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841 results on '"Kubista E"'

101. [C-erbB-2 oncogene amplification in breast cancer in correlation to steroid and epidermal growth factor receptor]

Author

Manavi M, Kf, Czerwenka, Robert Zeillinger, Speiser P, Kury F, Steinhart U, Peschke M, Kubista E, and Reinold E

Subjects
Receptor, ErbB-2, Gene Amplification, Breast Neoplasms, ErbB Receptors, Immunoenzyme Techniques, Carcinoma, Intraductal, Noninfiltrating, Receptors, Estrogen, Lymphatic Metastasis, Proto-Oncogene Proteins, Biomarkers, Tumor, Humans, Female, Breast, Lymph Nodes, Receptors, Progesterone, Neoplasm Staging
Abstract

The amplification grade of oncogene c-erbB-2 was examined by the polymerase-chain-reaction-method in DNA's of 56 primary mammary carcinomas. 26 (46.4%) of these showed the amplified oncogene c-erbB-2. In the strongly amplified cases, the expression of the c-erbB-2 oncoprotein was verifiable immunohistochemically. Between the progesterone receptor status (PR) and the amplified c-erbB-2 oncogene there was a statistically proven dependency. No correlation was observed between the amplified c-erbB-2 oncogene and the epidermal growth-factor receptor (EGFR).

Published
1992

103. Intratumoral IGF-I protein expression is selectively upregulated in breast cancer patients with BRCA1/2 mutations

Author

Hudelist, G, primary, Wagner, T, additional, Rosner, M, additional, Fink-Retter, A, additional, Gschwantler-Kaulich, D, additional, Czerwenka, K, additional, Kroiss, R, additional, Tea, M, additional, Pischinger, K, additional, Kostler, W J, additional, Attems, J, additional, Mueller, R, additional, Blaukopf, C, additional, Kubista, E, additional, Hengstschlager, M, additional, and Singer, C F, additional

Published
2007
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123. [Use of polymerase chain reaction (PCR) as an additional diagnostic method in gynecologic oncology]

Author

Kf, Czerwenka, Robert Zeillinger, Kury F, Manavi M, Speiser P, Peschke M, Steinhart U, Jw, Hosmann, and Kubista E

Subjects
Neoplasms, Hormone-Dependent, Receptor, ErbB-2, Gene Amplification, Breast Neoplasms, Polymerase Chain Reaction, ErbB Receptors, Gene Expression Regulation, Neoplastic, Carcinoma, Intraductal, Noninfiltrating, Receptors, Estrogen, Proto-Oncogene Proteins, Humans, Female, Neoplasm Invasiveness, Breast, Receptors, Progesterone
Published
1991

127. Insulin-like growth factor -1 (IGF-1) und IGF-1R in BRCA-1/2–Gen verändertem Mammakarzinom: Überexpression von IGF-1 in Mutationsträgerinnen

Author

Hudelist, G, primary, Wagner, T, additional, Fink-Retter, A, additional, Gschwantler-Kaulich, D, additional, Czerwenka, K, additional, Kroiss, R, additional, Muy-Kheng, T, additional, Pischinger, K, additional, Köstler, W, additional, Attems, J, additional, Müller, R, additional, Blaukopf, C, additional, Kubista, E, additional, and Singer, C, additional

Published
2006
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133. Determination of HER-2/neu amplification and expression in tumor tissue and cultured cells using a simple, phenol free method for nucleic acid isolation

Author

Fd, Kury, Schneeberger C, Sliutz G, Kubista E, Salzer H, Medl M, Leodolter S, Swoboda H, Robert Zeillinger, and Spona J

Subjects
Ovarian Neoplasms, Receptor, ErbB-2, Breast Neoplasms, DNA, DNA, Neoplasm, Centrifugation, Isopycnic, Proto-Oncogene Mas, Cell Line, Gene Expression Regulation, Neoplastic, Blotting, Southern, Proto-Oncogene Proteins, Humans, RNA, Female, RNA, Neoplasm, Laryngeal Neoplasms, Nucleic Acid Amplification Techniques
Abstract

A rapid, simple and non-toxic procedure for the simultaneous isolation of DNA and RNA from tumor tissue and cells grown in vitro is described. Guanidinium isothiocyanate was used for homogenization of tumor tissue and for cell lysis. Separation of proteins, DNA and RNA was carried out by isopycnic centrifugation in cesium trifluoroacetate. DNA was further purified by salting out residual protein. Nucleic acids prepared by this method from 47 primary human carcinomas and 17 human cell lines were analysed for amplification and expression of the HER-2/neu proto-oncogene. 2- to 10-fold amplification of HER-2/neu was noted in 7/22 mammary carcinomas (32%) and in 4/14 ovarian carcinomas (28%). No amplification of the proto-oncogene was found in 4 laryngeal carcinomas, 1 pharyngeal carcinoma, 2 retrolingual carcinomas, 3 gastric carcinomas and 1 kidney carcinoma. HER-2/neu overexpression was observed in 6/22 of mammary carcinomas (27%) and 7/14 of ovarian carcinomas (50%). No overexpression was found in all other carcinomas studied. Concordance between amplification and overexpression was noted in 3 mammary and 4 ovarian carcinomas, respectively. 3 mammary and 3 ovarian carcinomas showed overexpression without amplification. 5 human mammary carcinoma cell lines showed both amplification and overexpression of HER-2/neu. In two mammary carcinoma cell lines (MDA MB-453 and ZR 75-1) overexpression was noted without amplification of the proto-oncogene. These data combine to suggest that mechanisms other than gene amplification may also lead to overexpression of the HER-2/neu protooncogene in cancer cells.

Published
1990

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