181 results on '"Laura McLaughlin"'
Search Results
102. B-lymphocytes support and regulate indirect T-cell alloreactivity in individual patients with chronic antibody-mediated rejection
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Robert I. Lechler, Jack Galliford, Jingyue Zhao, Vikki Semik, H. Terence Cook, Maria P. Hernandez-Fuentes, David Taube, Irene Rebollo-Mesa, Laura McLaughlin, Kin Yee Shiu, Candice Roufosse, Anthony Dorling, Robert W. Bowers, and Paul Brookes
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Graft Rejection ,lymphocytes ,Enzyme-Linked Immunospot Assay ,Isoantigens ,medicine.medical_treatment ,T cell ,Biopsy ,T-Lymphocytes ,Cell Communication ,Kidney ,Lymphocyte Activation ,Peripheral blood mononuclear cell ,CD19 ,Article ,Immunophenotyping ,Interferon-gamma ,Immunity ,Isoantibodies ,medicine ,Humans ,IL-2 receptor ,Allorecognition ,Cells, Cultured ,B-Lymphocytes ,biology ,Kidney Transplantation ,cytokines ,Immunity, Humoral ,Cytokine ,medicine.anatomical_structure ,Phenotype ,Treatment Outcome ,Nephrology ,Immunology ,Chronic Disease ,biology.protein ,Interferon-gamma Release Tests ,transplantation - Abstract
We explored how B-lymphocytes influence in vitro T-cell alloresponses in patients with antibody-mediated rejection (AMR), testing whether B-cells would be preferentially involved in this group of patients. Peripheral blood mononuclear cells were collected from 65 patients having biopsy: 14 patients with AMR and 5 with no pathology on protocol; 38 with AMR and 8 with nonimmunologic damage on 'for cause'. Using enzyme-linked immunosorbent spot assays, we found interferon-γ production by indirect allorecognition in 45 of 119 total samples from the 65 patients. B-cells preferentially processed and presented donor alloantigens in samples from AMR patients. In a further 25 samples, B-cell-dependent allo-specific reactivity was shown by depletion of CD25(+) cells and these individuals had higher percentages of CD4CD25hi cells. In 21 samples, reactivity was shown by depletion of CD19(+) cells, associated with polarized cytokine production toward IL-10 after polyclonal activation by IgG/IgM. Overall, this shows a significant contribution by B-cells to indirect donor-specific T-cell reactivity in vitro in patients with AMR. Active suppression by distinct phenotypes of T- or B-cells in approximately half of the patients indicates that chronic AMR is not characterized by a universal loss of immune regulation. Thus, stratified approaches that accommodate the heterogeneity of cell-mediated immunity might be beneficial to treat graft dysfunction.
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- 2014
103. Solar ultraviolet-induced erythema in human skin and nuclear factor-kappa-B–dependent gene expression in keratinocytes are modulated by a French maritime pine bark extract
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Claude Saliou, Lester Packer, Jeongmin Lee, Gerald Rimbach, Saeed Hosseini, Laura McLaughlin, Hadi Moini, and Ronald R. Watson
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Adult ,Keratinocytes ,Male ,Transcriptional Activation ,Pathology ,medicine.medical_specialty ,Adolescent ,Erythema ,Cell Survival ,Ultraviolet Rays ,Anti-Inflammatory Agents ,Administration, Oral ,Human skin ,Pharmacology ,Biochemistry ,Cell Line ,Transactivation ,Genes, Reporter ,Physiology (medical) ,Gene expression ,medicine ,Humans ,Skin ,Flavonoids ,integumentary system ,Plant Extracts ,Chemistry ,NF-kappa B ,Dose-Response Relationship, Radiation ,DNA ,Dose–response relationship ,HaCaT ,medicine.anatomical_structure ,Cell culture ,Female ,medicine.symptom ,Keratinocyte ,Protein Binding ,Signal Transduction - Abstract
The procyanidin-rich French maritime pine bark extract Pycnogenol (PBE) has been investigated for its effect in protecting human skin against solar UV-simulated light-induced erythema. Twenty-one volunteers were given an oral supplementation of Pycnogenol: 1.10 mg/kg body weight (b. wt.)/d for the first 4 weeks and 1.66 mg/kg b. wt./d for the next 4 weeks. The minimal erythema dose (MED) was measured twice before supplementation (baseline MED), once after the first 4 weeks of supplementation, and a last time at the end of the study. The UVR dose necessary to achieve 1 MED was significantly increased during PBE supplementation. Since the activation of the pro-inflammatory and redox-regulated transcription factor NF-kappaB is thought to play a major role in UVR-induced erythema, the effect of PBE was also investigated in the human keratinocyte cell line HaCaT. PBE, added to the cell culture medium, inhibited UVR-induced NF-kappaB-dependent gene expression in a concentration-dependent manner. However, NF-kappaB-DNA-binding activity was not prevented, suggesting that PBE affects the transactivation capacity of NF-kappaB. These data indicate that oral supplementation of PBE reduces erythema in the skin. Inhibition of NF-kappaB-dependent gene expression by PBE possibly contributes to the observed increase in MED.
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- 2001
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104. Familism and Latino adolescent depressive symptoms: The role of maternal warmth and support and school support
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Cupito, Alexandra M., Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Supple, Andrew "Andy", Cupito, Alexandra M., Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., and Supple, Andrew "Andy"
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Objectives: This study examined the relationship between familism and depressive symptoms across relational contexts in adolescence, and whether maternal warmth and support, and school support moderated the relationship between familism and depressive symptoms. Method: A total of 180 Latino adolescents (53% female) in 7th through 10th grades (average age = 14 years) participated in this cross-sectional study. The adolescents lived in an emerging Latino community in a rural area in the U.S. South. Most of the adolescents were Mexican-origin (78%) and born in the United States (60%), while the vast majority of their parents were foreign born (95%). Results: Overall, familism was associated with fewer adolescent depressive symptoms. School support moderated the relationship between familism and adolescent depressive symptoms such that familism’s protective effect was only evident when adolescents reported low levels of school support. In the context of average to high school support, adolescents reported low depressive symptoms regardless of familism. However, maternal warmth and support failed to moderate the relationship. Conclusions: Familism may be most protective for adolescents not feeling supported at school, suggesting that these values may offset the risk of a risky school environment.
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- 2016
105. Secondary Traumatic Stress for Trauma Researchers: A Mixed Methods Research Design
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Gonzalez, Laura McLaughlin, Murphy, Arthur D., NC DOCKS at The University of North Carolina at Greensboro, Gonzalez, Laura McLaughlin, Murphy, Arthur D., and NC DOCKS at The University of North Carolina at Greensboro
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Forty-nine infants and toddlers were killed and 93 others were injured in the ABC Day Care Center fire disaster in Hermosillo, Mexico. This study describes the experiences of ten mental health professionals who researched the community-scale grief and provided clinical services to the parents and caregivers of the affected children. A concurrent triangulation mixed-methods approach was used to quantitatively measure and qualitatively understand the secondary traumatic stress (STS) of the responding professionals. Results indicated that experiences of STS decreased with time and debriefing, however, the responding professionals who were more directly connected to the trauma and those who worked with parents who lost their children displayed an enduring impact of STS. Coping strategies and gender expectations are considered in a discussion of debriefing in the specific cultural context of Northwestern Mexico.
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- 2016
106. Linking multicultural counseling and social justice through advocacy
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
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The concepts of multicultural counseling, social justice, and advocacy may be utilized without a clear sense of how best to operationalize them in counselor training. In this article, the authors offer a perspective on how advocacy and social justice interrelate and share strategies for infusing advocacy into counselor training to achieve social justice goals. The authors provide six experiential activities counselor educators may use to provide counselors-in-training experience in a range of advocacy skills.
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- 2016
107. Acculturation conflict in Latino youth: Discrimination, ethnic identity, and depressive symptoms
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Gonzalez, Laura McLaughlin, Huq, Nadia, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Gonzalez, Laura McLaughlin, Huq, Nadia, NC DOCKS at The University of North Carolina at Greensboro, and Stein, Gabriela L.
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Objectives: Patterns of parent–adolescent conflict differ between immigrant and nonimmigrant families living in the United States (Fuligni, 1998). Despite this, there is limited empirical literature examining the nuanced nature of parent–adolescent conflict in immigrant families. To fill this gap, the current study examined the role of 2 types of conflict (i.e., general and acculturation) in predicting psychosocial outcomes (i.e., depressive symptoms and ethnic identity) among Latino adolescents, and whether these relationships differ within the context of peer discrimination. Method: All survey administration was completed in the participating school’s cafeteria. The sample consisted of 7th through 10th graders (n = 172) with a mean age of 14.01 years (SD = 1.32.) The sample consisted of 53% females, and was primarily Mexican in origin (78%). Results: As hypothesized, parent–adolescent acculturation conflict uniquely predicted greater depressive symptoms and lower ethnic private regard, even when controlling for parent–adolescent general conflict. However, acculturation conflict predicted lower ethnic private regard only in the presence of greater peer discrimination. More specifically, peer discrimination moderated the relation between acculturation conflict and ethnic private regard such that adolescents who reported the highest levels of acculturation conflict and peer discrimination reported the lowest levels of ethnic private regard. Conclusions: These results suggest that for Latino youth and their families, acculturation conflict may be particularly problematic, as compared with general conflict. In addition, youth who face ethnicity-based stressors in both familial and school contexts are especially at risk in their ethnic identity development.
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- 2016
108. Using evidence-based practice in the school health room
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Laura McLaughlin and Margaret W. Bultas
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education.field_of_study ,Evidence-based practice ,Isolation (health care) ,business.industry ,media_common.quotation_subject ,Best practice ,education ,Population ,MEDLINE ,General Medicine ,Evidence-Based Nursing ,Nursing ,Practice Guidelines as Topic ,School Nursing ,Medicine ,Humans ,Quality (business) ,School health ,Set (psychology) ,business ,Child ,media_common - Abstract
School nurses should be engaging in evidence-based practice (EBP) in their health room settings as supported by the initiatives of the Institute of Medicine. EBP is a set of skills that health professionals can use in order to ensure high quality and current clinical care is provided. School nurses are faced with a diverse and complex population of children and often work in isolation from nursing peers, which can lead to limited professional peer discussions about recent changes or outdated clinical practices. EBP combines the use of evidence from the literature, expert guidelines, clinician expertise, and client preferences in order to provide optimum care. This article leads the school nurse through the process and use of EBP in the school setting.
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- 2013
109. Expert consensus on the nutritional therapy for patients with malignancies
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Laura, McLaughlin
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- 2013
110. Quality of Life and Taste Dysfunction in Head and Neck Cancer Treatment Survivors
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Leslie Hinyard, S Mahon, and Laura McLaughlin
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Oncology ,medicine.medical_specialty ,Taste ,Quality of life (healthcare) ,business.industry ,Health Policy ,Internal medicine ,Head and neck cancer ,Public Health, Environmental and Occupational Health ,medicine ,medicine.disease ,business - Published
- 2016
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111. Taste dysfunction in head and neck cancer survivors
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Laura McLaughlin
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Oncology ,Male ,medicine.medical_specialty ,Taste ,Sensory system ,Taste Disorders ,Postoperative Complications ,Weight loss ,Internal medicine ,Outpatients ,medicine ,Prevalence ,Humans ,Survivors ,Radiation Injuries ,Aged ,business.industry ,Head and neck cancer ,Weight change ,Oncology Nursing ,Cancer ,Taste Perception ,Middle Aged ,medicine.disease ,Dysgeusia ,Cross-Sectional Studies ,Head and Neck Neoplasms ,Taste function ,Female ,medicine.symptom ,business - Abstract
PURPOSE/OBJECTIVES To describe the prevalence of issues with taste function in survivors of head and neck cancer. DESIGN Exploratory, cross-sectional. SETTING Outpatients from Saint Louis University Cancer Center in Missouri. SAMPLE 92 adult head and neck cancer survivors, heterogeneous in cancer site, treatment type, and time post-treatment, ranging from three months to more than 28 years after completion of therapy. METHODS Taste discrimination was assessed using high, medium, and low concentrations of sweet, salty, sour, and bitter tasting solutions. MAIN RESEARCH VARIABLES Taste, percentage of weight change, tumor site and stage, treatment type, and time since completion of therapy. FINDINGS Eighty-five of 92 participants had some measurable taste dysfunction. Confusion between bitter and sour and the inability to discriminate among the different concentrations of the sweet solutions were common. Statistically significant weight loss was associated with dysgeusia. CONCLUSIONS Taste dysfunction was a persistent problem across all categories of head and neck cancer treatments, sites, and stages. Participants who reported the loss of one or more specific taste modality performed poorly on the taste test. However, participants could not accurately predict which taste was most severely impaired. IMPLICATIONS FOR NURSING Taste dysfunction is a long-term treatment-related side effect for head and neck cancer survivors. Assessing for taste changes and dysgeusia are important nursing considerations, as taste loss is distressing and associated with decreased appetite. Future studies are needed to identify interventions to help patients better manage and adapt to this long-term complication of cancer therapy. KNOWLEDGE TRANSLATION Flavors are recognized by taste, texture, aroma, thermal quality, and visual cues. A disruption of one or more of those sensory experiences alters flavor recognition. Having intact taste sense but impaired flavor recognition is possible. Finally, taste is not accurately self-reported because it is commonly confused with flavor recognition.
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- 2012
112. Understanding taste dysfunction in patients with cancer
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Laura McLaughlin and Suzanne M. Mahon
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medicine.medical_specialty ,Taste ,media_common.quotation_subject ,Psychological intervention ,Sensory system ,Antineoplastic Agents ,Taste Disorders ,Education, Nursing, Continuing ,Tongue ,Neoplasms ,Nursing Interventions Classification ,Medicine ,Humans ,In patient ,Intensive care medicine ,General Environmental Science ,media_common ,business.industry ,Cancer ,Appetite ,medicine.disease ,General Earth and Planetary Sciences ,Digestive tract ,business ,Nutritive Value - Abstract
Taste dysfunction is a significant but underestimated issue for patients with cancer. Impaired taste results in changes in diet and appetite, early satiety, and impaired social interactions. Nurses can play a key role in educating patients and families on the pathophysiology of taste dysfunction by suggesting interventions to treat the consequences of taste dysfunction, when available, and offering psychosocial support as patients cope with this often devastating consequence of treatment. Taste recognition helps humans identify the nutritional quality of food and signals the digestive tract to begin secreting enzymes. Spoiled or tainted foods typically are recognized by their bad taste. Along with the other sensory systems, taste is crucial for helping patients treated for cancer feel normal. This article will review the anatomy and physiology of taste; define the different types of taste dysfunction, including the underlying pathophysiologic basis related to cancer treatment; and discuss potential nursing interventions to manage the consequences of taste dysfunction.
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- 2012
113. Spanish-Speaking Immigrant Parents and Their Children: Reflections on the Path to College
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Borders, L. DiAnne, Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Borders, L. DiAnne, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
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Immigrant parents in a new cultural setting may experience both structural and individual barriers, which complicate the process of helping their children plan for college. Focus group interviews were conducted with 15 Spanish-speaking immigrant parents to highlight their perceptions and experiences. Critical humanism frames a counseling response to these concerns.
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- 2015
114. Changes in academic aspirations and expectations among Asian American adolescents
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, and Stein, Gabriela L.
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Aspirations and expectations are critical to academic attainment and success, yet little is known about how they change over the high school years. With longitudinal data from 157 Asian American adolescents (60% female), we examined normative and within-person changes in aspirations and expectations, and associations with psychological adjustment. Results suggest that, at the group level, aspirations are relatively stable, but expectations increase over time. At the intraindividual level, higher family obligation attitudes were associated with higher aspirations reported from year to year. Higher ethnic centrality was marginally related to higher expectations, whereas higher perceived discrimination was associated with lower expectations. Although expectations were associated with higher self-esteem, aspirations had adverse associations with well-being. Closer alignment of aspirations with expectations was associated with healthier psychosocial outcomes.
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- 2015
115. Supervision in Spanish: Reflections from supervisor-trainee dyads
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
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Due to the increasing presence of clients who speak a language other than English, educators and supervisors will need to address bilingual training issues in mental health service provision. Supervision that utilizes the preferred language of the client is an effective way to support growth in bilingual trainees, but there is little scholarly literature describing best practices or approaches to this complex task. We describe our experiences with bilingual supervision in Spanish (from both trainee and supervisor points of view) and provide resources and suggestions to encourage other supervisory dyads to attempt bilingual service provision.
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- 2015
116. “It takes fire to make steel”: Stories of two African American males finding purpose through their college experiences
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Borders, L. DiAnne, Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Borders, L. DiAnne, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
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Purpose – This study aims to understand the asset and success factors that contributed to college completion of African American males who persisted through college. Only a dismal 22 per cent of African American males receive bachelor’s degrees compared to 41 per cent of White males (Kena et al., 2015).Design/methodology/approach – The data were analyzed using interpretive phenomenological analysis. The authors interviewed two first-generation African-American males from rural backgrounds to capture their experiences of their process to college completion.Findings – Themes, based in cultural capital theory, that impacted their college persistence were identified within their pre-college experiences, college experiences and post-college perceptions. Recommendations for helping rural African-American males attend and persist through college are offered.Research limitations/implications – Only two participants from one predominately white institution in the southeastern USA were interviewed. Rural students from other geographical areas might have different backgrounds, challenges, assets and successes. Although the interview questions were based on relevant literature, they may not have covered all key aspects of the participants’ experiences. As in any qualitative study, biases of the researchers and research team may have influenced the results, although these were identified and shared before reading any of the transcripts and then discussed several times during the data analysis process.Practical implications – Educators not only should try to address the cultural capital limitations of these men but also highlight and build on their cultural assets. These assets include familial and platonic individuals who see their potential for success and encourage them to attend college to become something better than what they see in their community, reverse role models who encourage youth to make different choices than they did, media-based examples of successful Black stud
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- 2015
117. Barriers to college access for Latino adolescents: A comparison of existing theoretical frameworks
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
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A comprehensive description of barriers to college access for Latino/a adolescents is an important step toward improving educational outcomes. However, relevant scholarship on barriers has not been synthesized in a way that promotes coherent formulation of intervention strategies or constructive scholarly discussion. The goal of this article is to synthesize the barriers literature briefly as a basis for comparing and evaluating possible frameworks for addressing barriers. The bridging multiple worlds model, social capital theory, and social cognitive career theory are 3 possible frameworks for addressing barriers to college access for Latinos/as. Implications are given for future barriers research and programmatic interventions.
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- 2015
118. Using Voice-Recorded Reflections to Increase Cognitive Presence in Hybrid Courses
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Taddei, Laura McLaughlin, primary and Budhai, Stephanie Smith, additional
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- 2015
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119. Bulimia and interpersonal relationships: A longitudinal study
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Laura McLaughlin Mann, Mark H. Thelen, Julie Pruitt, and Janet E. Farmer
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Longitudinal study ,Social Psychology ,Behavior change ,Follow up studies ,General Medicine ,behavioral disciplines and activities ,Social relation ,Developmental psychology ,Psychiatry and Mental health ,Clinical Psychology ,Interpersonal relationship ,mental disorders ,Psychological counseling ,Psychology ,Male-female relations ,Subclinical infection - Abstract
We assessed changes in bulimia in female college students and changes in the relation between bulimia and interpersonal relationships with women and men. A measure of bulimia (BULIT) was taken at Time 1, after a 7-month interval (Time 2), and again after a 12-month interval (Time 3). Measures of interpersonal relationships with women and men were taken at Time 2 and Time 3. Three groups were constituted on the basis of BULIT scores at Time 1: bulimic, subclinical bulimic (Ss who tested just under the cutoff for bulimia), and normal
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- 1990
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120. HLA typing by NGS in the clinical lab: our one and a half years experience
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Curt Lind, Jamie L. Duke, Dimitri S. Monos, Deborah Ferriola, Allison Gasiewski, Anna Papazoglou, Rita Walker, Marianne Rogers, Laura McLaughlin, Larissa Slavich, Steve Heron, Anh Huynh, Yanping Huang, and Hilary Mehler
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Genetics ,Concordance ,Immunology ,Genotype ,Immunology and Allergy ,Locus (genetics) ,General Medicine ,Human leukocyte antigen ,Typing ,Biology ,Amplicon ,Genotyping ,DNA sequencing - Abstract
Aim We present our experience using the Holotype™ Kit by Omixon for next generation sequencing (NGS) of HLA genes in a clinical setting for one and a half years, starting December 2013. Methods Samples were prepared using the X4 Holotype™ kit by Omixon for up to 7 loci (full length HLA-A, B, C, DQA1 and DQB1, intron 1 to intron 4 of DRB1, and intron 1–3’ UTR of DPB1) and were sequenced in both an individual and pooled amplicon approach using 2 × 251 bp reads on the Illumina MiSeq. Samples were also characterized in parallel with a second method (Sanger SBT, SSO, or SSP) to confirm the genotyping results by NGS and identify possible causes of discrepancies, if any. Two analysis programs were used to genotype the NGS data, Target by Omixon (v 1.7/1.8) and NGSengine by GenDX (v 1.3/1.6). Results A total of 1046 samples have been sequenced: 497 (47%) related to bone marrow transplant cases, 250 (24%) related to solid organ transplantation, and 299 (29%) samples being either proficiency, outreach or disease association studies. Approximately 975 typings were performed for HLA-A, B, C, DRB1 and DQB1, and 600 typings for DQA1 and DPB1. On average, one sequencing run was performed per week with 12 samples on the MiSeq for a total of 83 runs utilizing both nano and full flow cells. At best, the turnaround time from amplification to analysis completion was 4 days, but averages 10 days due to the laboratory workflow and a single sequencing run per week. Concordance was 99.7% between NGS and secondary typing methods (SBT/SSP/SSOP). Discordance was due to incorrect or incomplete (ambiguous) SBT/SSP typing, allele imbalance, NGS genotyping software errors, incorrect reporting of results and new alleles. Two genotyping programs were used for analysis to safeguard against systematic analysis errors and agree for 94.8% of the best match allele calls. Ambiguous results are most common at the DPB1 locus due to a lack of phasing between exons 2 and 3 or the unsequenced exon 1 (17% of alleles) and the DRB1 locus due to not sequencing exon 1 (3.8% of alleles). No ambiguities were detected among the other loci. Conclusions We have genotyped over 1000 samples using NGS, with a very high concordance rate with other HLA typing methods and low ambiguity rates for DRB1 and DPB1 as described above. This technology is therefore very appropriate for HLA typing in a clinical setting. D. Monos: Grant/Research Support; Company/Organization; Omixon. 7. Other (Identify); Company/Organization; Royalty from Omixon.
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- 2015
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121. Enhanced characterization of 109 genomic dna reference material for 6 HLA loci by next-generation sequencing (NGS)
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Lisa V. Kalman, Dimitri S. Monos, Jamie L. Duke, Anh Huynh, Lawrence J. Jennings, Maria Bettinotti, Alison Gasiewski, Deborah Ferriola, Laura McLaughlin, Marianne Rogers, and Larissa Slavich
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Genetics ,Sanger sequencing ,education.field_of_study ,medicine.diagnostic_test ,Immunology ,Population ,General Medicine ,Human leukocyte antigen ,Biology ,DNA sequencing ,genomic DNA ,symbols.namesake ,medicine ,symbols ,Immunology and Allergy ,Typing ,Allele ,education ,Genetic testing - Abstract
Aim Provide complete, unambiguous characterization of 6 HLA loci (HLA-A, B, C, DPB1, DQB1, and DRB1) for 109 publicly available cell lines from Coriell Cell Repository, in collaboration with the Center for Disease Control and Prevention’s Genetic Testing Reference Material program (GeT-RM). These cell lines have previously been characterized for 5 pharmacogenetic (PGx) genes (Pratt, V et al J Mol Diag 2010 12(6):835–846) and HLA genes using multiple assays and are intended for use as reference materials for clinical genetic testing laboratories. Method DNA samples were amplified at 6 HLA loci using Omixon Holotype primers delineating full length genes (5’UTR to 3’UTR) with the exception of DRB1 (intron 1 to intron 4). Sequencing libraries were prepared using Holotype X4 kits for NGS on the Illumina MiSeq. Target (Omixon) and NGSengine (GenDX) were used to analyze the NGS data and results were compared to HLA typing generated by Sanger sequencing (SBT) using SSO and SSP to resolve ambiguities. Results 187 unique alleles were identified. The table below indicates the percent of people in the given population that will carry one of the alleles included in this study (Maiers, M et al Hum Imunol 2010 68(9):779–788). DPB1 frequencies were not available from this source. Download : Download full-size image We observed 98 5% concordance between HLA typing results using SBT and NGS, with discordance caused by sequence differences outside regions characterized by SBT (n = 21), and errors in SBT/SSP/SSO typing (n = 2). Ambiguities persist in 32/1308 allele calls, with the majority due to alternative cis/trans combinations of exons 2 and 3 in DPB1 (n = 30) and 2 caused by polymorphisms in the unsequenced exon 1 of the DRB1 gene. Conclusion NGS enhanced the characterization of alleles in this study by identifying errors and allowing analysis of regions not examined by other typing methods. These samples will be useful for assay development and validation, quality control and proficiency testing, and should help to improve the accuracy of PGx and HLA testing in clinical laboratories. D. Monos: Other (Identify); Company/Organization; Royalty.
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- 2015
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122. Mapping of immune responses following wild-type and mutant ABeta42 plasmid or peptide vaccination in different mouse haplotypes and HLA Class II transgenic mice
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Yun Bai, Laura McLaughlin, Kenneth E. Ugen, David B. Weiner, Alex Whelan, Vera Saulino, HuiQin Dong, Andrew Y. Choo, Chuanhai Cao, Philip Y. Choe, and Michele A. Kutzler
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animal diseases ,Molecular Sequence Data ,Enzyme-Linked Immunosorbent Assay ,Mice, Transgenic ,Lymphocyte proliferation ,Biology ,CD8-Positive T-Lymphocytes ,Epitope ,Lymphocyte Depletion ,Mice ,Immune system ,mental disorders ,medicine ,Animals ,Amino Acid Sequence ,Cloning, Molecular ,B cell ,Amyloid beta-Peptides ,General Veterinary ,General Immunology and Microbiology ,Sequence Homology, Amino Acid ,ELISPOT ,Public Health, Environmental and Occupational Health ,Histocompatibility Antigens Class II ,DNA ,Virology ,Vaccination ,Mice, Inbred C57BL ,Infectious Diseases ,medicine.anatomical_structure ,Immunization ,Haplotypes ,Immunology ,Antibody Formation ,Peptide vaccine ,Molecular Medicine ,Peptides ,Plasmids - Abstract
Although the recent clinical trial of the ABeta42 peptide vaccine against Alzheimer's Disease (AD) has been halted due to adverse events, the apparent clinical utility of this approach underscores the need to further improve the safety of the vaccine, as well as to understand the potential immunological basis for complications. In this study, we examine both humoral and cellular immune responses elicited by immunization with peptide or DNA encoding wild-type and the Flemish and Dutch mutations of ABeta42 (i.e. the beta amyloid peptide spanning amino acids 1-42) in mice of different immune haplotypes as well as HLA Class II transgenic mice. The Flemish and Dutch mutations have been associated with cerebrovascular hemorrhages in affected individuals. These data allow determination of potential immunological responses that could mediate pathology observed with mutant forms of amyloid beta, as well as lead to the generation of safer vaccine preparations. Following peptide or plasmid immunization, antibody responses were measured against the different ABeta42 peptides in an ELISA assay, while T cell epitopes were analyzed through interferon gamma ELISPOT and lymphocyte proliferation assays. B cell mapping studies indicated that sera from all of the haplotype mice vaccinated with any of the ABeta42 peptides reacted specifically to the first 10 amino acids of ABeta42 with the ABeta42 mutants eliciting higher immune responses. ELISPOT analysis, which accessed cellular immune responses indicated that mice expressed differences in Class I epitopes dependent on the different immune haplotypes. These results may have implications for the design of future ABeta42 based vaccines against Alzheimer's Disease.
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- 2005
123. Parental involvement in college planning: Cultural considerations when working with African American families
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Borders, L. DiAnne, Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Borders, L. DiAnne, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
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Purpose: The purpose of this article was to describe Hossler and Gallagher’s (1987) college choice model and emphasize the predisposition phase of the model as the starting point for school counselors’ efforts to help African American parents foster their children’s college planning in the college choice process.Design/methodology/approach: The authors wrote this manuscript as a conceptual approach to helping school counselors work with African American parents in their children’s college planning process by including two case studies as examples.Findings: This is a conceptual article.Practical implications: School counselors should be culturally competent and aware of how African Americans reartheir children to help them successfully navigate college planning. For example, schoolcounselors can learn about and share information with families about colleges that have supportprograms assisting African American students toward college completion.Originality/value: This paper is important to the field of education as it contributes to the literature regarding how school counselors can assist students in becoming college and career ready by working with their parents using a college choice model.
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- 2014
124. School community engaging with immigrant youth: Incorporating personal/social development and ethnic identity development
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Eades, Mark P., Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Supple, Andrew "Andy", Eades, Mark P., Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, and Supple, Andrew "Andy"
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It has been projected that 33% of all school children will be from immigrant households by the year 2040 (Suarez-Orozco et al., 2010). For school personnel (e.g., administrators, counselors, teachers) working with immigrant youth and adolescents, understanding ethnic identity development is an essential cultural competency. In this essay, the authors outline how the family, peer, and school contexts can influence a student’s ethnic identity, along with suggested activities that utilize ethnic identity development to enhance student personal/social development. Greater personal/social development of individual students and greater integration of marginalized ethnic groups can contribute to a healthier school community. Informal methods of evaluating outcomes are also identified.
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- 2014
125. Familial Cultural Values, Depressive Symptoms, School Belonging and Grades in Latino Adolescents: Does Gender Matter?
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, and Stein, Gabriela L.
- Abstract
Past studies document that Latino familial cultural values (i.e. familism, affiliative obedience and filial obligation) protect against depressive symptoms and promote academic resilience in adolescence. However, some studies suggest that familial cultural values differ across gender, with females reporting greater obligations and fewer freedoms compared to their male counterparts. We examined the relationship between familial cultural values, gender, depressive symptoms and school outcomes in a sample of 179 Latino adolescents (52.9 % female; mean age = 14). Females reported greater levels of familism and greater filial obligations. We also found greater familism to be associated with fewer depressive symptoms and greater sense of school belonging for both genders. Similarly, moderate levels of filial obligations were associated with better grades across genders. In contrast, filial obligation and affiliative obedience were associated with fewer depressive symptoms only for females. While these values serve an equally protective function in the academic adjustment of both females and males, familial cultural values may be uniquely protective for females against depressive symptoms. Effective interventions for Latino youth should capitalize on the protective and resilient effects of familial cultural values and be cognizant of the role gender plays in the relationship between these values and outcomes.
- Published
- 2014
126. The Impact of Discrimination and Support on Developmental Competencies in Latino Adolescents
- Author
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, and Stein, Gabriela L.
- Abstract
Discrimination is considered a contextual risk factor for ethnic minority youth, but social support provided in the same context may function to offset the risk or encourage adaptive responses. Per the integrative model of child development (Garcia Coll et al., 1996), experiences in the school, familial, and community settings can foster the development of cognitive, social, and emotional competencies in the presence of racism and discrimination. The current study evaluated how perceptions of discrimination and support in the school setting influenced school belonging, college-going self-efficacy, and depressive symptoms in a sample of Latino youth (N = 179). We distinguished between peers and nonparental adults as the sources of discrimination and support. Overall, peer support was associated with all three outcomes, suggesting peers as a positive resource. Adult support was only significant in the school belonging model. Findings supported a limited moderating role for peer support on peer discrimination, but only for the depressive symptoms outcome. Implications and suggestions for mental health practitioners are offered.
- Published
- 2014
127. The Latino Parents-Learning About College (LaP-LAC) Program: Educational Empowerment of Latino Families Through Psychoeducational Group Work
- Author
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Borders, L. DiAnne, Gonzalez, Laura McLaughlin, Hines, Erik M., NC DOCKS at The University of North Carolina at Greensboro, Borders, L. DiAnne, Gonzalez, Laura McLaughlin, Hines, Erik M., and NC DOCKS at The University of North Carolina at Greensboro
- Abstract
Parental involvement is crucial to facilitating a child's high school success and enhancing their post-secondary opportunities. Unfortunately, the ability for Latina/o parents and guardians to engage in parental involvement is hindered by a general lack of familiarity with U.S. educational systems and post-secondary options. With these barriers in mind, the Latino Parents-Learning About College (LaP-LAC) program was developed. The LaP-LAC is a psychoeducational group work experience wherein Latina/o parents with high school-aged children learn to understand the high school curriculum and become more familiar with post-secondary options (including financial aid), in an effort to empower themselves and their families.
- Published
- 2014
128. Ethnic Identity as a Protective Factor in the Lives of Asian American Adolescents
- Author
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Supple, Andrew "Andy", Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., and Supple, Andrew "Andy"
- Abstract
This study examined the buffering role of ethnic identity for Asian American adolescents living in an emerging immigrant community. A sample of 176 Asian American ninth graders completed self-report measures assessing ethnic identity, stressors (i.e., perceived racial/ethnic discrimination; economic stress), and mental health outcomes (i.e., depressive symptoms, self-esteem) at two time points a year apart. The study tested whether ethnic identity moderated the relationships between discrimination and economic stress and mental health outcomes. Ethnic identity did not buffer or exacerbate the relationship between discrimination and mental health outcomes, and discrimination was a consistent predictor of concurrent functioning. However, ethnic identity served both to buffer and to exacerbate in predicting mental health outcomes when youth reported high levels of economic stress. These findings suggest that it is important to further examine the role of ethnic identity for youth facing high levels of economic stress, especially in emerging immigrant communities.
- Published
- 2014
129. Self-awareness theory, family history of alcoholism, and adolescent alcohol involvement
- Author
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Chassin, Laurie, Mann, Laura McLaughlin, and Sher, Kenneth J.
- Subjects
Self-perception -- Psychological aspects ,Alcoholics -- Psychological aspects ,Alcohol and youth -- Psychological aspects ,Psychology and mental health - Published
- 1988
130. Human Papillomavirus and Head and Neck Cancer
- Author
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Stephanie Shorey, Laura McLaughlin, and Margaret W. Bultas
- Subjects
Advanced and Specialized Nursing ,Oncology ,Cervical cancer ,medicine.medical_specialty ,business.industry ,Incidence (epidemiology) ,Head and neck cancer ,HPV infection ,virus diseases ,medicine.disease ,Genital warts ,Internal medicine ,Medicine ,Sex organ ,Human papillomavirus ,business ,Oropharyngeal Cancers - Abstract
with human papillomavirus (HPV),1 which is highly transmissible and spreads from direct skin-to-skin contact.2 HPV is the leading cause of cervical cancer and genital warts, but the link between HPV infection and anal, penile, and oropharyngeal cancers is less understood.3 Each year, over 50,000 Americans are diagnosed with head and neck cancer (HNC).4 In recent years, oropharynx cancers have begun to increase at alarming rates, while the incidence of all other HNCs have declined.5 Oropharynx cancers are affecting middle-aged men of higher socioeconomic status with negligible smoking history.5,6 This trend is attributed to the emerging increased incidence of HPV-related oropharyngeal cancers.5 It is estimated that 90% of these cancers are related to HPV-16, the same strain responsible for the majority of cervical cancer cases.5,6 It is believed that most sexually active adults will contract HPV during their lifetime because infections are often asymptomatic.7 More than 100 strains of HPV have been identified and are divided into 2 categories: high-risk types, causing cancers, and low-risk types, causing genital warts.6 Infections with low-risk strains often resolve without intervention within 2 years.7 However, high-risk strains HPV-16 and HPV-18 are subject to mutations. In the laboratory, the potential for oncogenicity can be evaluated by flow cytometry. Mutation of HPV to E6 and E7 is highly indicative of its cancercausing potential.8
- Published
- 2012
- Full Text
- View/download PDF
131. The influence of cultural identity and perceived barriers on college preparation and aspirations of Latino youth in emerging immigrant communities.
- Author
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Gonzalez, Laura McLaughlin, Huq, Nadia, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Gonzalez, Laura McLaughlin, Huq, Nadia, NC DOCKS at The University of North Carolina at Greensboro, and Stein, Gabriela L.
- Abstract
Emerging immigrant communities differ from established communities in terms of needs and available resources. Students in these emerging communities may still be acculturating to new contexts and establishing their ethnic identities, which may impact their ability to engage in planning for the future. The current study examines what impact these cultural identity variables, in addition to perceptions of barriers to college entrance, would have on educational aspirations and college-going self-efficacy beliefs of Latino adolescents. Findings from 171 middle- and high school Latino students from immigrant families indicated that public ethnic regard and resilience to barriers were positively associated with college-going self-efficacy, and Anglo orientation had a trend-level effect, while perceived barriers were negatively related to that outcome. Private ethnic regard and person-based barriers were negatively associated with educational aspirations. Generation status, gender, mother’s education, and age were control variables. Implications for research and practice are provided, focusing on perceived barriers and self-efficacy beliefs.
- Published
- 2013
132. Review of ‘Americans by Heart: Undocumented Latino students and the promise of higher education.’
- Author
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
- Abstract
In August 2012, undocumented youth who were brought to the United States prior to their 16th birthday became eligible to apply for deferred action relative to deportation, per an administrative order by President Barack Obama. Although this action did not accomplish the same goals hoped for by Development, Relief, and Education for Alien Minors (DREAM) Act supporters, it did allow a portion of the unauthorized immigrant youth in this country a chance to apply for a temporary work permit and to come out from the shadows. At the start of his second term in January 2013, President Obama and a bipartisan group of Senators went to work drafting legislation for more comprehensive immigration reform. This historical moment makes William Pérez's book Americans by Heart all the more salient. The book is a product of his 2-year qualitative and quantitative study of undocumented Latino high school students (18% of the sample) and college students (34% attending community college, 48% at a 4-year college), mostly in California and Texas. The book illustrates what he learned about their social resources and constraints (Chapter 2), their academic and civic engagement (Chapters 3 and 4), their pathways into higher education (Chapter 5), and their status upon graduation (Chapter 6). Most important, the voices of the participants bring the reasons for deferred action and the DREAM Act vividly to life. In one participant's words, “I was really depressed because I was outstanding in school, and I was like, OK, I can't go to college” (p. 24). The determination and motivation of the participants is shown as well, as they continued to strive and often to excel against long odds.
- Published
- 2013
133. Quality of Interactions in Face-to-Face and Hybrid Career Development Courses: An Exploration of Students' Perceptions
- Author
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
- Abstract
Online counselor education is growing, but there has been little conclusive research about its effectiveness. This paper reports on an exploratory study in which the perceptions of students in a hybrid career development and counseling class were compared to those of students in a face-to-face offering of the same class. Student-to-student and student-to-instructor interactions were examined and several themes were identified: nature of interactions, sufficiency of interactions, different types of communication, impact of interactions on learning, and enhancement of relationships. Both delivery formats were found to have benefits and challenges. The paper concludes with a discussion of the practical implications and areas in need of future research.
- Published
- 2013
134. The Protective Role of Familism in the Lives of Latino Adolescents
- Author
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Supple, Andrew "Andy", Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., and Supple, Andrew "Andy"
- Abstract
Familism, a Latino value that promotes loyalty, cohesiveness, and obedience within the family, predicts improved outcomes for Latino adolescents. However, few studies have tested whether familism serves a protective role when adolescents are facing stress. We examined whether familism predicted psychosocial outcomes in the context of stress, and whether familism moderated the relationship between peer discrimination, acculturative stress, and economic stress predicting these outcomes in a sample of 173 Latino adolescents. Familism was associated with fewer depressive symptoms and greater school attachment, but it did not moderate the relationship between any of the stressors and outcomes. Discrimination was associated with greater depressive symptoms, worse school attachment, and greater perceived barriers to college, but socioeconomic stress and acculturation stress did not uniquely predict these outcomes once taking into account discrimination. Thus, although familial culture values lead to improved outcomes in youth, they are unable to counter the detrimental effects of discrimination.
- Published
- 2013
135. Socioeconomic Stress and Academic Adjustment Among Asian American Adolescents: The Protective Role of Family Obligation
- Author
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Supple, Andrew "Andy", Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., and Supple, Andrew "Andy"
- Abstract
Socioeconomic stress has long been found to place youth at risk, with low family income conferring disadvantages in adolescents’ school achievement and success. This study investigates the role of socioeconomic stress on academic adjustment, and pinpoints family obligation as a possible buffer of negative associations. We examined direct and interactive effects at two time points in the same sample of Asian American adolescents—early high school (N = 180 9th–10th graders; 60 % female) and 2 years later in late high school (N = 156 11th–12th graders; 87 % of original sample). Results suggest that socioeconomic stress is indeed associated with poor academic adjustment, measured broadly through self-reported GPA, importance of academic success, and educational aspirations and expectations. Family obligation was positively related to adjustment, and also was found to buffer the negative effects of socioeconomic stress, but only during adolescents’ later high school years. Adolescents reporting more family obligation experienced less of the negative effects of financial stress on academic outcomes than those reporting lower obligation. Cultural and developmental implications are discussed in light of these direct and moderating effects.
- Published
- 2013
136. Parental involvement in children's education: considerations for school counselors working with Latino immigrant families
- Author
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Borders, L. DiAnne, Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Borders, L. DiAnne, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
- Abstract
The Hoover-Dempsey et al. (2005) model of parents" involvement in their children's schools has recently been applied to Latino immigrant families. This article reviews that literature and then describes culturally appropriate strategies for school counselors to work with this population, focusing on (a) teacher and counselor invitations to the family, (b) parent or partnership-focused role construction, and (c) flexible formats for involvement that respect families with limited time. The article includes a sample Latino Family Night program
- Published
- 2013
137. Gendered academic adjustment among Asian American adolescents in an emerging immigrant community.
- Author
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Supple, Andrew "Andy", Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., and Supple, Andrew "Andy"
- Abstract
Research on the academic adjustment of immigrant adolescents has been predominately conducted in large cities among established migration areas. To broaden the field’s restricted focus, data from 172 (58% female) Asian American adolescents who reside within a non-traditional or emerging immigrant community in the Southeastern US were used to examine gender differences in academic adjustment as well as school, family, and cultural variables as potential mediators of gender differences found. Results suggest that girls report significantly higher educational goals, intrinsic academic motivation, and utility value of school compared to boys. These gender differences are statistically mediated by ethnic exploration and family processes, most prominently, family respect. School connectedness and perceived discrimination are also associated with academic adjustment at the bivariate level, suggesting that academic success may be best promoted if multiple domains of influence can be targeted.
- Published
- 2012
138. Cultural stressors and the hopelessness model of depressive symptoms in Latino adolescents.
- Author
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Gonzalez, Laura McLaughlin, Huq, Nadia, NC DOCKS at The University of North Carolina at Greensboro, Stein, Gabriela L., Gonzalez, Laura McLaughlin, Huq, Nadia, NC DOCKS at The University of North Carolina at Greensboro, and Stein, Gabriela L.
- Abstract
Depressive symptoms in Latino youth have been related to both culturally-universal and culturally-based stressors. However, few studies have examined the unique contributions of culturally-based stressors above and beyond other types of stressors. Moreover, no past studies with Latinos have examined the role of culturally-based stressors within a hopelessness model of depressive symptoms, a cognitive model with the strongest empirical support in adolescence. The current study examined these issues in a sample of 171 Latino adolescents (7th–10th grades; mean age = 14; 46 % male). The Latino adolescents were primarily Mexican–American (78 %) and born in the United States (60 %). Students completed measures during a school period on their experiences of parent–child conflict, economic stress, discrimination from peers, and acculturative stress as well as depressive symptoms and attributional style. The results indicated that culturally-based stressors (e.g., acculturative stress and discrimination) predicted greater depressive symptoms even when controlling for culturally-universal stressors (e.g., parent–child conflict, economic stress). Moreover, a negative attributional style moderated the relationship between culturally-universal stressors and depressive symptoms, but this was not the case for culturally-based stressors. Culturally-based stressors play an important role in depressive symptoms among Latino youth. These stressors predicted greater symptomatology even when controlling for other types of stressors and a negative attributional style. These findings suggest that there may be other cognitive risk factors associated with culturally-based stressors.
- Published
- 2012
139. Latina/o adolescents in an emerging immigrant community: A qualitative exploration of their future goals.
- Author
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Shannonhouse, Laura R., Stein, Gabriela L., Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Shannonhouse, Laura R., and Stein, Gabriela L.
- Abstract
Although immigrant adolescents typically have high hopes for their futures, educational and career outcomes often do not match aspirations. The future aspirations of 17 Latina/o adolescents in an emerging immigrant community were explored. Qualitative interviews were conducted and analyzed using consensual qualitative research methodology (CQR). Interviews focused on goals for education/career and supports and barriers to reaching those goals. Overall, students expressed high aspirations but were unclear on how to achieve them. Family members and school personnel were seen as supportive, but with limitations. Barriers mentioned by most participants included early pregnancy, finances, and circumstances beyond their control; they declined to endorse other barriers when prompted. Students also held less optimistic views of the educational and career possibilities of an “average” Latina/o/a as compared to their own goals, which is framed in terms of stereotypes. A clear theme emerged where students placed the primary responsibility for their success or failure on themselves without acknowledging many barriers in the environment. Findings are discussed from a social justice point of view with implications that pertain to provision of college planning information, context for applying it, affective support, and systemic advocacy.
- Published
- 2012
140. College level choice of Latino high school students: A social cognitive approach.
- Author
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
- Abstract
Latino students attend 2-year colleges more often than 4-year colleges. This has an impact on the rate of bachelor's degree attainment, because the transfer rate between the 2 levels is low. The author uses national data to identify predictors associated with college-level choice and then uses social-cognitive career theory (Lent, Brown, & Hackett, 1994) to frame counseling implications. Los estudiantes Latinos se matriculan con más frecuencia en estudios universitarios de 2 años que en estudios de 4 años. Esto produce un impacto en el porcentaje de obtención de licenciaturas, porque la tasa de transferencia entre ambos niveles es baja. El autor emplea datos a nivel nacional para identificar predictores asociados con la elección del nivel de estudios universitarios, y después emplea la teoría social cognitiva de las carreras (Lent, Brown, & Hackett, 1994) para formular las implicaciones para la consejería.
- Published
- 2012
141. Latino Students in New Arrival States: Factors and Services to Prevent Youth From Dropping Out
- Author
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Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
- Abstract
Latino youth are more likely than any other ethnic group to drop out of high school in the United States. Though some research has helped us understand the factors leading to dropout, very few studies have assessed Latino student’s opinions of services and factors that would help them stay in school (e.g., family, school, peers, and policies). This study presents the results of an in-depth survey of 501 Latino students in North Carolina public schools. Findings suggest that Latino youth drop out because of the difficulty of their school work, personal problems (e.g., pregnancy or problems at home), the need to work to support their family economically, and peer pressure. Students suggest improved academic and personal support in the form of tutoring, mentoring, after-school programs; improved English as a second language classes; and more Spanish-speaking staff/teachers. Recommendations for intervention and policy are suggested.
- Published
- 2010
142. Antioxidants modulate acute solar ultraviolet radiation-induced NF-kappa-B activation in a human keratinocyte cell line
- Author
-
Takashi Okamoto, Toshifumi Tetsuka, Lester Packer, Keiji Iwasaki, Laura McLaughlin, Josiane Cillard, Jian-Ping Yang, Manabu Kitazawa, John K. Lodge, and Claude Saliou
- Subjects
Keratinocytes ,Free Radicals ,Ultraviolet Rays ,Gene Expression ,Radiation-Protective Agents ,Biology ,Biochemistry ,Skin Diseases ,Antioxidants ,Cell Line ,chemistry.chemical_compound ,Physiology (medical) ,Gene expression ,medicine ,Humans ,Skin ,Regulation of gene expression ,Thioctic Acid ,Activator (genetics) ,NF-kappa B ,NF-κB ,NFKB1 ,Molecular biology ,Acetylcysteine ,Transcription Factor AP-1 ,IκBα ,HaCaT ,medicine.anatomical_structure ,chemistry ,Keratinocyte ,Silymarin - Abstract
Exposure of the human skin to ultraviolet radiation (UVR) leads to depletion of cutaneous antioxidants, regulation of gene expression and ultimately to the development of skin diseases. Although exogenous supplementation of antioxidants prevents UVR-induced photooxidative damage, their effects on components of cell signalling pathways leading to gene expression has not been clearly established. In the present study, the effects of the antioxidants alpha-lipoic acid, N-acetyl-L-cysteine (NAC) and the flavonoid extract silymarin were investigated for their ability to modulate the activation of the transcription factors nuclear factor kappa B (NF-kappaB) and activator protein-1 (AP-1) in HaCaT keratinocytes after exposure to a solar UV simulator. The activation of NF-kappaB and AP-1 showed a similar temporal pattern: activation was detected 2 h after UV exposure and maintained for up to 8 h. To determine the capacity of activated NF-kappaB to stimulate transcription, NF-kappaB-dependent gene expression was measured using a reporter gene assay. The effects of the antioxidants on NF-kappaB and AP-1 activation were evaluated 3 h after exposure. While a high concentration of NAC could achieve a complete inhibition, low concentrations of alpha-lipoic acid and silymarin were shown to significantly inhibit NF-kappaB activation. In contrast, AP-1 activation was only partially inhibited by NAC, and not at all by alpha-lipoic acid or silymarin. These results indicate that antioxidants such as alpha-lipoic acid and silymarin can efficiently modulate the cellular response to UVR through their selective action on NF-kappaB activation.
- Published
- 1999
143. Abstract C221: Glycoengineered anti-EGFR (GA201) elicits enhanced ADCC responses by NK cells from colorectal cancer patients despite tumor-associated impairments to natural cytotoxicity
- Author
-
Suzanne Allen, Anton Belousov, Aniekan Etuk, Laura McLaughlin, Pablo Umana, David Oppenheim, Rebecca Prue, Claudia Pena-Murillo, David Malone, Paul Ross, Farzin Farzaneh, Roberto Spreafico, and Timothy Murray
- Subjects
Antibody-dependent cell-mediated cytotoxicity ,Cancer Research ,biology ,Cetuximab ,business.industry ,chemical and pharmacologic phenomena ,CD16 ,Interleukin 21 ,Cell killing ,Immune system ,Immunophenotyping ,Oncology ,Immunology ,biology.protein ,Medicine ,Antibody ,business ,medicine.drug - Abstract
Background: One component of the therapeutic efficacy of IgG1 monoclonal antibodies (mAb) is their ability to induce antibody dependent cellular cytotoxicity (ADCC) by NK cells and other immune effectors bearing the low-affinity Fc RIIIa (CD16) receptor. Clinical studies establish the relevance of ADCC finding widespread polymorphisms that encode a very low affinity CD16 (158F/F) predict limited clinical responses to Cetuximab or Rituximab. Now, mAbs with increased affinity for CD16 can be produced using GlycoMab technology. The altered glycosylation pattern in the Fc domain of these antibodies enhances CD16-binding and ADCC irrespective of genotype. Yet, the potential for enhanced ADCC in patients with advanced colorectal carcinoma (CRC) has not been demonstrated. Considering the potential for tumor-associated immune impairments and treatments-associated leukopenia, we enumerated and characterized NK cells from patients prior to chemotherapy, on active therapy, or following two-lines of standard treatment and compared responsiveness to standard and glycoengineered anti-EGFR mAbs. Methodology: PBMCs were isolated from blood of age-matched healthy donors and 100 patients: either at presentation with metastatic disease; during chemotherapy with FOLFOX, FOLFIRI, or Irinotecan; or at disease progression >4 weeks following second line failure. Laboratory tests included: immunophenotyping for CD3,CD8,CD45,CD4 and CD3,CD56/16,CD45,CD19 markers; measurements of CD16- and NKG2D-expressing NK cells; CD16 158 genotype; and assessment of K562-killing (natural cytotoxicity) and degranulation (CD107+) of NK cells among cryopreserved PBMC in response to K562 or EGFR+ A431 cells with 10 g/ml GlycoMAb anti-EGFR (GA201), wild-type GA201, Panitumumab or Cetuximab. Results: For each group of patients, the proportion of NK cells among lymphocytes remained similar to controls. However, NK cells counts were reduced about half in patients (affected primarily by disease and only slightly by treatment). Per NK cell killing capacity of K562 was substantially impaired in patients prior to therapy and most profoundly among the post-chemotherapy group. ADCC responses were similarly impaired but to lesser degrees. In all cohorts tested, GlycoMAb anti-EGFR was superior to cetuximab in activating more NK cells. The superior ADCC activation of NK cells by GlycoMAb was most pronounced in the patients with chemotherapy-resistant disease. Conclusion: Chemotherapy does not induce gross alterations in the immune phenotype of CRC patients. At all stages of treatment, patients retain CD16+ NK cells capable of ADCC. The impairments in the NK functional responses tested are likely due to host/tumor interaction and not a consequence of treatment. Despite impairments, the best activation of NK cells was always in response to GlycoMAb GA201 for all individuals tested. These findings support testing for enhanced ADCC by GA201 in clinical trials of CRC patients following two lines of chemotherapy or in combination with chemotherapy. Acknowledgements: Simon Hollingsworth, current affiliation Astra Zeneca UK Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2011 Nov 12-16; San Francisco, CA. Philadelphia (PA): AACR; Mol Cancer Ther 2011;10(11 Suppl):Abstract nr C221.
- Published
- 2011
- Full Text
- View/download PDF
144. 160-P Complete genomic characterization of 37 common and well-documented HLA class I alleles using next-generation sequencing technology
- Author
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Deborah Ferriola, Laura McLaughlin, Xiaowu Gai, Ariella Sasson, Curt Lind, and Dimitri S. Monos
- Subjects
Cancer genome sequencing ,Whole genome sequencing ,Single cell sequencing ,Shotgun sequencing ,Immunology ,Immunology and Allergy ,General Medicine ,Computational biology ,Human leukocyte antigen ,Biology ,DNA sequencing ,Exome sequencing ,Deep sequencing - Published
- 2011
- Full Text
- View/download PDF
145. Patching the Leaky Pipeline
- Author
-
Gonzalez, Laura McLaughlin, NC DOCKS at The University of North Carolina at Greensboro, Gonzalez, Laura McLaughlin, and NC DOCKS at The University of North Carolina at Greensboro
- Abstract
The United States faces the critical task of graduating large numbers of individuals in mathematics, science, technical, and engineering fields. The first-year orientation course described uses female-friendly practices to provide support, promote academic success, promote major and career development in an MSTE field, and facilitate consideration of issues faced by women in undergraduate science majors.
- Published
- 2002
146. Self-Evaluation of Interpersonal Relationships Form
- Author
-
Thelen, Mark H., primary, Farmer, Janet, additional, Mann, Laura McLaughlin, additional, and Pruitt, Julie, additional
- Published
- 1990
- Full Text
- View/download PDF
147. Attitudes and Knowledge Regarding Bulimia and Anorexia Nervosa.
- Author
-
Smith, Marcia C., Pruitt, Julie A., Mann, Laura McLaughlin, and Thelen, Mark H.
- Subjects
BULIMIA ,ANOREXIA nervosa ,MENTAL health ,PSYCHOTHERAPY ,COLLEGE student attitudes ,HEALTH attitudes - Abstract
The current study investigated general knowledge of the symptoms, causes, and treatment of bulimia and anorexia nervosa, as well as attitudes toward anorexics and bulimics. High school and college students, 15 to 25 years of age, were administered an anonymous questionnaire. Adequate definitions of bulimia and anorexia were provided by 44% and 71% of the subjects, respectively. Females demonstrated more knowledge than did males. Emotional problems were seen as the most likely cause of both disorders, and psychotherapy was considered the most effective treatment. The subjects were more rejecting of an anorexic female, an obese female, and an average weight vomiter than an average weight binger. Over 41% of the males were rejecting of an anorexic, obese, or purger female as a dating partner. [ABSTRACT FROM AUTHOR]
- Published
- 1986
- Full Text
- View/download PDF
148. Bulimia: Prevalence and component factors in college women
- Author
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Julie Pruitt, Laura McLaughlin Mann, Marcia Smith, and Mark H. Thelen
- Subjects
Adult ,Validity ,Menstruation problems ,behavioral disciplines and activities ,United States ,Developmental psychology ,Manuals as Topic ,Psychiatry and Mental health ,Clinical Psychology ,Cross-Sectional Studies ,Ingested food ,mental disorders ,Vomiting ,medicine ,Humans ,Female ,Bulimia ,medicine.symptom ,Overeating ,Psychology ,Demography - Abstract
Using a measure of bulimia (BULIT) that has demonstrated validity and reliability, the prevalence of bulimia among three samples of college females was found to range from 2.0 to 3.8%. These percentages are substantially lower than most previous reports would suggest. Factor analysis of the BULIT data revealed that the binging factor accounted for 63% of the variance. Other factors identified include vomiting, negative feelings about overeating, menstruation problems, preference for high caloric/easily ingested food, and weight fluctuations. The intercorrelations among the factors were examined and the implications of these data for the proposed revised DSM-III criteria for bulimia are discussed.
- Published
- 1987
- Full Text
- View/download PDF
149. Attitudes and knowledge regarding bulimia and anorexia nervosa
- Author
-
Laura McLaughlin Mann, Mark H. Thelen, Marcia C. Smith, and Julie A. Pruitt
- Subjects
Psychiatry and Mental health ,medicine.medical_specialty ,Anorexia nervosa (differential diagnoses) ,mental disorders ,medicine ,Effective treatment ,General knowledge ,Anorexia ,medicine.symptom ,Body weight ,Psychology ,Psychiatry - Abstract
The current study investigated general knowledge of the symptoms, causes, and treatment of bulimia and anorexia nervosa, as well as attitudes toward anorexics and bulimics. High school and college students, 15 to 25 years of age, were administered an anonymous questionnaire. Adequate definitions of bulimia and anorexia were provided by 44% and 71% of the subjects, respectively. Females demonstrated more knowledge than did males. Emotional problems were seen as the most likely cause of both disorders, and psychotherapy was considered the most effective treatment. The subjects were more rejecting of an anorexic female, an obese female, and an average weight vomiter than an average weight binger. Over 4 7 % of the males were rejecting of an anorexic, obese, or purger female as a dating partner.
- Published
- 1986
- Full Text
- View/download PDF
150. URINARY EXCRETION OF ORGANIC ACID AND ITS VARIATION WITH DIET
- Author
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Katharine Blunt and Laura McLaughlin
- Subjects
chemistry.chemical_classification ,Variation (linguistics) ,Urinary excretion ,Animal science ,chemistry ,Cell Biology ,Molecular Biology ,Biochemistry ,Organic acid - Published
- 1923
- Full Text
- View/download PDF
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