpace version 5.6.R4 for research collaboration and thematic co-occurrence networks. The results show that between 2004 and 2022, the total number of digital media arts research literature is 359, of which the literature volume from 2014 to 2022 accounts for 66.85%, and the average annual number of publications is about 27, reflecting the rapid growth of research in this field. Keyword analysis shows that “digital media art”, “digital technology”, “cultural industry”, “education “ and so on have become research hotspots. Digital media art research is gradually shifting from technology and nature exploration to university discipline construction and application practice, showing the trend of cross-border cooperation of disciplines.

There's a lot of uncertainty in the global financial system due to trade, political, and unforeseen issues. There are dangers and uncertainties in China's domestic financial market due to the ongoing effect of the global economic climate and the impact of the new coronavirus pandemic. Nonetheless, the conventional financial sector, which is controlled by commercial banks, continues to have a dominant position in China's financial system, with the traditional credit business model serving as its primary business model. As a result, the primary subject of this essay is commercial banks, a common kind of financial organization. The combination of the Malmquist Index Model with the new network DEA model takes into account the real circumstances faced by Chinese commercial banks. The three phases of the credit business operation process of commercial banks are capital buildup, loan issuing, and profit generation. To observe the effects of recent domestic market uncertainties and the high risk of emergencies like the epidemic on traditional financial institutions dominated by banks, this paper measures the efficiency of credit business of 26 listed banks in China from 2016 to 2022 and analyzes the operational efficiency of credit business of Chinese commercial banks from both static and dynamic perspectives. As a result, Chinese commercial banks and other established financial institutions are better equipped to reduce risks and uncertainties and support the steady growth of the country's financial sector.

Objective DJ-1 is an oncoprotein secreted by cancer cells. However, the physiological and pathological significance of DJ-1 secretion is not clearly understood. This study investigated the clinical value of serum DJ-1 in lung adenocarcinoma (LUAD). Methods The study involved 224 LUAD patients, 110 patients with benign pulmonary disease and 100 healthy controls from the First Affiliated Hospital of Nanjing Medical University. We detected the expression of DJ-1 in lung cell lines in vitro. Meanwhile, serum concentrations of DJ-1, carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and cytokeratin 19 fragment (CYFRA21-1) were measured. The diagnostic performance of LUAD was obtained using receiver operating characteristic (ROC) curves. Kaplan–Meier, univariate and multivariate Cox regression analyses were performed for progression-free survival (PFS). Results DJ-1 was highly expressed in LUAD cell lines. Serum DJ-1 levels were significantly higher in the LUAD group compared to the benign pulmonary disease group (5.04 vs. 3.66 ng/mL, P

This paper firstly classifies the ideological and political education course field dimensions, studies the specific composition of the learning field in three dimensions: information, technology and emotion, and discusses the necessity of learning field optimization in secondary school Civics class. Secondly, on the multivariate data chain network, the MIFS model is proposed to extract the ideological and political teaching feature values, followed by the LSTM neural network to train the information related to students’ Civics classroom contents and the average function of adaptive excitation function is used to replace the excitation function in the LSTM neural network to achieve the effect of improving the output accuracy. Then, the characteristics of learners and learning resources are parametrically represented, followed by an experimental analysis of ideological and political education based on multivariate data chain networks using evaluation indexes based on the data set. The results show that there is no difference between the MAE values of CNN and LSTM except for sample one, but the MSE values of LSTM are both lower than CNN, and LSTM is more stable than CNN, indicating that it is reasonable to choose LSTM when ideological and political education based on multivariate data chain network. This study provides a reference and reference for future field optimization in ideology and political science classes.

Insulation is considered the weakest link in electrical equipment. The combined effects of special working conditions in coal mines and aging factors such as electrical, thermal, and mechanical stresses make it difficult to determine the aging mechanism and evaluate the condition of EPDM insulation for high-voltage cables used in mines. This paper introduces the basic performance and aging factor types of EPDM insulation for high-voltage mobile flexible cables used in coal mines. Based on the physical, chemical, mechanical, and electrical properties of EPDM under the influence of multiple aging factors, the aging mechanism of EPDM is proposed. This paper summarizes the basic principles and existing problems of online monitoring methods for insulation resistance, partial discharge, dielectric loss factor, and temperature of mining high-voltage cables. The paper summarizes the current research status of insulation status evaluation methods for mining high-voltage cables. The paper introduces the evaluation methods for insulation status of multi parameter based on improved radar map and single parameter based on dielectric loss mining high-voltage cables. To cope with the development of coal mine intelligence, on the one hand, it is suggested to do research on intelligent perception and control of mining electrical equipment to compensate for the lack of state perception and state evaluation feature quantities. On the other hand, it is necessary to study lightweight models or algorithms to reduce the computational complexity, parameter quantity, and analysis time of intelligent terminals near devices. It improves the feasibility of state evaluation technology, and lays the foundation for achieving intelligent analysis and decision-making.

The mechanism of extracellular matrix metalloproteinase inducer (EMMPRIN) in the regulation of liver fibrosis has not been clarified. This study aims to investigate the role of EMMPRIN S-nitrosylation (SNO) in the regulation of hepatic stellate cell (HSC) migration and matrix metalloproteinase (MMP) activities in liver fibrosis. The results from the tissue microarrays and rat/mouse liver tissues suggest that EMMPRIN mRNA and protein levels in the fibrotic livers are lower than those in the corresponding normal control livers, but higher SNO level of EMMPRIN in fibrotic liver area was shown by immunohistochemistry, immunofluorescence staining, and biotin-switch assay conversely in vivo. Primary EMMPRIN comes from hepatocytes and liver sinus epithelial cells (LSECs) rather than quiescent HSCs. To mimic the uptake of extrinsic EMMPRIN, supernatants from mouse primary hepatocytes/293 cells transfected with EMMPRIN wild-type plasmids (WT) and EMMPRIN SNO site (cysteine 87) mutation plasmids (MUT) were collected and added to JS-1/LX2 cell medium. The MUT EMMPRIN diminishes SNO successfully, enhances the activities of MMP2 and MMP9, and subsequently increases HSC migration. In conclusion, SNO of EMMPRIN influences HSC migration and MMP activities in liver fibrosis. This finding may shed light on the possible regulatory mechanism of MMPs in ECM accumulation in liver fibrosis.

Objective To explore how to integrate the history of Peking Union Medical College (PUMC) into the teaching of diabetes in internal medicine, in order to develop an ideological and political theory-based course with PUMC characteristics. Methods The students in '4+4' medical doctor program of PUMC were enrolled into this study. Diabetes mellitus course combined with ideological and political theories course (including the history of insulin development, the life and contribution of Prof. WU Xian and Prof. LIN Kesheng, and the contributions of Prof. CHI Zhisheng, Prof. PAN Xiaoren and Prof. XIANG Hongding to the prevention and treatment of diabetes) was used for the teaching. Feedback (including the level of the political teaching knowledge before and after the course, the effectiveness of the political teaching by students after the course) was collected by questionnaire online, and statistical analysis was conducted. Results A total of 62 students (32 in grade 2020 and 30 in grade 2021) were included in the '4+4' pilot class of clinical medicine at PUMC. A total of 58 questionnaires were collected (30 in grade 2020 and 28 in grade 2021), with a rate of 93.5%(58/62). Before teaching, 55.2%~100% of the students had no knowledge of the course, and after teaching, all students had a general understanding, with the level of understanding significantly better than before (P < 0.001). According to the survey, 82.8% of the students thought that the course increased their interest in learning, 86.2% of the students were satisfied with the overall effect of the course, 89.7% thought that it allowed them to better understand the history of PUMC, 77.6% thought it deepened their understanding of the spirit of medical science, 82.8% thought it increased their understanding of the spirit of professional service to the country and patriotism, and 72.4% thought it increased their sense of social responsibility. Conclusions Integrating the history of PUMC into clinical medicine education is the unique advantage of PUMC medical education. It is conducive to students understanding of ideological and political theories, inculcation of correct values, method ology and humanistic qualities, and cultivation of the truth-seeking and pragmatic spirit of medical science.

Objective To evaluate the performance of a new fluorescence immunochromatographic assay for interleukin-6 (IL-6) detection. Methods A total of 104 serum samples from patients with suspected infections and normal population were collected at Peking Union Medical College Hospital in December, 2021. Assay A (Wanfu biofluorescence immunochromatography assay) and B (Siemens chemiluminescence assay) were used as references to evaluate the equivalence of the Innova assay for detecting serum IL-6. Meanwhile, 39 homologous paired plasma and serum were collected to evaluate the consistency of the Innova assay in detecting IL-6 content in different types of samples. Results In quantitative analysis, two samples were excluded because the content was above the limit of detection for method B. Compared with the assay A or B, the regression equations of the Innova assay were Y=-7.0950+1.1924X (R2=0.9448), Y=-2.6143+1.3072X (R2=0.9391), and the Pearson correlation coefficients were 0.9720 and 0.9691, respectively. With assay A or B as reference, the Innova assay showed the Bland-Altman bias values of -3.0 and 8.0, while the expected bias at the medical decision level (7 ng/L) was -1.44 ng/L (95% CI: -5.37 to 2.50), 1.97 ng/L (95% CI: -2.09 to 6.01), respectively, with 95% CI intervals all included permissible errors (±15%, -1.05 to 1.05 ng/L). The qualitative results showed the high total coincidence rate between the Innova assay and assay A (84.6%), and assay B (83.7%). In addition, the consistency of the Innova assay was good for 39 homologous pairs of plasma and serum samples (P < 0.0001). Only one data (2.6%, 1/39) was identified by Bland-Altman bias analysis to be outside the maximum allowable error range. Conclusions Compared with the two reference assays with the same or different principles used in clinical practice, the Innova assay for IL-6 detection has good consistency and correlation, and its test results are almost not affected by the type of serum/plasma samples. To a certain extent, the Innova assay meets the needs of rapid detection in clinical practice.

Abstract Background To study the role of microecology and metabolism in iatrogenic tracheal injury and cicatricial stenosis, we investigated the tracheal microbiome and metabolome in patients with tracheal stenosis after endotracheal intubation. Methods We collected 16 protected specimen brush (PSB) and 8 broncho-alveolar lavage (BAL) samples from 8 iatrogenic subglottic tracheal stenosis patients, including 8 PSB samples from tracheal scar sites, 8 PSB samples from scar-free sites and 8 BAL samples, by lavaging the subsegmental bronchi of the right-middle lobe. Metagenomic sequencing was performed to characterize the microbiome profiling of 16 PSB and 8 BAL samples. Untargeted metabolomics was performed in 6 PSB samples (3 from tracheal scar PSB and 3 from tracheal scar-free PSB) using high-performance liquid chromatography‒mass spectrometry (LC‒MS). Results At the species level, the top four bacterial species were Neisseria subflava, Streptococcus oralis, Capnocytophaga gingivals, and Haemophilus aegyptius. The alpha and beta diversity among tracheal scar PSB, scar-free PSB and BAL samples were compared, and no significant differences were found. Untargeted metabolomics was performed in 6 PSB samples using LC‒MS, and only one statistically significant metabolite, carnitine, was identified. Pathway enrichment analysis of carnitine revealed significant enrichment in fatty acid oxidation. Conclusion Our study found that carnitine levels in tracheal scar tissue were significantly lower than those in scar-free tissue, which might be a new target for the prevention and treatment of iatrogenic tracheal stenosis in the future.

USRP (ultrasonic surface rolling processing) was used to change the surface layer of 6061 aluminum alloy, so as to realize the change of the second phase microstructure of 6061 aluminum alloy by USRP under different static pressure conditions to improve corrosion resistant, which was characterized by using scanning electron microscopy, laser confocal microscopy, scanning Kelvin probe force microscopy, etc. Based on the principle of microzone galvanic corrosion and considering the impedance of solution and oxide, the correlation law between the size of the second phase and the development of local corrosion was obtained. The effect of the size of the second phase on the development of local corrosion was verified by in-situ observation using laser confocal microscope. The results show that the initial self-corrosion current density of aluminum alloy in 3.5%(mass fraction) NaCl solution is only 1/15 of that of the untreated sample, the corrosion rate is reduced by 93.04%, when the surface layer is rolled with 0.10 MPa static pressure. The Mg2Si phase has no significant morphology change during the rolling process, and it has no significant effect on the corrosion performance before and after USRP. The AlFeSi phases with long strip continuous distribution are refined into micro- and nano-scale dispersion distributions under the action of USRP. The fully refined AlFeSi phase weakens the galvanic corrosion effect due to the reduction of the local anode/cathode area ratio of the corrosion microcell, which promotes the metastable pitting nucleation rate of the aluminum alloy matrix, but also cause the rapid dissolution of the aluminum alloy itself. When self-dissolution occurs or Al2O3 oxide film is formed in the inner wall of metastable corrosion hole, the electrochemical corrosion effect of AlFeSi relative to aluminum alloy matrix is greatly weakened, so as to improve the corrosion resistance of 6061 aluminum alloy as a whole.

Implant infection is one of the most common and serious complications in orthopedics, and it is also an important reason for the failure of implant surgery. When bacteria form a biofilm on the implant surface, it is extremely difficult to be eliminated and attracts more bacteria and fungi. A large number of studies have shown that the use of surface modification technology can effectively reduce the adhesion and accumulation of pathogenic bacteria, thereby preventing peri-implant infection. The formation process of bacterial biofilm on the surface of orthopedic implants and the antibacterial mechanism of metal antibacterial agents were first analyzed. Then, some of the most widely used metal-based inorganic antibacterial coatings at home and abroad and their related preparation processes were reviewed, the problems and improvement methods in the application of these coatings were also discussed, and the development direction of inorganic antibacterial coatings in the future was prospected, such as synergistic antibacterial coatings and bone-promoting antibacterial coatings.

This study aimed to evaluate the multi-phase CT findings of central and peripheral pulmonary sclerosing pneumocytomas (PSPs) and compared them with Ki-67 to reveal their neoplastic nature.

Background: Little is known about the association between a plant-based diet and the risk of gallstone disease (GD), especially in developing counties. We tested the hypothesis that shifting dietary patterns would be related to the risk of GD, and that the Mediterranean diet (MED) adjusted for China would be beneficial for lowering risk of GD. Methods: Data were extracted from the baseline survey of the China Multi-Ethnic Cohort study. An alternative Mediterranean diet (aMED) score was assessed based on a food frequency questionnaire, and three posteriori dietary patterns (the modern dietary pattern, the coarse grain dietary pattern, and the rice dietary pattern) were identified using factor analysis. Multivariable logistic regression models were developed to evaluate the association between dietary patterns and GD risks. Results: A total of 89,544 participants were included. The prevalence of GD was 7.5%. Comparing the highest with lowest quintiles, aMED was associated with an increased risk of GD (OR 1.13; 95% CI, 1.04–1.24; Ptrend = 0.003), whereas the rice dietary pattern was inversely related to GD risk (OR 0.79; 95% CI, 0.71–0.87; Ptrend < 0.001). In stratified analysis, the rice dietary pattern had a stronger inverse association in the subgroups of females, older, urban, and overweight participants, and those with diabetes—factors associated with higher rates of GD in previous studies. Conclusion: Higher adherence to the rice dietary pattern was associated with a lower risk of GD. For high-risk populations, making some shift to a traditional agricultural diet might help with primary prevention of GD.

Abstract Background Somatic mutations of cancer driver genes are found to be responsible for vascular malformations with clinical manifestations ranging from cutaneous birthmarks to life-threatening systemic anomalies. Till now, only a limited number of cases and mutations were reported in Chinese population. The purpose of this study was to describe the somatic mutation spectrum of a cohort of Chinese pediatrics with vascular malformations. Methods Pediatrics diagnosed with various vascular malformations were collected between May 2019 and October 2020 from Beijing Children’s Hospital. Genomic DNA of skin lesion of each patient was extracted and sequenced by whole-exome sequencing to identify pathogenic somatic mutations. Mutations with variant allele frequency less than 5% were validated by ultra-deep sequencing. Results A total of 67 pediatrics (33 males, 34 females, age range: 0.1–14.8 years) were analyzed. Exome sequencing identified somatic mutations of corresponding genes in 53 patients, yielding a molecular diagnosis rate of 79.1%. Among 29 PIK3CA mutations, 17 were well-known hotspot p.E542K, p.E545K and p.H1047R/L. Non-hotspot mutations were prevalent in patients with PIK3CA-related overgrowth spectrum, accounting for 50.0% (11/22) of detected mutations. The hotspot GNAQ p.R183Q and TEK p.L914F mutations were responsible for the majority of port-wine stain/Sturge–Weber syndrome and venous malformation, respectively. In addition, we identified a novel AKT1 p.Q79K mutation in Proteus syndrome and MAP3K3 p.E387D mutation in verrucous venous malformation. Conclusions The somatic mutation spectrum of vascular malformations in Chinese population is similar to that reported in other populations, but non-hotspot PIK3CA mutations may also be prevalent. Molecular diagnosis may help the clinical diagnosis, treatment and management of these pediatric patients with vascular malformations.

Objective To investigate the demands of clinical teaching and perception of the theoretical knowledge and clinical practice among nursing undergraduates at school, and provide reference for establishment of a systematic teaching management system and improve the quality of clinical teaching. Methods Totally 161nursing undergraduates at school were recruited by a convenience sampling method, and an open-ended questionnaire survey was conducted to investigate the nursing undergraduates’ demands of clinical teaching. Results The top-ranked demand for clinical teaching was the combination of theoretic knowledge and clinical practice (91. 93%), followed by the diversified teaching methods including video-assisted and real case teaching (85. 71%) and developing students' interest in clinical work(81. 99%). Most of students were satisfied with clinical teaching. Conclusion Clinical teachers should help students build clinical thinking ability by adopting the teaching method combining theory with practice. Clinical teachers should help students build conference and establish identity and self-worth in clinical practice. (目的 调查在校本科护理学生对临床带教老师的需求, 通过了解学生的理论知识与临床实践认知, 为建立系统、完善的教学管理体系, 不断探索完善临床护理实践与理论知识的紧密结合, 从而为提高临床护理教学质量提供参考。方法 采用便利抽样法对某高校161例在校本科护理学生进行临床教学需求问卷调查。结果 在校生护生对“应将书本理论与临床实操练习相结合”的需求最高(91. 93%), 其次为“教学多样化, 如视频、真实案例等教学”(85. 71%)和“教学过程中应培养学生对临床工作的兴趣”(81. 99%)。满意度调查显示, 156(96. 89%)例在校护生对教学满意。结论 在进行临床教学时, 教师可以通过现场理论与实操教学相结合的方式, 让学生建立自己的临床思维, 老师多分享临床经验, 多鼓励学生, 在潜移默化中帮助学生建立自信心, 帮助护理实习生在临床工作中找到自我价值, 从而提高护理教学质量。)

The upper and middle reaches of the Yellow River is an important region in China characterized by ongoing conflicts regarding water resources, food, and energy. To achieve high-quality development of the region, it is essential to identify water, food, and energy security risks and propose corresponding measures. This study examines the basic implications of water-food-energy coordinated development and analyzes the new situation, opportunities, and challenges associated with the coordinated development of water, food, and energy in the region. Focusing on the goals of ecological protection and high-quality development in the Yellow River Basin, the study proposes a strategic framework for coordinating water, food, and energy development. It further proposes the following suggestions: (1) maximizing the utilization of water resources, (2) taking the energy industry as a pillar industry of the region and enhancing energy production efficiency, (3) ensuring food security by strengthening water support, (4) minimizing the negative impacts of food and energy development on water resources and ecology, and (4) innovating technological and institutional approaches to guarantee water, food, and energy security.

The KCl/PHPA waste drilling fluid is produced during drilling operations in Bohai Oilfield, China, which cannot be directly used or discharged, it needs to be flocculated. In order to know the aggregation mechanism of colloidal particles in KCl/PHPA drilling fluid, the calculation method of zeta potential and the relationship between the stability of waste fluid and zeta potential are discussed in detail in this article. The process of decreasing the zeta potential of waste fluid due to the addition of cationic polyacrylamide is called instability. The process in which unstable particles aggregate into larger particles is called coagulation, and the process in which unstable particles aggregate into large flocs is called flocculation. According to the types of treatment agents in KCl/PHPA waste drilling fluid, the structure and energy changes formed between low-viscosity polyanionic cellulose, partially hydrolyzed polyacrylamide, xanthan gum, starch, and water molecules are constructed. The energy of the four treatment agent systems decreased by 21.4–67.5% after the cationic polyacrylamide was added. The reduction of system energy reduced the repulsive force between colloidal particles in the waste fluid and promoted the agglomeration of colloidal particles. The agglomeration mechanism of waste liquid particles was obtained.

Owing to the feature of ultrahigh power density, the dielectric capacitors play an increasingly important role in the field of industrial production, basic scientific research, aerospace, and military industry in recent years. However, the relatively low energy storage density of the dielectric capacitors generally leads to their big sizes, which is difficult to meet the miniaturization requirements of future devices. Polymer-ceramic nanocomposites can combine high permittivity of the ceramic fillers and the excellent breakdown strength of the polymer matrix, thus achieving excellent energy storage performance. At present, developing the polymer-ceramic nanocomposites with high energy storage density is the key to realize the miniaturization goal of dielectric capacitors in the future. The current research progress of polymer-ceramic nanocomposites for energy storage capacitor applications from three perspectives was systematically summarized, including regulation of the nanofillers, optimization of the interfaces, and design of the multilayer composite structure. Notably, the influences of the dimension, size, species, hierarchical structure of the nanofillers, interface optimization methods such as surface modification and core-shell structure construction, as well as multilayer structure design such as sandwich structure and gradient structure on the permittivity, breakdown strength and the energy storage density of the nanocomposites were introduced in detail. Meanwhile, the structure-activity relationships between the microstructure of nanocomposites and their energy storage properties were further analyzed and discussed. Finally, based on the challenges and shortcomings of the current research, the important development directions of this field in the future were proposed, including selecting the new 2D nanofillers, enhancing the energy storage efficiency, employing multimode combined optimization strategy, and constructing the corresponding dielectric capacitors.

SiC fiber-bonded ceramics (FBCs), as a new thermal-structural material obtained by hot-pressing sintering of SiC fiber, has low porosity, high fiber volume fraction, high temperature resistance, oxidation resistance and high strength. It is an excellent candidate material for high temperature components such as turbine blades and rotors of aero-engine. The research progresses of FBCs were systematically reviewed in this paper. The structural characteristics and preparation methods of two types of FBCs (Tyranohex and SA Tyranohex) were introduced, the relationships among the composition, structure and performance of FBCs were discussed. It was noted that the interface carbon layer plays a crucial role in the performance of FBCs. The mechanical properties of SA-Tyranohex at room temperature and high temperature were highlighted, and the reasons for its excellent high temperature performance were analysed. The examination, verification and application development were summarized. The difficulties of FBCs in forming complex components were pointed out, and the future development trends and research directions were prospected. Finally, it was proposed that conducting research on SiC fiber-bonded ceramics is of great significance for the development of new materials for high-temperature resistant components in China.

Abstract Objective The aim of this study was to describe the time series of broad-spectrum antibiotic utilisation and incidence of antibiotic-resistant organisms during the implementation of antimicrobial stewardship programmes (ASP) in Singapore. Methods An observational study was conducted using data from 2011 to 2020 in seven acute-care public hospitals. We applied joinpoint regressions to investigate changes in antibiotic utilisation rate and incidence density of antibiotic-resistant organisms. Results Across the seven hospitals, quarterly broad-spectrum antibiotic utilisation rate remained stable. Half-yearly incidence density of antibiotic-resistant organisms with two joinpoints at first half (H1) of 2012 and second half (H2) of 2014 decreased significantly in the second and third period with a half-yearly percentage change (HPC) of -2.9% and − 0.5%, respectively. Across the five hospitals with complete data, half-yearly broad-spectrum antibiotic utilisation rate with one joinpoint decreased significantly from H1 of 2011 to H2 of 2018 (HPC − 4.0%) and H2 of 2018 to H2 2020 (HPC − 0.5%). Incidence density of antibiotic-resistant organisms decreased significantly in the two joinpoint periods from H1 of 2012 to H2 of 2014 (HPC − 2.7%) and H2 of 2014 to H2 of 2020 (HPC − 1.0%). Ceftriaxone with one joinpoint decreased significantly from H1 of 2011 to H1 of 2014 (HPC − 6.0%) and H1 of 2014 to H2 of 2020 (HPC − 1.8%) and ceftriaxone-resistant E. coli and K. pneumoniae decreased significantly in later periods, from H2 of 2016 to H2 of 2020 (HPC − 2.5%) and H1 of 2012 to H2 of 2015 (HPC − 4.6%) respectively. Anti-pseudomonal antibiotics with one joinpoint decreased significantly from H1 of 2011 to H2 of 2014 (HPC − 4.5%) and H2 of 2014 to H2 of 2020 (HPC − 0.8%) and that of quinolones with one joinpoint at H1 of 2015 decreased significantly in the first period. C. difficile with one joinpoint increased significantly from H1 of 2011 to H1 of 2015 (HPC 3.9%) and decreased significantly from H1 of 2015 to H2 of 2020 (HPC − 4.9%). Conclusions In the five hospitals with complete data, decrease in broad-spectrum antibiotic utilisation rate was followed by decrease in incidence density of antibiotic-resistant organisms. ASP should continue to be nationally funded as a key measure to combat antimicrobial resistance in acute care hospitals.

Abstract Background Genomic three-dimensional (3D) spatial organization plays a key role in shaping gene expression and associated chromatin modification, and it is highly sensitive to environmental stress conditions. In microalgae, exposure to nitrogen stress can drive lipid accumulation, yet the associated functional alterations in the spatial organization of the microalgal genome have yet to be effectively characterized. Results Accordingly, the present study employed RNA-seq, Hi-C, and ChIP-seq approaches to explore the relationship between 3D chromosomal architecture and gene expression during lipid accumulation in the marine microalga Nannochloropsis oceanica in response to nitrogen deprivation (ND). These analyses revealed that ND resulted in various changes in chromosomal organization, including A/B compartment transitions, topologically associating domain (TAD) shifts, and the disruption of short-range interactions. Significantly higher levels of gene expression were evident in A compartments and TAD boundary regions relative to B compartments and TAD interior regions, consistent with observed histone modification enrichment in these areas. ND-induced differentially expressed genes (DEGs) were notably enriched in altered TAD-associated regions and regions exhibiting differential genomic contact. These DEGs were subjected to Gene Ontology (GO) term analyses that indicated they were enriched in the ‘fatty acid metabolism’, ‘response to stress’, ‘carbon fixation’ and ‘photosynthesis’ functional categories, in line with the ND treatment conditions used to conduct this study. These data indicate that Nannochloropsis cells exhibit a clear association between chromatin organization and transcriptional activity under nitrogen stress conditions. Pronounced and extensive histone modifications were evident in response to ND. Observed changes in chromatin architecture were linked to shifts in histone modifications and gene expression. Conclusions Overall, the reprogramming of many lipid metabolism-associated genes was evident under nitrogen stress conditions with respect to both histone modifications and chromosomal organization. Together these results revealed that higher-order chromatin architecture represents a new layer that can guide efforts to understand the transcriptional regulation of lipid metabolism in nitrogen-deprived microalgae.

Abstract Recent studies raised concerns about the increasing use of gabapentinoids in different countries. With their potential for misuse and addiction, understanding the global consumption of gabapentinoids will offer us a platform to examine the need for any interventional policies. This longitudinal trend study utilised pharmaceutical sales data from 65 countries and regions across the world to evaluate the global trends in gabapentinoid consumption between 2008-2018. The multinational average annual percentage change of gabapentinoid consumption was +17.20%, increased from 4.17 defined daily dose per ten thousand inhabitants per day (DDD/TID) in 2008 to 18.26 DDD/TID in 2018. High-income countries had the highest pooled gabapentinoid consumption rate (39.92 DDD/TID) in 2018, which was more than six times higher than the lower-middle income countries (6.11 DDD/TID). The study shows that despite differences in healthcare system and culture, a consistent increase in gabapentinoid consumption is observed worldwide, with high-income countries remaining the largest consumers.

Objective By summarizing the clinical characteristics and follow-up outcomes of 5 patients with immune checkpoint inhibitor induced diabetes mellitus (ICI-DM) and reviewing the relevant literatures, the article aims at providing reference to clinicians in the diagnosis and treatment of the ICI-DM. Methods Clinical data of 5 patients with ICI-DM who were admitted to Peking Union Medical College Hospital from December 2018 to February 2023 and did retrospectively analyzed. Results Five patients with a mean age of (65±7)years received treatment by the programmed cell death 1 (PD-1) or its ligand inhibitor (PD-L1). The median time from the first immunotherapy to the discovery of elevated plasma glucose was 100 (43, 210)days, and the median cycle of immunotherapy was 7 (2.5, 10.5). The onset of the illness of all the 5 patients started with diabetic ketosis or ketoacidosis. At the onset, urine ketone bodies were positive, random plasma glucose was (36.36±15.89)mmol/L, glycosylated hemoglobin A1c (HbA1c)was (8.6%±0.66%), arterial blood pH was (7.28±0.16), and the median fasting C-peptide level was 0.09 (0.05, 0.32)μg/L. Five patients had an onset plasma glucose level of grade 3 or 4.Then, ICI treatment was discontinued in all patients and insulin therapy started. The daily dosage of insulin was (56±20)IU, supplemented with hypoglycemic drugs. After treatment, urine ketone body turned negative, pH value increased to normal range, and random plasma glucose decreased significantly (the median difference of random blood glucose before and after treatment was 21.30 mmol/L, P=0.043) showing that the treatment was effective. During the follow-up, all patients continued to use insulin. The PD-1 or PD-L1 inhibitors were restarted after hyperglycemia remission. The tumor condition was under control. Conclusions ICI-DM mainly occurs in patients who receive treatment with PD-1 or PD-L1 inhibitors usually with acute hyperglycemia whose laboratory tests indicate insulin secretion defects. Some patients had positive islet cell antibodies, glutamic acid decarboxylase antibodies and autoantibodies.Patients with positive autoantibodies needed early diagnosis and continuous insulin treatment. ICI treatment can be restarted after endocrinologists brought the blood glucose under control.

The 4+4 Medical Doctor Program set up by Peking Union Medical College has the characteristics of diverse professional backgrounds and short learning experience of medical courses. It is a pilot project to set up an integrated course of “organ-system-function”(OSF) basic medicine in the program. Teachers try to build an autonomous learning environment through the modular object-oriented dynamic learning environment(Moodle) platform in endocrine system teaching, carry out collective lesson preparation and trial lecture system, combine with the history of medicine and the introduction to medical scientific research, in order to explore a teaching model suitable for 4+4 Medical Doctor Program. According to the questionnaire survey, 80.0% of the students are generally satisfied with the teaching effect, 45.0% suggest that the basic knowledge content should be strengthened, and 55.0% of them believe that it is necessary to increase the humanistic knowledge related to the professional content.

Geomorphic features contribute to variations in regional climate, soil, and other ecological environment factors, which are also an important basis for the biodiversity and evolution of ecosystems. Studying geomorphic features in nature reserves provides a geomorphic basis for resource and environmental management and protecting ecology and biodiversity in these areas. This study revealed the terrain variation, geomorphic patterns, and developmental evolution of the Wumeng Mountain National Nature Reserve by combining quantitative and qualitative methods by investigating geomorphic features and advanced land observing satellite (ALOS) digital elevation model (DEM) terrain analysis. The results indicated that: (1) the reserve elevation was 980~2,454.7 m, with an average of 1,859.9 m, and the undulation was 390~2,104.7 m, with an average of 1,392.1 m. The basic geomorphic types of the reserve were mainly large undulating middle mountains (57.45), large undulating subalpine mountains (30.66%), and medium undulating mesas (11.17%), with an average slope of 21.1°. The gentle slopes (34.83%) had the highest proportion, followed by slopes (31.21%) and gently steep slopes (17.57%). The area of each aspect was the highest on half shady slopes (27.92%) and the least on sunny slopes (21.70%); (2) the reserve was located at the top part of the mountain, with the slope and undulation in the middle of each area being low and the surrounding area being high. From Sanjiangkou to Chaotianma and Haiziping, the average values of geomorphic parameters, such as elevation, undulation, slope, and hypsometric integral, decreased gradually, and there was a trend of progressive reduction in planation surfaces; (3) the terrain profile indicated seven planation surface levels around the reserve. Among them, levels Ⅰ-Ⅲ were the summit surfaces and residual planation surfaces, level Ⅶ was the Jinsha River erosional terrace surface, and levels Ⅳ-VI were the planation surfaces, corresponding to the three tectonic cycles in the area; (4) the hypsometric integral of the reserve ranged from 0.197 to 0.815, with an average of 0.503, and more than 80% of the area was in adulthood of geomorphic evolution, indicating that the surface erosion dynamics of the area was strong, and there was a greater ecological risk with enhanced human activities. Consequently, future conservation planning should consider differences in the surface dynamics of areas with various basic geomorphic types. Geomorphic features form an important part of the natural ecosystems of forest reserves. The unique and rich geomorphic structure enables the conservation and development of biodiversity along with the elements of the ecological environment on which it depends. Geomorphic diversity supports the analysis of the biological formation, evolution, and persistence mechanisms in reserves and provides geomorphic evidence for studying neotectonic activity in the area.

In this experiment, the peptides were obtained by enzymatic digestion of wheat germ proteins from river sets using neutral protease, trypsin and pepsin. In vitro antioxidant capacity of samples was determined and their polypeptides distribution was determined using polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that germ protein and polypeptides concentrations were positively correlated with antioxidant capacity.The antioxidant capacity of the peptides obtained by enzymatic digestion of neutral protease at different concentrations was significantly higher than that obtained by enzymatic digestion of pepsin and trypsin (P

For the analysis of the coupling characteristics of the power system frequency dynamic and power angle oscillation, the mechanism of the two-machine system under disturbances is derived in this paper, and the mechanism of the multi-machine system is obtained based on the extended equal area criterion (EEAC). A quantitative assessment index is proposed based on the Pearson coefficient, which quantified the coupling according to the characteristics of frequency dynamics and power angle oscillation. The influence of power angle oscillation on the frequency dynamic indices is analyzed, and the coupling degrees of between the power angle oscillation and different frequency dynamic indices are assessed quantitatively. The results of the mechanism analysis and case study verify the proposed quantitative indices and show the coupling characteristics of the power system frequency dynamic and power angle oscillation, which provided guidance for optimal frequency and power angle control.

Abstract Emerging photonic information processing systems require chip-level integration of controllable nanoscale light sources at telecommunication wavelengths. Currently, substantial challenges remain in the dynamic control of the sources, the low-loss integration into a photonic environment, and in the site-selective placement at desired positions on a chip. Here, we overcome these challenges using heterogeneous integration of electroluminescent (EL), semiconducting carbon nanotubes (sCNTs) into hybrid two dimensional – three dimensional (2D-3D) photonic circuits. We demonstrate enhanced spectral line shaping of the EL sCNT emission. By back-gating the sCNT-nanoemitter we achieve full electrical dynamic control of the EL sCNT emission with high on-off ratio and strong enhancement in the telecommunication band. Using nanographene as a low-loss material to electrically contact sCNT emitters directly within a photonic crystal cavity enables highly efficient EL coupling without compromising the optical quality of the cavity. Our versatile approach paves the way for controllable integrated photonic circuits.

Abstract Background The diagnosis of periprosthetic joint infection (PJI) remains a challenge in clinical practice. Many novel serum and joint fluid biomarkers have important implications for the diagnosis of PJI. The presented study evaluated the value of joint fluid interleukin-6 (IL-6) combined with the neutral polymorphonuclear leukocyte (PMN%) ratio for chronic PJI diagnosis after arthroplasty. Materials and methods Sixty patients with chronic PJI or aseptic failure who underwent hip or knee revision from January 2018 to January 2020 in our department were included in this retrospective study. According to the 2013 MSIS diagnostic criteria, the 60 patients were divided into a PJI group and a non-PJI group (30 patients per group). We collected the joint fluid before surgery and determined the level of IL-6 and the PMN% by ELISA, and the differences between the two groups were compared. The diagnostic efficacy of joint fluid IL-6 combined with PMN% in chronic PJI was analyzed using a receiver operating characteristic curve (ROC curve). Results The diagnosis of PJI using joint fluid IL-6 combined with PMN% presented an area under the curve of 0.983, which was more accurate than the areas under the curve for diagnosis using IL-6 and PMN% individually (0.901 and 0.914, respectively). The optimal threshold values for IL-6 and PMN% were 662.50 pg/ml and 51.09%, respectively. Their sensitivity and specificity were 96.67% and 93.33%, respectively. The accuracy of the diagnosis of PJI was 95.00%. Conclusions Joint fluid IL-6 combined with PMN% can be used as an auxiliary method to detect chronic infection around the prosthesis after hip/knee arthroplasty. Level of evidence Patients who underwent hip/knee revision at the First Hospital of Chongqing Medical University for periprosthetic infection or aseptic failure of the prosthesis after hip/knee arthroplasty from January 2018 to January 2020 were included. Trial registration This study was approved by the ethics committee of the First Hospital of Chongqing Medical University on September 26, 2018 (local ethics committee number: 20187101) and registered with the China Clinical Trials Registry (registration number: ChiCTR1800020440) with an approval date of December 29, 2018.

The eukaryotic microalga Nannochloropsis oceanica represents a promising bioresource for the production of biofuels and pharmaceuticals. Urea, a crucial nutrient for the photosynthetic N. oceanica, stimulates the accumulation of substances such as lipids, which influence growth and physiology. However, the specific mechanisms by which N. oceanica responds and adapts to urea addition remain unknown. High-throughput mRNA sequencing and differential gene expression analysis under control and urea-added conditions revealed significant metabolic changes. This involved the differential expression of 2104 genes, with 1354 being upregulated and 750 downregulated, resulting in the reprogramming of crucial pathways such as carbon and nitrogen metabolism, photosynthesis, and lipid metabolism. The results specifically showed that genes associated with photosynthesis in N. oceanica were significantly downregulated, particularly those related to light-harvesting proteins. Interestingly, urea absorption and transport may depend not only on specialized transport channels such as urease but also on alternative transport channels such as the ABC transporter family and the CLC protein family. In addition, urea caused specific changes in carbon and lipid metabolism. Genes associated with the Calvin cycle and carbon concentration mechanisms were significantly upregulated. In lipid metabolism, the expression of genes associated with lipases and polyunsaturated fatty acid synthesis was highly activated. Furthermore, the expression of several genes involved in the tricarboxylic acid cycle and folate metabolism was enhanced, making important contributions to energy supply and the synthesis and modification of genes and macromolecules. Our observations indicate that N. oceanica actively and dynamically regulates the redistribution of carbon and nitrogen after urea addition, providing references for further research on the effects of urea on N. oceanica.

The repairing and strengthening of concrete structures using external and internal partial confinements are inevitable in the construction industry due to the new standards and rapid developments. The conventional materials and methods of confinement are unable to meet modern safety and functional standards. The fiber-reinforced polymer (FRP) enhances the strength and ductility of deteriorating and new concrete columns by reducing lateral confinement pressure and resistance against seismic shocks. The precise methods of partial confinement are inevitable for effective FRP-concrete bonding, durability, and cost-effectiveness under different loading conditions and to cope with external environmental factors. Predictive modeling and simulation techniques are pivotal for the optimization of confinement materials and methods by investigating the FRP-concrete novel confinement configurations, stress–strain responses, and failure modes. The novel materials and methods for concrete columns’ partial confinement lack high compressive strength, ductility, chemical attack resistivity, and different fiber orientation impacts. This review provides an overview of recent confinement materials, novel methods, and advanced modeling and simulation techniques with a critical analysis of the research gaps for partial FRP confinement of concrete columns. The current challenges and future prospects are also presented.

Aiming at effectively generating safe and reliable motion paths for quadruped robots, a hierarchical path planning approach driven by dynamic 3D point clouds is proposed in this article. The developed path planning model is essentially constituted of two layers: a global path planning layer, and a local path planning layer. At the global path planning layer, a new method is proposed for calculating the terrain potential field based on point cloud height segmentation. Variable step size is employed to improve the path smoothness. At the local path planning layer, a real-time prediction method for potential collision areas and a strategy for temporary target point selection are developed. Quadruped robot experiments were carried out in an outdoor complex environment. The experimental results verified that, for global path planning, the smoothness of the path is improved and the complexity of the passing ground is reduced. The effective step size is increased by a maximum of 13.4 times, and the number of iterations is decreased by up to 1/6, compared with the traditional fixed step size planning algorithm. For local path planning, the path length is shortened by 20%, and more efficient dynamic obstacle avoidance and more stable velocity planning are achieved by using the improved dynamic window approach (DWA).

Anomalous bright spots, called the string of bead-like response, are typical seismic migration profile features in carbonate fractured reservoirs in the Tarim Basin, and they are indicators of high-quality oil and gas reservoirs. Correctly recognizing the correspondence between fractures and the SBLR can contribute to the efficient drilling of target carbonate fractured reservoirs. Physical models can describe fractured reservoirs more directly and efficiently than real situations and have obvious advantages in accurately and quantitatively designing parameters such as dipping angles and the number of layers of fractured reservoirs. Under such a background, according to the real tectonic characteristics of the Tarim Basin, among the main hydrocarbon reservoirs, fractured reservoirs with various parameters were designed and a physical model was constructed according to the real stratigraphic parameters. After seismic data acquisition and processing, the response characteristics of the string of bead-like response were extracted and summarized from seismic migration profiles for all fractured reservoirs, which provided targeted analyses and discussions on the fracture dipping angle, number of fracture layers, overlying stratigraphic influences, and planar attributes of the fractured reservoirs. In general, the larger the fracture dip, the more difficult it is to identify, while the slope of reflection strength and maximum absolute amplitude attributes can be important markers for fractured reservoir identification. The physical modeling study of fractured reservoirs in this paper can provide a basis for the analysis and prediction of carbonate fractured reservoirs in the Tarim Basin.

Under the influence of a variety of unfavorable factors, slope instability may occur, so the stability of the slope needs to be analyzed. In this paper, combined with the geological investigation data, the slope engineering geological profiles are derived based on 2D inversion maps of the high-density resistivity (HDR) method to obtain the overburden layer thickness. Then, a finite element model is established based on the mechanical and geometric parameters of the slope to analyze the displacement and deformation characteristics and obtain information about the potential sliding surface. The above analysis results are combined to determine the effective monitoring field and deploy (Fiber Bragg Grating) FBG strain detection piles for deep strain monitoring. The analysis showed that the shape and location of the potential sliding surface of the slope from the numerical simulation are consistent with those delineated by the HDR profile. The monitoring results show the rock and soil on the north side of the slope are soft and have poor stability, which is consistent with the HDR method detection results. Adopting the HDR method to identify the slope’s overall geological structure combined with FBG strain detection piles to obtain the deep deformation provided an effective monitoring technique for slope stability assessment.

Granular flow is one of the most destructive geological hazards in mountainous areas, posing a severe threat to the economy and personnel safety in the region. Shed tunnels are widely used for the prevention and mitigation of granular flow hazards. Thus, comprehensively studying the impact mechanisms of granular flows on shed tunnels is significant for disaster prevention and mitigation. This study adopts a combined approach of a physical model experiment and Particle Flow Code (PFC) simulation to investigate the impact force of granular flow on shed tunnels and the buffering effect of cushion layers. The influences of slope angle, cushion layer thickness, and cushion layer particle size are discussed. It is revealed that as the slope angle increases, the velocity of the granular flow and the impact force on the shed rise significantly. When the slope angle increases from 40° to 60°, the peak velocity surges by 25%, while the impact force intensifies by 2–3 times. Moreover, increasing the thickness of the cushion layer can mitigate the interaction between the granular flow and the shed tunnel, thereby enhancing structural safety. With an increase in cushion layer thickness from 0 to 200 mm, the impact force is reduced by approximately 50%. Meanwhile, reducing the particle size of the cushion layer effectively decreases the impact force, resulting in less kinetic energy and providing a stronger cushioning effect.

Seafloor instability and secondary submarine geohazards are widely present in the ocean, posing a threat to submarine infrastructures such as coastal port facilities, offshore drilling platforms, and submarine pipelines and fiber-optic cables. However, formation mechanisms and controlling factors of seafloor instability are still poorly understood. To improve the understanding, based on the history and development of seafloor instability, this study sorts out the common categories, global distribution, and geophysical characteristics, analyzes the formation mechanisms, controlling factors, and engineering geohazards risks, and summarizes popular quantitative analysis methods for seafloor instability. Subsequently, the application and limitations of experimental simulation technology for the slope instability process are discussed. Focused on the disaster mechanism, intelligent analysis of multi-source data, and three-dimensional monitoring of seabed instability, this study proposed the development direction and countermeasures of future seafloor instability research, aiming to provide guiding suggestions for the simulation, prediction, and warning of seafloor instability.

ObjectiveTo analyze the effect of O3 pollution on outpatient visits for respiratory diseases in a district of Shanghai.MethodsWe collected the respiratory disease outpatient data， and atmospheric and meteorological data of from a suburban general hospital in Shanghai from 2015 to 2017. A time-series analysis by generalized additive model was conducted to examine the relationship between O3 pollution and daily outpatient visits.ResultsThe daily outpatient volume for respiratory diseases was 831. The daily 8 h median concentration of O3 was 101.04 μg·m-3. The excess relative risk was 0.461% （95%CI： 0.240%‒0.682%） at lag3. Stratified by gender and age， females， child and the aged had higher risk of respiratory diseases. In the double-pollutant model， PM2.5 and PM10 increased health effects， while CO reduced health effects.ConclusionThe increase of O3 concentration can increase the daily outpatient volume of respiratory diseases.

Older population migration has become a normal occurrence that requires further systematic attention. The reasons for older migration in contemporary China exhibit a diversified trend. However, existing literature rarely discusses the impact of migration reasons on the settlement intention of the mobile older population. In China, where the aging demographic is increasing, the residence choice of the older floating population with their families presents a significant difference among cities and regions. Therefore, from the perspective of comparative analysis, to address the gap in the literature, there is an urgent need to reveal the influence mechanism of the settlement intention of the floating older population under different categories of reasons for mobility. Such an approach is of great significance for local governments to conduct differentiated management of the floating older population according to local conditions, provide equal health care services for older people, and promote a new wave of urbanization. In addition to continuous aging and urbanization in China, the migration reasons and characteristics of the older migrant population are diversified. While family migration is still the main reason for the mobility of older people in China, there is an increasingly evident demand for off-site pension, which presents new requirements for the government to actively respond to the aging and carry out services for different kinds of migrant older people. This study explores the influence mechanism of two typical mobility reasons (off-site pension and family migration) on the settlement intention of migrant older people. We adopt the multi-level logistic regression model based on the 2017 China Migrant Population Dynamic Survey. This study derives the following main results. First, the settlement intention of off-site older people is significantly promoted, while the intentions of the family accompany-migrated older people are not convincing. The intention of the former is 14.3% higher than the latter. Second, personal and family characteristics (demographic factors, socioeconomic status, social integration status) and inflow characteristics (urban scale, urban public services, urban environment) both affect the settlement intention of migrant older people. Third, there are different mechanisms for the settlement intention of migrant older people with different reasons for migration. The family is the core of the settlement choice for family migration, while the livability of the environment mitigates its effect. Fourth, the settlement choice of the off-site older people depends on the purpose of personal pension. This kind of migrant older people focus more on external conditions such as favorable policies of urban agglomeration and convenient pension facilities. They will be more easily affected by the social network connection of the inflow place. An in-depth understanding of the migration reasons and characteristics of migrant older people can aid in the provision of better public services. The conclusions of this study provide a reference for local governments to carry out differentiated governance of migrant older people, promote new urbanization, and equalize public services.

Abstract Background Kawasaki disease (KD) is a medium vessel vasculitis, of unknown etiology, typically presenting in children younger than 5 years of age. Prolonged fever (at least five days) is a major clinical criterion of KD, while cardiac involvement may occur in up to 25% of patients, generally in the second week of the disease. Case presentation We describe the case of KD developing in a 3-month infant, with an early occurrence of coronary artery aneurysm after only 3 days of fever, complicated by thrombosis, requiring aggressive treatments. Conclusions Time of development of cardiac complications can be different in young infants with KD and both diagnostic criteria and treatment indications should be individualized in this class of age.

Long noncoding RNAs (lncRNAs) have been widely proven to be involved in liver lipid homeostasis. Herein, we identify an upregulated lncRNA named lncRP11-675F6.3 in response to rapamycin treatment using a microarray in HepG2 cells. Knockdown of lncRP11-675F6.3 leads to a significant reduction in apolipoprotein 100 (ApoB100), microsomal triglyceride transfer protein (MTTP), ApoE and ApoC3 with increased cellular triglyceride level and autophagy. Furthermore, we find that ApoB100 is obviously colocalized with GFP-LC3 in autophagosomes when lncRP11-675F6. 3 is knocked down, indicating that elevated triglyceride accumulation likely related to autophagy induces the degradation of ApoB100 and impairs very low-density lipoprotein (VLDL) assembly. We then identify and validate that hexokinase 1 (HK1) acts as the binding protein of lncRP11-675F6.3 and mediates triglyceride regulation and cell autophagy. More importantly, we find that lncRP11-675F6.3 and HK1 attenuate high fat diet induced nonalcoholic fatty liver disease (NAFLD) by regulating VLDL-related proteins and autophagy. In conclusion, this study reveals that lncRP11-675F6.3 is potentially involved in the downstream of mTOR signaling pathway and the regulatory network of hepatic triglyceride metabolism in cooperation with its interacting protein HK1, which may provide a new target for fatty liver disorder treatment.

Charge transfer plasmon (CTP) modes arise when metallic nanoparticle dimers are connected by a conductive junction. This offers a unique opportunity to explore electron transport at optical frequencies as well as to attain narrow plasmon resonances that can be broadly modulated from visible to IR regimes, implying their potentials for applications in single-molecule electronics and sensing. This article showcases recent developments in theory and applications of charge transfer plasmon resonances (CTPRs) in various configurations of conductively linked plasmonic nanodimers. In particular, we give a due attention to exploiting ultratunable spectral features of charge transfer plasmon resonances for developing multifunctional plasmonic sensors for bulk, surface, gas and molecular sensing applications. We also discuss the implications of the charge and energy transfer between two plasmonic nanoparticles linked by sub-nanometer thick self-assembled monolayers for single-molecule conductance sensing and molecular electronics. In addition to the well-established plasmonic sensing schemes based on propagating and localized surface plasmon resonances, charge transfer plasmon resonance sensors may open up a new route in efforts to develop multifunctional sensing technologies.

Cultural memory is a spiritual, inner, and middle world composed of self-consciousness, memory, and behavior that expresses the cultural phenomenon, local emotions, and identity generated by human practice in the present world space. Geography pays more attention to the human-land relationship and spatial perception in the formation of cultural memory and constructs a unique cultural memory space. This space is the agglomeration of figurative cultural relics in space, as well as the spiritual representation of non-figurative places, such as art, text, and folklore in space. By systematically analyzing the theory of cultural memory, this study summarizes the process of evolution and development context of cultural memory. Based on 1,527 key domestic and international studies on geography, this study analyzes the main content and progress of global cultural memory space research from the perspective of keyword co-occurrence clustering, theme path evolution, and research trend, with the assistance of CiteSpace—the bibliometrics and visualization tool. By reviewing the frontier hot spots of cultural memory space research at home and abroad, this study aims to construct a research paradigm of cultural memory from the perspective of geography, improve the research methods of cultural memory, and provide a reference for the local application and multidisciplinary integration of cultural memory theory. The analysis results focus the research topics of foreign cultural memory space primarily on: 1) the memory representation of the landscape of daily life; and 2) political and ritual studies in memory. Domestic research topics primarily include: 1) cultural and local studies in collective and social memory; 2) research on urban and rural memory carried by space; and 3) research on heritage memory reproduction, promoted by cultural tourism. The literature on cultural memory space at home and abroad has gradually increased in recent years, as has the attention paid to memorial landscape, heritage buildings, ritual celebrations, war trauma, and national memory; however, the research on cultural memory space has not formed a specific theoretical paradigm. Most of the literature only focuses on the integration of cultural memory and other fields, and relatively few studies address the connotation of cultural memory space, media conversion mechanisms, and spatial identification methods. The perspective of the content and trends in domestic and foreign research indicates that Western scholars pay more attention to the study of migration, rights, free will, women, power politics, and war trauma. The focus on space is mainly on cities, emotions, and rights. Domestic geographers focus on deconstructing regionalized and China-seized cultural memory and local identity from the intangible carrier of memory. Most studies are based on obvious policy orientations. War sites, red tourist sites, traditional villages, characteristic folk customs, and festival ceremonies have become important themes in the study of cultural memory space. In the future, geography research should depict the practical process of text, ritual, and language through non-representational means and re-introduce memory into human research in a materialized way. Simultaneously, the connotation of urban and rural cultural memory should be clearly defined, and heritage, immigration, and tourism should be adopted as the new themes of cultural memory space research through digital images.

The development history, market overview and development status of viscosity index improver for hydrogenated styrene-diene copolymer at home and abroad were systematically summarized with 38 references, and the basic classification, performance characteristics and preparation methods of viscosity index improver were briefly discussed. Finally, the development direction and trend of hydrogenated styrene-diene copolymer viscosity index improver in the future were pointed out.

Abstract Background More lung cancer cases are becoming diagnosed earlier in recent years. The diagnosis is often accompanied by fear of progression (FoP). There is a clear research gap in the existing literature on FoP and the most frequent concerns in newly diagnosed lung cancer patients. Objective To identify the status and factors related to FoP in newly diagnosed Chinese lung cancer patients undergoing thoracoscopic lung cancer resection. Methods A cross-sectional design with convenience sampling was used in this study. Participants (N = 188) with newly diagnosed lung cancer (≤ 6 months) at one hospital in Zhengzhou were recruited. A demographic questionnaire, Fear of Progression Questionnaire-Short Form, Social Support Rating Scale (SSRS), Simplified Coping Style Questionnaire, and Brief Illness Perception Questionnaire were used to assess characteristics, FoP, social support, coping style, and patient illness perceptions. Multivariable logistic regression analysis was used to identify factors associated with FoP. Results The mean score of FoP was 35.39 ± 8.03. There are 56.4% of the patients (scores ≥ 34) have a clinically dysfunctional level of FoP. FoP was higher in young (18–39 years) than middle-aged patients (40–59 years) and elderly patients (≧60 years) (P = 0.004). Patients aged 40–59 years showed significantly higher fear of family-related concerns (P

Objective: To explore whether Cyclic Adenosine Monophosphate (cAMP)-Epac1 signaling is activated in 1-Desamino-8-D-arginine-Vasopressin-induced Endolymphatic Hydrops (DDAVP-induced EH) and to provide new insight for further in-depth study of DDAVP-induced EH. Methods: Eighteen healthy, red-eyed guinea pigs (36 ears) weighing 200–350 g were randomly divided into three groups: the control group, which received intraperitoneal injection of sterile saline (same volume as that in the other two groups) for 7 consecutive days; the DDAVP-7d group, which received intraperitoneal injection of 10 mg/mL/kg DDAVP for 7 consecutive days; and the DDAVP-14d group, which received intraperitoneal injection of 10 μg/mL/kg DDAVP for 14 consecutive days. After successful modeling, all animals were sacrificed, and cochlea tissues were collected to detect the mRNA and protein expression of the exchange protein directly activated by cAMP-1 and 2 (Epac1, Epac2), and Repressor Activator Protein-1 (Rap1) by Reverse Transcription (RT)-PCR and western blotting, respectively. Results: Compared to the control group, the relative mRNA expression of Epac1, Epac2, Rap1A, and Rap1B in the cochlea tissue of the DDAVP-7d group was significantly higher (p

Urban creative networks, as distinct forms of production networks, differ from inter-firm-based urban networks. It is featured by flexible production, "temporary cooperation" and being "people-centered." This study attempts to address the concept of the urban creative network in theory, and based on the basic database of Chinese digital music, it integrates the social network, Geographical Information System (GIS) spatial analysis, and econometric model to empirically analyze the structural evolution characteristics and influencing factors of the creative cooperation network of Chinese singers in the past 40 years, which enriches the theoretical and empirical study of urban networks from the perspective of creative individual connections. The results suggest the following: (1) The scale of Chinese urban digital music collaborative networks grows rapidly and shows the characteristics of low density and a "small world", and network density is negatively correlated with network scale, with obvious social network properties. (2) The collaborative network as a whole presents a "core-periphery" structure and the development trend of polycentricity and high-centricity cities are mainly located in the eastern region, with relatively low centrality in the central and western cities, and gradually forms a triad structure of Beijing-Hong Kong-Taipei. (3) The evolution of the digital music cooperation network is characterized by both path dependence and path creation, and the main form of network extension is hierarchical diffusion, with the early high-intensity links between Hong Kong and Taiwanese cities gradually being replaced by mainland cities. (4) The coverage of city ties in the cooperative network expands with an uneven spatial distribution, and high-intensity ties are mainly concentrated in the eastern cities. Over time, the core cities' control over network resources through inter-high-intensity ties weakened. (5) The cooperative network has an obvious community structure, and the communities of this coproduction network appear to be expanding, grouping, and hierarchical, with dual-core and multi-core models as its main spatial organization modes. (6) A negative binomial regression model analysis shows that the scale of the urban economy, industrial structure, and the level of the network economy have significant impacts on the network structure, while the roles of human capital, opening up, urban administrative level, and traffic accessibility are not evident, indicating that the Chinese music cooperation network has obvious endogenous mechanisms and local embeddedness. The findings of this study provide in-depth theoretical and policy insights for understanding intercity interactions and promoting the development of creative industries under the influence of the creative (digital) economy. First, a study on city networks based on the production networks of digital creative industries should focus on the characteristics of creative production networks and the role of creative actors (people). Second, policymakers should fully understand the characteristics of digital creative production sector actors and production modes when formulating policies for the development of digital creative industries and choose development paths that fit the advantages of local resources to realize industrial development. Finally, we propose a future research agenda for urban creative networks.

Abstract Sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) is indispensable in organ development because it maintains intracellular cholesterol homeostasis. The vessel is not widely conceived of as a cholesterol-sensitive tissue, so the specific role of SCAP in angiogenesis has not been paid attention to. As an important component of the vascular mesoderm, vascular smooth muscle cells (VSMCs) are widely involved in each step of angiogenesis. Here, we report for the first time that VSMC-specific ablation of SCAP inhibits VSMC proliferation and migration, interacting with endothelial cells (ECs), and finally causes defective embryonic angiogenesis in mice. Mechanistically, we demonstrated that SCAP ablation in VSMCs leads to the upregulation of KISS-1 protein, consequently resulting in suppressed activation of the MAPK/ERK signaling pathway and downregulation of matrix metalloproteinase 9 (MMP9) and vascular endothelial-derived growth factor (VEGF) expression to prevent angiogenesis. Importantly, we found that SCAP promotes the cleavage and nuclear translocation of SREBP2, which acts as a negative transcription regulator, regulating KISS-1 expression. Our findings suggest that SCAP contributes to embryonic angiogenesis by negatively regulating KISS-1 expression in mice and provide a new point of view for therapeutic targets of vascular development.