101. Xanthine oxidase-derived reactive oxygen species contribute to the development of d-galactosamine-induced liver injury in rats.
- Author
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Ohta, Yoshiji, Matsura, Tatsuya, Kitagawa, Akira, Tokunaga, Kenji, and Yamada, Kazuo
- Subjects
XANTHINE oxidase ,REACTIVE oxygen species ,LIVER injuries ,LABORATORY rats ,PEROXIDES ,GLUTATHIONE - Abstract
We examined whether xanthine oxidase (XO)-derived reactive oxygen species (ROS) contribute to the development of d-galactosamine (d-GaIN)-induced liver injury in rats. In rats treated with d-GaIN (500 mg/kg), liver injury appeared 6 h after treatment and developed until 24 h. Hepatic XO and myeloperoxidase activities increased 12 and 6 h, respectively, after d-GalN treatment and continued to increase until 24 h. d-GalN-treated rats had increased hepatic lipid peroxide (LPO) content and decreased hepatic reduced glutathione (GSH) and ascorbic acid contents and superoxide dismutase (SOD), catalase and Se-glutathione peroxidase (Se-GSHpx) activities at 24 h, but not 6 h, after treatment. Allopurinol (10, 25 or 50 mg/kg) administered at 6 h after d-GalN treatment attenuated not only the advanced liver injury and increased hepatic XO activity but also all other changes observed at 24 h after the treatment dose-dependently. These results suggest that XO-derived ROS contribute to the development of d-GaIN-induced liver injury in rats. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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