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1,222 results on '"Friml, Jiří"'

102. The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport for root growth adaptation.

Author

Yalu Wang, Zhi Yuan, Jinyi Wang, Huixin Xiao, Lu Wan, Lanxin Li, Yan Guo, Zhizhong Gong, Friml, Jiří, and Jing Zhang

Subjects
ROOT growth, AUXIN, ARABIDOPSIS thaliana, PLANT nutrients, NITRATES
Abstract

As a crucial nitrogen source, nitrate (NO3-) is a key nutrient for plants. Accordingly, root systems adapt to maximize NO3- availability, a developmental regulation also involving the phytohormone auxin. Nonetheless, the molecular mechanisms underlying this regulation remain poorly understood. Here, we identify low-nitrate-resistant mutant (lonr) in Arabidopsis (Arabidopsis thaliana), whose root growth fails to adapt to low-NO3- conditions. lonr2 is defective in the high-affinity NO3- transporter NRT2.1. lonr2 (nrt2.1) mutants exhibit defects in polar auxin transport, and their low-NO3--induced root phenotype depends on the PIN7 auxin exporter activity. NRT2.1 directly associates with PIN7 and antagonizes PIN7-mediated auxin efflux depending on NO3- levels. These results reveal a mechanism by which NRT2.1 in response to NO3- limitation directly regulates auxin transport activity and, thus, root growth. This adaptive mechanism contributes to the root developmental plasticity to help plants cope with changes in NO3- availability. [ABSTRACT FROM AUTHOR]

Published
2023
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121. Auxin analog-induced Ca(2+) signaling is not involved in inhibition of endosomal aggregation

Author

Wang, Ren, Himschoot, Ellie, Grenzi, Matteo, Chen, Jian, Safi, Alaeddine, Krebs, Melanie, Schumacher, Karin, Nowack, Moritz K., Van Damme, Daniёl, De Smet, Ive, Geelen, Danny, Beeckman, Tom, Friml, Jiří, Costa, Alex, and Vanneste, Steffen

Subjects
Indoleacetic Acids, Plant Growth Regulators, Arabidopsis Proteins, Arabidopsis, Calcium, Plant Roots, Article
Abstract

Much of what we know about the role of auxin in plant development derives from exogenous manipulations of auxin distribution and signaling, using inhibitors, auxins and auxin analogs. In this context, synthetic auxin analogs, such as 1-Naphtalene Acetic Acid (1-NAA), are often favored over the endogenous auxin indole-3-acetic acid (IAA), in part due to their higher stability. While such auxin analogs have proven to be instrumental to reveal the various faces of auxin, they displays in some cases distinct bioactivities compared to IAA. Here, we focused on the effect of auxin analogs on the accumulation of PIN proteins in Brefeldin A (BFA)-sensitive endosomal aggregations (BFA bodies), and the correlation with the ability to elicit Ca(2+) responses. For a set of commonly used auxin analogs, we evaluated if auxin-analog induced Ca(2+) signaling inhibits PIN accumulation in. We found that not all auxin analogs elicited a Ca(2+) response, and that their differential ability to elicit Ca(2+) responses correlated partially with their ability to inhibit BFA-body formation. However, in tir1/afb and cngc14, 1-NAA-induced Ca(2+) signaling was strongly impaired, while 1-NAA’s still could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent Ca(2+) signaling does not inhibit BFA body formation.

Published
2022

122. Cell wall constrains lateral diffusion of plant plasma-membrane proteins

Author

Martinière, Alexandre, Lavagi, Irene, Nageswaran, Gayathri, Rolfe, Daniel J., Maneta-Peyret, Lilly, Luu, Doan-Trung, Botchway, Stanley W., Webb, Stephen E. D., Mongrand, Sebastien, Maurel, Christophe, Martin-Fernandez, Marisa L., Kleine-Vehn, Jürgen, Friml, Jirí, Moreau, Patrick, and Runions, John

Published
2012

127. Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between brassinosteroid and auxin signaling

Author

Lu, Qing, primary, Zhang, Yonghong, additional, Hellner, Joakim, additional, Giannini, Caterina, additional, Xu, Xiangyu, additional, Pauwels, Jarne, additional, Ma, Qian, additional, Dejonghe, Wim, additional, Han, Huibin, additional, Van de Cotte, Brigitte, additional, Impens, Francis, additional, Gevaert, Kris, additional, De Smet, Ive, additional, Friml, Jiří, additional, Molina, Daniel Martinez, additional, and Russinova, Eugenia, additional

Published
2022
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128. Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components

Author

Dahhan, Dana A, primary, Reynolds, Gregory D, additional, Cárdenas, Jessica J, additional, Eeckhout, Dominique, additional, Johnson, Alexander, additional, Yperman, Klaas, additional, Kaufmann, Walter A, additional, Vang, Nou, additional, Yan, Xu, additional, Hwang, Inhwan, additional, Heese, Antje, additional, De Jaeger, Geert, additional, Friml, Jiří, additional, Van Damme, Daniël, additional, Pan, Jianwei, additional, and Bednarek, Sebastian Y, additional

Published
2022
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136. Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots

Author

Wang, Ren, Himschoot, Ellie, Grenzi, Matteo, Chen, Jian, Safi, Alaeddine, Krebs, Melanie, Schumacher, Karin, Nowack, Moritz, Moeder, Wolfgang, Yoshioka, Keiko, Van Damme, Daniël, De Smet, Ive, Geelen, Danny, Beeckman, Tom, Friml, Jiří, Costa, Alex, and Vanneste, Steffen

Subjects
endosomes, trafficking, Arabidopsis, Biology and Life Sciences, Auxin-analogs, Calcium, PIN protein, root, signaling
Abstract

Much of what we know about the role of auxin in plant development derives from exogenous manipulations of auxin distribution and signaling, using inhibitors, auxins, and auxin analogs. In this context, synthetic auxin analogs, such as 1-naphthalene acetic acid (1-NAA), are often favored over the endogenous auxin, indole-3-acetic acid (IAA), in part due to their higher stability. While such auxin analogs have proven instrumental in revealing the various faces of auxin, they display in some cases bioactivities distinct from IAA. Here, we focused on the effect of auxin analogs on the accumulation of PIN proteins in brefeldin A-sensitive endosomal aggregations (BFA bodies), and correlation with the ability to elicit Ca2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin analog-induced Ca2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited a Ca2+ response, and their differential ability to elicit Ca2+ responses correlated partially with their ability to inhibit BFA-body formation. However, in tir1/afb and cngc14, 1-NAA-induced Ca2+ signaling was strongly impaired, yet 1-NAA still could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent Ca2+ signaling does not inhibit BFA body formation in Arabidopsis roots. Synthetic auxin analog-induced Ca(2+)signaling is independent of inhibition of PIN accumulation in brefeldin A-sensitive endosomal aggregations.

Published
2022

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