1,012 results on '"Jiwon, Lee"'
Search Results
102. GROW: A Row-Stationary Sparse-Dense GEMM Accelerator for Memory-Efficient Graph Convolutional Neural Networks.
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Minhoo Kang, Ranggi Hwang, Jiwon Lee, Dongyun Kam, Youngjoo Lee, and Minsoo Rhu
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- 2022
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103. Hybrid Graph Models for Logic Optimization via Spatio-Temporal Information.
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Nan Wu, Jiwon Lee, Yuan Xie 0001, and Cong Hao
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- 2022
104. Deep-learning-based personalized prediction of absolute neutrophil count recovery and comparison with clinicians for validation.
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Hyunwoo Choo, Suyoung Yoo, Suhyeon Moon, Minsu Park, Jiwon Lee, Ki Woong Sung, Won Chul Cha, Soo-Yong Shin, and Meong Hi Son
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- 2023
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105. Visual modalities-based multimodal fusion for surgical phase recognition.
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Bogyu Park, Hyeon-Gyu Chi, Bokyung Park, Jiwon Lee, Hye Su Jin, Sunghyun Park, Woo Jin Hyung, and Min-Kook Choi
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- 2023
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106. Functional and Structural Changes in the Membrane-Bound O-Acyltransferase Family Member 7 (MBOAT7) Protein: The Pathomechanism of a Novel MBOAT7 Variant in Patients With Intellectual Disability
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Jiwon Lee, Amen Shamim, Jongho Park, Ja-Hyun Jang, Ji Hye Kim, Jeong-Yi Kwon, Jong-Won Kim, Kyeong Kyu Kim, and Jeehun Lee
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intellectual disability ,MBOAT7 ,autism spectrum disorder ,globus pallidus ,cerebellar dentate nucleus ,molecular modeling ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
The membrane-bound O-acyltransferase domain-containing 7 (MBOAT7) gene is associated with intellectual disability, early onset seizures, and autism spectrum disorders. This study aimed to determine the pathogenetic mechanism of the MBOAT7 missense variant via molecular modeling. Three patients from a consanguineous family were found to have a homozygous c.757G>A (p.Glu253Lys) variant of MBOAT7. The patients showed prominent dysfunction in gait, swallowing, vocalization, and fine motor function and had intellectual disabilities. Brain magnetic resonance imaging showed signal changes in the bilateral globus pallidi and cerebellar dentate nucleus, which differed with age. In the molecular model of human MBOAT7, Glu253 in the wild-type protein is located close to the backbone carbonyl oxygens in the loop near the helix, suggesting that the ionic interaction could contribute to the conformational stability of the funnel. Molecular modeling showed that Lys253 in the mutant protein was expected to alter the surface charge distribution, thereby potentially affecting substrate specificity. Changes in conformational stability and substrate specificity through varied ionic interactions are the suggested pathophysiological mechanisms of the MBOAT7 variant found in patients with intellectual disabilities.
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- 2022
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107. Boosting of cross-reactive antibodies to endemic coronaviruses by SARS-CoV-2 infection but not vaccination with stabilized spike
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Andrew R Crowley, Harini Natarajan, Andrew P Hederman, Carly A Bobak, Joshua A Weiner, Wendy Wieland-Alter, Jiwon Lee, Evan M Bloch, Aaron AR Tobian, Andrew D Redd, Joel N Blankson, Dana Wolf, Tessa Goetghebuer, Arnaud Marchant, Ruth I Connor, Peter F Wright, and Margaret E Ackerman
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SARS-CoV-2 ,antibodies ,vaccine ,epitope ,conformation ,neutralization ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Preexisting antibodies to endemic coronaviruses (CoV) that cross-react with SARS-CoV-2 have the potential to influence the antibody response to COVID-19 vaccination and infection for better or worse. In this observational study of mucosal and systemic humoral immunity in acutely infected, convalescent, and vaccinated subjects, we tested for cross-reactivity against endemic CoV spike (S) protein at subdomain resolution. Elevated responses, particularly to the β-CoV OC43, were observed in all natural infection cohorts tested and were correlated with the response to SARS-CoV-2. The kinetics of this response and isotypes involved suggest that infection boosts preexisting antibody lineages raised against prior endemic CoV exposure that cross-react. While further research is needed to discern whether this recalled response is desirable or detrimental, the boosted antibodies principally targeted the better-conserved S2 subdomain of the viral spike and were not associated with neutralization activity. In contrast, vaccination with a stabilized spike mRNA vaccine did not robustly boost cross-reactive antibodies, suggesting differing antigenicity and immunogenicity. In sum, this study provides evidence that antibodies targeting endemic CoV are robustly boosted in response to SARS-CoV-2 infection but not to vaccination with stabilized S, and that depending on conformation or other factors, the S2 subdomain of the spike protein triggers a rapidly recalled, IgG-dominated response that lacks neutralization activity.
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- 2022
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108. An Immunoproteomic Survey of the Antibody Landscape: Insights and Opportunities Revealed by Serological Repertoire Profiling
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Steven Ionov and Jiwon Lee
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Ig-Seq ,serological antibody repertoire ,proteomics ,infectious disease ,cancer ,autoimmunity ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Immunoproteomics has emerged as a versatile tool for analyzing the antibody repertoire in various disease contexts. Until recently, characterization of antibody molecules in biological fluids was limited to bulk serology, which identifies clinically relevant features of polyclonal antibody responses. The past decade, however, has seen the rise of mass-spectrometry-enabled proteomics methods that have allowed profiling of the antibody response at the molecular level, with the disease-specific serological repertoire elucidated in unprecedented detail. In this review, we present an up-to-date survey of insights into the disease-specific immunological repertoire by examining how quantitative proteomics-based approaches have shed light on the humoral immune response to infection and vaccination in pathogenic illnesses, the molecular basis of autoimmune disease, and the tumor-specific repertoire in cancer. We address limitations of this technology with a focus on emerging potential solutions and discuss the promise of high-resolution immunoproteomics in therapeutic discovery and novel vaccine design.
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- 2022
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109. Whole genome sequence analysis of pulmonary function and COPD in 19,996 multi-ethnic participants
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Xutong Zhao, Dandi Qiao, Chaojie Yang, Silva Kasela, Wonji Kim, Yanlin Ma, Nick Shrine, Chiara Batini, Tamar Sofer, Sarah A. Gagliano Taliun, Phuwanat Sakornsakolpat, Pallavi P. Balte, Dmitry Prokopenko, Bing Yu, Leslie A. Lange, Josée Dupuis, Brian E. Cade, Jiwon Lee, Sina A. Gharib, Michelle Daya, Cecelia A. Laurie, Ingo Ruczinski, L. Adrienne Cupples, Laura R. Loehr, Traci M. Bartz, Alanna C. Morrison, Bruce M. Psaty, Ramachandran S. Vasan, James G. Wilson, Kent D. Taylor, Peter Durda, W. Craig Johnson, Elaine Cornell, Xiuqing Guo, Yongmei Liu, Russell P. Tracy, Kristin G. Ardlie, François Aguet, David J. VanDenBerg, George J. Papanicolaou, Jerome I. Rotter, Kathleen C. Barnes, Deepti Jain, Deborah A. Nickerson, Donna M. Muzny, Ginger A. Metcalf, Harshavardhan Doddapaneni, Shannon Dugan-Perez, Namrata Gupta, Stacey Gabriel, Stephen S. Rich, George T. O’Connor, Susan Redline, Robert M. Reed, Cathy C. Laurie, Martha L. Daviglus, Liana K. Preudhomme, Kristin M. Burkart, Robert C. Kaplan, Louise V. Wain, Martin D. Tobin, Stephanie J. London, Tuuli Lappalainen, Elizabeth C. Oelsner, Goncalo R. Abecasis, Edwin K. Silverman, R. Graham Barr, NHLBI Trans-Omics for Precision Medicine (TOPMed) Consortium, TOPMed Lung Working Group, Michael H. Cho, and Ani Manichaikul
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Science - Abstract
Chronic obstructive pulmonary disease is a leading cause of morbidity and mortality. Here, the authors analyse whole genome sequence data and find new loci associated with lung function and COPD.
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- 2020
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110. Household waste separation intention and the importance of public policy
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Bing Chen and Jiwon Lee
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municipal solid waste ,household waste separation intention ,external stimuli ,internal stimuli ,municipal governments ,Regulation of industry, trade, and commerce. Occupational law ,K3840-4375 ,Economic growth, development, planning ,HD72-88 - Abstract
Purpose – The key challenge that urban cities in most developing and transitional economies is confronting is municipal solid waste (MSW) management. Waste separation is a critical component to successful recycling management in terms of enhancing the quality of recyclables, reducing MSW and optimizing incineration. The urge to actualizing sustained waste separation behavior has been hindered by potential barriers. This study aims to examine the influences of external and internal stimuli of targeted households' waste separation intention in parts of China. Design/methodology/approach – A multifactor framework predicting the process that leads to waste separation attitude and behavioral intention is proposed. SEM analysis is conducted in SmartPLS based on 371 survey questionnaires collected in Nanning city in China. Findings – Policy regulation is the biggest determinant of attitude among external stimuli, while awareness of consequence has the strongest relationship with an attitude among internal stimuli. Facilitating conditions, subjective norms and moral norms are all significant predictors of attitude. As a result, increasing positive attitude leads to enhance waste separation lifestyle. Research limitations/implications – This study adopts a cross-sectional design to investigate the waste separation intention of local households. Data collection is restricted to one point in time for every individual. A mixed method is recommended. Quantitative research can examine variables provided in existing literature with numerical analysis. Qualitative research might be helpful to identify other unknown factors. Also, the survey questionnaires employ a self-reported manner, and respondents might be overrating to avoid embarrassment. Practical implications – Future research is recommended to engage observation at houses or at the waste-collecting points for actual waste separation behavior. Moreover, this study measures intention toward household waste separation, but whether this intention will eventually lead to waste separation behavior is not a guarantee. Future study is recommended to examine whether intention translates into actual waste separation behavior. Originality/value – Emphasizing the importance of policy element as a direct influence toward attitude, this paper focuses on the waste separation attitude accumulated from external and internal stimuli, and, concurrently, waste separation behavioral intention is influenced by accumulated attitudes. The study provides relevant policy development information of three Asian countries to enhance their present and future policy directions for a sustainable household waste separation management process
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- 2020
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111. Nusinersen Administration in Spinal Muscular Atrophy Patients with Severe Scoliosis: Interlaminar Approaches at the Lumbar Level
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Joo Young Song, Hyun Su Kim, Se-Jun Park, Jiwon Lee, and Jeehun Lee
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fluoroscopy ,injections, spinal ,muscular atrophy, spinal ,scoliosis ,nusinersen ,Internal medicine ,RC31-1245 ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Purpose Spinal muscular atrophy (SMA) is a genetic progressive neuromuscular disorder, and nusinersen has shown to improve its symptoms. Scoliosis is a frequent symptom in patients with SMA and complicates the intrathecal injection of nusinersen. The aim of this study was to investigate the safety and effectiveness of fluoroscopy-guided intrathecal injections in patients with SMA with severe scoliosis. Methods We retrospectively reviewed the medical records of 11 patients with SMA treated with nusinersen intrathecal injections at the Samsung Medical Center from 2018 to 2020. We assessed the type of SMA, genetic results, spine computed tomography or fluoroscopy findings, and adverse effects associated with the injection. Results Of 11 patients with SMA, six were diagnosed with severe scoliosis, four of whom underwent an operation for scoliosis. The six patients with severe scoliosis started receiving the nusinersen injection at a median age of 15.7 years. A total of 25 injections were completely performed with the interlaminar approach (ILA) at the lumbar level under C-arm fluoroscopy guidance. No adverse effects other than mild headache occurred. In one patient who underwent the complete fusion operation for scoliosis, laminectomy was performed for the nusinersen injection, and 3 doses were administered intrathecally via the interlaminar route at the lumbar level. Conclusion Fluoroscopy-guided ILA is a safe method for the intrathecal injection of nusinersen in patients with SMA with severe scoliosis. When defining the route, laminectomy might be necessary to open the window for the ILA route at the lumbar level.
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- 2020
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112. Effects of simulation-based education for neonatal resuscitation on medical students' technical and non-technical skills.
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Jiwon Lee and Jang Hoon Lee
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Medicine ,Science - Abstract
Simulation is a learner-centered practice that helps develop and maintain knowledge, skills, and competencies. This study evaluated the effect of neonatal resuscitation simulation-based education for medical students in the fifth year (part of the regular clinical clerkship program) on the perceived performance of their technical and non-technical skills. In addition, we analyzed the difference between instructor's and learners' evaluations of technical skills after the simulation. A one-group pretest-posttest design was adopted. The simulation-based education of the neonatal resuscitation program (NRP) was conducted for 40 medical students from July to November 2020 at a medical school in South Korea. The simulation-based education comprised 5 minutes of pre-briefing, 10 minutes of running the simulation, and 30 minutes of debriefing (using a recorded video). The perceived performance of students' technical and non-technical skills before and after the simulation was compared by collecting and analyzing the pre- and post-questionnaires. The perceived performance of technical (p = .001) and non-technical skills (p < .001) was found to have significantly increased after the simulation. Particularly, the performance of technical skills, such as diagnostic (p = .007) and therapeutic actions (p < .001) and non-technical skills, such as leadership (p < .001), teamwork (p = .001), and task management (p = .020) improved significantly. There was no significant difference in the evaluations of the technical performance of the instructor and learners after the simulation (p = .953). Simulation-based education can improve technical skills, such as diagnostic and therapeutic actions for neonatal resuscitation. It is also effective in enhancing non-technical skills, such as leadership, teamwork, and task management. Further, after the simulation-based education, students can fully self-evaluate through objective reflection and improve their clinical competency.
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- 2022
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113. Genetic determinants of telomere length from 109,122 ancestrally diverse whole-genome sequences in TOPMed
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Margaret A. Taub, Matthew P. Conomos, Rebecca Keener, Kruthika R. Iyer, Joshua S. Weinstock, Lisa R. Yanek, John Lane, Tyne W. Miller-Fleming, Jennifer A. Brody, Laura M. Raffield, Caitlin P. McHugh, Deepti Jain, Stephanie M. Gogarten, Cecelia A. Laurie, Ali Keramati, Marios Arvanitis, Albert V. Smith, Benjamin Heavner, Lucas Barwick, Lewis C. Becker, Joshua C. Bis, John Blangero, Eugene R. Bleecker, Esteban G. Burchard, Juan C. Celedón, Yen Pei C. Chang, Brian Custer, Dawood Darbar, Lisa de las Fuentes, Dawn L. DeMeo, Barry I. Freedman, Melanie E. Garrett, Mark T. Gladwin, Susan R. Heckbert, Bertha A. Hidalgo, Marguerite R. Irvin, Talat Islam, W. Craig Johnson, Stefan Kaab, Lenore Launer, Jiwon Lee, Simin Liu, Arden Moscati, Kari E. North, Patricia A. Peyser, Nicholas Rafaels, Christine Seidman, Daniel E. Weeks, Fayun Wen, Marsha M. Wheeler, L. Keoki Williams, Ivana V. Yang, Wei Zhao, Stella Aslibekyan, Paul L. Auer, Donald W. Bowden, Brian E. Cade, Zhanghua Chen, Michael H. Cho, L. Adrienne Cupples, Joanne E. Curran, Michelle Daya, Ranjan Deka, Celeste Eng, Tasha E. Fingerlin, Xiuqing Guo, Lifang Hou, Shih-Jen Hwang, Jill M. Johnsen, Eimear E. Kenny, Albert M. Levin, Chunyu Liu, Ryan L. Minster, Take Naseri, Mehdi Nouraie, Muagututi‘a Sefuiva Reupena, Ester C. Sabino, Jennifer A. Smith, Nicholas L. Smith, Jessica Lasky-Su, James G. Taylor, VI, Marilyn J. Telen, Hemant K. Tiwari, Russell P. Tracy, Marquitta J. White, Yingze Zhang, Kerri L. Wiggins, Scott T. Weiss, Ramachandran S. Vasan, Kent D. Taylor, Moritz F. Sinner, Edwin K. Silverman, M. Benjamin Shoemaker, Wayne H.-H. Sheu, Frank Sciurba, David A. Schwartz, Jerome I. Rotter, Daniel Roden, Susan Redline, Benjamin A. Raby, Bruce M. Psaty, Juan M. Peralta, Nicholette D. Palmer, Sergei Nekhai, Courtney G. Montgomery, Braxton D. Mitchell, Deborah A. Meyers, Stephen T. McGarvey, Angel C.Y. Mak, Ruth J.F. Loos, Rajesh Kumar, Charles Kooperberg, Barbara A. Konkle, Shannon Kelly, Sharon L.R. Kardia, Robert Kaplan, Jiang He, Hongsheng Gui, Frank D. Gilliland, Bruce D. Gelb, Myriam Fornage, Patrick T. Ellinor, Mariza de Andrade, Adolfo Correa, Yii-Der Ida Chen, Eric Boerwinkle, Kathleen C. Barnes, Allison E. Ashley-Koch, Donna K. Arnett, Christine Albert, Cathy C. Laurie, Goncalo Abecasis, Deborah A. Nickerson, James G. Wilson, Stephen S. Rich, Daniel Levy, Ingo Ruczinski, Abraham Aviv, Thomas W. Blackwell, Timothy Thornton, Jeff O’Connell, Nancy J. Cox, James A. Perry, Mary Armanios, Alexis Battle, Nathan Pankratz, Alexander P. Reiner, and Rasika A. Mathias
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telomeres ,telomere length genetics ,trans-population genome-wide association study ,Genetics ,QH426-470 ,Internal medicine ,RC31-1245 - Abstract
Summary: Genetic studies on telomere length are important for understanding age-related diseases. Prior GWASs for leukocyte TL have been limited to European and Asian populations. Here, we report the first sequencing-based association study for TL across ancestrally diverse individuals (European, African, Asian, and Hispanic/Latino) from the NHLBI Trans-Omics for Precision Medicine (TOPMed) program. We used whole-genome sequencing (WGS) of whole blood for variant genotype calling and the bioinformatic estimation of telomere length in n = 109,122 individuals. We identified 59 sentinel variants (p < 5 × 10−9) in 36 loci associated with telomere length, including 20 newly associated loci (13 were replicated in external datasets). There was little evidence of effect size heterogeneity across populations. Fine-mapping at OBFC1 indicated that the independent signals colocalized with cell-type-specific eQTLs for OBFC1 (STN1). Using a multi-variant gene-based approach, we identified two genes newly implicated in telomere length, DCLRE1B (SNM1B) and PARN. In PheWAS, we demonstrated that our TL polygenic trait scores (PTSs) were associated with an increased risk of cancer-related phenotypes.
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- 2022
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114. Expression-Based Cell Lineage Analysis in Drosophila Through a Course-Based Research Experience for Early Undergraduates
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John M. Olson, Cory J. Evans, Kathy T. Ngo, Hee Jong Kim, Joseph Duy Nguyen, Kayla G. H. Gurley, Truc Ta, Vijay Patel, Lisa Han, Khoa T. Truong-N, Letty Liang, Maggie K. Chu, Hiu Lam, Hannah G. Ahn, Abhik Kumar Banerjee, In Young Choi, Ross G. Kelley, Naseem Moridzadeh, Awais M. Khan, Omair Khan, Szuyao Lee, Elizabeth B. Johnson, Annie Tigranyan, Jay Wang, Anand D. Gandhi, Manish M. Padhiar, Joseph Hargan Calvopina, Kirandeep Sumra, Kristy Ou, Jessie C. Wu, Joseph N. Dickan, Sabrena M. Ahmadi, Donald N. Allen, Van Thanh Mai, Saif Ansari, George Yeh, Earl Yoon, Kimberly Gon, John Y. Yu, Johnny He, Jesse M. Zaretsky, Noemi E. Lee, Edward Kuoy, Alexander N. Patananan, Daniel Sitz, PhuongThao Tran, Minh-Tu Do, Samira J. Akhave, Silverio D. Alvarez, Bobby Asem, Neda Asem, Nicole A. Azarian, Arezou Babaesfahani, Ahmad Bahrami, Manjeet Bhamra, Ragini Bhargava, Rakesh Bhatia, Subir Bhatia, Nicholas Bumacod, Jonathan J. Caine, Thomas A. Caldwell, Nicole A. Calica, Elise M. Calonico, Carman Chan, Helen H.-L. Chan, Albert Chang, Chiaen Chang, Daniel Chang, Jennifer S. Chang, Nauman Charania, Jasmine Y. Chen, Kevin Chen, Lu Chen, Yuyu Chen, Derek J. Cheung, Jesse J. Cheung, Jessica J. Chew, Nicole B. Chew, Cheng-An Tony Chien, Alana M. Chin, Chee Jia Chin, Youngho Cho, Man Ting Chou, Ke-Huan K. Chow, Carolyn Chu, Derrick M. Chu, Virginia Chu, Katherine Chuang, Arunit Singh Chugh, Mark R. Cubberly, Michael Guillermo Daniel, Sangita Datta, Raj Dhaliwal, Jenny Dinh, Dhaval Dixit, Emmylou Dowling, Melinda Feng, Christopher M. From, Daisuke Furukawa, Himaja Gaddipati, Lilit Gevorgyan, Zunera Ghaznavi, Tulika Ghosh, Jaskaran Gill, David J. Groves, Kalkidan K. Gurara, Ali R. Haghighi, Alexandra L. Havard, Nasser Heyrani, Tanya Hioe, Kirim Hong, Justin J. Houman, Molly Howland, Elaine L. Hsia, Justin Hsueh, Stacy Hu, Andrew J. Huang, Jasmine C. Huynh, Jenny Huynh, Chris Iwuchukwu, Michael J. Jang, An An Jiang, Simran Kahlon, Pei-Yun Kao, Manpreet Kaur, Matthew G. Keehn, Elizabeth J. Kim, Hannah Kim, Michelle J. Kim, Shawn J. Kim, Aleksandar Kitich, Ross A. Kornberg, Nicholas G. Kouzelos, Jane Kuon, Bryan Lau, Roger K. Lau, Rona Law, Huy D. Le, Rachael Le, Carrou Lee, Christina Lee, Grace E. Lee, Kenny Lee, Michelle J. Lee, Regina V. Lee, Sean H. K. Lee, Sung Kyu Lee, Sung-Ling D. Lee, Yong Jun Lee, Megan J. Leong, David M. Li, Hao Li, Xingfu Liang, Eric Lin, Michelle M. Lin, Peter Lin, Tiffany Lin, Stacey Lu, Serena S. Luong, Jessica S. Ma, Li Ma, Justin N. Maghen, Sravya Mallam, Shivtaj Mann, Jason H. Melehani, Ryan C. Miller, Nitish Mittal, Carmel M. Moazez, Susie Moon, Rameen Moridzadeh, Kaley Ngo, Hanh H. Nguyen, Kambria Nguyen, Thien H. Nguyen, Angela W. Nieh, Isabella Niu, Seo-Kyung Oh, Jessica R. Ong, Randi K. Oyama, Joseph Park, Yaelim A. Park, Kimberly A. Passmore, Ami Patel, Amy A. Patel, Dhruv Patel, Tirth Patel, Katherine E. Peterson, An Huynh Pham, Steven V. Pham, Melissa E. Phuphanich, Neil D. Poria, Alexandra Pourzia, Victoria Ragland, Riki D. Ranat, Cameron M. Rice, David Roh, Solomon Rojhani, Lili Sadri, Agafe Saguros, Zainab Saifee, Manjot Sandhu, Brooke Scruggs, Lisa M. Scully, Vanessa Shih, Brian A. Shin, Tamir Sholklapper, Harnek Singh, Sumedha Singh, Sondra L. Snyder, Katelyn F. Sobotka, Sae Ho Song, Siddharth Sukumar, Halley C. Sullivan, Mark Sy, Hande Tan, Sara K. Taylor, Shivani K. Thaker, Tulsi Thakore, Gregory E. Tong, Jacinda N. Tran, Jonathan Tran, Tuan D. Tran, Vivi Tran, Cindy L. Trang, Hung G. Trinh, Peter Trinh, Han-Ching H. Tseng, Ted T. Uotani, Akram V. Uraizee, Kent K. T. Vu, Kevin K. T. Vu, Komal Wadhwani, Paluk K. Walia, Rebecca S. Wang, Shuo Wang, Stephanie J. Wang, Danica D. Wiredja, Andrew L. Wong, Daniel Wu, Xi Xue, Griselda Yanez, Yung-Hsuan Yang, Zhong Ye, Victor W. Yee, Cynthia Yeh, Yue Zhao, Xin Zheng, Anke Ziegenbalg, Jon Alkali, Ida Azizkhanian, Akash Bhakta, Luke Berry, Ryen Castillo, Sonja Darwish, Holly Dickinson, Ritika Dutta, Rahul Kumar Ghosh, Riley Guerin, Jonathan Hofman, Garrick Iwamoto, Sarah Kang, Andrew Kim, Brian Kim, Hanwool Kim, Kristine Kim, Suji Kim, Julie Ko, Michael Koenig, Alejandro LaRiviere, Clifton Lee, Jiwon Lee, Brandon Lung, Max Mittelman, Mark Murata, Yujin Park, Daniel Rothberg, Ben Sprung-Keyser, Kunal Thaker, Vivian Yip, Paul Picard, Francie Diep, Nikki Villarasa, Volker Hartenstein, Casey Shapiro, Marc Levis-Fitzgerald, Leslie Jaworski, David Loppato, Ira E. Clark, and Utpal Banerjee
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g-trace ,gene expression ,education ,stem ,cure ,Genetics ,QH426-470 - Abstract
A variety of genetic techniques have been devised to determine cell lineage relationships during tissue development. Some of these systems monitor cell lineages spatially and/or temporally without regard to gene expression by the cells, whereas others correlate gene expression with the lineage under study. The GAL4 Technique for Real-time and Clonal Expression (G-TRACE) system allows for rapid, fluorescent protein-based visualization of both current and past GAL4 expression patterns and is therefore amenable to genome-wide expression-based lineage screens. Here we describe the results from such a screen, performed by undergraduate students of the University of California, Los Angeles (UCLA) Undergraduate Research Consortium for Functional Genomics (URCFG) and high school summer scholars as part of a discovery-based education program. The results of the screen, which reveal novel expression-based lineage patterns within the brain, the imaginal disc epithelia, and the hematopoietic lymph gland, have been compiled into the G-TRACE Expression Database (GED), an online resource for use by the Drosophila research community. The impact of this discovery-based research experience on student learning gains was assessed independently and shown to be greater than that of similar programs conducted elsewhere. Furthermore, students participating in the URCFG showed considerably higher STEM retention rates than UCLA STEM students that did not participate in the URCFG, as well as STEM students nationwide.
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- 2019
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115. Multi-ancestry sleep-by-SNP interaction analysis in 126,926 individuals reveals lipid loci stratified by sleep duration
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Raymond Noordam, Maxime M. Bos, Heming Wang, Thomas W. Winkler, Amy R. Bentley, Tuomas O. Kilpeläinen, Paul S. de Vries, Yun Ju Sung, Karen Schwander, Brian E. Cade, Alisa Manning, Hugues Aschard, Michael R. Brown, Han Chen, Nora Franceschini, Solomon K. Musani, Melissa Richard, Dina Vojinovic, Stella Aslibekyan, Traci M. Bartz, Lisa de las Fuentes, Mary Feitosa, Andrea R. Horimoto, Marjan Ilkov, Minjung Kho, Aldi Kraja, Changwei Li, Elise Lim, Yongmei Liu, Dennis O. Mook-Kanamori, Tuomo Rankinen, Salman M. Tajuddin, Ashley van der Spek, Zhe Wang, Jonathan Marten, Vincent Laville, Maris Alver, Evangelos Evangelou, Maria E. Graff, Meian He, Brigitte Kühnel, Leo-Pekka Lyytikäinen, Pedro Marques-Vidal, Ilja M. Nolte, Nicholette D. Palmer, Rainer Rauramaa, Xiao-Ou Shu, Harold Snieder, Stefan Weiss, Wanqing Wen, Lisa R. Yanek, Correa Adolfo, Christie Ballantyne, Larry Bielak, Nienke R. Biermasz, Eric Boerwinkle, Niki Dimou, Gudny Eiriksdottir, Chuan Gao, Sina A. Gharib, Daniel J. Gottlieb, José Haba-Rubio, Tamara B. Harris, Sami Heikkinen, Raphaël Heinzer, James E. Hixson, Georg Homuth, M. Arfan Ikram, Pirjo Komulainen, Jose E. Krieger, Jiwon Lee, Jingmin Liu, Kurt K. Lohman, Annemarie I. Luik, Reedik Mägi, Lisa W. Martin, Thomas Meitinger, Andres Metspalu, Yuri Milaneschi, Mike A. Nalls, Jeff O’Connell, Annette Peters, Patricia Peyser, Olli T. Raitakari, Alex P. Reiner, Patrick C. N. Rensen, Treva K. Rice, Stephen S. Rich, Till Roenneberg, Jerome I. Rotter, Pamela J. Schreiner, James Shikany, Stephen S. Sidney, Mario Sims, Colleen M. Sitlani, Tamar Sofer, Konstantin Strauch, Morris A. Swertz, Kent D. Taylor, André G. Uitterlinden, Cornelia M. van Duijn, Henry Völzke, Melanie Waldenberger, Robert B. Wallance, Ko Willems van Dijk, Caizheng Yu, Alan B. Zonderman, Diane M. Becker, Paul Elliott, Tõnu Esko, Christian Gieger, Hans J. Grabe, Timo A. Lakka, Terho Lehtimäki, Kari E. North, Brenda W. J. H. Penninx, Peter Vollenweider, Lynne E. Wagenknecht, Tangchun Wu, Yong-Bing Xiang, Wei Zheng, Donna K. Arnett, Claude Bouchard, Michele K. Evans, Vilmundur Gudnason, Sharon Kardia, Tanika N. Kelly, Stephen B. Kritchevsky, Ruth J. F. Loos, Alexandre C. Pereira, Mike Province, Bruce M. Psaty, Charles Rotimi, Xiaofeng Zhu, Najaf Amin, L. Adrienne Cupples, Myriam Fornage, Ervin F. Fox, Xiuqing Guo, W. James Gauderman, Kenneth Rice, Charles Kooperberg, Patricia B. Munroe, Ching-Ti Liu, Alanna C. Morrison, Dabeeru C. Rao, Diana van Heemst, and Susan Redline
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Science - Abstract
Sleep duration is associated with an adverse lipid profile. Here, the authors perform genome-wide gene-by-sleep interaction analysis and find 49 previously unreported lipid loci when considering short or long total sleep time.
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- 2019
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116. An engineered human Fc domain that behaves like a pH-toggle switch for ultra-long circulation persistence
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Chang-Han Lee, Tae Hyun Kang, Ophélie Godon, Makiko Watanabe, George Delidakis, Caitlin M. Gillis, Delphine Sterlin, David Hardy, Michel Cogné, Lynn E. Macdonald, Andrew J. Murphy, Naxin Tu, Jiwon Lee, Jonathan R. McDaniel, Emily Makowski, Peter M. Tessier, Aaron S. Meyer, Pierre Bruhns, and George Georgiou
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Science - Abstract
Lee et al. report an engineered IgG1 Fc domain that behaves like an hFcRn binding pH toggle switch. The authors show that this new half-life extension Fc domain confers improved pharmacokinetics in new humanized knock-in mouse strains that recapitulate the key processes for antibody persistence in circulation.
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- 2019
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117. Novel Small Molecules Capable of Blocking mtRAS-Signaling Pathway
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Namkyoung Kim, Injae Shin, Younghoon Kim, Eunhye Jeon, Jiwon Lee, Chaeyoung Lee, Yunju Nam, Sumin Lee, Eunhye Ju, Chan Kim, Woolim Son, SeongShick Ryu, Minjoo Ko, and Taebo Sim
- Subjects
RAS signaling pathway ,cancers with mtRAS ,mutated RAS ,RAS signaling blocker ,multi-targeted kinase inhibitor ,GNF-7 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
RAS mutants are involved in approximately 30% of all human cancers and have been regarded as undruggable targets owing to relatively smooth protein surface and obscure binding pockets. In our previous study, we have demonstrated that GNF-7, a multi-targeted kinase inhibitor, possesses potent anti-proliferative activity against Ba/F3 cells transformed with NRAS-G12D. Based on our further analysis using Ba/F3 cells transformed with mtRAS, we discovered a series of pyrimido[4,5-d]pyrimidin-2-one analogues as mtRAS-signaling pathway blockers. In addition, our efforts expanded the assessment to cancer cells with mtRAS, which revealed that these substances are also capable of strongly suppressing the proliferation of various cancer cells harboring KRAS-G12D (AsPC-1), KRAS-G12V (SW480, DU-145), KRAS-G12C (H358), KRAS-G13D (MDA-MB-231), KRAS-Q61L (HT-29), and NRAS-Q61L (OCI-AML3). We herein report novel and potent mtRAS-signaling pathway blockers, SIJ1795 and SIJ1772, possessing 2 to 10-fold increased anti-proliferative activities compared to those of GNF-7 on cancer cells harboring mtRAS as well as on Ba/F3 cells transformed with mtRAS. Both SIJ1795 and SIJ1772 attenuate phosphorylation of RAS downstream molecules (AKT and MEK) and induce apoptosis and G0/G1 cell cycle arrest on cancer cells with mtRAS. Moreover, both substances substantially suppress the migration, invasion, and colony formation of cancer cells harboring mtRAS. Taken together, this study led us to identification of SIJ1795 and SIJ1772 capable of strongly inhibiting mtRAS-signaling pathway on cancer cells harboring mtRAS.
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- 2021
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118. A Combination of Rosa Multiflora and Zizyphus Jujuba Enhance Sleep Quality in Anesthesia-Induced Mice
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Sanung Eom, Shinhui Lee, Jiwon Lee, Sung-Oh Sohn, Junho H. Lee, and Jaeman Park
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NCP—Rosa multiflora Thunb. (Yeongsil)/Zizyphus jujuba Miller (Sanjoin) natural combination product ,sleep ,Traditional Korean Medicine ,melatonin receptor ,Rosa multiflora Thunb. (Yeongsil) ,SCN—suprachiasmatic nucleus ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Sleep is an essential component of quality of life. The majority of people experience sleep problems that impact their quality of life. Melatonin is currently a representative sleep aid. However, it is classified as a prescription drug in most countries, and consumers cannot purchase it to improve their sleep. This sleep induction experiment in mice aimed to identify a natural combination product (NCP) that can create synergistic sleep-promoting effects. Based on the mechanism of action of sleep, we investigated whether phenomenological indicators of sleep quality change according to the intake of NCP. The sleep onset and sleep time of the mice that consumed the NCP found by this study were improved compared to the existing sleep aids. The mean melatonin level in the blood increased by 197% compared to the control. To our knowledge, this is the first study to demonstrate that Rosa multiflora Thunb. (Yeongsil) can promote sleep similarly to Zizyphus jujuba Miller (Sanjoin). The results indicate a preclinical study of NCPs containing Rosa multiflora Thunb and Zizyphus jujuba Miller developed by us showed significant differences in sleep incubation and duration depending on melatonin concentrations. Our results also suggest that increased melatonin concentrations in the blood are likely to improve sleep quality, especially regarding incubation periods.
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- 2022
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119. A 0.78 mW Low-Power 4.02 High-Compression Ratio Less than 10-6 BER Error-Tolerant Lossless Image Compression Hardware for Wireless Capsule Endoscopy System.
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Jiwon Lee, Kyoung-Rog Lee, and Hoi-Jun Yoo
- Published
- 2018
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120. Combinatorial subset difference public key broadcast encryption scheme for secure multicast.
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Jihye Kim, Seunghwa Lee, Jiwon Lee, and Hyunok Oh
- Published
- 2018
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121. Scalable Wildcarded Identity-Based Encryption.
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Jihye Kim, Seunghwa Lee, Jiwon Lee, and Hyunok Oh
- Published
- 2018
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122. A 0.5V 9.26μW 15.28mΩ/√Hz Bio-Impedance Sensor IC With 0.55° Overall Phase Error.
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Kwantae Kim, Ji-Hoon Kim, Surin Gweon, Jiwon Lee, Minseo Kim, Yongsu Lee, Soyeon Kim, and Hoi-Jun Yoo
- Published
- 2019
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123. Draft Genome Sequence of the White-Rot Fungus Schizophyllum Commune IUM1114-SS01
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Da-Woon Kim, Junhyeok Nam, Ha Thi Kim Nguyen, Jiwon Lee, Yongjun Choi, and Jaehyuk Choi
- Subjects
schizophyllum commune ,dye decolorization ,whole genome sequence ,Botany ,QK1-989 - Abstract
The monokaryotic strain, Schizophyllum commune strain IUM1114-SS01, was generated from a basidiospore of dikaryotic parental strain IUM1114. It even showed the decolorizing activities for several textile dyes much better than its parental strain. Based on the results of a single-molecule real-time sequencing technology, we present the draft genome of S. commune IUM1114-SS01, comprising 41.1 Mb with GC contents of the genome were 57.44%. Among 13,380 protein-coding genes, 534 genes are carbon hydrate-active enzyme coding genes.
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- 2021
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124. Investigation of the Global Shutter Operation of the Quantum-Dot Short-Wave Infrared Image Sensor
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Jaenam Kim, Joo-Hyoung Kim, Yun-Tzu Chang, Jihoon Park, Minhyun Jin, Vladimir Pejović, Epimitheas Georgitzikis, Steven Thijs, Itai Lieberman, Yunlong Li, Paul Heremans, Paweł Malinowski, Jung-Hoon Chun, and Jiwon Lee
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Electrical and Electronic Engineering ,Electronic, Optical and Magnetic Materials - Published
- 2023
125. DDX3 Upregulates Hydrogen Peroxide-Induced Melanogenesis in Sk-Mel-2 Human Melanoma Cells
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Sanung Eom, Shinhui Lee, Jiwon Lee, Hye Duck Yeom, Seong-Gene Lee, and Junho Lee
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DDX3 ,hydroperoxide ,melanogenesis ,SK-Mel-2 human cancer melanoma cell ,Organic chemistry ,QD241-441 - Abstract
DDX3 is a DEAD-box RNA helicase with diverse biological functions through multicellular pathways. The objective of this study was to investigate the role of DDX3 in regulating melanogenesis by the exploring signaling pathways involved. Various concentrations of hydrogen peroxide were used to induce melanogenesis in SK-Mel-2 human melanoma cells. Melanin content assays, tyrosinase activity analysis, and Western blot analysis were performed to determine how DDX3 was involved in melanogenesis. Transient transfection was performed to overexpress or silence DDX3 genes. Immunoprecipitation was performed using an antityrosinase antibody. Based on the results of the cell viability test, melanin content, and activity of tyrosinase, a key melanogenesis enzyme, in SK-Mel-2 human melanoma cells, hydrogen peroxide at 0.1 mM was chosen to induce melanogenesis. Treatment with H2O2 notably increased the promoter activity of DDX3. After treatment with hydroperoxide for 4 h, melanin content and tyrosinase activity peaked in DDX3-transfected cells. Overexpression of DDX3 increased melanin content and tyrosinase expression under oxidative stress induced by H2O2. DDX3 co-immunoprecipitated with tyrosinase, a melanogenesis enzyme. The interaction between DDX3 and tyrosinase was strongly increased under oxidative stress. DDX3 could increase melanogenesis under the H2O2-treated condition. Thus, targeting DDX3 could be a novel strategy to develop molecular therapy for skin diseases.
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- 2022
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126. A New Method of Myostatin Inhibition in Mice via Oral Administration of Lactobacillus casei Expressing Modified Myostatin Protein, BLS-M22
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Dong Kyung Sung, Hyeongseop Kim, Sang Eon Park, Jiwon Lee, Ju-A Kim, Young-Chul Park, Hong Bae Jeon, Jong Wook Chang, and Jeehun Lee
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myostatin ,Lactobacillus casei ,poly-gamma-glutamic acid synthetase A ,Duchenne muscular dystrophy ,mdx mouse ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Myostatin is a member of the transforming growth factor-beta superfamily and is an endogenous negative regulator of muscle growth. This study aimed to determine whether an oral administration of Lactobacillus casei expressing modified human myostatin (BLS-M22) could elicit sufficient levels of myostatin-specific antibody and improve the dystrophic features of an animal model of Duchenne muscular dystrophy (DMD; mdx mouse). BLS-M22 is a recombinant L. casei engineered to harbor the pKV vector and poly-gamma-glutamic acid gene linked to a modified human myostatin gene. Serological analysis showed that anti-myostatin IgG titers were significantly increased, and serum creatine kinase was significantly reduced in the BLS-M22-treated mdx mice compared to the control mice. In addition, treatment of BLS-M22 resulted in a significant increase in body weight and motor function (Rotarod behavior test). Histological analysis showed an improvement in the dystrophic features (fibrosis and muscle hypertrophy) of the mdx mice with the administration of BLS-M22. The circulating antibodies generated after BLS-M22 oral administration successfully lowered serum myostatin concentration. Myostatin blockade resulted in serological, histological, and functional improvements in mdx mice. Overall, the findings suggest the potential of BLS-M22 to treat DMD; however, further clinical trials are essential to ascertain its efficacy and safety in humans.
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- 2022
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127. Effect of group type on group performance in peer-collaborated two-round physics problem solving
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Jiwon Lee and Nilüfer Didiş Körhasan
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Special aspects of education ,LC8-6691 ,Physics ,QC1-999 - Abstract
Peers play an important role in an individual’s learning via collaboration. This study examined three semesters worth of student data collected through collaborative exams in a calculus-based introductory physics course. Participants were asked 142 physics problems over two rounds, and they answered the problems first individually (first round) and then with their groups (second round). Based on the group type determined by the number of correct answers, a majority of correct or incorrect answers, and a variety of answers in the individual round, we examined how group type affects group performance in the group round. A majority of correct and incorrect answers and the variety of answers in the individual round were important for group-round performance. The percentage of finding the correct answer in the group round was directly proportional to the number of students with correct responses in the individual round. Furthermore, an increase in the number of correct answers in the individual round increased the group performance when similar conditions were seen in the majority of correct and incorrect answers and the variety of answers in the previous round.
- Published
- 2022
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128. Dynamics of ex post uncertainty and negative behavioural direction in alliances.
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Inwon Kang and Jiwon Lee
- Published
- 2019
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129. A Four-Camera VGA-Resolution Capsule Endoscope System With 80-Mb/s Body Channel Communication Transceiver and Sub-Centimeter Range Capsule Localization.
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Jaeeun Jang, Jihee Lee, Kyoung-Rog Lee, Jiwon Lee, Minseo Kim, Yongsu Lee, Joonsung Bae, and Hoi-Jun Yoo
- Published
- 2019
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130. AuthCropper: Authenticated Image Cropper for Privacy Preserving Surveillance Systems.
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Jihye Kim, Jiwon Lee, Hankyung Ko, Donghwan Oh, Semin Han, Gwonho Jeong, and Hyunok Oh
- Published
- 2019
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131. BinomiRare: A robust test for association of a rare genetic variant with a binary outcome for mixed models and any case-control proportion
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Tamar Sofer, Jiwon Lee, Nuzulul Kurniansyah, Deepti Jain, Cecelia A. Laurie, Stephanie M. Gogarten, Matthew P. Conomos, Ben Heavner, Yao Hu, Charles Kooperberg, Jeffrey Haessler, Ramachandran S. Vasan, L. Adrienne Cupples, Brandon J. Coombes, Amanda Seyerle, Sina A. Gharib, Han Chen, Jeffrey R. O’Connell, Man Zhang, Daniel J. Gottlieb, Bruce M. Psaty, W.T. Longstreth, Jr., Jerome I. Rotter, Kent D. Taylor, Stephen S. Rich, Xiuqing Guo, Eric Boerwinkle, Alanna C. Morrison, James S. Pankow, Andrew D. Johnson, Nathan Pankratz, Alex P. Reiner, Susan Redline, Nicholas L. Smith, Kenneth M. Rice, and Elizabeth D. Schifano
- Subjects
genome sequencing ,rare variant assocaition testing ,diverse populations ,targeted gene analysis ,mixed models ,Genetics ,QH426-470 - Abstract
Summary: Whole-genome sequencing (WGS) and whole-exome sequencing studies have become increasingly available and are being used to identify rare genetic variants associated with health and disease outcomes. Investigators routinely use mixed models to account for genetic relatedness or other clustering variables (e.g., family or household) when testing genetic associations. However, no existing tests of the association of a rare variant with a binary outcome in the presence of correlated data control the type 1 error where there are (1) few individuals harboring the rare allele, (2) a small proportion of cases relative to controls, and (3) covariates to adjust for. Here, we address all three issues in developing a framework for testing rare variant association with a binary trait in individuals harboring at least one risk allele. In this framework, we estimate outcome probabilities under the null hypothesis and then use them, within the individuals with at least one risk allele, to test variant associations. We extend the BinomiRare test, which was previously proposed for independent observations, and develop the Conway-Maxwell-Poisson (CMP) test and study their properties in simulations. We show that the BinomiRare test always controls the type 1 error, while the CMP test sometimes does not. We then use the BinomiRare test to test the association of rare genetic variants in target genes with small-vessel disease (SVD) stroke, short sleep, and venous thromboembolism (VTE), in whole-genome sequence data from the Trans-Omics for Precision Medicine (TOPMed) program.
- Published
- 2021
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132. A Prevalent Focused Human Antibody Response to the Influenza Virus Hemagglutinin Head Interface
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Kevin R. McCarthy, Jiwon Lee, Akiko Watanabe, Masayuki Kuraoka, Lindsey R. Robinson-McCarthy, George Georgiou, Garnett Kelsoe, and Stephen C. Harrison
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Microbiology ,QR1-502 - Abstract
The rapid appearance of mutations in circulating human influenza viruses and selection for escape from herd immunity require prediction of likely variants for an annual updating of influenza vaccines. The identification of human antibodies that recognize conserved surfaces on the influenza virus hemagglutinin (HA) has prompted efforts to design immunogens that might selectively elicit such antibodies.
- Published
- 2021
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133. Establishing Conditions for Blood Smear Drying and Staining on Sysmex SP-50 for Leukocyte Differential Count.
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Hyun Lee, Jihye Kim, Jiwon Lee, Jisoo Won, and Yoon Hwan Chang
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LEUKOCYTE count ,BLOODSTAINS ,BASOPHILS ,EOSINOPHILS ,LYMPHOCYTES ,MONOCYTES - Abstract
Background: The purpose of this study was to determine the staining conditions and appropriate fan1 start time (FAN1ST) for Sysmex SP-50 to produce blood smears (BS) that reflect the true lymphocyte morphology of patient samples. Methods: Using different start times of fan1, we obtained a set of 84 blood smear slides from 21 blood samples and measured 10,920 lymphocyte areas, which were then converted to compare lymphocyte sizes. We also performed a leukocyte differential count using Sysmex DI-60 on 202 blood smear slides prepared before and after the change in staining conditions and compared the results. Results: The mean lymphocyte sizes at FAN1ST 0 second, 5 seconds, 10 seconds, and 30 seconds were 12.55 µm, 12.14 µm, 11.27 µm, and 10.50 µm, respectively. The mean differences in the pre-classification of neutrophils, lymphocytes, monocytes, eosinophils, and basophils in DI-60, according to the SP-50 staining conditions, were 0.88, -1.58, -0.24, 0.37, and 0.07, respectively. Conclusions: Wright-Giemsa staining of blood smears prepared on the SP-50 showed that changing the pH of the concentrated phosphate buffer to 6.6 and adjusting the staining time did not affect the results of the leukocyte differential count. However, since fan1 was used to dry the blood smear on the SP-50 and the lymphocyte size gradually decreased as the start time was delayed, it was necessary to set a start time for fan1 that did not affect the lymphocyte size. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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134. Optimal Insertion Depth of Central Venous Catheter through the Right Internal Jugular Vein, Verified by Transesophageal Echocardiography: A Prospective Observational Study.
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Jiwon Lee, Ji-Hoon Park, Sohee Oh, and Jung-Man Lee
- Published
- 2024
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135. Effect of neck extension on the advancement of tracheal tubes from the nasal cavity to the oropharynx in nasotracheal intubation: a randomized controlled trial
- Author
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Hyerim Kim, Jung-Man Lee, Jiwon Lee, Jin-Young Hwang, Jee-Eun Chang, Hyun-Joung No, Dongwook Won, Hyung Sang Row, and Seong-Won Min
- Subjects
Intubation, Nasotracheal ,Neck extension ,Tracheal tube ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Background Clinicians sometimes encounter resistance in advancing a tracheal tube, which is inserted via a nostril, from the nasal cavity into the oropharynx during nasotracheal intubation. The purpose of this study was to investigate the effect of neck extension on the advancement of tracheal tubes from the nasal cavity into the oropharynx during nasotracheal intubation. Methods Patients were randomized to the ‘neck extension group (E group)’ or ‘neutral position group (N group)’ for this randomized controlled trial. After induction of anesthesia, a nasal RAE tube was inserted via a nostril. For the E group, an anesthesiologist advanced the tube from the nasal cavity into the oropharynx with the patient’s neck extended. For the N group, an anesthesiologist advanced the tube without neck extension. If the tube was successfully advanced into the oropharynx within two attempts by the same maneuver according to the assigned group, the case was defined as ‘success.’ We compared the success rate of tube advancement between the two groups. Results Thirty-two patients in the E group and 33 in the N group completed the trial. The success rate of tube passage during the first two attempts was significantly higher in the E group than in the N group (93.8% vs. 60.6%; odds ratio = 9.75, 95% CI = [1.98, 47.94], p = 0.002). Conclusion Neck extension during tube advancement from the nasal cavity to the oropharynx before laryngoscopy could be helpful in nasotracheal intubation. Trial registration ClinicalTrials.gov Identifier NCT03377114, registered on 13 December 2017.
- Published
- 2019
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136. Rethinking Generalization Performance of Surgical Phase Recognition with Expert-Generated Annotations.
- Author
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Seungbum Hong, Jiwon Lee, Bokyung Park, Ahmed A. Alwusaibie, Anwar H. Alfadhel, SungHyun Park, Woo Jin Hyung, and Min-Kook Choi
- Published
- 2021
137. The Application of the Neuroprotective and Potential Antioxidant Effect of Ergotamine Mediated by Targeting N-Methyl-D-Aspartate Receptors
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Shinhui Lee, Sanung Eom, Khoa V. A. Nguyen, Jiwon Lee, Youngseo Park, Hye Duck Yeom, and Junho H. Lee
- Subjects
NMDA ,N-methyl-D-aspartate ,ergotamine ,ergot alkaloid ,antioxidant ,neuronal disease ,Therapeutics. Pharmacology ,RM1-950 - Abstract
(1) Background: The N-methyl-D-aspartate receptors (NMDARs) mediate fast excitatory currents leading to depolarization. Postsynaptic NMDARs are ionotropic glutamate receptors that mediate excitatory glutamate or glycine signaling in the CNS and play a primary role in long-term potentiation, which is a major form of use-dependent synaptic plasticity. The overstimulation of NMDARs mediates excessive Ca2+ influx to postsynaptic neurons and facilitates more production of ROS, which induces neuronal apoptosis. (2) Methods: To confirm the induced inward currents by the coapplication of glutamate and ergotamine on NMDARs, a two-electrode voltage clamp (TEVC) was conducted. The ergotamine-mediated inhibitory effects of NR1a/NR2A subunits were explored among four different kinds of recombinant NMDA subunits. In silico docking modeling was performed to confirm the main binding site of ergotamine. (3) Results: The ergotamine-mediated inhibitory effect on the NR1a/NR2A subunits has concentration-dependent, reversible, and voltage-independent properties. The major binding sites were V169 of the NR1a subunit and N466 of the NR2A subunit. (4) Conclusion: Ergotamine effectively inhibited NR1a/NR2A subunit among the subtypes of NMDAR. This inhibition effect can prevent excessive Ca2+ influx, which prevents neuronal death.
- Published
- 2022
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138. Wastewater Treatment by Polymeric Microspheres: A Review
- Author
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Jiwon Lee and Rajkumar Patel
- Subjects
polymeric microsphere ,wastewater ,vinylic polymer ,natural polymer ,adsorption ,Organic chemistry ,QD241-441 - Abstract
This review addresses polymer microspheres used as adsorbent for wastewater treatment. The removal of various pollutants (including dyes, heavy metal ions, and organic pollutants) is a prominent issue, as they can cause severe health problems. Porous microspheres can provide large specific area and active sites for adsorption or photo degradation. Enhancement in performance is achieved by various modifications, such as the introduction of nanoparticles, magnetic particles, and ZIF-8. Some microspheres were synthesized from synthetic polymers such as vinylic polymer and polydopamine (PDA) through a facile fabrication process. Natural polymers (such as cellulose, alginate, and chitosan) that are biodegradable and eco-friendly are also used. The adsorbents used in industrial application require high adsorption capacity, thermal stability, and recyclability. Batch adsorption experiments were conducted to investigate the optimal conditions, influence of related factors, and adsorption capacities. Insights regarding the adsorption mechanisms were given from the kinetic model, isotherm model, and various characterization methods. The recyclability is investigated through regeneration ratio, or their maintenance of their capability through repeated adsorption-desorption cycles. The high potential of polymer microsphere for the removal of pollutants from wastewater is shown through the high adsorption capacities, environmentally friendliness, and high stability.
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- 2022
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139. A splice variant of human Bmal1 acts as a negative regulator of the molecular circadian clock
- Author
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Jiwon Lee, Eonyoung Park, Ga Hye Kim, Ilmin Kwon, and Kyungjin Kim
- Subjects
Medicine ,Biochemistry ,QD415-436 - Abstract
Circadian rhythms: Alternative forms of clock protein have opposing effects An alternative form of a key ‘clock’ protein involved in the maintenance of daily cellular rhythms serves as a negative regulator of the cell’s 24-hour cycle. A team led by Ilmin Kwon from Sungkyunkwan University School of Medicine, Suwon, and Kyungjin Kim from Daegu Gyeongbuk Institute of Science and Technology, both in South Korea, detailed the function of BMAL1a, a lesser-studied variant of the clock protein BMAL1b, in human cells. Whereas BMAL1b enters the nucleus, where it works in concert with another protein called CLOCK to control circadian dynamics, BMAL1a stays in the cytoplasm, where it binds BMAL1b and CLOCK, interfering with their function. Genetically inhibiting BMAL1a helped restore normal rhythmic cycles. Drugs targeting BMAL1a may thus aid in sleep disorders and other circadian-linked health problems.
- Published
- 2018
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140. Exploring Objective and Subjective Correlates with the Vitality of Agro-Industrial Complex Companies in Korea
- Author
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Tae-Hyoung Tommy Gim, Jiwon Lee, and Jeong-seok Choi
- Subjects
Urban Studies ,Geography, Planning and Development ,Management, Monitoring, Policy and Law - Published
- 2023
141. A reference database of Windows artifacts for file‐wiping tool execution analysis
- Author
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Dabin Joo, Jiwon Lee, and Doowon Jeong
- Subjects
Genetics ,Pathology and Forensic Medicine - Published
- 2023
142. Towards Sustainable Campus Design Intervention -Focusing on Gap between Students and Cleaning Staff in University A Case
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Jiwon Lee, Yesul Lee, Jaeyoung Myung, Seung-Eun Lee, and Hyunjae Shin
- Subjects
Automotive Engineering - Published
- 2023
143. Pharmacological Interventions to Treat Antipsychotic-Induced Dyslipidemia in Schizophrenia Patients: A Systematic Review and Meta Analysis
- Author
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Pruntha Kanagasundaram, Jiwon Lee, Femin Prasad, Kenya A. Costa-Dookhan, Laurie Hamel, Madeleine Gordon, Gary Remington, Margaret K. Hahn, and Sri Mahavir Agarwal
- Subjects
schizophrenia ,antipsychotics ,dyslipidemia ,systematic review ,meta-analysis ,Psychiatry ,RC435-571 - Abstract
Introduction: Antipsychotic-induced dyslipidemia represents a common adverse effect faced by patients with schizophrenia that increases risk for developing further metabolic complications and cardiovascular disease. Despite its burden, antipsychotic-induced dyslipidemia is often left untreated, and the effectiveness of pharmacological interventions for mitigating dyslipidemia has not been well-addressed. This review aims to assess the effectiveness of pharmacological interventions in alleviating dyslipidemia in patients with schizophrenia.Methods: Medline, PsychInfo, and EMBASE were searched for all relevant English articles from 1950 to November 2020. Randomized placebo-controlled trials were included. Differences in changes in triglycerides, HDL cholesterol, LDL cholesterol, and VLDL cholesterol levels between treatment and placebo groups were meta-analyzed as primary outcomes.Results: Our review identified 48 randomized controlled trials that comprised a total of 3,128 patients and investigated 29 pharmacological interventions. Overall, pharmacological interventions were effective in lowering LDL cholesterol, triglycerides, and total cholesterol levels while increasing the levels of HDL cholesterol. Within the intervention subgroups, approved lipid-lowering agents did not reduce lipid parameters other than total cholesterol level, while antipsychotic switching and antipsychotic add-on interventions improved multiple lipid parameters, including triglycerides, LDL cholesterol, HDL cholesterol, and total cholesterol. Off label lipid lowering agents improved triglycerides and total cholesterol levels, with statistically significant changes seen with metformin.Conclusion: Currently available lipid lowering agents may not work as well in patients with schizophrenia who are being treated with antipsychotics. Additionally, antipsychotic switching, antipsychotic add-ons, and certain off label interventions might be more effective in improving some but not all associated lipid parameters. Future studies should explore novel interventions for effectively managing antipsychotic-induced dyslipidemia.Registration: PROSPERO 2020 CRD42020219982; https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020219982.
- Published
- 2021
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144. Influence of two-handed jaw thrust during tracheal intubation on postoperative sore throat: a prospective randomised study
- Author
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Hyub Huh, Doo Yeon Go, Jang Eun Cho, Jihoon Park, Jiwon Lee, and Hyun-Chang Kim
- Subjects
Medicine (General) ,R5-920 - Abstract
Objective General anaesthesia with tracheal intubation results in sore throat. We evaluated the influence of the two-handed jaw thrust on postoperative sore throat in patients who require tracheal intubation. Methods In this prospective, double-blind, single-centre, parallel-arm, and randomised trial, 92 patients who were scheduled for general anaesthesia for total hip arthroplasty were allocated to one of two groups. In the jaw thrust group (n = 46), the two-handed jaw thrust manoeuvre was applied at intubation. In the control group (n = 46), conventional intubation with sham jaw thrust was performed. Incidences of airway morbidities including sore throat, hoarseness, and cough at 2, 4, and 24 hours postoperatively were compared. Results During the postoperative 24 hours, the incidence of sore throat (8 [17%] vs. 20 [44%]) and hoarseness were lower in the jaw thrust group (8 [17%] vs. 18 [39%]) compared with the control group. The incidence of cough during the postoperative 24 hours was similar between the groups. Conclusions The jaw thrust manoeuvre significantly reduced sore throat and hoarseness in patients after general anaesthesia using tracheal intubation. Clinical trial registration: NCT 03568279.
- Published
- 2021
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145. A study on setting of the fatigue limit of temporary dental implants.
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Mi Hye Kim, Eunjeong Cho, Jiwon Lee, Eunkyo Kim, Sihyung Yoo, and Chang Won Park
- Published
- 2017
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146. An adaptive DC-balanced and multi-mode stimulator IC with 1GΩ output impedance for compact electro-acupuncture system.
- Author
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Jiwon Lee, Minseo Kim, Kwantae Kim, Kiseok Song, Sanghoon Lee, Weon Kim, Jong Shin Woo, and Hoi-Jun Yoo
- Published
- 2017
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147. Speech emotion recognition based on multi-task learning using a convolutional neural network.
- Author
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Nam Kyun Kim, Jiwon Lee, Hun Kyu Ha, Geon Woo Lee, Jung Hyuk Lee, and Hong Kook Kim
- Published
- 2017
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148. 4-Camera VGA-resolution capsule endoscope with 80Mb/s body-channel communication transceiver and Sub-cm range capsule localization.
- Author
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Jaeeun Jang, Jihee Lee, Kyoung-Rog Lee, Jiwon Lee, Minseo Kim, Yongsu Lee, Joonsung Bae, and Hoi-Jun Yoo
- Published
- 2018
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149. Distinct Features and Functions of Systemic and Mucosal Humoral Immunity Among SARS-CoV-2 Convalescent Individuals
- Author
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Savannah E. Butler, Andrew R. Crowley, Harini Natarajan, Shiwei Xu, Joshua A. Weiner, Carly A. Bobak, Daniel E. Mattox, Jiwon Lee, Wendy Wieland-Alter, Ruth I. Connor, Peter F. Wright, and Margaret E. Ackerman
- Subjects
antibody ,mucosal immunity ,IgA ,IgG ,neutralization ,SARS-CoV-2 ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Understanding humoral immune responses to SARS-CoV-2 infection will play a critical role in the development of vaccines and antibody-based interventions. We report systemic and mucosal antibody responses in convalescent individuals who experienced varying severity of disease. Whereas assessment of neutralization and antibody-mediated effector functions revealed polyfunctional antibody responses in serum, only robust neutralization and phagocytosis were apparent in nasal wash samples. Serum neutralization and effector functions correlated with systemic SARS-CoV-2-specific IgG response magnitude, while mucosal neutralization was associated with nasal SARS-CoV-2-specific IgA. Antibody depletion experiments support the mechanistic relevance of these correlations. Associations between nasal IgA responses, virus neutralization at the mucosa, and less severe disease suggest the importance of assessing mucosal immunity in larger natural infection cohorts. Further characterization of antibody responses at the portal of entry may define their ability to contribute to protection from infection or reduced risk of hospitalization, informing public health assessment strategies and vaccine development efforts.
- Published
- 2021
- Full Text
- View/download PDF
150. Genomic Analysis of Korean Patient With Microcephaly
- Author
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Jiwon Lee, Jong Eun Park, Chung Lee, Ah Reum Kim, Byung Joon Kim, Woong-Yang Park, Chang-Seok Ki, and Jeehun Lee
- Subjects
microcephaly ,whole exome sequencing (WES) ,chromosomal microarray ,low-depth whole genome sequencing ,Korea ,Genetics ,QH426-470 - Abstract
Microcephaly is a prevalent phenotype in patients with neurodevelopmental problems, often with genetic causes. We comprehensively investigated the clinical phenotypes and genetic background of microcephaly in 40 Korean patients. We analyzed their clinical phenotypes and radiologic images and conducted whole exome sequencing (WES) and analysis of copy number variation (CNV). Infantile hypotonia and developmental delay were present in all patients. Thirty-four patients (85%) showed primary microcephaly. The diagnostic yield from the WES and CNV analyses was 47.5%. With WES, we detected pathogenic or likely pathogenic variants that were previously associated with microcephaly in 12 patients (30%); nine of these were de novo variants with autosomal dominant inheritance. Two unrelated patients had mutations in the KMT2A gene. In 10 other patients, we found mutations in the GNB1, GNAO1, TCF4, ASXL1, SMC1A, VPS13B, ACTG1, EP300, and KMT2D genes. Seven patients (17.5%) were diagnosed with pathogenic CNVs. Korean patients with microcephaly show a genetic spectrum that is different from that of patients with microcephaly of other ethnicities. WES along with CNV analysis represents an effective approach for diagnosis of the underlying causes of microcephaly.
- Published
- 2021
- Full Text
- View/download PDF
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