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Your search keyword '"Hsieh, Yi‐Hsien"' showing total 672 results
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672 results on '"Hsieh, Yi‐Hsien"'

108. Melatonin combined with sorafenib synergistically inhibit the invasive ability through targeting metastasis-associated protein 2 expression in human renal cancer cells.

Author

Lee, Chu-Che, Huang, Po-Yu, Hsieh, Yi-Hsien, Chen, Yong-Syuan, and Tsai, Jen-Pi

Abstract

Objectives: Renal cell carcinoma (RCC) was the most common and lethal urological malignancy with the dismal outcome when distant metastasis. Melatonin was known as a potential oncostatic agent against several types of malignancy and sorafenib had been considered as an agent to treat RCC, but the synergistic effects of melatonin and sorafenib on human RCC have not been elucidated. Materials and Methods: Human renal cancer cell lines (Caki-1 and ACHN) were treated with melatonin combined with sorafenib were detected the cell growth and cell cycle by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and flow cytometry. The ability of cell migration/invasion was performed with in vitro migration and invasion assay. The proteins and mRNA expression of metastasis-associated protein 2 (MTA2) from the RCC cells were measured by quantitative reverse transcription-polymerase chain reaction and western blotting. Clinical significance of MTA2 in RCC tissues was analyzed from The Cancer Genome Atlas database by using TISIDB software. Results: Our results showed that melatonin combined with sorafenib, sorafenib or melatonin-treated alone did not induce the cytotoxic effects or cell cycle arrest in human RCC cells and HK2 cells. Additionally, cotreatment with melatonin and sorafenib synergistically reduced migration and invasion in human Caki-1 and ACHN cells through synergistically suppression of MTA2 expression. Bioinformatics analysis showed that MTA2 expression significantly correlated with overall survival (P < 0.002), tumor grade (P < 0.001) and tumor stage (P < 0.001) in human RCC. Conclusion: Our results demonstrated that concomitantly used melatonin and sorafenib could significantly reduce the abilities of migration and invasion of RCC cells through inhibiting MTA2. We considered that this novel promising combination strategy towards the treatment of RCC, but further studies are warranted. [ABSTRACT FROM AUTHOR]

Published
2022
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138. Praeruptorin A reduces metastasis of human hepatocellular carcinoma cells by targeting ERK/MMP1 signaling pathway.

Author

Yu, Chen‐Lin, Yu, Yung‐Luen, Yang, Shun‐Fa, Hsu, Chung‐En, Lin, Chia‐Liang, Hsieh, Yi‐Hsien, and Chiou, Hui‐Ling

Subjects
HEPATOCELLULAR carcinoma, HUMAN cell cycle, METASTASIS, CANCER cell migration, HUMAN beings, CELL cycle
Abstract

Praeruptorin A (PA) is one of the active ingredients found in the dried root of Peucedanum praeruptorum Dunn, has been reported to possess anticancer effects against various types of cancer. However, the effect of PA on human hepatocellular carcinoma (HCC) remains uncleared. In this study, our results indicated that PA did not induce cytotoxicity or alter cell cycle distribution in human HCC cells (Huh‐7, SK‐Hep‐1, and PLC/PRF/5 cells). Instead, PA inhibited the migration and invasion of human HCC cells while downregulating the expression of matrix metalloproteinase‐1 (MMP1) and activating the extracellular signal‐regulated kinase (ERK) signaling pathways. Furthermore, blocking the ERK signaling pathway through siERK restored the expression of MMP1 and the invasive ability of PA‐treated HCC cells. In conclusion, our results demonstrate the antimetastatic activity of PA against human HCC cells, supporting its potential as a therapeutic agent of HCC treatments. [ABSTRACT FROM AUTHOR]

Published
2021
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139. Arecoline induces epithelial mesenchymal transition in HK2 cells by upregulating the ERK‐mediated signaling pathway.

Author

Hsieh, Yi‐Hsien, Syu, Ru‐Jiang, Lee, Chu‐Che, Lin, Shin‐Huey, Lee, Chien‐Hsing, Cheng, Chun‐Wen, and Tsai, Jen‐Pi

Subjects
EPITHELIAL-mesenchymal transition, BETEL nut, RENAL fibrosis, WESTERN immunoblotting, CHRONIC kidney failure, EXTRACELLULAR signal-regulated kinases
Abstract

Arecoline, a component of betel nuts, is a known carcinogen that causes oral cancers among those who chew betel nuts. Betel nut chewing is also associated with an increased risk of chronic kidney disease (CKD), but the role of arecoline in this association is unclear. This in vitro study investigates the effects of arecoline on cultured human kidney (HK2) cells. We observed that arecoline had no effect on cell viability but increased cell migration in a dose‐dependent manner. Western blot analysis showed that arecoline treatment caused a dose‐dependent decrease in E‐cadherin expression and dose‐dependent increases in N‐cadherin, vimentin, α‐SMA, and collagen expression; reverse transcriptase‐polymerase chain reaction analysis revealed dose‐dependent increases in α‐SMA and collagen mRNA. Arecoline treatment upregulated the expression of phosphorylated extracellular signal‐regulated kinase through epithelial mesenchymal transition and renal fibrosis in HK2 cells. These findings demonstrate that arecoline plays a role in inducing the epithelial mesenchymal transition and fibrogenesis in renal tubule cells and suggest that arecoline promotes the progression of CKD. [ABSTRACT FROM AUTHOR]

Published
2020
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