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The highly selective capture and efficient utilization of recovered Cu(II) ions using a multifunctional material holds substantial practical significance. In this study, a novel type of magnetic adsorbent, MSBA-IIA, was successfully synthesized by grafting a Cu(II) ion-imprinted quaternized salicylaldehyde Schiff base coating onto the surface of magnetic mesoporous silica, aiming to enhance the recognition and separation selectivity for target ions during the adsorption process. Batch Cu(II) ion adsorption experiments demonstrated a dependence of adsorption efficiency on the solution pH level. The Cu(II) adsorption behavior onto MSBA-IIA followed the pseudo-second-order adsorption kinetic model and Langmuir isothermal model. MSBA-IIA exhibited a maximum adsorption capacity of 32.7 mg/g, surpassing that of the non-ion-imprinted counterpart MSBA-NIIA. Thermodynamic study unveiled the spontaneous and exothermic nature of the adsorption process. The relative separation factors α' of MSBA-IIA for Cu(II) were determined to be much more than 1, suggesting that the imprinted adsorbent exhibited superior selectivity for Cu(II) ions in the presence of other co-existing metal ions when compared to the MSBA-NIIA. Due to its magnetic collection and regenerable nature, the proposed approach holds promise for the selective removal of Cu(II) ions from water. Highlight: MSBA-IIA with surface Cu(II) ion-imprinted coating was prepared. MSBA-IIA is capable of selectively adsorbing and recovering Cu(II) ions. The maximum adsorption capacity is 32.7 mg/g for Cu(II) ions. The adsorbent shows excellent reusability. [ABSTRACT FROM AUTHOR]

Escherichia coli (E. coli) O157:H7 is an important food-borne pathogen that can cause hemorrhagic diarrhea and enteritis in humans and animals. Realizing the rapid quantitation of E. coli O157:H7 is of great significance for the guarantee of food safety and disease control. In this study, an electrochemical immunosensing technique based on a functionalized composite of Cu-metal organic framework (Cu-MOF) and poly (3,4-ethylenedioxythiophene)-poly (styrenesulfonate) (PEDOT:PSS) is developed, achieving rapid and sensitive quantitation of E. coli O157:H7 in food and clinical feces samples. The organic functionalization of Cu-MOF significantly improves the interface conductivity to facilitate electron transfer and provides the sulfonic groups (-SO 3 H) to conjugate bio-recognizing elements for target determination. The immunosensor delivers a linear detection range of 3 × 10 2  ~ 3 × 10 8  cfu/mL, a low limit of detection (LOD) of 7.4 cfu/mL, and a short analysis time of 40 min. In addition, it does not show any cross-reactivity with other common pathogens and exhibits high repeatability with relative standard deviations (RSDs) all lower than 2.09%, providing a promising approach for warranting  food safety and control of E. coli O157:H7 disease., Competing Interests: Declarations Ethical approval Not applicable. Conflict of interest The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

Background: Globally, hepatitis B infection (HBI) poses a substantial public health concern and healthcare workers, including nursing students, are at a higher risk of contracting this disease. Thus, the study aimed to assess how knowledge, attitudes, and sociodemographic factors are associated with HBI prevention among a sample of Bangladeshi nursing students. Methods: A cross-sectional survey was performed among 737 nursing students from the nursing institutes of Khulna and Barishal divisions in Bangladesh from January to April 2023. The data were collected by providing questionnaires (structured questionnaire) in the classroom, following a stratified random sampling process. A model of multinomial logistic regression was used to evaluate the factors linked to HBI prevention practices. Result: The mean (SD) scores were 11.42 (± 2.88) for knowledge, 4.33 (± 1.91) for attitude and 4.27 (± 2.056) for practice respectively. Participants' low knowledge (adjusted odds ratio, aOR = 2.562, 95% CI: 1.29–5.07) and poor attitude (aOR = 5.730, 95% CI: 3.19–10.28) regarding HBI were significantly associated with higher likelihood of poor practice towards HBI prevention. Moreover, being 2nd year of nursing students (aOR = 2.147, 95% CI: 1.19–3.86), being aged 19–20 years (aOR = 3.038, 95% CI: 1.30–7.09), being married (aOR = 0.320, 95%CI: 0.13–0.82) and having a family history of HBI (aOR = 0.134, 95%CI: 0.05–0.36) were significantly associated with poor practices of HBI prevention among study participants. Conclusion: The knowledge, attitude and practice scores of the nursing students on HBI prevention were suboptimal. We advocate for implementing regular HBI prevention education and policies, free or subsidized services, skill development, proper HBI prevention enforcement and strict professional ethics within nursing colleges. Such efforts should predominantly focus on second-year, aged 19–20 and unmarried nursing students. [ABSTRACT FROM AUTHOR]

This thorough review examines the latest developments in Metal-Organic Gels (MOGs), including their structure, properties, synthesis, and characterization methods. The scientific community is captivated by MOGs and their unique attributes, sparking interest and envisioning potential applications in various domains. This paper emphasizes the profound influence of synthetic parameters, including solvent choice, pH, concentration, temperature, reaction duration, and metal-to-ligand ratio, on the morphology, mechanical characteristics, kinetics, and overall optimization of MOGs. Understanding the fundamental mechanisms governing MOG formation and appreciating the pivotal role of synthetic conditions in tailoring their properties is imperative. Various synthetic strategies for MOG fabrication and a spectrum of characterization techniques are examined, offering insight into prospective applications. However, looking ahead, there is a clear need for a more organized approach to research, focusing on streamlining synthetic processes and advancing characterization methodologies. This will unlock the full potential of MOGs, propelling innovative and tailored properties for specific applications, thus driving the field towards a promising future. Consequently, this review underscores the urgency for sustained research efforts, envisioning a future where MOGs stand as versatile materials, reshaping the landscape of advanced materials and technologies. [ABSTRACT FROM AUTHOR]

Background: Cholangiocarcinoma (CCA) is a biliary epithelial malignant tumor with an increasing incidence worldwide. Therefore, further understanding of the molecular mechanisms of CCA progression is required to identify new therapeutic targets. Methods: The expression of RPL35A in CCA and para-carcinoma tissues was detected by immunohistochemical staining. IP-MS combined with Co-IP identified downstream proteins regulated by RPL35A. Western blot and Co-IP of CHX or MG-132 treated CCA cells were used to verify the regulation of HSPA8 protein by RPL35A. Cell experiments and subcutaneous tumorigenesis experiments in nude mice were performed to evaluate the effects of RPL35A and HSPA8 on the proliferation, apoptosis, cell cycle, migration of CCA cells and tumor growth in vivo. Results: RPL35A was significantly upregulated in CCA tissues and cells. RPL35A knockdown inhibited the proliferation and migration of HCCC-9810 and HUCCT1 cells, induced apoptosis, and arrested the cell cycle in G1 phase. HSPA8 was a downstream protein of RPL35A and overexpressed in CCA. RPL35A knockdown impaired HSPA8 protein stability and increased HSPA8 protein ubiquitination levels. RPL35A overexpression promoted CCA cell proliferation and migration. HSPA8 knockdown inhibited CCA cell proliferation and migration, and reversed the promoting effect of RPL35A. Furthermore, RPL35A promoted tumor growth in vivo. In contrast, HSPA8 knockdown suppressed tumor growth, while was able to restore the effects of RPL35A overexpression. Conclusion: RPL35A was upregulated in CCA tissues and promoted the progression of CCA by mediating HSPA8 ubiquitination. [ABSTRACT FROM AUTHOR]

Ovarian cancer stands as the foremost cause of mortality among gynaecological diseases globally, characterized by high morbidity and mortality. Pinocembrin, a flavonoid from natural plant sources, exhibits diverse pharmacological properties. Despite its known pharmacological activities, its specific role in ovarian cancer treatment remains scarcely reported, and its precise molecular mechanism remains elusive. This study integrates network pharmacology and molecular docking techniques to explore pinocembrin's potential mechanism in ovarian cancer treatment. The targets of pinocembrin were compiled from the several online databases. Ovarian cancer targets were identified using the GeneCards database, with common target genes determined by data aggregation. Protein-protein interactions were analysed using the STRING platform. Subsequent Gene Ontology functional annotation and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed. Molecular docking assessed the binding affinity between potential targets and active compounds. Finally, target validity was verified through in vitro experiments. We identified 163 potential pinocembrin targets for ovarian cancer treatment. GO and KEGG analyses revealed pinocembrin's involvement in protein kinase activity, protein phosphorylation, protein kinase complexes and cancer pathways in ovarian cancer treatment. Molecular docking demonstrated strong binding affinity between pinocembrin and most potential target active sites. In vitro experiments suggested pinocembrin's potential to induce apoptosis in ovarian cancer cells through the AKT1-mTOR signalling pathway. This study comprehensively elucidates pinocembrin's potential targets and mechanisms against ovarian cancer, aiming to provide promising candidates for developing novel and effective alternative and/or complementary nutritional supplements for the clinical treatment of ovarian cancer., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

In this work, new dilute magnetic semiconductor compositions and broad spectrum photocatalysts based on Gd doped and (Gd, Mn) codoped CuO were synthesized by adapted sol gel method. The analyzed XRD peaks were completely indexed to the crystallographic planes of monoclinic copper oxide (CuO) structure. The effectual substitution of Cu2+-sites by Gd and (Gd, Mn) ions was verified from the expansion of the unit cell and shift of the XRD peaks. The inclusion of Gd and (Gd, Mn) ions move the band gap energy of pure CuO (1.41 eV, λ = 879 nm) to low energy region with measured values of 1.3 eV (λ = 953 nm) and 1.26 eV (λ = 984 nm), respectively. The morphological analysis illustrates that the existence of Gd and Mn ions enhances the reduction of the grains size of CuO with more distribution of fine particles. The room temperature ferromagnetic order was impressively improved after incorporation of (Gd, Mn) ions into the lattice of CuO. Herein, Cu0.96Gd0.02Mn0.02O composition exhibits a high saturation magnetization of 1.21 emu/g while the coercivity and remanence values were 156 Oe and 0.15 emu/g, respectively. On the other hand, Cu0.96Gd0.02Mn0.02O as a photocatalyst has large efficiencies for removal of brilliant green and Congo red under normal solar energy and Xenon photoreactor radiation. The measured photodegradation efficiency of Cu0.96Gd0.02Mn0.02O catalyst was 96% and 91% during 70 min with strong stability for 4 series and high mineralization of both dyes to CO2 and H2O. The obtained results highlight that Cu0.96Gd0.02Mn0.02O composition possesses dual-functions operation linked to spin-electronic devices and photocatalysis applications. Highlights: New compositions composed of Gd-doped and (Gd, Mn) codoped CuO. Cu0.96Gd0.02Mn0.02O composition exhibits high room temperature ferromagnetism. Cu0.96Gd0.02Mn0.02O has a remarkable photodegradation for brilliant green and Congo red. Spin-electronic devices and photocatalysis applications. [ABSTRACT FROM AUTHOR]

This report expresses a sustainable methodology for the in situ synthesis of Ag nanoparticles (NPs) adorned carboxymethyl lignin (CML) functionalized Fe3O4 nanocomposite (Fe3O4@CML/Ag) and its subsequent chemical and biological applications. Structural and physicochemical features of such novel material was evaluated through a number of analytical techniques like FT-IR, FE-SEM, TEM, EDS, XRD, ICP and VSM. The nanomaterial was a competent catalyst towards the N-acylation of diverse amines using acetic anhydride at solvent-free conditions. The material was isolated by magnet and reused for 8 consecutive times. Furthermore, the material was employed in the biological evaluation of anti-oxidant properties by DPPH method as well as the anticancer studies of against HT-29 and Caco-2 colon cell lines following MTT method. The DPPH assay afforded a significant IC50 value that suggested the material to have good anticancer properties. Interestingly, % cell viability of the malignant cell lines was found to decrease dose-dependently over Fe3O4@NaLS/Ag nanocomposite. MTT assay afforded the corresponding IC50 values to be 395.4 μg/ml and 287.3 μg/ml respectively against the two cell lines. The absorbance rate was evaluated at 545 nm, which represented toxicity on normal cell line (CHO) even up to 2000 μg/mL. [ABSTRACT FROM AUTHOR]

The present communication deals with the adsorption of tyramine neurotransmitter over the surface of pristine, Boron (B) and Silicon (Si) doped fullerenes. Density functional theory (DFT) calculations have been used to investigate tyramine adsorption on the surface of fullerenes in terms of stability, shape, work function, electronic characteristics, and density of state spectra. The most favourable adsorption configurations for tyramine have been computed to have adsorption energies of − 1.486, − 30.889, and − 31.166 kcal/mol, respectively whereas for the rest three configurations, it has been computed to be − 0.991, − 6.999, and − 8.796 kcal/mol, respectively. The band gaps for all six configurations are computed to be 2.68, 2.67, 2.06, 2.17, 2.07, and 2.14 eV, respectively. The band gap of pristine, B and Si doped fullerenes shows changes in their band gaps after adsorption of tyramine neurotransmitters. However, the change in band gaps reveals more in B doped fullerene rather than pristine and Si doped fullerenes. The change in band gaps of B and Si doped fullerenes leads a change in the electrical conductivity which helps to detect tyramine. Furthermore, natural bond orbital (NBO) computations demonstrated a net charge transfer of 0.006, 0.394, and 0.257e from tynamine to pristine, B and Si doped fullerenes. [ABSTRACT FROM AUTHOR]

In the present study, we have investigated the effect of aqueous extracts of Capparis spinosa leaves (AECSL), buds (AECSB), and fruits (AECSF) on Pb2+ ions. The carbon paste electrode modified with 10% 2-benzimidazole thiol (CPE-10% BIT) is used to reveal the electrochemical behavior of Pb2+ in the presence of the aqueous extract of Capparis spinosa (AECS). Square wave voltammetry (SWV) and cyclic voltammetry (CV) have been used to characterize the electrochemical properties of CPE-10% BIT, while scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) have been applied to study its surface morphology. The presence of AECSL resulted in the complete disappearance of one of the three characteristic Pb2+ peaks observed at − 0.4 V, demonstrating the plant's strong complexation/adsorption capacity. The efficiency of the extract increases linearly with the volume of AECS in the concentration range from 16.66 to 66.66 ml/l (0.24–0.97 g/l) with a complexing effect of 86.08%, 86.1%, and 72.54% at 83.83 ml/l for AECSL, AECSB, and AECSF, respectively. This capacity is related to the hydroxyl (–OH) and pyrone ions present in the aqueous extract, which bind to the Pb2+ ions present in the electrolyte solution, forming a complex with multiple coordination bonds. The effect of the contact time between AECSL and Pb2+ was studied and it was observed that 24.57% complexation/adsorption was reached after 22 min, then this percentage starts to decrease gradually with time. [ABSTRACT FROM AUTHOR]

In the present work, an investigation was performed based on the genera and species stated in Palmer pollution index to show the extent of organic pollution in the surface water of the Tawi watershed in the Jammu province of the Union Territory of Jammu and Kashmir using algal pollution indices. Sampling was carried out for two seasons, pre-monsoon (PRM) and post-monsoon (POM), at 16 locations distributed over the entire Tawi watershed. The physico-chemical variables like water temperature, pH, electrical conductivity, TDS, total alkalinity, total hardness, DO, BOD, COD, nitrate, and phosphate were analyzed. The seasonal distribution of the pollution-tolerant algal genera and species was recorded and the algal pollution index for both genus (AGP index) and species (ASP index) was also calculated. The concentration of BOD, COD, and nitrate in the sampled river water was found to be higher during the PRM season as compared to the POM season. The lower stretch of the watershed (Jammu Sub-Watershed) falls in class IV–V as per the polluted river stretch priority ranking based on BOD levels as BOD levels are >3 mg/L in the downstream locations during both seasons. A total of 23 algal taxa belonging to 8 families, Chlorophyceae (4 algal genera), Cyanophyceae (2 algal genera), Bacillariophyceae (7 algal genera), Zygnematophyceae (3 algal genera), Trebouxiophyceae (2 algal genera), Ulvophyceae (1 algal genus), Mediophyceae (1 algal genus), and Euglenophyceae (3 algal genera), have been reported in the Tawi watershed. The results of the Palmer indices showed a lack of organic pollution in the upstream, varying pollution levels in the midstream, and partially high to very high organic pollution levels in the downstream of the watershed. Comparative temporal analysis of the distribution of pollution-tolerant algal genera and species showed more organic pollution during PRM. Navicula and Cymbella were found to be the most abundant genera in almost all the stations, whereas Ulothrix, Cocconeis, Anacystis, and Crucigenia were the least recorded genera in the entire watershed. The results will enhance the understanding of the health status of the watershed, and provide database for watershed vulnerability assessment for sustainability and watershed management with spatio-temporal improvement. [ABSTRACT FROM AUTHOR]

Background: Ardisia gigantifolia Stapf. (AGS), a Chinese folk medicine widely grows in the south of China and several studies reported that AGS could inhibit the proliferation of breast cancer, liver cancer, and bladder cancer cell lines. However, little is known about its anti-colorectal cancer (CRC) efficiency. Methods: In the present study, a combination of MTT assay, network pharmacological analysis, bioinformatics, molecular docking, and molecular dynamics simulation study was used to investigate the active ingredients, and targets of AGS against CRC, as well as the potential mechanism. Results: MTT assay showed that three kinds of fractions from AGS, including the n-butanol extract (NBAGS), ethyl acetate fraction (EAAGS), and petroleum ether fraction (PEAGS) significantly inhibited the proliferation of CRC cells, with the IC50 values of 197.24, 264.85, 15.45 µg/mL on HCT116 cells, and 523.6, 323.59, 150.31 µg/mL on SW620 cells, respectively. Eleven active ingredients, including, 11-O-galloylbergenin, 11-O-protocatechuoylbergenin, 11-O-syringylbergenin, ardisiacrispin B, bergenin, epicatechin-3-gallate, gallic acid, quercetin, stigmasterol, stigmasterol-3-o-β-D-glucopyranoside were identified. A total of 173 targets related to the bioactive components and 21,572 targets related to CRC were picked out through database searching. Based on the crossover targets of AGS and CRC, a protein-protein interaction network was built up by the String database, from which it was concluded that the core targets would be SRC, MAPK1, ESR1, HSP90AA1, MAPK8. Besides, GO analysis showed that the numbers of biological process, cellular component, and molecular function of AGS against CRC were 1079, 44, and 132, respectively, and KEGG pathway enrichment indicated that 96 signaling pathways in all would probably be involved in AGS against CRC, among which MAPK signaling pathway, lipid, and atherosclerosis, proteoglycans in cancer, prostate cancer, adherens junction would probably be the major pathways. The docking study verified that AGS had multiple ingredients and multiple targets against CRC. Molecular dynamics (MD) simulation analysis showed that the binding would be stable via forming hydrogen bonds. Conclusion: Our study showed that AGS had good anti-CRC potency with the characteristics of multi-ingredients, -targets, and -signaling pathways. [ABSTRACT FROM AUTHOR]

Heat shock protein 90 (Hsp90) is considered an attractive therapeutic target for cancer treatment due to its high expression in many cancers. In this study, four potent Hsp90 inhibitors (HPs 1–4) were identified using structure-based virtual screening. Among them, HP-4 exhibited the most potent inhibitory effects (IC50 = 17.64 ± 1.45 nM) against the Hsp90 protein, which was about 7.7 times stronger than that of MPC-3100 (a positive inhibitor targeting Hsp90). In vitro cytotoxicity assay suggested that HP-4 could effectively inhibit the proliferation of a series of tumour cells, including HCT-116, HeLa, A549, A2780, DU145, HepG2 and A498. Furthermore, in vivo assay displayed that HP-4 had significant anti-tumour effects on HCT-116 cell-derived xenograft models. These data demonstrate that HP-4 could be a potential lead compound for the further investigation of anti-tumour drugs. [ABSTRACT FROM AUTHOR]

Due to less sensitivity of graphene towards the cisplatin (CPT), the graphene is modified by doping systematically boron atoms (B/Gra), nitrogen atoms (N/Gra), and hexagonal boron nitride (BN/Gra) to form hetero-quantum dots (QDs). In our DFT investigations, the CPT drug adsorbs on the surface of modified QDs within favorable range of adsorption energy. Among them, B/Gra QD shows high adsorption behavior with CPT at about −1.44 eV and −0.6 eV energies in gas and water solvent phases. On the other hand, BN/Gra and N/Gra hetero-QDs interact with CPT at about −0.88 eV and −1.03 eV adsorption energies respectively. During interaction of CPT with B/Gra, CPT loses 0.215 e and 0.129 e charge to the B/Gra in gas and solvent phases. About 26.4% and 14% reductions of Eg have been occurred after the interaction of CPT with B/Gra. Furthermore quantum molecular descriptors also suggest the high sensitivity of the B/Gra QDs with the CPT. Therefore, B/Gra QD can be used as drug carrier for CPT. [ABSTRACT FROM AUTHOR]

Background: Colorectal cancer is a disease of unmet medical need. Although extracellular vesicles (EVs) have been implicated in anti-tumor responses, discrepancies were observed among studies. We analyzed the role of tumor-derived EVs (TEVs) in tumor progression in vivo by focusing on regulatory T (Treg) cells, which play essential roles in tumor development and progression. Methods: A mouse model of colorectal cancer lung metastasis was generated using BALB/c mice by tail vein injection of the BALB/c colon adenocarcinoma cell line Colon-26. TEVs derived from Colon-26 and BALB/c lung squamous cell carcinoma ASB-XIV were retrieved from the culture media supernatants. A TEV equivalent to 10 µg protein was injected every other day for 2 weeks. Results: Histology and immunohistochemistry studies revealed that lung tumors reduced in the Colon-26-EV group when compared to the phosphate-buffered saline (PBS) group. The population of CD4 + FoxP3 + cells in the lung was upregulated in the PBS group mice when compared to the healthy mice (P < 0.001), but was significantly downregulated in the Colon-26-EV group mice when compared to the PBS group mice (P < 0.01). Programmed cell death protein 1, glucocorticoid-induced TNFR-related protein, and CD69 expression in lung Treg cells were markedly upregulated in the PBS group when compared to the healthy mice, but downregulated in the Colon-26-EV group when compared to the PBS group. The changes in expression were dose-dependent for Colon-26-EVs. ASB-EVs also led to significantly downregulated Treg cell expression, although non-cancer line 3T3-derived EVs did not. Conclusion: Our study suggests that TEVs possess components for tumor suppression. [ABSTRACT FROM AUTHOR]

Background: The mammalian oviduct is a complex, fibromuscular organ known for its role in orchestrating a series of timely and dynamic changes to suitably support early embryogenesis. Climate change-induced heat stress (HS) is one of the largest single stressors compromising reproductive function in humans and farm animals via systemic changes in the redox status of the maternal environment, adversely affecting fertilization and early embryonic development. Oviductal organoids represent a unique 3-dimensional, biomimetic model to study the physiology of the oviduct and its subsequent impact on embryo development under various environmental conditions. Results: Our study is the first to demonstrate an innovative approach to understanding the cascade of molecular changes sustained by bovine oviductal organoids under HS and the subsequent maternal signals harnessed within their secreted extracellular vesicles (EVs). Transcriptomic analysis of oviductal organoids exposed to HS revealed 2,570 differentially expressed genes (1,222 up‐ and 1,348 downregulated), while EV-coupled miRNome analysis disclosed 18 miRNAs with significant differential expression (12 up- and 6 downregulated) in EVs from thermally stressed organoids compared to EVs released from organoids cultured under thermoneutral conditions. Genes activated in oviductal organoids in response to thermal stress, include: COX1, ACTB, CST6, TPT1, and HSPB1, while miR-1246, miR-148a, miR21-5p, miR-451, and miR-92a represent the top highly abundant EV-coupled miRNAs released in response to HS. Pathway analysis of genes enriched in organoids exposed to thermal stress showed the enrichment of endocrine resistance, cellular senescence, and notch signaling pathways. Similarly, EV-coupled miRNAs released from thermally stressed organoids showed their potential regulation of genes involved in cellular senescence, p53 signaling, and TGF-beta signaling pathways. Conclusions: In conclusion, the cellular and extracellular response of bovine oviductal organoids to in vitro HS conditions reveal the prospective impact of environmental HS on the physiology of the oviduct and the probable subsequent impacts on oocyte fertilization and early embryo development. Future studies elucidating the potential impact of HS-associated EVs from oviductal organoids on oocyte fertilization and preimplantation embryo development, would justify the use of an organoid model to optimally understand the oviduct-embryo communication under suboptimal environments. [ABSTRACT FROM AUTHOR]

In recent years, water pollution has become one of the most dangerous problems facing the world. Pollution of water with heavy metals and different dyes has caused many harmful effects on human health, living organisms and our environment. In this study, iron oxide nanomagnetic composite from Pterocladia Capillacea red algae-derived activated carbon (PCAC-IO) was synthesized by co-precipitation method using different iron salts and different base solutions. The synthesized nanocomposite was investigated with various characterization techniques such as FTIR, BET, SEM-EDX, TEM, XRD, and VSM. The obtained PCAC-IO adsorbent was used for Cr6+ ions and Mordant Violet 40 (MV40) dye removal. The adsorption mechanism of Cr6+ ions and MV40 dye on PCAC-IO was examined using several adsorption and kinetic isotherm models. Langmuir and Freundlich models were investigated using experimental data. Pseudo-first-order (PFO), Pseudo-second-order (PSO) and intraparticle diffusion models (IPDM) were applied to identify the adsorption mechanism. It has shown that the PSO kinetic model fits better with the experimental data obtained from PCAC-IO. This result can be interpreted as the adsorption of the adsorbate on the nanocomposite as chemical adsorption. The optimum conditions for maximum Cr6+ ions removal (96.88%) with PCAC-IO adsorbent occur at room temperature, 5 g L−1 adsorbent concentration, 100 mg L−1 initial pollutant concentration, pH 1 and at the end of 180 min, while maximum MV40 dye removal (99.76%), other conditions being the same, unlikely it occurred at pH 2.06 and after 45 min. The most suitable model for Cr6+ ions removal under the conditions of 1 L−1 g adsorbent concentration and 400 mg L−1 adsorbate concentration was Langmuir (Qmax = 151.52 mg g−1), while for MV40 removal it was Freundlich (Qmax = 303.03 mg g−1). We propose the use of activated carbon-supported iron oxide prepared from bio-waste material, especially from Pterocladia Capillacea red algae, as a promising adsorbent with high efficiency in the removal of Cr6+ ions and MV40 dye from aqueous media. [ABSTRACT FROM AUTHOR]

The environmental-friendly hydrothermal method has been carried out to synthesize Bi2WO6 and g-C3N4/PVP doped Bi2WO6 nanorods (NRs) by incorporating different concentrations of graphitic carbon nitride (g-C3N4) as well as a specified quantity of polyvinylpyrrolidone (PVP). Bi2WO6 doped with g-C3N4 provides structural and chemical stability, reduces charge carriers, degrades dyes, and, owing to lower bandgap energy, is effective for antibacterial, catalytic activity, and molecular docking analysis. The purpose of this research is the treatment of polluted water and to investigate the bactericidal behavior of a ternary system. The catalytic degradation was performed to remove the harmful rhodamine B (RhB) dye using NaBH4 in conjunction with prepared NRs. The specimen compound demonstrated antibacterial activity against Escherichia coli (E. coli) at both high and low concentrations. Higher doped specimens of g-C3N4/PVP-doped Bi2WO6 exhibited a significant improvement in efficient bactericidal potential against E. coli (4.55 mm inhibition zone). In silico experiments were carried out on enoyl-[acylcarrier-protein] reductase (FabI) and β-lactamase enzyme for E. coli to assess the potential of Bi2WO6, PVP doped Bi2WO6, and g-C3N4/PVP-doped Bi2WO6 NRs as their inhibitors and to justify their possible mechanism of action. [ABSTRACT FROM AUTHOR]

The soil microbiota exhibits an important function in the ecosystem, and its response to climate change is of paramount importance for sustainable agroecosystems. The macronutrients, micronutrients, and additional constituents vital for the growth of plants are cycled biogeochemically under the regulation of the soil microbiome. Identifying and forecasting the effect of climate change on soil microbiomes and ecosystem services is the need of the hour to address one of the biggest global challenges of the present time. The impact of climate change on the structure and function of the soil microbiota is a major concern, explained by one or more sustainability factors around resilience, reluctance, and rework. However, the past research has revealed that microbial interventions have the potential to regenerate soils and improve crop resilience to climate change factors. The methods used therein include using soil microbes' innate capacity for carbon sequestration, rhizomediation, bio-fertilization, enzyme-mediated breakdown, phyto-stimulation, biocontrol of plant pathogens, antibiosis, inducing the antioxidative defense pathways, induced systemic resistance response (ISR), and releasing volatile organic compounds (VOCs) in the host plant. Microbial phytohormones have a major role in altering root shape in response to exposure to drought, salt, severe temperatures, and heavy metal toxicity and also have an impact on the metabolism of endogenous growth regulators in plant tissue. However, shelf life due to the short lifespan and storage time of microbial formulations is still a major challenge, and efforts should be made to evaluate their effectiveness in crop growth based on climate change. This review focuses on the influence of climate change on soil physico-chemical status, climate change adaptation by the soil microbiome, and its future implications., (© 2024. Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.)

Nitrite ions (NO 2 - ), as one of the leading type-A inorganic-anion, showing significant-effects in the aquatic environment and also to humans health. Whereas, the higher uptake causes detrimental threat to human health leading to various chronic diseases, thus demanding efficient, reliable and convenient method for its monitoring. For this purpose, in the present research study we have fabricated the mimetic nonozyme like catalyst based colorimetric nitrite sensor. The acetic acid capped Zinc Oxide (ZnO) nanosheets (NSs) were introduce as per-oxidase mimetic like catalyst which shows high efficiency towards the oxidative catalysis of colorless tetramethylbenzidine (TMB) to oxidized-TMB (blue color) in the presence of Hydrogen-peroxide (H 2 O 2 ). The present nitrite ions will stimulate the as formed oxidized-TMB (TMB ox ), and will caused diazotization reaction (diazotized-TMB ox ), which will not only decreases the peak intensity of UV-visible peak of TMB ox at 652 nm but will also produces another peak at 446 nm called as diazotized-TMB ox peak, proving the catalytic reaction between the nitrite ions and TMB ox . Further, the prepared colorimetric sensor exhibits better sensitivity with a wider range of concentration (1 × 10 -3 -4.50 × 10 -1 µM), lowest limit of detection (LOD) of 0.22 ± 0.05 nM and small limit of quantification (LOQ) 0.78 ± 0.05 nM having R 2 value of 0.998. Further, the colorimetric sensor also manifest strong selectivity towards NO 2 - as compared to other interference in drinking water system. Resultantly, the prepared sensor with outstanding repeatability, stability, reproducibility, re-usability and its practicability in real water samples also exploit its diverse applications in food safety supervision and environmental monitoring., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

In this paper, undoped and copper-doped ZnO nanoparticles (NPs) were successfully synthesized using a gliding arc discharge (GAD) plasma technique, which is a sustainable, cost-effective, and scalable method. This method offers several advantages over traditional synthesis methods. The synthesized NPs were characterized by various techniques to understand their physicochemical properties. XRD analysis confirmed the presence of characteristic peaks of pure ZnO, while doped samples exhibited additional peaks corresponding to CuO crystal planes, indicating the successful incorporation of Cu into the lattice. As obvious, bare ZnO showed absorption peak at 378 nm corresponding to the band gap of 3.21 eV. The band gap of Cu-doped samples increased systematically, i.e., 3.35 eV for 2% Cu, 3.47 eV for 4% Cu, and 3.66 eV for 6% Cu. SEM images revealed aggregation and increase in particle size with the increasing in Cu concentration. EDAX analysis revealed a decrease in the weight percentage of oxygen and zinc with the increase in Cu concentration, suggesting structural changes within the lattice. Furthermore, the antibacterial activity against Gram-positive and Gram-negative bacteria, antioxidant activity, and photocatalytic activity against three different organic dyes such as Brilliant Cresyl Blue (BCB), Methylene Blue (MB), and Congo Red (CR) was studied. It is found that the photocatalytic activity of ZnO NPs varies with Cu concentration, leading to a decrease in its performance. The antibacterial activity of the NPs was also assessed, with undoped ZnO NPs showing dose-dependent effects against bacteria, while the Cu-doped ZnO NPs exhibited decreased efficacy. Interestingly, Cu doping significantly enhanced the antioxidant activity of the NPs compared to the undoped ZnO., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The removal of organic dyes has attracted attention by adsorption-photocatalytic synergetic process in water treatment technology. Three novel ZnCo-LDHs/g-C3N4 were successfully prepared for the first time by layered construction technique through the hydrolysis of triethanolamine in this paper. They exhibited high specific surface area which facilitates the adsorption of sunset yellow (SY) from solution to catalyst surface. All the target pollutant dyes are very effectively removed by the three ZnCo-LDHs/g-C3N4 composites through synergetic effect of adsorption and photocatalysis process under UV irradiation (λ = 365 nm). The order of synergistic degradation effect for SY is as follows: ZnCo-LDHs/g-C3N4-3 (99.6%) > ZnCo-LDHs/g-C3N4-2 (99.5%) > ZnCo-LDHs/g-C3N4-1 (99.3%) > pure g-C3N4 (77.4%) > pure ZnCo-LDHs (44.2.6%) at the initial concentration of 75 mg L−1. ZnCo-LDHs/g-C3N4-3 has the largest k value (0.0284 min−1) in SY degradation, which is 2.8 times that of g-C3N4. ZnCo-LDHs/g-C3N4-3 is a very promising adsorption-photocatalyst for the removal of SY from wastewater. The electron spin resonance experiments demonstrate that OH·, 1O2, and O2− are the dominant active species and oxides SY together. This result demonstrates that the three ZnCo-LDHs/g-C3N4 have practical applications as efficient adsorption-photocatalytic materials and also provides a synergetic strategy for the removal of SY wastewater. [ABSTRACT FROM AUTHOR]

Background: Rice husk is the most silicon-rich renewable biomass and can be used as a raw material for producing silicon-based materials. With the increasing interest in utilizing rice husk as a renewable resource, the development of a robust silica extraction method is required. In this study, a one-pot alkali hydrothermal and ball-milling continuous silica extraction method was developed at the pilot scale. Results: Three residence times (50, 100, and 150 min) were selected to compare the performance of the continuous extraction process depending on the residence time. The silica production was 4.09, 2.67, and 2.22 kg day−1 while the silica extraction yield was 52.8, 69.1, and 86.0% at resident times of 50, 100, and 150 min, respectively. The energy consumption for producing 1 kg of silica from rice husk was 51.6, 47.7, and 47.4 MJ kg−1 at resident times of 50, 100, and 150 min, respectively. The one-pot continuous silica extraction process was performed for 150 h to verify the stability of the process. During long-term operation, the process exhibited a constant solid content and stable silica extraction yield. The silicate solution obtained by the one-pot continuous process was successfully used to synthesize size-controlled spherical silica particles, which had a purity of 99.1 wt% and amorphous structure. Conclusions: Overall, this study presents a novel continuous silica extraction method for the efficient recovery of silica from rice husk-based biorefineries. [ABSTRACT FROM AUTHOR]

Here, we propose a green and sustainable 3D porous aerogel based on citrus peel (CP), chitosan (CS), and bentonite (BT). This aerogel is prepared through a simple sol–gel and freeze-drying process and is designed for efficient capture of Cu(II) ions from water matrices. CCBA-2, with its abundance of active binding sites, exhibits an impressive Cu(II) adsorption yield of 861.58 mg/g. The adsorption isotherm and kinetics follow the Freundlich and pseudo-second-order models, respectively. In the presence of coexisting mixed-metal ions, CCBA-2 demonstrates a significantly higher selectivity coefficient (KdCu = 1138.5) for removing Cu(II) ions compared to other toxic metal ions. Furthermore, the adsorption of Cu(II) ions by CCBA-2 is not significantly affected by coexisting cations/anions, ionic strength, organic matter, or different water matrices. Dynamic fixed-bed column experiments show that the adsorption capacity of Cu(II) ions reaches 377.4 mg/g, and the Yoon-Nelson model accurately describes the adsorption process and breakthrough curve. Through experiments, FTIR, and XPS analyses, we propose a reasonable binding mechanism between CCBA-2 and metal cations, involving electrostatic attraction and chemical chelation between Cu(II) and the functional groups of the aerogel. CCBA-2 saturated with Cu(II) ions can be successfully regenerated by elution with 1 M HNO3, with only a slight decrease in adsorption efficiency (5.3%) after 5 adsorption–desorption cycles. Therefore, CCBA-2 offers a cost-effective and environmentally friendly material that can be considered as a viable alternative for the green and efficient removal of toxic Cu(II) ions from wastewater. [ABSTRACT FROM AUTHOR]

The surplus fluoride in drinking water leads to diseases namely fluorosis (dental, skeletal, and non‐skeletal). Hence, to overcome these problems, in this study, hydrotalcite (HT) integrated La‐based metal‐organic frameworks (LaMOFs) namely HT‐LaMOFs was fabricated for fluoride removal. The fabricated HT‐LaMOFs material was well characterized by various characterization methods (x‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), Energy‐dispersive X‐ray spectroscopy (EDAX), thermogravimetric analysis (TGA), and differential thermal analysis (DTA)) to find the structural properties and stability of HT‐LaMOFs. To find the fluoride adsorption nature, the batch studies were employed towards fluoride adsorption such as reaction time, co‐ions, HT‐LaMOFs dosage, pH, initial fluoride concentration, selectivity, pHzpc, and temperature studies. The contact time studies results reveals that the defluoridation capacity (DC) of HT‐LaMOFs was towards fluoride was found as 4451 mgF− kg−1 at 30 min than HT (1030 mgF− kg−1 at 50 min). The DC had little influences in the presence of HCO3− ion compared to other ions. Fluoride adsorption mechanism of HT‐LaMOFs was initiated to be electrostatic interaction, ion‐exchange and complexation. Fluoride adsorption onto HT‐LaMOFs was well matched and followed with Langmuir model. Thermodynamic investigations exposed that the adsorption of fluoride onto HT‐LaMOFs followed by spontaneous and endothermic nature. Kinetic studies of HT‐LaMOFs on fluoride capture followed with pseudo‐second‐order and intraparticle diffusion models. Defluoridation by HT‐LaMOFs was followed endothermic with spontaneous nature of adsorption system. The regeneration study results of HT‐LaMOFs revealed that the developed HT‐LaMOFs was regenerable up to six cycles. In addition, field study outcomes revealed that the developed HT‐LaMOFs diminished fluoride concentration under the acceptable limit as 1.5 mg/L. [ABSTRACT FROM AUTHOR]

Water quality has recently emerged as one of the utmost severe ecological problems being faced by the developing countries all over the world, and Bangladesh is no exception. Both surface and groundwater sources contain different contaminants, which lead to numerous deaths due to water-borne diseases, particularly among children. This study presents one of the most comprehensive reviews on the current status of water quality in Bangladesh with a special emphasis on both conventional pollutants and emerging contaminants. Data show that urban rivers in Bangladesh are in a critical condition, especially Korotoa, Teesta, Rupsha, Pashur, and Padma. The Buriganga River and few locations in the Turag, Balu, Sitalakhya, and Karnaphuli rivers have dissolvable oxygen (DO) levels of almost zero. Many waterways contain traces of NO3, NO2, and PO4−3 pollutants. The majority of the rivers in Bangladesh also have Zn, Cu, Fe, Pb, Cd, Ni, Mn, As, and Cr concentrations that exceed the WHO permissible limits for safe drinking water, while their metal concentrations exceed the safety threshold for irrigation. Mercury poses the greatest hazard with 90.91% of the samples falling into the highest risk category. Mercury is followed by zinc 57.53% and copper 29.16% in terms of the dangers they pose to public health and the ecosystem. Results show that a considerable percentage of the population is at risk, being exposed to contaminated water. Despite hundreds of cryptosporidiosis cases reported, fecal contamination, i.e., Cryptosporidium, is totally ignored and need serious considerations to be regularly monitored in source water. [ABSTRACT FROM AUTHOR]

Background: Overexpression of heat shock proteins (HSPs) has been observed in a wide range of human tumors, and there is an increasing evidence demonstrated that HSPs play a key role in tumor progression. Several studies were conducted to explore the clinicopathological characteristics and prognostic value of HSPs in hepatocellular carcinoma (HCC), but the results remain controversial. To address this gap, we conducted a systematic review and meta-analysis. Methods: The eligible literature was obtained from PubMed, Cochrane library, Web of science, Embase, Chinese National Knowledge Infrastructure and Wan Fang databases. We used the odds ratio (OR) and hazard ratio (HR) as the suitable parameters to assess the clinicopathological features and prognostic value of HSPs in HCC patients. Results: The meta-analysis results showed that HSPs expression was associated with overall survival (OS) of HCCpatients (HR=1.61, 95%CI=1.22-2.13, P=0.001, I²=62.7%). In addition, the pooled results suggested that HSPs expression was significantly correlated with tumor differentiation (OR = 1.33, 95%CI = 1.08-1.65, P = 0.907), vascular invasion (OR = 1.31, 95%CI = 1.02-1.69, P = 0.921) and lymphatic metastasis (OR=1.98, 95%CI= 1.70-2.31, P = 0.740). Meanwhile, the subgroup analysis showed a significant correlation between the expression of HSP27 (HR=1.69, 95%CI = 1.24-2.31, P = 0.674) and HSP90a (HR=2.03, 95%CI = 1.73-2.40, P = 0.743) with OS of HCC patients. Conclusions: Our meta-analysis confirms that HSPs expression is closely associated with a worse prognosis in HCC patients, and may be directly involved in tumor differentiation and distant metastasis. In addition, the subgroup analysis results demonstrate that the expression of HSP27 and HSP90a can be served as potential prognostic predictors of HCC. [ABSTRACT FROM AUTHOR]

Purpose: Dysregulated expression of heat shock proteins (HSP) plays a fundamental role in tumor development and progression. Consequently, HSP90 may be an effective tumor target in oncology, including the treatment of gastrointestinal cancers. Methods: We carried out a systematic review of data extracted from clinicaltrials.gov and pubmed.gov, which included all studies available until January 1st, 2022. The published data was evaluated using primary and secondary endpoints, particularly with focus on overall survival, progression-free survival, and rate of stable disease. Results: Twenty trials used HSP90 inhibitors in GI cancers, ranging from phase I to III clinical trials. Most studies assessed HSP90 inhibitors as a second line treatment. Seventeen of the 20 studies were performed prior to 2015 and only few studies have results pending. Several studies were terminated prematurely, due to insufficient efficacy or toxicity. Thus far, the data suggests that HSP90 inhibitor NVP-AUY922 might improve outcome for colorectal cancer and gastrointestinal stromal tumors. Conclusion: It currently remains unclear which subgroup of patients might benefit from HSP90 inhibitors and at what time point these inhibitors may be beneficial. There are only few new or ongoing studies initiated during the last decade. [ABSTRACT FROM AUTHOR]

Coral islands like Saint Martin’s have long been recognized as being particularly vulnerable to the consequences of climate change. Based on this hypothesis, the broad aim of this research work was to explore the environmental condition of Saint Martin's Island through a geographical illustration of the present status of the landform, and land use and land cover (LULC) characteristics. Both primary and secondary data were used to conduct the research work. Different types of landforms of the island with concerned land use characteristics have been explored through direct field observation. The collected data were analyzed in ArcGIS. Field-level elevation data were collected with global positioning system (GPS) during the field visit, which was used for ground truthing. Secondary data sources include the tidal fluctuation data from the BNHOC and satellite images from Google Earth Pro. The research found rapid changes in the LULC with a sharp increase in built − up areas, and the average tidal fluctuation station value increased by 0.30 metre from 1977 to 2018. This research also found that the total terrestrial area is decreasing due to sea − level rise (SLR). The landform over 3.50 metres is relatively safer from exposure to tidal fluctuation. This study concludes with recommendations to assess and identify the inundation level due to SLR and tidal fluctuation for formulating an effective coastal environment conservation policy. [ABSTRACT FROM AUTHOR]

Nowadays, clean-up of heavy metals from wastewaters using waste residue carbon-based material has received increasing attention. In this work, a novel Chinese medicine residue carbon-based nano zero-valent iron composite (CM-nZVI) had been successfully prepared using the combined Chinese medicine residue, FeCl3 and green tea extract. Cr(VI) and/ or Cd(II) removal in water by the CM-nZVI were systematacially investigated with a series of batch experiments. The most relevant findings indicated the adsorption efficiecy and capacity of Cr(VI) by CM-nZVI were respecitvely nearly 98% and 26 mg/g under optimized reaction conditions. The negative influences of the cations on the Cr(VI) removal followed the order of Al3+ > Ca2+ > Mg2+ Na+ > K+, but the anions followed the order of HCO3− > PO43− > NO3− > Cl− > SO42−. Humic acid (HA) and ionic strength with high concentrations severely inhibited Cr(VI) removal. The Cr(VI) adsorption on CM-nZVI fitted well by the pseudo-second-order kinetic and Langmuir models. A monolayer endothermic chemisorption occurred on Cr(VI) adsorption over CM-nZVI, and Cr(VI) removal by CM-nZVI primarily involved in the absorption, reduction, precipitation and complexation processes. Both Cr(VI) and Cd(II) removals had been achieved by CM-nZVI at their low concentrations. This CM-nZVI showed a better reusability proprity for Cr(VI) and Cd(II) removal with the regeneration of CM-nZVI through simple pickling. The outcomes of this work show that CM-nZVI could be used an effective material for heavy metals removal from water. [ABSTRACT FROM AUTHOR]

Bio-adsorbents derived from Sesbania grandiflora (SGSP/SGSAC) and Amorphophallus paeoniifolius (APSP/APSAC) plants are investigated for their ability to remove nitrite from polluted water. Different extraction conditions have been investigated and optimized for the maximum nitrite removal. Adsorption capacities are found to be 44.5 mg/g for SGSP, 57.5 mg/g for SGSAC, 40.0 mg/g for APSP and 65.5 mg/g for APSAC. Thermodynamic parameters are evaluated. High values of ΔH° for SGSAC (50.872 kJ/mole) and APSAC (67.907 kJ/mole) indicate ion exchange mode of sorption. Spontaneous and endothermic nature of sorption is indicated by negative ΔG values. Positive values of ΔS emphasizes the disorder at solid/liquid interface. The adsorption follows Langmuir isotherm for all the four sorbents, indicating formation of monolayer of nitrite 'onto' the surface of sorbents and uniform distribution active sites. Pseudo second-order kinetics explains well the kinetics of adsorption and this reflects that the adsorption is through exchange of electrons or sharing of electrons between nitrite and functional groups sorbents. The developed sorbents are applied effectively for nitrite removal from polluted lake real water samples. The merit of the present investigation is that simple, effective and easily available plant-based bio-materials are identified for the removal of toxic nitrites from water. [ABSTRACT FROM AUTHOR]

Due to the rarity of gold as a noble metal, efficient and selective Au (III) ions adsorption is critical for gold recovery. Since gold is thiophilic, in this paper, a new covalent thiourea organic polymer (CTOP) was prepared by introducing thiourea reactive groups into the covalent organic polymer of enaminone and applied to the adsorption of Au (III) ions in aqueous solution. The synthesis of the new material was confirmed by SEM, TEM, XRD, and XPS tests. Thanks to the abundant thiourea chelating sites in the CTOP, the total adsorption capacity of the material for Au (III) ions is up to 2372.7 mg/g. In addition, in mixed ionic solutions containing Au (III), Cu (II), Co (II), Cd (II), and Ni (II), it has strong selective adsorption performance for Au (III). It is worth mentioning that the CTOP material still maintains high adsorption performance after five adsorption cycles, which proves that the adsorption material has good reusability. The adsorption kinetics were investigated using pseudo-first-order and pseudo-second-order kinetic models, and the results showed that the adsorption was chemisorption. According to the experimental results of adsorption kinetics and thermodynamics, the adsorption process of CTOP for Au (III) ions conforms to the quasi-secondary adsorption model and the Langmuir adsorption model. We believe that the study of efficient adsorption of Au (III) ions by this new covalent thiourea organic polymer is of great significance for the development of gold recovery technology. [ABSTRACT FROM AUTHOR]

In recent years, nanomaterials have attracted the research intervention of experts in the fields of catalysis, energy, biomedical testing, and biomedicine with their unrivaled optical, chemical, and biological properties. From basic metal and oxide nanoparticles to complex quantum dots and MOFs, the stable preparation of various nanomaterials has always been a struggle for researchers. Microfluidics, as a paradigm of microscale control, is a remarkable platform for online stable synthesis of nanomaterials with efficient mass and heat transfer in microreactors, flexible blending of reactants, and precise control of reaction conditions. We describe the process of microfluidic preparation of nanoparticles in the last 5 years in terms of microfluidic techniques and the methods of microfluidic manipulation of fluids. Then, the ability of microfluidics to prepare different nanomaterials, such as metals, oxides, quantum dots, and biopolymer nanoparticles, is presented. The effective synthesis of some nanomaterials with complex structures and the cases of nanomaterials prepared by microfluidics under extreme conditions (high temperature and pressure), the compatibility of microfluidics as a superior platform for the preparation of nanoparticles is demonstrated. Microfluidics has a potent integration capability to combine nanoparticle synthesis with real-time monitoring and online detection, which significantly improves the quality and production efficiency of nanoparticles, and also provides a high-quality ultra-clean platform for some bioassays. [ABSTRACT FROM AUTHOR]

Rates for the R leads to T conformational change of deoxyhemoglobin formed by laser photolysis of carboxyhemoglobin were determined from CO rebinding observed in three solution systems with viscosities between 1 and 6 cP. Experiments were carried out at 20 degrees C and pH 8.3 in solutions consisting of borate buffer containing various amounts of sucrose, glycerol, or ethylene glycol. As in the case of earlier experiments in borate buffer (Sawicki and Gibson, 1976, J. Biol. Chem., 251:1533-1542), a simple two-state allosteric model which takes into account tetramer-dimer dissociation was found to give a good description of all experimental results. Using measured values for the R- and T-state CO-binding rate constants and the tetramer-dimer dissociation constant, values for the conformational change rate were determined by fitting this model to the experimental data. These rates were compared with Gavish's transient strain model (Gavish, 1978, Biophys. Struct. Mech., 4:37-52), which predicts an inverse dependence of conformational change rate on viscosity. Although fair agreement is found for hemoglobin in sucrose/borate solutions, in glycerol/borate and ethylene glycol/borate solutions, conformational change rate falls off much more rapidly with increasing viscosity than predicted by the model.

A new method for determination of the tetramer-dimer dissociation constant Ku4.2 of deoxyhemoglobin is described. The method involves photolysis of hemoglobin solutions containing a few percent of bound CO (e.g. less than 3%). Under these conditions the nature of the observed CO rebinding is primarily determined by the properties of the dominant species, deoxyhemoglobin. The method makes use of the 30-fold difference in the rate constant describing CO binding to hemoglobin dimers and deoxyhemoglobin tetramers. Because of this large difference in rate constants CO rebinding is made significantly more rapid by the presence of even small concentrations of dimers. Treating this reaction as CO binding to a mixture of hemoglobin dimers and tetramers allows the determination of Ku4.2. Data is presented showing application of the method to human deoxyhemoglobin in the range from pH 9.5 to 11.2.

Cancer is the second leading cause of death in the world and its incidence is expected to increase with the aging of the world's population and globalization of risk factors. Natural products and their derivatives have provided a significant number of approved anticancer drugs and the development of robust and selective screening assays for the identification of lead anticancer natural products are essential in the challenge of developing personalized targeted therapies tailored to the genetic and molecular characteristics of tumors. To this end, a ligand fishing assay is a remarkable tool to rapidly and rigorously screen complex matrices, such as plant extracts, for the isolation and identification of specific ligands that bind to relevant pharmacological targets. In this paper, we review the application of ligand fishing with cancer‐related targets to screen natural product extracts for the isolation and identification of selective ligands. We provide critical analysis of the system configurations, targets, and key phytochemical classes related to the field of anticancer research. Based on the data collected, ligand fishing emerges as a robust and powerful screening system for the rapid discovery of new anticancer drugs from natural resources. It is currently an underexplored strategy according to its considerable potential. [ABSTRACT FROM AUTHOR]

In this study, a novel N-rich triazine-based porous organic polymer (NR-POP) was synthesized via Schiff-base condensation. The structure of the synthesized porous polymer was identified using FT-IR, XRD, SEM, EDS, TEM, TGA, and BET analyses. The adsorption efficiency of this polymer was investigated for the removal of lead and cadmium ions pollutants. The adsorption processes of Pb(II) and Cd(II) metal ions by this polymer adsorbent were exothermic and matched by the Langmuir isotherm with a high correlation coefficient (R2 = 0.9904, 0.9778), the maximum adsorption capacity (833.33, 178.57 mg g−1), and the pseudo-second-order kinetic model. Furthermore, NR-POP showed an excellent adsorption selectivity for Pb(II) compared to Cd(II). [ABSTRACT FROM AUTHOR]

Sorption is prominent in low price, high efficiency, availability, and eco-friendliness. Organic porous materials have the characteristics of easy functionalization, diverse structure and stability, and show great potential in adsorption, energy storage, catalysis, and other fields. A mesoporous phenolic resin-type polymer (PRP) was successfully synthesized and modified by solid state reaction with maleic anhydride to prepare adsorbent (called as PRP-MAH) for sorption of Pb2+. The impact of reaction conditions (the pH value, reaction temperature, fresh concentration of solution, ionic strength and reaction time, etc.) was systematically studied. Characterization methods such as SEM, FTIR, and XPS indicated that the synthesized adsorbent PRP-MAH had regular morphology and good stability. The fitting of isothermal adsorption experiment data conforms to Langmuir sorption isotherm, and the sorption capacity reached 366.40 mg·g−1 at 308 K. The kinetic data were consistent with the quasi-second-order model, which indicated that the chemisorption might play the main role in the sorption process. Thermodynamic research manifested that the sorption of Pb2+ by PRP-MAH was carried out by a spontaneous process at the study temperature. The studies show that PRP-MAH can remove Pb2+ from water solution through ion exchange, electrostatic attraction, and surface complexation. [ABSTRACT FROM AUTHOR]

Kaolinite is a widely available and inexpensive substance that has been successfully employed to decontaminate different hazardous contaminants from wastewater by adsorption. In this study, halloysite-like nanotubes (HNTs) were prepared via intercalation followed by ultrasonic rolling of the kaolinite layers. Then, maghemite nanoparticles were co-precipitated within the HNTs matrix to produce a maghemite/HNTs nanocomposite (MG@HNTs). XRD, SEM, TEM, FTIR, and a BET analyzer were used to characterize the MG@HNTs nanocomposite, which was then utilized to eliminate Cd(II) from aqueous solutions (water and wastewater). Using a batch methodology, the impact of various adsorption parameters on Cd(II) removal was explored. MG@HNTs nanocomposite exhibited a high adsorption capacity (qe) of 264.47 mg g−1 for Cd (II). The kinetic data well conformed to pseudo-2nd-order, while the adsorption isotherms conformed to the Langmuir model. The desorption study demonstrated that the MG@HNTs nanocomposite could be successfully regenerated and recycled five times, without losing its original removal efficiency. Furthermore, MG@HNTs exhibited effective implementation to remove cadmium from real water samples, including tap and industrial wastewater. This study confirmed the successful application of MG@HNTs as an efficient, eco-friendly, and sustainable adsorbent at a high level of feasibility for the uptake of hazardous contaminants from industrial wastewater. [ABSTRACT FROM AUTHOR]

Several countries have shown an increased prevalence of drug resistance in animal production due to the indiscriminate use of antibiotics and antiparasitics in human and veterinary medicine. This article aims to review existing methods using naturally occurring essential oils (EOs) and their isolated compounds (EOCs) as alternatives to antimicrobials and antiparasitic compounds in animal production and, consequently, to avoid resistance. The most-reported mechanism of action of EOs and EOCs was cell membrane damage, which leads to the leakage of cytoplasmic content, increased membrane permeability, inhibition of metabolic and genetic pathways, morphologic changes, antibiofilm effects, and damage to the genetic material of infections. In parasites, anticoccidial effects, reduced motility, growth inhibition, and morphologic changes have been reported. Although these compounds regularly show a similar effect to those promoted by traditional drugs, the elucidation of their mechanisms of action is still scarce. The use of EOs and EOCs can also positively influence crucial parameters in animal production, such as body weight gain, feed conversion rate, and cholesterol reduction, which also positively impact meat quality. The application of EOs and EOCs is enhanced by their association with other natural compounds or even by the association with synthetic chemicals, which has been found to cause synergism in their antimicrobial effect. By reducing the effective therapeutical/prophylactic dose, the chances of off-flavors – the most common issue in EO and EOC application – is greatly mitigated. However, there is very little work on the combination of EOs and EOCs in large in vivo studies. In addition, research must apply the correct methodology to properly understand the observed effects; for example, the use of only high concentrations may mask potential results obtained at lower dosages. Such corrections will also allow the elucidation of finer mechanisms and promote better biotechnologic use of EOs and EOCs. This manuscript presents several information gaps to be filled before the use of EOs and EOCs are fully applicable in animal production. [ABSTRACT FROM AUTHOR]

Nanotechnology has extensive application as nanomedicine in the medical field. Some nanoparticles have possible applications in novel diagnostic instruments, imagery and methodologies, targeted medicinal products, pharmaceutical products, biomedical implants, and tissue engineering. Today treatments of high toxicity can be administered with improved safety using nanotechnology, such as chemotherapeutic cancer drugs. Further, wearable gadgets can detect crucial changes in vital signs, cancer cell conditions, and infections that are genuinely happening in the body. We anticipate these technologies to provide doctors with considerably much better direct access to critical data on the reasons for changes in the signs of life or illness because of the technological presence at the source of the problem. Biomedicine can be utilised for therapies with predictive analytics and artificial intelligence. For carrying out this study, relevant papers on Nanotechnology in the medical field from Scopus, Google scholar, ResearchGate, and other research platforms are identified and studied. The study discusses different types of Nanoparticles used in the medical field. This paper discusses nanotechnology applications in the medical field. The class, features, and characteristics of Nanotechnology for medicine are also briefed. Scientists, governments, civil society organisations, and the general public will need to collaborate across sectors to assess the significance of nanotechnology and guide its advancement in various fields. The current research includes several possible Nanotechnology uses in the medical field. As a result, the study provides a brief and well-organised report on nanotechnology that should be valuable to researchers, engineers, and scientists for future research projects. [ABSTRACT FROM AUTHOR]

Pharmacogenetics (PGx)-guided prescribing is an evidence-based precision medicine strategy. Although the past two decades have reported significant advancements in both the quality and quantity of PGx research studies, they are seldom done in developing countries like Bangladesh. This review identified and summarized PGx studies conducted in the Bangladeshi population by searching PubMed and Google Scholar. Additionally, a quality evaluation of the identified studies was also carried out. Eleven PGx studies were identified that looked at the effects of genetic variants on blood thinners (CYP2C9, VKORC1, and ITGB3), cancer drugs (TPMT, MTHFR, DPYD, ERCC1, GSTP1, XPC, XRCC1, TP53, XPD, and ABCC4), statins (COQ2, CYP2D6, and CYP3A5), and prednisolone (ABCB1, CYP3A5, and NR3C1) in the Bangladeshi population. Most studies were of low to moderate quality. Although the identified studies demonstrated the potential for PGx testing, the limited PGx literature in the Bangladeshi population poses a significant challenge in the widespread implementation of PGx testing in Bangladesh. [ABSTRACT FROM AUTHOR]

Water logging is one of the most detrimental phenomena continuing to burden Dhaka dwellers. This study aims to spatio-temporarily identify the water logging hazard zones within Dhaka Metropolitan area and assess the extent of their water logging susceptibility based on informal settlements, built-up areas, and demographical characteristics. The study utilizes integrated geographic information system (GIS)-remote sensing (RS) methods, using the Normalized Difference Vegetation Water and Moisture Index, distance buffer zone from drainage streams, and built-up distributions to identify waterlogged zones with a temporal extent, incorporating social and infrastructural attributes to evaluate water logging effects. These indicators were integrated into an overlay GIS method to measure the vulnerability level across Dhaka city areas. The findings reveal that south and south-western parts of Dhaka were more susceptible to water logging hazards. Almost 35% of Dhaka belongs to the high/very highly vulnerable zone. Greater number of slum households were found within high to very high water logging vulnerable zones and approximately 70% of them are poorly structured. The built-up areas were observed to be increased toward the northern part of Dhaka and were exposed to severe water logging issues. The overall findings reveal the spatio-temporal distribution of the water logging vulnerabilities across the city as well as its impact on the social indicators. An integrated approach is necessary for future development plans to mitigate the risk of water logging. [ABSTRACT FROM AUTHOR]

Phosphorus (P) deficiency stress in combination with biotic stress(es) severely impacts crop yield. Plant responses to P deficiency overlapping with that of other stresses exhibit a high degree of complexity involving different signaling pathways. On the one hand, plants engage with rhizosphere microbiome/arbuscular mycorrhizal fungi for improved phosphate (Pi) acquisition and plant stress response upon Pi deficiency; on the other hand, this association is gets disturbed under Pi sufficiency. This nutrient-dependent response is highly regulated by the phosphate starvation response (PSR) mediated by the master regulator, PHR1 , and its homolog, PHL. It is interesting to note that Pi status (deficiency/sufficiency) has a varying response (positive/negative) to different biotic encounters (beneficial microbes/opportunistic pathogens/insect herbivory) through a coupled PSR– PHR1 immune system. This also involves crosstalk among multiple players including transcription factors, defense hormones, miRNAs, and Pi transporters, among others influencing the plant-biotic–phosphate interactions. We provide a comprehensive view of these key players involved in maintaining a delicate balance between Pi homeostasis and plant immunity. Finally, we propose strategies to utilize this information to improve crop resilience to Pi deficiency in combination with biotic stresses. [ABSTRACT FROM AUTHOR]

A reduced graphene oxide (RGO) supported Fe3O4-MnO2 nanocomposite (Fe3O4-MnO2@RGO) was successfully prepared for catalytic degradation of oxytetracycline (20 mg/L) by potassium persulfate (PS) and adsorption removal of mixture of Pb2+, Cu2+, and Cd2+ ions (each 0.2 mM) in the synchronous scenario. The removal efficiencies of oxytetracycline, Pb2+, Cu2+, and Cd2+ ions were observed as high as 100%, 99.9%, 99.8%, and 99.8%, respectively, under the conditions of [PS]0 = 4 mM, pH0 = 7.0, Fe3O4-MnO2@RGO dosage = 0.8 g/L, reaction time = 90 min. The ternary composite exhibited higher oxytetracycline degradation/mineralization efficiency, greater metal adsorption capacity (Cd2+ 104.1 mg/g, Pb2+ 206.8 mg/g, Cu2+ 70.2 mg/g), and better PS utilization (62.6%) than its unary and binary counterparts including RGO, Fe3O4, Fe3O4@RGO, and Fe3O4-MnO2. More importantly, the ternary composite had good magnetic recoverability and excellent reusability. Notably, Fe, Mn, and RGO could play a synergistic role in the improvement of pollutant removal. Quenching results indicate that surface bounded SO4•– was the major contributor to oxytetracycline decomposition, and the -OH groups on the composite surface shouldered a significant role in PS activation. The results indicate that the magnetic Fe3O4-MnO2@RGO nanocomposite has a good potential for removing organic-metal co-contaminants in waterbody. [ABSTRACT FROM AUTHOR]