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Urinary metabolites and biological half-life of chlorpyrifos (O, O-diethyl-O-3,5,6-trichloro-2-pyridinyl phosphorothioate) were investigated. Male Wistar rats weighing 200 g were intraperitoneally injected with chlorpyrifos at a level of 0.2 mmol/kg body weight. Both chlorpyrifos and 3,5,6-trichloro-2-pyridinol (TCP) levels in blood showed maximum values at 5 h post-injection, and then decreased rapidly. Biological half-lives of the blood chlorpyrifos and TCP were estimated to 8.15 and 24.66 h, respectively. Urine was collected for 96 h post-injection and hydrolyzed with 4 N HCl or beta-glucuronidase with sulfatase, and TCP released was determined. Urinary excretion levels of the acid hydrolysis-released TCP and the enzyme hydrolysis-released TCP accounted for 86 and 54% of chlorpyrifos administered, respectively. Urinary excretion levels of alkylphosphate for 96 h post-injection were analyzed. The excretion levels of diethylthiophosphate (DETP) and diethylphosphate (DEP) accounted for 45 and 15% of chlorpyrifos administered, respectively. These results indicate that 1) about half of the chlorpyrifos administered was directly hydrolyzed to DETP and TCP, 2) 10 to 20% was hydrolyzed to DEP and TCP after the oxidation to chlorpyrifos oxon, and 3) about 30% was dealkylated to TCP-phosphate after the oxidation.

糊熟期および完熟期のトウモロコシを用いてバッグサイロによってサイレージを調製し,添加剤の使用が開封後の好気的変敗を防止する効果を調べた.添加剤としては,カプロン酸(10mmol/kg),カプロン酸+塩酸(6N-HCl,20ml/kg),および市販の添加剤(ギ酸カルシウム75%,安息香酸ナトリウム10%,重亜硫酸ナトリウム10%およびミネラル5%を含む.'MK'剤と略す)0.25%を用い,詰込み時に添加する区と開封時に添加する区とを設けた.開封後のサイレージを発泡スチロール製の円筒形断熱容器(径30cm,深さ27cm,厚さ1.5cm)に詰め,表面を空気に曝して,約25°Cの室内に7日間放置し,この間のサイレージの温度の経時的変化を調べ,また開封時と7日後のサイレージのpH値と有機酸組成,あるいは酵母とカビの菌数を測定して,変敗の有無を判定した.その結果,材料の異なる2回の実験とも同様に,無添加の対照サイレージでは,温度とpHの上昇が起こり,酵母数が増加して明らかに変敗したのに対し,開封時にカプロン酸あるいはカプロン酸+塩酸を添加した区ならびに詰込み時にカプロン酸を添加した区においては,開封後7日間にわたって温度の上昇が認められず,好気的変敗は防止された.一方,詰込時にカプロン酸+塩酸を添加した区では,pHの上昇は認められず,2.8～3.2であったにもかかわらず,温度は上昇し酵母数も増加して,変敗を完全に防止することはできなかった.'MK'剤については,詰込み時添加では効果が十分に認められず,開封時添加によって7日間にわたり変敗を防止することができた.

Quantitative and qualitative examination of Polychlorinated Biphenyl (PCB) residues in blood of the women occupationally exposed to PCBs and of their children has been carried out. The children examined in this study have ingested their mothers' milk for periods of 0 to 3 years. The age of the children at the time of this study ranges from 0 to 13 years. The data obtained in this examination provide evidence that PCBs are retained in the children's body for many years and a longer feeding of mothers' milk tends to increase PCB levels in the blood of the children. Especially high PCB levels, 93.2 ppb (μg/kg) and 77.4 ppb are found in the blood of two children. It is found that the gas chromatographic patterns of blood PCBs of the children who have been fed on their mothers' milk for a long time, are different from those of their mothers.

Egg yolk lipoproteins, low density lipoprotein fraction and high density lipoprotein fraction, were incubated with metallic salts for the periods of ten days. Fluorescence intensity at Em 430455nm, Ex 355390nm in the incubated solution increased by oxidation of the lipid moiety of lipoproteins catalyzed by metalic ions. Changes of lipoproteins were prevented by addition of ethylenediaminetetraacetic acid (EDTA).On the other hand, the ultrasonic irradiation on egg yolk lipoproteins provokes oxidation of the lipid moiety of lipoproteins, but no development of fluorescence spectrum was observed in the course of oxidation by the ultrasonic irradiation. These results suggest that oxidation occurs in the lipid moiety of lipoproteins during the ultrasonic irradiation similar to the case of the lipoprotein oxidized by metalic ion catalysis, but the increase of fluorescence intensity is not accelerated by the ultrasonic irradiation.

This paper concerns the flow of highly rarefied gas through a circular tube of finite length leading to a vacuum from a reservoir. First, the free molecular flow is considered. Then, the effect of intermolecular collisions is estimated by applying the iteration method to the B-G-K equation. The total mass flux through the tube is computed for several values of tube length. It is shown that the correction of the flux to the free molecular flow decreases with increasing tube length and becomes negative for a tube whose length is more than about eight times as large as its diameter.

A determination method for choline-containing phospholipids in serum lipoproteins was established by application of the HPLC method [Hara et al. (1980) J. Biochem. 87, 1863-1865; (1981) 89, 879-887] developed for cholesterol quantitation. The concentration of choline-containing phospholipids in the fraction separated by HPLC using gel permeation columns was determined through colorimetric detection using a commercial enzymatic kit. The optimum conditions for enzymatic reaction in the flow diagram were examined. The concentration calculated from the A500 peak area was found to reflect very precisely the concentration of choline-containing phospholipids in all lipoprotein fractions, and the quantitation of each lipoprotein fraction can be performed with only 10-20 microliters of whole serum. Monitoring the elution patterns by detecting choline-containing phospholipids can give much more information about lipoprotein distributions according to particle size than analyses done by detecting cholesterol.

A series of alkylphosphorylcholines bearing alkyl group of C3C22 were synthesized to investigate the physico-chemical properties as the model of phosphatidyl choline.Alkylphosphorylcholines were white crystals and had the melting points of over 200°C. We could not find any IR absorption at 1740cm-1 (CO str.) and 1180cm-1 (C-O-C str.). They dissolved easily in water and especially C18, C20, and C22 -homologues showed clearly Krafft points at 21.5°C, 39.0°C and 53.5°C, respectively. Alkylphosphorylcholines of C10C16 formed also micelle structure.They dissolved easily in methanol and chloroform. The solubility in chloroform was attributed to the formation of hydrogen bondings between phosphoryl choline group and chloroform molecule.No solubility was observed in benzene and carbon tetrachloride for all alkylphosphorylcholines.

Chlorpyrifos (O, O-diethyl-3,5,6-trichloro-2-pyridinyl phosphorothioate; CP) has been used for insecticide to prevent infestation of termites. A study was made on occupational exposure to CP of termite control workers engaged in spraying this organophosphorus insecticide. Workers were engaged in two types of spraying; that is spraying CP under floor and spraying CP around house. All the under-floor sprayers wore respirators during spraying. CP levels in air near the breathing zone, contact amounts of CP with skin, and urinary alkylphosphates levels were higher in the under-floor sprayers than in the around-house sprayers. The personal ambient CP levels of the under-floor sprayers were 0.012 to 0.145 (8 h-TWA, mg/m3) lower than the threshold limit value (0.2 mg/m3) proposed by ACGIH. A maximum diethylphosphate (DEP) excretion was observed in the first urine collected the next day, while the highest diethylthiophosphate (DETP) was excreted in the last urine in the working day. In the excretion amount, DEP was higher than DETP. Amounts of CP collected in respirators used by the under-floor spraymen averaged 0.76 mg/respiratory comparable to their estimated theoretical value of 0.69 mg/m3. The urinary total alkylphosphate (DEP + DETP) level was more closely correlated with the contact amount of CP with skin (r = 0.86) than with the personal ambient CP level (r = 0.05). These results indicate that 1) respiratory absorption of CP was prevented by the use of respirator; 2) the urinary alkylphosphates were mainly derived from the dermal absorption of CP; and 3) biological monitoring using urinary alkylphosphates levels is an useful index for CP exposure.

Surface pressures and surface viscosities of dipalmitoylphosphatidylethanolamine and its polar group analogues were measured at the hexane/water interface as a function of bulk pH. The monolayers expanded as the bulk pH was shifted from neutral to alkaline and gave rise to an increase in surface pressure at a constant area, and the surface viscosity was simultaneously reduced at high pH. For the increase in surface pressure, theoretical values were calculated using the Gouy-Chapman equation for the electrical double layers produced by shifting pH, and good agreements were obtained with the measured ones from which a simple mechanism was deduced for the increase in pressure. The ionic dissociation characteristics of amino groups of the lipids were discussed taking p K a values given in the above calculations into account. The reduction of the surface viscosity was thought to be attributable to disintegration of zwitter ionic structure in the condensed monolayers.

The flight surveys were conducted in the waters off the southeast coast of Hokkaido where a big catch of sardine Sardinops melanosticta has been taken during summer and autumn seasons. In order to examine the swimming speeds of schools, two kinds of survey, such as experiments and observations, were conducted near the fishing ground on Oct. 9 and Sept. 29, 1985 respectively. In the experiments five floaters were airdropped at a regular interval to intersect the target schools from the rear to the front. After that, relative changes between the school and the floaters were compared. On the other hand, the observations was carried out shuttling the same transect in order to observe the movements of a number of schools. From the results of the experiments the cruising speeds lie between 1.2-4.1BL/s (0.4-1.5kt). The result obtained by the observation was 4.3kt, which was larger compared with the data described above. The schools which were chosen as the subject in the experiments were elongate in shape and the longest dimensions of the schools ranged from 300-1, 500m. The results may suggest that the size of schools have an effect on the speed, as the speed of school decreases with an increase in size.

Long-term studies on the concentrations of polychlorinated biphenyls (PCBs) in the blood of children and their mothers occupationally exposed to PCBs were made during the period 1975 to 1979. This report deals in particular with the rates of decrease and half-lives of PCB concentrations in the blood. Rates of decrease of the concentration of PCBs in the blood of both mothers and their children were relatively constant and independent of PCB concentrations. Therefore, a one-compartment model equation was sufficient to represent the decrease of the concentrations of PCB in the blood. The mean rate constant of the decrease of PCB concentration for the children was approximately 2.6 times higher than that of the mothers.

A new technique for serum lipoprotein quantitation by high performance liquid chromatography (HPLC) was evaluated by comparing the analytical data with the electrophoretic method using agarose gel film. The elution pattern of human serum (5μl) monitored by cholesterol gave three clear peaks according to particle-size differentiation: peak 1, chylomicrons+very low density lipoproteins (VLDL); peak 2, low density lipoproteins (LDL); peak 3, high density lipoproteins (HDL). As for a group (n=92) of normolipidemic men and women, cholesterol levels in these three fractions were determined by the HPLC method (X), and they were compared with those by the electrophoretic method (Y). A good correlation was obtained for peak 2 to β lipoprotein (r=0.961, Y=0.990X+3.43) and for peak 3 to α lipoprotein (r=0.911, Y=0.945X+7.21). Cholesterol level of peak 1 (chylomicrons +VLDL) was 5.3±4.0mg/dl, but that of pre-β liporrotein could not be measured by the electrophoretic method. Precision of determination (coefficient of variation, CV%) by the HPLC method was less than 1% both for peak 2 (LDL) and peak 3 (HDL). The HPLC method was found to have better precision and resolution for lipoprotein quantitation than the electrophoretic method.

Organic solvent products used in the various factories were collected nationwide in Japan, and analyzed by gas chromatogarphy. Of the 1179 samples collected, 522 samples were homogeneous (including 321 thinners, 145 degreasers and 56 reagents and others), and were analyzed by means of direct injection into a FID-gas chromatograph with two FSWCOT (OV 101 and PEG 6000) capillary columns. In thinners (n=248), the average number of solvent components per sample was 4.14 when the thinners containing gasoline were excluded. Toluene was the most popular component in thinners (present in 56%) followed by ethyl acetate, methanol, xylenes, ethylbenzene, isobutyl alcohol, ethyleneglycol mono-ethylether acetate, butyl acetate and methyl isobutyl ketone. Gasoline was also detected in 23%. In degreasers (n=123), the average number of solvent components per sample was 1.64 when the degreasers containing gasoline were not taken into account. Trichloroethylene was present in 21% of degreasers followed by tetrachloroethylene, 1, 1, 1-trichloroethane, toluene, acetone, xylenes and ethyl-benzene. Gasoline was found in 14%. A small amount of 1, 4-dioxane was detected as an additive in every 1, 1, 1, -trichloroethane products. With regard to reagents and others (n=53), the avarage number of components per sample was 1.40 if samples containig gasoline were excluded, and most (73%) of reagents and others (n=41) consisted of a single component. The potent toxic solvents such as benzene, chloroform, carbon tetrachloride, 1, 2-dichloroethane, 1, 1, 2-trichloro-ethane, 1, 1, 2, 2-tetrachloroethane and carbon disulfide were only rarely found in the present homogeneous products.

Carp plasma lipoproteins under fed condition were fractionated by sequential ultracentrifugation into VLDL (d

The method of integral geometry is developed to estimate fish density. We can estimate two dimensional measure using measurement of one dimensional quantity. In this papar, we apply this method called line intercept method. The conditions using this method are as follows: searching individuals by observer, numrous readily visible individuals, large individuals and interception of object by line. In this research, it was recognized that the use of an echo sounder as a searching observer is good for the line intercept method. There are some problems in estimating biomass, but the method is a good way to estimate number of fish schools. We think this method may be used widely in the future, though it has not yet been put sufficiently into practical use in the field of fisheries.

Poly (ethylene glycol) (PEG) has widely been used as a nonionic surfactant for dispersion, solubilination, and dehydration of protein or lipid. Recently, PEG is used fordfsf purification or concentration of biological substances such as viral suspenlion. Sensitive method is required for the measurement of PEG in the presence of impurities since the biological substances are usually contained large amounts of proteins or lipids. By Stevenson's method which is widely used for the measurement of PEG, it was difficult to measure the PEG in the presence of protein or lipid because nonspecific color development occurred by impurities. In this paper, we determined the substances which caused the nonspecific color development and found the procedure which removed them from samples. We described also more sensitive procedure for the measurement of PEG by the modification of concentration of chemical reagents which are used in Stevenson's method.

サイレージの乾物含量および埋蔵中の最高環境温度が開封後の好気的変敗の生起に及ぼす影響を知るために,4回にわたって,イタリアンライグラス予乾草を材料として実験を行なった.各実験とも,細切した予乾草を,そのまま(D),および水を20%添加して(W),バッグサイロに詰め,それぞれを2群に分けて,終始室温に(H),あるいは夏季(7月4日～9月11日)は22°Cの恒温室に(L)貯蔵した.H区の7,8,9月における平均室温は26.3°C,25.5°C,25.4°Cであり,最高室温が30°Cを越える日は16日あった.120～160日の埋蔵後に,サイレージを発泡スチロール製の断熱容器に移し,表面を空気にさらして25°Cの室内に7日間放置して変敗の生起を観察した.その結果,実験1(Dサイレージの乾物57.4%)と実験2(同じく51.7%)のD区では,好気的条件に放置した後の酵母数が多く,温度上昇が早期に起こり,2～3日後にピークが認められて,一且低下した後にふたたび温度の上昇が認められた.ところが,W区では温度上昇の開始が遅くなった.これに反して,実験3(Dサイレージの乾物47.0%)と実験4(同じく33.9%)では,一般に発熱開始が実験1,2よりも遅く,かつ,W区よりもD区の方が7日放置後の酵母数が少なく,発熱開始が遅かった.また,全実験を通じて,一般に埋蔵中の最高環境温度が高い方が,好気的条件における発熱も遅くなる傾向が認められた.

黄熟期および完熟期のとうもろこしサイレージを用い,サイロ開封後,対照区とプロピオン酸100mmol/kg添加区に分けて,断熱容器に詰め,23°Cに調温した呼吸試験用チャンバー内に,処理区ごとに好気的条件で6日間放置し,この間の品温,ガス代謝量,熱発生量,pHおよび浸透圧の変化,ならびに微生物相の遷移を調べた結果,次の知見を得た.1) サイレージ品温の変化は,ガス代謝量および熱発生量の経時的推移と相互によく対応した.発熱が生起した状態の呼吸商は,1に近似し,変敗が,微生物呼吸による炭水化物の完全酸化に伴う現象であることが推測された.2) 変敗の発現を判定する方法の一つとして,水抽出液浸透圧値の変化をみることが有効であることを認めた.3) 対照区の初期発熱および添加区の発熱には,酵母が関与した.しかし,優占種は材料により異なった.発熱ピーク時の酵母の生育期は,対数期の後期と静止期との中間に位置した.4) 対照区の細菌の増殖は,酵母が増殖する際に,有機酸を消費することにより誘発される可能性が推察された.5) 添加区の酵母フローラの遷移は,各菌種間ならびに菌種内細胞間のプロピオン酸抵抗性の差によって生ずると考えられた.6) 変敗により6日間にサイレージから消失する熱量は,乾物1kg当り対照区で1.53～2.64MJ,添加区で0.31～1.40MJであった.また,対照区の全発生熱量に占める初期発熱の割合は,約43%であった.

In occupational lead poisoning neurological symptoms, especially minor degrees of peripheral nerve damage, seem to be still common, although severe one such as lead palsy is now rare in clinical practice. Neurological and electrophysiological investigations were performed in 59 workers handling lead in 5 factories. The duration of the exposure was from less than one year to 43 years. Their age distribution was 18 to 64 years, with a mean of 38.4 years. Most of them had some of such complaints as joint pain, lumbago, muscle pain, dizziness, fatigue of lower limbs and so forth. Various nerve conduction measurements were done at median and tibial nerve segments of one side for every worker. Routine electromyography revealed neurogenic changes such as slight decrease in NMU, polyphasic potentials and occasional giant spikes in 39 cases. MCV reduced in 6 cases in the median nerve (*) and 2 cases in the tibial nerve. Sensory nerve conduction velocity was reduced in 8 cases in the median nerve. In 2 cases no sensory action potential could be recorded from the median nerve. Mixed nerve conduction velocity was significantly decreased in 11 cases for the median nerve segments (*) and im 9 cases for the tibial nerve segment.The results mentioned above suggested that there was an evidence of abnormality of peripheral nerve functions in the lead workers without marked clinical neurological signs. Besides, present electrophysiological examination revealed moderate disturbances not only of the sensory nerves but of the motor nerves in most cases. There did not appear any association between the electrical findings and urine lead levels or length of exposure to lead. ((*) The meam conduction velocities of both nerves were significantly lower in the group of lead workers as compared with the age-matched control group. p

One of the good fishing grounds for the round herring is off the western coast of the San'in district. Other main fishing grounds along the coast of San'in district lie around Oki islands and off the coast of Kyushu. A comparison of the relative abundances at two stages, the juvenile and the spawner, was made in order to understand the population dynamics of the round herring that dwells off the western coast of Shimane Pref. The results indicate that the relationship between the Parent and the Progeny well fits to a density dependent reproduction curve. It was, also, found that this species spawns from May to June off Hamada and consists of several subpopulations that are found off the coast of San'in district. The increase in mean fork length over the fishing season, based on measurements, give a growth curve of the round herring. Round herrings off the western coast of San'in district grow up 17cm in mean fork length at the end of the year of birth.

The surface pressures and the surface viscosities of lecithin, cephalin and its analogs were measured at the air—water and the oil—water interfaces. It was found that the surface viscosity of the phospholipids used in this study was very high, and was comparable to those of some polymer films at the oil—water interface as well as at the air—water interface under the conditions where the monolayers were condensed. The plateaus indicating the phase transitions in monolayers were clearly observed on the pressure-area curves at the oil—water interface in all of the specimens studied. It was found that the phase transitions exactly corresponded to the abrupt increases in surface viscosity. From the results thus obtained, an intermolecular ionic linkage between neighboring molecules in the monolayers is discussed.

The flight surveys were conducted five times during the day in the waters off the southeast coast of Hokkaido where a big catch of sardine Sardinops melanosticta has been taken. In the area sardine schools were easily sighted, but were only distinguished by dark color against lighter background of the sea. To examine a moving direction of school, an experiment was conducted near the fishing ground. In the experiment, it was carried out that floaters fixed to a seamarker were airdropped near a school, and after relative changes between the school and the seamarker were compared each other. The visual observations were conducted 4 times around the fishing ground. The shape of the schools was observed by sight and the number of schools was counted.From the result of the experiment, it is concluded that the front of the school, which indicates the direction of movement, is dark blue color and the rear is watery blue color. That is, by observing the shape and tone or shade of color of a school, we can easily estimate the moving direction of schools., During the visual observations, elongate shape schools have been frequently observed. It is estimated that there were much continuous immigrations from the outside of the small fishing area during before and after the surveys.

Nephrotoxicity of some aromatic nitro-amino compounds were evaluated by urinary enzyme activities and renal histopathological changes. Male Fischer 344 rats were intraperitoneally injected with aniline, p-aminophenol, acetyl-p-aminophenol, p-chloroaniline, p-chloronitrobenzene, p-anisidine, or p-nitroaniline at 1.0 mmol/kg. In the rats injected with p-aminophenol, necrosis of renal tubular epithelial cells and remarkable elevation of urinary N-acetyl-beta-D-glucosaminidase (NAG) and gamma-glutamyltranspeptidase (gamma-GTP) activities were observed. Injection with p-chloroaniline caused significant elevation of the urinary NAG and gamma-GTP activities. p-Anisidine and p-nitroaniline induced swelling of the tubular epithelial cells and a significant elevation in urinary NAG activities in rats, which was also caused by p-chloronitrobenzene. However, administration of aniline or acetyl-p-aminophenol did not change either the urinary enzymes or renal histopathology. These results indicate that p-aminophenol is a highly nephrotoxic substance, and that nephrotoxicity of p-chloroaniline, p-chloronitrobenzene, p-anisidine and p-nitroaniline exceed that of acetyl-p-aminophenol which has been known to cause a renal damage.

リン中毒動物の肝臓は著しく脂肪分が増加するといわれているが, ネコいらず中毒家兎の肝臓をクロロホルム, メタノールで抽出し, 溶剤による分画を行った。中毒のものはアセトン可溶の脂質が多いが, リン脂質分は乾燥肝臓粉末の5.4%で, 健康なものの7.4%より少ない。またリン脂質分画の中のモノアミノホスホグリセリドを主とするメタノールとエーテルに可溶な分画はともに上記の原料の3.4%でレシチン, ホスファチジルセリンおよびホスファチジルエタノールアミンが混在している。

Lauryl sarcosinate (L) -egg albumin (EA) -and lauryl sarcosinate-bovine serum albumin (BSA) complexes (EL, BL) were prepared by mixing each component dissolved in water at pH 7.07.5, respectively, Each complex comprising 10 mg of protein in the ratio L/protein=3 was twice injected in rabbits with Freund's Adjuvant method followed by thrice subcutaneous injections with 1 mg of each complex comprising 1 mg of protein in the ratio of L/protein=3, respectively. According to the ordinary precipitin reaction, we found only 1 or 2 positive reactions both in EAanti EL-and BSA-anti BL systems against 3 or 4 positive reactions in EA-anti EA-and BSA-anti BSA systems. On the other hand, we found only 1 positive reactions in the cases of EL-anti-EL-and BL-anti BL systems, respectively. The titers of antibody were 1 : 1 or 1 : 4 in EA-anti EL-against 1 : 64 or 1 : 128 in that of EA-anti EA systems, and 1 : 4 or 1 : 8 in BSA-anti BL-against 1 : 32 in BSA-anti BSA systems. Arthus phenomenon showed moderate reaction for BSA-anti BL-and severe reaction (necrose) in BSA-anti BSA systems.

Human plasma lipoproteins (high density lipoprotein, low density lipoprotein) are prepared by ultracentrifugal method. Followed delipidation of these lipoproteins is performed with ether containing SDS to obtain their apoproteins. These lipoproteins and apoproteins are subjected to ultrasonic irradiation (20 kc) to accelerate the deterioration. The UV-spectra of ultrasonically irradiated preparations generally increase, and the maximum absorption shifts to the shorter wavelength by ultrasonic irradiation. Similar changes of UV-spectra are observed between ultrasonically irradiated lipoproteins and the lipoproteins stored for long term. In this paper the authors discussed about the influences of ultrasonic irradiation on lipoproteins.

Crude high density lipoprotein of egg yolk was fractionated by rate zonal ultracentrifugation. Rate zonal ultracentrifugation was carried out with zonal rotor RPZ-43T (660ml) in density gradient d. 1.00-1.40 by sodium bromide at 40000rpm.By the ultracentrifugation for four hours, the separation of high density lipoprotein (HDL) from low density lipoprotein (LDL) was observed, but HDL was completely purified only by the second ultracentrifiugation for twenty four hours after the first procedure.The purified HDL shows only one peak in the schlieren pattern of the analytical ultracentrifugation.