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This study aimed to explore the effect of long non-coding RNA (LncRNA) LncOGD-1006 to ischemic stroke and the possible mechanism.The primary brain microvascular endothelial cells (bEnd.3) of oxygen-glucose deprivation (OGD) was used as a mimic of ischemic stroke in vitro.The results showed that LncOGD-1006 was upregulated in bEnd.3 after OGD-induced.LncOGD-1006 might act as a ceRNA to inhibit apoptosis in bEnd.3 cells by targeting miR-184-5p/CAAP1 pathway.

The focus on enhanced recovery after surgery (ERAS) is still the implementation of the concept at present, and remains in the stage of disordered and low-quality application because of its difficulties in operation, evaluation and repeatability. The main reason is lack of standard, feasible operation plan and process, which may lead to great heterogeneity and low quality. Therefore, It is urgent to explore and establish the quality control system of ERAS, including foundation, process, terminal and tracking quality control, so as to improve the validity, security, homogeneity and accessibility, and promote the further popularization and application. However, due to the conceptual, systematic, innovative and individual differences, the construction of ERAS standard quality control system will be faced with great difficulties and challenges. ERAS quality control platform and center can only be established gradually by hard work and a lot of researches.目前加速康复外科（ERAS）仍以贯彻理念为主，尚停留在"难以操作"、"难以评估"和"难以重复"的无序、低质应用阶段，其主要原因是缺乏标准、可操作的规范和流程，导致异质性问题突出，质量难以保证。因此，迫切需要探索和建立包括基础、过程、终末和跟踪质控在内的ERAS质量控制体系，以提高ERAS模式的有效性、安全性、同质性和可及性，促进ERAS的进一步推广和应用。但因ERAS的理念性、系统性、创新性和差异性等特点使构建ERAS标准质量控制体系将面临巨大的困难和挑战，需要艰苦的努力和大量的研究，才可能逐步建立ERAS质控平台、组建ERAS质控中心。.

Hepatocellular carcinoma (HCC) is a common malignant tumor. Increasing evidence has demonstrated that microRNAs (miRNAs) play an important role in a wide variety of cellular processes. However, there are few reports about the role and underlying molecular mechanisms of miRNAs in HCC.qRT-PCR and Western blots were performed to quantify the expression of miR-92a, E-cadherin, and circPTK2. Proliferation and invasion assays were performed to explore the function of miR-92a and circPTK2. A Luciferase assay was used to test the relationship between miR-92a, E-cadherin, and circPTK2.In this study, we found that miR-92a was upregulated in HCC tissues and HCC cell lines. Overexpression of miR-92a enhanced cell proliferation and invasion by targeting the E-cadherin 3'UTR in HCC cells. Furthermore, we found that circPTK2 inhibited EMT by inhibiting miR-92a, preventing its ability to downregulate E-cadherin in HCC cells.We identified a regulatory axis comprising circPTK2/miR-92a/E-cadherin in HCC cells that may serve as a valuable biomarker and therapeutic target for patients with HCC.

Acute lung injury (ALI) is one of the most serious complications of sepsis and remains refractory. It is of great significance to discuss the pathogenesis of acute lung injury in sepsis and look for more effective drugs for treatment. The purpose of this study was to investigate the role of LincRNA-p21 on acute lung injury in sepsis.Lung histology was detected by HE staining to evaluate sepsis-induced ALI model in rats. The miRNA expression of LincRNA-p21 in septic model in vivo and in vitro was detected by RT-qPCR. Cell apoptosis, inflammatory responses and oxidative stress were detected to uncover the influence of LincRNA-p21 on LPS-induced septic model in vitro.The expression of LincRNA-p21 was significantly increased in septic model in vivo and in vitro. Cell apoptosis, inflammatory responses and oxidative stress were alleviated by LincRNA-p21 interference in LPS-treated BEAS-2B cells.All the results in the current study proved that LincRNA-p21 interference could alleviate the acute lung injury in septic model. It raised the conclusion that LincRNA-p21 may act as a novel regulator in the pathological process and a potential therapeutic target in sepsis-induced ALI.

The 5G revolution have brought about the huge impact for the communication industry which produces a series of innovative technologies, such as Sub 6Ghz and millimmeter wave (mmWave) 28Ghz spectrum, beam forming, multiconnectivity and edge computing are widely applied in the 5G generation for high speed data transmission and low latency purpose. Furthermore, system in package (SiP) module design and assembly technology plays an key role in IOT, antenna in package (AiP) and RF module applicatons, compare to the tranditional SiP design, the Electromagnetic Interference (EMI) shielding technology is the important factor to influence the shielding effectiveness (SE). In this paper, the EMI coating and partition technology in SiP module will be discussed and analyzed in comformal and compartment shielding methold individually.In this study, Test Veihcle is designed with RF module with EMI shielding and partition technology. In the first part, the various sputter coating material and thickness are demonstrated by Near-Field Scanner meathold, compare to the conventional Cu coating layer, the Cu/Ferrite coating layer have improved about 10 dB SE at 6Ghz high frequency and 30 dB SE at 3MHz low frequency range sucessfully. In addition, the comparison between metal frame and epoxy paste partition shieldig capability resutls are discussed as well in this paper. Thus, the fillng material of metal frame, paste epoxy and SUS+Cu are demonstrated 40 dB SE improvement at 28GHz mmWave frequency by vector network analyzer (VNA) measurement. All of above physical phenomenon will be discussed in detail in the following sections.Finally, the reliability verification of EMI shielding on advanced SiP module will utilize standard JEDEC test condition including Temperature Cycle Test (TCT), High Temperature Storage (HTS), unbias-high accelerated stress test (u-HAST) results to verify the package structure. By employing this SiP structure with EMI shielding technology, the Shielding effectiveness (SE) are improved obviusly in both comformal and compartment structure. Undoubtedly, SiP with EMI shielding structure is absolutely the most innovative technology in the 5G generation.

Purpose: Following primary systemic treatment (PST), the optimal strategies for regional nodal irradiation (RNI) are currently under active investigation, especially in patients with pretreatment cN1 and posttreatment ypN0-1. The Neo-Bioscore staging system has showed promising prospect in assessing prognosis after PST. In this analysis, we evaluate the role of Neo-Bioscore staging system in guiding RNI following PST in patients with cN1 and ypN0-1. Methods and Materials: Continuous women with cN1 pretreatment and who received PST with ypN0 or ypN1 posttreatment between 2009 and 2014 were retrospectively reviewed. According to the report by Mittendorf et al, the Neo-Bioscore staging system is on the basis of pretreatment clinical stage, posttreatment pathologic stage, estrogen receptor (ER) status, HER2 status and grade stage, and assigned and summed points for each factor as shown in. A pathologic complete response (pCR) was defined as no invasive disease in the breast or regional lymph nodes after surgery. The curves for survival were generated using the Kaplan-Meier method and compared using the Log-rank test. Point Assignments for the Neo-Bioscore Staging SystemsFactorsPointsClinical stage I0IIA0IIB1IIIA1IIIB2IIIC2Pathologic stage 00I0IIA1IIB1IIIA1IIIB1IIIC2Tumor Biomarker ER negative1HER2 negative1Grade 31 Results: One hundred and sixty-three patients were enrolled in this study, of them 18 patients received breast conserving surgery. Of the 163 patients, 119 (73%) received RNI. At surgery, 36 patients (22.1%) achieved pCR, while 89 patients (54.6%) achieved ypN0. The median follow-up was 59.4 months (rang: 16-106). In the whole cohort, RNI was associated with non-significant improved outcomes, with a 5-year locoregional recurrence free survival (LRRFS) rate of 98.6% vs. 95.7% (P=0.393), a regional recurrence free survival (RRFS) rate of 98% vs. 97.7% (P=0.865), a 5-year distant metastasis free survival (DMFS) rate of 91.6% vs. 83.4% (P=0.052), a 5-year any first recurrence free survival (RFS) rate of 90.9% vs. 87.4% (P=0.43), and a 5-year overall survival (OS) rate of 98% vs. 91.9% (P=0.097) in the RNI and non-RNI group, respectively. In the subgroup of patients with Neo-Bioscore score of 1 to 3, RNI significantly increased the 5-year DMFS rate of 97% vs. 76.9% (P=0.002), 5-year RFS rate of 95.5% vs. 76.9% (P=0.007) and 5-year OS rate of 100% vs. 89.2% (P=0.005). However, no significant difference in outcomes was found between RNI and non-RNI group in patients with Neo-Bioscore score of 4 to 6. Among patients who not achieved pCR and those with ypN1, RNI significantly increased DMFS (both P Conclusions: Not all patients with cN1 and ypN0-1 could significantly benefit from RNI following PST, those with low Neo-Bioscore score were likely to benefit more. Citation Format: Cao L, Chen J-Y. The role of neo-bioscore staging system in guiding the optimal strategies for regional nodes irradiation following primary systemic treatment in breast cancer patients with cN1 and ypN0-1 [abstract]. In: Proceedings of the 2018 San Antonio Breast Cancer Symposium; 2018 Dec 4-8; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2019;79(4 Suppl):Abstract nr P3-12-08.

There are Chinese cherry and sweet cherry planted in Zhejiang Province, China. Chinese cherry (Prunus pseudocerasus L.) has been planted for many years. The industry is one of the smallest in the whole fruit industry in Zhejiang. The total acreage is 50,000 mu (=3333.3 ha), and the annual output is 13,000 t. But the average value mu(-1) which is about 20 to 30,000 Yuan is reasonably relatively high. The researches were concentrated on cultivars selection and orchard management. New selection ‘Zijing’ was selected from a landrace. The cultivation time of sweet cherry (Prunus avium L.) is much shorter than that of Chinese cherry in Zhejiang province. Growers and researchers try their best to plant sweet cherry because of their high average value even if the climate is not good enough for sweet cherry, such as a winter not being cold enough and summer being too hot and too wet. The researches were concentrated on cultivar selection which was adapted to Zhejiang’s climate, and technology set points to optimize growing condition effectively. The new marked improvement in Zhejiang sweet cherry production was growing economic yields in the last 3 years. In order to get economic yields, the new research improvement was obtained: 1) at least four cultivars were proven suitable for Zhejiang from the test on introduction and domestication in the combination of orchard management; 2) the combination of plant technology for sweet cherry was got a patent right in China; 3) the new selection ‘Jiangnanhong’, which comes from ‘Hongdeng’ × ‘Lapins’, got cultivar identification in Zhejiang Province. Anticipation of the cherry future in the warm winter and hot summer regions is also introduced in the article.

We measured missing mass spectrum of the ^{12}C(γ,p) reaction for the first time in coincidence with potential decay products from η^{'} bound nuclei. We tagged an (η+p) pair associated with the η^{'}N→ηN process in a nucleus. After applying kinematical selections to reduce backgrounds, no signal events were observed in the bound-state region. An upper limit of the signal cross section in the opening angle cosθ_{lab}^{ηp}

Herein, a simple green co-precipitation route for Mn

To evaluate the coverage of different levels of axillary lymph nodes and organs at risk according to the field design of AMAROS study (levels I-II-III-IV), breast tangents with supraclavicular and infraclavicular fields (levels II-III-IV) and high tangent fields to the breast after breast-conserving surgery.We delineated the axillary lymph nodes levels I-IV in 34 patients treated with breast-conserving surgery and sentinel lymph nodes biopsy. Field design according to AMAROS study - levels I-IV in patients without axillary dissection - as well as irradiation of levels II-IV used in N+ patients after axillary dissection, and also high tangent fields was simulated. Mean dose levels and volumes covered by 95% or 80% isodoses were evaluated. Doses to ipsilateral lung, heart and brachial plexus were compared. Paired t test was used.AMAROS study and levels II-IV plans delivered therapeutic dose to high axilla (levels II-IV), but the high tangent fields showed inefficacy to cover these volumes, P0.001). In terms of organs at risk, especially, ipsilateral lung, AMAROS study plan was found to significantly increase the volume receiving at least 10Gy (I-IV:46.8%, II-IV: 39%), but also the volume receiving at least 20Gy (I-IV: 39.3%, II-IV: 31.3%), and VThe omission of axillary dissection and the axilla irradiation need is associated with high dose irradiation of the lungs, and with higher toxicity. The indication of axillary dissection or irradiation of low axilla could be individualized in relation with individual comorbidities and factors of risk.

Magnetic resonance angiography (MRA) is a non-invasive vascular imaging technique. It can resolve the 3D morphology and structure of the circle of Willis when used in conjunction with three dimensional time-of-flight (3D TOF) and maximum intensity projection (MIP) technology. However, slow blood flow or saturation effects will cause signal loss in 3D TOF, and affect the accuracy of diagnosis. The balanced fast field echo (BFFE) technique employs a fully-balanced gradient waveform along all three axes, which allows magnetization to reach a steady state, providing fluids with good signal-to-noise ratios (SNRs) and excellent tissue contrast. Balanced fast field echo can then be used as diagnostic pulse sequence for blood vessels or fluid-filled tissues. Two case reports illustrate the value of BFFE-assisted 3D TOF to diagnose intracranial arterial stenosis.

AIM Diabetes is a risk factor in infective endocarditis. However, few studies have focused on the prognostic value of prediabetes in infective endocarditis. This analysis aimed to explore the relationship between prediabetes and outcomes for people with infective endocarditis. METHODS Diabetes and prediabetes definitions were based on the American Diabetes Association 2014 criteria. A total of 866 people who had been consecutively diagnosed with infective endocarditis between January 2009 and July 2015 were included in the analysis. They were divided into three groups: normoglycaemia (n = 469), prediabetes (n = 246) and diabetes (n = 151). Univariate and multivariate analyses were used to identify risk factors for adverse outcomes. RESULTS Overall in-hospital mortality was 8.5% (74 of 866), and differed significantly among the normoglycaemia, prediabetes and diabetes groups (3.4%, 12.6% and 17.9%, respectively; P < 0.001). Compared with the normoglycaemia group, the adjusted odds ratio for in-hospital death was 2.42 [95% confidence interval (CI) 1.11-5.31; P = 0.027) for prediabetes and 3.39 (95% CI 1.48-7.80; P = 0.004) for diabetes. The cumulative long-term death rate was significantly higher in the prediabetes or diabetes groups than in the normoglycaemia group (log-rank = 34.82; P < 0.001). CONCLUSION In addition to diabetes, prediabetes was also associated with a higher risk of in-hospital and long-term mortality among people with infective endocarditis. Therefore, attention should be paid to this population.

In chemical kinetic model reduction under internal combustion engine conditions, most implementations only consider ignition related chemistry without consideration of flame speed prediction. In practice, flame propagation commonly exists in spark ignition engines, dual-fuel with pilot injection compression ignition engines, reactivity controlled compression ignition engines, and etc. Due to the inherent time-consuming nature, it is impractical to run a 1-D flame code with trial-and-error methods for model reduction, especially when starting with a large chemical kinetic model. In this paper, an improved reduction methodology is proposed for construction of a small set of species that give accurate predictions of both flame speeds and ignition delays. First, a strong correlation is found between the errors of maximum H radical and the errors in prediction of laminar flame speeds. Addition of H to the search targets in graph-based methods is conducted showing improvement in accuracy of flame speed prediction. Second, the normalized flame speed sensitivity with rate constants is analyzed for identifying a set of species that strongly influences the prediction of flame speeds. Finally, a trial-and-error based method is used for further reduction with a 0-D testbed for prediction of ignition only, while keeping the species important to flame chemistry. The newly proposed reduction methodology is used for development of accurate skeletal models predicting both ignition and flame speeds for several hydrocarbon fuels. These skeletal models include methane (27 species), propane (32 species), n-heptane (126 species), and primary reference fuel gasoline surrogates (207 species) with high fidelity to be used in engine simulations.

Aims: Previous evidence has demonstrated that participation in the Dose Adjustment for Normal Eating (DAFNE) education programme can reduce HbA1c and severe hypoglycaemia in people with Type 1 diabetes. In a number of studies, increased HbA1c variability has been associated with higher diabetic morbidity and mortality. No studies have examined the impact of structured education on HbA1c variability in Type 1 diabetes. Methods: People with Type 1 diabetes who had attended DAFNE were identified for inclusion from the Scottish Care Information‐Diabetes dataset. HbA1c median and variability, expressed as coefficient of variation (CV) before and after DAFNE was calculated. Results: Some 1061 individuals participated in DAFNE education and 687 met the inclusion criteria. A significant median reduction in HbA1c [−3.5 mmol/mol (−0.3%)] was seen at 12 months with a significant reduction [−1.5 mmol/mol (−0.1%)] still seen at 60 months of follow‐up. HbA1c variability as measured by CV was significantly lower during the post‐DAFNE period: 0.08 (IQR 0.05–0.12) reduced to 0.07 (IQR 0.05–0.10); P = 0.002. Conclusion: The data confirm that DAFNE participation improves glycaemic control in Type 1 diabetes with benefits being sustained for 5 years. This study is the first to demonstrate reduced HbA1c variability after completion of structured education. This is new evidence of the beneficial impact of DAFNE on glycaemic profile.

Micro-electrochemical machining (micro-ECM) has been widely used for microscale and nanoscale processing of materials. The performance of the nanosecond pulse power supply is directly related to the precision of micro-ECM, which is one of the core technologies for micro-ECM. In this work, a nanosecond pulse power supply, with adjustable pulse frequency, duty cycle, and voltage, was designed with an STM32F103VET6 single-chip microcomputer as the control core and a metal-oxide-semiconductor field-effect transistor as the chopper switch component. The performance test has shown that the power supply can produce a continuous pulse with the highest frequency of 8 MHz, the shortest pulse width of 50 ns, the maximum peak current of 12 A, and the maximum voltage of 10 V. As compared with the power supply reported in the literature, the present power supply demonstrated the enhanced output current and improved waveform of the nanosecond pulse output, which could result in better machining accuracy and efficiency for micro-ECM.

Fermented soybean meal (FSM), which has lower anti-nutritional factors and higher active enzyme, probiotic and oligosaccharide contents than its unfermented form, has been reported to improve the feeding value of soybean meal, and hence, the growth performance of piglets. However, whether FSM can affect the bacterial and metabolites in the large intestine of piglets remains unknown. This study supplemented wet-FSM (WFSM) or dry-FSM (DFSM) (5% dry matter basis) in the diet of piglets and investigated its effects on carbon and nitrogen metabolism in the piglets' large intestines. A total of 75 41-day-old Duroc×Landrace×Yorkshire piglets with an initial BW of 13.14±0.22 kg were used in a 4-week feeding trial. Our results showed that the average daily gain of piglets in the WFSM and DFSM groups increased by 27.08% and 14.58% and that the feed conversion ratio improved by 18.18% and 7.27%, respectively, compared with the control group. Data from the prediction gene function of Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) based on 16S ribosomal RNA (rRNA) sequencing showed that carbohydrate metabolism function families in the WFSM and DFSM groups increased by 3.46% and 2.68% and that the amino acid metabolism function families decreased by 1.74% and 0.82%, respectively, compared with the control group. These results were consistent with those of other metabolism studies, which showed that dietary supplementation with WFSM and DFSM increased the level of carbohydrate-related metabolites (e.g. 4-aminobutanoate, 5-aminopentanoate, lactic acid, mannitol, threitol and β-alanine) and decreased the levels of those related to protein catabolism (e.g. 1,3-diaminopropane, creatine, glycine and inosine). In conclusion, supplementation with the two forms of FSM improved growth performance, increased metabolites of carbohydrate and reduced metabolites of protein in the large intestine of piglets, and WFSM exhibited a stronger effect than DFSM.

Simultaneous point measurements of temperature, mixture fraction, major species (H 2 , H 2 O, O 2 , N 2 ) concentrations from KrF laser-induced spontaneous Raman scattering and minor species (OH) concentrations from KrF laser-induced predissociative fluorescence (LIPF) in unswirled ( S g = 0), low swirl ( S g = 0.12), and high swirl ( S g = 0.5) lifted turbulent hydrogen jet diffusion flames into still air are reprocessed to obtain profiles of the Favre-averaged scalars and conditional moments. Large discrepancies between the Favre-averaged and ensemble-averaged temperature, H 2 O, and OH mole fractions are found at the lifted flame region, due to density weighting of fairly large samples of unreacted mixtures. Conditional statistics are used to reveal the reaction zone structure in mixture fraction coordinates. The cross-stream dependence of conditional means of temperature and species concentration is found to be significant in the lifted flame region of the swirled flames and decreases to negligible levels with increasing streamwise position. Comparison of the measured conditional mean variation of OH vs. H 2 O with a series of stretched laminar partially premixed flame and diffusion flame calculations reveals that for the unswirled flame, the differential molecular diffusion and radial dependence of conditional means are minor at x/D = 6.4 for stretch rates from a = 14,000 to 400 s -1 . For the low and high swirling flames, however, the measured OH vs. H 2 O conditional means at the lifted flame region are not consistent with stretched laminar flame calculations. The level of partial premixing and the stretch rate decrease with increasing downstream locations. The estimated stretch rate at x/D = 53.5 is about 50–10 s -1 , while at x/D = 107 the stretch rate is about 10 s -1 with some measurements at adiabatic equilibrium.

Aim To compare the tolerability and effectiveness of ferric carboxymaltose (FCM) and iron sucrose (FeS) in pregnant women with iron deficiency (ID). Methods Retrospective audit of pregnant women in their second or third trimesters receiving intravenous (IV) FCM or FeS at an Australian tertiary maternity hospital from January 2007 to July 2013. Demographics, haematological and infusion details, including adverse events and pregnancy outcomes, were compared using Student's t-test, Chi-square and Fisher's exact tests. Results Eighty-three women had 94 infusions (56 FCM requiring 2 h in Day Stay; 38 FeS requiring 6 h in Acute Care) administered. There were no significant demographic differences between groups. Neither group had anaphylactic reaction or unplanned admission after infusion. Non-anaphylactic adverse reactions at first infusion were lower in the FCM group (16 vs 34%, p = 0.049). Pre-infusion mean Hb and ferritin were 98.1 g/L ± 10.8 g/L and 9.7 μg/L ± 6.2 μg/L in the FCM group and 98.3 g/L ± 11.7 g/L and 8.0 μg/L ± 3.9 μg/L in the FeS group. Post-infusion, both groups had significantly higher Hb and ferritin: rises of 12.7 g/L and 296 μg/L, respectively for FCM (p < 0.001 vs all pre-infusion values) and rises of 8.9 g/L and 105 μg/L in FeS (p < 0.05 vs all pre-infusion values). Pregnancy outcomes, including post partum haemorrhage rates, were similar between groups. Conclusion Although haematological parameters improved following infusion in both groups, FCM had a lower incidence of infusion-related adverse events compared to FeS. Although sample size is small, given decreased monitoring and infusion time required for FCM, it may become the preferred formulation for ID treatment in pregnancy.

Background: Contrast media is widely used in clinical diagnostic and interventional procedures, but may cause damage to the kidney, that is, contrast-induced nephropathy. This study was to establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of renal function markers cystatin-C (Cys-C) and β2-microglobulin (β2-MG) for the early diagnosis and follow-up surveillance of contrast-induced nephropathy.Methods: A sandwich immunoassay was used to detect the concentration of Cys-C, and the competitive immunoassay was used to detect the concentration of β2-MG in 50 samples of urine. The performance of this dual-label TRFIA was evaluated and compared with commercial assays.Results: The sensitivity for Cys-C detection was 1.26 ng/ml, the average recovery was 99.36%; The sensitivity for β2-MG detection was 2.13 ng/ml, the average recovery was 100.18%. Bland–Altman analysis showed that the dual-label TRFIA method and the commercial kits had a good agreement, s...

We measured missing mass spectrum of the ^{12}C(γ,p) reaction for the first time in coincidence with potential decay products from η^{'} bound nuclei. We tagged an (η+p) pair associated with the η^{'}N→ηN process in a nucleus. After applying kinematical selections to reduce backgrounds, no signal events were observed in the bound-state region. An upper limit of the signal cross section in the opening angle cosθ_{lab}^{ηp}-0.9 was obtained to be 2.2 nb/sr at the 90% confidence level. It is compared with theoretical cross sections, whose normalization ambiguity is suppressed by measuring a quasifree η^{'} production rate. Our results indicate a small branching fraction of the η^{'}N→ηN process and/or a shallow η^{'}-nucleus potential.

MicroRNAs (miRNAs) are endogenous, non-coding RNAs, which exert crucial functions in regulating biological progressions. Previous studies have demonstrated the anti-tumor effect of miRNA-215-5p. However, its specific role in influencing the progression of prostate cancer (PCa) remains unclear. This study aims to uncover the regulatory effect of miRNA-215-5p on the metastasis and prognosis of PCa.MiRNA-215-5p levels in collected PCa tissues (n=52) and paracancerous tissues (n=52) were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The relationship between miRNA-215-5p level and pathological indexes, as well as overall survival of PCa patients, was analyzed. Regulatory effects of miRNA-215-5p on proliferative and metastatic capacities of LNCaP and DU-145 cells were evaluated through cell counting kit-8 (CCK-8) and transwell assay, respectively. Bioinformatics prediction was performed to search for the target genes of miRNA-215-5p and PGK1 was selected. The biological role of PGK1 in the progression of PCa was finally clarified by a series of rescue experiments.MiRNA-215-5p was lowly expressed in PCa tissues and cell lines. Low level of miRNA-215-5p predicted poor prognosis in PCa patients. The silence of miRNA-215-5p enhanced viability, migratory, and invasive capacities of LNCaP cells, while the overexpression of miRNA-215-5p yielded the opposite trends in DU-145 cells. PGK1 was predicted to be the target of miRNA-215-5p. PGK1 was upregulated in PCa tissues and cell lines and its high level predicted poor prognosis of PCa. Moreover, PGK1 level was negatively correlated to that of miRNA-215-5p in PCa tissues. PGK1 was able to reverse the regulatory effects of miRNA-215-5p on metastatic potentials of PCa cells.Downregulated miRNA-215-5p in PCa is closely related to distant metastasis and poor prognosis of affected patients. MiRNA-215-5p alleviates the malignant progression of PCa by targeting and downregulating PGK1.

Primary angle-closure glaucoma (PACG) is the most common type of glaucoma in China. In recent years, lens extraction has gradually become one of the main means of PACG treatment. However, there are still some problems in specific applications. In this article, we analyze the problems and misunderstandings in lens extraction for treatment of PACG in China at present, hoping to achieve reasonable application and better serve patients with glaucoma.原发性闭角型青光眼（PACG）是我国最常见的青光眼类型。近年来晶状体摘除手术已逐渐成为PACG的治疗方法之一。本文针对现阶段我国晶状体摘除手术在PACG治疗中存在的问题及误区，提出应根据房角关闭的不同机制合理选择治疗方法，正确看待透明晶状体摘除术在PACG治疗中的作用，注意我国与西方国家医疗体系的差异以及联合房角分离术或滤过性手术的效果，以期指导临床合理应用晶状体摘除手术治疗PACG，达到为青光眼患者提供优质服务的目的。（中华眼科杂志，

Acroporidae and Pocilloporidae are dominant genera in Indo-Pacific coral reefs, forming over half of the reef entities. However, how corals build their reef colonies is still obscure. Here, we reconstruct the skeletal and internal structures of twelve representative reef colonies with high-resolution computed tomography to investigate the relationship between coral growth and reef-forming patterns, coral growth axis and basic coral growth parameters. We quantified the skeletal ratio of reef colonies, the amount of mineralisation at different coral growth stages and the area of effective light absorption via colony reconstructions. Our work revealed that the reef skeleton structures are highly related to their growth rate and tolerance to environmental stress. The colonies with high growth rate and susceptibility to external disturbances had abundant canals among adjacent calices, while colonies with stress tolerance had thick surface skeletons and sealed calices. We visualized the growth axes of these coral species, which can reveal the growth trend of coral colonies and divide these main coral species into three categories. Our results deepen our knowledge about the ecological system of various coral reefs and will help in coral reef restoration by guiding the selection of suitable coral species.

Recently, the high growing semiconductor is the Internet of Things (IoT) and fifth generation (5G) connectivity application. Based on 5G, the different working frequency between sub 6GHz and millimeter wave (mmWave) frequency range on 28GHz and 39GHz, both systems provide good networking coverage in the city. The mmWave antenna is designed by array antenna to beamforming the Radio frequency signal to the networking devices to achieve high speed data transmission requirement. The system in package (SiP) could integrate various heterogeneous components such as antenna in package (AiP) to get small form factor and high electrical performance. The electromagnetic interference (EMI) is very critical which can damage electronic devices and equipment. To meet the high frequency of electrical performancents, the partition EMI shielding has provided the signal isolation between different function blocks such as digital, analog and RF antenna devices in SiP module. In this paper, a test vehicle is designed to evaluate the shielding effectiveness (SE) by vector network analyzer (VNA). The different partition EMI shielding method and study will proceed and measure under mmWave frequency of 22GHz to 40GHz. The reliability verification of EMI shielding on advanced SiP module will utilize standard JEDEC test condition including Temperature Cycle Test (TCT), High Temperature Storage (HTS), unbias-high accelerated stress test (u-HAST) results to verify the package structure. Finally, this paper will demonstrate the advanced SiP with EMI shielding solutions for future 5G Connectivity devices application.

Checkpoint inhibition has emerged as a promising therapeutic strategy for several types of cancer. Immune-related adverse effects (irAEs) commonly involve the endocrine system. Distinguishing endocrine side effect-related symptoms from disease progression or treatment-related toxicity can be challenging. If not recognized in time, endocrine irAEs may be life-threatening. As the use of checkpoint inhibitors is expected to increase, there is a need for more awareness of endocrine irAEs amongst health care professionals. We describe three cases that illustrate the importance of timely recognition, patient education and the management of endocrinopathies. Two patients who were treated with the checkpoint inhibitor ipilimumab developed hypophysitis and subsequent episodes of hypocortisolism. These cases underline both the frequency of diagnostic delay and the importance of patient education with regard to glucocorticoid stress dosage. The third patient presented with diabetes mellitus after administration of nivolumab. A multidisciplinary approach is warranted to ensure optimal care for patients with endocrine irAEs.

The 5G revolution brings a lot innovative impact in various applications and business opportunities in mobility, automotive, insdustrial automation and healthcare globally. Therefore, the muti-function integration, form factor miniaturization and time to market of IC package design become the necessary trend in electronic assembly technology. Obviously, system in package (SiP) is the capable solution to meet these requirments and widely applied in the IOT, automotive and sensor module currently. However, the conventional singulation process by blade sawing technology exists the burr residual, inflexible package shape forming and inaccuracy tolerance controlling challenges that brings about low yield and limited package shape design.In this research, the sigulation and metal lid by laser ablation technology in various system in package applications are demonstrated, the metal lid discoloration and burr residual defeat are successfully eliminated by pico second pulses laser technology which shorten the energy absorption time from laser light to electrons and improve the heat-affected zone (HAZ) damage on sawing edge during the ablation process. Therefore, the design of experiment (DOE) shows that the metal lid discoloration rate have been greatly improved from 25% to 0% and without the burr residual issue by utilizing UV laser ablation process. In addition, the package shape forming by UV laser is also studied in this paper, the various geometrical shape that including circle, hexagon and qctagon package appearance are fabricated within 30 um cutting tolerance while without any chipping residual on cutting edge, thus, this formnig technology have been widely applied in various fingerprint sensor and particular module applications in smartphone and automotive industry.The typical reliability testing (Temperature Cycle Test, Hight Temperature Storage Test and unbias HAST) results as a verification. By employing this advanced UV laser ablation technology, the package forming quality and sawing accuracy control can be improved obviously compare to the topically blade sawing process. Undoubtedly, the UV laser ablation with pico pluse technology is absolutely the innovative and powerful weapon to accomplish the high quality SiP design in 5G generation.

Recently, the coming fast and large growing opportunity market will be the Internet of Things (IoT) and fifth generation (5G) connectivity application. Particularly for 5G, need to design by beamforming antennas to send Radio Frequency (RF) signals to different network & client devices. By using MIMO (Multi-Input Multi-Out) technology which uses more transmitters and receivers to ensure each antenna of data transmission performance. To meet those new application requirements & challenges, high-gain antennas have been proposedfor wireless connectivity products such as high data transmission, very low latency and low power consumption requirements. A 3D System in Package (3DSiP) including different approach, such as the double side assembly technology and antenna in package (AiP) which is a combination solutions for these requirements. In this paper, SPIL provided an alternative 3D antenna SiP technologu which used surface mount technology (SMT) and 3D antenna integration were designed to shrink the package size, the calculation of package size can be shrunk around 55% area andpackage size reducedfrom 10 x8mm to 6x6mm. This new solution could be integrated an antenna inside SiP module as AiP technology to get smallform factor and additional major benefites to address cost, performance, and time-to-market. The characterization analysis will utilize typical reliability testing (Temperature Cycle Test, High Temperature Storage Test, unbias HAST) results and verify the package structure. Finally, this paper will find out the suitable 3DSiP with advanced antenna integration solution forfuture IoT and 5G Connectivity devices application.

To explore the influences of high glucose on the proliferation and apoptosis of prostate cancer cells and analyze its possible mechanism of action.Human prostate cancer cell line LNCaP was divided into control group, mannitol group, and high glucose group. Then, the proliferation in each group was detected via methyl-thiazolyl-tetrazolium (MTT) assay. Hoechst staining assay was performed to determine the apoptosis level in each group. Western blotting was employed to measure the expression levels of apoptosis-related proteins and nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and γ-glutamylcysteine synthetase (γ-GCS) proteins. The cellular reactive oxygen species (ROS) level was measured through 2,7-dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay. Enzyme-linked immunosorbent assay (ELISA) was carried out to detect the content of lactate dehydrogenase (LDH) and inflammatory factors.High glucose significantly promoted the proliferation of prostate cancer cells LNCaP (p0.01) and increased the apoptosis level of cells (p0.01). In high glucose group, the expression level of Caspase-3 protein was overtly increased (p0.01), while that of B-cell lymphoma-2 (Bcl-2)/Bcl-2 associated X protein (Bax) was significantly decreased (p0.01). High glucose group had clearly increased the content of ROS (p0.01), LDH (p0.01), and interleukin-6 (IL-6) (p0.01), but decreased the content of IL-10 (p0.01). High glucose notably lowered the protein expression levels of Nrf2, HO-1, and γ-GCS in the cells (p0.01).High glucose represses the activation of the Nrf2/anti-oxidation response element (ARE) signaling pathway in prostate cancer cells and increases the content of ROS, IL-6, and the expression of apoptotic proteins in the cells, thus promoting the apoptosis of prostate cancer cells.

Author(s): Aidala, CA; Arrington, JR; Ayuso, C; Bowen, BM; Bowen, ML; Bowling, KL; Brown, AW; Brown, CN; Byrd, R; Carlisle, RE; Chang, T; Chang, WC; Chen, A; Chen, JY; Christian, DC; Chu, X; Dannowitz, BP; Daugherity, M; Diefenthaler, M; Dove, J; Durandet, C; El Fassi, L; Erdos, E; Fox, DM; Geesaman, DF; Gilman, R; Goto, Y; Guo, L; Guo, R; Hague, T; Hicks, CR; Holt, RJ; Isenhower, D; Jiang, X; Katich, JM; Kerns, BM; Kinney, ER; Kitts, ND; Klein, A; Kleinjan, D; Kras, J; Kudo, Y; Lin, PJ; Liu, D; Liu, K; Liu, MX; Lorenzon, W; Makins, NCR; Martinez, JD; McClellan, RE; McDonald, B; McGaughey, PL; McNease, SE; Medeiros, MM; Miller, B; Miller, AJ; Miyasaka, S; Miyachi, Y; Mooney, IA; Morton, DH; Nadim, B; Nagai, K; Nakahara, K; Nakano, K; Nara, S; Obata, S; Peng, JC; Prasad, S; Puckett, AJR; Ramson, BJ; Raymond, RS; Reimer, PE; Rubin, JG; Salinas, R; Sanftl, F; Sawada, S; Sawada, T; Scott, MBC; Selensky, LE; Shibata, TA; Shiu, S; Su, D; Tadepalli, AS; Teo, M; Tice, BG | Abstract: The SeaQuest spectrometer at Fermilab was designed to detect oppositely-charged pairs of muons (dimuons) produced by interactions between a 120 GeV proton beam and liquid hydrogen, liquid deuterium and solid nuclear targets. The primary physics program uses the Drell–Yan process to probe antiquark distributions in the target nucleon. The spectrometer consists of a target system, two dipole magnets and four detector stations. The upstream magnet is a closed-aperture solid iron magnet which also serves as the beam dump, while the second magnet is an open aperture magnet. Each of the detector stations consists of scintillator hodoscopes and a high-resolution tracking device. The FPGA-based trigger compares the hodoscope signals to a set of pre-programmed roads to determine if the event contains oppositely-signed, high-mass muon pairs.