101. Excessive Accumulation of Intracellular Ca2+ After Acute Exercise Potentiated Impairment of T-cell Function
- Author
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Renyi Liu, Karsten Krüger, Christian Pilat, Wei Fan, Yu Xiao, Michael Seimetz, Robert Ringseis, Eveline Baumgart-Vogt, Klaus Eder, Norbert Weissmann, and Frank Christoph Mooren
- Subjects
acute exercise ,Fura-2(AM) ,intracellular Ca2+ ,calcium homeostasis ,cell proliferation ,calcium channels ,Physiology ,QP1-981 - Abstract
Ca2+ is an important intracellular second messenger known to regulate several cellular functions. This research aimed to investigate the mechanisms of exercise-induced immunosuppression by measuring intracellular calcium levels, Ca2+-regulating gene expression, and agonist-evoked proliferation of murine splenic T lymphocytes. Mice were randomly assigned to the control, sedentary group (C), and three experimental groups, which performed a single bout of intensive and exhaustive treadmill exercise. Murine splenic lymphocytes were separated by density-gradient centrifugation immediately (E0), 3h (E3), and 24h after exercise (E24). Fura-2/AM was used to monitor cytoplasmic free Ca2+ concentration in living cells. The combined method of carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling and flow cytometry was used for the detection of T cell proliferation. The transcriptional level of Ca2+-regulating genes was quantified by using qPCR. Both basal intracellular Ca2+ levels and agonist (ConA, OKT3, or thapsigargin)-induced Ca2+ transients were significantly elevated at E3 group (p
- Published
- 2021
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