101. Inter- and intra-organ spatial distributions of sea star saponins by MALDI imaging
- Author
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Maxence Wisztorski, Igor Eeckhaut, Marie Demeyer, Guillaume Caulier, Isabelle Fournier, Julien De Winter, Pascal Gerbaux, Patrick Flammang, Corentin Decroo, Elise Hennebert, Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 (PRISM), Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)
- Subjects
MALDI imaging ,[SDV]Life Sciences [q-bio] ,Saponin ,Mass spectrometry ,complex mixtures ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,03 medical and health sciences ,parasitic diseases ,Animals ,Direct analysis ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Chromatography ,biology ,010405 organic chemistry ,Chemistry ,Asterias ,Saponins ,musculoskeletal system ,biology.organism_classification ,Combinatorial chemistry ,0104 chemical sciences ,carbohydrates (lipids) ,Tissue sections ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Mucus layer - Abstract
Saponins are secondary metabolites that are abundant and diversified in echinoderms. Mass spectrometry is increasingly used not only to identify saponin congeners within animal extracts but also to decipher the structure/biological activity relationships of these molecules by determining their inter-organ and inter-individual variability. The usual method requires extensive purification procedures to prepare saponin extracts compatible with mass spectrometry analysis. Here, we selected the sea star Asterias rubens as a model animal to prove that direct analysis of saponins can be performed on tissue sections. We also demonstrated that carboxymethyl cellulose can be used as an embedding medium to facilitate the cryosectioning procedure. Matrix-assisted laser desorption/ionization (MALDI) imaging was also revealed to afford interesting data on the distribution of saponin molecules within the tissues. We indeed highlight that saponins are located not only inside the body wall of the animals but also within the mucus layer that probably protects the animal against external aggressions. Graphical Abstract Saponins are the most abundant secondary metabolites in sea stars. They should therefore participate in important biological activities. Here, MALDI imaging is presented as a powerful method to determine the spatial distribution of saponins within the animal tissues. The inhomogeneity of the intra-organ saponin distribution is highlighted, paving the way for future elegant structure/activity relationship investigations.
- Published
- 2015