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497 results on '"HONDA, Yoichi"'

102. In situ detection and identification of hesperidin crystals in satsuma mandarin (Citrus unshiu) peel cells.

Author

60273489, 70252517, Inoue, Tsuyoshi, Yoshinaga, Arata, Takabe, Keiji, Yoshioka, Terutaka, Ogawa, Kazunori, Sakamoto, Masahiro, Azuma, Jun-ichi, Honda, Yoichi, 60273489, 70252517, Inoue, Tsuyoshi, Yoshinaga, Arata, Takabe, Keiji, Yoshioka, Terutaka, Ogawa, Kazunori, Sakamoto, Masahiro, Azuma, Jun-ichi, and Honda, Yoichi

Abstract

[Introduction]Hesperidin, a flavonoid known to have important pharmacological effects, accumulates particularly in the peels of satsuma mandarin (Citrus unshiu). Although histochemical studies have suggested that hesperidin forms crystals in some tissues of the Rutaceae and Umbelliferae, there has been no rigorous in situ detection or identification of hesperidin crystals in C. unshiu. [Objective]To characterise the chemical component of the crystals found in C. unshiu peels using Raman microscopy. [Methods]Sections of C. unshiu peels were made. The distribution and morphology of crystals in the sections were analysed microscopically. Raman microscopy was used to detect hesperidin in the sections directly. [Results]The crystals were more abundant in immature peel and were observed particularly in areas surrounding vascular bundles, around the border between the flavedo and albedo layers and just below the epidermal cells. In the morphological analysis by scanning electron microscopy, needle-shaped crystals aggregated and formed clusters of spherical crystals. Spectra obtained by Raman microscopy of the crystals in the peel sections were consistent with those of the hesperidin standard. [Conclusion]This study showed the detailed distribution of crystals in C. unshiu peels and their main component was identified using Raman microscopy to be hesperidin for the first time.

Published
2014

106. Identification of two mutations that cause defects in the ligninolytic system through an efficient forward genetics in the white-rot agaricomycete P leurotus ostreatus.

Author

Nakazawa, Takehito, Izuno, Ayako, Kodera, Rina, Miyazaki, Yasumasa, Sakamoto, Masahiro, Isagi, Yuji, and Honda, Yoichi

Subjects
PLEUROTUS ostreatus, CARBON cycle, LIGNIN peroxidases, MANGANESE peroxidase, MOLECULAR genetics, CHROMATIN
Abstract

White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs) and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, they successfully showed an efficient forward genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, they identified two genes - chd1 and wtr1 - mutations in which cause an almost complete loss of Mn2+-dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus. [ABSTRACT FROM AUTHOR]

Published
2017
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111. Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae

Author

Yao, Fang-Jie, Lu, Li-Xin, Wang, Peng, Fang, Ming, Zhang, You-Min, Chen, Ying, Zhang, Wei-Tong, Kong, Xiang-Hui, Lu, Jia, and Honda, Yoichi

Abstract

AbstractThe fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189–522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker “SCL-18” consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

Published
2018
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112. This title is unavailable for guests, please login to see more information.

Author

Yoshimura, Tsuyoshi, Takematsu, Yoko, Yamashita, Satoshi, Fujita, Motoko, Hattori, Takefumi, Honda, Yoichi, Ohmura, Wakako, Yanase, Yoshiyuki, Doi, Shuichi, 40230809, 70252517, 00303868, Yoshimura, Tsuyoshi, Takematsu, Yoko, Yamashita, Satoshi, Fujita, Motoko, Hattori, Takefumi, Honda, Yoichi, Ohmura, Wakako, Yanase, Yoshiyuki, Doi, Shuichi, 40230809, 70252517, and 00303868

Published
2012

135. Analysis of exposed cellulose surfaces in pretreated wood biomass using carbohydrate-binding module (CBM)–cyan fluorescent protein (CFP)

Author

Kawakubo, Takeshi, primary, Karita, Shuichi, additional, Araki, Yuko, additional, Watanabe, Shota, additional, Oyadomari, Masafumi, additional, Takada, Rie, additional, Tanaka, Fumio, additional, Abe, Kentaro, additional, Watanabe, Takahito, additional, Honda, Yoichi, additional, and Watanabe, Takashi, additional

Published
2010
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147. <Preliminary>Mn(III)-dependent Breakdown of 13(S)-Hydroperoxy-9Z, 11E-octadecadienoic Acid : A Key Free Radical Reaction in Lipid Peroxidation of Linoleic Acid by Manganese Peroxidase

Author

KATAYAMA, Shihoko, WATANABE, Takashi, ENOKI, Makiko, SATO, Shin, HONDA, Yoichi, and KUWAHARA, Masaaki

Subjects
acyl radical, lignin, Ceriporiopsis subvermispora, lipid peroxidation, manganese peroxidase, ESR
Abstract

この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。

Published
2000

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