1,179 results on '"Genetic regulation -- Analysis"'
Search Results
102. Regulation of hematopoietic niches by sympathetic innervation
- Author
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Aguila, Hector Leonardo
- Subjects
Genetic regulation -- Analysis ,Hematopoietic agents -- Analysis ,Biological sciences - Abstract
Study is presented which proposed that signals through the sympathetic nervous system are found to modify the activity of the hematopoietic niche, acting as regulators of the mobilization of hematopoietic progenitors. The results have added a new level of complexity to the cellular milieu responsible for generation and maintenance of the hematopoietic niche.
- Published
- 2006
103. Nitric oxide- and hydrogen peroxide-responsive gene regulation during cell death induction in tobacco (1)([W])
- Author
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Zago, Elisa, Morsa, Stijn, Dat, James F., Alard, Philippe, Ferrarini, Alberto, Inze, Dirk, Delledonne, Massimo, and Van Breusegem, Frank
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Cell death -- Research ,Genetic regulation -- Analysis ,Polymerase chain reaction -- Analysis ,Tobacco (Plant) -- Genetic aspects ,Tobacco (Plant) -- Growth ,Company growth ,Biological sciences ,Science and technology - Published
- 2006
104. Characterization of 43 non-protein-coding mRNA genes in Arabidopsis, including the MIR162a-derived transcripts (1)([W])
- Author
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Hirsch, Judith, Lefort, Vincent, Vankersschaver, Marion, Boualem, Adnane, Lucas, Antoine, Thermes, Claude, d'Aubenton-Carafa, Yves, and Crespi, Martin
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Arabidopsis thaliana -- Genetic aspects ,Arabidopsis thaliana -- Physiological aspects ,Genetic regulation -- Analysis ,Messenger RNA -- Genetic aspects ,Messenger RNA -- Research ,Biological sciences ,Science and technology - Published
- 2006
105. Cross-regulation of the Bacillus subtilis glnRA and tnrA genes provides evidence for DNA binding site discrimination by GlnR and TnrA
- Author
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Zalieckas, Jill M., Wray, Lewis V., Jr., and Fisher, Susan H.
- Subjects
Bacillus subtilis -- Genetic aspects ,Gene mutations -- Analysis ,Genetic regulation -- Analysis ,Genetic research ,Biological sciences - Abstract
Two Bacillus subtilis transcriptional factors, TnrA and GlnR, regulate gene expression in response to changes in nitrogen availability. These two proteins have similar amino acid sequences in their DNA binding domains and bind to DNA sites (GlnR/TnrA sites) that have the same consensus sequence. Expression of the mrA gene was found to be activated by TnrA and repressed by GlnR. Mutational analysis demonstrated that a GlnR/ TnrA site which lies immediately upstream of the -35 region of the tnrA promoter is required for regulation of tnrA expression by both GlnR and TnrA. Expression of the glnRA operon, which contains two GlnR/TnrA binding sites (glnRAo1 and glnRAo2) in its promoter region, is repressed by both GlnR and TnrA. The glnRAo2 site, which overlaps the -35 region of the glnRA promoter, was shown to be required for regulation by both GlnR and TnrA, while the glnRAol site which lies upstream of the -35 promoter region is only involved in GlnR-mediated regulation. Examination of TnrA binding to mrA and glnRA promoter DNA in gel mobility shift experiments showed that TnrA bound with an equilibrium dissociation binding constant of 55 nM to the GlnR/TnrA site in the tnrA promoter region, while the affinities of TnrA for the two GlnR/TnrA sites in the glnRA promoter region were greater than 3 [micro]M. These results demonstrate that GlnR and TnrA cross-regulate each other's expression and that there are differences in their DNA-binding specificities.
- Published
- 2006
106. Regulation and characterization of two nitroreductase genes, nprA and nprB, of Rhodobacter capsulatus
- Author
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Perez-Reinado, Eva, Blasco, Rafael, Castillo, Francisco, Moreno-Vivian, Conrado, and Roldan, M. Dolores
- Subjects
Genetic regulation -- Analysis ,Bacteria, Photosynthetic -- Genetic aspects ,Nitro compounds -- Chemical properties ,Biological sciences - Abstract
The identification, isolation and characterization of the nitroreductase genes, nprA and nprB, involved in 2,4-dinitrophenol reduction in the phototrophic bacterium Rhodobacter capsulatus B10 are described. The induction of the nprA gene by paraquat and by an oxidized redox status in the cell indicates that the SoxRS system can also be implicated in controlling nprA gene expression.
- Published
- 2005
107. Function and regulation of Alx4 in limb development: complex genetic interactions with Gli3 and Shh
- Author
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Kuijper, Sanne, Feitsma, Harma, Sheth, Rushikesh, Korving, Jeroen, Reijnen, Mark, and Meijlink, Frits
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Genetic regulation -- Analysis ,Homeobox genes -- Structure ,Homeobox genes -- Research ,Polydactyly -- Diagnosis ,Rats -- Research ,Rattus -- Research ,Biological sciences - Abstract
The role of the aristaless-related homeobox gene Alx4 in antero-postefior (AP-) patterning of the developing vertebrate limb has remained somewhat elusive. Polydactyly of Alx4 mutant mice is known to be accompanied by ectopic anterior expression of genes like Shh, Fgf4 and 5'Hoxd. We reported previously that polydactyly in Alx4 mutant mice requires SHH signaling, but we now show that in early [Alx4.sup.-/-] limb buds the anterior ectopic expression of Fgf4 and Hoxdl3, and therefore disruption of AP-patterning, occurs independently of SHH signaling. To better understand how Alx4 functions in the pathways that regulate AP-patterning, we also studied genomic regulatory sequences that are capable of directing expression of a reporter gene in a pattern corresponding to endogenous Alx4 expression in anterior limb bud mesenchyme. We observed, as expected for authentic Alx4 expression, expansion of reporter construct expression in a [Shh.sup.-/-] background. Total lack of reporter expression in a [Gli3.sup.-/-] background confirms the existence of Gli3-dependent and--independent Alx4 expression in the limb bud. Apparently, these two modules of Alx4 expression are linked to dissimilar functions. Keywords: Limb development; Alx4; Gli3; Antero-posterior patterning; Gene regulation; Sonic hedgehog; FGF4; Polydactyly
- Published
- 2005
108. Role of endogenous antisense RNA in cardiac gene regulation
- Author
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Luther, Hans Peter
- Subjects
Genetic regulation -- Research ,Genetic regulation -- Analysis ,Endorphins -- Research ,Endorphins -- Analysis ,RNA -- Research ,RNA -- Analysis ,Science and technology - Published
- 2005
109. Privacy, liberty, property, and the genetic modification of humans
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Moore, Adam D.
- Subjects
Genetically modified organisms -- Laws, regulations and rules ,Genetic regulation -- Analysis ,Privacy -- Laws, regulations and rules ,Privacy issue ,Government regulation ,Philosophy and religion - Abstract
Call one's genetic profile be owned? How about techniques to improve genetic profiles? A brave new world of genetic enhancement is upon us and we'd better be ready to answer such questions. Adam Moore is ready. He thinks the answers to these questions are resoundingly 'yes.' He defends this on the ground of individual liberty in the face of potential government control of such technology. He argues that genetic property is to be assimilated to rights of privacy and property in general and can be owned. He defends this against several important objections on the basis of societal overriding needs or goods.
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- 2005
110. Proteogenomic analysis of aneuploidy reveals divergent types of gene expression regulation across cellular pathways
- Subjects
Gene expression -- Analysis ,Genetic research -- Analysis ,Genes -- Analysis ,Genetic regulation -- Analysis ,Health - Abstract
2021 DEC 31 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- According to news reporting based on a preprint abstract, our journalists obtained the [...]
- Published
- 2021
111. The CepIR quorum-sensing system contributes to the virulence of Burkholderia cenocepacia respiratory infections
- Author
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Sokol, P.A., Sajjan, U., Visser, M.B., Gingues S., Forstner, J., and Kooi, C.
- Subjects
Bacteria -- Genetic aspects ,Bacteria -- Physiological aspects ,Biosynthesis -- Analysis ,Gene expression -- Physiological aspects ,Genetic regulation -- Analysis ,Genetic regulation -- Physiological aspects ,Genetic transcription -- Physiological aspects ,Lactones -- Genetic aspects ,Lactones -- Physiological aspects ,Microbiology -- Research ,Serine -- Genetic aspects ,Serine -- Physiological aspects ,Biological sciences - Abstract
The ceplR genes encode an N-acyl homoserine lactone (AHL)-dependent quorum-sensing system consisting of an AHL synthase that directs the synthesis of N-octanoyI-L-homoserine lactone (OHL) and N-hexanoyI-L-homoserine lactone and a transcriptional regulator. The virulence of cepIR mutants was examined in two animal models. Rats were infected with agar beads containing Burkholderia cenocepacia K56-2, K56-12 (cepl: :[Tp.sup.r]) or K56-R2 (cepR'' Tn5-OT182). At 10 days post-infection, the extent of lung histopathological changes was significantly lower in lungs infected with K56-12 or K56-R2 compared to the parent strain. Intranasal infections were performed in [Cftr.sup.(-/-)] mice and their wild-type siblings. K56-2 was more virulent in both groups of mice. K56-12 was the least virulent strain and was not invasive in the [Cftr.sup.(-/-)] mice. OHL was readily detected in lung homogenates from [Cftr.sup.(-/-)] mice infected with K56-2 but was only detected at levels slightly above background in a few mice infected with K56-12. Lung homogenates from mice infected with K56-2 had significantly higher levels of the inflammatory mediators murine macrophage inflammatory protein-2, KC/N51, interleukin-1 [beta] and interleukin-6 than those from K56-12-infected animals. These studies indicate that a functional CepIR quorum-sensing system contributes to the severity of B. cenocepacia infections. A zinc metalloprotease gene (zmpA) was shown to be regulated by CepR and may be one of the factors that accounts for the difference in virulence between the cepl mutant and the parent strain.
- Published
- 2003
112. In vivo effect of mutations at the PRPP binding site of the Bacillus subtilis purine repressor
- Author
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Rappu, Pekka, Pullinen, Terhi, and Mantsala, Pekka
- Subjects
Gene mutations -- Influence ,Gene mutations -- Physiological aspects ,Nucleotides -- Physiological aspects ,Genetic regulation -- Analysis ,DNA-ligand interactions -- Analysis ,Biological sciences - Abstract
The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR.
- Published
- 2003
113. MgrA, an orthologue of mga, acts as a transcriptional repressor of the genes within the rlrA pathogenicity islet in Streptococcus pneumoniae
- Author
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Hemsley, Carolyn, Joyce, Elizabeth, Hava, David L., Kawale, Amita, and Camilli, Andrew
- Subjects
Genetic regulation -- Analysis ,Genetic transcription -- Analysis ,Virulence (Microbiology) -- Genetic aspects ,Streptococcus pneumoniae -- Genetic aspects ,Biological sciences - Abstract
Streptococcus pneumoniae normally resides in the human nasopharynx in a nondisease state. In response to unknown triggers this organism can descend to the lower respiratory tract and/or invade the bloodstream. Regulation and activation of virulence genes play essential roles in this process of disease development. Characterization of S. pneumoniae regulatory networks has been a recent area of interest, but despite inroads little is known about regulation of virulence genes in this pathogen. A putative transcriptional regulator in S. pneumoniae, mgrA, which exhibits homology to the virulence gene activator mga of group A streptococcus, was previously identified as a regulator that is required for development of pneumonia in a murine model. In this study we confirmed that mgrA plays a role in both nasopharyngeal carriage and pneumonia. Transcriptional profiling by microarray technology was used to show that mgrA acts as a repressor of the previously characterized rlrA pathogenicity islet. This is manifested phenotypically by a decrease in adherence to epithelial cells in tissue culture since the rlrA pathogenicity islet contains genes mediating adherence.
- Published
- 2003
114. SoxRS-regulated expression and genetic analysis of the yggX gene of Escherichia coli
- Author
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Pomposiello, Pablo J., Koutsolioutsou, Anastasia, Carrasco, Daniel, and Demple, Bruce
- Subjects
Protein metabolism -- Physiological aspects ,Escherichia coli -- Research ,Oxidative stress -- Genetic aspects ,Genetic regulation -- Analysis ,Biological sciences - Abstract
Genomic studies with bacteria have identified redox-responsive genes without known roles in counteracting oxidative damage. Previous transcriptional profiling showed that expression of one such gene, yggX, was activated by superoxide stress in Escherichia coli. Here we show that this activation could be mimicked by artificial expression of the regulatory protein SoxS. Northern analysis confirmed the transcriptional activation of yggX by oxidative stress or SoxS expression but not in response to the related MarA or Rob proteins. Northern analysis showed that mltC, which codes for a peptidoglycan hydrolase and is positioned immediately downstream of yggX, was also regulated by oxidative stress or ectopic expression of SoxS. Purified SoxS protein bound to the predicted yggX promoter region, between positions 223 and 163 upstream from the yggX translational start site. Within this region, a 20-bp sequence was found to be necessary for oxidative stress-mediated activation of yggX transcription. A yggX deletion strain was hypersensitive to the redox-cycling agent paraquat, and a plasmid expressing YggX complemented the sensitivity of the deletion strain. Under exposure to paraquat, the yggX deletion strain showed a deficiency in aconitase activity compared to the isogenic wild-type strain, while expression of YggX from a multicopy plasmid increased the aconitase levels above those of the wild-type strain. These results demonstrate the direct regulation of the yggX gene by the redox-sensing SoxRS system and provide further evidence for the involvement of yggX in protection of iron-sulfur proteins against oxidative damage.
- Published
- 2003
115. CTP limitation increases expression of CTP synthase in Lactococcus lactis
- Author
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Jorgensen, Casper Moller, Hammer, Karin, and Martinussen, Jan
- Subjects
Enzymes -- Synthesis ,Bacillus subtilis -- Research ,Genetic regulation -- Analysis ,Gene expression -- Analysis ,Biological sciences - Abstract
CTP synthase is encoded by the pyrG gene and catalyzes the conversion of UTP to CTP. A Lactococcus lactis pyrG mutant with a cytidine requirement was constructed, in which [beta]-galactosidase activity in a pyrG-lacLM transcriptional fusion was used to monitor gene expression of pyrG. A 10-fold decrease in the CTP pool induced by cytidine limitation was found to immediately increase expression of the L. lactis pyrG gene. The final level of expression of pyrG is 37-fold higher than the uninduced level. CTP limitation has pronounced effects on central cellular metabolism, and both RNA and protein syntheses are inhibited. Expression of pyrG responds only to the cellular level of CTP, since expression of pyrG has no correlation to alterations in UTP, GTP, and ATP pool sizes. In the untranslated pyrG leader sequence a potential terminator structure can be identified, and this structure is required for regulation of the pyrG gene. It is possible to fold the pyrG leader in an alternative structure that would prevent the formation of the terminator. We suggest a model for pyrG regulation in L. lactis, and probably in other gram-positive bacteria as well, in which pyrG expression is directly dependent on the CTP concentration through an attenuator mechanism. At normal CTP concentrations a terminator is preferentially formed in the pyrG leader, thereby reducing expression of CTP synthase. At low CTP concentrations the RNA polymerase pauses at a stretch of C residues in the pyrG leader, thereby allowing an antiterminator to form and transcription to proceed. This model therefore does not include any trans-acting protein for sensing the CTP concentration as previously proposed for Bacillus subtilis.
- Published
- 2003
116. Identification and characterization of a pSLA2 plasmid locus required for linear DNA replication and circular plasmid stable inheritance in Streptomyces lividans
- Author
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Qin, Zhongjun, Shen, Meijuan, and Cohen, Stanley N.
- Subjects
Streptomyces -- Genetic aspects ,Genetic regulation -- Analysis ,Plasmids -- Genetic aspects ,DNA replication -- Genetic aspects ,Biological sciences - Abstract
Streptomyces linear plasmids and linear chromosomes can replicate also in a circular form when their telomeres are deleted. The 17-kb linear plasmid pSLA2 has been a useful model in studies of such replicons. Here we report that the minimal origin initiating replication of pSLA2-derived plasmids as circular molecules cannot propagate these plasmids in a linear mode unless they also contain a novel plasmid-encoded locus, here interferes with the establishment of pSLA2 in circular form in Streptomyces lividans transformants. The additional presence of an adjacent divergently transcribed locus, rorA (rlrA override), which strongly resembles the kor (kil override) transcription control genes identified previously on Streptomyces plasmids, reversed the detrimental effects of rlrA on plasmid establishment and additionally stabilized circular plasmid inheritance by spores during the S. lividans life cycle. While the effects of the rlrA/rorA locus of pSLA2 were seen also on linear plasmids derived from the unrelated SLP2 replicon, they did not extend to plasmids whose replication was initiated at a cloned chromosomal origin. Our results establish the existence of, and provide the initial description of, a novel plasmid-borne regulatory system that differentially affects the propagation of linear and circular plasmids in Streptomyces.
- Published
- 2003
117. A transcriptional pause synchronizes translation with transcription in the tryptophanase operon leader region
- Author
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Gong, Feng and Yanofsky, Charles
- Subjects
Operons -- Genetic aspects ,Protein biosynthesis -- Genetic aspects ,Genetic regulation -- Analysis ,Genetic transcription -- Analysis ,Biological sciences - Abstract
Regulation of transcription of the tryptophanase operon requires that translation of its leader peptide coding region, tnaC, be coupled with its transcription. We show in vitro that a transcription pause site exists at the end of the tnaC coding region and that translation of tnaC releases the paused transcription complex, coupling transcription with translation.
- Published
- 2003
118. Molecular characterization of the [Mg.sup.2+]-responsive PhoP-PhoQ regulon in Salmonella enterica
- Author
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Lejona, Sergio, Aguirre, Andres, Cabeza, Maria Laura, Vescovi, Eleonora Garcia, and Soncini, Fernando C.
- Subjects
Genetic regulation -- Analysis ,Gene expression -- Analysis ,Salmonella -- Genetic aspects ,Biological sciences - Abstract
The PhoP/PhoQ two-component system controls the extracellular magnesium deprivation response in Salmonella enterica. In addition, several virulence-associated genes that are mainly required for intramacrophage survival during the infection process are under the control of its transcriptional regulation. Despite shared [Mg.sup.2+] modulation of the expression of the PhoP-activated genes, no consensus sequence common to all of them could be detected in their promoter regions. We have investigated the transcriptional regulation and the interaction of the response regulator PhoP with the promoter regions of the PhoP-activated loci phoPQ, mgtA, slyB, pmrD, pcgL, phoN, pagC, and mgtCB. A direct repeat of the heptanucleotide sequence (G/T)GTTTA(A/T) was identified as the conserved motif recognized by PhoP to directly control the gene expression of the first five loci, among which the first four are ancestral to enterobacteria. On the other hand, no direct interaction of the response regulator with the promoter of phoN, pagC, or mgtCB was apparent by either in vitro or in vivo assays. These loci are Salmonella specific and were probably acquired by horizontal DNA transfer. Besides, sequence analysis of pag promoters revealed the presence of a conserved PhoP box in 6 out of the 12 genes analyzed. Our results strongly suggest that the expression of a set of [Mg.sup.2+]-controlled genes is driven by PhoP via unknown intermediate regulatory mechanisms that could also involve ancillary factors.
- Published
- 2003
119. Molecular architecture of the regulatory Locus sae of Staphylococcus aureus and its impact on expression of virulence factors
- Author
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Steinhuber, Andrea, Goerke, Christiane, Bayer, Manfred G., Doring, Gerd, and Wolz, Christiane
- Subjects
Staphylococcus aureus -- Genetic aspects ,Genetic regulation -- Analysis ,Operons -- Genetic aspects ,Virulence (Microbiology) -- Genetic aspects ,Gene expression -- Analysis ,Biological sciences - Abstract
We characterized the sac operon, a global regulator for virulence gene expression in Staphylococcus aureus. A Tn917 sae mutant was obtained by screening a Tn917 library of the agr mutant ISP479Mu for clones with altered hemolytic activity. Sequence analysis of the sac operon revealed two additional open reading frames (ORFs) (ORF3 and ORF4) upstream of the two-component regulatory genes saeR and saeS. Four overlapping sae-specific transcripts (T1 to T4) were detected by Northern blot analysis, and the transcriptional initiation points were mapped by primer extension analysis. The T1, T2, and T3 mRNAs are probably terminated at the same stem-loop sequence downstream of saeS. The T1 message (3.1 kb) initiates upstream of ORF4, T2 (2.4 kb) initiates upstream of ORF3, and T3 (2.0 kb) initiates in front of saeR. T4 (0.7 kb) represents a monocistronic mRNA encompassing ORF4 only. sae-specific transcripts were detectable in all of the 40 different clinical S. aureus isolates investigated. Transcript levels were at maximum during the post-exponential growth phase. The sae mutant showed a significantly reduced rate of invasion of human endothelial cells, consistent with diminished transcription and expression of fnbA. The expression of type 5 capsular polysaccharide is activated in the sae mutant of strain Newman, as shown by immunofluorescence and promoter-reporter fusion experiments. In summary, the sae operon constitutes a four-component regulator system which acts on virulence gene expression in S. aureus.
- Published
- 2003
120. Regulation of expression of cellulosomal cellulase and hemicellulase genes in Clostridium cellulovorans
- Author
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Han, Sung Ok, Yukawa, Hideaki, Inui, Masayuki, and Doi, Roy H.
- Subjects
Protein biosynthesis -- Analysis ,Genetic regulation -- Analysis ,Gene expression -- Analysis ,Biological sciences - Abstract
The regulation of expression of the genes encoding the cellulases and hemicellulases of Clostridium cellulovorans was studied at the mRNA level with cells grown under various culture conditions. A basic pattern of gene expression and of relative expression levels was obtained from cells grown in media containing poly-, di- or monomeric sugars. The cellulase (cbpA and engE) and hemicellulase (xynA) genes were coordinately expressed in medium containing cellobiose or cellulose. Growth in the presence of cellulose, xylan, and pectin gave rise to abundant expression of most genes (cbpA-exgS, engH, hbpA, manA, engM, engE, xynA, and/or pelA) studied. Moderate expression of cbpA, engH, manA, engE, and xynA was observed when cellobiose or fructose was used as the carbon source. Low levels of mRNA from cbpA, manA, engE, and xynA were observed with cells grown in lactose, mannose, and locust bean gum, and very little or no expression of cbpA, engH, manA, engE, and xynA was detected in glucose-, galactose-, maltose-, and sucrose-grown cells. The cbpA-exgS and engE genes were most frequently expressed under all conditions studied, whereas expression of xynA and pelA was more specifically induced at higher levels in xylan- or pectin-containing medium, respectively. Expression of the genes (cbpA, hbpA, manA, engM, and engE) was nat observed in the presence of most soluble di- or monosaccharides such as glucose. These results support the hypotheses that there is coordinate expression of some cellulases and hemicellulases, that a catabolite repression type of mechanism regulates cellulase expression in rapidly growing cells, and that the presence of hemicelluloses has an effect on cellulose utilization by the cell.
- Published
- 2003
121. Global mutational analysis of NtrC-like activators in Myxococcus xanthus: identifying activator mutants defective for motility and fruiting body development
- Author
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Caberoy, Nora B., Welch, Roy D., Jakobsen, Jimmy S., Slater, Steven C., and Garza, Anthony G.
- Subjects
Bacterial genetics -- Research ,Gene mutations -- Analysis ,Genetic regulation -- Analysis ,Myxobacterales -- Genetic aspects ,Biological sciences - Abstract
The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found that eight of these insertions cause developmental defects. Hence, these results are consistent with the idea that M. xanthus uses a series of different NtrC-like activators during fruiting body development. Four of the eight developmental mutants we identified have motility defects. The nlal, nla19, and nla23 mutants show S-motility defects, while the nla24 mutant shows defects in both S-motility and A-motility. During development, aggregation of the nlal, nla19, and nla23 mutants is delayed slightly and the nla24 mutant shows no signs of aggregation or sporulation. The nla4, nla6, nla18, and nla28 mutants have no appreciable loss in motility, but they fail to aggregate and to sporulate normally. The nla18 mutant belongs to a special class of developmental mutants whose defects can be rescued when they are codeveloped with wild-type cells, suggesting that nla18 fails to produce a cell-cell signal required for development. The three remaining activator mutants, nla4, nla6, and nla28, appear to have complex developmental phenotypes that include deficiencies in cell-cell developmental signals.
- Published
- 2003
122. Constitutive activation of the Escherichia coli Pho regulon upregulates rpoS translation in an Hfq-dependent fashion
- Author
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Ruiz, Natividad and Silhavy, Thomas J.
- Subjects
Escherichia coli -- Research ,Metabolic regulation -- Genetic aspects ,RNA -- Physiological aspects ,Genetic translation -- Genetic aspects ,Genetic regulation -- Analysis ,Biological sciences - Abstract
Regulation of the [sigma] factor RpoS occurs at the levels of transcription, translation, and protein stability activity, and it determines whether Escherichia coli turns on or off the stationary-phase response. To better understand the regulation of RpoS, we conducted genetic screens and found that mutations in the pst locus cause accumulation of RpoS during exponential growth. The pst locus encodes for the components of the high-affinity transport system for inorganic phosphate ([P.sub.i]), which is involved in sensing [P.sub.i] levels in the environment. When the Pst transporter is compromised (either by mutation or by [P.sub.i] starvation), the two-component system PhoBR activates the transcription of the Pho regulon, a subset of genes that encode proteins for transporting and metabolizing alternative phosphate sources. Our data show that strains carrying mutations which constitutively activate the Pho regulon have increased rpoS translation during exponential growth. This upregulation of rpoS translation is Hfq dependent, suggesting the involvement of a small regulatory RNA (sRNA). The transcription of this yet-to-be-identified sRNA is regulated by the PhoBR two-component system.
- Published
- 2003
123. Redefining the role of psr in [beta]-lactam resistance and cell autolysis of Enterococcus hirae
- Author
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Sapunaric, Frederic, Franssen, Christine, Stefanic, Patrick, Amoroso, Ana, Dardenne, Olivier, and Coyette, Jacques
- Subjects
Cellular control mechanisms -- Genetic aspects ,Genetic regulation -- Analysis ,Gene expression -- Analysis ,Penicillin -- Physiological aspects ,Biological sciences - Abstract
The contribution of penicillin-binding protein 5 (PBP5) and the PBP5 synthesis repressor (Psr) to the [beta]-lactam resistance, growth, and cell autolysis of wild-type strain ATCC 9790 and resistant strain R40 of Enterococcus hirae was investigated by disruption or substitution of the corresponding pbp5 and psr genes by Campbell-type recombination. The resulting modifications were confirmed by hybridization and PCR. The low susceptibility of E. hirae to [beta]-lactams was confirmed to be largely dependent on the presence of PBP5. However, against all expectations, inactivation of psr in ATCC 9790 or complementation of R40 cells with psr did not modify the susceptibility to benzylpenicillin or the growth and cell autolysis rates. These results indicated that the psr gene does not seem to be involved in the regulation of PBP5 synthesis and consequently in [beta]-lactam resistance or in the regulation of cell autolysis in E. hirae.
- Published
- 2003
124. Characterization and regulation of the genes for a novel anthranilate 1,2-dioxygenase from Burkholderia cepacia DBO1
- Author
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Chang, Hung-Kuang, Mohseni, Paria, and Zylstra, Gerben J.
- Subjects
Enzymes -- Synthesis ,Microbial enzymes -- Genetic aspects ,Gene expression -- Analysis ,Genetic regulation -- Analysis ,Biological sciences - Abstract
Anthranilate (2-aminobenzoate) is an important intermediate in tryptophan metabolism. In order to investigate the degradation of tryptophan through anthranilate by Burkholderia cepacia, several plasposon mutations were constructed of strain DBO1 and one mutant with the plasposon insertion in the anthranilate dioxygenase (AntDO) genes was chosen for further study. The gene sequence obtained from flanking DNA of the plasposon insertion site revealed unexpected information. B. cepacia DBO1 AntDO (designated AntDO-3C) is a three-component Rieske-type [2Fe-2S] dioxygenase composed of a reductase (AndAa), a ferredoxin (AndAb), and a two-subunit oxygenase (AndAcAd). This is in contrast to the two-component (an oxygenase and a reductase) AntDO enzyme from Acinetobacter sp. strain ADP1, P. aeruginosa PAO1, and P. putida P111. AntDO from strains ADP1, PAO1, and P111 are closely related to benzoate dioxygenase, while AntDO-3C is closely related to aromatic hydrocarbon dioxygenases from Novosphingobium aromaticivorans F199 and Sphingomonas yanoikuyae B1 and 2-chlorobenzoate dioxygenase from P. aeruginosa strains 142 and JB2. Escherichia coli cells expressing the functional AntDO-3C genes transform anthranilate and salicylate (but not 2-chlorobenzoate) to catechol. The enzyme includes a novel reductase whose absence results in less efficient transformation of anthranilate by the oxygenase and ferredoxin. AndR, a possible AraC/XyIS-type transcriptional regulator, was shown to positively regulate expression of the andAcAdAbAa genes. Anthranilate was the only effector (of 12 aromatic compounds tested) that was able to induce expression of the genes.
- Published
- 2003
125. The naphthalene catabolic (nag) genes of Ralstonia sp. strain U2 are an operon that is regulated by NagR, a LysR-type transcriptional regulator
- Author
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Jones, Rheinallt M., Britt-Compton, Bethan, and Williams, Peter A.
- Subjects
Microbial enzymes -- Research ,Operons -- Genetic aspects ,Genetic regulation -- Analysis ,Gene expression -- Genetic aspects ,Biological sciences - Abstract
In Ralstonia sp. strain U2, the nag catabolic genes, which encode the enzymes for the pathway that catabolizes naphthalene via the alternative ring cleavage gentisate pathway, are transcribed as an operon under the same promoter. nagR, which encodes a LysR-type transcriptional regulator, is divergently transcribed compared to the nag catabolic genes. A 4-bp frameshift deletion in nagR demonstrated that NagR is required for expression of the nag operon. The transcriptional start of the nag operon was mapped, and a putative -10, -35 [[sigma].sup.70]-type promoter binding site was identified. Further upstream, a site proximal to the promoter was identified as a site that has bases which have been found to be conserved in the activator-binding motif of other naphthalene pathways. Transcriptional fusion studies demonstrated that NagR regulates the expression of the nag operon positively in the presence of salicylate and to a lesser extent in the presence of 2-nitrobenzoate. Mutation of the LysR-type activator-binding motif in the nag promoter-proximal region resulted in a loss of inducibility of a lacZ reporter gene transcriptionally fused to nagAa, the first gene of the operon. However, other mutations in the region increased the effectiveness of salicylate as an inducer.
- Published
- 2003
126. Dual overlapping promoters control napF (periplasmic nitrate reductase) operon expression in Escherichia coli K-12
- Author
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Stewart, Valley, Bledsoe, Peggy J., and Williams, Stanly B.
- Subjects
Promoters (Genetics) -- Genetic aspects ,Operons -- Genetic aspects ,Genetic regulation -- Analysis ,Gene expression -- Genetic aspects ,Biological sciences - Abstract
Escherichia coli elaborates a flexible respiratory metabolism, involving differential synthesis of isoenzymes for many oxidation and reduction reactions. Periplasmic nitrate reductase, encoded by the napFDAGHBC operon, functions with concentrations of nitrate that are too low to support respiration by membrane-bound nitrate reductase. The napF operon control region exhibits unusual organization of DNA binding sites for the transcription regulators Fnr and NarP, which activate transcription in response to anaerobiosis and nitrate, respectively. Previous studies have shown that the napF operon control region directs synthesis of two transcripts whose 5' ends differ by about 3 nucleotides. We constructed mutant control regions in which either of the two promoter--10 regions is inactivated. Results indicate that the downstream promoter (P1) was responsible for Fnr- and NarP-regulated napF operon expression, whereas transcription from the upstream promoter (P2) was activated only weakly by the Fnr protein and was inhibited by phospho-NarP and -NarL proteins. The physiological function of promoter P2 is unknown. These results establish the unconventional napF operon control region architecture, in which the major promoter P1 is activated by the Fnr protein bound to a site centered at -64.5 with respect to the transcription initiation site, working in conjunction with the phospho-NarP protein bound to a site centered at -44.5.
- Published
- 2003
127. PI3P signaling regulates receptor sorting but not transport in the endosomal pathway
- Author
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Petiot, A., Faure, J., Stenmark, H., and Gruenberg, J.
- Subjects
Growth factors -- Genetic aspects ,Phosphoinositides -- Physiological aspects ,Phosphoinositides -- Genetic aspects ,Hormones -- Genetic aspects ,Genetic regulation -- Analysis ,Cell research -- Analysis ,Biological sciences - Abstract
While evidence is accumulating that phosphoinositide signaling plays a crucial role in growth factor and hormone receptor down-regulation, this signaling pathway has also been proposed to regulate endosomal membrane transport and multivesicular endosome biogenesis. Here, we have followed the fate of the down-regulated EGF receptor (EGFR) and bulk transport (fluid phase) markers in the endosomal pathway in vivo and in vitro. We find that bulk transport from early to late endosomes is not affected after inhibition of the phosphatidylinositol-3-phosphate (PI3P) signaling pathway, but that the EGFR then remains trapped in early endosomes. Similarly, we find that hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is not directly involved in bulk solute transport, but is required for EGFR sorting. These observations thus show that transport and sorting can be uncoupled in the endosomal pathway. They also show that PI3P signaling does not regulate the core machinery of endosome biogenesis and transport, but controls the sorting of down-regulated receptor molecules in early endosomes via Hrs.
- Published
- 2003
128. Identification of genes that regulate a left-right asymmetric neuronal migration in Caenorhabditis elegans
- Author
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Ch'ng, QueeLim, Williams, Lisa, Lie, Yung S., Sym, Mary, Whangbo, Jennifer, and Kenyon, Cynthia
- Subjects
Gene mutations -- Physiological aspects ,Cell migration -- Genetic aspects ,Gene expression -- Physiological aspects ,Genetic regulation -- Analysis ,Cells -- Genetic aspects ,Genetics -- Research ,Biological sciences - Abstract
In C. elegans, cells of the QL and QR neuroblast lineages migrate with left-right asymmetry; QL and its descendants migrate posteriorly whereas QR and its descendants migrate anteriorly. One key step in generating this asymmetry is the expression of the Hox gene mab-5 in the QL descendants but not in the QR descendants. This asymmetry appears to be coupled to the asymmetric polarizations and movements of QL and QR as they migrate and relies on an asymmetric response to an EGL-20/Wnt signal. To identify genes involved in these complex layers of regulation and to isolate targets of mab-5 that direct posterior migrations, we screened visually for mutants with cell migration defects in the QL and QR lineages. Here, we describe a set of new mutants (qid-5, qid-6, qid-7, and qid-8) that primarily disrupt the migrations of the QL descendants. Most of these mutants were defective in mab-5 expression in the QL lineage and might identify genes that interact directly or indirectly with the EGL-20/Wnt signaling pathway.
- Published
- 2003
129. Identification of Trans-dominant modifiers of Prat expression in Drosophila melanogaster
- Author
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Malmanche, Nicolas and Clark, Denise V.
- Subjects
Genetic regulation -- Analysis ,Gene expression -- Physiological aspects ,Genetic transcription -- Physiological aspects ,Drosophila -- Genetic aspects ,Genetics -- Research ,Biological sciences - Abstract
The first committed step in the purine de novo synthesis pathway is performed by amidophosphoribosyl-transferase (EC 2.4.2.14) or Prat. Drosophila melanogaster Prat is an essential gene with a promoter that lacks a TATA-box and initiator element and has multiple transcription start sites with a predominant start site. To study the regulation of Prat expression in the adult eye, we used the Prat:bw reporter gene, in which the Prat coding region was replaced with the brown (bw) coding region. The pale-orange eye color of a single copy of Prat:bw prompted us to use a multicopy array of Prat:bw that was derived using P transposase mutagenesis and produces a darker-orange eye color in a [bw.sup.D]; st genetic background. We used a 13-copy array of Prat:bw as a tool to recover dominant EMS-induced mutations that affect the expression of the transgene. After screening 21,000 [F.sub.1]s for deviation from the orange eye color, we isolated 23 dominant modifiers: 21 suppressors (1 Y-linked, 5 X-linked, 4 2-linked, and 11 3-linked) and 2 enhancers (1 2-linked and 1 3-linked). Quantification of their effect on endogenous Prat gene expression, using RT-PCR in young adult fly heads, identifies a subset of modifiers that are candidates for genes involved in regulating Prat expression.
- Published
- 2003
130. Mutation in the relA gene of Vibrio cholerae affects in vitro and in vivo expression of virulence factors
- Author
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Haralalka, Shruti, Nandi, Suvobroto, and Bhadra, Rupak K.
- Subjects
Amino acids -- Physiological aspects ,Bacterial proteins -- Genetic aspects ,Virulence (Microbiology) -- Genetic aspects ,Gene expression -- Physiological aspects ,Genetic regulation -- Analysis ,Escherichia coli -- Genetic aspects ,Gene mutations -- Physiological aspects ,Bacteriology -- Research ,Biological sciences - Abstract
The relA gene product determines the level of (p)ppGpp, the effector nucleotides of the bacterial stringent response that are also involved in the regulation of other functions, like antibiotic production and quorum sensing. In order to explore the possible involvement of relA in the regulation of virulence of Vibrio cholerae, a relA homolog from the organism (rel[A.sub.VCH]) was cloned and sequenced. The rel[A.sub.VCH gene encodes a 738-aminoacid protein having functions similar to those of other gram-negative bacteria, including Escherichia coli. A [DELTA]relA::kan allele was generated by replacing ~31% of the open reading frame of wild-type relA of V. cholerae El Tor strain C6709 with a kanamycin resistance gene. The V. cholerae relA mutant strain thus generated, SHK17, failed to accumulate (p)ppGpp upon amino acid deprivation. Interestingly, compared to the wild type, C6709, the mutant strain SHK17 exhibited significantly reduced in vitro production of two principal virulence factors, cholera toxin (CT) and toxin-coregulated pilus (TCP), under virulence gene-inducing conditions. In vivo experiments carried out in rabbit ileal loop and suckling mouse models also confirmed our in vitro results. The data suggest that (p)ppGpp is essential for maximal expression of CT and TCP during in vitro growth, as well as during intestinal infection by virulent V. cholerae. Northern blot and reverse transcriptase PCR analyses indicated significant reduction in the transcript levels of both virulence factors in the relA mutant strain SHK17. Such marked alteration of virulence phenotypes in SHK17 appears most likely to be due to down regulation of transcript levels of toxR and toxT, the two most important virulence regulatory genes of V. cholerae. In SHKI7, the altered expression of the two outer membrane porin proteins, OmpU and OmpT, indicated that the relA mutation most likely affects the ToxR-dependent virulence regulatory pathway, because it had been shown earlier that ToxR directly regulates their expression independently of ToxT.
- Published
- 2003
131. Microarray transcription analysis of clinical Staphylococcus aureus isolates resistant to Vancomycin
- Author
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Mongodin, Emmanuel, Finan, Jon, Climo, Michael W., Rosato, Adriana, Gill, Steven, and Archer Gordon L.
- Subjects
Genetic regulation -- Analysis ,Plant cell walls -- Genetic aspects ,Biosynthesis -- Analysis ,Genetic transcription -- Physiological aspects ,DNA microarrays -- Genetic aspects ,Vancomycin -- Physiological aspects ,Staphylococcus aureus -- Genetic aspects ,Bacteriology -- Research ,Biological sciences - Abstract
The transcriptomes of vancomycin intermediate-resistance Staphylococcus aureus (VISA) clinical isolates HIP5827 and Mu50 (MIC = 8 [micro]g/ml) were compared to those of highly vancomycin-resistant S. aureus (VRSA; MIC = 32 [micro]/ml) passage derivatives by microarray. There were 35 genes with increased transcription and 16 genes with decreased transcription in common between the two VRSAs compared to those of their VISA parents. Of the 35 genes with increased transcription, 15 involved purine biosynthesis or transport, and the regulator (purR) of the major purine biosynthetic operon (purE-purD) was mutant. We hypothesize that increased energy (ATP) is required to generate the thicker cell walls that characterize resistant mutants.
- Published
- 2003
132. Unusual integrase gene expression on the clc genomic island in Pseudomonas sp. strain B13
- Author
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Sentchilo, V., Ravatn, R., Werlen, C., Zehnder, A.J.B., and van der Meer J.R.
- Subjects
Cells -- Genetic aspects ,Genetic regulation -- Analysis ,Pseudomonas -- Genetic aspects ,Chromosomes -- Genetic aspects ,Gene expression -- Physiological aspects ,Biological sciences - Abstract
An unusual type of gene expression from an integrase promoter was found in cultures of the bacterium Pseudomonas sp. strain B13. The promoter controls expression of the intB13 integrase gene, which is present near the right end of a 105-kb conjugative genomic island (the clc element) encoding catabolism of aromatic compounds. The enzymatic activity of integrase IntB13 is essential for site-specific integration of the clc element into the bacterial host's chromosome. By creating transcription fusions between the intB13 promoter and the gfp gene, we showed that integrase expression in strain B13 was inducible under stationary-phase conditions but, strangely, occurred in only a small proportion of individual bacterial cells rather than equally in the whole population. Integrase expression was significantly stimulated by growing cultures on 3-chlorobenzoate. High cell density, heat shock, osmotic shock, UV irradiation, and treatment with alcohol did not result in measurable integrase expression. The occurrence of the excised form of the clc element and an increase in the rates of clc element transfer in conjugation experiments correlated with the observed induction of the intB13'-gfp fusion in stationary phase and in the presence of 3-chlorobenzoate. This suggested that activation of the intB13 promoter is the first step in stimulation of clc transfer. To our knowledge, this is the first report of a chlorinated compound's stimulating horizontal transfer of the genes encoding its very metabolism.
- Published
- 2003
133. The phosphate starvation stimulon of Corynebacterium glutamicum determined by DNA microarray analyses
- Author
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Ishige, Takeru, Krause, Malgorzata, Bott, Michael, Wendisch, Volker F., and Sahm, Hermann
- Subjects
Genetic regulation -- Analysis ,Bacteria -- Genetic aspects ,Bacteria -- Growth ,RNA -- Genetic aspects ,Gene expression -- Physiological aspects ,DNA microarrays -- Genetic aspects ,Phosphates -- Physiological aspects ,Bacteriology -- Research ,Company growth ,Biological sciences - Abstract
The phosphate ([P.sub.i]) starvation stimulon of Corynebacterium glutamicum was characterized by global gene expression analysis by using DNA microarrays. Hierarchical cluster analysis of the genes showing altered expression 10 to 180 min after a shift from [P.sub.i]-sufficient to [P.sub.i]-limiting conditions led to identification of five groups comprising 92 genes. Four of these groups included genes which are not directly involved in P metabolism and changed expression presumably due to the reduced growth rate observed after the shift or to the exchange of medium. One group, however, comprised 25 genes, most of which are obviously related to phosphorus (P) uptake and metabolism and exhibited 4- to >30-fold-greater expression after the shift to [P.sub.i] limitation. Among these genes, the RNA levels of the pstSCAB (ABC-type [P.sub.i] uptake system), glpQ (glycero-phosphoryldiester phosphodiesterase), ugpAEBC (ABC-type sn-glycerol 3-phosphate uptake system), phoH (unknown function), nucH (extracellular nuclease), and Cgl0328 (5'-nucleotidase or related esterase) genes were increased, and pstSCAB exhibited a faster response than the other genes. Transcriptional fusion analyses revealed that elevated expression of pstSCAB and ugpAEBC was primarily due to transcriptional regulation. Several genes also involved in P uptake and metabolism were not affected by [P.sub.i] starvation; these included the genes encoding a PitA-like [P.sub.i] uptake system and a putative [Na.sup.+]-dependent [P.sub.i] transporter and the genes involved in the metabolism of pyrophosphate and polyphosphate. In summary, a global, time-resolved picture of the response of C. glutamicum to [P.sub.i] starvation was obtained.
- Published
- 2003
134. Lateral flagellar gene system of Vibrio parahaemolyticus
- Author
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Stewart, Bonnie J. and McCarter, Linda L.
- Subjects
Gene mutations -- Physiological aspects ,Nucleotide sequence -- Genetic aspects ,Genetic regulation -- Analysis ,Sodium -- Physiological aspects ,Vibrio -- Genetic aspects ,Vibrio -- Growth ,Bacteriology -- Research ,Company growth ,Biological sciences - Abstract
Vibrio parahaemolyticus possesses dual flagellar systems adapted for movement under different circumstances. A single polar flagellum propels the bacterium in liquid (i.e., swimming) with a motor that is powered by the sodium motive force. Multiple proton-driven lateral flagella enable translocation over surfaces (i.e., swarming). The polar flagellum is produced continuously, while production of lateral flagella is induced when the organism is grown on surfaces. This work describes the isolation of mutants with insertions in the structural and regulatory laf genes. A Tn5-based lux transcriptional reporter transposon was constructed and used for mutagenesis and subsequent transcriptional analysis of the laf regulon. Twenty-nine independent insertions were distributed within 16 laf genes. DNA sequence analysis identified 38 laf genes in two loci. Among the mutants isolated, 11 contained surface-induced lux fusions. A hierarchy of laf gene expression was established following characterization of the laf::lux transcriptional fusion strains and by mutational and primer extension analyses of the laf regulon. The laf system is like many enteric systems in that it is a proton-driven, peritrichous flagellar system; however, laf regulation was different from the Salmonella-Escherichia coli paradigm. There is no apparent flhDC counterpart that encodes master regulators known to control flagellar biosynthesis and swarming in many enteric bacteria. A potential [[sigma].sup.54]-dependent regulator, LafK, was demonstrated to control expression of early genes, and a lateral-specific [[sigma].sup.28] factor controls late flagellar gene expression. Another notable feature was the discovery of a gene encoding a MotY-like product, which previously had been associated only with the architecture of sodium-type polar flagellar motors.
- Published
- 2003
135. CsrA regulates translation of the Escherichia coli carbon starvation gene, cstA, by blocking ribosome access to the cstA transcript
- Author
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Dubey, Ashok K., Baker, Carol S., Suzuki, Kazushi, Jones, Daniel A., Pandit, Pallavi, Romeo, Tony, and Babitzke, Paul
- Subjects
Carbon -- Physiological aspects ,Bacterial proteins -- Genetic aspects ,Peptides -- Genetic aspects ,Gene mutations -- Physiological aspects ,Escherichia coli -- Genetic aspects ,Genetic regulation -- Analysis ,Gene expression -- Physiological aspects ,Genetic transcription -- Physiological aspects ,Bacteriology -- Research ,Biological sciences - Abstract
CsrA is a global regulator that binds to two sites in the glgCAP leader transcript, thereby blocking ribosome access to the glgC Shine-Dalgarno sequence. The upstream CsrA binding site (GCACACGGAU) was used to search the Escherichia coli genomic sequence for other genes that might be regulated by CsrA. cstA contained an exact match that overlapped its Shine-Dalgarno sequence, cstA was previously shown to be induced by carbon starvation and to encode a peptide transporter. Expression of a cstA'-'lacZ translational fusion in wild-type and csrA mutant strains was examined. Expression levels in the csrA mutant were approximately twofold higher when cells were grown in Luria broth (LB) and 5- to 10-fold higher when LB was supplemented with glucose. It was previously shown that cstA is regulated by the cyclic AMP (cAMP)-cAMP receptor protein complex and transcribed by E[sigma.sup.70] We investigated the influence of [[sigma].sup.S] on cstA expression and found that a [[sigma].sup.S] deficiency resulted in a threefold increase in cstA expression in wild-type and csrA mutant strains; however, CsrA-dependent regulation was retained. The mechanism of CsrA-mediated cstA regulation was also examined in vitro. Cross-linking studies demonstrated that CsrA is a homodimer. Gel mobility shift results showed that CsrA binds specifically to cstA RNA, while coupled-transcription-translation and toeprint studies demonstrated that CsrA regulates CstA synthesis by inhibiting ribosome binding to cstA transcripts. RNA footprint and boundary analyses revealed three or four CsrA binding sites, one of which overlaps the cstA Shine-Dalgarno sequence, as predicted. These results establish that CsrA regulates translation of cstA by sterically interfering with ribosome binding.
- Published
- 2003
136. Chill induction of the SigB-dependent general stress response in Bacillus subtilis and its contribution to low-temperature adaptation
- Author
-
Brigulla, Matthias, Hoffmann, Tamara, Krisp, Andrea, Volker, Andrea, Bremer, Erhard, and Volker, Uwe
- Subjects
Genetic regulation -- Analysis ,Cold adaptation -- Physiological aspects ,Bacterial proteins -- Genetic aspects ,Western immunoblotting -- Usage ,Gene expression -- Physiological aspects ,Cells -- Genetic aspects ,Cells -- Growth ,Bacillus subtilis -- Genetic aspects ,Bacillus subtilis -- Physiological aspects ,Metabolism -- Genetic aspects ,Bacteriology -- Research ,Genetic transcription -- Physiological aspects ,Company growth ,Biological sciences - Abstract
A variety of environmental and metabolic cues trigger the transient activation of the alternative transcription factor SigB of Bacillus subtilis, which subsequently leads to the induction of more than 150 general stress genes. This general stress regulon provides nongrowing and nonsporulated cells with a multiple, nonspecific, and preemptive stress resistance. By a proteome approach we have detected the expression of the SigB regulon during continuous growth at low temperature (15[degrees]C). Using a combination of Western blot analysis and SigB-dependent reporter gene fusions, we provide evidence for high-level and persistent induction of the sigB operon and the SigB regulon, respectively, in cells continuously exposed to low temperatures. In contrast to all SigB-activating stimuli described thus far, induction by low temperatures does not depend on the positive regulatory protein RsbV or its regulatory phosphatases RsbU and RsbP, indicating the presence of an entirely new pathway for the activation of SigB by chill stress in B. subtilis. The physiological importance of the induction of the general stress response for the adaptation of B. subtilis to low temperatures is emphasized by the observation that growth of a sigB mutant is drastically impaired at 15[degrees]C. Inclusion of the compatible solute glycine betaine in the growth medium not only improved the growth of the wild-type strain but rescued the growth defect of the sigB mutant, indicating that the induction of the general stress regulon and the accumulation of glycine betaine are independent means by which B. subtilis cells cope with chill stress.
- Published
- 2003
137. The core dimerization domains of histidine kinases contain recognition specificity for the cognate response regulator
- Author
-
Ohta, Noriko and Newton, Austin
- Subjects
Bacterial proteins -- Genetic aspects ,Genetic regulation -- Analysis ,Genetic regulation -- Physiological aspects ,Cell cycle -- Genetic aspects ,Cell cycle -- Physiological aspects ,Cell division -- Genetic aspects ,Cell division -- Physiological aspects ,Histidine -- Physiological aspects ,Bacteriology -- Research ,Caulobacter ,Biological sciences - Abstract
Histidine kinases DivJ and PleC initiate signal transduction pathways that regulate an early cell division cycle step and the gain of motility later in the Caulobacter crescentus cell cycle, respectively. The essential single-domain response regulator DivK functions downstream of these kinases to catalyze phosphotransfer from DivJ and PleC. We have used a yeast two-hybrid screen to investigate the molecular basis of DivJ and PleC interaction with DivK and to identify other His-Asp signal transduction proteins that interact with DivK. The only His-Asp proteins identified in the two-hybrid screen were five members of the histidine kinase superfamily. The finding that most of the kinase clones isolated correspond to either DivJ or PleC supports the previous conclusion that DivJ and PleC are cognate DivK kinases. A 66-amino-acid sequence common to all cloned DivJ and PleC fragments contains the conserved helix 1, helix 2 sequence that forms a four-helix bundle in histidine kinases required for dimerization, autophosphorylation and phosphotransfer. We present results that indicate that the four-helix bundle subdomain is not only necessary for binding of the response regulator but also sufficient for in vivo recognition specificity between DivK and its cognate histidine kinases. The other three kinases identified in this study correspond to DivL, an essential tyrosine kinase belonging to the same kinase subfamily as DivJ and PleC, and the two previously uncharacterized, soluble histidine kinases CckN and CckO. We discuss the significance of these results as they relate to kinase response regulator recognition specificity and the fidelity of phosphotransfer in signal transduction pathways.
- Published
- 2003
138. YjdE (AdiC) is the arginine:agmatine antiporter essential for arginine-dependent acid resistance in Escherichia coli
- Author
-
Gong, Shimei, Richard, Hope, and Foster, John W.
- Subjects
Genetic regulation -- Analysis ,Plasmids -- Genetic aspects ,Plasmids -- Physiological aspects ,Arginine -- Physiological aspects ,Glutamate -- Physiological aspects ,Hydrogen-ion concentration -- Physiological aspects ,Escherichia coli -- Genetic aspects ,Escherichia coli -- Physiological aspects ,Acids -- Physiological aspects ,Bacteriology -- Research ,Biological sciences - Abstract
To survive in extremely acidic conditions, Escherichia coil has evolved three adaptive acid resistance strategies thought to maintain internal pH. While the mechanism behind acid resistance system 1 remains enigmatic, systems 2 and 3 are known to require external glutamate (system 2) and arginine (system 3) to function. These latter systems employ specific amino acid decarboxylases and putative antiporters that exchange the extracellular amino acid substrate for the intracellular by-product of decarboxylation. Although GadC is the predicted antiporter for system 2, the antiporter specific for arginine/agmatine exchange has not been identified. A computer-based homology search revealed that the yjdE (now called adiC) gene product shared an overall amino acid identity of 22% with GadC. A series of adiC mutants isolated by random mutagenesis and by targeted deletion were shown to be defective in arginine-dependent acid resistance. This defect was restored upon introduction of an adi[C.sup.+]-containing plasmid. An adiC mutant proved incapable of exchanging extracellular arginine for intracellular agmatine but maintained wild-type levels of arginine decarboxylase protein and activity. Western blot analysis indicated AdiC is an integral membrane protein. These data indicate that the arginine-to-agmatine conversion defect of adiC mutants was at the level of transport. The adi gene region was shown to be organized into two transcriptional units, adiAY and adiC, which are coordinately regulated but independently transcribed. The data also illustrate that the AdiA decarboxylase: AdiC antiporter system is designed to function only at acid levels sufficient to harm the ceil.
- Published
- 2003
139. Gene expression analysis of energy metabolism mutants of Desulfovibrio vulgaris hildenborough indicates an important role for alcohol dehydrogenase
- Author
-
Haveman, Shelley A., Brunelle, Veronique, Voordouw, Johanna K., Voordouw, Gerrit, Heidelberg, John F., and Rabus, Ralf
- Subjects
Metabolism -- Genetic aspects ,Sulfates -- Physiological aspects ,Cytology -- Physiological aspects ,Cytology -- Genetic aspects ,Genetic regulation -- Analysis ,Alcohol, Denatured -- Physiological aspects ,Alcohol -- Physiological aspects ,Bacterial proteins -- Genetic aspects ,Gene expression -- Physiological aspects ,DNA -- Genetic aspects ,Bacteria -- Genetic aspects ,Bacteria -- Growth ,Bacteriology -- Research ,Gene mutations -- Physiological aspects ,Company growth ,Biological sciences - Abstract
Comparison of the proteomes of the wild-type and Fe-only hydrogenase mutant strains of Desulfovibrio vulgaris Hildenborough, grown in lactate-sulfate (LS) medium, indicated the near absence of open reading frame 2977 (ORF2977)-coded alcohol dehydrogenase in the hyd mutant. Hybridization of labeled cDNA to a macroarray of 145 PCR-amplified D. vulgaris genes encoding proteins active in energy metabolism indicated that the adh gene was among the most highly expressed in wild-type cells grown in LS medium. Relative to the wild type, expression of the adh gene was strongly downregulated in the hyd mutant, in agreement with the proteomic data. Expression was upregulated in ethanol-grown wild-type cells. An adh mutant was constructed and found to be incapable of growth in media in which ethanol was both the carbon source and electron donor for sulfate reduction or was only the carbon source, with hydrogen serving as electron donor. The hyd mutant also grew poorly on ethanol, in agreement with its low level of adh gene expression. The adh mutant grew to a lower final cell density on LS medium than the wild type. These results, as well as the high level of expression of adh in wild-type cells on media in which lactate, pyruvate, formate, or hydrogen served as the sole electron donor for sulfate reduction, indicate that ORF2977 Adh contributes to the energy metabolism of D. vulgaris under a wide variety of metabolic conditions. A hydrogen cycling mechanism is proposed in which protons and electrons originating from cytoplasmic ethanol oxidation by ORF2977 Adh are converted to hydrogen or hydrogen equivalents, possibly by a putative [H.sub.2]-heterodisulfide oxidoreductase complex, which is then oxidized by periplasmic Fe-only hydrogenase to generate a proton gradient.
- Published
- 2003
140. PlmA, a new member of the GntR family, has plasmid maintenance functions in Anabaena sp. strain PCC 7120
- Author
-
Lee, Martin H., Scherer, Michael, Rigali, Sebastien, and Golden, James W.
- Subjects
Genetic regulation -- Analysis ,Phylogeny -- Genetic aspects ,Gene expression -- Physiological aspects ,Gene mutations -- Physiological aspects ,Plasmids -- Genetic aspects ,Chromosomes -- Genetic aspects ,Cyanobacteria -- Genetic aspects ,Bacteriology -- Research ,Biological sciences - Abstract
The filamentous cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 maintains a genome that is divided into a 6.4-Mb chromosome, three large plasmids of more that 100 kb, two medium-sized plasmids of 55 and 40 kb, and a 5.5-kb plasmid. Plasmid copy number can be dynamic in some cyanobacterial species, and the genes that regulate this process have not been characterized. Here we show that mutations in an open reading frame, all1076, reduce the numbers of copies per chromosome of several plasmids. In a mutant strain, plasmids pCC7120[delta] and pCC7120[zeta] are both reduced to less than 50% of their wild-type levels. The exogenous pDU1-based plasmid pAM1691 is reduced to less than 25% of its wild-type level, and the plasmid is rapidly lost. The peptide encoded by all1076 shows similarity to members of the GntR family of transcriptional regulators. Phylogenetic analysis reveals a new domain topology within the GntR family. PlmA homologs, all coming from cyanobacterial species, form a new subfamily that is distinct from the previously identified subfamilies. The all1076 locus, named plmA, regulates plasmid maintenance functions in Anabaena sp. strain PCC 7120.
- Published
- 2003
141. Expression of the secondary sigma factor [[sigma].sup.X] in Streptococcus pyogenes is restricted at two levels
- Author
-
Opdyke, Jason A., Scott, June R., and Moran, Charles P., Jr.
- Subjects
Gene expression -- Physiological aspects ,Genetic transcription -- Physiological aspects ,Gene mutations -- Physiological aspects ,Virulence (Microbiology) -- Research ,Genetic regulation -- Analysis ,Bacterial proteins -- Genetic aspects ,RNA polymerases -- Genetic aspects ,Bacteriology -- Research ,Streptococcus pyogenes -- Genetic aspects ,Biological sciences - Abstract
Secondary RNA polymerase sigma factors in many bacteria are responsible for regulating a vast range of processes including virulence. A protein ([[sigma].sup.x]) in the gram-positive human pathogen Streptococcus pyogenes (the group A Streptococcus or GAS) was recently shown to function in vitro as a secondary sigma factor. We report here the isolation of a mutant in which both sigX genes are inactivated, show that [[sigma].sup.x] functions in GAS cells, and show that the amount of [[sigma].sup.x] is controlled at two levels. Primer extension analysis indicates that sigX transcription is low in GAS cells grown in Todd-Hewitt yeast broth, and immunoblot assays with a [[sigma].sup.x]-specific polyclonal antibody demonstrate that the protein does not accumulate in these cells. To increase the level of sigX transcription in GAS, we constructed a strain that constitutively expresses the sigX gene from a heterologous promoter. Expression of sigX from this promoter led to transcription of the [[sigma].sup.x]-dependent cinA promoter in GAS cells. We found that expression of the sigX gene in a clpP mutant strain resulted in greater accumulation of [[sigma].sup.x] protein, which resulted in higher levels of transcription from the [[sigma].sup.x]-dependent promoters cinA, smf, and cglA. In addition, a clpP mutant containing sigX only at its wild-type loci on the chromosome generated more transcription from the [[sigma].sup.x]-dependent cinA promoter than did the wild-type parental strain. Therefore, [[sigma].sup.x] activity in GAS is limited by low-level transcription of the sigX structural genes and by clpP, which appears to negatively regulate [[sigma].sup.x] accumulation.
- Published
- 2003
142. NhaR and RcsB independently regulate the osmCpl promoter of Escherichia coli at overlapping regulatory sites
- Author
-
Sturny, Rachel, Cam, Kaymeuang, Gutierrez, Claude, and Conter, Annie
- Subjects
Enterobacteriaceae -- Genetic aspects ,Enterobacter -- Genetic aspects ,DNA -- Genetic aspects ,Genetic regulation -- Analysis ,Gene expression -- Physiological aspects ,Escherichia coli -- Genetic aspects ,Genetic transcription -- Physiological aspects ,Bacteriology -- Research ,Biological sciences - Abstract
Transcription of the Escherichia coli osmC gene is induced by several stress conditions, osmC is expressed from two overlapping promoters, osmCpl and osmCp2. The proximal promoter, osmCp2, is transcribed at the entry into the stationary phase by the [[sigma].sup.s] sigma factor. The distal promoter, osmCpl, is activated by NhaR and RcsB. NhaR is a positive regulator of the LysR family and is known to be an activator of the nhaA gene encoding an [Na.sup.+]/[H.sup.+] antiporter. RcsB is the response regulator of the RcsCDB His-Asp phosphorelay signal transduction system. Genetic data indicated that activation of osmCpl by both NhaR and RcsB requires the same short sequences upstream of the--35 region of the promoter. Accordingly, DNase I footprint analysis indicated that both activators protect an overlapping region close to the--35 box of the promoter and suggested that the regulatory effect is direct. Despite the overlap of the binding sites, each activator acts independent of the other and is specific for a particular stress. NhaR can stimulate osmCpl in response to an osmotic signal even in the absence of RcsB. RcsB is responsible for the induction of osmCpl by alteration of the cell envelope, even in the absence of NhaR. osmCpl as an example of multiple-stress-responsive promoter is discussed in light of a comparison of the NhaR and RcsB target regions in the Enterobacteriaceae.
- Published
- 2003
143. Membrane binding by MinD involves insertion of hydrophobic residues within the C-terminal amphipathic helix into the bilayer
- Author
-
Zhou, Huaijin and Lutkenhaus, Joe
- Subjects
Membranes (Biology) -- Genetic aspects ,Fluorescence spectroscopy -- Usage ,Gene mutations -- Physiological aspects ,Tryptophan -- Physiological aspects ,Adenosine triphosphatase -- Genetic aspects ,Adenosine triphosphatase -- Physiological aspects ,Phospholipids -- Genetic aspects ,Genetic regulation -- Analysis ,Bacteriology -- Research ,Biological sciences - Abstract
MinD binds to phospholipid vesicles in the presence of ATP and is released by MinE, which stimulates the MinD ATPase. Membrane binding requires a short conserved C-terminal region, which has the potential to form an amphipathic helix. This finding has led to a model in which the binding of ATP regulates the formation or accessibility of this helix, which then embeds in the membrane bilayer. To test this model, we replaced each of the four hydrophobic residues within this potential helix with tryptophan or a charged residue. Introduction of a negatively charged amino acid decreased membrane binding of Mind and its ability to activate MinC. In contrast, mutants with tryptophan substitutions retained the ability to bind to the membrane and activate MinC. Fluorescence emission spectroscopy analysis of the tryptophan mutants F263W, L264W, and L267W confirmed that these tryptophan residues did insert into the hydrophobic interior of the bilayer. We conclude that membrane binding by MinD involves penetration of the hydrophobic residues within the C-terminal amphipathic helix into the hydrophobic interior of the bilayer.
- Published
- 2003
144. Regulation of phospholipase D1 subcellular cycling through coordination of multiple membrane association motifs
- Author
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Du, Guangwei, Altshuller, Yelena M., Vitale, Nicolas, Huang, Ping, Chasserot-Golaz, Sylvette, Morris, Andrew J., Bader, Marie-France, and Frohman, Michael A.
- Subjects
Phospholipases -- Genetic aspects ,Phospholipases -- Physiological aspects ,Exocytosis -- Genetic aspects ,Genetic regulation -- Analysis ,Lipids -- Genetic aspects ,Lipids -- Physiological aspects ,Golgi apparatus -- Analysis ,Cell membranes -- Physiological aspects ,Cell membranes -- Genetic aspects ,Enzymes -- Physiological aspects ,Enzymes -- Genetic aspects ,Cytology -- Research ,Biological sciences - Abstract
The signaling enzyme phospholipase D1 (PLD1) facilitates membrane vesicle trafficking. Here, we explore how PLD1 subcellular localization is regulated via Phox homology (PX) and pleckstrin homology (PH) domains and a PI4,5[P.sub.2]-binding site critical for its activation. PLD1 localized to perinuclear endosomes and Golgi in COS-7 cells, but on cellular stimulation, translocated to the plasma membrane in an activity-facilitated manner and then returned to the endosomes. The PI4,5[P.sub.2]-interacting site sufficed to mediate outward translocation and association with the plasma membrane. However, in the absence of PX and PH domains, PLD1 was unable to return efficiently to the endosomes. The PX and PH domains appear to facilitate internalization at different steps. The PH domain drives PLD1 entry into lipid rafts, which we show to be a step critical for internalization. In contrast, the PX domain appears to mediate binding to PI5P, a lipid newly recognized to accumulate in endocytosing vesicles. Finally, we show that the PH domain--dependent translocation step, but not the PX domain, is required for PLD1 to function in regulated exocytosis in PC12 cells. We propose that PLD1 localization and function involves regulated and continual cycling through a succession of subcellular sites, mediated by successive combinations of membrane association interactions.
- Published
- 2003
145. Homer 2 tunes G protein-coupled receptors stimulus intensity by regulating RGS proteins and PLC[beta] GAP activities
- Author
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Min Shin, Dong, Dehoff, Marlin, Luo, Xiang, Kang, Shin Hyeok, Tu, Jiangchen, Nayak, Surendra K., Ross, Elliott M., Worley, Paul F., and Muallem, Shmuel
- Subjects
Chemical inhibitors -- Physiological aspects ,Phosphates -- Physiological aspects ,Genetic regulation -- Analysis ,G proteins -- Genetic aspects ,G proteins -- Physiological aspects ,Cytology -- Research ,Biological sciences - Abstract
Homers are scaffolding proteins that bind G protein-coupled receptors (GPCRs), inositol 1,4,5-triphosphate ([IP.sub.3]) receptors ([IP.sub.3]Rs), ryanodine receptors, and TRP channels. However, their role in [Ca.sub.2+] signaling in vivo is not known. Characterization of [Ca.sub.2+] signaling in pancreatic acinar cells from Homer[2.sup.-/-] and Homer[3.sup.-/-] mice showed that Homer 3 has no discernible role in [Ca.sup.2+] signaling in these cells. In contrast, we found that Homer 2 tunes intensity of [Ca.sup.2+] signaling by GPCRs to regulate the frequency of [Ca.sup.2+]; oscillations. Thus, deletion of Homer 2 increased stimulus intensity by increasing the potency for agonists acting on various GPCRs to activate PLC[beta] and evoke [Ca.sup.2+] release and oscillations. This was not due to aberrant localization of I[P.sub.3]Rs in cellular microdomains or I[P.sub.3]R channel activity. Rather, deletion of Homer 2 reduced the effectiveness of exogenous regulators of G proteins signaling proteins (RGS) to inhibit [Ca.sup.2+] signaling in vivo. Moreover, Homer 2 preferentially bound to PLC[beta] in pancreatic acini and brain extracts and stimulated GAP activity of RGS4 and of PLC[beta] in an in vitro reconstitution system, with minimal effect on PLC3-mediated PI[P.sub.2] hydrolysis. These findings describe a novel, unexpected function of Homer proteins, demonstrate that RGS proteins and PLC[beta] GAP activities are regulated functions, and provide a molecular mechanism for tuning signal intensity generated by GPCRs and, thus, the characteristics of [Ca.sup.2+]; oscillations.
- Published
- 2003
146. Activity of Rho-family GTPases during cell division as visualized with FRET-based probes
- Author
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Yoshizaki, Hisayoshi, Ohba, Yusuke, Kurokawa, Kazuo, Itoh, Reina E., Nakamura, Takeshi, Mochizuki, Naoki, Nagashima, Kazuo, and Matsuda, Michiyuki
- Subjects
Genetic regulation -- Analysis ,Cell membranes -- Genetic aspects ,Cell membranes -- Physiological aspects ,Proteins -- Physiological aspects ,Proteins -- Genetic aspects ,Nucleotides -- Genetic aspects ,Nucleotides -- Physiological aspects ,Guanine -- Physiological aspects ,Cell division -- Genetic aspects ,Cell division -- Physiological aspects ,Guanosine triphosphatase -- Genetic aspects ,Guanosine triphosphatase -- Physiological aspects ,Cytology -- Research ,Biological sciences - Abstract
Rho-family GTPases regulate many cellular functions. To visualize the activity of Rho-family GTPases in living cells, we developed fluorescence resonance energy transfer (FRET)-based probes for Rac1 and Cdc42 previously (Itoh, R.E., K. Kurokawa, Y. Ohba, H. Yoshizaki, N. Mochizuki, and M. Matsuda. 2002. Mol. Cell. Biol. 22:6582-6591). Here, we added two types of probes for RhoA. One is to monitor the activity balance between guanine nucleotide exchange factors and GTPase-activating proteins, and another is to monitor the level of GTP-RhoA. Using these FRET probes, we imaged the activities of Rho-family GTPases during the cell division of HeLa cells. The activities of RhoA, Rac1, and Cdc42 were high at the plasma membrane in interphase, and decreased rapidly on entry into M phase. From after anaphase, the RhoA activity increased at the plasma membrane including cleavage furrow. Rac1 activity was suppressed at the spindle midzone and increased at the plasma membrane of polar sides after telophase. Cdc42 activity was suppressed at the plasma membrane and was high at the intracellular membrane compartments during cytokinesis. In conclusion, we could use the FRET-based probes to visualize the complex spatio-temporal regulation of Rho-family GTPases during cell division.
- Published
- 2003
147. PhoP-responsive expression of the Salmonella enterica serovar Typhimurium slyA gene
- Author
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Norte, Valia A., Stapleton, Melanie R., and Green, Jeffrey
- Subjects
Bacteriophages -- Genetic aspects ,DNA -- Genetic aspects ,Genetic regulation -- Analysis ,Genetic transcription -- Physiological aspects ,Salmonella typhimurium -- Genetic aspects ,Gene expression -- Physiological aspects ,Microbial populations -- Genetic aspects ,Bacteriology -- Research ,Biological sciences - Abstract
The SlyA protein of Salmonella enterica serovar Typhimuriuim is a member of the MarR family of transcription regulators and is required for virulence and survival in professional macrophages. Isolated SlyA protein was able to bind a specific DNA target without posttranslational modification. This suggested that SlyA might not be activated by directly sensing an external signal but rather that the intracellular concentration of SlyA is enhanced in appropriate environments through the action of other transcription factors. Analysis of slyA transcription reveals the presence of a promoter region located upstream of the previously recognized SlyA repressed promoter. The newly identified upstream promoter region did not respond to SlyA but was activated by Mg(II) starvation in a PhoP-dependent manner. We present here evidence for a direct link between two transcription factors (PhoP and SlyA) crucial for Salmonella virulence.
- Published
- 2003
148. Cysteine-scanning analysis of the dimerization domain of EnvZ, an osmosensing histidine kinase
- Author
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Qin, Ling, Cai, Shengjian, Zhu, Yan, and Inouye, Masayori
- Subjects
Cysteine -- Genetic aspects ,Cysteine -- Physiological aspects ,Osmoregulation -- Analysis ,Water-electrolyte balance (Physiology) -- Analysis ,Gene mutations -- Physiological aspects ,Bacterial proteins -- Genetic aspects ,Escherichia coli -- Genetic aspects ,Genetic regulation -- Analysis ,Microbial populations -- Genetic aspects ,Bacteriology -- Research ,Biological sciences - Abstract
EnvZ and OmpR are a transmembrane sensor and its cognate response regulator, respectively, regulating the transcription of porin genes in response to medium osmolarity in Escherichia coli. The cytoplasmic domain of EnvZ (EnvZc) possesses both kinase and phosphatase activities and can be dissected into two functional domains, A and B. Here, we performed a cysteine-scanning analysis of domain A, a 67-residue central dimerization and phosphatase domain containing His-243 as the phosphorylation site, and we examined the effects of the cysteine substitution mutations on the enzymatic activities of domain A. The substitution mutations were made at 31 residues, from which 24 mutant domain A proteins were biochemically characterized. From the analysis of the phosphatase activity of purified mutant proteins, it was found that there are two regions in domain A which are important for this activity. Cysteine mutations in these regions dramatically reduce or completely abolish the phosphatase activity of domain A. The mutations that have the most-severe effects on domain A phosphatase activity also significantly reduce the phosphatase activity of EnvZc containing the same mutation. Using an in vitro complementation system with EnvZc(H243V), these cysteine mutants were further characterized for their autophosphorylation activities as well as their phosphotransfer activities. The results indicate that some mutations are specific either for the phosphatase activity or for the kinase activity.
- Published
- 2003
149. Role of feedback regulation of pantothenate kinase (CoaA) in control of coenzyme A levels in Escherichia coli
- Author
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Rock, Charles O., Park, Hee-Won, and Jackowski, Suzanne
- Subjects
Coenzymes -- Genetic aspects ,Genetic regulation -- Analysis ,Gene mutations -- Physiological aspects ,Biosynthesis -- Analysis ,Escherichia coli -- Genetic aspects ,Escherichia coli -- Physiological aspects ,Cytology -- Genetic aspects ,Microbial populations -- Genetic aspects ,Bacteriology -- Research ,Biological sciences - Abstract
Pantothenate kinase (CoaA) is a key regulator of coenzyme A (CoA) biosynthesis in Escherichia coli, and its activity is controlled by feedback inhibition by CoA and its thioesters. The importance of feedback inhibition in the control of the intracellular CoA levels was tested by constructing three site-directed mutants of CoaA that were predicted to be feedback resistant based on the crystal structure of the CoaA-CoA binary complex. CoaA[R106A], CoaA[H177Q], and CoaA[F247V] were purified and shown to retain significant catalytic activity and be refractory to inhibition by CoA. CoaA[R106A] retained 50% of the catalytic activity of CoaA, whereas the CoaA[H177Q] and CoaA[F247V] mutants were less active. The importance of feedback control of CoaA to the intracellular CoA levels was assessed by expressing either CoaA or CoaA[R106A] in strain ANS3 [coaA15(Ts) panD2]. Cells expressing CoaA[R106A] had significantly higher levels of phosphorylated pantothenate-derived metabolites and CoA in vivo and excreted significantly more 4'-phosphopantetheine into the medium compared to cells expressing the wild-type protein. These data illustrate the key role of feedback regulation of pantothenate kinase in the control of intracellular CoA levels.
- Published
- 2003
150. Transcription of bacteriophage PM2 involves phage-encoded regulators of heterologous origin
- Author
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Mannisto, Riina H., Grahn, A. Marika, Bamford, Dennis H., and Bamford, Jaana K.H.
- Subjects
Zinc finger proteins -- Genetic aspects ,Genetic regulation -- Analysis ,Membrane proteins -- Genetic aspects ,DNA -- Genetic aspects ,Bacteriophages -- Genetic aspects ,Genetic transcription -- Physiological aspects ,Microbial populations -- Genetic aspects ,Bacteriology -- Research ,Biological sciences - Abstract
Bacteriophage PM2 is the only described member of the Corticoviridae family. It is an icosahedral dsDNA virus with a membrane residing underneath the protein coat. PM2 infects some gram-negative Pseudoalteromonas spp. In the present study, we mapped the viral promoters and showed that the PM2 genome consists of three operons. Four new virus genes were assigned based on their function in transcription. Proteins P15 and P16 are shown to repress early transcription, and proteins P13 and P14 are shown to activate late transcription events. The early regulatory region, containing genes for proteins P15 and P16, as well as the newly identified early promoter region in PM2, has significant sequence similarity with the Pseudoalteromonas pAS28 plasmid. P14, the transcription activator for the structural genes, has a zinc finger motif homologous to archaeal and eukaryotic TFIIS-type regulatory factors.
- Published
- 2003
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