113 results on '"Leidinger Petra"'
Search Results
102. Novel autoantigens immunogenic in COPD patients
- Author
-
Leidinger, Petra, primary, Keller, Andreas, additional, Heisel, Sabrina, additional, Ludwig, Nicole, additional, Rheinheimer, Stefanie, additional, Klein, Veronika, additional, Andres, Claudia, additional, Hamacher, Jürg, additional, Huwer, Hanno, additional, Stephan, Bernhard, additional, Stehle, Ingo, additional, Lenhof, Hans-Peter, additional, and Meese, Eckart, additional
- Published
- 2009
- Full Text
- View/download PDF
103. A Different View on DNA Amplifications Indicates Frequent, Highly Complex, and Stable Amplicons on 12q13-21 in Glioma
- Author
-
Fischer, Ulrike, primary, Keller, Andreas, additional, Leidinger, Petra, additional, Deutscher, Stephanie, additional, Heisel, Sabrina, additional, Urbschat, Steffi, additional, Lenhof, Hans-Peter, additional, and Meese, Eckart, additional
- Published
- 2008
- Full Text
- View/download PDF
104. Comprehensive analysis of microRNA profiles in multiple sclerosis including next-generation sequencing.
- Author
-
Keller, Andreas, Leidinger, Petra, Steinmeyer, Florian, Stähler, Cord, Franke, Andre, Hemmrich-Stanisak, Georg, Kappel, Andreas, Wright, Ian, Dörr, Jan, Paul, Friedemann, Diem, Ricarda, Tocariu-Krick, Beatrice, Meder, Benjamin, Backes, Christina, Meese, Eckart, and Ruprecht, Klemens
- Subjects
- *
MICRORNA , *MULTIPLE sclerosis diagnosis , *MAGNETIC resonance imaging , *TISSUE wounds , *BIOMARKERS - Abstract
The article presents a comprehensive analysis of microRNA (miRNA) profiles in multiple sclerosis (MS), including next-generation sequencing (NGS). The analysis was performed in blood of patients with clinically isolated syndrome or relapsing-remitting multiple sclerosis. An overview of the methods of the study is given, along with a discussion on the results and conclusions of the comprehensive analysis.
- Published
- 2014
- Full Text
- View/download PDF
105. A set of specific miRNAs is connected with murine and human gastric cancer.
- Author
-
Shah, Aftab Ali, Leidinger, Petra, Backes, Christina, Keller, Andreas, Karpinski, Pawel, Sasiadek, Maria M., Blin, Nikolaus, and Meese, Eckart
- Published
- 2013
- Full Text
- View/download PDF
106. High-throughput miRNA profiling of humanmelanoma blood samples.
- Author
-
Leidinger, Petra, Keller, Andreas, Borries, Anne, Reichrath, Jörg, Rass, Knuth, Jager, Sven U., Lenhof, Hans-Peter, and Meese, Eckart
- Subjects
- *
NEUROENDOCRINE tumors , *CANCER patients , *BLOOD cells , *BIOMARKERS , *MELANOMA - Abstract
Background: MicroRNA (miRNA) signatures are not only found in cancer tissue but also in blood of cancer patients. Specifically, miRNA detection in blood offers the prospect of a non-invasive analysis tool. Methods: Using a microarray based approach we screened almost 900 human miRNAs to detect miRNAs that are deregulated in their expression in blood cells of melanoma patients. We analyzed 55 blood samples, including 20 samples of healthy individuals, 24 samples of melanoma patients as test set, and 11 samples of melanoma patients as independent validation set. Results: A hypothesis test based approch detected 51 differentially regulated miRNAs, including 21 miRNAs that were downregulated in blood cells of melanoma patients and 30 miRNAs that were upregulated in blood cells of melanoma patients as compared to blood cells of healthy controls. The tets set and the independent validation set of the melanoma samples showed a high correlation of fold changes (0.81). Applying hierarchical clustering and principal component analysis we found that blood samples of melanoma patients and healthy individuals can be well differentiated from each other based on miRNA expression analysis. Using a subset of 16 significant deregulated miRNAs, we were able to reach a classification accuracy of 97.4%, a specificity of 95% and a sensitivity of 98.9% by supervised analysis. MiRNA microarray data were validated by qRT-PCR. Conclusions: Our study provides strong evidence for miRNA expression signatures of blood cells as useful biomarkers for melanoma. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
107. GENOME-WIDE MICRORNA AND MRNA EXPRESSION PATTERN IN ALZHEIMER’S DISEASE.
- Author
-
Pichler, Sabrina, Gu, Wei, Hartl, Daniela, Gasparoni, Gilles, Leidinger, Petra, Keller, Andreas, Meese, Eckart, Mayhaus, Manuel, Hampel, Harald, and Riemenschneider, Matthias
- Published
- 2017
- Full Text
- View/download PDF
108. Molekulare Signaturen im Blut von Patienten mit Bronchialkarzinomen
- Author
-
Leidinger, Petra
- Abstract
Das Ziel der vorliegenden Arbeit war, die Etablierung komplexer Autoantikörper- und miRNA-Expressionsprofile in peripherem Blut. Mit Hilfe der Analyse von über 1800 Autoantigenen wurde die Genauigkeit für verschiedene vergleichende Klassifikationen bestimmt. Lungentumorpatienten konnten von gesunden Probanden mit einer Genauigkeit von 98% getrennt werden. Patienten mit niedrig-gradigen Lungentumoren wurden mit einer Genauigkeit von 93,41% von Gesunden getrennt. Eine Trennung von Patienten mit NSCLC bzw. SCLC von Gesunden erzielte Genauigkeiten über 95%. Die Trennung der Lungentumorpatienten von Patienten mit COPD erreichte eine Genauigkeit von 76,75%. COPD-Patienten wurden von Gesunden mit 97,60% Genauigkeit getrennt. Entsprechende Unterscheidungen bei diesen Erkrankungen waren mit anderen Biomarkern bisher nicht mit vergleichbaren Ergebnissen möglich. Durch die Expressionsanalyse von über 800 miRNAs konnten Lungentumorpatienten mit einer Genauigkeit von 95,4% von Gesunden unterschieden werden. Insgesamt wurden in Blutzellen von Lungentumorpatienten 27 signifikant deregulierte miRNAs identifiziert. Mit vorliegender Studie konnten erstmals solide Tumoren anhand eines miRNA-Expressionsprofils in Blutzellen klassifiziert werden. In vorliegender Arbeit konnten komplexe Autoantikörper- und miRNA-Expressionsprofile in peripherem Blut von Lungentumorpatienten etabliert werden. Mit diesen Profilen wurden vergleichende Klassifikationen mit hoher Genauigkeit erreicht.
- Published
- 2009
109. TRPC1- and TRPC3-dependent Ca 2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo.
- Author
-
Belkacemi T, Niermann A, Hofmann L, Wissenbach U, Birnbaumer L, Leidinger P, Backes C, Meese E, Keller A, Bai X, Scheller A, Kirchhoff F, Philipp SE, Weissgerber P, Flockerzi V, and Beck A
- Subjects
- Animals, Astrocytes pathology, Brain Edema etiology, Brain Edema metabolism, Brain Edema pathology, Cell Movement physiology, Cell Proliferation physiology, Cerebral Cortex metabolism, Cerebral Cortex pathology, Disease Models, Animal, Gliosis etiology, Gliosis pathology, HEK293 Cells, Humans, Male, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Knockout, TRPC Cation Channels genetics, TRPC6 Cation Channel, Wounds, Stab metabolism, Wounds, Stab pathology, Astrocytes metabolism, Calcium Signaling physiology, Cerebral Cortex injuries, Gliosis metabolism, TRPC Cation Channels metabolism
- Abstract
Following brain injury astrocytes change into a reactive state, proliferate and grow into the site of lesion, a process called astrogliosis, initiated and regulated by changes in cytoplasmic Ca
2+ . Transient receptor potential canonical (TRPC) channels may contribute to Ca2+ influx but their presence and possible function in astrocytes is not known. By RT-PCR and RNA sequencing we identified transcripts of Trpc1, Trpc2, Trpc3, and Trpc4 in FACS-sorted glutamate aspartate transporter (GLAST)-positive cultured mouse cortical astrocytes and subcloned full-length Trpc1 and Trpc3 cDNAs from these cells. Ca2+ entry in cortical astrocytes depended on TRPC3 and was increased in the absence of Trpc1. After co-expression of Trpc1 and Trpc3 in HEK-293 cells both proteins co-immunoprecipitate and form functional heteromeric channels, with TRPC1 reducing TRPC3 activity. In vitro, lack of Trpc3 reduced astrocyte proliferation and migration whereas the TRPC3 gain-of-function moonwalker mutation and Trpc1 deficiency increased astrocyte migration. In vivo, astrogliosis and cortex edema following stab wound injury were reduced in Trpc3-/- but increased in Trpc1-/- mice. In summary, our results show a decisive contribution of TRPC3 to astrocyte Ca2+ signaling, which is even augmented in the absence of Trpc1, in particular following brain injury. Targeted therapies to reduce TRPC3 channel activity in astrocytes might therefore be beneficial in traumatic brain injury., (© 2017 Wiley Periodicals, Inc.)- Published
- 2017
- Full Text
- View/download PDF
110. EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research.
- Author
-
Van Deun J, Mestdagh P, Agostinis P, Akay Ö, Anand S, Anckaert J, Martinez ZA, Baetens T, Beghein E, Bertier L, Berx G, Boere J, Boukouris S, Bremer M, Buschmann D, Byrd JB, Casert C, Cheng L, Cmoch A, Daveloose D, De Smedt E, Demirsoy S, Depoorter V, Dhondt B, Driedonks TA, Dudek A, Elsharawy A, Floris I, Foers AD, Gärtner K, Garg AD, Geeurickx E, Gettemans J, Ghazavi F, Giebel B, Kormelink TG, Hancock G, Helsmoortel H, Hill AF, Hyenne V, Kalra H, Kim D, Kowal J, Kraemer S, Leidinger P, Leonelli C, Liang Y, Lippens L, Liu S, Lo Cicero A, Martin S, Mathivanan S, Mathiyalagan P, Matusek T, Milani G, Monguió-Tortajada M, Mus LM, Muth DC, Németh A, Nolte-'t Hoen EN, O'Driscoll L, Palmulli R, Pfaffl MW, Primdal-Bengtson B, Romano E, Rousseau Q, Sahoo S, Sampaio N, Samuel M, Scicluna B, Soen B, Steels A, Swinnen JV, Takatalo M, Thaminy S, Théry C, Tulkens J, Van Audenhove I, van der Grein S, Van Goethem A, van Herwijnen MJ, Van Niel G, Van Roy N, Van Vliet AR, Vandamme N, Vanhauwaert S, Vergauwen G, Verweij F, Wallaert A, Wauben M, Witwer KW, Zonneveld MI, De Wever O, Vandesompele J, and Hendrix A
- Subjects
- Biomedical Research, Databases, Bibliographic, Extracellular Vesicles physiology, Internationality
- Abstract
We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments. To achieve this, we describe EV-TRACK, a crowdsourcing knowledgebase (http://evtrack.org) that centralizes EV biology and methodology with the goal of stimulating authors, reviewers, editors and funders to put experimental guidelines into practice.
- Published
- 2017
- Full Text
- View/download PDF
111. High-throughput qRT-PCR validation of blood microRNAs in non-small cell lung cancer.
- Author
-
Leidinger P, Brefort T, Backes C, Krapp M, Galata V, Beier M, Kohlhaas J, Huwer H, Meese E, and Keller A
- Subjects
- Aged, Biomarkers, Tumor blood, Carcinoma, Non-Small-Cell Lung blood, Case-Control Studies, Cohort Studies, Female, Gene Expression Profiling, High-Throughput Screening Assays, Humans, Lung Neoplasms blood, Male, MicroRNAs blood, Middle Aged, Prognosis, Pulmonary Disease, Chronic Obstructive blood, RNA, Messenger genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Biomarkers, Tumor genetics, Carcinoma, Non-Small-Cell Lung genetics, Lung Neoplasms genetics, MicroRNAs genetics, Pulmonary Disease, Chronic Obstructive genetics
- Abstract
Validation of biomarkers is essential to advance the translational process to clinical application. Although there exists an increasing number of reports on small non-coding RNAs (microRNAs) as minimally-invasive markers from blood, serum or plasma, just a limited number is verified in follow-up studies. We used qRT-PCR to evaluate a known miRNA signature measured from blood that allowed for separation between patients with non-small cell lung cancer (NSCLC), COPD and healthy controls.From the data of our previous microarray studies we selected a panel of 235 miRNAs related to lung cancer and COPD. We observed a high concordance between the AUC values of our initial microarray screening and the qRT-PCR data (correlation of 0.704, p < 10-16). Overall, 90.3% of markers were successfully validated. Among the top markers that were concordant between both studies we found hsa-miR-20b-5p, hsa-miR-20a-5p, hsa-miR-17-5p, and hsa-miR-106a-5p. The qRT-PCR analysis also confirmed that non-small cell lung cancer patients could be accurately differentiated from unaffected controls: a subset of five markers was sufficient to separate NSCLC patients from unaffected controls with accuracy of 94.5% (specificity and sensitivity of 98% and 91%). Beyond differentiation from controls, we also succeeded in separating NSCLC patients from patients with COPD. MiRNAs that were identified as relevant for the separation between lung cancer and COPD by both qRT-PCR and the array-based studies included hsa-miR-26a-5p, hsa-miR-328-3p and hsa-miR-1224-3p. Although for differentiation between NSCLC patients from COPD patients more markers were required, still high accuracy rates were obtained (5 markers: 78.8%; 10 markers: 83.9%; 50 markers: 87.6%).
- Published
- 2016
- Full Text
- View/download PDF
112. Treatment-independent miRNA signature in blood of Wilms tumor patients.
- Author
-
Schmitt J, Backes C, Nourkami-Tutdibi N, Leidinger P, Deutscher S, Beier M, Gessler M, Graf N, Lenhof HP, Keller A, and Meese E
- Subjects
- Case-Control Studies, Child, Preschool, Cluster Analysis, Humans, Wilms Tumor drug therapy, MicroRNAs blood, MicroRNAs genetics, Transcriptome drug effects, Wilms Tumor blood, Wilms Tumor genetics
- Abstract
Background: Blood-born miRNA signatures have recently been reported for various tumor diseases. Here, we compared the miRNA signature in Wilms tumor patients prior and after preoperative chemotherapy according to SIOP protocol 2001., Results: We did not find a significant difference between miRNA signature of both groups. However both, Wilms tumor patients prior and after chemotherapy showed a miRNA signature different from healthy controls. The signature of Wilms tumor patients prior to chemotherapy showed an accuracy of 97.5% and of patients after chemotherapy an accuracy of 97.0%, each as compared to healthy controls., Conclusion: Our results provide evidence for a blood-born Wilms tumor miRNA signature largely independent of four weeks preoperative chemotherapy treatment.
- Published
- 2012
- Full Text
- View/download PDF
113. miRTrail--a comprehensive webserver for analyzing gene and miRNA patterns to enhance the understanding of regulatory mechanisms in diseases.
- Author
-
Laczny C, Leidinger P, Haas J, Ludwig N, Backes C, Gerasch A, Kaufmann M, Vogel B, Katus HA, Meder B, Stähler C, Meese E, Lenhof HP, and Keller A
- Subjects
- Animals, Humans, Internet, Mice, MicroRNAs genetics, MicroRNAs metabolism, MicroRNAs physiology, RNA, Messenger genetics, RNA, Messenger metabolism, Transcriptome, Zebrafish, Computers, Gene Expression Regulation, Melanoma genetics, Skin Neoplasms genetics
- Abstract
Background: Expression profiling provides new insights into regulatory and metabolic processes and in particular into pathogenic mechanisms associated with diseases. Besides genes, non-coding transcripts as microRNAs (miRNAs) gained increasing relevance in the last decade. To understand the regulatory processes of miRNAs on genes, integrative computer-aided approaches are essential, especially in the light of complex human diseases as cancer., Results: Here, we present miRTrail, an integrative tool that allows for performing comprehensive analyses of interactions of genes and miRNAs based on expression profiles. The integrated analysis of mRNA and miRNA data should generate more robust and reliable results on deregulated pathogenic processes and may also offer novel insights into the regulatory interactions between miRNAs and genes. Our web-server excels in carrying out gene sets analysis, analysis of miRNA sets as well as the combination of both in a systems biology approach. To this end, miRTrail integrates information on 20.000 genes, almost 1.000 miRNAs, and roughly 280.000 putative interactions, for Homo sapiens and accordingly for Mus musculus and Danio rerio. The well-established, classical Chi-squared test is one of the central techniques of our tool for the joint consideration of miRNAs and their targets. For interactively visualizing obtained results, it relies on the network analyzers and viewers BiNA or Cytoscape-web, also enabling direct access to relevant literature. We demonstrated the potential of miRTrail by applying our tool to mRNA and miRNA data of malignant melanoma. MiRTrail identified several deregulated miRNAs that target deregulated mRNAs including miRNAs hsa-miR-23b and hsa-miR-223, which target the highest numbers of deregulated mRNAs and regulate the pathway "basal cell carcinoma". In addition, both miRNAs target genes like PTCH1 and RASA1 that are involved in many oncogenic processes., Conclusions: The application on melanoma samples demonstrates that the miRTrail platform may open avenues for investigating the regulatory interactions between genes and miRNAs for a wide range of human diseases. Moreover, miRTrail cannot only be applied to microarray based expression profiles, but also to NGS-based transcriptomic data. The program is freely available as web-server at mirtrail.bioinf.uni-sb.de.
- Published
- 2012
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.