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465 results on '"Li, Jishan"'

103. Molecular Engineering of α-Substituted Acrylate Ester Template for Efficient Fluorescence Probe of Hydrogen Polysulfides

Author

Guo, Jingru, Yang, Sheng, Guo, Chongchong, Zeng, Qinghai, Qing, Zhihe, Cao, Zhong, Li, Jishan, and Yang, Ronghua

Abstract

In this article, hydrogen polysulfide (H2Sn)-mediated Michael addition/cyclization cascade reactions toward acrylate ester analogues were exploited and utilized to construct novel and robust H2Sn-specific fluorescence probe for the first time. Through rational molecular engineering of the α-substituted acrylate ester template, the optimal candidate probe FP–CF3containing trifluoromethyl-substituted acrylate ester group as recognition unit and 3-benzothiazol-7-hydroxycoumarin dye BHCas signal reporter can highly selectively detect H2Snover other reactive sulfur species, especially biothiols including cysteine (Cys) and homocysteine (Hcy)/glutathione (GSH), with a rapid and significant turn-on fluorescence response (less than 60 s for response time and over 44-fold for signal-to-background ratio). The fast response and high selectivity of FP–CF3for H2Sncould be attributed to a kinetically and spatially favored pentacyclic addition produced by the dual nucleophilic reaction of H2Snwith the CF3-substituted acrylate group. The big off–on fluorescence response is due to the pentacyclic intermediate results in the release of the highly fluorescent BHC. Moreover, it has been successfully applied in imaging of endogenous H2Snfluctuation in living cells.

Published
2024
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104. Alkynes-DNA-Functionalized Alloyed Au/Ag Nanospheres for Ratiometric SERS Imaging Assay of Endonuclease Activity in Live Cells

Author

Si, Yanmei, Bai, Yaocai, Qin, Xiaojie, Li, Jun, Zhong, Wenwan, Xiao, Zhijun, Li, Jishan, and Yin, Yadong

Abstract

A novel ratiometric surface-enhanced Raman scattering (SERS) nanosensor has been developed to probe the activity of endonuclease under in vitro and in living cells conditions. The optimized alloyed Au/Ag nanoparticles (Au/AgNPs) were synthesized as the SERS substrate, which combined the superior properties of both the pure Au and Ag nanoparticles: they exhibit excellent plasmonic property with high chemical stability and low cytotoxicity. They were then employed for quantitative detection of endonuclease through functionalization with single-stranded DNA (ssDNA) carrying 3-(4-(phenylethynyl)benzylthio) propanoic acid (PEB) as endonuclease-responsive SERS signaling molecule, and 4-thiol phenylacetylene (TPA) as the internal standard SERS signaling molecule. In the presence of endonuclease, the ssDNA was cleaved, releasing the PEB molecules from the particle surface and decreasing the SERS signal at 2215 cm-1 from PEB. Since the SERS signal at 1983 cm-1 from alkynyl TPA remained the same, quantitative detection of endonuclease was achieved based on the ratiometric peak intensity of I1983/I2215, with a detection limit as low as 0.056 U/mL. A highly biocompatible and anti-jamming ratiometric SERS sensor was established by combining the alloyed Au/AgNPs with two unique alkynes molecules with Raman signal in the cellular silent region. The ratiometric sensor was successfully employed to detect intracellular endonuclease activity as well as endonuclease in living cells for the first time.

Published
2024
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110. Membraneless reproducible MoS2field-effect transistor biosensor for high sensitive and selective detection of FGF21

Author

Gong, Xinxing, Liu, Yeru, Xiang, Haiyan, Liu, Hang, Liu, Zhigang, Zhao, Xiaorui, Li, Jishan, Li, Huimin, Hong, Guo, Hu, Travis, Chen, Hong, Liu, Song, and Yu, Gang

Abstract

Fibroblast growth factor 21 (FGF21) serves as an essential biomarker for early detection and diagnosis of nonalcoholic fatty liver disease (NAFLD). It has received a great deal of attention recently in efforts to develop an accurate and effective method for detecting low levels of FGF21 in complex biological settings. Herein, we demonstrate a label-free, simple and high-sensitive field-effect transistor (FET) biosensor for FGF21 detection in a nonaqueous environment, directly utilizing two-dimensional molybdenum disulfide (MoS2) without additional absorption layers. By immobilizing anti-FGF21 on MoS2surface, this biosensor can achieve the detection of trace FGF21 at less than 10 fg mL−1. High consistency and satisfactory reproducibility were demonstrated through multiple sets of parallel experiments for the MoS2FETs. Furthermore, the biosensor has great sensitivity to detect the target FGF21 in complex serum samples, which demonstrates its great potential application in disease diagnosis of NAFLD. Overall, this study shows that thin-layered transition-metal dichalcogenides (TMDs) can be used as a potential alternative platform for developing novel electrical biosensors with high sensitivity and selectivity. 成纤维细胞生长因子21(FGF21) 是一种用于早期检测和诊断 非酒精性脂肪肝病(NAFLD)的必需生物标志物.最近,开发在复杂 生物环境中准确有效地检测血液中低浓度FGF21的方法受到了极 大的关注.本文展示了一种无标记、简单和高灵敏度的场效应晶 体管 (FET)生物传感器,用于在非水环境中检测FGF21. 通过对二硫 化钼(MoS2)表面进行功能化将抗FGF21牢固地固定在MoS2材料上, 使该生物传感器实现检测FGF21的检测限小于10 fg mL−1.多组平 行实验证明了MoS2FET 的高度一致性和令人满意的再现性.此外, 生物传感器对复杂血清样品中的目标FGF21具有很高的敏感度,这 表明其在NAFLD疾病诊断中具有巨大的潜在应用前景.

Published
2019
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