Search

Your search keyword '"Gao, Daming"' showing total 452 results
Start Over Author "Gao, Daming"
452 results on '"Gao, Daming"'

102. Cullin5 deficiency promotes small-cell lung cancer metastasis by stabilizing integrin β1

Author

Zhao, Gaoxiang, primary, Gong, Liyan, additional, Su, Dan, additional, Jin, Yujuan, additional, Guo, Chenchen, additional, Yue, Meiting, additional, Yao, Shun, additional, Qin, Zhen, additional, Ye, Yi, additional, Tang, Ying, additional, Wu, Qibiao, additional, Zhang, Jian, additional, Cui, Binghai, additional, Ding, Qiurong, additional, Huang, Hsinyi, additional, Hu, Liang, additional, Chen, Yuting, additional, Zhang, Peiyuan, additional, Hu, Guohong, additional, Chen, Luonan, additional, Wong, Kwok-Kin, additional, Gao, Daming, additional, and Ji, Hongbin, additional

Published
2019
Full Text
View/download PDF

104. Akt finds its new path to regulate cell cycle through modulating Skp2 activity and its destruction by APC/Cdh1

Author

Tseng Alan, Inuzuka Hiroyuki, Gao Daming, and Wei Wenyi

Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671
Abstract

Abstract Skp2 over-expression has been observed in many human cancers. However, the mechanisms underlying elevated Skp2 expression have remained elusive. We recently reported that Akt1, but not Akt2, directly controls Skp2 stability by interfering with its association with APC/Cdh1. As a result, Skp2 degradation is protected in cancer cells with elevated Akt activity. This finding expands our knowledge of how specific kinase cascades influence proteolysis governed by APC/Cdh1 complexes. However, it awaits further investigation to elucidate whether the PI3K/Akt circuit affects other APC/Cdh1 substrates. Our results further strengthen the argument that different Akt isoforms might have distinct, even opposing functions in the regulation of cell growth or migration. In addition, we noticed that Ser72 is localized in a putative Nuclear Localization Sequence (NLS), and that phosphorylation of Ser72 disrupts the NLS and thus promotes Skp2 cytoplasmic translocation. This finding links elevated Akt activity with the observed cytoplasmic Skp2 staining in aggressive breast and prostate cancer patients. Furthermore, it provides the rationale for the development of specific Akt1 inhibitors as efficient anti-cancer therapeutic agents.

Published
2009
Full Text
View/download PDF

109. Ubiquitin-like proteins and their Chinese nomenclatures

Author

Wang, Chen, primary, Xie, Songbo, additional, Xu, Ping, additional, Rao, Feng, additional, Li, Wei, additional, Zhang, Hongbing, additional, Zhou, Jun, additional, Miao, Shiying, additional, Zhang, Lingqiang, additional, Wang, Hengbing, additional, Liu, Xuan, additional, Zhang, Hong, additional, Xie, Zhiping, additional, Xu, Zhi-Heng, additional, Cao, Cheng, additional, Wei, Wenyi, additional, Chen, Ceshi, additional, Wang, Zhanxin, additional, Chen, Quan, additional, Jin, Jianping, additional, Gao, Daming, additional, Feng, Rentian, additional, Yang, Chengwei, additional, Wang, Ping, additional, Liu, Cuihua, additional, Ying, Meidan, additional, Ma, Leina, additional, Jiang, Tian-Xia, additional, Sun, Yi, additional, Wang, Linfang, additional, Yang, Bo, additional, Song, Zhiyin, additional, Qiu, Xiao-Bo, additional, Li, Hui-Hua, additional, Shang, Yu, additional, Zhao, Yongchao, additional, Zhu, Jun, additional, Wan, Yong, additional, Chang, Zhijie, additional, Wu, Mian, additional, Hu, Ronggui, additional, Wu, Qiao, additional, Cheng, Jinke, additional, Jia, Lijun, additional, and Xie, Qi, additional

Published
2018
Full Text
View/download PDF

112. Optimization and Update of EAST In-Vessel Components in 2011

Author

Wang Shengming (王声铭), Fu Peng (傅鹏), Liu Xufeng, XU Tiejun (许铁军), Wan Bao-Nian, Song Yuntao, Wu Jiefeng, Ji Xiang, Zhang Ping, Zhu Ning (祝宁), Peng Xuebing, Liu Zhihong (刘志宏), Gao Daming, Cao Lei, Li Jiangang, Dai Yu (戴羽), Xu Weiwei, Shen Guang, Zhou Zibo, and Gong Xianzu

Subjects
Materials science, Long pulse, Hydrogen compounds, Power over, Safe operation, Field (physics), Nuclear engineering, Water cooling, Plasma, Condensed Matter Physics, Plasma current
Abstract

For safe operation with active water cooling plasma facing components (PFCs) to handle a large input power over a long pulse discharge, some design optimization, R&D and maintenance were accomplished to improve the in-vessel components. For the purpose of large plasma current (1 MA) operation, the previous separated top and bottom passive stabilizers in the low field were electrical connected to stabilize plasma in the case of vertical displace events (VDEs). The design and experiments are described in this paper

Published
2013

114. Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders

Author

Xu, Xingxing, primary, Li, Chuanyin, additional, Gao, Xiaobo, additional, Xia, Kun, additional, Guo, Hui, additional, Li, Yali, additional, Hao, Zijian, additional, Zhang, Lei, additional, Gao, Daming, additional, Xu, Chenfan, additional, Xu, Huatai, additional, Xiong, Zhi-Qi, additional, Qiu, Zilong, additional, Mei, Ling, additional, Xie, Xiaoduo, additional, Ruan, Kangcheng, additional, and Hu, Ronggui, additional

Published
2017
Full Text
View/download PDF

115. Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2

Author

Zhang, Shihao, primary, Chen, Qinghua, additional, Liu, Qingxu, additional, Li, Yuxi, additional, Sun, Xiufeng, additional, Hong, Lixin, additional, Ji, Suyuan, additional, Liu, Chengyan, additional, Geng, Jing, additional, Zhang, Weiji, additional, Lu, Zhonglei, additional, Yin, Zhen-Yu, additional, Zeng, Yuanyuan, additional, Lin, Kwang-Huei, additional, Wu, Qiao, additional, Li, Qiyuan, additional, Nakayama, Keiko, additional, Nakayama, Keiich I., additional, Deng, Xianming, additional, Johnson, Randy L., additional, Zhu, Liang, additional, Gao, Daming, additional, Chen, Lanfen, additional, and Zhou, Dawang, additional

Published
2017
Full Text
View/download PDF

116. Ubiquitylation of p62/sequestosome1 activates its autophagy receptor function and controls selective autophagy upon ubiquitin stress

Author

Peng, Hong, primary, Yang, Jiao, additional, Li, Guangyi, additional, You, Qing, additional, Han, Wen, additional, Li, Tianrang, additional, Gao, Daming, additional, Xie, Xiaoduo, additional, Lee, Byung-Hoon, additional, Du, Juan, additional, Hou, Jian, additional, Zhang, Tao, additional, Rao, Hai, additional, Huang, Ying, additional, Li, Qinrun, additional, Zeng, Rong, additional, Hui, Lijian, additional, Wang, Hongyan, additional, Xia, Qin, additional, Zhang, Xuemin, additional, He, Yongning, additional, Komatsu, Masaaki, additional, Dikic, Ivan, additional, Finley, Daniel, additional, and Hu, Ronggui, additional

Published
2017
Full Text
View/download PDF

119. Acetylation of PGK1 promotes liver cancer cell proliferation and tumorigenesis

Author

Hu, Hongli, primary, Zhu, Wenwei, additional, Qin, Jun, additional, Chen, Min, additional, Gong, Liyan, additional, Li, Long, additional, Liu, Xiangyuan, additional, Tao, Yongzhen, additional, Yin, Huiyong, additional, Zhou, Hu, additional, Zhou, Lisha, additional, Ye, Dan, additional, Ye, Qinghai, additional, and Gao, Daming, additional

Published
2016
Full Text
View/download PDF

120. Low Temperature Chemoselective Hydrogenation of Aldehydes over a Magnetic Pd Catalyst.

Author

Liu, Anqiu, Wang, Xiaochen, Gao, Daming, Wang, Le, Cheng, Junjie, Wang, An, and Zhang, Zehui

Subjects
LOW temperatures, HYDROGENATION, ALDEHYDES, HAMMETT equation, AROMATIC aldehydes, TOLUENE
Abstract

Chemoselective hydrogenation of aldehydes with heterogeneous catalysts under mild conditions is of great importance but remains a major challenge. Herein, an efficient strategy was developed for low temperature chemoselective hydrogenation of aldehydes with broad substrate scope over a magnetic material supported palladium catalyst (γ-Fe2O3@HAP-Pd). Aldehydes bearing various reducible functional groups readily underwent hydrogenation to give the corresponding primary alcohols with moderate to excellent yield at room temperature in aqueous solutions. The Hammett equation revealed that the hydrogenation of aromatic aldehydes proceeded via an anionic intermediate. Additionally, when the temperature increased to 70 °C, toluene was obtained by the deoxygenation of benzaldehyde in excellent yield. Furthermore, the γ-Fe2O3@HAP-Pd could be recycled up to six times without loss of activity and metal leaching. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

121. The batch production for superconducting magnet coils of EAST (HT-7U)

Author

Pan Wan-jiang, Li Bao-zeng, Gao Daming, Yu Jie, Wu Weiyue, Tao Yuming, Wu Jiefeng, Pan Yin-nian, Chen Siyue, Wen Jun, Wu Yu, Zhang Ping, and Zhu Wenhua

Subjects
Tokamak, Materials science, Mechanical Engineering, Nuclear engineering, Toroidal field, Superconducting magnet, Fusion power, Chinese academy of sciences, law.invention, Superconducting tokamak, Nuclear magnetic resonance, Nuclear Energy and Engineering, law, General Materials Science, Batch production, Control methods, Civil and Structural Engineering
Abstract

The experimental advanced superconducting tokamak (EAST) is being constructed at the Institute of Plasma Physics of Chinese Academy of Sciences (CASIPP). The EAST project, approved by the Chinese government as a national mega project of science research is a fully superconducting tokamak. The most key component for EAST is the superconducting magnet coils (SMCs), which consists of 16 toroidal field coils (TFCs) and 14 poloidal field coils (PFCs). In 2003, three prototypes, one TFC and two PFCs, were successfully completed and passed a series of cryogenic tests. Batch production, needed for the SMCs has begun at CASIPP since 2002. Up to now, all 58 CIC conductors with a total length of 32 km, 12 TFCs out of 16 and 10 PFCs out of 14 have been fabricated. This paper emphasizes on the various technology issues that must be faced and solved for four R&D lines of SMCs after transforming to batch production. Quality control methods in process are also described.

Published
2005

122. The Alignment and Assembly for EAST Tokamak Device

Author

Gao Daming, Chen Wen-ge, Wei Jing, Wu Weiyue, Weng Peide, and Wu Songtao

Subjects
Physics, Cryostat, Tokamak, business.industry, Process (computing), Superconducting magnet, Plasma, Condensed Matter Physics, law.invention, Optics, law, Thermal radiation, Shield, Millimeter, business
Abstract

EAST (HT-7U) is a large fusion experimental device. It is a full superconducting tokamak with 1 MA of plasma current, 1000 s of plasma duration, high elongation and triangularity. It mainly consists of superconducting magnets of poloidal and toroidal field (PF & TF), vacuum vessel (VV), thermal radiation shield (TRS) and cryostat vessel (CV). The significant difficulty for assembly of EAST is tight installation tolerances, which are in the order of several tenth of a millimeter. In particular, the alignment of plasma facing components to the magnetic axis of the device is less than ± 0.5 mm. At present, a reasonable assembly process of EAST has been defined, and based on it, the alignment method for EAST, including the survey control network, the location of the main components in different directions, the magnetic axis determination and the accurate positioning of the plasma facing components inside of the vacuum vessel and so on, has been defined by using the sophisticated optical metrology system (SOMS). This paper describes the assembly procedure of EAST and the installation tolerances associated with the main components. Meanwhile, how to establish the assembly survey control network, magnetic axis determination methods, are introduced in detail.

Published
2005

123. The Investigation on Welding Processes for SUS316LN Tubes Used in Superconducting Magnetic System of EAST

Author

Wu Jiefeng, Gao Daming, Weng Peide, and Chen Siyue

Subjects
Superconductivity, Materials science, Flow (psychology), Welding, engineering.material, Condensed Matter Physics, Square (algebra), law.invention, Conductor, law, Thin wall, engineering, Poloidal field, Austenitic stainless steel, Composite material
Abstract

The force flow cooled superconducting cable-in-conduit conductor (CICC) is used in both of EAST toroidal field (TF) and poloidal field (PF) coils. The conductor consists of multi-stage NbTi superconducting cable and 1.5 mm thick square jacket. The cable is pulled through in a thin wall circular jacket and then compacted to square cross-section conductor. The jacket material is SUS316LN austenitic stainless steel seamless tubes (about 10 m each), which is assembled by butt-welding up to 600 m. The results of the welding procedure investigation and quality assurance procedures carrying out are described in this paper.

Published
2005

124. A 600-m Jacketing Line of Cable-in-conduit Conductor (CICC) and the First 600-m Dummy Cable

Author

Gao Daming, Wang Hai-jing, He Wei, Wu Jiefeng, Zhang Pin, Chen Siyue, Chen Xing-qian, Yi Zian, and Xu Xue-fu

Subjects
Production line, Dye penetration, Electrical conduit, Materials science, Pressure control, Ultrasonic testing, Mechanical engineering, Condensed Matter Physics, Chinese academy of sciences, Leakage (electronics), Conductor
Abstract

A cable-in-conduit conductor (CICC) production line was designed and constructed in Institute of Plasma Physics of Chinese Academy of Sciences (IPPCAS) by the end of 2000. It can produce a length of 600 meters and three kinds of sections of 20.8±0.1 × 20.8±0.1, 20.4±0.1 × 20.4±0.1 and 18.6 ± 0.1 × 18.6 ± 0.1 mm2. If the rollers of the shaping machine are changed, it can also produce other sizes of CICCs. So-called inserting-cable technology is adopted in this production line, where the procedures consist of tube pre-treatment (cleaning, pressure and leakage testing, end cutting), conduits butt-welding, six kinds of quality checking (endoscopy, dye penetration, pressure control, leakage testing, ultrasonic inspection and x-ray testing), cable inserting, shaping (compacting & squaring), pre-bending & winding and final checking. Now all the instruments and facilities required for these technologies have been installed and got ready. Some key technologies have been explored and good results obtained. Some short samples were produced and a 600 meters long sample was made out in August, 2001.

Published
2002

125. Trial Manufacture of a 30 m Cable-in-Conduit-Conductor (CICC) for HT-7U

Author

Li Bao-zeng, Bi Yanfang, Xu Xue-fu, Wang Yong-cheng, Chen Xing-qian, Gao Daming, Weng Peide, Zhang Pin, and Wu Jiefeng

Subjects
Fabrication, Electrical conduit, law, Computer science, Mechanical engineering, Welding, Condensed Matter Physics, Engineering design process, law.invention, Conductor
Abstract

This paper reports the working procedure of a 30 m cable-in-conduit-conductor (CICC), which has been first successfully fabricated by a working group of IPP in Jan. 1999. A series of problems along with measures for their solution were put forward at the request of engineering design on the basis of an in-depth understanding of the basic performance of the related techniques for the manufacture of CICC. Through a full-scale trial manufacture of a 30 m conductor including a series of test on performance technology such as tube-tube butted welding, pulling, equaring, prebending and take-up, the trial manufacture of the first home-made CICC conductor has been accomplished, demonstrating that the fabrication of a long-length CICC is entirely possible theorically and practically through our own efforts. Results and problems concerned in this trial fabrication are also presented in the paper.

Published
2000

126. Development of Special Winding Machine for HT-7U Superconducting Tokamak

Author

Gao Daming, Yu Jie, He Wei, Wen Jun, Wang Hai-jing, Pan Yin-nian, Tao Yuming, Zhu Wenhua, and Cheng Leping

Subjects
Winding machine, Tokamak, Materials science, Electromagnetic coil, law, Magnet, Electrical equipment, Flatness (systems theory), Mechanical engineering, Servomotor, Condensed Matter Physics, Conductor, law.invention
Abstract

A special winding machine with high accuracy has just been developed and applied to the construction of HT-7U Tokamak. It is one of the critical facilities for R & D of HT-7U construction. The machine mainly consists of five parts, including a CICC pay-off spool, a four-roller correcting assembly, a four-roller forming/bending assembly, a continuous winding structure and a CNC control system with three-axis AC servo motors. The facility is used for Cable in Conduit Conductor (CICC) magnet fabrication of HT-7U. The main requirements of the winding machine are: continuous winding to reduce joints inside the coils; pre-forming CICC conductor to avoid winding with tension; suitable for all TF & PF coils of various coil shapes and within the dimension limit; improving the configuration tolerance and the special flatness of the CICC conductor. This paper emphasizes on the design and fabrication of the special winding machine for HT-7U. Some analyses and techniques in winding process for trial D-shape coil are also presented.

Published
2000

130. Computation of Isobaric Vapor-Liquid Equilibrium Data for Binary and Ternary Mixtures of Methanol, Water, and Ethanoic Acid from T, p, x, and HmE Measurements

Author

Gao, Daming, Zhang, Hui, Lücking, Peter, Sun, Hong, Si, Jingyu, Zhu, Dechun, Chen, Hong, and Shi, Jianjun

Subjects
Article Subject
Abstract

Vapor-liquid equilibrium (VLE) data for the strongly associated ternary system methanol + water + ethanoic acid and the three constituent binary systems have been determined by the total pressure-temperature-liquid-phase composition-molar excess enthalpy of mixing of the liquid phase (p, T, x, HmE) for the binary systems using a novel pump ebulliometer at 101.325 kPa. The vapor-phase compositions of these binary systems had been calculated from Tpx and HmE based on the Q function of molar excess Gibbs energy through an indirect method. Moreover, the experimental T, x data are used to estimate nonrandom two-liquid (NRTL), Wilson, Margules, and van Laar model parameters, and these parameters in turn are used to calculate vapor-phase compositions. The activity coefficients of the solution were correlated with NRTL, Wilson, Margules, and van Laar models through fitting by least-squares method. The VLE data of the ternary system were well predicted from these binary interaction parameters of NRTL, Wilson, Margules, and van Laar model parameters without any additional adjustment to build the thermodynamic model of VLE for the ternary system and obtain the vapor-phase compositions and the calculated bubble points.

Published
2012
Full Text
View/download PDF

132. ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer

Author

Ji, Shunrong, primary, Qin, Yi, additional, Shi, Si, additional, Liu, Xiangyuan, additional, Hu, Hongli, additional, Zhou, Hu, additional, Gao, Jing, additional, Zhang, Bo, additional, Xu, Wenyan, additional, Liu, Jiang, additional, Liang, Dingkong, additional, Liu, Liang, additional, Liu, Chen, additional, Long, Jiang, additional, Zhou, Haijun, additional, Chiao, Paul J, additional, Xu, Jin, additional, Ni, Quanxing, additional, Gao, Daming, additional, and Yu, Xianjun, additional

Published
2015
Full Text
View/download PDF

134. Phosphorylation by Akt1 Promotes Skp2 Cytoplasmic Localization and Impairs APC/Cdh1-mediated Skp2 Destruction

Author

Gao, Daming, Inuzuka, Hiroyuki, Tseng, Alan, Chin, Rebecca Y., Toker, Alex, and Wei, Wenyi

Subjects
Article
Abstract

Deregulated Skp2 function promotes cell transformation, and this is consistent with observations of Skp2 over-expression in many human cancers. However, the mechanisms underlying elevated Skp2 expression remain elusive. Here we report that the serine/threonine protein kinase Akt1, but not Akt2, directly controls Skp2 stability by a mechanism that involves degradation by the APC/Cdh1 ubiquitin ligase complex. We further show that Akt1 phosphorylates Skp2 at Ser72, which is required to disrupt the interaction between Cdh1 and Skp2. In addition, we show that Ser72 is localized within a putative Nuclear Localization Sequence (NLS) and that phosphorylation of Ser72 by Akt leads to Skp2 cytoplasmic translocation. This finding expands our knowledge of how specific signaling kinase cascades influence proteolysis governed by APC/Cdh1 complexes, and provides evidence that elevated Akt activity and cytoplasmic Skp2 expression may be causative for cancer progression.

Published
2009

138. Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders

Author

Xu, Xingxing, Li, Chuanyin, Gao, Xiaobo, Xia, Kun, Guo, Hui, Li, Yali, Hao, Zijian, Zhang, Lei, Gao, Daming, Xu, Chenfan, Xu, Huatai, Xiong, Zhi-Qi, Qiu, Zilong, Mei, Ling, Xie, Xiaoduo, Ruan, Kangcheng, and Hu, Ronggui

Abstract

The autism spectrum disorders (ASDs) are a collection of human neurological disorders with heterogeneous etiologies. Hyperactivity of E3 ubiquitin (Ub) ligase UBE3A, stemming from 15q11-q13 copy number variations, accounts for 1%-3% of ASD cases worldwide, but the underlying mechanisms remain incompletely characterized. Here we report that the functionality of ALDH1A2, the rate-limiting enzyme of retinoic acid (RA) synthesis, is negatively regulated by UBE3A in a ubiquitylation-dependent manner. Excessive UBE3A dosage was found to impair RA-mediated neuronal homeostatic synaptic plasticity. ASD-like symptoms were recapitulated in mice by overexpressing UBE3A in the prefrontal cortex or by administration of an ALDH1A antagonist, whereas RA supplements significantly alleviated excessive UBE3A dosage-induced ASD-like phenotypes. By identifying reduced RA signaling as an underlying mechanism in ASD phenotypes linked to UBE3A hyperactivities, our findings introduce a new vista of ASD etiology and facilitate a mode of therapeutic development against this increasingly prevalent disease.

Published
2018
Full Text
View/download PDF

139. Implementation of database in Web-based agriculture ES

Author

Gao Daming and Yang Panhong

Subjects
Database, Web development, Computer science, business.industry, computer.software_genre, Database testing, World Wide Web, Web page, Web application, The Internet, Web service, business, computer, Data Web
Abstract

It is a frequent and important operation to access a Web-based database and manage its data. The efficiency of this operation has an effect on the whole system in the performance to expense ratio. This paper presents an implementation of a database in a Web-based agriculture ES, with emphasis on discussing the realization that takes less time in accessing the Web-based database and converting the data, and elevates the performance to expense ratio of the whole system.

Published
2002

140. Investigating the Role of Akt1 in Prostate Cancer Development through Phosphorylation-dependent Regulation of Skp2 Stability and Oncogenic Function

Author

BETH ISRAEL DEACONESS MEDICAL CENTER BOSTON MA, Wei, Wenyi, Gao, Daming, Inuzuka, Hiroyuki, Tseng, Alan, BETH ISRAEL DEACONESS MEDICAL CENTER BOSTON MA, Wei, Wenyi, Gao, Daming, Inuzuka, Hiroyuki, and Tseng, Alan

Abstract

Elevated Skp2 expression is frequently observed in many tumors including breast and prostate carcinomas. However, the molecular mechanisms underlying elevated Skp2 expression in prostate cancers have not been fully explored. Hyperactivation of the Akt pathway is considered a hallmark of many cancers and it has been reported that activation of the PI3K/Akt pathway enhances p27 destruction. Thus, we hypothesize that sustained Akt activity in prostate cancer cells leads to elevated phosphorylation of Skp2, and subsequently influences Skp2 stability and its oncogenic functions. In support of our hypothesis, we found that Skp2 abundance is affected by manipulation of the PTEN/PI3K/Akt pathway in the PC3 and LNCaP prostate cancer cell lines. Furthermore, we showed that this is partially through phosphorylation of Skp2 by Akt, which impairs Skp2 destruction by Cdh1. Sequence analysis revealed that the Akt phosphorylation site (Ser72) is localized in a putative Nuclear Localization Sequence (NLS). Consistent with this notion, we found that overexpression of Myr-Akt promoted Skp2 cytoplasmic translocation, and that inhibition of PI3K/Akt activity enhanced its nuclear localization. We believe that these studies will provide a novel mechanism for Skp2 overexpression in prostate cancers and provide the rationale for developing Akt1-specific inhibitors to treat prostate cancer patients., The original document contains color images.

Published
2012

141. Investigating the Role of Akt1 in Prostate Cancer Development Through Phosphorylation-Dependent Regulation of Skp2 Stability and Oncogenic Function

Author

BETH ISRAEL DEACONESS MEDICAL CENTER BOSTON MA, Gao, Daming, Inuzuka, Hiroyuki, Tseng, Alan, Wei, Wenyi, BETH ISRAEL DEACONESS MEDICAL CENTER BOSTON MA, Gao, Daming, Inuzuka, Hiroyuki, Tseng, Alan, and Wei, Wenyi

Abstract

Elevated Skp2 expression is frequently observed in many tumors including breast and prostate carcinomas. However, the molecular mechanisms underlying elevated Skp2 expression in prostate cancers have not been fully explored. Hyperactivation of the Akt pathway is considered a hallmark of many cancers and it has been reported that activation of the PI3K/Akt pathway enhances p27 destruction. Thus, we hypothesize that sustained Akt activity in prostate cancer cells leads to elevated phosphorylation of Skp2, and subsequently influences Skp2 stability and its oncogenic functions. In support of our hypothesis, we found that Skp2 abundance is affected by manipulation of the PTEN/PI3K/Akt pathway in the PC3 and LNCaP prostate cancer cell lines. Furthermore, we showed that this is partially through phosphorylation of Skp2 by Akt, which impairs Skp2 destruction by Cdh1. Sequence analysis revealed that the Akt phosphorylation site (Ser72) is localized in a putative Nuclear Localization Sequence (NLS). Consistent with this notion, we found that overexpression of Myr-Akt promoted Skp2 cytoplasmic translocation, and that inhibition of PI3K/Akt activity enhanced its nuclear localization. We believe that these studies will provide a novel mechanism for Skp2 overexpression in prostate cancers and provide the rationale for developing Akt1-specific inhibitors to treat prostate cancer patients., The original document contains color images.

Published
2010

142. Sin1 phosphorylation impairs mTORC2 complex integrity and inhibits downstream Akt signalling to suppress tumorigenesis

Author

Liu, Pengda, primary, Gan, Wenjian, additional, Inuzuka, Hiroyuki, additional, Lazorchak, Adam S., additional, Gao, Daming, additional, Arojo, Omotooke, additional, Liu, Dou, additional, Wan, Lixin, additional, Zhai, Bo, additional, Yu, Yonghao, additional, Yuan, Min, additional, Kim, Byeong Mo, additional, Shaik, Shavali, additional, Menon, Suchithra, additional, Gygi, Steven P., additional, Lee, Tae Ho, additional, Asara, John M., additional, Manning, Brendan D., additional, Blenis, John, additional, Su, Bing, additional, and Wei, Wenyi, additional

Published
2013
Full Text
View/download PDF

147. Optimization and Update of EAST In-Vessel Components in 2011

Author

Ji, Xiang, primary, Song, Yuntao, additional, Shen, Guang, additional, Cao, Lei, additional, Zhou, Zibo, additional, Xu, Tiejun, additional, Liu, Xufeng, additional, Xu, Weiwei, additional, Peng, Xuebing, additional, Wang, Shengming, additional, Zhang, Ping, additional, Zhu, Ning, additional, Dai, Yu, additional, Liu, Zhihong, additional, Wu, Jiefeng, additional, Gao, Daming, additional, Gong, Xianzu, additional, Fu, Peng, additional, Wan, Baonian, additional, and Li, Jiangang, additional

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results