101. NIR-II Fluorescence Imaging Using Indocyanine Green Provides Early Prediction of Skin Avulsion-Injury in a Porcine Model
- Author
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Gao,Siqi, Yu,Yifeng, Wang,Zheng, Wu,Yifan, Qiu,Xingan, Jian,Chao, Yu,Aixi, Gao,Siqi, Yu,Yifeng, Wang,Zheng, Wu,Yifan, Qiu,Xingan, Jian,Chao, and Yu,Aixi
- Abstract
Siqi Gao,* Yifeng Yu,* Zheng Wang, Yifan Wu, Xingan Qiu, Chao Jian, Aixi Yu Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Aixi Yu; Chao Jian, Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, Peopleâs Republic of China, Tel +86-27-6781-3120 ; +86-27-6781-3120, Email yuaixi@whu.edu.cn; chaojian@whu.edu.cnPurpose: Currently, skin avulsionâinjury reconstruction is mainly based on subjective evaluation of traditional clinical signs. It frequently results in unnecessary tissue loss and incomplete debridement-related infection. This pilot study aimed to develop a novel near-infrared (NIR) II fluorescence imaging method to assess avulsed skinâperfusion status and thus predict its outcome early.Methods: Skin avulsionâinjury models were established by avulsing 10Ã 4 cm pedicled flaps on porcine hindlimbs. A clinically available improved NIR-&Igr;/II multispectral imaging system was applied for NIR imaging using indocyanine green (ICG) fluorescence. Continuous NIR-wavelength filters and dynamic imaging were used to investigate optimal imaging conditions and time window. NIR-&Igr;/II imaging was synchronously conducted for quality comparison of the two methods. Visual inspection and histological studies were used for assessing the final outcome of avulsed skin.Results: NIR-II fluorescence imaging with a 1,100 nm filter obtained satisfactory performance and reached maximum fluorescence intensity at 1 minute after ICG injection. NIR-II imaging clearly visualized the microvascular network in vascularized avulsed skin and revealed âdark areasâ in nonvascularized avulsed skin in a real-time fashion. NIR-II fluorescence imaging demonstrated higher resolution than NIR-I imaging, as indicated by ae higher signal-to-background
- Published
- 2022