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Additional file 5: Table S5. Codon usages of all protein coding genes in 10 assembled chloroplast genomes in subfamily Zingiberoideae.

Additional file 1: Table S1. Characteristics of 10 assembled chloroplast genomes in subfamily Zingiberoideae.

Additional file 3: Table S3. Genes with introns in 10 assembled chloroplast genomes of subfamily Zingiberoideae.

Additional file 6: Table S6. List of RNA editing sites in 10 assembled chloroplast genomes of subfamily Zingiberoideae as predicted by the PREP program [67].

Tumor metastasis, the leading cause of death among cancer patients, is a complex multi-step process during which tumor cells disseminate from their primary site to distant organs. The transcription factor Twist1 is overexpressed in many aggressive human cancers and is a potent inducer of tumor metastasis. Mechanistically, Twist1 activates a developmental program known as the Epithelial-Mesenchymal Transition (EMT), during which carcinoma cells lose cell- cell junctions and become more migratory and invasive. Twist1 can also induce the formation of invadopodia, actin -rich membrane protrusions that recruit various proteases to degrade the extracellular matrix (ECM). Various studies show that invadopodia are crucial for tumor invasion during metastasis. We have identified the membrane- associated serine protease Fibroblast Activation Protein (FAP) to be a critical gene induced upon Twist1 activation to promote invadopodia function. However, the precise role of FAP at invadopodia and its function in tumor metastasis is not well understood. This study presents data demonstrating that FAP is essential for invadopodia- mediated ECM degradation and breast tumor metastasis in vivo. Mechanistically, I found that FAP is required for the proper localization of matrix metalloproteinase MT1- MMP to invadopodia in order to generate mature and functional invadopodia for EMT degradation. Together, these results indicate that FAP is a critical regulator of invadopodia function and tumor metastasis. Given the unique expression of FAP in human cancers, FAP could be a promising target for anti-metastasis therapeutics

Oulenziella Fan & Zhang, 2015 Oulenziella Fan & Zhang in Fan et al., 2015: 192; type species: Calvolia bakeri Hughes, 1962: 59., Published as part of Fan, Qing-Hai, Li, Dong-Mei & George, Sherly, 2020, Ontogenetic stages of Oulenziella bakeri (Hughes) (Acari: Winterschmidtiidae), pp. 62-101 in Zootaxa 4900 (1) on page 63, DOI: 10.11646/zootaxa.4900.1.7, http://zenodo.org/record/4408968, {"references":["Fan, Q. - H., George, S., Li, D. & Zhang, Z. - Q. (2015) Establishment of Oulenziella gen. nov. for Oulenzia bakeri (Hughes, 1962) (Acari: Winterschmidtiidae). Zootaxa, 3949 (2), 191 - 202. https: // doi. org / 10.11646 / zootaxa. 3949.2.2","Hughes, A. M. (1962) The genus Calvolia Oudemans, 1911 (Acari: Sarcoptiformes). Acarologia, 4 (1), 48 - 63."]}

Key to life stages of Oulenziella bakeri (Hughes) 1. With 4 pairs of legs, genital opening and 1 or 2 pairs of genital papillae (Fig. 2); hysterosoma with 12 pairs of setae (f 2 present) (Figs 1 & 2); Claparède organ absent...................................................................... 2 - With 3 pairs of legs; genital opening and papillae absent (Fig. 18); hysterosoma with 11 pairs of setae (f 2 absent) (Fig. 17); Claparède organ present between coxae I and II (Fig. 18)................................................... larva 2. With 2 pairs of genital papillae (Fig. 2); ventral setae 3a and 4a present (Fig. 2); solenidion ω 3 on tarsus I present (Fig. 4A); tibia IV with a solenidion and a seta (Fig. 4D)............................................................... 4 - With 1 pair of genital papillae (Fig. 14); ventral setae 3a and 4a absent (Fig. 14); solenidion ω 3 on tarsus I absent (Fig. 16A); tibia IV nude (Fig. 16D)....................................................................... protonymph 4. Genital opening inverted V-shaped (Fig. 2); with either spermatheca (Figs 1, 3G & H) or aedeagus (Fig. 6)..... (adult). … 5 - Genital opening a simple longitudinal slit (Fig. 10); without spermatheca or aedeagus (Fig. 10)............... tritonymph 5. Aedeagus present (Fig. 6); without a spermatheca; tarsi I and II each with a ventro-terminal sucker (Fig. 8A & B)..................................................................................................... adult male - Aedeagus absent but with a spermatheca (Figs 1, 3G & H); tarsi I and II without ventro-terminal sucker (Fig. 4 A & B)............................................................................................... adult female, Published as part of Fan, Qing-Hai, Li, Dong-Mei & George, Sherly, 2020, Ontogenetic stages of Oulenziella bakeri (Hughes) (Acari: Winterschmidtiidae), pp. 62-101 in Zootaxa 4900 (1) on pages 95-96, DOI: 10.11646/zootaxa.4900.1.7, http://zenodo.org/record/4408968

Three new diterpenoids (1–3) (two abietane type diterpenoids and a paralianone type diterpenoid), together with four known compounds (4–7) were isolated from the whole plants of Euphorbia peplus. Their structures were elucidated through spectroscopic analysis and physicochemical characteristics. The cytotoxic activities of compounds 1–7 against five human tumour cell lines were evaluated, however, they were inactive at the concentration of 40 μM. The compound 3 enhanced lysosomal biogenesis with Lyso Tracker staining intensity of 132.6%.