121 results on '"Kakigi, Ryo"'
Search Results
102. Alpha-actinin-3 levels increase concomitantly with fast fibers in rat soleus muscle
- Author
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Ogura, Yuji, primary, Naito, Hisashi, additional, Kakigi, Ryo, additional, Ichinoseki-Sekine, Noriko, additional, Kurosaka, Mitsutoshi, additional, and Katamoto, Shizuo, additional
- Published
- 2008
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103. Effects of a lack of Snark on activity, heart rate, and fat and skeletal tissues in mice
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Ichinoseki‐Sekine, Noriko, primary, Naito, Hisashi, additional, Tsuchihara, Katsuya, additional, Kakigi, Ryo, additional, Ogura, Yuji, additional, Kurosaka, Mitsutoshi, additional, Katamoto, Shizuo, additional, and Esumi, Hiroyasu, additional
- Published
- 2008
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104. α‐Actinin‐3 increases concomitantly with skeletal muscle fast fibers in rat soleus muscle
- Author
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Ogura, Yuji, primary, Naito, Hisashi, additional, Ichinoseki‐Sekine, Noriko, additional, Kurosaka, Mitsutoshi, additional, Kakigi, Ryo, additional, and Katamoto, Shizuo, additional
- Published
- 2008
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105. Acute exercise‐induced heat shock proteins do not protect heart from hypoxia‐reoxygenation injury in adult rat
- Author
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Tsujikawa, Hiroto, primary, Ogura, Yuji, additional, Kakigi, Ryo, additional, Kurosaka, Mitsutoshi, additional, Naito, Hisashi, additional, Watanabe, Makino, additional, Katamoto, Shizuo, additional, and Okada, Takao, additional
- Published
- 2008
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106. Genotype Score for Iron Status Is Associated with Muscle Fiber Composition in Women.
- Author
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Takaragawa, Mizuki, Tobina, Takuro, Shiose, Keisuke, Kakigi, Ryo, Tsuzuki, Takamasa, Ichinoseki-Sekine, Noriko, Kumagai, Hiroshi, Zempo, Hirofumi, Miyamoto-Mikami, Eri, Kobayashi, Hiroyuki, Naito, Hisashi, and Fuku, Noriyuki
- Subjects
IRON in the body ,IRON deficiency ,GENOTYPES ,FIBERS ,RATS ,MYOSIN - Abstract
Human muscle fiber composition is heterogeneous and mainly determined by genetic factors. A previous study reported that experimentally induced iron deficiency in rats increases the proportion of fast-twitch muscle fibers. Iron status has been reported to be affected by genetic factors. As the TMPRSS6 rs855791 T/C and HFE rs1799945 C/G polymorphisms are strongly associated with iron status in humans, we hypothesized that the genotype score (GS) based on these polymorphisms could be associated with the muscle fiber composition in humans. Herein, we examined 214 Japanese individuals, comprising of 107 men and 107 women, for possible associations of the GS for iron status with the proportion of myosin heavy chain (MHC) isoforms (I, IIa, and IIx) as markers of muscle fiber composition. No statistically significant correlations were found between the GS for iron status and the proportion of MHC isoforms in all participants. When the participants were stratified based on sex, women showed positive and negative correlations of the GS with MHC-IIa (age-adjusted p = 0.020) and MHC-IIx (age-adjusted p = 0.011), respectively. In contrast, no correlation was found in men. In women, a 1-point increase in the GS was associated with 2.42% higher MHC-IIa level and 2.72% lower MHC-IIx level. Our results suggest that the GS based on the TMPRSS6 rs855791 T/C and HFE rs1799945 C/G polymorphisms for iron status is associated with muscle fiber composition in women. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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107. Whey Peptides Intake activates mTOR Signaling after Resistance Exercise Independent of Sex and Menstrual Cycle: 2264 Board #100 June 1 9:30 AM - 11:00 AM.
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Kakigi, Ryo, Ichinoseki-Sekine, Noriko, Natsume, Toshiharu, Kitada, Tomoharu, Yoshihara, Toshinori, Tsuzuki, Takamasa, Kobayashi, Hiroyuki, Machida, Shuichi, and Naito, Hisashi
- Subjects
- *
CONFERENCES & conventions , *GROWTH factors , *RESISTANCE training - Published
- 2018
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108. Long-term Physical Inactivity Exacerbates Hindlimb Unloading-induced Soleus Muscle Atrophy In Young Rats: 3271 Board #140 June 2 9:30 AM - 11:00 AM.
- Author
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Yoshihara, Toshinori, Tsuzuki, Takamasa, Shuo-wen, Chang, Natsume, Toshiharu, Kakigi, Ryo, Ichinoseki-Sekine, Noriko, Machida, Shuichi, Sugiura, Takao, and Naito, Hisashi
- Published
- 2018
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109. The expressions of alpha-actinin isoforms in rat damaged muscle.
- Author
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KAKIGI, Ryo, NAITO, Hisashi, OGURA, Yuji, FUKA1, Yuto, YOSHIHARA, Toshinori, and KATAMOTO, Shizuo
- Abstract
An abstract of the study "The expressions of alpha-actinin isoforms in rat damaged muscle," by Ryo Kakigi and colleagues is presented.
- Published
- 2010
110. Whey Peptide HW-3 enhances the recovery from muscle atrophy in rat.
- Author
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ICHINOSEKI-SEKINE, Noriko, KAKIGI, Ryo, OGURA, Yuji, MIURA, Susumu, and NAITO, Hisashi
- Abstract
An abstract of the study "Whey Peptide HW-3 enhances the recovery from muscle atrophy in rat," by Hisashi Naito and colleagues is presented.
- Published
- 2009
111. Effects of heat stress on mTOR signaling pathway in rat skeletal muscle.
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KAKIGI, Ryo, NAITO, Hisashi, OGURA, Yuji, and KATAMOTO, Shizuo
- Abstract
An abstract of the study "Effects of heat stress on mTOR signaling pathway in rat skeletal muscle," by Ryo Kakigi and colleagues is presented.
- Published
- 2009
112. A chronic high-fat diet exacerbates contractile dysfunction with impaired intracellular Ca 2+ release capacity in the skeletal muscle of aged mice.
- Author
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Eshima H, Tamura Y, Kakehi S, Kakigi R, Hashimoto R, Funai K, Kawamori R, and Watada H
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- Animals, Male, Mice, Mice, Inbred C57BL, Muscle, Skeletal, Obesity, Diet, High-Fat adverse effects, Muscle Contraction
- Abstract
Obesity and aging reduce skeletal muscle contractile function. However, it remains unclear whether obesity additively promotes muscle contractile dysfunction in the setting of aging. In this study, we investigated skeletal muscle contractile function ex vivo and intracellular Ca
2+ release in male C57BL/6J mice fed a low-fat diet (LFD) or a high-fat diet (HFD) for 4 or 20 mo. Tetanic force production in the extensor digitorum longus muscle was decreased by aging or HFD feeding, and the further reduction was observed in aged HFD mice. The 20-mo HFD-fed mice, not the 20-mo LFD-fed mice or 4-mo HFD-fed mice, showed reduced intracellular Ca2+ peak levels by high concentration of caffeine (25 mM) compared with 4-mo LFD mice. Aging and HFD feeding additively increased intramyocellular lipid (IMCL) levels and were associated with the degree of impaired muscle contractile force and peak Ca2+ level. These data suggest that impairment in the contractile force in aged muscle is aggravated by HFD, which may be due, at least in part, to dysfunction in intracellular Ca2+ release. The IMCL level may be a marker for impaired muscle contractile force caused by aging and HFD. NEW & NOTEWORTHY The aim of this study was to examine the effect of high-fat diet (HFD)-induced obesity on contractile function and Ca2+ release capacity in aged skeletal muscle. Not only were the force production and peak Ca2+ levels decreased by aging and HFD feeding, respectively, but also, these interventions had an additive effect in aged HFD-fed mice. These data suggest that the impairment in the contractile force in aged muscle is aggravated by a HFD, which may be due to synergistic dysfunction in intracellular Ca2+ release.- Published
- 2020
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113. Sex-specific differences in rat soleus muscle signaling pathway responses to a bout of horizontal and downhill running.
- Author
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Yoshihara T, Chang SW, Tsuzuki T, Natsume T, Kakigi R, Sugiura T, and Naito H
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- Adaptation, Physiological, Animals, Female, Male, Physical Conditioning, Animal, Rats, Rats, Wistar, Sex Characteristics, Extracellular Signal-Regulated MAP Kinases metabolism, HSP72 Heat-Shock Proteins metabolism, Muscle, Skeletal metabolism, Myostatin metabolism, Physical Exertion physiology, TOR Serine-Threonine Kinases metabolism
- Abstract
Males and females of many species, including humans, exhibit different muscle responses and adaptations to exercise stress; however, the molecular mechanisms that underlie these changes are poorly understood. Therefore, the present study assessed sex-related differences in intracellular signaling pathway responses to bouts of horizontal or downhill running in rat soleus muscles. Age-matched male and female Wistar rats (10 weeks old, n = 18/group) were either rested (control group) or subjected to an either a bout of horizontal (22 m/min, 20 min, 0° incline) or downhill (16 m/min, 10 min, - 16% incline) treadmill running. Soleus muscle samples were collected both prior to and immediately after exercise (n = 6/group). Intramuscular signaling responses to each type of exercise were determined via real-time (RT) PCR and western blot analyses. Although mTOR signaling (mTOR/S6K1/S6) responses to both horizontal and downhill exercise were found to be similar in both sexes, ERK phosphorylation levels were found to be significantly higher in male than in female rats after downhill exercise. Similarly, heat shock protein (Hsp) 72 and myostatin protein expression levels were both found to be significantly altered after downhill exercise: Hsp levels increased in male and decreased in female rats, whereas myostatin increased in female but decreased in male rats. Thus, the results of the present study suggest that downhill exercise may elicit sex-specific differential changes to Hsp72 expression, ERK phosphorylation, and myostatin-signaling activation in female compared with those in male rat soleus muscles. Further study is required to confirm these findings and to determine the way in which they impact sex-specific differences in exercise-induced muscle adaptations.
- Published
- 2019
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114. Sex differences in forkhead box O3a signaling response to hindlimb unloading in rat soleus muscle.
- Author
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Yoshihara T, Natsume T, Tsuzuki T, Chang SW, Kakigi R, Sugiura T, and Naito H
- Subjects
- Animals, Female, HSP72 Heat-Shock Proteins metabolism, Hindlimb Suspension physiology, Male, Myostatin metabolism, Phosphorylation physiology, Proteasome Endopeptidase Complex metabolism, Rats, Rats, Wistar, Sex Characteristics, Ubiquitin metabolism, Forkhead Box Protein O3 metabolism, Hindlimb metabolism, Hindlimb physiology, Muscle, Skeletal metabolism, Muscle, Skeletal physiology, Signal Transduction physiology
- Abstract
We tested the hypothesis that there are sex differences in hindlimb unloading-induced activation of the forkhead box subfamily O3a (FoxO3a) signaling pathway in rat soleus muscle. Age-matched male and female Wistar rats were subjected to hindlimb unloading, and the soleus muscle was removed before or 1 or 7 days after unloading. Female rats showed greater percent changes in relative soleus muscle weight than males. FoxO3a phosphorylation was lower in females than in males and was associated with higher levels of protein ubiquitination 7 days after unloading. Heat shock protein 72 (Hsp72) levels were lower in female rats and increased in males during unloading. Female rats showed slightly higher myostatin levels, which showed a non-significant decline in male rats following unloading. Thus, males and females show different responses to the FoxO3a/ubiquitin-proteasome pathway following hindlimb unloading in rat soleus muscle, which may be associated with differences in Hsp72 expression and myostatin signaling.
- Published
- 2019
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115. Dysfunction of muscle contraction with impaired intracellular Ca 2+ handling in skeletal muscle and the effect of exercise training in male db/db mice.
- Author
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Eshima H, Tamura Y, Kakehi S, Nakamura K, Kurebayashi N, Murayama T, Kakigi R, Sakurai T, Kawamori R, and Watada H
- Subjects
- Animals, Male, Mice, Running physiology, Calcium metabolism, Diabetes Mellitus, Experimental physiopathology, Muscle Contraction, Muscle, Skeletal physiopathology, Physical Conditioning, Animal physiology
- Abstract
Type 2 diabetes is characterized by reduced contractile force production and increased fatigability of skeletal muscle. While the maintenance of Ca
2+ homeostasis during muscle contraction is a requisite for optimal contractile function, the mechanisms underlying muscle contractile dysfunction in type 2 diabetes are unclear. Here, we investigated skeletal muscle contractile force and Ca2+ flux during contraction and pharmacological stimulation in type 2 diabetic model mice ( db/db mice). Furthermore, we investigated the effect of treadmill exercise training on muscle contractile function. In male db/db mice, muscle contractile force and peak Ca2+ levels were both lower during tetanic stimulation of the fast-twitch muscles, while Ca2+ accumulation was higher after stimulation compared with control mice. While 6 wk of exercise training did not improve glucose tolerance, exercise did improve muscle contractile dysfunction, peak Ca2+ levels, and Ca2+ accumulation following stimulation in male db/db mice. These data suggest that dysfunctional Ca2+ flux may contribute to skeletal muscle contractile dysfunction in type 2 diabetes and that exercise training may be a promising therapeutic approach for dysfunctional skeletal muscle contraction. NEW & NOTEWORTHY The purpose of this study was to examine muscle contractile function and Ca2+ regulation as well as the effect of exercise training in skeletal muscle in obese diabetic mice ( db/db). We observed impairment of muscle contractile force and Ca2+ regulation in a male type 2 diabetic animal model. These dysfunctions in muscle were improved by 6 wk of exercise training.- Published
- 2019
- Full Text
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116. Effect of a combination of astaxanthin supplementation, heat stress, and intermittent reloading on satellite cells during disuse muscle atrophy.
- Author
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Yoshihara T, Sugiura T, Miyaji N, Yamamoto Y, Shibaguchi T, Kakigi R, Naito H, Goto K, Ohmori D, and Yoshioka T
- Subjects
- Animals, Body Weight, Fibrinolytic Agents pharmacology, Hindlimb, Hot Temperature, Immunohistochemistry, Male, Muscle, Skeletal, Oxidative Stress, Rats, Rats, Wistar, Xanthophylls pharmacology, Dietary Supplements, Heat-Shock Response, Muscular Atrophy drug therapy, Satellite Cells, Skeletal Muscle cytology
- Abstract
We examined the effect of a combination of astaxanthin (AX) supplementation, repeated heat stress, and intermittent reloading (IR) on satellite cells in unloaded rat soleus muscles. Forty-nine male Wistar rats (8-week-old) were divided into control, hind-limb unweighting (HU), IR during HU, IR with AX supplementation, IR with repeated heat stress (41.0-41.5 °C for 30 min), and IR with AX supplementation and repeated heat stress groups. After the experimental period, the antigravitational soleus muscle was analyzed using an immunohistochemical technique. Our results revealed that the combination of dietary AX supplementation and heat stress resulted in protection against disuse muscle atrophy in the soleus muscle. This protective effect may be partially due to a higher satellite cell number in the atrophied soleus muscle in the IR/AX/heat stress group compared with the numbers found in the other groups. We concluded that the combination treatment with dietary AX supplementation and repeated heat stress attenuates soleus muscle atrophy, in part by increasing the number of satellite cells.
- Published
- 2018
- Full Text
- View/download PDF
117. Effects of training intensity in electromyostimulation on human skeletal muscle.
- Author
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Natsume T, Ozaki H, Kakigi R, Kobayashi H, and Naito H
- Subjects
- Adult, Electric Stimulation, Humans, Leg physiology, Male, Isometric Contraction, Muscle Fibers, Skeletal physiology, Physical Conditioning, Human methods
- Abstract
Purpose: High-intensity neuromuscular electrical stimulation (NMES) training can induce muscle hypertrophy at the whole muscle and muscle fiber levels. However, whether low-intensity NMES training has a similar result is unknown. This study aimed to investigate whether low-intensity NMES training could elicit muscle hypertrophy at the whole muscle and muscle fiber levels in the human skeletal muscle., Methods: Eight untrained young males were subjected to 18 min of unilateral NMES training for 8 weeks. One leg received NMES at maximal tolerable intensity (HIGH); the other leg received NMES at an intensity half of that in the HIGH condition (LOW). Quadriceps muscle thickness (MT), muscle fiber cross-sectional area (CSA), and knee extension strength were measured before and after the training period., Results: The average training intensity throughout the intervention period in the HIGH and LOW conditions were 62.5 ± 4.6% maximal voluntary contraction (MVC) and 32.6 ± 2.6% MVC, respectively. MT, CSA, and muscle strength increased in both exercise conditions (p < 0.05); however, training effects in the LOW condition were lower than those in the HIGH condition (p < 0.05). The average training intensity showed a positive correlation with percent changes in muscle strength (r = 0.797, p = 0.001), MT (r = 0.876, p = 0.001), type I fiber CSA (r = 0.730, p = 0.01), and type II fiber CSA (r = 0.899, p = 0.001)., Conclusions: Low-intensity NMES could increase MT, muscle fiber CSA, and muscle strength in healthy human skeletal muscles. However, the magnitude of increase is lower in low-intensity than in high-intensity NMES training.
- Published
- 2018
- Full Text
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118. LPS enhances expression of CD204 through the MAPK/ERK pathway in murine bone marrow macrophages.
- Author
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Hashimoto R, Kakigi R, Nakamura K, Itoh S, Daida H, Okada T, and Katoh Y
- Subjects
- Animals, Atherosclerosis drug therapy, Atherosclerosis enzymology, Atherosclerosis immunology, CD36 Antigens metabolism, Cells, Cultured, Enzyme Activation, Extracellular Signal-Regulated MAP Kinases antagonists & inhibitors, Lipoproteins, LDL metabolism, Macrophages enzymology, Macrophages immunology, Male, Mice, Inbred C57BL, Phosphorylation, Protein Kinase Inhibitors pharmacology, Signal Transduction drug effects, Up-Regulation, Atherosclerosis chemically induced, Extracellular Signal-Regulated MAP Kinases metabolism, Lipopolysaccharides pharmacology, Macrophages drug effects, Scavenger Receptors, Class A metabolism
- Abstract
Background and Aims: Lipopolysaccharide (LPS) is a main component of the Gram-negative bacterial cell wall and is associated with a greater risk of atherosclerosis development in periodontal disease. LPS has been reported to increase both CD36 and CD204 expression and enhance the uptake of modified low-density lipoprotein (LDL). However, the signaling pathways by which LPS enhances these expression levels and function have not been fully elucidated, although the clarification of these signaling pathways is important for identifying therapeutic targets for atherosclerosis., Methods and Results: We have shown here that LPS activated the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway, increased both CD204 and CD36 expression, and enhanced the uptake of acetylated-LDL (Ac-LDL) in mouse bone marrow macrophages. The MAPK/ERK kinase (MEK) inhibitors, U0126 (1 μM) and PD0325901 (10 nM), did not affect the expression of either CD36 or CD204 or the uptake of Ac-LDL under normal conditions (no treatment with LPS). In contrast, U0126 (1 μM) and PD0325901 (10 nM) blocked the LPS-induced increase in Ac-LDL uptake and CD204 expression but not CD36 expression., Conclusions: These results suggest that LPS may increase Ac-LDL uptake and enhance CD204 expression through MAPK/ERK activation and CD36 expression through an ERK-independent pathway. Since MEK inhibitors block CD204 expression in mouse BM macrophages only under LPS treatment but not under normal conditions, a MEK inhibitor might be a good candidate compound for the treatment of LPS-induced atherosclerosis., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
119. Short-term treadmill exercise in a cold environment does not induce adrenal Hsp72 and Hsp25 expression.
- Author
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Akin S, Naito H, Ogura Y, Ichinoseki-Sekine N, Kurosaka M, Kakigi R, and Demirel HA
- Subjects
- Adaptation, Physiological physiology, Adrenal Glands metabolism, Adrenal Glands physiology, Animals, Body Temperature physiology, Cold Temperature, Exercise Test methods, Female, Myocardium metabolism, Rats, Rats, Sprague-Dawley, Running physiology, HSP27 Heat-Shock Proteins metabolism, HSP72 Heat-Shock Proteins metabolism, Physical Conditioning, Animal physiology
- Abstract
Heat shock proteins (Hsps) have a critical role in maintaining cellular homeostasis and in protecting cells from a range of acute and chronic stressful conditions. Treadmill running exercise results in increased Hsp72 and Hsp25 levels in various tissues and heat production during exercise has been shown to be the main factor for the increased levels of Hsp72 in myocardium. Since the adrenal gland plays a vital role in general response to stress, regulation of Hsps in adrenal glands following stressful events seems to be critical for controlling the whole-body stress response appropriately. This study tested the hypothesis of whether elevation of temperature is solely responsible for exercise-induced adrenal Hsp72 and Hsp25 expression. Female Sprague-Dawley rats (3 months old) were randomly assigned to either a sedentary control group or one of two treadmill-running groups: a cold exercise group run in a cold room at 4 °C (CE), and a warm exercise group run at 25 °C temperature (WE). Animals were run 60 min a day at 30 m min
-1 speed for 4 consecutive days following adaptation to treadmill exercise. Exercise resulted in a significant elevation of body temperature only in the WE group (p < 0.05). Adrenal Hsp72 and Hsp25 levels were significantly higher in the WE group compare to the other groups (p < 0.05). These data demonstrated that exercise-related elevations of body temperature could be the only factor for the inductions of adrenal Hsp72 and Hsp25 expression.- Published
- 2017
- Full Text
- View/download PDF
120. Attenuation of exercise-induced heat shock protein 72 expression blunts improvements in whole-body insulin resistance in rats with type 2 diabetes.
- Author
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Tsuzuki T, Kobayashi H, Yoshihara T, Kakigi R, Ichinoseki-Sekine N, and Naito H
- Subjects
- Animals, Area Under Curve, Body Temperature, Cholesterol blood, Diabetes Mellitus, Type 2 metabolism, Disease Models, Animal, Fatty Acids, Nonesterified blood, Glucose Tolerance Test, Insulin metabolism, Insulin Resistance, Liver metabolism, Muscle, Skeletal metabolism, Physical Conditioning, Animal, ROC Curve, Rats, Rats, Long-Evans, Temperature, Triglycerides blood, Diabetes Mellitus, Type 2 pathology, HSP72 Heat-Shock Proteins metabolism
- Abstract
Heat shock proteins (HSPs) play an important role in insulin resistance and improve the cellular stress response via HSP induction by exercise to treat type 2 diabetes. In this study, the effects of exercise-induced HSP72 expression levels on whole-body insulin resistance in type 2 diabetic rats were investigated. Male 25-week-old Otsuka Long-Evans Tokushima Fatty rats were divided into three groups: sedentary (Sed), trained in a thermal-neutral environment (NTr: 25 °C), and trained in a cold environment (CTr: 4 °C). Exercise training was conducted 5 days/week for 10 weeks. Rectal temperature was measured following each bout of exercise. An intraperitoneal glucose tolerance test (IPGTT) was performed after the training sessions. The serum, gastrocnemius muscle, and liver were sampled 48 h after the final exercise session. HSP72 and heat shock cognate protein 73 expression levels were analyzed by Western blot, and serum total cholesterol, triglyceride (TG), and free fatty acid (FFA) levels were measured. NTr animals exhibited significantly higher body temperatures following exercise, whereas, CTr animals did not. Exercise training increased HSP72 levels in the gastrocnemius muscle and liver, whereas, HSP72 expression was significantly lower in the CTr group than that in the NTr group (p < 0.05). Glucose tolerance improved equally in both trained animals; however, insulin levels during the IPGTT were higher in CTr animals than those in NTr animals (p < 0.05). In addition, the TG and FFA levels decreased significantly only in NTr animals compared with those in Sed animals. These results suggest that attenuation of exercise-induced HSP72 expression partially blunts improvement in whole-body insulin resistance and lipid metabolism in type 2 diabetic rats.
- Published
- 2017
- Full Text
- View/download PDF
121. Dietary astaxanthin supplementation attenuates disuse-induced muscle atrophy and myonuclear apoptosis in the rat soleus muscle.
- Author
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Yoshihara T, Yamamoto Y, Shibaguchi T, Miyaji N, Kakigi R, Naito H, Goto K, Ohmori D, Yoshioka T, and Sugiura T
- Subjects
- Animals, Antioxidants therapeutic use, Hindlimb Suspension physiology, Male, Muscle, Skeletal pathology, Muscular Atrophy pathology, Oxidative Stress drug effects, Rats, Rats, Wistar, Xanthophylls pharmacology, Xanthophylls therapeutic use, Antioxidants pharmacology, Apoptosis drug effects, Muscle, Skeletal drug effects, Muscular Atrophy drug therapy
- Abstract
Extended periods of skeletal muscle disuse results in muscle atrophy and weakness. Currently, no therapeutic treatment is available for the prevention of this problem. Nonetheless, growing evidence suggests that prevention of disuse-induced oxidative stress in inactive muscle fibers can delay inactivity-induced muscle wasting. Therefore, this study tested the hypothesis that dietary supplementation with the antioxidant astaxanthin would protect against disuse muscle atrophy, in part, by prevention of myonuclear apoptosis. Wistar rats (8 weeks old) were divided into control (CT, n = 9), hindlimb unloading (HU, n = 9), and hindlimb unloading with astaxanthin (HU + AX, n = 9) groups. Following 2 weeks of dietary supplementation, rats in the HU and HU + AX groups were exposed to unloading for 7 days. Seven-day unloading resulted in reduced soleus muscle weight and myofiber cross-sectional area (CSA) by ~30 and ~47 %, respectively. Nonetheless, relative muscle weights and CSA of the soleus muscle in the HU + AX group were significantly greater than those of the HU group. Moreover, astaxanthin prevented disuse-induced increase in the number of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive nuclei. We conclude that astaxanthin supplementation prior to and during hindlimb unloading attenuates soleus muscle atrophy, in part, by suppressing myonuclear apoptosis.
- Published
- 2017
- Full Text
- View/download PDF
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