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CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico L-(+)-Bornesitol é o constituinte majoritário do extrato etanólico de folhas de Hancornia speciosa Gomes (Apocynaceae), planta medicinal com atividade anti-hipertensiva comprovada. O presente trabalho teve como objetivo validar L-(+)-bornesitol como marcador ativo de H. speciosa, empregando estudos de farmacodinâmica e farmacocinética em ratos, e de permeação in vitro. O bornesitol foi isolado de um extrato em metanol / acetato de etila (1:1) de folhas de H. speciosa e sua pureza e identidade foram aferidos por métodos espectrométricos usuais (RMN de 1H e de 13C e MS). Para a realização dos estudos farmacocinéticos desenvolvemos e validamos um método bioanalítico para quantificar o bornesitol em plasma de rato por cromatografia líquida de ultra eficiência acoplada a espectrômetro de massas sequencial com ionização por electrospray (UPLC-ESI-MS/MS). O efeito hipotensor do bornesitol foi investigado em ratos Wistar normotensos. Bornesitol (0,1; 1,0 e 3,0 mg/kg i.v.) promoveu a redução da pressão arterial sistólica (PAS) e pressão arterial média (PAM) dos animais e seu mecanismo de ação foi caracterizado como resultante da inibição da atividade da enzima conversora de angiotensina e aumento da concentração de óxido nítrico. O método bioanalítico de UPLC-ESI-MS/MS/MS desenvolvido e validado utilizou modo de aquisição por Monitoramento de Reações Múltiplas (MRM) e ionização em modo negativo, e foi baseado em duas transições observadas para o analito, uma específica (m/z 193,1 → 161,0 Da) e outra confirmatória (m/z 193,1 → 125,0 Da). Pentaeritritol foi definido como padrão interno, sendo duas transições empregadas para sua análise, uma específica (m/z 135,1 → 87,3 Da) e outra confirmatória (m/z 135,1 → 69,3 Da). Para a realização do estudo farmacocinético, o plasma foi purificado por meio da precipitação de proteínas com acetonitrila. O método mostrou-se linear, exato, preciso, específico e sensível, sendo adequado para a determinação dos perfis plasmáticos do composto após diferentes doses e vias de administração. O bornesitol (3, 15 e 25 mg/Kg) administrado a ratos Wistar apresentou perfil farmacocinético não linear e com modelo bicompartimental. A permeabilidade do bornesitol foi avaliada in vitro em modelo transwell com células Caco-2. Quando avaliado isoladamente, o bornesitol apresentou baixa permeação, provavelmente devido à elevada hidrofilia do composto, de maneira semelhante à relatada para outros ciclitóis. A permeabilidade do bornesitol foi aparentemente aumentada quando este foi administrado associado com a rutina, ou como constituinte da matriz de uma fração padronizada de H. speciosa.

Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq Atualmente, a busca pelo uso integral dos resíduos da biomassa vegetal, tem sido considerada necessária na atenção ao meio ambiente, bem como a redução de custos e a valorização dessas matérias-primas. Entre esses materiais estão os resíduos agroindustriais amplamente empregados para a obtenção de diversos produtos como o óleo, extratos, enzimas, entre outros. Este estudo investigou o uso do resíduo despolpado de mangaba (Hancornia speciosa Gomes) que foi caracterizado, destacando-se o seu teor de óleo, e utilizando-o como substrato para a produção de enzimas (lipase e pectinases) por fermentação em estado sólido (FES) utilizando o fungo filamentoso Aspergillus niger IOC 4003. O resíduo de mangaba e seus extratos etanólicos foram avaliados quanto aos aspectos físico-químicos e bioativos, destacando-se principalmente, o teor de fenólicos totais e a capacidade antioxidante. Dentre as enzimas avaliadas, a poligalacturonase (PG) foi submetida à ensaios de estabilidade sob diferentes condições de temperatura e pH e aplicada na concentração de 1,0% no processo de clarificação do suco de mangaba. Destaca-se que o resíduo pós-fermentação foi usado para extração de lignina que foi aplicada na obtenção de biofilme de quitosana, avaliando-se a capacidade antioxidante destes bem como sua capacidade para aumentar a vida de prateleira do mamão papaia (Carica papaya) após o recobrimento. Os resultados mostraram que resíduo de mangaba possui atividade antioxidante com concentrações de compostos fenólicos totais de 564,1 ± 0,047 mg de equivalente de ácido gálico/g de óleo extraído e Atividade Antioxidante Total - TCA de 95,46 ± 1,58 mg de equivalente de ácido ascórbico/g de resíduo. Além disso, por FES foi possível produzir de lipases (50,11 ± 0,02 U/g), pectinases totais (0,45 ± 0,01 U/g) e poligalacturonases (76,32 ± 0,11 U/g). O extrato apresentou estabilidade à atividade de PG acima de 80% para temperaturas de 20 e 25 ° C, mantendo este nível de atividade por até 1 hora. No entanto, com redução significativa em temperaturas superiores a 40 °C. Em relação ao pH, a atividade da PG foi consideravelmente reduzida em pH alcalino e, em pH 4 e 5, a PG manteve sua atividade relativa acima de 50% por até 120 minutos. Quando aplicado com carga enzimática de 1,0 U/mL o extrato enzimático apresentou resultados satisfatórios no processo de clarificação do suco de mangaba permitindo uma redução de 34% na turbidez e 11% de viscosidade. Os biofilmes de quitosana a 2% adicionados com 0,1 % e 0,05% (m/m) de lignina alcalina apresentaram atividade antioxidante, com base na captura do radical DPPH. As frutas recobertas com as soluções filmogênicas tiveram suas vidas de prateleira aumentadas. Dessa forma, o resíduo de mangaba apresenta-se como matéria-prima de baixo custo e com potencial para a produção de produtos biotecnológicos de interesse para a sociedade. Currently, the search for the full use of plant biomass residues has been considered necessary in the attention to the environment, as well as the reduction of costs and the valorization of these raw materials. Among these materials are agro-industrial waste widely used to obtain various products such as oil, extracts, and enzymes, among others. This study investigates the use of the characterized mangaba waste (Hancornia speciosa Gomes), highlighting its oil content, and using it as a substrate for the production of enzymes (lipase and pectinases). Solid-state fermentation (FES) using the filamentous fungus Aspergillus niger IOC 4003. Mangaba residue and its ethanolic extracts were evaluated for physicochemical and bioactive aspects, especially the total phenolic content and antioxidant capacity. Among the evaluated enzymes, polygalacturonase was submitted to stability tests under different temperature and pH conditions and applied at a concentration of 1.0% in the clarification process of mangaba juice. It is noteworthy that the post-fermentation residue was used for lignin extraction that was applied to obtain chitosan biofilm, evaluating their antioxidant capacity as well as their capacity to increase the shelf life of papaya (Carica papaya) after coating. The results showed that mangaba residue has antioxidant activity with total phenolic compound concentrations of 564.1 ± 0.047 mg gallic acid equivalent / g extracted oil and Total Antioxidant Activity - TCA of 95.46 ± 1.58 mg equivalent of ascorbic acid / g residue. Furthermore, by FES it was possible to produce lipases (50.11 ± 0.02 U / g), pectinases (0.45 ± 0.01 U / g) and polygalacturonases (76.32 ± 0.11 U / g). The extract showed stability to PG activity above 80% for temperatures of 20 and 25 ° C, maintaining this activity level for up to 1 hour. However, with significant reduction at temperatures above 40 ° C. In relation to pH, PG activity was considerably reduced at alkaline pH and, at pH 4 and 5, PG kept its relative activity above 50% for up to 120 minutes. When applied with enzymatic load of 1.0 U / mL, the enzymatic extract presented satisfactory results in the clarification process of mangaba juice allowing a 34% reduction in turbidity and 11% viscosity. The 2% chitosan biofilms added with 0.1% and 0.05% (w / w) alkaline lignin showed antioxidant activity, based on the capture of the DPPH radical. The fruits coated with filmogenic solutions had their shelf lives increased. Thus, mangaba waste is presented as a low cost raw material with potential for the production of biotechnological products of interest to society.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Hancornia speciosa Gomes (mangabeira) is used in folk medicine to treat different diseases, including inflammatory conditions. Leaves and fruits have been extensively studied but there is no investigation on the biological effects of the fixed oil extracted from its seeds. The aim this study was evaluated the anti-inflammatory activity of the oil from “mangaba” seeds (OMS) in mice. Adult male Swiss mice (25-30 g) were used and the protocols were approved by the Ethics Committee for Animal Research (n°28/2018). Cytotoxicity was evaluated in fibroblasts of the L929 cell line through MTT assay. The topical anti-inflammatory activity was evaluated in the ear edema induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). Treatment with OMS (0.3, 1.0 or 3.0 mg/ear) or dexamethasone (0.05 mg/ear, control) was concomitantly administrated with TPA (1 µg/ear) in the ipsilateral ear, topically. After 6 hours, edema, myeloperoxidase (MPO) activity, cytokine levels (TNF-α, IL-6 and IL-1β), histological alterations, vascular permeability and oxidative parameters were measured. The topical effect has also evaluated in the capsaicin-induced ear edema (0.2 mg/ear). In this model, animals were pre-treated with OMS (3 mg/ear) or ruthenium red (7.8 ng/ear, control) administrated in the ipsilateral ear 15 minutes before induction. After 30 minutes was measured ear edema. For evaluation of the systemic anti-inflammatory activity, mice were treated with vehicle (Tween 0.5% in saline, 10 mL/kg), OMS (100, 200 or 400 mg/kg, p.o. or i.p.) or dexamethasone (5 mg/kg, p.o. or s.c.) administered 1 hour or 30 min, respectively (for each route of administration), before intrapleural injection of carrageenan (1 mg/cavity). The fluid leakage from the pleural cavity was collected 4 h after induction for assessment of total leukocyte counts, MPO activity, protein extravasation and cytokine levels (TNFα, IL-6, IL-1β and IL-10). Results were expressed as mean ± S.E.M. and were evaluated by one-way ANOVA followed by Tukey's test, with p

O objetivo deste estudo foi avaliar, em viveiro, o desenvolvimento de mudas de H. speciosa oriundas de diferentes áreas do Estado de Goiás. Nestas áreas foram selecionadas 116 plantas, coletando-se 12 frutos por planta para obtenção das sementes. Uma amostra de 16 sementes representadas por cada matriz teve duas sementes semeadas por tubete e mantidas em viveiro; e destas, as que originaram plântulas, foram submetidas a avaliações de comprimento (cm) e número de par de folhas (NPF). Os dados foram coletados mensalmente para cada progênie até a décima (10ª) avaliação. Foram realizadas análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. As mudas praticamente estabilizam seu crescimento entre maio e setembro. A taxa média mensal de crescimento variou de 0,1 cm a 6,1 cm, com taxa média final de 2,19 cm. É oportuno pesquisar alternativas de produção de mudas em períodos menores., Revista de Ciências Agrárias, vol. 36 n.º 3 (2013)

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Resumo Objetivo Este trabalho objetivou avaliar o efeito sistêmico do látex de H. speciosas obre a neoformação óssea. Métodos Para isso, o látex foi coletado e diluído a 3% e a 50%. Um total de 28 ratos Wistar foi submetido a cirurgia para a criação de um defeito de 5 mm de diâmetro no osso parietal. Esse experimento foi conduzido em dois períodos distintos: 1 e 2. Para cada período, os ratos foram divididos em 3 grupos: Grupo Controle, Grupo Látex3 e Grupo Látex50 que receberam, respectivamente, administrações diárias de 0,5 mL de água destilada, látex a 3% e látex a 50% por gavagem, via oral. Os ratos dos períodos 1 e 2 foram eutanasiados, respectivamente, 15 e 30 dias após a cirurgia e a calvária foi coletada. Os resultados foram analisados utilizando os testes ANOVA e Tukey; o nível de significância estabelecido foi 0,05. Resultados Mostramos que, em cada período analisado, os grupos experimentais tiveram a mesma quantidade de osso neoformado no defeito da calvária. Conclusão Portanto, concluímos que administrações diárias e orais do látex de H. speciosa a 3% e a 50% durante um período de 15 e 30 dias não contribui para o aumento da área do osso neoformado no defeito da calvária.

The Mangaba (Hancornia speciosa Gomes) is a fruit tree rich in such vitamins, iron, calcium and protein, and plays an important social, cultural and economic role for farmers in the Cerrado and Caatinga biomes of Brazil. The aim of this study was to estimate genetic parameters and genetic gains in the agrotechnological attributes of a breeding population of the mangaba from Embrapa Meio Norte. Made available in DSpace on 2019-12-09T18:18:04Z (GMT). No. of bitstreams: 1 Repeatabilityestimation5758228341PB.pdf: 379893 bytes, checksum: ecb745b59a78222c5bfb334d81db9ea8 (MD5) Previous issue date: 2019

This study aimed to conduct an ethnobotanical survey of medicinal species in an area of cerrado vegetation in the chapada do Araripe, Ceara, as well as evaluating and selecting species with potential for bioprospecting, based on the relative importance of the species and the consensus among the informants. The ethnobotanical data were collected through semi-structured interviews and a free list, and by using the technique "snowball". It was analyzed the relative importance of the mentioned plants as well as species that stood out on the bodily systems, based on the factor:consensus among the informants. It was recorded a total of 38 species distributed into 35 genera and 25 families. Of the total eight species (21%) presented great versatility (IR≥1) regarding to its use. The therapeutic indications were grouped into 14 categories of bodily systems. Disorder of respiratory and genitourinary systems, injuries, poisoning and other consequences of external causes and diseases of the blood, organs and connective tissue, obtained a greater consensus among the informants. Ximenia americana, Himathantus drasticus, Stryphnodndron rotundifolium, Hancornia speciosa and Cecropia pachysthachya were the species subject to further study, based on versatility, and consensus of the use among the informants. Key words: Ethnobotany, factor in consensus, local knowledge, potential therapeutic, regional species, relative importance.

The mangaba (Hancornia speciosa Gomes) is a typical fruit of the Brazilian cerrado and caatinga. It has excellent nutritional composition but there are no studies reporting the fruit characteristics and its industrial use. The aim of this study was to analyze the physical and physicochemical characteristics of mangaba fruit in three different maturity stages. Fruit weight, volume, length, titratable acidity, vitamin C, soluble solids and color at 1/3 ripe, 2/3 ripe and ripe maturity stages were analyzed. Physical characteristics showed great potential for industrialization due to the size and weight of fruits. High vitamin C levels and low acidity indexes were obtained, which are of interest for use of fruits in jams, jellies and liquors. However, soluble solids content was lower for ripe fruits, which indicates low sugar contents. Thus, the fruit has potential for consumption in the natural form and for processing and can be incorporated into many food products. Key words: Maturation, composition, economic potential.

Ethnobotanical surveys have shown that the stem bark of Hancornia speciosa Gomes (Apocynaceae) is popularly used to treat obesity and diabetes. However, there is no experimental evidence that confirms such use. The present study investigated the effects of the aqueous extract of the stem bark of H. speciosa (AEHS) on the glycemic and adipogenic profiles of obese mice. Mice were divided into four groups that received standard diet (SD), standard diet plus AEHS (SDE), high-fat diet (HD) and high-fat diet plus AEHS (HDE). The administration of AEHS (in a concentration of 0.3 mg.mL-1. ad libitum in the drinking water) was performed for the last 8 weeks totaling a period of 18 weeks, in which the animals received the diets. Whole body weight, liquid intake and food consumption were measured during the entire experiment. Blood glucose levels, insulin sensitivity, glucose tolerance and adipose pads weight were evaluated. Animals from the HD group presented higher body weight in comparison to animals from the SD group. That was associated with insulin resistance and glucose intolerance, as well as increased blood glucose levels (p

Hancornia speciosa is a promising fruit species due to the high protein content of its fruit. However, there is little information about its production, with a lack of information concerning the germination of its seeds and initial seedling growth. The aim of this study was to evaluate water availability and suitable substrates for its seedling emergence and production. The seeds were deposited in tubes, containing the following substrates: Distroferric Red Latosol (L); Latosol+Sand (L+A); Latosol+Bioplant (L+B); Sand+Latosol+Poultry Litter in the following proportions 2:2:1 (L+A+C1) and 2:4:1 (L+A+C2) associated with water retention capacities of 25%, 50%, 75% and 100%. The substrates L+A, L+B and L and a water availability of 75% to 100% are recommendable for seedling emergence and early seedling growth. Substrates that consisted of poultry litter and water availability of 25% and 50% were prejudicial to growth and quality of Hancornia speciosa seedlings.

Mangabeira (Hancornia speciosa Gomes), a fruit tree native to the Brazilian Cerrado, has great potential for commercial cultivation due to the quality of fruits, which can be consumed fresh or processed. However, it is necessary to expand research in the area of propagation of this fruit tree. The aim of this study was to evaluate different substrates and container volumes in the production of mangabeira seedlings, H. speciose, in Nova Xavantina-MT. The experiment was carried out at the nursery of the Mato Grosso State University (UNEMAT), Campus of Nova Xavantina in a randomized block design, in a 2 x 4 factorial scheme, with two container volumes (0.9 e 1.8 dm3) and four substrates (soil; soil + sand; soil + sand + bovine manure; and commercial substrate), totalizing eight treatments with four replicates and plots composed of 10 plants each. The greatest growth of mangabeira seedlings was observed using soil or soil + sand + bovine manure and 1.8 dm3 container.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Sergipe produces annually near 352 tons of Mangaba (Hancornia speciosa), and is the chief mangaba’s producer in Brazil. Most part of this production is industrialized as frozen pulp, being this activity very important to the state’s economy. However, mangaba’s industrialization generates wastes that if not properly processed, cause environmental problems. A considerable amount of mangaba’s agroindustrial waste consists of seeds showing high lipid content. The aim of this work was to evaluate the potential of the seeds from mangaba’s agroindustrial waste, to produce oil for culinary and/or cosmetic applications. Mangaba’s seeds obtained from an agroindustry located in Aracaju/SE, were dried, ground and submitted to cold pressing in an hydraulic press under 30 tons. The oil obtained was characterized regarding its relative density, moisture content, indexes of refraction, acidity, saponification and iodine, and fatty acid profile. The volatile compounds of the oil’s headspace were identified in comparison to the processed pulp. They were isolated through a solid phase microextraction technique (SPME), separated both in an HP-5MS and in a DB-Wax capillary column, and analyzed in a GC-MS system. The isolation conditions were optimized using a factorial design 2 2 (DCCR), whose independent variables were the adsorption temperature (40ºC- and 80ºC) and the extraction time (20 – 60 min). The data were evaluated by Response Surface Methodology and Desirability Function. Trained judges generated the oil’s aroma profile in comparison to three commercial cold pressed oils, through quantitative descriptive analysis (QDA). The sensory data were evaluated by ANOVA, Tukey (p≤0,05) and Principal Component Analysis (PCA). The results showed that the oil extraction process from the mangaba’s seeds containing 4,7% humidity and 16,7% of lipid, presented 37% yield. The extracted oil showed relative density of 0,91 g.cm-3, acidity of 5,47 mgKOH.g-1, saponification index of 193,00 mgKOH.g-1 and iodine value of 69 cgI2.g-1. The oil´s fatty acid profile was similar to the olive oil; it contained 72% of unsaturated fatty acids and oleic acid as the major fatty acid (63,7%). Among the tocopherols, only α-tocopherol was identified in the oil; its concentration was 6,17mg/100g. The best isolation condition of the oil´s volatiles was predicted as 57,2ºC and 60 min of fiber exposition. Eighty (80) volatiles were identified in the oil and 82 in the processed pulp. Esters formed the major chemical class in the oil, as well as in the pulp, corresponding to respectively 56,7% and 70,9% of the total area of the chromatogram. The alcohols were the second major chemical class representing 13,9% and 18,4% of the total chromatogram area of the oil and the processed pulp, respectively. The mangaba seed’s oil presented a distinguished aroma which differed from the others cold pressed oils analyzed due to a higher intensity (p≤ 0,05) of notes described as mangaba’s seed, sweetness, frutal, freshness and caramelized banana. The results indicate that the mangaba’s seed is suitable for cold pressing oil extraction. Due to its similarity to the olive oil regarding their fatty acid profiles, and to its esters content, which are volatiles usually associated to desirable fruit aroma, the mangaba´s oil has potential to be used in culinary and by the cosmetic industry. However, complementary studies must be performed in order to ensure the oil safety with respect to its toxicity and allergenicity before its use in the suggested applications. Sergipe produz anualmente cerca de 352 toneladas de mangaba (Hancornia speciosa), sendo o maior produtor desse fruto no Brasil. Grande parte dessa produção é industrializada na forma de polpa congelada, atividade muito importante para a economia do Estado. Entretanto, a industrialização da mangaba gera resíduos que se não forem adequadamente tratados, provocam problemas ambientais. Uma parte considerável dos resíduos agroindustriais da mangaba é constituída pelas sementes do fruto, que apresentam alto teor lipídico. O objetivo do presente trabalho foi avaliar o potencial das sementes dos resíduos agroindustriais da mangaba para a produção de óleo destinado a aplicações culinárias e/ou cosméticas. Sementes de mangaba obtidas junto a uma agroindústria de Aracaju, SE, foram secas, moídas e submetidas à prensagem a frio em prensa hidráulica sob 30 toneladas. O óleo obtido foi caracterizado quanto à densidade relativa, teor de umidade, índices de refração, acidez, saponificação, iodo, e perfil de ácidos graxos. Os compostos voláteis presentes no “headspace” do óleo foram identificados comparativamente à polpa processada. Para o isolamento dos voláteis foi utilizada a técnica de microextração em fase sólida (SPME) utilizando a fibra DVB/Carboxen/PDMS. Os voláteis foram separados em colunas capilares HP-5MS e DB-Wax, e analisados em um sistema GCMS. As condições de isolamento dos voláteis foram otimizadas utilizando-se um delineamento fatorial 22 (DCCR), tendo como variáveis independentes a temperatura de adsorção ( 40ºC-e 80ºC) e o tempo de extração (20 - 60 min). Os dados foram analisados por Metodologia de Superfície de Resposta e função “Desirability”. Julgadores treinados geraram o perfil de aroma do óleo comparativamente a três óleos comerciais prensados a frio, utilizando análise descritiva quantitativa (ADQ). Os dados sensoriais foram analisados por ANOVA, Tukey (p≤0,05) e Análise de Componentes Principais (ACP). Os resultados indicaram que o processo de extração de óleo da semente de mangaba seca a 4,7% de umidade, e contendo 16,7% de lipídios apresentou rendimento de 37%. O óleo obtido apresentou densidade relativa de 0,91 g.cm-3, e índices de acidez de 5,47 mgKOH.g-1, de saponificação de 193,00 mgKOH.g-1, e de iodo de 69 cgI2.g-1. O óleo mostrou um perfil de ácidos graxos similar ao azeite de oliva, apresentando 72% de ácidos graxos insaturados, e o ácido oleico como majoritário (63,7%). Dentre os tocoferóis, apenas o α-tocoferol foi identificado no óleo, e em concentração de 6,17mg/100g. As melhores condições de isolamento dos voláteis presentes no óleo foram 57,2°C e 60 minutos de exposição da fibra. Foram identificados 80 compostos voláteis no óleo e 82 na polpa processada. Tanto no óleo quanto na polpa os ésteres foram os compostos majoritários, correspondendo a aproximadamente 56,7% e 70,9% da área do cromatograma das amostras, respectivamente. Os álcoois foram a segunda classe química majoritária, representando 13,96% e 18,4% da área do cromatograma do óleo e da polpa processada, respectivamente. O óleo de semente de mangaba apresentou aroma diferenciado dos demais óleos prensados a frio analisados, possuindo maior intensidade (p≤ 0,05) de aroma de semente de mangaba, doce, frutal, frescor e, banana caramelizada. Os resultados indicaram que a semente de mangaba apresenta viabilidade para extração de óleo por prensagem a frio. Adicionalmente, por apresentar perfil de ácidos graxos similar ao de azeite de oliva, e a presença de ésteres, voláteis usualmente associados a aromas desejáveis de fruta, o óleo obtido apresenta potencial de uso na culinária e indústria cosmética. No entanto estudos complementares devem ser realizados para comprovar a segurança alimentar e cosmética do produto, notadamente no que se refere a toxicidade e alergenicidade, antes das aplicações sugeridas. São Cristóvão

This work describes the potential of chemometric analyses applied to 1H high-resolution magic angle spinning nuclear magnetic resonance (1H HR-MAS NMR) data for the chemotaxonomic investigation of Hancornia speciosa (Apocynaceae) varieties. This plant, popularly known as mangaba, has a complex morphological differentiation and thus chemical analyses can be used for their taxonomic classification. In comparison to traditional techniques, 1H HR-MAS NMR allied with chemometrics provided a simple and low cost method for chemotaxonomy. Leaves of four varieties of H. speciosa from a common garden experiment was studied and demonstrated that H. speciosa var. speciosa differs from others due to its specific metabolic profile, and var. pubescens was discriminated based on its high phenolic compound content. The distinction between the latter variety and gardineri is important once it allows for the selection of samples with greater commercial value, once they produce the largest and heaviest fruits.

Submitted by Mario BC (mario@bc.ufrpe.br) on 2019-01-04T16:16:07Z No. of bitstreams: 1 Jackeline Terto da Silva Santana.pdf: 2037490 bytes, checksum: e72a7da0198dde8ae5de59573371caf9 (MD5) Made available in DSpace on 2019-01-04T16:16:07Z (GMT). No. of bitstreams: 1 Jackeline Terto da Silva Santana.pdf: 2037490 bytes, checksum: e72a7da0198dde8ae5de59573371caf9 (MD5) Previous issue date: 2018-03-05 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The genus Hancornia is monotypic whose species is called Hancornia speciosa Gomes (mangabeira). It is a fruit native to Brazil that plays a relevant role in the tropical fruit market. Six botanical varieties are described according to morphological differences: var. speciosa, var. pubescens, var. gardneri, var. maximiliani, var. lundii and var. cuyabensis. However, the difficulty in distinguishing them in natural populations suggests that these botanical varieties are variations due to ecological adaptations. In this sense, the construction of the phylogeny of the genus allows to elucidate the taxonomic classification. Among molecular methods, the use of standard regions (DNA barcoding) is characterized by being safe and promising in phylogenetic studies. This study aimed to study the phylogeny of the genus Hancornia using plastid sequences (trnH-psbA, rbcL and matK). Leaves were collected in the States of Sergipe, Pernambuco, Paraíba, Maranhão, Ceará, Bahia, Goiás, Minas Gerais and in the Germplasm Collection of the Federal University of Goiás. DNA was duly extracted and PCR reactions and sequencing of genome chloroplast were performed. The trnH-psbA intergenic region identified eleven haplotypes. The phylogenetic tree showed the formation of two clades: one for speciosa variety and one for pubescens, gardneri and cuyabensis, demonstrating that the latter are genetically closer. A similar result was obtained in the principal component analysis where it reveals that speciosa forms a distinct grouping of the other varieties studied. This fact may be a consequence of isolation by distance since this variety occurs only in the Atlantic Forest and Caatinga. Thus, the evolutionary relationship between the varieties evidences the formation of two genetic groups, one for speciosa and another for gardneri, cuyabensis and pubescens, and it was revealed that gardneri possibly a natural hybrid between cuyabensis and pubescens. In addition, the absence of the varieties maximiliani and lundii is shown, showing only variations due to the environment. Therefore, only three botanical varieties should be considered for H. speciosa: pubescens, cuyabensis and speciosa. O gênero Hancornia é monotípico cuja espécie se denomina Hancornia speciosa Gomes (mangabeira). Trata-se de uma frutífera nativa do Brasil que desempenha relevante papel no mercado de fruticultura tropical. São descritas seis variedades botânicas de acordo com diferenças morfológicas: var. speciosa, var. pubescens, var. gardneri, var. maximiliani, var. lundii e var. cuyabensis. No entanto, a dificuldade em distingui-las em populações naturais sugere que essas variedades botânicas constituam variações devido a adaptações ecológicas. Nesse sentido, a construção da filogenia do gênero permite elucidar a classificação taxonômica. Entre os métodos moleculares, o uso de regiões padrões(DNA barcoding) caracteriza-se por ser segura e promissora em estudos filogenéticos. Esta pesquisa teve como objetivo estudar a filogenia do gênero Hancornia usando sequências plastidiais (trnH-psbA, rbcL e matK). Folhas foram coletadas nos Estados de Sergipe, Pernambuco, Paraíba, Maranhão, Ceará, Bahia, Goiás, Minas Gerais e na Coleção de Germoplasma da Universidade Federal de Goiás. O DNA foi devidamente extraído e as reações de PCR e sequenciamento das regiões do genoma de cloroplasto foram realizadas. A região intergênica trnH-psbA identificou onze haplótipos. A árvore filogenética mostrou a formação de dois clados: um para a variedade speciosa e outro para pubescens, gardneri e cuyabensis, demonstrando que estas últimas são mais próximas geneticamente. Resultado semelhante foi obtido na análise de componentes principais onde revela que speciosa forma um agrupamento distinto das demais variedades estudadas. Este fato pode ser consequência de um isolamento por distância já que esta variedade ocorre somente na Mata Atlântica e Caatinga. Dessa forma, a relação evolutiva entre as variedades evidencia a formação de dois grupos genéticos, um para speciosa e outro para gardneri, cuyabensis e pubescens, sendo revelado que gardneri possivelmente é um híbrido natural entre cuyabensis e pubescens. Além disso, comprova-se a inexistência das variedades maximiliani e lundii, mostrando ser apenas variações devido ao ambiente. Portanto, somente três variedades botânicas devem ser consideradas para H. speciosa: pubescens, cuyabensis e speciosa.

Submitted by Mario BC (mario@bc.ufrpe.br) on 2019-02-11T12:50:58Z No. of bitstreams: 1 Vanessa Emanuelle Pereira Santos.pdf: 1410254 bytes, checksum: c10ced66b2b2a50acd99e2b5cb8651e8 (MD5) Made available in DSpace on 2019-02-11T12:50:58Z (GMT). No. of bitstreams: 1 Vanessa Emanuelle Pereira Santos.pdf: 1410254 bytes, checksum: c10ced66b2b2a50acd99e2b5cb8651e8 (MD5) Previous issue date: 2018-03-01 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The mangabeira is a native species of Brazil, widely distributed and that presents socioeconomic importance mainly in the Northeast and Center-West regions of the country. In Brazil, H. speciosa occurs in the Caatinga, in the Cerrado, in the Coastal Tracks and in the Restingas. Its fruits contain vitamin C and have the highest levels of protein and iron and its leaves have medicinal properties being used to control blood pressure and to treat diabetes. Due to the real estate expansion, use of native areas for cultivation of large crops and fragmentation of the forests, the species has suffered significant genetic erosion. Despite its importance and vulnerability genetic and genomic studies in H. speciosa and therefore projects with the purpose of knowing and studying the genomic and genetic characteristics of the species are important in order to contribute to the conservation and genetic improvement of the species. Plants generally contain high proportions of repetitive DNA, which are important elements of gene regulation and that appear along the evolution causing mutations that can change phenotypically the organism in question. In this sense, the objective of this work was to characterize the repetitive fraction of H. speciosa providing the first genomic study in the species which will contribute in future to techniques applied to genetic improvement involving genomic association. Mangrove individuals from the State of Pernambuco were collected and their DNA extracted and later fragmented to construct the new generation sequencing paired-end-reads (NGS) library, using the Illumina HiSeq2500 platform. Genome size was estimated using flow cytometry and the repetitive fraction was analyzed using the RepeatExplorer and TAREAN software. Three species genetically related to H. speciosa were used to compare the results and to evaluate if the amount of repetitive DNA is related to the genetic proximity of the species. The genome of H. speciosa has a small size (430 Mb) and a small amount of repetitive DNA (15%) which comes mainly from LTR / Gypsy elements and from rDNA sequences. The species does not present satellite DNA sequences and we suggest that the role of this element in the genome is assumed by LTR / Gypsy chromovirus sequences which have a large proportion in genomes, especially in centromeric regions. The information obtained in the present study provides initial information to the genomic studies in H. speciosa which will contribute to the genetic improvement of the mangaba. A mangabeira é uma espécie nativa do Brasil, amplamente distribuída no Brasil, e que apresenta importância socioeconômica, principalmente nas regiões Nordeste e Centro-Oeste do país. Hancornia speciosa ocorre na Caatinga, no Cerrado, nos Tabuleiros Costeiros e nas Restingas. Seus frutos contêm vitamina C e apresentam os maiores teores de proteína e ferro e suas folhas possuem propriedades medicinais sendo utilizadas no controle da pressão arterial e no tratamento contra diabetes. Devido à expansão imobiliária, utilização de áreas nativas para cultivo de grandes culturas e fragmentação das florestas, a espécie vem sofrendo significativa erosão genética. Apesar de sua importância e vulnerabilidade, estudos genômicos em H. speciosa são negligenciados, sendo assim, o conhecimento de suas características genômicas, os quais permitirão a aplicação de técnicas que contribuam com programas de conservação e melhoramento genético da espécie, são de suma importância. As plantas, geralmente, contêm elevadas proporções de DNA repetitivo, os quais são importantes elementos de regulação gênica e que surgem ao longo da evolução, ocasionando mutações que podem alterar fenotipicamente o organismo em questão. Neste sentido, o objetivo deste trabalho foi caracterizar a fração repetitiva de H. speciosa fornecendo o primeiro estudo genômico na espécie o qual contribuirá futuramente para técnicas aplicadas ao melhoramento genético envolvendo associação genômica. Indivíduos de mangaba, provenientes do Estado de Pernambuco foram coletados e tiveram seu DNA extraído e posteriormente fragmentado para construção da biblioteca paired-end-reads de sequenciamento de nova geração (NGS), usando a plataforma Illumina HiSeq2500. O tamanho do genoma foi estimado usando citometria de fluxo e a fração repetitiva foi analisada utilizando o software RepeatExplorer e TAREAN. Três espécies relacionadas geneticamente com H. speciosa foram utilizadas para comparar os resultados e avaliar se a quantidades de DNA repetitivo tem relação com a proximidade genética das espécies. O genoma de H. speciosa possui tamanho pequeno (430 Mb) e pouca quantidade de DNA repetitivo (15%) a qual é proveniente principalmente de elementos LTR/Gypsy e de sequências de DNAr. A espécie não apresenta sequências de DNA satélite e sugerimos que o papel deste elemento no genoma é assumido por sequências LTR/Gypsy chromovirus, os quais possuem ampla proporção em genomas, principalmente em regiões centroméricas. As informações obtidas no presente estudo fornecem informações iniciais aos estudos genômicos em H. speciosa os quais contribuirão para o melhoramento genético da mangaba.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior A obtenção de mudas de qualidade é fundamental para o sucesso da implantação de um projeto florestal, pois o crescimento e a sobrevivência das plantas estarão atrelados à sua qualidade. A nutrição mineral e a seleção de microrganismos simbiontes a serem adicionados ao substrato de cultivo são de grande importância e influenciam a qualidade de uma muda. Contudo, para grande parte das espécies arbóreas nativas do Cerrado, esse conhecimento ainda não foi obtido, podendo dificultar ou inviabilizar a produção das mudas. O presente estudo teve como objetivo avaliar o crescimento inicial e a qualidade de mudas de Hancornia speciosa (mangabeira) e Brosimum gaudichaudii (mama-cadela) em resposta a doses de P e inoculação de microrganismos simbiontes provenientes do solo próximo às plantas matrizes e inóculo de fungos micorrízicos arbusculares (FMA). Foram avaliados dois experimentos, sendo ambos conduzidos no delineamento inteiramente casualizado (DIC). O experimento um foi composto por cinco doses de P (0, 100, 200, 300 e 400 mg dm -3 ), com seis repetições, totalizando 30 unidades experimentais por espécie, sendo o substrato uma mistura do solo rizosférico de plantas matrizes (SRPM) das espécies em estudo + horizonte subsuperficial de um Latossolo Vermelho-Amarelo distrófico autoclavado (LVAd-Aut) + substrato comercial Tropstrato Florestal® autoclavado (1:1:1,v:v:v). O experimento dois foi composto por quatro formulações de substrato contendo SRPM, LVAd-Aut e substrato comercial autoclavados (SC-Aut), onde esses receberam ou não a adição de inóculo de FMA (Mix), composto pelas espécies Gigaspora decipiens, Rhizophagus clarus e Scutellospora heterogama. Os tratamentos deste experimento consistiram de: T1 (LVAd-Aut + SC-Aut), T2 (LVAd-Aut + SC-Aut + Mix), T3 (LVAd-Aut + SC-Aut + SRPM) e T4 (LVAd-Aut + SC-Aut + SRPM + Mix), com seis repetições, totalizando 24 unidades experimentais por espécie. Ao final de cada experimento, realizou-se a determinação da taxa de colonização micorrízica (CM), a abundância de esporos presentes no substrato (AE) e as medições da altura total da parte aérea (H), diâmetro do coleto (DC), volume do sistema radicular (VR), massa de matéria seca da parte aérea (MSPA), raízes (MSR) e total (MST); relações H/DC, H/MSPA, MSPA/MSR, Índice de Qualidade de Dickson (IQD) e, para o experimento dois, foi quantificada, também, a dependência micorrízica (DM). No experimento um, plantas de mangabeira sofreram redução da CM em resposta aos níveis de P testados e tiveram resposta linear positiva à aplicação de P ao analisar as relações H/MSPA e MSPA/MSR, enquanto a relação H/DC não sofreu efeito do P. As demais variáveis em mudas de H. speciosa foram influenciadas negativamente pela aplicação de P. Em relação ao experimento dois, as espécies em estudo tiveram DM variando de moderada a alta quando o SRPM foi adicionado ao substrato de cultivo. A adição do SRPM gerou incremento na H, DC, VR, MSPA, MSR, MST e aumento da qualidade de mudas de H. speciosa e B. gaudichaudii, em comparação às plantas do tratamento controle. Para a produção de mudas de qualidade de H. speciosa e B. gaudichaudii, ao usar o substrato utilizado neste ensaio, a adubação fosfatada é desnecessária, sendo recomendada a utilização de solo rizosférico da planta matriz e, ou inoculação com FMA para garantir a produção de mudas de mangabeira e mama-cadela. Obtaining seedlings of quality is essential to the successful implementation of a forestry project, because the growth and survival of plants will be tied to its quality. Mineral nutrition and selection of symbionts microorganisms to be added to the substrate of seedling production are of great importance and influence the quality of the seedlings. However, for most of the native tree species of Cerrado, this knowledge has not yet been obtained, and may hinder or derail the production of seedlings. The present study aimed to evaluate the initial growth and the quality of seedlings of Hancornia speciosa (mangabeira) and Brosimum gaudichaudii (mama-cadela) in response to P and inoculation doses of microorganisms symbionts from the soil around the plants and inoculum of arbuscular mycorrhizal fungi (AMF). Two experiments were evaluated, both being conducted in completely randomized design (CRD). The experiment was composed of five doses of P (0, 100, 200, 300 and 400 mg dm -3 ), with six repetitions, totalizing 30 experimental units per species, being the substrate a mixture of rhizospheric soil coming from the mother plant species (RSMP) + subsurface horizon of a Latossolo Vermelho-Amarelo distrófico (Oxisol) autoclaved (LVAd-Aut) + commercial Forest substrate autoclaved (SC-Aut) (1:1:1, v:v:v). The experiment two was composed of four substrate formulations containing RSMP, LVAd-Aut and SC-Aut, where these had or had not received the addition of AMF inoculum (Mix), composed by the species Gigaspora decipiens, Rhizophagus clarus and Scutellospora heterogama. The treatments evaluated in experiment two were T1 (LVAd-Aut + SC-Aut), T2 (LVAd-Aut + SC-Aut + Mix), T3 (LVAd-Aut + SC-Aut + RSMP) and T4 (LVAd-Aut + SC-Aut + RSMP + Mix), with six repetitions, totalizing 24 experimental units per species. At the end of each experiment, the determination of the rate of mycorrhizal colonization (CM), the abundance of spores present in the substrate (AE) and measurements of the total height of shot (H), stalk diameter (DC), volume of the root system (VR), mass of shoot dry matter (MSPA), mass of roots dry matter (MSR), and total dry matter (MST),beyond their relationships, and the Dickson’s quality index (IQD) and, for the experiment two, was quantified also the mycorrhizal dependence (DM). In one experiment, plants of mangabeira suffered reduction of CM in response to P levels tested and had positive linear response to P application to analyze relations H/MSPA and MSPA/MSR, while the ratio H/DC suffered no effect of P. The other variables in seedlings of H. speciosa were influenced negatively by applying P. compared to experiment two, the studied species had DM ranging from moderate to high when the RSMP was added to the substrate. The addition of the RSMP generated increase in H, DC, VR, MSPA, MSR, and MST, and increased the quality of seedlings of H. speciosa and B. gaudichaudii, as compared to the control treatment seedlings. For the production of quality seedlings of H. speciosa and B. gaudichaudii, when using the substrate used in this test, the phosphate fertilization it is recommended to use of rhizospheric soil of the mother plant and, or inoculation with AMF for guarantee the seedling production of mangabeira and mama- cadela.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The mangabeira (Hancornia speciosa Gomes) is a fruit species that is native to Brazil. In the State of Sergipe, the mangabeira has a social, cultural and economic importance. The fragmentation of the natural areas of the species has intensified and in this context, the Mangaba Genebank (BAG Mangaba) was implemented. The two specific research investigations of this work were carried out at the Embrapa Coastal Tablelands and at the University of Florida, USA, in order to evaluate the various aspects concerning the propagation and the genetic diversity of the species. In the first experiment, the propagation and the biometrics of the fruits and seeds of the BAG Mangaba accessions were evaluated. In the biometric studies, the weight and the dimensions (length, width and thickness) of fruits and seeds were evaluated, as well as the number of seeds per fruit. The propagation analyzes and the experimental design were completely randomized, with 4 replicates of 25 seeds, totaling 100 seeds per treatment. In this study, the seeds were sown in polyethylene bags that contained sand as a substrate. They were then submitted to the following determinations: emergence (E%), emergency speed indexes (ESI), seedling heights (cm), diameters (DIA) and the percentages of survival (S%), together with the fruit and seed biometrics (weight, length and width, together with the thickness of the fruits and seeds, as well as the number of seeds per fruit). The second study was conducted at the University of Florida, USA, with the objective of evaluating the genetic diversities of the accessions and the progenies of the mangaba fruit, by using microsatellite markers (SSR). A total of 96 samples were used, 9 matrices and 87 progenies. The genetic diversities were measured by using the following genetic parameters: the allele numbers (N), the allelic richness (R), the expected heterozygosity (He), the observed (Ho) genetic divergence indexes (Fst), the endogamy coefficients (f), Nei’s genetic identities (GI), the parental relationship between the progenies and the matrices, as well as the means of the clusters, conducted by Bayesian analyzes. In a third experiment, the researchers calculated the genetic diversities of 155 individuals from 10 natural populations of mangabeira in the State of Sergipe, by using ISSR markers. The genetic diversities were measured as a result of the following parameters: the polymorphism percentages, the coefficients of variation, Shannon’s indices (I), the expected heterozygosities (He), the similarity among the individuals, the UPGMA groupings, the principal coordinate analyzes (PCoA), as well as by using AMOVA. The accessions showed divergences regarding their biometric characteristics and there was a significant asymmetry between the progenies in their propagative variables, highlighting the progeny accessions from the States of Pará (ABP1, ABP2 and ABP4), Sergipe (PTP1, PTP2, TCP2 and TCP6) and Bahia (LGP1, LGP3, LGP4 and BIP2). For the molecular analyzes, it was verified that the SSR markers showed a moderate genetic variability between the progenies and the matrices, with the progenies from the accessions in the State of Pará (ABP1 and ABP2) being the most divergent. In the natural populations of Sergipe, when making the use of the ISSR markers, a high polymorphism rate (100%) and the formation of five large groups were observed. The mangabeira is a species that is still in the stage of domestication and studies that involve their propagation, their morphology and their genetic diversities are important for the development of strategies for the conservation and the selection of plants, with characteristics of interest in various breeding programs. A mangabeira (Hancornia speciosa Gomes) é uma espécie frutífera, nativa do Brasil, de ocorrência em restingas, cerrado e tabuleiros costeiros. No estado de Sergipe a mangabeira possui importância social, cultural e econômica. A fragmentação de áreas naturais de ocorrência da espécie tem se intensificado, e neste contexto, foi implantado o Banco Ativo de Germoplasma Mangaba (BAGMangaba). As pesquisas deste trabalho foram realizadas na Embrapa Tabuleiros Costeiros e na Universidade da Flórida, com o objetivo de avaliar aspectos sobre a propagação e a diversidade genética da espécie. No primeiro experimento avaliou-se a propagação e a biometria dos frutos e sementes de acessos do BAGMangaba. No estudo biométrico avaliou-se o peso e as dimensões (comprimento, largura e espessura) de frutos e sementes, além do número de sementes por fruto. Para as análises de propagação, utilizou-se o delineamento experimental inteiramente casualizado com quatro repetições de 25 sementes, totalizando 100 sementes por tratamento. Neste estudo, as sementes foram beneficiadas, logo após, semeadas em saco de polietileno, contendo areia como substrato, e em seguida submetidas aàs seguintes determinações: porcentagem de emergência (E%), índice de velocidade de emergência (IVE), altura de mudas (ALT), diâmetro do caule (DC), número de folhas (NF) e porcentagem de sobrevivência (S%). O segundo estudo foi realizado na Universidade da Flórida com o objetivo de avaliar a diversidade genética de acessos e progênies de mangaba, utilizando marcadores microssatélies (SSR). Foram utilizadas 96 amostras, sendo 9 matrizes e 87 progênies, e a diversidade genética foi mensurada por meio dos seguintes parâmetros genéticos: número de alelos (N), riqueza alélica (R), heterozigosidade esperada (He) e observada (Ho), índice de divergência genética (Fst), coeficiente de endogamia (f), identidade genética de Nei (GI), relação de parentesco entre progênies e matrizes, agrupamentos por meio da análise Bayesiana. No terceiro experimento estimou-se a diversidade genética de 155 indivíduos em 10 populações naturais de mangabeira no estado de Sergipe, utilizando marcadores ISSR. A diversidade genética foi mensurada pelos seguintes parâmetros: porcentagem de polimorfismo, coeficiente de variação, índice de Shannon (I), heterozigosidade esperada (He), similaridade entre indivíduos, agrupamento UPGMA, análise de coordenadas principais (ACoP) e AMOVA. Os acessos mostraram-se divergentes quanto às características biométricas, e houve significativa assimetria entre as progênies nas variáveis de propagação, destacandos-se as progênies dos acessos provenientes dos estados do Pará (ABP1, ABP2 e ABP4), Sergipe (PTP1, PTP2, TCP2 e TCP6) e Bahia (LGP1, LGP3, LGP4 e BIP2). Para as análises moleculares verificou-se que os marcadores SSR revelaram moderada variabilidade genética entre progênies, sendo aquelas progênies provenientes dos acessos do Pará (ABP1 e ABP2) as mais divergentes. Em populações naturais de Sergipe com o uso de marcadores ISSR verificou-se alta taxa de polimorfismo (100%) e a formação de cinco grandes grupos. A mangabeira é uma espécie ainda em fase de domesticação, e estudos que envolvam a propagação, morfologia e diversidade genética são importantes para o desenvolvimento de estratégias de conservação e seleção de plantas com características de interesse em programas de melhoramento genético. São Cristóvão, SE

Made available in DSpace on 2020-12-12T02:10:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-17 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background: Hancornia speciosa is usually used in Brazilian folk medicine to treat diabetes. The hypothesis of the present study is that this medicinal plant exerts beneficial effects on hyperglycemia, preventing diabetic complications. Therefore, the aim of this study was to evaluate the treatment effect of the aqueous extract of H. speciosa leaves on metabolic parameters of diabetic rats. Methods: The H. speciosa extract (400 mg/Kg) was administered to both nondiabetic and severely diabetic female Wistar rats by gavage. The Oral Glucose Tolerance Test was performed and the area under the curve (AUC) was estimated on day 17 of pregnancy. After 21 days of treatment, the animals were anesthetized and killed to obtain organ weights. Blood samples were collected for an analysis of serum biochemical parameters. Results: After treatment with the H. speciosa extract, the parameters of nondiabetic rats remained unchanged. In treated diabetic rats, glycemia, AUC, dyslipidemia parameters, and relative organ weights were decreased compared with nontreated diabetic rats. Severely diabetic rats showed decompensated hyperglycemia, polydipsia, hyperphagia and dyslipidemia. However, the aqueous extract of H. speciosa leaves decreased diabetes complications (indicating a lack of toxicity), reduced blood glucose levels, and exerced lipid-lowering effects. Conclusion: Based on or findings, the H. speciosa leaf extract may be a safe and promising candidate treatment for diabetes and other diseases. Laboratory of System Physiology and Reproductive Toxicology Institute of Biological and Health Sciences Federal University of Mato Grosso (UFMT), Av. Valdon Varjão 6390 Laboratory of Experimental Research on Gynecology and Obstetrics Botucatu Medical School Univ Estadual Paulista_Unesp, Distrito de Rubião Júnior s/n Laboratory of Experimental Research on Gynecology and Obstetrics Botucatu Medical School Univ Estadual Paulista_Unesp, Distrito de Rubião Júnior s/n

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The mangabeira its figure out among the mains native fruit tree explored by extractivism in Brasil. The objective evaluate the genetic divergence of landraces in orientation of crosses using multivariate techinics. The complete random blocks experimental design with four repetitions was used to evaluate twelve quantitative characteristics from twelve genotypes elite of mangabeiras concerning to divergence genetic using the software R (2012). Three groups genetically divergent were composed by biplot graphic and stored by UPGMA cluster analysis (Unweighted Pair-Group Method using Arithmetic Average / Weighted Clustering Method not using the Arithmetic Mean) showing genetic diversity and variability among 12 mangabeira accesses. Forty-four possible crosses are planned among genotypes of genetically dissimilar three groups and six among individuals in group III. Multivariate techniques were appropriate in the study of genetic divergence. Made available in DSpace on 2018-08-02T00:56:00Z (GMT). No. of bitstreams: 1 26665.pdf: 882569 bytes, checksum: cc24885f08b6d43970c2cbba4e526af8 (MD5) Previous issue date: 2018-08-01

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Made available in DSpace on 2018-12-26T23:39:44Z (GMT). No. of bitstreams: 1 CBRG2018p448.pdf: 232434 bytes, checksum: 8d28f12fa86a65418869f5f5fdae85e0 (MD5) Previous issue date: 2018-12-20 Edição especial dos Anais do 5º Congresso Brasileiro de Recursos Genéticos, Fortaleza, nov. 2018.

Hancornia speciosa Gomes is a Brazilian native tree. Its fruit, mangaba, has both social and economic importance and is collected by traditional people, mainly women. However, its natural habitats are being threatened by anthropic pressure. Our aim in this work was to characterize the population structure of H. speciosa along the Mangabeira trail at the Reserva do Caju (11°6?10.12? S, 37°11?4.59? W) located in Sergipe, Brazil. We sampled all mangabeira individuals in the trail and divided them into three classes of development (seedlings, juvenile and adult trees). In order to analyse their horizontal and vertical distribution, we measured the trees' diameter and height. The population structure of H. speciosa at the Mangabeira trail is mainly composed of seedling (46%) and juvenile (29%) individuals, which indicates a regeneration process. The diameter distribution of seedlings and adults was divided into six classes. The three lowest classes accounted for 94% of the total of trees. We also observed a typical ?inverted J? pattern, with a high number of young plants, which is common to tropical forests. In terms of their vertical distribution, the majority of the individuals were classified under the lower stratum, which corresponded to 47% of the total population featuring 1.01-m average height. The high coefficient of variation in the lower stratum may explain the heterogeneity of the vertical structure, formed by individuals with different strategies to capture sun light and different behaviour towards resources supply. Thus, it is not possible to state that this population is in balance. Made available in DSpace on 2018-10-04T00:47:57Z (GMT). No. of bitstreams: 1 4977.pdf: 529117 bytes, checksum: 1fb1d6c8a782e140a1abcce3e067cfcc (MD5) Previous issue date: 2018-10-03

Mangaba (Hancornia speciosa Gomes) is a native fruit to Brazil of great importance in its areas of occurrence. The aim of the present study was to evaluate the physiological behavior of mangaba seeds submitted to desiccation. The survey was conducted in the Laboratory of Seeds Analyses of Embrapa Coastal Tablelands, Aracaju, SE, Brazil. The experimental design was completely randomized with five replicates of 25 seeds each. Seeds were submitted to treatments consisting of different drying times: 0 (control treatment), 24, 48 and 72 h. Seeds were placed in an oven with air circulation and temperature of 30oC. The water content of seeds was determined and the germination test was used to evaluate the germination percentage, speed germination index and length and dry weight of seedlings. The seed water content decreased as a function of the drying periods, reaching 13% when seeds were submitted to 72 h of drying. Seeds submitted to drying periods up to 33 h showed decreased germination percentage, dry weight and length of seedlings; the germination rate index and plant growth were also affected after 34 h of drying. Drying periods longer than 34 h impair the physiological quality of mangaba seeds, affecting germination and vigor. Key words: Hancornia speciosa Gomes, desiccation, water content.

The Brazilian cerrado has several fruit species with great potential for agro-industrial use including the production of milk drinks. Thus, the objective of this study was to evaluate the sensory profile and purchase intent of fermented milk drinks added in increasing levels of fruits from the Brazilian cerrado. Four formulations of milk drinks were processed with concentrations of 4, 8, 12 and 16% of Araca (Psidium cattleianum), Araticum (Annona crassiflora Mart), Mangaba (Hancornia speciosa Gomes), Passion fruit (Passiflora edulis f. flavicarpa) and Pequi pulps (Caryocar brasiliense Camb.). The sensory profile of products was characterized by affective test evaluating acceptance using the hedonic scale and purchase intent. Fruits used in flavoring, especially Pequi, are very appreciated by the local population. As demonstrated in acceptability tests, it is believed that this factor contributed to the high acceptance of Pequi. All milk drinks showed positive purchase intent value. Key words: Fermented milk, acceptance test, purchase intent.

Ethnopharmacological relevance The leaves of Hancornia speciosa Gomes are traditionally used to treat diabetes in Brazil. The aim of the study is to evaluate the potential anti-diabetic effect of Hancornia speciosa extract and derived fractions. Materials and Methods The ethanolic extract from Hancornia speciosa leaves and chromatographic fractions thereof were evaluated on α-glucosidase assay, on hyperglycemic effect and glucose uptake. The chemical composition of the extract and its most active fraction was investigated by ESI–LC–MS. Results The ethanolic extract and derived fractions inhibited α-glucosidase in vitro. However, only the crude extract and the dichloromethane fraction inhibited the hyperglycemic effect induced by starch or glucose. Both the extract and dichloromethane fraction were also able to increase glucose uptake in adipocytes. Bornesitol, quinic acid, and chorogenic acid were identified in the extract, along with flavonoid glycosides, whereas the dichloromethane fraction is majorly composed by esters of lupeol and/or α/β-amirin. Conclusions Hancornia speciosa has a potential anti-diabetic effect through a mechanism dependent on inhibition of α-glucosidase and increase on glucose uptake. These results give support to the use on traditional medicine of this medicinal plant.

Considering the wide distribution of Hancornia speciosa Gomes in the Brazilian savanna, environment with irregular distribution of rainfall, it is believed that their seedlings can be cultivated for a few days under water restriction, information that can stimulate the use of this species in restoration or planting for sustainable exploration programs. The present study evaluated the gas exchange and photosynthetic activities of H. speciosa seedlings and examined their tolerance to water deficit and their metabolic recovery after rehydration. We evaluated the photosynthetic rate, internal carbon concentration, transpiration rate, stomatal conductance, water-use efficiency, photosystem II quantum efficiency (Fv/Fm), instantaneous carboxylation efficiency, chlorophyll index, and recuperation potential of H. speciosa seedlings after rehydration. Twelve month-old seedlings were used and maintained at 70% of their soil water retention capacity. Data was collected 7, 10, 12, 14, 16, 18, 20, 23, 31, 33, 35, 37, 42, 44, 46, and 48 days after suspending irrigation; irrigation was reinitiated when the photosynthetic rate approached zero. Water deficit conditions reduced all of the parameters evaluated except the chlorophyll index; stressed seedlings required 42 days for the photosynthetic rate to reach zero, but photosynthetic equilibrium was reestablished just five days after rehydration. This temporary water deficiency did not cause any permanent deleterious effects on the photosynthetic apparatus of the seedlings.

Brazil is the third largest fruit producer in the world, but many native fruits remain underexploited, as is the case for mangabeira (Hancornia speciosa Gomes). Little is known about general aspects of this species in its naturally occurring environment. Thus, this study aimed to characterize plant and fruit morphologies from native mangabeira selections in the Savannah-Restinga vegetation transition zone. The experiment was carried out in Patizal, Recanto and Recurso villages located in the Morros municipality, Maranhão State, dominated by extensive Savannah-Restinga vegetation transition zone with naturally occurring mangabeiras. Forty five mangabeira trees were randomly chosen from native selections in the three villages. Data collected were plant height, stem height, stem diameter, canopy volume, number of main and secondary branches and number of fruits. Twenty fruits were collected per plant to determine the mass, length and diameter, pulp and skin mass, pulp yield, seed number and seed mass. There was a high degree of variability for all assessed variables except for the pulp yield (average = 86.69%, minimum = 76.75% and maximum = 91.11%; CV= 3.51%). Significant and positive high magnitude correlations were noted among the variables of fruit mass and pulp mass plus skin with seed number (r=0,01; ?=0,78 e ?=0,74, respectively). RESUMO O Brasil é o terceiro maior produtor de frutas do mundo, porém muitas fruteiras nativas continuam subexploradas, como ocorre com a mangabeira (Hancornia speciosa Gomes). Pouco se conhece sobre os aspectos gerais da espécie em seus ambientes de ocorrência natural. Desta forma, objetivou-se caracterizar a morfologia da planta e do fruto de mangabeiras silvestres selecionadas em áreas de ocorrência natural da espécie, em vegetação de transição entre o Cerrado e a Restinga. O experimento foi realizado no município de Morros-Maranhão, nos povoados Patizal, Recanto e Recurso, caracterizados por extensas áreas de vegetação de transição: Cerrado e Restinga, com ocorrência natural de mangabeira. Nos três povoados, selecionaram-se 45 mangabeiras-matrizes, das quais se mensuraram a altura total, altura do fuste, diâmetro do caule, volume de copa, número de ramos principais e secundários, e número de frutos. Coletaram-se 20 frutos por matriz e determinaram-se a massa, comprimento e diâmetro dos frutos, massa da polpa mais casca, rendimento de polpa, número e massa de sementes. Houve elevado grau de variabilidade para todas as variáveis estudadas, exceto para o rendimento de polpa (média = 86,69%, mínimo = 76,75% e máximo = 91,11%; CV = 3,51%). Há correlação positiva significativa de alta magnitude entre as variáveis massa do fruto e massa da polpa mais casca com o número de sementes (r=0,01; ?=0,78 e ?=0,74, respectivamente).

Submitted by Alison Souza (alisonsouza@ufgd.edu.br) on 2019-04-08T21:06:16Z No. of bitstreams: 1 UilsonPereiradosSantos.pdf: 6822485 bytes, checksum: 26e883f73259b1582d031250bedab32f (MD5) Made available in DSpace on 2019-04-08T21:06:16Z (GMT). No. of bitstreams: 1 UilsonPereiradosSantos.pdf: 6822485 bytes, checksum: 26e883f73259b1582d031250bedab32f (MD5) Previous issue date: 2017-07-29 Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT) Hancornia speciosa Gomes é uma árvore frutífera, popularmente conhecida como mangabeira e amplamente distribuída pelo Brasil. As folhas desta planta são utilizadas na medicina popular para diferentes aplicações medicinais. Desta forma, o objetivo deste estudo foi realizar a caracterização físico-química, identificar os antioxidantes lipofílicos, ácidos graxos e compostos fenólicos, determinar a qualidade e segurança microbiológica das folhas de Hancornia speciosa Gomes, bem como, avaliar as atividades antioxidante, antimicrobiana, citotóxica, antimutagênica e de inibição de enzimas relacionadas com doenças neurodegenerativas, inflamação, obesidade e diabetes do extrato etanólico das folhas de H. speciosa (EEHS). Análises químicas foram realizadas por colorimetria, cromatografia gás-líquido com detecção por ionização de chama (GC-FID) e cromatografia líquida acoplada a espectrometria de massas (LC/MS). Foram determinados o número total de colônias de mesófilos aeróbios, bolores e leveduras. A quantificação dos coliformes totais e Escherichia coli foi realizada com o kit SimPlates, e esporos de Clostridium sulfito-redutor, utilizando-se o método SulfiteAgar de polimixina-sufadiazina. A pesquisa de Salmonella spp. foi efetuada pelo 1-2 Test. Dentre as atividades biológicas do EEHS avaliadas, a antimicrobiana foi determinada pelas concentração inibitória mínima, bactericida e fungicida mínima e por zona de inibição do crescimento. Células leucêmicas Kasumi-1 foram utilizadas para avaliar a ação citotóxica e os mecanismos envolvidos na morte celular. A atividade antioxidante foi investigada pelo método de inibição da hemólise oxidativa induzida por 2,2`- azobis (2-amidinopropano) em eritrócitos humanos. A atividade antimutagênica foi determinada pelo teste de Ames e as atividades de inibição das enzimas acetilcolinesterase, butirilcolinesterase, tirosinase, hialuronidase, lipase, α- amilase e α-glicosidase foram avaliadas e comparadas a controles comerciais. Em conjunto, os resultados garantem a qualidade, segurança microbiológica e demonstram presença de carotenoides, ácidos graxos poli-insaturados e compostos fenólicos nas folhas de H. speciosa, bem como as atividades antioxidante, antimicrobiana, citotóxica, antimutagênica, antiobesidade e anti-hiperglicemiante do EEHS. Hancornia speciosa Gomes is a fruit tree, popularly known as mangabeira and widely distributed throughout Brazil. The leaves of this plant are used in folk medicine for different medicinal applications. Thus, the objective of this study was to perform the physicochemical characterization, to identify the lipophilic antioxidants, fatty acids and phenolic compounds, to determine the microbiological quality and safety of the leaves of Hancornia speciosa Gomes, as well as to evaluate the antioxidant, antimicrobial, cytotoxic, antimutagenic activities and inhibition of enzymes related to neurodegenerative diseases, inflammation, obesity and diabetes of the ethanolic extract of the H. speciosa (EEHS) leaves. Chemical analyzes were performed by colorimetry, gas-liquid chromatography with flame ionization detection (GC-FID) and liquid chromatography coupled to mass spectrometry (LC / MS). The total number of colonies of aerobic mesophiles, molds and yeasts were determined. The quantification of total coliforms and Escherichia coli was carried out using the SimPlates kit, and sulfide-reducing Clostridium spores, using the Sulfite-Agar method of polymyxin-sufadiazine. The Salmonella spp. was performed by the 1-2 Test. Among the biological activities of the EEHS evaluated, the antimicrobial was determined by minimum inhibitory concentration, minimal bactericide and fungicide and by zone of inhibition of growth. Kasumi-1 leukemic cells were used to evaluate the cytotoxic action and mechanisms involved in cell death. The antioxidant activity was investigated by the method of inhibiting oxidative hemolysis induced by 2,2'- azobis (2-amidinopropane) in human erythrocytes. The antimutagenic activity was determined by the Ames test and the activities of the inhibition of the enzymes acetylcholinesterase, butyrylcholinesterase, tyrosinase, hyaluronidase, lipase, α- amylase and α-glycosidase were evaluated and compared to commercial controls. Together, the results guarantee the quality, microbiological safety and demonstrate the presence of carotenoids, polyunsaturated fatty acids and phenolic compounds in the leaves of H. speciosa, as well as antioxidant, antimicrobial, cytotoxic, antimutagenic, antiobesity and antihyperglycaemic activities of the EEHS.

Submitted by Mario BC (mario@bc.ufrpe.br) on 2017-12-18T13:50:32Z No. of bitstreams: 1 Ana Kelly dos Santos Maia.pdf: 2230603 bytes, checksum: 2977bc5e48caa440b9f32ae7437e4195 (MD5) Made available in DSpace on 2017-12-18T13:50:33Z (GMT). No. of bitstreams: 1 Ana Kelly dos Santos Maia.pdf: 2230603 bytes, checksum: 2977bc5e48caa440b9f32ae7437e4195 (MD5) Previous issue date: 2017-07-21 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The mangabeira (Hancornia speciosa Gomes) is a native fruit tree of Brazil, widely distributed in the cerrado and coastal boards. It produces tasty, nutritious and widely used fruits, with great potential for economic exploration. However, the natural populations of H. speciosa are undergoing an intense process of genetic erosion as a result mainly of expansion of agricultural frontier and cities, without study on diversity and genetic structure of populations. The lack of such information is limited to the adoption of efficient collect and conservation of genetic resources. The objective of this work was to analyze the diversity and genetic structure, as well as to infer the phylogeographic patterns of the natural populations of H. speciosa on Northeast Coastal Boards of Brazil. Microsatellite or SSR (six loci) and cpDNA (trnH-psbA region) markers were used to estimate the genetic diversity indexes and to get phylogeographic inferences of four natural populations of H. speciosa (96 individuals) sampled in the States of Maranhão, Paraíba, Pernambuco and Sergipe. The species showed moderate genetic structuring (FST = 0.095) with greater genetic diversity within populations (83%) than among populations (17%). The FST values for the pair of populations showed the smallest genetic differentiation between Paraíba x Pernambuco (0.015) and a larger between Maranhão and Pernambuco (0.142). It was observed that the Maranhão population had larger differentiation among four one. In general, for the populations studied, the fixation index was zero or negative (-0.082). A cpDNA analysis revealed six haplotypes and the Sergipe population presents a greater diversity of haplotypes, showing a haplotype in common with the States of Paraíba and Pernambuco. The population of Maranhão presents haplotypes exclusive. A spatial molecular variance analysis revealed two groups with high genetic structure (FST = 0.898 and FCT = 0.899), with 89.92% of the variation between the groups. This information on diversity and genetic structure are important subsidies for optimizing ex-situ and in-situ conservation of the genetic resources of mangabeira on studied region and for search of pre-breeding research of this species. A mangabeira (Hancornia speciosa Gomes) é uma frutífera nativa do Brasil, amplamente distribuída no cerrado e nos tabuleiros costeiros. Produz frutos saborosos, nutritivos e de ampla utilização, com grande potencial para exploração econômica. Entretanto, as populações naturais de H. speciosa estão sofrendo intenso processo de erosão genética devido à especulação imobiliária e a expansão da fronteira agrícola, sem que tenha sido realizado estudo sobre a diversidade e estrutura genética. A carência de tais informações limita a adoção de estratégias eficientes de coleta e de conservação dos recursos genéticos. O objetivo deste trabalho foi analisar a diversidade e estrutura genética, bem como inferir os padrões filogeográficos das populações naturais de H. speciosa nos Tabuleiros Costeiros do Nordeste do Brasil. Marcadores microssatélites ou SSR (seis loci) e cpDNA (região trnH-psbA) foram utilizados com sucesso para estimar os índices de diversidade genética e inferências filogeofráficas de quatro populações naturais de H. speciosa (96 indivíduos) nos Estados do Maranhão, Paraíba, Pernambuco e Sergipe. A espécie mostrou moderada estruturação genética (FST=0,095) com maior variabilidade genética dentro de populações (83%) do que entre populações (17%). Os valores de FST para as populações combinadas aos pares mostrou que a menor diferenciação genética foi entre Paraíba x Pernambuco (0,015), e a maior diferenciação entre Maranhão x Pernambuco (0,142). Observou-se que a população Maranhão foi a que mais divergiu das demais. No geral, para as populações estudadas o índice de fixação foi nulo ou negativo (-0,082). A análise do cpDNA revelou seis haplótipos sendo que a população de Sergipe apresentou maior diversidade de haplótipos e um haplótipo em comum com os Estados da Paraíba e Pernambuco. A população do Maranhão apresentou haplótipos exclusivos. A análise de variância molecular espacial revelou dois grupos com elevada estruturação genética (FST = 0,898 e FCT = 0,899), com 89,92% da variação entre os grupos. Tais informações de diversidade e estrutura genética constituem importantes subsídios para aperfeiçoar a conservação ex situ e in situ dos recursos genéticos da mangabeira na região estudada e para pesquisas de prémelhoramento genético dessa espécie.

Mangabeira fruits are collected from native populations under anthropic pressure. It is therefore essential to obtain morphogenetic information to genetically improve populations, thereby preventing genetic erosion. This research was carried out in the state Amapá and aimed to verify the morphological dissimilarity between two populations, one composed of 36 native progenies and one of eight progenies from Paraiba, outlined in lattices with two replications and six plants per part. The evaluated characteristics were: agronomic value (VA), treetop circumference (CCP), plant height (ALP), estimated number of fruits per plant (NEF), fruit color (COF), average weight of 10 fruits (PMF), fruit diameter (DF), average pulp weight of ten fruits (PMP), average length of 10 fruits (CMF), average number of seeds of 10 fruits (NMS). Our results indicate that the phenotype was mainly determined by responses to environmental changes; in the correlations, inherent fruit characteristics (weight, size, and color) did not affect the VA, while biometric characters influenced VA values. Higher plants presented larger fruits and a larger diameter, bigger quantities, there is also apparent contrast, with a larger amount of smaller size; the populations are dissimilar, indicating the possibility of obtaining of diverging superior segregating. RESUMO As frutas de mangabeiras são coletadas extrativamente em populações nativas sob grande pressão antrópica, devendo-se obter informações morfogenéticas para ações com reflexos no melhoramento genético que possam evitar a sua erosão genética. Esta pesquisa realizada no Amapá, teve como objetivo verificar a dissimilaridade morfológica entre duas populações, uma composta por 36 progênies nativas e outra com 8 da Paraíba, delineados em látices, com duas repetições e seis plantas por parcela. As características avaliadas foram: valor agronômico (VA); circunferência da copa da planta (CCP); altura da planta (ALP); número estimado de frutos na planta (NEF); cor do fruto (COF); peso médio de dez frutos (PMF); diâmetro do fruto (DF); peso médio da polpa de dez frutos (PMP); comprimento médio de dez frutos (CMF); número médio de sementes de dez frutos (NMS). As principais conclusões obtidas indicaram que o fenótipo foi devido principalmente as respostas às variações ambientais; nas correlações, características inerentes aos frutos (peso, tamanho e cor) não influenciaram o VA, enquanto que os caracteres biométricos influenciaram, plantas mais altas apresentaram frutos maiores e as com maior diâmetro maior quantidade, há também aparente compensação, com maior quantidade de frutos, estes são menores; as populações são dissimilares, indicando possibilidade da obtenção de segregantes divergentes superiores.

The fruit of the caatinga has aroused growing interest on the part of the food industry because of its potential health benefits. Exotic fruits such as seriguela (Spondias purpurea), umbu-cajá (Spondias spp.) and mangaba (Hancornia speciosa) are important sources of bioactive compounds, such as phenolics. However, the high perishability of these fruits is a factor that hampers their in natura supply, which leads to their processing, which in turn has a direct impact on the content of bioactive present due to the process of structural modification through reactions that may interfere in the bioaccessibility of bioactive compounds, causing nutritional losses. For these reasons, the objective of this work was to determine the phenolic profile of mango, seriguela and umbu-cajá fruits and their respective pulps, evaluating the antioxidant capacity and checking the behavior of phenolic compounds when exposed to simulated gastrointestinal digestion and post-digestion bioaccessibility . In this study, phenolic compounds were identified and quantified by High Performance Liquid Chromatography (HPLC) on fruits of seriguela (Spondias purpurea), umbu-cajá (Spondias spp.) and mangaba (Hancornia speciosa), and their respective pulps and Fractions from gastrointestinal digestion. The antioxidant activity of the bioacessible fraction after in vitro simulated gastrointestinal digestion was quantified by the DPPH and FRAP assays. From the determined phenolic profile of fruits compared to their respective frozen pulps, a reduction in the phenolic compounds content of fruits of the genus Spondias spp. In the mangaba fruit there was an increase in some increased free phenolic contents, such as 3,4-dihydroxybenzoic acid (+ 50%), and p-coumaric acid (+ 50%). In the fruit pulp the reduction of the phenolic compounds was observed in front of the antioxidant tests, in which the free radical scavenging capacity reduced after the fruit processing. From the simulated gastrointestinal digestion it was observed that fruits of the genus Spondias have a high fraction of their bioaccessible phenolic compounds after digestion, with a bioaccessibility of 73.92% (gallic acid, umbu-cajá pulp), unlike mangaba 5 , 60% (ferulic acid). The results obtained in this work demonstrate that the pulp consumption of these fruits, especially seriguela and umbu-cajá can contribute significantly to the ingestion of antioxidant compounds, since they have high concentration of bioaccessible phenolic compounds. Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES As frutas da caatinga tem despertado crescente interesse por parte da indústria de alimentos devido aos seus potenciais benefícios à saúde. Frutas exóticas como a seriguela (Spondias purpurea), umbu-cajá (Spondias spp.) e mangaba (Hancornia speciosa) são importantes fontes de compostos bioativos, como os fenólicos. Entretanto, a alta perecibilidade dessas frutas é um fator que dificulta sua oferta in natura, o que leva ao processamento das mesmas, que por vez tem impacto direto no conteúdo de bioativos presentes devido ao processo de modificação estrutural mediante reações que podem interferir na bioacessibilidade dos compostos bioativos, ocasionando perdas nutricionais. Por estas razões o presente trabalho objetivou determinar o perfil fenólico das frutas de mangaba, seriguela e umbu-cajá e de suas respectivas polpas, avaliando a capacidade antioxidante e verificando o comportamento dos compostos fenólicos quando expostos a digestão gastrointestinal simulada e sua bioacessibilidade pós-digestão. Neste estudo, os compostos fenólicos foram identificados e quantificados por Cromatografia Liquida de Alta Eficiência (CLAE) nas frutas de seriguela (Spondias purpurea), umbu-cajá (Spondias spp.) e mangaba (Hancornia speciosa), e nas suas respectivas polpas e nas frações oriundas da digestão gastrointestinal. A aatividade antioxidante da fração bioacessível após a digestão gastrointestinal simulada in vitro foi quantificada através dos ensaios DPPH e FRAP. A partir do perfil fenólico determinado das frutas em comparação com a suas respectivas polpas congeladas, pôde-se identificar uma redução no conteúdo de compostos fenólicos das frutas do gênero Spondias spp. Na fruta mangaba houve aumento de alguns teores de fenólicos livres aumentados, como ácido 3,4-dihidroxibenzoico (+50%), e ácido p-cumarico (+50%). Na polpa em relação a fruta a redução dos compostos fenólicos foi observada frente aos ensaios antioxidantes, em que a capacidade sequestradora de radicais livres reduziu após o processamento das frutas. A partir da digestão gastrointestinal simulada observou-se que as frutas do gênero Spondias possuem uma elevada fração de seus compostos fenólicos bioacessíveis após a digestão, com uma bioacessibilidade de 73,92% (ácido gálico, polpa umbu-cajá), diferentemente da mangaba 5,60% (ácido ferúlico). Os resultados obtidos neste trabalho demonstram que o consumo da polpa destas frutas, especialmente seriguela e umbu-cajá podem contribuir significativamente para a ingestão de compostos antioxidantes, ja que os mesmos possuem alta concentração de compostos fenólicos bioacessiveis.

O látex de diversas plantas apresenta potencial biotecnológico para desenvolvimento de novos fármacos. Dentre as lactíferas, a mais conhecida e explorada comercialmente é a seringueira, a qual possui látex com propriedade angiogênica. A fração F1 do látex de seringueira originou o Regederm® , creme-gel utilizado no tratamento de lesões teciduais. Contudo, existem relatos sobre alergias a produtos a base do látex de seringueira. Por esse motivo, tem-se buscado outras espécies lactíferas produtoras de látex com menor potencial alergênico. A mangabeira (Hancornia speciosa Gomes), planta nativa do cerrado e pouco explorada comercialmente, pode ser uma alternativa, uma vez que essa planta produz um látex com menor quantidade de proteínas, biocompatível e com propriedades angiogênicas. O objetivo desse trabalho foi fracionar o látex de mangabeira e avaliar o potencial angiogênico de suas frações; visando identificar qual dessas frações tem potencial para ser utilizado como biomaterial para aplicações médicas, e também determinar quais os principais metabólitos secundários que constituem a fração angiogênica. Para avaliar o potencial angiogênico, foi realizado o ensaio utilizando membrana corioalantóide (MCA) de ovos de galinha fertilizados. Os dados obtidos a partir desta técnica foram complementados pela análise molecular da expressão de genes relacionados à remodelamento tecidual e por prospecção fitoquímica da fração responsável pela maior atividade angiogênica do látex de mangabeira. Os resultados morfométricos obtidos pelo ensaio da MCA demonstraram um aumento significativo da rede vascular nas membranas tratadas com as frações soro e F1 do látex de mangabeira, quando comparadas ao controle neutro e controle inibidor. Além da avaliação morfológica das MCAs, foram realizadas também análises histológicas as quais identificaram maior intensidade de inflamação, células do conjuntivo e angiogênese nas MCAs submetidas às frações soro e F1 do látex de mangabeira, confirmando os dados morfométricos e sugerindo a atividade regenerativa do látex de mangabeira. As análises moleculares mostraram uma expressão aumentada do gene da metaloprotease 2, associado a angiogênese, nas MCAs tratadas com a fração F1 do látex de mangabeira. Assim, de forma geral, os dados obtidos no presente estudo revelaram que a fração soro do látex de mangabeira representa um importante agente angiogênico e que este biomaterial possui potencial terapêutico para ser explorado pelas ciências médicas. A prospecção química da fração soro, responsável pela maior atividade angiogênica, revelou a presença de flavonóides e taninos. Dados da literatura destacam a importância farmacológica desses compostos por serem substâncias que possuem atividade antioxidante, antimicrobiana e de forte potencial angiogênico, sendo capazes de atuar na aceleração do processo de regeneração tecidual. A utilização do soro do látex de mangabeira como um biomaterial em aplicações médicas, representa uma área bastante promissora, em especial, devido a sua capacidade de estimulação natural da angiogênese e por apresentar biocompatibilidade com sistemas vivos. Esses resultados agregam valor econômico a H. speciosa, a qual até o presente momento, vem sendo explorada comercialmente apenas pela utilização de seus frutos pela indústria alimentícia. The latex of several plants presents biotechnological potential for the development of new drugs. Among the lactiferous, the best known and commercially explored is the rubber tree, which has latex with angiogenic properties. The F1 fraction of natural rubber latex originated Regederm®, cream-gel used in the treatment of tissue lesions. However, there are reports of allergies to products based on natural rubber latex. For this reason, researchers have been looking for other latex producing lactiferous species with less allergenic potential. The mangabeira (Hancornia speciosa Gomes), a plant native to the Cerrado and poorly explored commercially, may be an alternative, since this plant produces a latex with less amount of proteins, biocompatible and with angiogenic properties. The objective of this work was to fractionate the mangabeira latex and to evaluate the angiogenic potential of its fractions; aiming to identify which of these fractions has the potential to be used as biomaterial for medical applications, and also to determine the main secondary metabolites that constitute the angiogenic fraction. To evaluate the angiogenic potential, the chorioallantoic membrane (CAM) assay was performed on fertilized chicken eggs. The data obtained from this technique were complemented by quantitative real-time PCR of genes related to tissue remodeling and by phytochemical prospection of the fraction responsible for the highest angiogenic activity of mangabeira latex. The morphometric results, obtained by the CAM assay, showed a significant increase of the vascular network in the membranes treated with the serum and F1 fractions of the mangabeira latex when compared to the neutral control and control inhibitor. In addition to the morphological evaluation of the CAMs, histological analysis were also performed, which identified a higher intensity of inflammation, conjunctival cells and angiogenesis in the CAMs submitted to the serum and F1 fractions of the mangabeira latex, confirming the morphometric data and suggesting the regenerative activity of mangabeira latex. Molecular analysis showed increased expression of the metalloprotease 2 gene, associated with angiogenesis, in CAMs treated with F1 fraction of mangabeira latex. Thus, in general, the data obtained in the present study shows that the serum fraction of mangabeira latex represents an important angiogenic agent and that this biomaterial has therapeutic potential to be explored by the medical sciences. The chemical prospection of the serum fraction, responsible for the highest angiogenic activity, showed the presence of flavonoids and tannins. Literature data highlight the pharmacological importance of these compounds to be substances that have antioxidant, antimicrobial and strong angiogenic potential, being able to act in the acceleration of the tissue regeneration process. The use of the mangabeira latex serum as a biomaterial in medical applications represents a very promising area, in particular due to its ability to naturally stimulate angiogenesis and to present biocompatibility with living systems. These results add economic value to H. speciosa, which until the present moment has been commercially explored only by the use of its fruits by the food industry. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Trees native to Brazil, mangaba (Hancornia speciosa Gomes) is a species mainly extractive, occurrence in various regions of the country. The Northeast holds of 99% of all production, being the Sergipe state the largest producer. The potential for the use of the pulp is quite varied, is used for fresh consumption and industrialization of various foods and drinks. The species has been threatened with extinction by several factors that are contributing to reduction of their naturally occurring areas. To enlarge the database for the use and knowledge of the variability of this species, as well as support to its domestication, it is necessary to carry out studies and characterization of genetic diversity. The present work was developed with the purpose to display and evaluate morphological descriptors, physico-chemical and molecular in germplasm of mangaba. The genebank of mangaba (BGmangaba) of Embrapa Coastal Tablelands was implemented in 2006 and has 213 individuals, representing 22 accessions. For morphological and physico-chemical characterization, 21 descriptors were used on 54 plants, from 10 access on fruit-bearing stage. There was wide variation and significance among and with access, without direct relationship with its origins. Fourteen descriptors were considered of significant importance for studies of characterization of mangaba, without loss of information on characterization. The accesses that presented the most significant values were AB, BI, CA, LG, TC, with greater development when compared to the others, the accesses TC, AB, AD, stand out as attractive, due to their physicochemical characteristics and the accesses BI and TC with fruits of larger masses, characteristic of interest for the in natura consumption and agroindustrial processing. All accesses of the BAG were used for molecular characterization and genetic structure, using nine microsatellite markers (SSR). 100% of polymorphism was observed with the use of SSR. 147 alleles were identified, with an average of 16 alleles for loco. Reliability was verified with stress values (0.042) and correlation (0.988). The alleles showed high frequency of heterozygosity (Ho>Ho). Fst values (0.22) and f (0.07) indicated moderate population structure, being presented greater diversity within the traffic. Bayesian analysis indicated a group with k=2, confirmed with the UPGMA. Were formed two distinct groups, grouped according to similarity. The pairs of individuals PM5 and GX2; CN1 and CN9; G18 and PA1; JA14 and JA15; OI8 and OI9, all belonging to the G2 were those closest genetically. The combination of the keywords used in this study, favors the identification of different individuals and with features of interest. The germplasm evaluated has diversity among and within access.The results will collaborate in the conservation of this material strategies and future breeding programs. The proposed descriptors will be used by FAO/Biodiversity. Frutífera nativa do Brasil, a mangabeira (Hancornia speciosa Gomes) é uma espécie de cultivo predominantemente extrativista, que ocorre em várias regiões do país. A região Nordeste detém 99% de toda a produção, e o estado de Sergipe é o maior produtor. O potencial para o aproveitamento da polpa é bastante variado, é utilizada para o consumo in natura e industrialização de diversos alimentos e bebidas. Para ampliar a base de dados e conhecimento da variabilidade desta espécie, bem como dar suporte à sua domesticação, é necessário que se realizem estudos de caracterização e de diversidade genética. O presente trabalho foi desenvolvido com a finalidade de indicar e avaliar descritores morfológicos, físico-químicos e moleculares em germoplasma de mangaba. O Banco de Germoplasma de Mangaba (BGMangaba) da Embrapa Tabuleiros Costeiros foi implantado em 2006 e possui 213 indivíduos, que representam 22 acessos. Para a caracterização morfológica e físico-química, 21 descritores foram utilizados em 54 plantas, oriundas de 10 acessos em fase de frutificação. Observou-se ampla variação e significância entre e dentro dos acessos, sem relação direta com a sua origem. Quatorze descritores foram considerados de significativa importância para estudos de caracterização de mangabeira, sem perda de informações na caracterização. Os acessos que apresentaram valores mais significativos foram AB, BI, CA, LG TC com maior desenvolvimento quando comparado aos demais, os acessos TC, AB, AD, se destacam como atrativos, por suas características físico-químicas, e os acessos BI e TC com frutos de maiores massas, característica de interesse para o consumo in natura e processamento agroindustrial. Todos os acessos do BGMangaba foram utilizados para a caracterização molecular e estrutura genética, utilizando nove marcadores microssatélites (SSR). Observou-se 100% de polimorfismo com o uso dos SSR. Foram identificados 147 alelos, com média de 16 alelos por loco. A confiabilidade foi verificada com valores de estresse (0,042) e de correlação (0,988). Os alelos apresentaram alta frequência de heterozigosidade (He>Ho). Os valores de Fst (0,22) e de f (0,07) indicaram moderada estrutura populacional, sendo apresentada maior diversidade dentro dos acessos. A análise Bayesiana indicou um agrupamento com k=2, confirmado com o UPGMA. Foram formados dois grupos distintos, agrupados de acordo com a similaridade. Os pares de indivíduos PM5 e GX2; CN1 e CN9; G18 e PA1; JA14 e JA15; OI8 e OI9, todos pertencentes ao G2 foram os mais próximos geneticamente. A combinação dos descritores utilizados nesse estudo, favorece a identificação de indivíduos mais divergentes e com características de interesse. A diversidade entre e dentro dos acessos foi confirmada com as análises moleculares. Os resultados irão colaborar nas estratégias de conservação desse material e futuros programas de melhoramento. A proposta de descritores será utilizada pela FAO/Biodiversity. São Cristóvão, SE

O presente artigo consiste de um levantamento histórico sobre a mangabeira em Pernambuco e teve o objetivo de resgatar a importância que a espécie desempenhou para o estado, não apenas pela quantidade de registros encontrados ao longo da história nas ciências e nas artes, mas também pelo seu destacado papel cultural e social associado ao povo pernambucano. O trabalho é constituído de uma introdução e cinco partes que abordam a visão dos primeiros cronistas e viajantes sobre a planta desde o século XVI; o papel da mangabeira na produção de látex nos séculos XIX e XX; o uso do fruto na culinária; a produção artística sobre a mangaba; as pesquisas com a espécie; e as considerações finais. Made available in DSpace on 2018-01-11T23:42:02Z (GMT). No. of bitstreams: 1 131001PB.pdf: 203979 bytes, checksum: 5f754e9d6798769feac50385d9bbc03e (MD5) Previous issue date: 2018-01-10

Mangabeira fruits are collected from native populations under anthropic pressure. It is therefore essential to obtain morphogenetic information to genetically improve populations, thereby preventing genetic erosion. This research was carried out in the state Amapá and aimed to verify the morphological dissimilarity between two populations, one composed of 36 native progenies and one of eight progenies from Paraiba, outlined in lattices with two replications and six plants per part. The evaluated characteristics were: agronomic value (VA), treetop circumference (CCP), plant height (ALP), estimated number of fruits per plant (NEF), fruit color (COF), average weight of 10 fruits (PMF), fruit diameter (DF), average pulp weight of ten fruits (PMP), average length of 10 fruits (CMF), average number of seeds of 10 fruits (NMS). Our results indicate that the phenotype was mainly determined by responses to environmental changes; in the correlations, inherent fruit characteristics (weight, size, and color) did not affect the VA, while biometric characters influenced VA values. Higher plants presented larger fruits and a larger diameter, bigger quantities, there is also apparent contrast, with a larger amount of smaller size; the populations are dissimilar, indicating the possibility of obtaining of diverging superior segregating.

Apocynaceae is a botanical family distributed mainly in tropical and subtropical regions of the world. In Brazil, they comprise about 90 genera and 850 species, inhabiting various types of vegetation. Within this large botanical family, the genus Hancornia is considered monotypic, with its only species Hancornia speciosa Gomes. Antihypertensive, antidiabetic, and antiviral activities are described for this species. Despite having been the target of some studies, knowledge of its chemical composition is still limited. In this study, the phenolics of H. speciosa leaves were analyzed using ultra-high performance liquid chromatography (UHPLC) coupled to Orbitrap high-resolution mass spectrometry (HRMS). As a result, 14 compounds were identified viz. protocatechuic acid, catechin, and quercetin, and another 14 were putatively identified viz. B- and C-type procyanidins, while just one compound remained unknown. From the identified compounds, 17 are reported for the first time viz. coumaroylquinic acid isomers and eriodyctiol. The results show that Hancornia speciosa can serve as source of valuable phenolics.

The mangaba (Hancornia speciosa) stands out and has a great potential for economic exploitation and researches have suggested relevant results regarding the nutritional and functional value of this food. Therefore, the objective of this study was to chemically and physicochemically characterize the mangaba fruit harvested in the Middle Araguaia region. It was evaluated the chemical composition, antioxidant activity, volatile compounds, preliminary photochemical studies of phenolic components and the cytotoxic potential of ethanolic extract fruits against Artemia salina. The mean pH, the total titratable acidity and the value for reducing sugars conclude that the mangaba possesses the characteristics appropriate for both fresh consumption and for processing. Between the mean values measured, the fruits presented high fiber content and a low caloric value. The analysis of the antioxidant potential revealed a significant activity and in the volatile compounds analysis, esters were the major class presents. The result of the phytochemical screening revealed that phenols, flavonoids and tannins were present in the ethanolic extract of mangaba and a significant activity in the cytotoxicity assay was observed.

The latex obtained from Hancornia speciosa is used in folk medicine for treatment of several diseases, such as acne, warts, diabetes, gastritis and inflammation. In this work, we describe the biocompatibility assessment and angiogenic properties of H. speciosa latex and its potential application in medicine. The physical-chemical characterization was carried out following different methodologies (CHN elemental analyses; thermogravimetric analyses and Fourier transform infrared spectroscopy). The biocompatibility was evaluated through cytotoxicity and genotoxicity tests in fibroblast mouse cells and the angiogenic properties were evaluated using the chick chorioallantoic membrane (CAM) assay model. The physical-chemical results showed that the structure of Hancornia speciosa latex biomembrane is very similar to that of Hevea brasiliensis (commercially available product). Moreover, the cytotoxicity and genotoxicity assays showed that H. speciosa latex is biocompatible with life systems and can be a good biomaterial for medical applications. The CAM test showed the efficient ability of H. speciosa latex in neovascularization of tissues. The histological analysis was in accordance with the results obtained in the CAM assay. Our data indicate that the latex obtained from H. speciosa and eluted in water showed significant angiogenic activity without any cytotoxic or genotoxic effects on life systems. The same did not occur with H. speciosa latex stabilized with ammonia. Addition of ammonia does not have significant effects on the structure of biomembranes, but showed a smaller cell survival and a significant genotoxicity effect. This study contributes to the understanding of the potentialities of H. speciosa latex as a source of new phytomedicines.

Mangaba (Hancornia speciosa Gomes) is a native fruit of great economic, cultural, and environmental importance in its areas of occurrence. Due to extractive activities and real estate pressure, the number of natural populations has considerably decreased. The propagation of the species is still one of the primary obstacles for orchard implantations, thus, studies that provide a better understanding of the dynamics of the species’ growth should be developed. This work aimed to evaluate the biometry, emergence, and initial growth of mangaba progenies (Hancornia speciosa Gomes), using the plant material from the Active Germplasm Bank of Embrapa Coastal Tablelands, located in Itaporanga d’Ajuda, SE, Brazil. Treatments consisted of progenies from 17 accessions. The experiment was carried out in a completely randomized design with four replications of 25 seeds. Number of seeds per fruit, as well as fruit and seed weight (g), length (mm), width (mm), and thickness (mm) were evaluated. For emergence and initial growth, the following variables were analyzed: percentage of emergence (PE%), emergence speed index (ESI%), survival rate (SR%), height (H), stem diameter (SD), and the number of leaves (NL). Biometric analyses of fruits and seeds revealed significant phenotypic variability among mangaba accessions. Progenies of the accessions LGP1, LGP3, LGP4, PTP4, TCP2, TCP6, ABP1, ABP2, ABP4 and BIP4 showed better results for all emergence and initial growth variables. The progenies of accessions TCP1, BIP4, CAP5 and PRP5 expressed lower emergence and survival percentages, and low vigor.

Mangabeira is a native fruit tree from Brazil, which provides a delicious fruit to taste and beneficial to health, whose information about the seedlings production will assist the entire productive chain of this important species. The aim of this research was to evaluate protected environments and substrates compositions in the formation of mangabeira seedlings. For this, two greenhouses with different screens were used, the first covered with aluminized thermo-reflective screen of 50% shading and the second with black screen of 50% shading. Inside the environments, were tested substrates derived from combinations of various proportions of bovine manure (M), soil (S), medium vermiculite (MV), super fine vermiculite (FV) and fine sand (FS). For each environment of cultivation was adopted a completely randomized design to evaluate the substrates, with five replicates of eight plants. The environments were compared by analysis of experiments groups. The results showed that both protected environments are suitable for mangabeira seedlings. In the aluminized screen is indicated for the formation of seedling in the substrate with 20% M + 30% S + 10% MV + 30% FV + 10% FS while in the black screen is indicated for seedlings in the substrate with 10% M + 30% S + 10% MV + 10% FV + 40% FS and with 10% M + 30% S + 40% MV + 10% FV + 10% FS.

Apocynaceae is a botanical family that includes between 3700 and 5100 species are distributed almost worldwide, but mainly in tropical and subtropical regions. In Brazil there are about 90 genera and 850 species, inhabiting different vegetation types. Within this large botanical family is gender Hancornia, which is considered monotypic and therefore its unique species is Hancornia speciosa Gomes, and is abundant in the northeastern cerrado. In view of this, this paper aims to study fitoquimicamente Hancornia speciosa thus contributing to the expansion of the chemical knowledge of the species. In addition to evaluating the antifungal activity of the extract and isolated compounds. For the study, the plant material after drying and pulverizing, was subjected to extraction and chromatography processes for isolation of chemical constituents. Techniques such as Cromatrografia Liquid High efficiency and Ultra-high Performance Liquid Chromatography were employed at work. The chemical structure of the substances was determined by 1H Nuclear Magnetic Resonance, 13C and dimensional comparisons with literature data. Chromatographic fractionation of the chloroform phase resulted in the isolation of caffeate acetate, the ethyl acetate phase gave the isoquecetrina beyond the narigenina, and a mix of acid 4,5-O-E- caffeoyl-quinic acid with 3,5-di –O-E- caffeoyl-quinic the butanol phase. All these compounds were first reported in Hancornia speciosa. Characterization of the extract of Hancornia speciosa leaves by Ultra-high Performance Liquid Chromatography coupled to Mass Spectrometry also provided the identification of twenty-four compounds. Since the study of the antifungal activity of the extracts provide a positive result, but compounds isolated did not provide a positive result, not being able to inhibit the growth of the strains in any concentration tested. This work contributed indicating that this species is bioprodutora phenolic compounds, as demonstrated in this phytochemical study. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Apocynaceae é uma família botânica que incluem entre 3700 e 5100 espécies, estando distribuídas praticamente no mundo todo, mas principalmente nas regiões tropicais e subtropicais. No Brasil ocorrem aproximadamente 90 gêneros e 850 espécies, habitando diversas formações vegetais. Dentro dessa grande família botânica está o gênero Hancornia, que é considerado monotípico e, por isso, sua única espécie é Hancornia speciosa Gomes, abundante no cerrado nordestino. Diante disto, este trabalho tem como objetivo estudar fitoquimicamente Hancornia speciosa contribuindo com o conhecimento químico da espécie, além de avaliar a atividade antifúngica do extrato e compostos isolados. Para realização do estudo, o material vegetal, após secagem e pulverização, foi submetido a processos de extração e cromatografia para isolamentos dos constituintes químicos. Técnicas como Cromatrografia Líquida de Alta eficiência e Cromatrografia Líquida de Eficiência Ultra Elevada foram empregadas no trabalho. A estrutura química das substâncias foi determinada por Ressonância Magnética Nuclear de 1H, 13C e bidimensionais em comparações com dados da literatura. O fracionamento cromatográfico da fase clorofórmica resultou no isolamento do cafeato de etila, da fase acetato de etila obteve-se a isoquecetrina, além da narigenina, e uma mistura do ácido 4,5-di-O-E- cafeoil-quínico com o ácido 3,5-di-O-E- cafeoil-quínico da fase butanólica. Todos esses compostos foram relatados pela primeira vez em Hancornia speciosa. A caracterização do extrato das folhas desta espécie por Cromatografia Líquida de Eficiência Ultra Elevada acoplada à Espectrometria de Massas forneceu ainda a identificação de vinte e quatro compostos. Já o estudo da atividade antifúngica do extrato forneceu um resultado positivo, porém o estudo dos compostos isolados não forneceu, não sendo capaz de inibir o crescimento das cepas em estudo em nenhuma concentração testada. Este trabalho contribuiu evidenciando que esta espécie é bioprodutora de compostos fenólicos, como demonstrado neste estudo fitoquímico.