Search

Our study aimed to assess the associations of plasma magnesium with prediabetes and type 2 diabetes (T2D) among Chinese adults. We conducted a case-control analysis of 4447 participants: 867 newly diagnosed prediabetes patients, 1475 newly diagnosed T2D patients and 2105 normal glucose tolerance (NGT) individuals. In a subsample of 599 apparently healthy individuals, we measured plasma hs-CRP levels to examine their relation to plasma magnesium. Plasma magnesium and hs-CRP were measured by inductively coupled plasma mass spectrometry and an enzyme-linked immunosorbent assay, respectively. Plasma magnesium decreased from NGT to prediabetes to T2D, and was inversely associated with prediabetes and T2D. After adjustment for potential confounding factors, the ORs from the lowest to highest quartiles of plasma magnesium were 1, 0.57 (95%CI, 0.44–0.74), 0.49 (0.37–0.65) and 0.51 (0.37–0.70) for prediabetes, and 1, 0.26 (0.20–0.33), 0.15 (0.12–0.20) and 0.15 (0.11–0.20) for T2D. Consistently, plasma magnesium was inversely correlated with plasma hs-CRP in our subsample analysis; the geometric mean hs-CRP concentration for ascending quartiles of plasma magnesium were 1.29 (1.06–1.57), 1.16 (0.95–1.41), 1.00 (0.81–1.22), and 0.71 (0.58–0.88) mg/l. Plasma magnesium was independently and inversely associated with prediabetes and T2D in Chinese adults.

Drosophila melanogaster is a well-established model organism that is widely used in genetic studies. This species enjoys the availability of a wide range of research tools, well-annotated reference databases and highly similar gene circuitry to other insects. To facilitate molecular mechanism studies in Drosophila, we present the Predicted Drosophila Interactome Resource (PDIR), a database of high-quality predicted functional gene interactions. These interactions were inferred from evidence in 10 public databases providing information for functional gene interactions from diverse perspectives. The current version of PDIR includes 102 835 putative functional associations with balanced sensitivity and specificity, which are expected to cover 22.56% of all Drosophila protein interactions. This set of functional interactions is a good reference for hypothesis formulation in molecular mechanism studies. At the same time, these interactions also serve as a high-quality reference interactome for gene set linkage analysis (GSLA), which is a web tool for the interpretation of the potential functional impacts of a set of changed genes observed in transcriptomics analyses. In a case study, we show that the PDIR/GSLA system was able to produce a more comprehensive and concise interpretation of the collective functional impact of multiple simultaneously changed genes compared with the widely used gene set annotation tools, including PANTHER and David. PDIR and its associated GSLA service can be accessed at http://drosophila.biomedtzc.cn.

Objective: The relationship between the waiting time of postoperative radiotherapy and the prognosis of patients with high-grade glioma is still inconclusive, and we addressed this issue through a systematic review and meta-analysis. Methods: Twenty studies published between 1975 and 2019 about waiting times (WT) of radiotherapy with high-grade glioma were retrieved for meta-analysis.The meta-analysis was performed by converting the effect sizes of different WT into regression coefficients (β) and standard error (SE) to indicate the daily impact of delay on OS. Results: A total of 8462 high-grade glioma patients were included in the 20 studies, and no correlation between WT delay and OS was found in the unadjusted model through meta-analysis (HR=1, 95%CI=0.99-1.01, p=0.962). Meta-regression was used to adjust for other prognostic factors and no clear evidence of the relationship between WT delay and OS was found. Conclusion: This meta-analysis suggests that there is no clear evidence for the effect of delayed radiotherapy on OS with high-grade glioma patients.

Pressurized exploitation is one of the important measures to ensure the continuous and efficient exploitation of gas field by reducing wellhead pressure and increasing external transportation pressure in the later period of gas field exploitation. As a typical low permeability gas field, the south area of yulin gas field has entered a decline period. The surface gathering and transportation system has been unable to adapt to the development of middle and low pressure gas reservoirs. How to carry out pressurized mining economically and effectively has become an urgent problem to be solved. In view of difficulties in gas field development, key parameters such as the timing, mode, scale and minimum wellhead pressure of pressurized gas field exploitation were studied by taking production, gathering and transportation system and economic factors into consideration. The key technology of the pressurization of low permeability gas reservoir in southern yulin gas field is formed.

Rattus norvegicus, or the rat, has been widely used as animal models for a diversity of human diseases in the last 150 years. The rat, as a disease model, has the advantage of relatively large body size and highly similar physiology to humans. In drug discovery, rat models are routinely used in drug efficacy and toxicity assessments. To facilitate molecular pharmacology studies in rats, we present the predicted rat interactome database (PRID), which is a database of high-quality predicted functional gene interactions with balanced sensitivity and specificity. PRID integrates functional gene association data from 10 public databases and infers 305 939 putative functional associations, which are expected to include 13.02% of all rat protein interactions, and 52.59% of these function associations may represent protein interactions. This set of functional interactions may not only facilitate hypothesis formulation in molecular mechanism studies, but also serve as a reference interactome for users to perform gene set linkage analysis (GSLA), which is a web-based tool to infer the potential functional impacts of a set of changed genes observed in transcriptomics analyses. In a case study, we show that GSLA based on PRID may provide more precise and informative annotations for investigators to understand the physiological mechanisms underlying a phenotype and lead investigators to testable hypotheses for further studies. Widely used functional annotation tools such as Gene Ontology (GO) analysis, and Database for Annotation, Visualization and Integrated Discovery (DAVID) did not provide similar insights. Database URL: http://rat.biomedtzc.cn.

This study aimed to evaluate neonatal surgical outcomes of patients diagnosed with complex congenital heart disease (CHD) during pregnancy and treated by the newly initiated "perinatal integrated diagnosis and treatment program (PIDTP)".We reviewed clinical data of 207 neonates (surgical age ≤ 28 days) who underwent cardiac surgeries in a single center from January 2017 to December 2018, including 31 patients with referrals from the "PIDTP" (integration group) and 176 patients with routine referral treatment (non-integrated group).In the integration group, median admission age was 0 days and median age at surgery was 4 days. In the non-integrated group, median admission age was 8 days (P = 0.001) and median age at surgery was 13 days (P = 0.001). The emergency surgery rate in patients with duct-dependent defects was 36% in the integration group and 59% (P = 0.042) in the non-integrated group, respectively. The in-hospital mortality was 16% in the integration group and 14% (P = 0.78) in the non-integrated group. The 2-year cumulative survival rate after surgery was 83.9% ± 6.6% in the integration group and 80.3% ± 3.1% (P = 0.744) in the non-integrated group. According to multivariable regression analysis, independent risk factors for early mortality of overall neonatal cardiac surgery were low body weight, high serum lactate level, postoperative extracorporeal membrane oxygenation (ECMO) support and prolonged cardiopulmonary bypass (CPB) time.PIDTP shortens the postnatal transit interval, reduces the emergency operation rate of neonatal critical CHD, and provides better preoperative status for surgery. Patients treated by the PIDTP tend to have more complicated anatomical deformity and a greater requirement for the operation and postoperative management, but early outcome and follow-up prognosis are satisfactory.

Saccharomyces cerevisiae, budding yeast, is a widely used model organism and research tool in genetics studies. Many efforts have been directed at constructing a high-quality comprehensive molecular interaction network to elucidate the design logic of the gene circuitries in this classic model organism. In this work, we present the yeast interactome resource (YIR), which includes 22,238 putative functional gene interactions inferred from functional gene association data integrated from 10 databases focusing on diverse functional perspectives. These putative functional gene interactions are expected to cover 18.84% of yeast protein interactions, and 38.49% may represent protein interactions. Based on the YIR, a gene set linkage analysis (GSLA) web tool was developed to annotate the potential functional impacts of a set of transcriptionally changed genes. In a case study, we show that the YIR/GSLA system produced more extensive and concise annotations compared with widely used gene set annotation tools, including PANTHER and DAVID. Both YIR and GSLA are accessible through the website http://yeast.biomedtzc.cn.

To investigate the association between SNPs in human IGF2/H19 gene locus and epithelial ovarian cancer (EOC) risk, we performed a case-control study in 422 individuals (219 EOC patients and 203 cancer-free controls). Four SNPs (rs2525885, rs2839698, rs3741206, rs3741219) were found to be related with EOC risk. Specifically, the minor allele C of rs2525885 and allele A of rs2839698 was associated with elevated EOC genetic susceptibility under both dominant and recessive models (TC + CC vs TT: adjusted OR: 1.61, P = .031; CC vs TT + TC: adjusted OR: 4.87, P = .014; GA + AA vs GG: adjusted OR: 1.63, P = .023; AA vs GG + GA: adjusted OR: 2.43, P = .007). For rs3741206, the genotype TC + CC was associated with a significant decrease in EOC risk with the TT genotype as reference in a dominant genetic model (adjusted OR: 0.44, P = .003), while for rs3741219, genotype AA was associated with a 59% decrease in EOC risk only in the recessive model (adjusted OR: 0.41, P = .038). In the stratified analysis, an increased risk associated with the variant genotypes was observed in only subjects aged >47 years for rs2525885 (adjusted OR = 2.04, P = .024), rs2839698 (adjusted OR = 2.50, P = .047) and rs3741206 (adjusted OR = 0.37, P = .009), respectively. What's more, the TC + CC genotype of rs2525885 was significantly associated with advanced FIGO stage (III vs II, adjusted OR = 2.73, P = .040).

Prostate cancer (PCa) is the most common malignancy in men and in the absence of any effective treatments available. For the development of potential anticancer agents, 24 kinds of naftopidil-based arylpiperazine derivatives containing the bromophenol moiety were synthesized and characterized by using spectroscopic methods. Their pharmacological activities were evaluated against human PCa cell lines (PC-3 and LNCaP) and a1-adrenergic receptors (a1-ARs; α1a, α1b, and α1d-ARs). The structure–activity relationship of these designed arylpiperazine derivatives was rationally explored and discussed. Among these derivatives, 3c, 3d, 3h, 3k, 3o, and 3s exhibited the most potent activity against the tested cancer cells, and some derivatives with potent anticancer activities exhibited better a1-AR subtype selectivity than others did (selectivity ratio > 10). This work provided a potential lead compound for the further development of anticancer agents for PCa therapy.

Inflammatory myofibroblastic tumor (IMT), a rare sarcoma, is primarily treated via resection of the mass. However, there is no standard treatment for recurrence or unresectable tumors. Almost 50% of IMTs carry ALK gene rearrangement that can be treated using ALK inhibitors, but therapeutic options for ALK-negative tumors are limited. This report describes a woman aged 22 years with unresectable ALK-negative IMT. Next-generation sequencing revealed a TFG-ROS1 fusion, and she had a partial response to the ROS1 inhibitor ceritinib. This report provides the first published demonstration of a patient with IMT with ROS1 fusion successfully treated using ceritinib. Our study suggests that targeting ROS1 fusions using the small molecule inhibitor shows promise as an effective therapy in patients with IMT carrying this genetic alteration, but this requires further investigation in large clinical trials.

Twenty-one polycyclic polyprenylated acylphloroglucinols, including three new compounds named as hyperichoisins A (

Background Preeclampsia (PE) is a severe pregnancy complication and is an important cause for maternal and child death, premature delivery, and limited intrauterine growth and development. The aim of this study was to investigate the role of NGAL and cystatin C, alone and in combination, for early prediction of PE at 10 - 14 weeks of gestation. Methods Serum levels of NGAL and cystatin C were assessed in women at 10 - 14 weeks of gestation who subsequently developed PE (n = 128) and normal pregnancy outcome (n = 183). Comparison of clinical characteristics, NGAL, and cystatin C levels between normal pregnancy and PE groups were analyzed using Mann-Whitney test. The receiver operating characteristic curve (ROC curve) was used to analyze the value of serum NGAL and cystatin C levels in predicting PE. Results The levels of cystatin C and NGAL in the serum were significantly higher in the PE group [0.64 mg/L (0.52 - 0.78)] and [34.9 ng/mL (24.4 - 55.2), respectively] than in the normal pregnancy group [0.56 mg/L (0.49 - 0.65)] and [20.2 ng/mL (13.8 - 26.9), respectively]. ROC curve analysis showed that serum NGAL levels predicted the area under the curve in the PE period 0.739 (95% CI: 0.618 to 0.860). Serum cystatin C levels predicted the area under the curve in the PE period 0.722 (95% CI: 0.592 to 0.853). The combination of serum NGAL and cystatin C levels predicted the area under the curve in the PE period 0.877 (95% CI: 0.811 to 0.943). Conclusions NGAL and cystatin C levels in serum appear to be ideal biomarkers for PE prediction at 10 - 14 weeks. The combination of NGAL and cystatin C will also be more valuable in discriminating patients at risk of developing PE from other pregnancy complications early in gestation.

To investigate the effects of β-carboxyethyl germanium sequioxide (Ge-132) and germanium dioxide (GeO2) on improving salt tolerance of evening primrose (Oenothera biennis L.), seed germination, seedling growth, antioxidase and malondialdehyde (MDA) were observed under treatments of various concentrations (0, 5, 10, 20, 30 μM) of Ge in normal condition and in 50 mM NaCl solution. The results showed that both Ge-132 and GeO2 treatments significantly increased seed germination percentage and shoot length in dose-dependent concentrations but inhibited early root elongation growth. 5–30 μM Ge-132 and 10, 20 μM GeO2 treatments could significantly mitigate even eliminate harmful influence of salt, representing increased percentage of seed germination, root length, ratio between length of root and shoot, and decreased shoot length. These treatments also significantly decreased peroxidase (POD) and catalase (CAT) activities and MDA content. The mechanism is likely that Ge scavenges reactive oxygen species ...

TPS151 Background: The standard third-line treatment of mCRC is regofenib, fruquintinib or TAS-102. However, the efficacy was not satisfied. Because VEGF and EGFR share the downstream signal pathway, targeting these two pathways may have synergistic efficacy. Preclinical studies have confirmed the effect of the combination of regorafenib and cetuximab. The subsequent phase Ⅰb study in advanced cancer showed the combination is effective. Fruquintinib is the same anti-VEGF drugs and was approved in China. Our department has treated one patient of advanced colorectal cancer, no standard regimen after third-line treatment. The efficacy is SD and PFS time exceeded 6 months. Therefore this study explores the safety and efficacy of fruquintinib combined with cetuximab. Methods: This is a single-center, non-random, prospective, open exploratory study. Eligible pts were diagnosed as advanced RAS/BRAF wild-type colorectal cancer and had received at least two prior regimens of standard therapies. The purpose of phase Ⅰ is to confirm the safety and the appropriate dose of fruquintinib combined with cetuximab. The phase Ⅱa investigate the safety and the efficacy of fruquintinib combined with cetuximab. Phase I: The dose of cetuximab is 500mg/m2 every two weeks which is clinically tolerable, so the dose need not be adjusted. fruquintinib is taken once daily for the first 21 days of each 28-day cycle and the dose adjustment is required due to the side effects. The standard dose of fruquintinib is 5mg/d, but the side effects are serious. The dose of 3mg/d is the minimum effective dose, therefore the initial dose of fruquintinib starts from 4mg, and three patients are enrolled. If DLT appeared in less than three patients, the dose elevated to 5mg/d. Three patients were enrolled in the 5mg/d dose group. If DLT appeared in less than three patients, the right dose of fruquintinib was 5mg/d. If DLT appeared in all three patients of the 5mg/d, fruquintinib was declined to 4mg/d combined with cetuximab to carry out a phase Ⅱa study. Fruquintinib initial dose is 4mg/d. If DLT was present in all three patients, the dose was reduced to 3mg/d. The standard dose of fruquintinib (3mg/d) was determined to be MDT, and the subsequent phase Ⅱa study was conducted by giving fruquintinib 3mg/d. The phase Ⅱa study is cetuximab 500mg/m2 biweekly combined with fruquintinib according to the confirmed dose in phase Ⅰ, taking it once daily for the first 21 days of each 28-day cycle. The number of cases is 20.Response assessment via CT/MRI is to be done q8 wks (RECIST 1.1). Continue until disease progression or unacceptable toxicity. The primary objective is to estimate safety, grade 3/4 adverse, and DLT. Secondary objectives include ORR, PFS, and OS. Final analysis to be done 6 mo after enrollment of the final pt. Clinical trial information: ChiCTR2000038227 . Research Sponsor: None

A phytochemical investigation on the 75% EtOH extract of the aerial parts of

Twenty-one polycyclic polyprenylated acylphloroglucinols, including three new compounds named as hyperichoisins A (3), B (14) and C (21), were isolated from the aerial parts of Hypericum choisianum. The structures of those new compounds were elucidated by analysis of mass, NMR data, and chiroptical properties. A bioassay showed that otogirinin B had significant inhibitory effect on cell proliferation of A549.

A novel scaffold of arylpiperazine derivatives was discovered as potent androgen receptor (AR) antagonist through rational drug designation based on our pre-work, leading to the discovery of a series of new antiproliferative compounds. Compounds 10, 16, 27, 29 and 31 exhibited relatively strong antagonistic potency against AR and exhibited potent AR binding affinities, while compounds 5, 6, 10, 14, 16, 19, 21, 27 and 31 exhibited strong cytotoxic activities against LNCaP cells (AR-rich) as well as also displayed the higher activities than finasteride toward PC-3 (AR-deficient) and DU145 (AR-deficient). Docking study suggested that the most potent antagonist 16 mainly bind to AR ligand binding pocket (LBP) site through hydrogen bonding interactions. The structure-activity relationship (SAR) of these designed arylpiperazine derivatives was rationally explored and discussed. These results indicated that the novel scaffold compounds demonstrated a step towards the development of novel and improved AR antagonists, and promising candidates for future development were identified.

Inhibin B, a glycoprotein produced predominantly by Sertoli cells and preferentially suppressing the production and secretion of follicle-stimulating hormone (FSH) in the pituitary, is closely related to spermatogenesis. Varicocele is the abnormal dilatation and tortuosity of the pampiniform plexus veins, which may contribute to spermatogenic dysfunction and male infertility. More and more evidence has shown that the level of serum inhibin B is negatively correlated with the severity of varicocele. Determination of the inhibin B level may help assess the severity of spermatogenic dysfunction of the patient and predict the outcomes of varicocele repair and therefore has a potential application value in the diagnosis and treatment of varicocele.抑制素B主要由睾丸支持细胞合成的可特异性地抑制垂体FSH合成与分泌的一种糖蛋白激素，与生精功能密切相关。精索静脉曲张指精索内蔓状静脉丛的异常迂曲、扩张，可以引起睾丸生精功能障碍，是导致男性不育常见的病因。越来越多的证据表明，血清抑制素B水平与精索静脉曲张程度呈负相关，检测血清抑制素B水平能评估精索静脉曲张患者生精功能的损害程度，并可预测精索静脉曲张术后的疗效，在精索静脉曲张疾病的诊疗中有潜在的应用价值。.

With the development of high-performance fighters, the function of pilot helmets is also constantly upgrading, and helmet-mounted display (HMD) has become a future development trend. To solve the inter-restriction between the eye position control and the pressure comfort under high overloads of pilot helmets, this paper optimizes the helmet design by building a head-helmet finite element model: (1) Under a 10 G overload, the deformation of helmet soft liner should not exceed 2 mm; and (2) The combination of liner materials used in various areas should satisfy the requirements of most comfortable head pressure distribution (Pfront: Ptop: Pside: Pback = 2:2:1:3). The study on 46 different combinations of materials finds that the helmet can meet both the eye position maintenance requirement under overloads and the requirement of comfortable pressure distribution when linear elastic materials with Young's modulus of 1000 pa, 55000 pa and 65000 pa are used for the side, top and front parts of the head, and nonlinear elastic hyperfoam material with two-fold stress variation is used for the head back area.

Objective: The aim of this study was to identify heart failure (HF)-specific circulating micro-RNAs (miRNA), and examine whether the selected miRNAs correlate with myocardial fibrosis and are reflective of the incidence of adverse cardiovascular events in patients with stage C or D HF. Methods: Circulating miRNAs which were expressed in end-stage HF patients and matched healthy controls were detected by microarray analysis and validated by quantitative real-time polymerase chain reaction. Multivariate Cox regression analysis was performed to determine whether the selected circulating miRNAs could be prognostic factors in HF patients. Results: In a cohort of 7 healthy controls and 9 patients with stage C or D HF, 7 miRNAs were differentially expressed. These miRNAs were further investigated in a second cohort of 80 patients with stage C or D HF and 30 healthy controls. Only miR-197-5P correlated with fibrosis as seen in cardiac magnetic resonance imaging in patients under the age of 50 years with stage C or D HF (r = 0.42, p = 0.008). Multivariate analyses revealed that miR-197-5P was also a risk factor for composite endpoint events in patients under the age of 50 years with stage C or D HF. Conclusion: miR-197-5P is a circulation miRNA that correlates with MF and adverse cardiac events in HF patients under the age of 50 years.

Zinc (Zn) highly concentrates in the brain and plays a key role in memory formation and learning processes. Zn deficiency results in cognitive impairments, memory deficits, alterations of neuropsychological behavior, and motor development. Although Zn-enriched yeast (ZnY) is widely used for dietary fortification and supplementation of Zn, the effect of ZnY on cognition still remains unclear. The purpose of the study was to investigate the effect of ZnY on behavior in Zn-deficient and Zn-sufficient rats. Three-week-old rats were fed low Zn diets for 145 days to establish Zn-deficient rats. ZnY was orally administered to Zn-deficient rats at three dose levels of 1, 2, and 4 mg Zn/kg/day for 55 days. Rat appearance, body weight, plasma and brain Zn, Morris water maze test, and step-through passive avoidance test were observed. Compared to Zn-sufficient rats, body weight gain, plasma zinc level, resident time, and step-through time in Zn-deficient rats were significantly lower. Zn deficiency impaired functions of learning and memory, while ZnY as a plausible therapeutic intervention alleviated the cognitive impairments caused by Zn deficiency.

The surface potentials and dynamic charging properties of polymers subjected to electron irradiation in scanning electron microscopy are investigated by using a novel method applied in parallel with simulation and experimental methods. We perform our simulation through a parallel computing method with various microscopic parameters. The results show that under the given microscopic parameters, the charging time concomitantly decreases as the film thickness, electron mobility, trap cross and recombination rate increase. The surface potential decreases as electron energy, beam current density, capture cross section, sample thickness and electron-hole recombination rate increase and as electron mobility decreases. Charging time increases with increasing beam density, sample thickness, recombination rate and change in capture cross-section, and decreases with increasing electron mobility. The primary-electron energy exhibits an inflection point at 35 keV. This study offers an intuitive analytical and measurement method for understanding microscopic charge characteristics in electron-based surface microscopy.

As a kind of globally widely distributed soil microorganism, arbuscular mycorrhizal (AM) fungi form the symbiosis with the majority of land plants, which is important for plant nutrition and ecosystem functioning. Few vascular species are considered to be nonmycorrhizal, especially those within the families Amaranthaceae, Chenopodiaceae, Carophyllaceae and Brassicaceae. At present, the interactions between these non-host plants and AM fungi are few and scattered, lacking systematic summary. In this paper, the type of non-host plants, the reason of low mycotrophy, and the effect of AM network formed by neighbor plants on AM fungi colonization on the non-host, and the possible interaction between AM fungi and non-host, as well as the material exchange between plants and AM fungi and their possible ecological functions were reviewed in order to give some new ideas on the function of the nonmycorrhizal plants in the fragile ecosystems.丛枝菌根(AM)真菌作为一类在全球分布广泛的土壤微生物,能够与陆地上大多数的维管植物形成专性共生关系,对于植物营养吸收和生态系统功能具有重要作用.而较少量的维管植物如苋科、黎科、石竹科、十字花科等植物被认为是非菌根植物.目前,对于这些非菌根植物与AM 真菌之间的相互作用关系研究少且分散,缺乏系统总结.本文综述了非菌根植物的类型以及低侵染的原因,邻体植物形成的菌丝网络对AM真菌侵染非菌根植物的影响,并探讨AM真菌和非菌根植物之间可能存在的相互作用,以及植物-AM真菌之间的物质交换及可能存在的生态功能,旨在为进一步发挥非菌根植物在脆弱生态系统的功能潜力提供新思路.

In order to improve the reliability and stability of the automobile suspension system, the important parts of automobile suspension control arm is studied. Reverse engineering technology is applied to reverse modeling according to the characteristics of complicated suspension control arm shape. At the same time, the automobile suspension control arm is analyzed under load condition to ensure that the automobile suspension control arm is safe in the process of driving. The control arm meshing and strength analysis are completed by ANSYS analysis software. Finally, ANSYS fatigue analysis module is used to verify that the automobile suspension control arm can meet the requirement of fatigue strength.

In this paper, based on the requirements of standards system of distribution automation, standardized testing method and testing process of performance index of distribution automation system (DAS) is proposed according to the characteristics of implementation, and the technology of automatic test is studied. The paper also brings forward a set of feasible evaluation method and system of DAS to ensure the safe and stable running of distribution automation (DA), combining with a common information model (CIM) import and export function test based on feedback principle and a function test of feeder automation based on predefined fault model.

In order to have a real-time 3-D imaging of the attitude motion simulator model and to design a multiple views interface in the display and control software of the attitude motion simulator, Firstly, 3D Model of the attitude motion simulator model has been transformed to STL file; Secondly, the functions on the VC++6.0 platform have been written to read the STL file, and 3-D Image display from STL file has been realized by the programming technique of the triangle mesh renderingin OpenGL.Meanwhile, the real-time synchronization has been realized between the 3-D Imageand real angle of attitude motion simulator model.Finally, in order to meet the demands ofcontrol and display software, a multiview control interface in which a real-time data interchange amongthe multiple views is designed.

The objective of this study was to determine whether cells from human pulp can be transduced to express the antimicrobial peptide--human β-defensin-2 (HBD-2). Primary human pulp cells and gingival fibroblasts from normal tissue, as well as two mouse cell lines (NIH 3T3 and AT-84) and a human cell line SCC-9 were transduced with a retroviral vector carrying HBD-2 cDNA. ELISA and Northern blot analyses were performed to detect HBD-2 expression by these transduced cells. Antimicrobail assays using recombinant HBD-2 were performed on two caries-associated bacteria Streptococcus mutnas and Lactobacillus acidophilus. The results showed that transduced pulp cells secreted 62.4 ± 27.2 ng/3 days of HBD-2, which was comparable to that by NIH 3T3 (78.0 ± 14.1 ng/4 days), and higher than those by gingival fibroblasts (17.9 ± 7.9 ng/3 days), AT-84 (2.6 ± 1.0 ng/3 days), and SCC-9 (47.6 ± 9.9 ng/3 days). Northern blot analysis showed that the levels of HBD-2 mRNA expression correlated with their protein secretion levels. There was approximately 50% reduction of growth when S. mutans and L. acidophilus were exposed to HBD-2 at 1 µM. Pulp cells appear to be suitable for HBD-2 transduction using retroviral vectors, suggesting a potential for use in controlling pulpal infections.

In the fields of traffic modeling, objects (i.e., various vehicles) are usually represented using multimodal features. However, two problems remain unsolved on how to utilize these multimodal features better: 1) The missing features because of noise and 2) The curse of dimensionality. In this paper, we addressed these two problems by integrating the multimodal features on the Grassmann manifold. By defining grouping criteria on the multimodal features, the feature vectors are grouped into a set of subspaces, and are further treated as a point on the Grassmann manifold. To deal with the missing features L2-Hausdorff distance, a metric that compares multimodal feature vectors of different number of subspaces, is computed first. And then a kernel matrix is computed. Furthermore, based on the kernel matrix, we proposed a supervised as well as an unsupervised feature selection criterion to obtain t h e representative features on in the Reproducing Kernel Hilbert Space (RKHS). This has alleviated the curse of dimensionality to a significant extent. Experimental results on three different multimodal datasets show that the proposed feature integration technique can outperform the state-of-the-art methods

The photophysics and photochemistry of thioacetamide (CH3CSNH2) after excitation to the S2 electronic state were investigated by using resonance Raman spectroscopy in conjunction with the complete active space self-consistent field (CASSCF) method and density functional theory (DFT) calculations. The A-band resonance Raman spectra in acetonitrile, methanol, and water were obtained at 299.1, 282.4, 266.0, 252.7, and 245.9 nm excitation wavelengths to probe the structural dynamics of thioacetamide in the S2 state. CASSCF calculations were done to determine the transition energies and structures of the lower-lying excited states, the conical intersection points CI(S2/S1) and CI(S1/S0), and intersystem crossing points. The structural dynamics of thioacetamide in the S2 state was revealed to be along eight Franck-Condon active vibrational modes ν15, ν11, ν14, ν10, ν8, ν12, ν18, and ν19, mostly in the CC/CS/CN stretches and the CNH8,9/CCH5,6,7/CCN/CCS in-plane bends as indicated by the corresponding normal mode descriptions. The S2 → S1 decay process via the S2/S1 conical intersection point as the major channel were excluded. The thione-thiol photoisomerization reaction mechanism of thioacetamide via the S2,FC → S'1,min excited state proton transfer (ESPT) reaction channel was proposed.

The concept of smart substations has been widely used in power systems. Since the variation in interfaces and communication manner, the operating& maintenance methods for smart stations have changed a lot compared with the traditional substations. In this paper , a training system based on virtual substation primary equipment combined with real secondary devices is developed for accident management and operating work in smart substations. The training system is also used to test the relay protection algorithms、Ethernet switch functions、fault recorder actions ,and so on.

Peng-Fei Fan, Kai He, Xing Chen, Alejandra Ortiz, Bin Zhang, Chao Zhao, Yun-Qiao, Hai-Bo Zhang, Clare Kimock, Wen-Zhi Wang, Colin Groves, Samuel T. Turvey, Christian Roos, Kristofer M. Helgen, Kristofer M. Helgen, Xue-Long Jiang (2017): Description of a new species of Hoolock gibbon (Primates: Hylobatidae) based on integrative taxonomy. American Journal of Primatology 79: 1-15, DOI: 10.1002/ajp.22631

Background: Rheumatic heart disease (RHD) is a predominant health concern in developing countries. The aim of this meta-analysis was to evaluate the outcomes of mitral valve (MV) repair in patients with RHD, and identify predictors that may postoperatively affect treatment outcome. Methods: A meta-analysis of eligible studies assessing patients undergoing MV repair with RHD and reporting the outcomes of MV repair, including 30-day mortality and long-term follow-up survival, MV reoperation rate and postoperative adverse events. Relevant English articles were searched up to 1 March, 2017 in Web of Science, PubMed, Google Scholar, Cochrane Library, EmBase, Elsevier, and Science Direct. Selected studies should meet all inclusion criteria, and underwent data extraction. Results: A total of ten studies with 2,770 patients met all inclusion criteria, and were selected for assessment. Pooled analysis showed that 30-day mortality in patients with rheumatic MV disease after MV repair surgery was 1.9%, 95% confidence interval (CI) (0.8–2.9%); long-term survival was 97.3%, 95% CI (95.9–98.6%), and a freedom from reoperation rate of 93.6%, 95% CI (91.4–95.9%) was obtained; freedom from adverse events was 97.5%, 95% CI (95.2–99.8%). Conclusions: The outcome of rheumatic MV repair is outstanding in terms of low early mortality, high long-term survival and freedom from valve-related complications, which may be very common in patients after rheumatic MV replacement; meanwhile, MV reoperation rate after initial surgery is acceptable. Surgeons may try to repair MV in RHD when it is feasible

The main objective of the current research work was to investigate the antitumor effects of papaverine in PC-3 human prostate cancer cells along with testing its toxicity in the normal human fibroblast (NHF) cells.The cytotoxic effects of papaverine were examined by the MTT cell viability assay. Flow cytometry using annexin V-FITC/PI was used to study the effects on apoptosis, including its quantification. Effects on cell cycle progression were analyzed by flow cytometry while as effects on apoptosis-related proteins, NF-kB and PI3K/Akt pathways were estimated by Western blot assay.The results indicated that papaverine could induce significant, highly selective and dose-dependent cytotoxic effects in PC-3 cells without causing too much toxicity in normal cells. Papaverine also led to induction of early and late apoptosis along with inducing sub-G1 cell cycle arrest in a dose-dependent manner. Papaverine induced a dose-dependent reduction in the expression levels of Blc-2 proteins and a dose-dependent increase in the expression levels of Bax protein. The expression levels of NF-kB were decreased markedly in comparison to the untreated control. Papaverine treatment also led to a dose-dependent downregulation of PI3K and phospho-Akt expression.Papaverine showed selective antitumor properties against PC-3 human prostate cancer cells by inducing early and late apoptosis, sub-G1 cell cycle arrest, modulation of apoptosis-related proteins like Bcl-2, Bax, Bid, XIAP and cytochrome C along with downregulation of NFkB, PI3K/Akt signalling pathway.

The relationship between microscopic parameters and polymer charging caused by defocused electron beam irradiation is investigated using a dynamic scattering-transport model. The dynamic charging process of an irradiated polymer using a defocused 30 keV electron beam is conducted. In this study, the space charge distribution with a 30 keV non-penetrating e-beam is negative and supported by some existing experimental data. The internal potential is negative, but relatively high near the surface, and it decreases to a maximum negative value at z = 6 μm and finally tend to 0 at the bottom of film. The leakage current and the surface potential behave similarly, and the secondary electron and leakage currents follow the charging equilibrium condition. The surface potential decreases with increasing beam current density, trap concentration, capture cross section, film thickness and electron–hole recombination rate, but with decreasing electron mobility and electron energy. The total charge density increases with increasing beam current density, trap concentration, capture cross section, film thickness and electron–hole recombination rate, but with decreasing electron mobility and electron energy. This study shows a comprehensive analysis of microscopic factors of surface charging characteristics in an electron-based surface microscopy and analysis.

Engine energy-saving technology is the key direction of passenger car energy-saving. This paper has built energy-saving potential model, which could forecast the potential of engine energy-saving technology in 2015 and 2020 under such two constraints: overall target requirement of fuel consumption and curb weight range. Afterwards, the model was used to calculate energy-saving potential of narrow sense passenger cars (including basic car + SUV + MPV), which were equipped with single turbo charging technology, single GDI technology and the above two kinds of technologies integration. The research results will provide decision-making support for enterprises’ technology selection and product development.

a Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, The Key Laboratory of Remodeling-related Cardiovascular Diseases, Ministry of Education, Department of Atherosclerosis, 2 Anzhen Road, ChaoYang District, Beijing 100029, China b Department of Radiology, The China–Japan Friendship Hospital, Beijing 100029, China c Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, 2 Anzhen Road, Chaoyang District, Beijing 100029, China d Department of Imaging, Beijing Anzhen Hospital, Capital Medical University, 2 Anzhen Road, Chaoyang District, Beijing 100029, China e Department of Emergency, Beijing Anzhen Hospital, Capital Medical University, 2 Anzhen Road, Chaoyang District, Beijing 100029, China f National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun 130024, Jilin, China g Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China

Objective The purpose of the present study was to assess the efficacy of the long-term results after video-assisted pulmonary vein isolation and left atrial appendage excision for lone atrial fibrillation (AF) and to determine the most significant risk factors for the long-term results. Methods From December 2006 to December 2012, 332 consecutive patients with lone AF underwent minimally invasive surgical ablation at our center. Of the 332 patients, 91, who had undergone video-assisted pulmonary vein isolation >5 years earlier, were evaluated in the present study (48 with paroxysmal AF, 21 with persistent AF, and 22 with long-standing persistent AF). The median follow-up period was 66 months. The primary endpoint was the success rate of video-assisted pulmonary vein isolation, defined as the absence of any atrial arrhythmia recurrence lasting >30 seconds at the clinical visit and on the electrocardiogram or long-term cardiac rhythm recording after discharge. Results During the follow-up period, 1 patient (1.1%) experienced a stroke and 4 (4.4%) died of noncardiac disease. At the 5-year follow-up point, 43 of 78 patients (55.1%) were in normal sinus rhythm. Of the 39 patients with paroxysmal AF and 39 with nonparoxysmal AF, 27 (69.2%) and 16 (44.1%) were in normal sinus rhythm, respectively. The results of the univariate and multivariate analyses of the preoperative risk factors for AF recurrence showed a left atrial diameter of ≥44 mm (hazard ratio, 5.56; 95% confidence interval, 1.68-18.387; P = .005) and an AF duration of ≥31.5 months (hazard ratio, 3.67; 95% confidence interval, 1.50-8.95; P = .004) were the most significant independent risk factors. Conclusions Patients with lone AF with a large preoperative left atrial diameter and long AF duration will not be suitable for video-assisted pulmonary vein isolation alone and might need to undergo ablation of the lesions.

Purpose The aims of this study were to present an uncommon intracranial germinoma manifesting as skull base extension and analyze its clinical characteristics to give valuable insight into such uncommon radiologic variant. Methods This is a clinical study of a 15-year-old girl with intracranial germinoma manifesting as skull base extension. Clinical characteristics, magnetic resonance imaging scan observations, pathologic findings, and flow of the treatment procedure were presented and analyzed. Results She had a 5-month history of diuresis and diplopia. magnetic resonance imaging observation displayed a neoplasm located in the right-side central skull base and suprasellar area with wide extension into the cavernous sinus, intraorbital region, ethmoidal sinus, sphenoid sinus, and pituitary fossa. After administration of contrast medium, strong and heterogeneous enhancement of the mass was observed, with a dural tail sign along the right cerebellar tentorial. Right pterional approach was performed, and intraoperative histologic examination suspected the diagnosis of germinoma; partial resection was achieved, and postoperative radiotherapy was administered. Cranial nerve palsy improved greatly 6 months postoperatively. Conclusions Although highly unusual, germinoma should be included in the differential diagnosis of all masses with extension along the midline region of skull base, especially when it happens in young female patients.

In this work, we studied the catalytic kinetics of the alliin/alliinase system using a fiber-optic in situ monitoring system (fiber-optic drug dissolution test system, FODT) to explore the application of a fiber-optic sensor analysis technique in the process of monitoring catalytic kinetics. According to Lambert–Beer's law and with the assistance of the computational relationship of alliin, allicin and pyruvate, we established two mathematical models, including obtaining the absorptivity of each substance in the reaction and looking at the products as a whole. According to the UV spectra of alliin, alliinase, sodium pyruvate, an allicin solution and the analyte corresponding to the allicin peak extracted from an HPLC/PDA chromatogram, an optical probe with a 5-mm gap was chosen for the measurement, and 230 nm was chosen as the detection wavelength during the catalysis reaction. Then, we determined the value of every parameter in the mathematical model at 230 nm and recorded the information into the workstation. A certain concentration of alliinase was well-mixed with a series of alliin solutions in the volume ratio 1:1, and the concentration variation of alliin was monitored in real time. The maximum reaction rates for various concentrations of the substrate were obtained using Origin 7.5 software. To this end, a double-reciprocal plot was used to calculate the parameters of the catalytic kinetics of the alliin/alliinase system, including the Michaelis constant ( K m ) and the maximum velocity ( V max ), which was compared to HPLC results.

In order to understand the sedimentary characteristics of shale and prospects of shale gas exploration in the lower Silurian Longmaxi Formation northern of Guizhou Province, outcrop and core observations, thin sections, X-ray diffraction analysis and other means are used. Studies show that the thickness of Longmaxi formation shale is generally large, range from 20m to 200m, mainly develop in the water shelf. The mineral mainly compose of detrital quartz and clay minerals, and five lithofacies can be identified: black shale, silty shale, carbonaceous shale, calcareous shale and argillaceous siltstone. Longmaxi shale kerogen type is mainlyIand II, and Ro value is average of 1.87%, mainly in the mature - over mature stage. In addition, the TOC content and gas content of Longmaxi shale is high, what’s more, these two parameters have good positive correlation. Compared with the Barnett shale, both of them have some similarities in the development environment shale, shale thickness and type of organic matter and TOC content, while, Longmaxi shale is deeply buried and post-destruction more intense. Longmaxi shale sedimentary environment is stable, high shale thickness and stable distribution, good quality source rocks and high brittle mineral content, which has meet the basic parameters of shale gas exploration and development, and has a large shale gas exploration potential in study area.

To investigate the association of LIG1 with the risk of lung cancer, all subjects of unrelated ethnic Han Chinese in Liaoning Province were involved in a hospital-based case-control study. The case group consisted of 370 histologically diagnosed lung cancer patients; 314 controls were selected from cancer-free patients during Dec. 2009 to Dec. 2011. LIG1 rs1050298SNP were analyzed by TaqMan real-time PCR method. All statistical analyses were performed with Statistical Product and Service Solution sv13.0 (SPSS). The genotype distribution frequency of LIG1 rs1050298 SNP displayed significant difference between the case and the control group. Individuals carrying the LIG1 rs1050298 T genotype had higher risks of lung cancer, especially those with squamous cell carcinoma.

The acquisition of the total organic carbon (TOC) content mainly relies on the geochemical analysis and logging data. Due to geochemical analysis is restricted by coring and experimental analysis, so it is difficult to get the continuous TOC data. Logging evaluation method for measuring TOC is very important for shale gas exploration. This paper presents a logging evaluation method that the shale is segmented according to sedimentary structures. Sedimentary structures were recognized by core, thin section and scanning electron microscope. Taking Wufeng-Longmaxi Formation, Silurian, Muai Syncline Belt, south of Sichuan Basin as research object, the shale is divided into three kinds: massive mudstone, unobvious laminated mudstone, and laminated mudstone. TOC within each mudstone are calculated using GR, resistivity and AC logging data, and an ideal result is achieved. This method is more efficient, faster and the vertical resolution is higher than △logR method.

The current card attendance check system in colleges has the problems of low efficiency and accuracy, and severe condition of taking attendance for others. To solve these problems, we have designed attendance check system for college students based on fingerprint identification technology. This system can share information with educational administration system, offering such functions as academic year and semester management, classroom management, lesson management, and schedule management, and attendance check authority management, etc. This system utilizes the characteristics of fingerprints, which carries uniqueness and stability and adopts the increasingly mature fingerprint identification technology. It can improve the quality and efficiency of attendance management for college students, and promote the information construction on campus.

For management the manhole cover effectively, the manhole covers intelligent monitoring terminal (MCIMT) based on dual frequency RFID and acceleration sensor was proposed in this paper. Through hardware designing, alarm algorithm designing, program debugging and packaging, we developed the MCIMT. The key performance testing and demonstration system operation shown the following results. Firstly, the MCIMT communication distance can achieve 800 meters under the open conditions and 300 meters under the occluded environment reliably. Secondly, the MCIMT embedded special state estimate algorithm can output the correct scene sate of manhole cover. Thirdly, the MCIMT could operation 3 years in manhole cover. Lastly, the demonstration system based on the MCIMT can alarm accurately and timely.