774 results on '"Fakin A"'
Search Results
102. SPODBUJANJE KRITIČNEGA MIŠLJENJA PREK VSEBIN KULTURNE DEDIŠČINE.
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Bajec, Jasna Fakin, Bratož, Silva, Štemberger, Tina, and Bajec, Melita Lemut
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YOUNG adults ,PARTICIPANT observation ,CRITICAL thinking ,CULTURAL property ,PARTICIPATION ,DISCOURSE - Abstract
Copyright of Glasnik Slovenskega Etnološkega Društva is the property of Slovene Ethnological Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2023
103. Clinical and electrophysiology findings in Slovene patients with Leber hereditary optic neuropathy
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Jarc-Vidmar, Martina, Tajnik, Mojca, Brecelj, Jelka, Fakin, Ana, Sustar, Maja, Naji, Mateja, Stirn-Kranjc, Branka, Glavač, Damjan, and Hawlina, Marko
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- 2015
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104. Adsorption of Pollutants from Colored Wastewaters after Natural Wool Dyeing
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Ojstršek, Alenka, primary, Vouk, Primož, additional, and Fakin, Darinka, additional
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- 2022
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105. The Role of Vitamin A in Retinal Diseases
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Sajovic, Jana, primary, Meglič, Andrej, additional, Glavač, Damjan, additional, Markelj, Špela, additional, Hawlina, Marko, additional, and Fakin, Ana, additional
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- 2022
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106. Dediščina v akciji
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Fakin Bajec, Jasna, primary, Pogačar, Martin, primary, Štraus, Matevž, primary, and Lemut Bajec, Melita, primary
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- 2022
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107. Fabric hand of a dry finished wool fabric
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Jevšnik, Simona, Kalaoğlu, Fatma, Saricam, Canan, Eryuruk, Selin Hanife, Bahadir, Senem Kursun, Fakin, Darinka, and Zoran, Stjepanovič
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- 2014
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108. Detection and Perception of Colour Regarding Gender and Age
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Design, Smetanova , Maribor, Slovenia, Darinka Fakin, Lavra Smoljanović, and Alenka Ojstršek
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Polymers and Plastics ,genetic structures ,media_common.quotation_subject ,colour perception ,colour of sleeping area ,General Business, Management and Accounting ,Industrial and Manufacturing Engineering ,Developmental psychology ,Age and gender ,colour popularity ,lcsh:TP890-933 ,Perception ,survey ,colour of joy ,lcsh:Textile bleaching, dyeing, printing, etc ,Business and International Management ,Psychology ,colour of clothes ,media_common - Abstract
People have been accompanied by colours throughout the history and through all periods of life. In different eras, colours were also associated with status symbols of different social classes or mythological beliefs. We are often addressed emotionally by colour combinations, since colour perception is always and exclusively a sensorial experience. Various colour combinations can have a pleasant effect on us or leave us cold as well as in a state of shock. All of the above, presented the starting point of this research. Detection and perception of sensations through colour was accomplished by preparing a questionnaire related to 12 selected colours. The research included 302 participants from Slovenia, of both sexes and different ages, born from 1940 to 2004. From the obtained results, it could be concluded how popular a certain colour is in a material, design and spiritual sense. Moreover, the results were validated and compared regarding the gender and age of participants, and further compared with the results of previous studies.
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- 2020
109. The interpretation and utilization of cultural heritage and Its values by young people in Slovenia
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Jasna Fakin Bajec
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Cultural Studies ,Cultural heritage ,heritage values ,young people ,educational curriculum ,voluntary activities ,neoliberal society ,Slovenia ,Anthropology ,Ethnography ,baštinske vrijednosti ,mladi ,obrazovni kurikul ,volonterske aktivnosti ,neoliberalno društvo ,Slovenija ,Environmental ethics ,Sociology ,Valuation (finance) - Abstract
This paper analyses approaches to involving young people in the processes of valuation, interpretation and utilization of local heritage to meet contemporary development challenges. The starting point is the finding that European development strategies highlight the various potentials of cultural heritage, but young people from western Slovenia hardly see any of these potentials. Moreover, the presented ethnographic research results show that young people are not very interested in heritage practices undertaken in their cities or villages. This paper’s results demonstrate how heritage may be introduced to young generations, how youth understand local heritage and its values, and what they think about voluntary activities in cultural fields., U radu se analiziraju pristupi uključivanja mladih u procese vrednovanja, interpretacije i korištenja lokalne baštine ususret suvremenim izazovima razvoja. Polazi se od toga da europske razvojne strategije naglašavaju različite potencijale kulturne baštine, no mladi iz zapadne Slovenije teško uviđaju te potencijale. Nadalje, rezultati etnografskog istraživanja pokazuju da mladi nisu značajnije zainteresirani za prakse vezane uz baštinu koje se provode u njihovim gradovima i selima. Rezultati ovog rada pokazuju na koji se način baština može predstaviti mlađim generacijama, kako mladi razumiju lokalnu baštinu i njezine vrijednosti te što misle o volonterskim aktivnostima u području kulture.
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- 2019
110. Natural Dyeing of Wool Using Junglans regia (Common Walnut) Leaf Extract
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Darinka Fakin, Design, Smetanova , Maribor, Slovenia, and Alenka Ojstršek
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natural colourants ,antimicrobial activity ,Polymers and Plastics ,Chemistry ,ferrous sulphate ,colourimetry ,Pulp and paper industry ,General Business, Management and Accounting ,Industrial and Manufacturing Engineering ,Natural (archaeology) ,lcsh:TP890-933 ,Wool ,wool dyeing ,lcsh:Textile bleaching, dyeing, printing, etc ,Business and International Management ,Dyeing ,walnut leaf extract - Abstract
The main objective of the presented research was to study the possibility of using natural colourants obtained through the aqueous extraction of fresh leaves from the Junglans regia (J. regia), or common walnut tree, for the dyeing of wool yarn. A unique principle was explored by combining the phases of extraction and mordanting into one with the aim of shortening the dyeing procedure, while at the same time extracting more colouring components. Spectrophotometric studies revealed the signifi cant impact of both mordant addition and dye-bath pH on the absorbance curve and thus on the colour and K/S values of the dyed samples. A meta-ordanting technique using ferrous sulphate produced a greater depth of shade at a wavelength of 400 nm, with respect to the concentration of mordant and the liquid ratio of the extraction. Finally, wool yarn dyed with pure leaf extracts exhibited a potent inhibiting activity against Candida albicans (C. albicans) with a moderate reduction rate of 59%, and an inhibited response against Staphylococcus aureus (S. aureus) with a low reduction rate of 38.6%.
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- 2019
111. A novel hotspot of gelsolin instability triggers an alternative mechanism of amyloid aggregation
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Marco Gobbi, Carmina Natale, Luisa Diomede, Rebecca Alemani, Mario Milani, Alberto Barbiroli, Michela Bollati, Patrizia Cioni, Irene Boniardi, Toni Giorgino, Eloise Mastrangelo, Gianluca Presciuttini, Ana Fakin, Marten Beeg, Matteo de Rosa, Edi Gabellieri, and Elisa Fagnani
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Mutant ,Biophysics ,Cleavage (embryo) ,Biochemistry ,Pathogenic variant ,Structural Biology ,Genetics ,medicine ,GSN, gelsolin ,Furin ,Gelsolin ,ComputingMethodologies_COMPUTERGRAPHICS ,Misfolding ,biology ,Chemistry ,AGel, gelsolin amyloidosis ,Amyloidosis ,Dystrophy ,medicine.disease ,Computer Science Applications ,Cell biology ,Proteotoxicity ,biology.protein ,C. elegans ,TP248.13-248.65 ,Cutis laxa ,Biotechnology ,Research Article - Abstract
Graphical abstract, Highlights • Three novel gelsolin amyloidogenic substitutions cluster at the G4:G5 interface. • Mutations impair stability through strand distortion, charge and steric repulsion. • Mutations do not increase sensitivity to furin, the first step of the canonical pathway. • In the unproteolysed form these variants tend to aggregate and are proteotoxic. • The variants aggregate via an alternative, likely proteolysis-independent, mechanism., Gelsolin comprises six homologous domains, named G1 to G6. Single point substitutions in this protein are responsible for AGel amyloidosis, a hereditary disease causing progressive corneal lattice dystrophy, cutis laxa, and polyneuropathy. Although several different amyloidogenic variants of gelsolin have been identified, only the most common mutants present in the G2 domain have been thoroughly characterized, leading to clarification of the functional mechanism. The molecular events underlying the pathological aggregation of 3 recently identified mutations, namely A551P, E553K and M517R, all localized at the interface between G4 and G5, are here explored for the first time. Structural studies point to destabilization of the interface between G4 and G5 due to three structural determinants: β-strand breaking, steric hindrance and/or charge repulsion, all implying impairment of interdomain contacts. Such rearrangements decrease the temperature and pressure stability of gelsolin but do not alter its susceptibility to furin cleavage, the first event in the canonical aggregation pathway. These variants also have a greater tendency to aggregate in the unproteolysed forms and exhibit higher proteotoxicity in a C. elegans-based assay. Our data suggest that aggregation of G4G5 variants follows an alternative, likely proteolysis-independent, pathway.
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- 2021
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112. Reduction of Lead and Antimony Ions from the Crystal Glass Wastewaters Utilising Adsorption
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Darinka Fakin, Natalija Gorjanc, and Alenka Ojstršek
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Flocculation ,adsorbents ,antimony ,Geography, Planning and Development ,Inorganic chemistry ,TJ807-830 ,chemistry.chemical_element ,Management, Monitoring, Policy and Law ,TD194-195 ,Renewable energy sources ,Adsorption ,Antimony ,medicine ,GE1-350 ,Zeolite ,lead ,Environmental effects of industries and plants ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Environmental sciences ,wastewater treatment ,Lead glass ,Wastewater ,visual_art ,visual_art.visual_art_medium ,Sewage treatment ,crystal glass industry ,Activated carbon ,medicine.drug - Abstract
The presented research examined five adsorbents, i.e., zeolite 4A, a mixture of three zeolites (4A, 13X, and ZSM-5), natural zeolite (tuff), activated carbon, and peat, and their potential capability for removal of exceeded ions of lead (Pb), antimony (Sb), sulphates (SO42−), and fluorides (F−) from real wastewater generated in the crystal glass industry, which was previously treated in-situ by flocculation, with the aim to attain the statutory values for discharge into watercourses or possible recycling. The screening experiment evidenced that the tuff was the most suitable adsorbent for the reduction of Pb (93.8%) and F− (98.1%). It also lowered wastewater’s pH sufficiently from 9.6 to 7.8, although it was less appropriate for the reduction of Sb (66.7%) as compared to activated carbon (96.7%) or peat (99.9%). By adjusting the pH of the initial wastewater to pH 5, its adsorption capacity even enlarged. Results from the tuff-filled column experiment revealed reduction of Pb up to 97%, Sb up to 80%, and F− up to 96%, depending on the velocity flow, and thus it could be used for post-treatment (and recycling) of wastewaters from the crystal glass industry. Moreover, the system showed an explicit buffering capacity, but negligible reduction of the SO42−.
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- 2021
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113. Thermal, Mechanical and Optical Features of Aluminosilicate-Coated Cotton Textiles via the Crosslinking Method
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Alenka Ojstršek, Silvo Hribernik, and Darinka Fakin
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zeolites ,cotton ,pad-dry-thermofix ,crosslinking agent ,functional properties ,Organic chemistry ,QD241-441 - Abstract
The presented study focuses on the development of a pad-dry-thermofix functional coating process using a mixture of microporous aluminosilicate particles in diverse bath formulations to impart UV-ray-blocking, thermal stability and easy-care properties to the cotton fabric. The results of Scanning Electron Microscopy (SEM) and X-ray powder Diffraction (XRD) revealed the presence of three different types of zeolites within the examined sample, i.e., the largest amount being zeolite A, followed by the zeolite X, and the zeolite ZSM-5. The surface characterization results of zeolite-coated/cross-linked textiles provided evidence of acceptable UV-ray-blocking properties and increased thermal stability, as well as enhanced tensile strength and breaking tenacity without considerably decreasing the whiteness degree. Moreover, the dry crease recovery angle increased for the cotton fabric cross-linked via an mDMDHEU, and decreased significantly using 30 g/L zeolites negatively influencing qualitative values. TG/DTA results have proven the enlarged thermal stability of aluminosilicate-coated cotton, although combustion was not prevented.
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- 2018
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114. LorisQ – Fully digital solution for the equipment maintanance woes (DCCS before DCCS)
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Prugovecki, Sinisa, primary, Fakin, Mirna, additional, and Lerotic, Mirna, additional
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- 2021
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115. Reduction of Lead and Antimony Ions from the Crystal Glass Wastewaters Utilising Adsorption
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Ojstršek, Alenka, primary, Gorjanc, Natalija, additional, and Fakin, Darinka, additional
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- 2021
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116. Characteristics of Retinitis Pigmentosa Associated with ADGRV1 and Comparison with USH2A in Patients from a Multicentric Usher Syndrome Study Treatrush
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José-Alain Sahel, Saddek Mohand-Said, Isabelle Audo, Crystel Bonnet, Eberhart Zrenner, Marko Hawlina, Anne Kurtenbach, Francesca Simonelli, Ditta Zobor, Christine Petit, Katarina Stingl, Francesco Testa, Ana Fakin, University Medical Centre Ljubljana [Ljubljana, Slovenia] (UMCL), Institut de l'Audition [Paris] (IDA), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Université Paris Cité (UPCité), Eberhard Karls Universität Tübingen = Eberhard Karls University of Tuebingen, Institut de la Vision, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO), Università degli studi della Campania 'Luigi Vanvitelli' = University of the Study of Campania Luigi Vanvitelli, Hôpital de la Fondation Ophtalmologique Adolphe de Rothschild [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), University of Pittsburgh School of Medicine, Pennsylvania Commonwealth System of Higher Education (PCSHE), Collège de France (CdF (institution)), This work was supported by the European Union Seventh Framework Programme under the grant agreement HEALTH-F2-2010-242013 (TREATRUSH), the Slovenian Research Agency (grant ARRS J3-1750), and the Tistou and Charlotte Kerstan Foundation, Tübingen., European Project: 242013,EC:FP7:HEALTH,FP7-HEALTH-2009-single-stage,TREATRUSH(2010), HAL-SU, Gestionnaire, Fighting blindness of Usher syndrome: diagnosis, pathogenesis and retinal treatment (TreatRetUsher) - TREATRUSH - - EC:FP7:HEALTH2010-02-01 - 2014-01-31 - 242013 - VALID, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Paris (UP), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Università degli studi della Campania 'Luigi Vanvitelli', and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
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Retinal degeneration ,Male ,Visual acuity ,genetic structures ,Usher syndrome ,Receptors, G-Protein-Coupled ,USH2A ,0302 clinical medicine ,Loss of Function Mutation ,Biology (General) ,10. No inequality ,Child ,Spectroscopy ,Aged, 80 and over ,0303 health sciences ,Extracellular Matrix Proteins ,[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology ,fundus autofluorescence ,hyperautofluorescent ring ,VLGR1 ,General Medicine ,Middle Aged ,Computer Science Applications ,Chemistry ,Exact test ,Child, Preschool ,Usher syndrome (USH) ,Female ,medicine.symptom ,Usher Syndromes ,MASS1 ,Tomography, Optical Coherence ,Retinopathy ,Adult ,medicine.medical_specialty ,GPR98 ,Adolescent ,QH301-705.5 ,Catalysis ,Nyctalopia ,Article ,ADGRV1 ,Inorganic Chemistry ,03 medical and health sciences ,Ophthalmology ,retinitis pigmentosa ,Retinitis pigmentosa ,otorhinolaryngologic diseases ,medicine ,Humans ,Physical and Theoretical Chemistry ,QD1-999 ,Molecular Biology ,Macular edema ,030304 developmental biology ,Aged ,adhesion G protein-coupled receptor V1 ,business.industry ,Organic Chemistry ,Infant ,medicine.disease ,eye diseases ,030221 ophthalmology & optometry ,business ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
In contrast to USH2A, variants in ADGRV1 are a minor cause of Usher syndrome type 2, and the associated phenotype is less known. The purpose of the study was to characterize the retinal phenotype of 18 ADGRV1 patients (9 male, 9 female, median age 52 years) and compare it with that of 204 USH2A patients (111 male, 93 female, median age 43 years) in terms of nyctalopia onset, best corrected visual acuity (BCVA), fundus autofluorescence (FAF), and optical coherence tomography (OCT) features. There was no statistical difference in the median age at onset (30 and 18 years, Mann–Whitney U test, p = 0.13), the mean age when 50% of the patients reached legal blindness (≥1.0 log MAR) based on visual acuity (64 years for both groups, log-rank, p = 0.3), the risk of developing advanced retinal degeneration (patch or atrophy) with age (multiple logistic regression, p = 0.8), or the frequency of cystoid macular edema (31% vs. 26%, Fisher’s exact test, p = 0.4). ADGRV1 and USH2A retinopathy were indistinguishable in all major functional and structural characteristics, suggesting that the loss of function of the corresponding proteins produces similar effects in the retina. The results are important for counseling ADGRV1 patients, who represent the minor patient subgroup.
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- 2021
117. Disease Progression in CNGA3 and CNGB3 Retinopathy
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Pompe, Manca Tekavčič, Vrabič, Nika, Volk, Marija, Meglič, Andrej, Jarc-Vidmar, Martina, Peterlin, Borut, Hawlina, Marko, and Fakin, Ana
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disease stage ,optical coherence tomography ,genetic structures ,FAF ,degeneration ,autofluorescence ,eye diseases ,CNGA3 ,cone-dystrophy ,color vision ,OCT ,ERG ,CNGB3 ,foveal hypoplasia ,sense organs ,progression ,achromatopsia ,electroretinography ,nystagmus - Abstract
Achromatopsia has been proposed to be a morphologically predominately stable retinopathy with rare reports of progression of structural changes in the macula. A five-grade system of optical coherence tomography (OCT) features has been used for the classification of structural macular changes. However, their association with age remains questionable. We characterized the Slovenian cohort of 12 patients with pathogenic variants in CNGA3 or CNGB3 who had been followed up with OCT for up to 9 years. Based on observed structural changes in association with age, the following four-stage classification of retinal morphological changes was proposed: (I) preserved inner segment ellipsoid band (Ise), (II) disrupted ISe, (III) ISe loss and (IV) ISe and RPE loss. Data from six previously published studies reporting OCT morphology in CNGA3 and CNGB3 patients were additionally collected, forming the largest CNGA3/CNGB3 cohort to date, comprising 126 patients aged 1–71 years. Multiple regression analysis showed a significant correlation of OCT stage with age (p <, 0.001) and no correlation with gene (p >, 0.05). The median ages of patients with stages I–IV were 12 years, 23 years, 27 years and 48 years, respectively, and no patient older than 50 years had continuous ISe. Our findings suggest that achromatopsia presents with slowly but steadily progressive structural changes of the macular outer retinal layers. However, whether morphological changes in time follow the proposed four-stage linear pattern needs to be confirmed in a long-term study.
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- 2021
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118. Luxury food tour: Perspectives and dilemmas on the 'luxurification' of local culture in tourism product
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Jasna Fakin Bajec and Saša Poljak Istenič
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Local culture ,business.industry ,media_common.quotation_subject ,05 social sciences ,Geography, Planning and Development ,Face (sociological concept) ,Gastronomy ,Public relations ,Experiential learning ,Perception ,0502 economics and business ,Mediation ,General Earth and Planetary Sciences ,050211 marketing ,Sociology ,Product (category theory) ,business ,050212 sport, leisure & tourism ,Tourism ,media_common - Abstract
This paper focuses on the Kras/Carso Food Tour to present the challenges of developing a luxury tourism product. The tour's design followed the current strategy of the Slovenian Tourist Board, which defines specific criteria for a luxury experience. Contextualized by the experiential trends in tourism, the paper juxtaposes the bottom-up and top-down perspectives on luxury experiences. The authors argue that mediation by experts familiar with academic discourses and local culture is beneficial for the development of a successful tourism product. The study contributes to the debates on luxury tourism, which have neglected bottom-up perceptions in tourist discourse and overlooked the dilemmas people face when "luxurifying" their traditions and heritage practices.
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- 2021
119. Disease Progression in
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Manca, Tekavčič Pompe, Nika, Vrabič, Marija, Volk, Andrej, Meglič, Martina, Jarc-Vidmar, Borut, Peterlin, Marko, Hawlina, and Ana, Fakin
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Adult ,Male ,Adolescent ,Slovenia ,Age Factors ,Cyclic Nucleotide-Gated Cation Channels ,Infant ,Color Vision Defects ,Middle Aged ,Cohort Studies ,Young Adult ,Retinal Diseases ,Child, Preschool ,Mutation ,Disease Progression ,Humans ,Female ,Genetic Predisposition to Disease ,Longitudinal Studies ,Child ,Tomography, Optical Coherence ,Aged - Abstract
Achromatopsia has been proposed to be a morphologically predominately stable retinopathy with rare reports of progression of structural changes in the macula. A five-grade system of optical coherence tomography (OCT) features has been used for the classification of structural macular changes. However, their association with age remains questionable. We characterized the Slovenian cohort of 12 patients with pathogenic variants in
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- 2021
120. Resolving the dark matter of ABCA4 for 1054 Stargardt disease probands through integrated genomics and transcriptomics
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Ymkje M. Hettinga, Karsten Hufendiek, Jacek P. Szaflik, Ian M. MacDonald, Isabelle Meunier, Marcela D. Mena, Kaoru Fujinami, Mubeen Khan, Eyal Banin, Elfride De Baere, G. Jane Farrar, Adrian Dockery, Rianne Miller, Tamar Ben-Yosef, Manar Salameh, L. Ingeborgh van den Born, Anna M Tracewska, Sandro Banfi, Caroline C W Klaver, John N. De Roach, Carmen Ayuso, Sabine Defoort, Damjan Glavač, Ulrich Kellner, Juliana Maria Ferraz Sallum, Claire-Marie Dhaenens, Stéphanie S. Cornelis, Bernhard H. F. Weber, Klaus Rüther, Jennifer A. Thompson, Bernard Puech, Raj Ramesar, Aurore Devos, Lisa Roberts, Herbert Jägle, Osvaldo L. Podhajcer, Hadas Newman, Bohdan Kousal, Femke Bults, Marta Del Pozo-Valero, Marc Pieterse, Laura Whelan, Xavier Zanlonghi, Alaa AlTalbishi, Francesca Simonelli, Marloes Steehouwer, Caroline Thuillier, Frans P.M. Cremers, Andrea L Vincent, Smaragda Kamakari, Ana Fakin, Anna Matynia, Dror Sharon, Ketan Mishra, Mariana Vallim Salles, Heidi Stöhr, Miriam Bauwens, Petra Liskova, Esmee H. Runhart, Buhle Ntozini, Georg Spital, Carel B. Hoyng, Takaaki Hayashi, Terri L. McLaren, Martine van Zweeden, Lubica Dudakova, Camiel J. F. Boon, Christian Gilissen, Jacquie Greenberg, Monika Ołdak, Tina M. Lamey, Yahya AlSwaiti, Alexander Hoischen, Marianthi Karali, Michael B. Gorin, Ophthalmology, ANS - Complex Trait Genetics, Khan, Mubeen, Cornelis, Stéphanie S, Pozo-Valero, Marta Del, Whelan, Laura, Runhart, Esmee H, Mishra, Ketan, Bults, Femke, Alswaiti, Yahya, Altalbishi, Alaa, De Baere, Elfride, Banfi, Sandro, Banin, Eyal, Bauwens, Miriam, Ben-Yosef, Tamar, Boon, Camiel J F, van den Born, L Ingeborgh, Defoort, Sabine, Devos, Aurore, Dockery, Adrian, Dudakova, Lubica, Fakin, Ana, Farrar, G Jane, Sallum, Juliana Maria Ferraz, Fujinami, Kaoru, Gilissen, Christian, Glavač, Damjan, Gorin, Michael B, Greenberg, Jacquie, Hayashi, Takaaki, Hettinga, Ymkje M, Hoischen, Alexander, Hoyng, Carel B, Hufendiek, Karsten, Jägle, Herbert, Kamakari, Smaragda, Karali, Marianthi, Kellner, Ulrich, Klaver, Caroline C W, Kousal, Bohdan, Lamey, Tina M, Macdonald, Ian M, Matynia, Anna, Mclaren, Terri L, Mena, Marcela D, Meunier, Isabelle, Miller, Rianne, Newman, Hada, Ntozini, Buhle, Oldak, Monika, Pieterse, Marc, Podhajcer, Osvaldo L, Puech, Bernard, Ramesar, Raj, Rüther, Klau, Salameh, Manar, Salles, Mariana Vallim, Sharon, Dror, Simonelli, Francesca, Spital, Georg, Steehouwer, Marloe, Szaflik, Jacek P, Thompson, Jennifer A, Thuillier, Caroline, Tracewska, Anna M, van Zweeden, Martine, Vincent, Andrea L, Zanlonghi, Xavier, Liskova, Petra, Stöhr, Heidi, Roach, John N De, Ayuso, Carmen, Roberts, Lisa, Weber, Bernhard H F, Dhaenens, Claire-Marie, and Cremers, Frans P M
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DEEP-INTRONIC VARIANTS ,Proband ,smMIP ,lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] ,ABCA4 ,RPE65 ,Sensory disorders Donders Center for Medical Neuroscience [Radboudumc 12] ,Macular Degeneration ,Exon ,0302 clinical medicine ,Missing heritability problem ,purl.org/becyt/ford/3.2 [https] ,Medicine and Health Sciences ,smMIPs ,MUTATION ,Genetics (clinical) ,Genetics ,variants ,0303 health sciences ,structural ,biology ,Metabolic Disorders Radboud Institute for Molecular Life Sciences [Radboudumc 6] ,Genomics ,DYSTROPHY ,Pedigree ,3. Good health ,Stargardt disease ,MATERNAL UNIPARENTAL ISODISOMY ,purl.org/becyt/ford/3 [https] ,RETINAL ,CHROMOSOME-1 ,PATIENT ,STRUCTURAL VARIANTS ,Deep sequencing ,03 medical and health sciences ,SDG 3 - Good Health and Well-being ,deep-intronic variants ,REVEALS ,medicine ,Humans ,030304 developmental biology ,REPAIR ,deep-intronic variant ,structural variants ,medicine.disease ,GENE ,Introns ,Uniparental Isodisomy ,Mutation ,030221 ophthalmology & optometry ,biology.protein ,ATP-Binding Cassette Transporters ,Transcriptome - Abstract
Purpose: Missing heritability in human diseases represents a major challenge, and this is particularly true for ABCA4-associated Stargardt disease (STGD1). We aimed to elucidate the genomic and transcriptomic variation in 1054 unsolved STGD and STGD-like probands. Methods: Sequencing of the complete 128-kb ABCA4 gene was performed using single-molecule molecular inversion probes (smMIPs), based on a semiautomated and cost-effective method. Structural variants (SVs) were identified using relative read coverage analyses and putative splice defects were studied using in vitro assays. Results: In 448 biallelic probands 14 known and 13 novel deep-intronic variants were found, resulting in pseudoexon (PE) insertions or exon elongations in 105 alleles. Intriguingly, intron 13 variants c.1938-621G>A and c.1938-514G>A resulted in dual PE insertions consisting of the same upstream, but different downstream PEs. The intron 44 variant c.6148-84A>T resulted in two PE insertions and flanking exon deletions. Eleven distinct large deletions were found, two of which contained small inverted segments. Uniparental isodisomy of chromosome 1 was identified in one proband. Conclusion: Deep sequencing of ABCA4 and midigene-based splice assays allowed the identification of SVs and causal deep-intronic variants in 25% of biallelic STGD1 cases, which represents a model study that can be applied to other inherited diseases. Fil: Khan, Mubeen. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Cornelis, Stéphanie S.. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Del Pozo Valero, Marta. Hospital Universitario Fundación Jiménez Díaz; España. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Whelan, Laura. Trinity College; Estados Unidos Fil: Runhart, Esmee H.. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Mishra, Ketan. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Bults, Femke. Radboud University Nijmegen Medical Centre; Países Bajos Fil: AlSwaiti, Yahya. St John of Jerusalem Eye Hospital Group; Palestina (ANP) Fil: AlTalbishi, Alaa. St John of Jerusalem Eye Hospital Group; Palestina (ANP) Fil: De Baere, Elfride. University of Ghent; Bélgica Fil: Banfi, Sandro. Seconda Universita Degli Studi Di Napoli; Italia Fil: Banin, Eyal. The Hebrew University of Jerusalem; Israel Fil: Bauwens, Miriam. University of Ghent; Bélgica Fil: Ben Yosef, Tamar. The Ruth And Bruce Rappaport Faculty Of Medicine; Israel Fil: Boon, Camiel J. F.. Leiden University. Leiden University Medical Center; Países Bajos Fil: van den Born, L. Ingeborgh. Rotterdam Ophthalmic Institute; Países Bajos Fil: Defoort, Sabine. Universite Lille; Francia Fil: Devos, Aurore. Universite Lille; Francia Fil: Dockery, Adrian. Trinity College; Estados Unidos Fil: Dudakova, Lubica. Charles University and General University Hospital; República Checa Fil: Fakin, Ana. Charles University and General University Hospital; República Checa Fil: Farrar, G. Jane. Trinity College; Estados Unidos Fil: Ferraz Sallum, Juliana Maria. Universidade Federal de Sao Paulo; Brasil Fil: Fujinami, Kaoru. UCL Institute of Ophthalmology; Reino Unido Fil: Gilissen, Christian. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Glavac, Damjan. University of Ljubljana; Eslovenia Fil: Gorin, Michael B.. University of California at Los Angeles. School of Medicine; Estados Unidos Fil: Greenberg, Jacquie. University of Cape Town; Sudáfrica Fil: Hayashi, Takaaki. The Jikei University School of Medicine; Japón Fil: Hettinga, Ymkje M.. Bartiméus Diagnostic Center for Complex Visual Disorders; Países Bajos Fil: Hoischen, Alexander. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Hoyng, Carel B.. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Hufendiek, Karsten. University Eye Hospital Hannover Medical School; Alemania Fil: Jägle, Herbert. University Regensburg; Alemania Fil: Kamakari, Smaragda. OMMA Ophthalmological Institute of Athens; Grecia Fil: Karali, Marianthi. Seconda Universita Degli Studi Di Napoli; Italia Fil: Kellner, Ulrich. No especifíca; Fil: Klaver, Caroline C. W.. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Kousal, Bohdan. Charles University and General University Hospital; República Checa Fil: Lamey, Tina M.. University of Western Australia; Australia Fil: MacDonald, Ian M.. University of Alberta; Canadá Fil: Matynia, Anna. University of California at Los Angeles. School of Medicine; Estados Unidos Fil: McLaren, Terri L.. University of Western Australia; Australia Fil: Mena, Marcela D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Meunier, Isabelle. Université Montpellier II; Francia Fil: Miller, Rianne. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Newman, Hadas. Universitat Tel Aviv; Israel Fil: Ntozini, Buhle. University of Cape Town; Sudáfrica Fil: Oldak, Monika. No especifíca; Fil: Pieterse, Marc. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Podhajcer, Osvaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Puech, Bernard. Universite Lille; Francia Fil: Ramesar, Raj. University of Cape Town; Sudáfrica Fil: Rüther, Klaus. No especifíca; Fil: Salameh, Manar. No especifíca; Fil: Salles, Mariana Vallim. Universidade de Sao Paulo; Brasil Fil: Sharon, Dror. The Hebrew University of Jerusalem; Israel Fil: Simonelli, Francesca. Seconda Universita Degli Studi Di Napoli; Italia Fil: Spital, Georg. No especifíca; Fil: Steehouwer, Marloes. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Szaflik, Jacek P.. No especifíca; Fil: Thompson, Jennifer A.. No especifíca; Fil: Thuillier, Caroline. Universite Lille; Francia Fil: Tracewska, Anna M.. No especifíca; Fil: van Zweeden, Martine. Radboud University Nijmegen Medical Centre; Países Bajos Fil: Vincent, Andrea L.. University of Auckland; Nueva Zelanda Fil: Zanlonghi, Xavier. No especifíca; Fil: Liskova, Petra. Charles University and General University Hospital; República Checa Fil: Stöhr, Heidi. Universitat Regensburg; Alemania Fil: De Roach, John N.. University of Western Australia; Australia Fil: Ayuso, Carmen. Hospital Universitario Fundación Jiménez Díaz; España Fil: Roberts, Lisa. University of Cape Town; Sudáfrica Fil: Weber, Bernhard H. F.. Universitat Regensburg; Alemania Fil: Dhaenens, Claire Marie. Universite Lille; Francia Fil: Cremers, Frans P. M.. Radboud University Nijmegen Medical Centre; Países Bajos
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- 2020
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121. Aspectos gerais e número de etapas do sistema de medicação de quatro hospitais brasileiros Aspectos generales y número de etapas del sistema de medicación en cuatro hospitales brasileños General aspects and number of phases of the medication system in four brazilian hospitals
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Silvia Helena de Bortoli Cassiani, Adriana Inocenti Miasso, Ana Elisa Bauer de Camargo Silva, Flávio Trevisan Fakin, and Regina Célia de Oliveira
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errores de medicación ,sistemas de medicación ,administración hospitalaria ,erros de medicação ,sistemas de medicação ,administração hospitalar ,medication errors ,medication systems ,hospital administration ,Nursing ,RT1-120 - Abstract
Essa investigação identificou e analisou o sistema de medicação de 04 hospitais universitários, localizados nas cidades de Recife, Ribeirão Preto, Goiânia e São Paulo, após a aprovação nos Comitês de Ética em Pesquisa e da autorização da direção dos hospitais. Os dados foram coletados através de entrevista estruturada com um dos profissionais responsáveis pelo sistema de medicação e observação não participante e direta, por uma semana, nos vários sub-sistemas. Os resultados indicaram pontos que necessitam de aperfeiçoamentos como utilização de abreviações, falta de padronização de horários de administração de medicamentos, falta de informações atualizadas e completas do paciente, farmácia não funcionando 24 horas em um hospital, falta de centro de informações de medicamentos e outros. Evidenciou-se no hospital A 66 etapas, no B 58 etapas, no C 70 etapas e no D 80 etapas do sistema de medicação. Simplificar os processos, diminuindo o número de etapas, é a chave para a redução de erros de medicação.Esa investigación identificó y analizó el sistema de medicación en 04 hospitales universitarios en las ciudades de Recife, Ribeirão Preto, Goiânia y São Paulo, Brasil. Los datos fueron recopilados en dos etapas. En la primera etapa se realizó una entrevista estructurada con uno de los profesionales responsables por el sistema de medicación. En la segunda, se hizo observación no participativa y directa durante una semana. Los resultados indicaron los puntos que necesitan ser perfeccionados, tales como uso de abreviaciones, la falta de un padrón de horarios, falta de información actualizada y completa del paciente, farmacia que no funciona las 24 horas en un hospital, y otros. Se observó en el Hospital A 66 etapas, en el B 58 etapas, en el C 70 etapas y en el D 80 etapas respecto al sistema de medicación. Simplificar el proceso, diminuyendo el número de etapas, es la clave para reducir los márgenes de errores en la medicación.This study identified and analyzed the medication systems in 04 university hospitals located in Recife, Ribeirão Preto, Goiânia and São Paulo, Brazil, after approval by the Research Ethics Committee and authorization by the hospital directors. Data were collected through a structured interview with one of the professionals in charge of the medication system and non-participant and direct observation during one week. The results indicated the points requiring improvement, such as the use of abbreviations, lack of standardization in medication administration times, lack of updated and complete information about the patient, the pharmacy's not working 24 hours a day in hospitals and others. 66 phases were shown in Hospital A, 58 in B, 70 in C, and 80 in D concerning the medication system. Simplifying the processes by reducing the number of phases is the key to reducing medication errors.
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- 2004
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122. Characteristics of Retinitis Pigmentosa Associated with ADGRV1 and Comparison with USH2A in Patients from a Multicentric Usher Syndrome Study Treatrush
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Fakin, Ana, primary, Bonnet, Crystel, additional, Kurtenbach, Anne, additional, Mohand-Said, Saddek, additional, Zobor, Ditta, additional, Stingl, Katarina, additional, Testa, Francesco, additional, Simonelli, Francesca, additional, Sahel, José-Alain, additional, Audo, Isabelle, additional, Zrenner, Eberhart, additional, Hawlina, Marko, additional, and Petit, Christine, additional
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- 2021
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123. Disease Progression in CNGA3 and CNGB3 Retinopathy; Characteristics of Slovenian Cohort and Proposed OCT Staging Based on Pooled Data from 126 Patients from 7 Studies
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Tekavčič Pompe, Manca, primary, Vrabič, Nika, additional, Volk, Marija, additional, Meglič, Andrej, additional, Jarc-Vidmar, Martina, additional, Peterlin, Borut, additional, Hawlina, Marko, additional, and Fakin, Ana, additional
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- 2021
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124. Luxury food tour: Perspectives and dilemmas on the "luxurification" of local culture in tourism product
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Poljak Istenič, Saša, primary and Fakin Bajec, Jasna, additional
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- 2021
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125. ABCA4-associated disease as a model for missing heritability in autosomal recessive disorders: novel noncoding splice, cis-regulatory, structural, and recurrent hypomorphic variants
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Bernd Wissinger, Thomy de Ravel de l'Argentière, Frans P.M. Cremers, Jim Bauwens, Bart P. Leroy, Riccardo Sangermano, Caroline Van Cauwenbergh, Julie De Zaeytijd, Ana Fakin, Sarah De Jaegere, Toon Rosseel, Mubeen Khan, Gavin Arno, Susanne Kohl, Andrew R. Webster, Meindert De Vries, Elfride De Baere, Rob W.J. Collin, Alejandro Garanto, Irina Balikova, Keren J. Carss, Thalia Van Laethem, Miriam Bauwens, Kim De Leeneer, Marnik Vuylsteke, Sarah Naessens, Yves Sznajer, Timothy J. Cherry, Françoise Sadler, Nicole Weisschuh, Software Languages Lab, Informatics and Applied Informatics, Faculty of Sciences and Bioengineering Sciences, UCL - (SLuc) Centre de génétique médicale UCL, and UCL - SSS/IREC/SLUC - Pôle St.-Luc
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DEEP-INTRONIC VARIANTS ,Male ,ABCA4 ,PHENOTYPE ,Sensory disorders Donders Center for Medical Neuroscience [Radboudumc 12] ,Cohort Studies ,0302 clinical medicine ,Gene Frequency ,Missing heritability problem ,STARGARDT-DISEASE ,Medicine and Health Sciences ,Genetics(clinical) ,Genetics (clinical) ,Genetics ,0303 health sciences ,biology ,noncoding ,deep-intronic ,Exons ,DYSTROPHY ,Middle Aged ,Phenotype ,3. Good health ,Pedigree ,Female ,Adult ,Genes, Recessive ,ANTISENSE OLIGONUCLEOTIDES ,Article ,03 medical and health sciences ,All institutes and research themes of the Radboud University Medical Center ,AON ,Retinitis pigmentosa ,RETINITIS-PIGMENTOSA ,REVEALS ,Retinal Dystrophies ,medicine ,non-coding ,Humans ,splice ,Allele ,Gene ,Alleles ,030304 developmental biology ,SPECTRUM ,TRANSPORTER GENE ABCR ,MUTATIONS ,Biology and Life Sciences ,ABCA4-associated disease ,Oligonucleotides, Antisense ,medicine.disease ,Introns ,Stargardt disease ,HEK293 Cells ,missing heritability ,Mutation ,030221 ophthalmology & optometry ,biology.protein ,ATP-Binding Cassette Transporters - Abstract
PURPOSE: ABCA4-associated disease, a recessive retinal dystrophy, is hallmarked by a large proportion of patients with only one pathogenic ABCA4 variant, suggestive for missing heritability. METHODS: By locus-specific analysis of ABCA4, combined with extensive functional studies, we aimed to unravel the missing alleles in a cohort of 67 patients (p), with one (p = 64) or no (p = 3) identified coding pathogenic variants of ABCA4. RESULTS: We identified eight pathogenic (deep-)intronic ABCA4 splice variants, of which five are novel and six structural variants, four of which are novel, including two duplications. Together, these variants account for the missing alleles in 40.3% of patients. Furthermore, two novel variants with a putative cis-regulatory effect were identified. The common hypomorphic variant c.5603A>T p.(Asn1868Ile) was found as a candidate second allele in 43.3% of patients. Overall, we have elucidated the missing heritability in 83.6% of our cohort. In addition, we successfully rescued three deep-intronic variants using antisense oligonucleotide (AON)-mediated treatment in HEK 293-T cells and in patient-derived fibroblast cells. CONCLUSION: Noncoding pathogenic variants, novel structural variants, and a common hypomorphic allele of the ABCA4 gene explain the majority of unsolved cases with ABCA4-associated disease, rendering this retinopathy a model for missing heritability in autosomal recessive disorders. ispartof: GENETICS IN MEDICINE vol:21 issue:8 pages:1761-1771 ispartof: location:United States status: published
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- 2019
126. Deep-intronic ABCA4 variants explain missing heritability in Stargardt disease and allow correction of splice defects by antisense oligonucleotides
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Gavin Arno, Bernhard H. F. Weber, Carel B. Hoyng, L. Ingeborgh van den Born, Nathalie M. Bax, Silvia Albert, Frans P.M. Cremers, Keren J. Carss, Stéphanie S. Cornelis, Felix Grassmann, Caroline C W Klaver, F. Lucy Raymond, Mubeen Khan, Ana Fakin, Andrew R. Webster, Muhammad Imran Khan, Claire Marie Dhaenens, Riccardo Sangermano, Elfride De Baere, Sarah Naessens, Heidi Stöhr, Rob W.J. Collin, Alberta A H J Thiadens, Jan Willem R. Pott, Esmee H. Runhart, Miriam Bauwens, Bernard Puech, Isabelle Meunier, Joke B. G. M. Verheij, Alejandro Garanto, Ophthalmology, and Epidemiology
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0301 basic medicine ,antisense oligonucleotide ,030105 genetics & heredity ,ABCA4 ,Exon ,Missing heritability problem ,Medicine and Health Sciences ,Protein Isoforms ,Genetics(clinical) ,Child ,Genetics (clinical) ,Exome sequencing ,POPULATION ,Genetics ,education.field_of_study ,Exons ,Middle Aged ,3. Good health ,Pedigree ,Stargardt disease ,RNA splicing ,Adult ,Adolescent ,RNA Splicing ,Population ,Biology ,Polymorphism, Single Nucleotide ,Article ,03 medical and health sciences ,Young Adult ,REVEALS ,medicine ,Humans ,splice ,education ,Gene ,Aged ,MUTATIONS ,deep-intronic variant ,Biology and Life Sciences ,IN-VITRO ,Oligonucleotides, Antisense ,medicine.disease ,GENE ,Introns ,030104 developmental biology ,HEK293 Cells ,Mutation ,missing heritability ,ATP-Binding Cassette Transporters - Abstract
Purpose: Using exome sequencing, the underlying variants in many persons with autosomal recessive diseases remain undetected. We explored autosomal recessive Stargardt disease (STGD1) as a model to identify the missing heritability.Methods: Sequencing of ABCA4 was performed in 8 STGD1 cases with one variant and p.Asn1868Ile in trans, 25 cases with one variant, and 3 cases with no ABCA4 variant. The effect of intronic variants was analyzed using in vitro splice assays in HEK293T cells and patient-derived fibroblasts. Antisense oligonucleotides were used to correct splice defects.Results: In 24 of the probands (67%), one known and five novel deep-intronic variants were found. The five novel variants resulted in messenger RNA pseudoexon inclusions, due to strengthening of cryptic splice sites or by disrupting a splicing silencer motif. Variant c.769-784C>T showed partial insertion of a pseudoexon and was found in cis with c.5603A>T (p.Asn1868Ile), so its causal role could not be fully established. Variant c.4253+43G>A resulted in partial skipping of exon 28. Remarkably, antisense oligonucleotides targeting the aberrant splice processes resulted in (partial) correction of all splicing defects.Conclusion: Our data demonstrate the importance of assessing noncoding variants in genetic diseases, and show the great potential of splice modulation therapy for deep-intronic variants.
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- 2019
127. Stationary and Progressive Phenotypes Caused by the p.G90D Mutation in Rhodopsin Gene
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Marija Volk, Borut Peterlin, Nina Kobal, Marko Hawlina, Tjaša Krašovec, Maja Sustar, and Ana Fakin
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0301 basic medicine ,Retinal degeneration ,Male ,genetic structures ,lcsh:Chemistry ,0302 clinical medicine ,Medicine ,Child ,CSNB ,lcsh:QH301-705.5 ,Spectroscopy ,Congenital stationary night blindness ,congenital stationary night blindness ,sector retinitis pigmentosa ,biology ,medicine.diagnostic_test ,fundus autofluorescence ,RHO ,General Medicine ,Middle Aged ,Computer Science Applications ,Pedigree ,inherited retinal dystrophy ,Phenotype ,Rhodopsin ,ERG ,Female ,medicine.symptom ,Erg ,Adult ,medicine.medical_specialty ,RP ,Adolescent ,Fundus Oculi ,Article ,Catalysis ,Nyctalopia ,Inorganic Chemistry ,03 medical and health sciences ,Young Adult ,constitutively active mutation ,Ophthalmology ,retinitis pigmentosa ,Retinitis pigmentosa ,Humans ,Genetic Predisposition to Disease ,Physical and Theoretical Chemistry ,G90D ,Molecular Biology ,Macular edema ,Aged ,business.industry ,Organic Chemistry ,FAF ,medicine.disease ,eye diseases ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,rhodopsin ,OCT ,Mutation ,030221 ophthalmology & optometry ,biology.protein ,retinal degeneration ,pericentral retinitis pigmentosa ,electroretinography ,business ,Electroretinography - Abstract
Mutations in rhodopsin gene (RHO) are a frequent cause of retinitis pigmentosa (RP) and less often, congenital stationary night blindness (CSNB). Mutation p.G90D has previously been associated with CSNB based on the examination of one family. This study screened 60 patients. Out of these 60 patients, 32 were affected and a full characterization was conducted in 15 patients. We described the clinical characteristics of these 15 patients (12 male, median age 42 years, range 8–71) from three families including visual field (Campus Goldmann), fundus autofluorescence (FAF), optical coherence tomography (OCT) and electrophysiology. Phenotypes were classified into four categories: CSNB (N = 3, 20%) sector RP (N = 3, 20%), pericentral RP (N = 1, 6.7%) and classic RP (N = 8, 53.3% (8/15)). The phenotypes were not associated with family, sex or age (Kruskal–Wallis, p >, 0.05), however, cystoid macular edema (CME) was observed only in one family. Among the subjects reporting nyctalopia, 69% (22/32) were male. The clinical characteristics of the largest p.G90D cohort so far showed a large frequency of progressive retinal degeneration with 53.3% developing RP, contrary to the previous report.
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- 2021
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128. Razvoj sustava za praćenje uvjeta u zgradi nakon energetske obnove
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Veliki, Tomislav, Stašić, Tomislav, Grozdek, Marino, Vuletić, Damir, and Fakin, Iva
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energetska obnova zgrada ,ESCO model ,kvaliteta zraka - Abstract
Sveučilište Sjever jedina je visokoškolska institucija u RH koja se uključila u Program energetske obnove zgrada javnog sektora u razdoblju 2014. – 2020. Sljedeći korak nakon cjelovite obnove jest istraživanje parametara ugode u prostoru te je u radu opisan način na koji će se uz potrošnju energije pratiti temperatura, relativna vlažnost, razina ugljikova dioksida prisutnog u prostoru, te hlapljivi organski spojevi (VOC) u prostoru. Na temelju toga analizirat će se ostvarenje zajamčenih ušteda i procijeniti potencijal za dodatne uštede.
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- 2021
129. Razvoj sustava za praćenje uvjeta u zgradi nakon energetske obnove – 'Smart University'
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Veliki, Tomislav, Stašić, Tomislav, Grozdek, Marino, Vuletić, Damir, Fakin, Iva, and Matković, Tihomil
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energetska obnova zgrada ,ESCO model ,kvaliteta zraka - Abstract
Sveučilište Sjever je jedina visokoškolska institucija u RH koje se uključila u Program energetske obnove zgrada javnog sektora u razdoblju 2014-2020. Nakon provedene cjelovite obnove, započeto je istraživanje parametara ugode u prostoru, te su u ovom radu opisani načini na koji se uz potrošnju energije prati temperatura, RH, CO2 i VOC u prostoru. U radu su prikazani i prvi rezultati analize navedenih parametara, kao i utjecaj ponašanja korisnika na potrošnju energije. Navedeno će se promatrati u cilju unaprjeđenja postojećeg modela ugovaranja po energetskom učinku (ESCO model).
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- 2021
130. DISRUPTION OF THE OUTER SEGMENTS OF THE PHOTORECEPTORS ON OPTICAL COHERENCE TOMOGRAPHY AS A FEATURE OF VITAMIN A DEFICIENCY.
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Jevnikar, Kristina, Šuštar, Maja, Kozjek, Nada Rotovnik, Štrucl, Ana M., Markelj, Špela, Hawlina, Marko, and Fakin, Ana
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Disruption of the outer segments of the photoreceptors on optical coherence tomography may be a pathognomonic sign of vitamin A deficiency. Early detection is especially important in patients with gastrointestinal disorders who may have other underlying deficiencies. Treatment leads to resolution of visual symptoms and improves the quality of life. Purpose: To describe the optical coherence tomography features of vitamin A deficiency. Methods: Case series includes three male patients aged 50 to 66 years with vitamin A deficiency and visual symptoms ranging from 2 to 8 months. Examination included optical coherence tomography (OCT), fundus autofluorescence imaging, full-field electroretinography6 and laboratory work-up. Results: Patient 1 had inoperable pancreatic neuroendocrine tumor and presented with worsening nyctalopia. The electroretinography showed absent rod function 2 months after the onset of symptoms, followed by a decrease of the cone function eight months after the onset. Optical coherence tomography showed poorly distinguishable outer segments of the photoreceptors with the disappearance of the interdigitation zone. At that time, vitamin A deficiency along with several other deficiencies was confirmed. After the initiation of parenteral nutrition, a substantial improvement of the patient's overall well-being was noted and the OCT showed normalization of the retinal structure. Two other patients were diagnosed with vitamin A deficiency based on similar OCT features. Conclusion: Disruption of the outer segments of the photoreceptors and the disappearance of the interdigitation zone on OCT may be helpful in recognition of vitamin A deficiency. Early detection and malnutrition evaluation are especially important in patients with a history of gastrointestinal disorders who may have several other underlying deficiencies. Treatment with either enteral or parenteral nutrition not only leads to resolution of visual symptoms but vastly improves their general condition and quality of life. [ABSTRACT FROM AUTHOR]
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- 2022
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131. Metallisation of Textiles and Protection of Conductive Layers: An Overview of Application Techniques
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Ojstršek, Alenka, primary, Plohl, Olivija, additional, Gorgieva, Selestina, additional, Kurečič, Manja, additional, Jančič, Urška, additional, Hribernik, Silvo, additional, and Fakin, Darinka, additional
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- 2021
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132. Processing and functional assessment of anisotropic cellulose nanofibril/Alolt/sodium silicate: based aerogels as flame retardant thermal insulators
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Gorgieva, Selestina, primary, Jančič, Urška, additional, Hribernik, Silvo, additional, Fakin, Darinka, additional, Stana Kleinschek, Karin, additional, Medved, Sergej, additional, Fakin, Tomaž, additional, and Božič, Mojca, additional
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- 2019
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133. Changes in a knitted fabric’s surface properties due to enzyme treatments
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Jevšnik, Simona, Fakin, Darinka, Heikinheimo, Lea, and Stjepanovič, Zoran
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- 2012
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134. Barvila in razbavanje odplak: Navodila za vaje
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Selestina Gorgieva, Darinka Fakin, and Alenka Ojstršek
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Prisotnost barvil v odpadni vodi je nesprejemljiva tako iz estetskega kot tudi iz ekološkega stališča. Problematika prvega izhaja iz dejstva, da so odpadne vode, ki vsebujejo barvila, intenzivno obarvane že pri zelo nizkih koncentracijah barvil. Bolj pereča kot estetska je okoljska problematika barvil v odpadni vodi. Objektivno tveganje, ki ga predstavljajo za okolje, je odvisno od strukturnih in fizikalnih karakteristik barvil, njihove koncentracije ter časa izpostavitve v okolju. V okviru predmeta se prouči razpoložljive tehnologije čiščenja obarvanih vod, kar pa kaže na to, da ni enostavnih, učinkovitih in hkrati cenovno sprejemljivih načinov čiščenja tako kompleksnih odpadnih vod, kot so obarvane odpadne vode.
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- 2020
135. Sodobni postopki barvanja in barva: Navodila za vaje
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Alenka Ojstršek, Selestina Gorgieva, and Darinka Fakin
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Vaje »Sodobni postopki barvanja in barva« obravnavajo sistematiko in nomenklaturo barvil, fizikalno kemijo barvalnih procesov ter uporabo naprednih materialov in tehnik pri procesu barvanja. Največji del je posvečen posebnim postopkom, ki omogočajo sodoben način barvanja ob istočasnem doseganju višjega nivoja kakovosti tako v smislu ekologije, kot v doseganju več funkcionalnih lastnosti materialov in barvanju z naravnimi barvili. Postopki, ki jih posebej proučujemo so barvanje pri pogojih super kritičnega CO2, barvanje ob souporabi ultrazvoka ali predhodne plazma obdelave in barvanje s funkcionalnimi barvil. Ta vrsta barvil nam omogoča posebnosti kot so: termokromija, fotokromija, elektrokromija, solvatokromija in ostale učinke povezane z uporabo teh barvil.
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- 2020
136. Clinical and Histopathological Features of Gelsolin Amyloidosis Associated with a Novel
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Maja, Potrč, Marija, Volk, Matteo, de Rosa, Jože, Pižem, Nataša, Teran, Helena, Jaklič, Aleš, Maver, Brigita, Drnovšek-Olup, Michela, Bollati, Katarina, Vogelnik, Alojzija, Hočevar, Ana, Gornik, Vladimir, Pfeifer, Borut, Peterlin, Marko, Hawlina, and Ana, Fakin
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Adult ,Male ,Protein Folding ,Fundus Oculi ,gelsolin amyloidosis ,Glutamic Acid ,heart arrhythmia ,cutis laxa ,macromolecular substances ,Meretoja syndrome ,Article ,Corneal Diseases ,lattice corneal dystrophy ,Optic Nerve Diseases ,Humans ,Exome ,GSN ,Gelsolin ,Genetic Association Studies ,Aged ,Corneal Dystrophies, Hereditary ,Family Health ,Amyloid Neuropathies, Familial ,optical coherence tomography ,Lysine ,Optic Nerve ,Amyloidosis ,Middle Aged ,optic neuropathy ,Phenotype ,Mutation ,Female ,Amyloidosis, Familial ,Tomography, Optical Coherence - Abstract
Simple Summary Gelsolin amyloidosis is a rare autosomal dominant genetic disease, which typically affects the cornea, skin and sometimes other organ systems and is caused by mutations in a gene coding for gelsolin protein (GSN). We describe a novel mutation of GSN gene, p.Glu580Lys, associated with gelsolin amyloidosis in six members of a two-generation family, who exhibited lattice corneal dystrophy, loose facial skin and irregular heart rhythm. In one patient we reported optic nerve impairment, which is possibly a novel feature associated with gelsolin amyloidosis. Abstract Gelsolin amyloidosis typically presents with corneal lattice dystrophy and is most frequently associated with pathogenic GSN variant p.Asp214Asn. Here we report clinical and histopathological features of gelsolin amyloidosis associated with a novel GSN variant p.Glu580Lys. We studied DNA samples of seven members of a two-generation family. Exome sequencing was performed in the proband, and targeted Sanger sequencing in the others. The heterozygous GSN variant p.Glu580Lys was identified in six patients. The patients exhibited corneal dystrophy (5/6), loose skin (5/6) and/or heart arrhythmia (3/6) and one presented with bilateral optic neuropathy. The impact of the mutation on the protein structure was evaluated in silico. The substitution is located in the fifth domain of gelsolin protein, homologous to the second domain harboring the most common pathogenic variant p.Asp214Asn. Structural investigation revealed that the mutation might affect protein folding. Histopathological analysis showed amyloid deposits in the skin. The p.Glu580Lys is associated with corneal dystrophy, strengthening the association of the fifth domain of gelsolin protein with the typical amyloidosis phenotype. Furthermore, optic neuropathy may be related to the disease and is essential to identify before discussing corneal transplantation.
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- 2020
137. Visual fields in giant cell arteritis (Horton’s disease)
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Fakin, Ana, Kerin, Viktorija, and Hawlina, Marko
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- 2011
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138. Modification of Polyamide Knitted Fabric using Different Zeolites
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Karin Stana Kleinschek, Darinka Fakin, Alenka Ojstršek, and Tomaž Fakin
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Materials science ,Morphology (linguistics) ,Polymers and Plastics ,Scanning electron microscope ,Infrared spectroscopy ,wettability ,zeolites ,General Business, Management and Accounting ,Industrial and Manufacturing Engineering ,polyamide fibres ,lcsh:TP890-933 ,Polyamide ,exhaustion ,Surface modification ,lcsh:Textile bleaching, dyeing, printing, etc ,Wetting ,Business and International Management ,Composite material ,Fourier transform infrared spectroscopy ,Zeolite ,surface modification - Abstract
The aim of this research was to apply three different types of zeolites and the combination thereof in the form of a very fine powder, together with different chemicals and additives on polyamide knitted fabric according to an industrially acceptable exhaustion procedure in order to study changes in the morphology, optical properties and wettability of surfaces. Zeolites were analysed using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and gas physiosorption. Additionally, the morphology of zeolite-coated surfaces was examined closely using SEM, while changes in molecular-chemical level were examined by means of IR spectroscopy. Optical properties were studied using CIE colour measurement and diff use reflectance profi le determination, while the hydrophilic/hydrophobic character was examined using goniometry. The obtained results show the suitability of the employed exhaustion procedure, depending on the type of zeolite and the composition of the treatment bath. The results also provided evidence of the enhanced wettability of PA fabrics using 4A and 13X zeolites in combination with selected additives.
- Published
- 2017
139. Long-Term Outcomes in Breast Augmentation in Trans-Women - A 20-Year Experience
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Richard M. Fakin, Geraldine Weinforth, Pietro Giovanoli, Kai O Kaye, Lukas Lunger, Simon Zimmermann, University of Zurich, and Fakin, Richard M
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Adult ,Male ,Reoperation ,medicine.medical_specialty ,Breast Implants ,610 Medicine & health ,030230 surgery ,Preoperative care ,Transgender Persons ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Long term outcomes ,Medicine ,Inframammary fold ,Humans ,10266 Clinic for Reconstructive Surgery ,Breast augmentation ,Breast Implantation ,Retrospective Studies ,Breast tissue ,business.industry ,General Medicine ,Middle Aged ,Surgery ,2746 Surgery ,Augmentation Mammoplasty ,Hormonal therapy ,Female ,Implant ,business ,Follow-Up Studies - Abstract
Background Breast augmentation in trans-women requires special consideration and thorough preoperative planning. Objectives This study aimed to present our long-term outcome and experience gained over the past 21 years. Methods Trans-women who have undergone breast augmentation since 1995 were reviewed for primary surgery, type of incision, implant site, implant size and shape, and revisions. Results A total of 138 patients with a follow-up of 4.6 years (range, 2.0-13.3) were included. In 112 patients (82.4%), the inframammary incision was chosen. Subpectoral implantation was performed in 70 patients (51.5%). Round breast implants (91 patients, 66.9%, P = 0.010) with low projection (103 patients, 75.7%, P < 0.001) were favored. The implant size used during 2011-2016 of 363.3 ± 97.3 cc was significantly increased as compared to the implant size reported during 1995-1999 (mean difference, +142.1 cc, P < 0.001) and 2000-2004 (mean difference, +113.5 cc, P < 0.001). Simultaneously, revision rates dropped significantly over time (52.9% during 1995-1999 as compared to 6.9% during 2011-2016, P < 0.001). Overall, request for larger implants was the most common indication for revision (13 patients, 9.4%) after 6 months (range, 4.0-18.7). At revision, implant volume was 355.4 ± 132.8 cc, showing significant increase of 107.0 ± 48.1 cc as compared to primary implants (+30.0%, P = 0.048). Conclusions Request for larger breast implants was the most common reason for revision. Depending on the various degrees of breast tissue growth due to hormonal therapy, whenever applicable, we recommend round, low projection implants with a mean size of 360 cc in the prepectoral pocket. Level of Evidence: 4
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- 2019
140. Full-field electroretinography, visual acuity and visual fields in Usher syndrome: a multicentre European study
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Christoph Kernstock, Ditta Zobor, Anne Kurtenbach, Francesca Simonelli, Susanne Kohl, Stephanie Hipp, José-Alain Sahel, Marko Hawlina, Isabelle Audo, Saddek Mohand-Said, Crystel Bonnet, Christine Petit, Eberhart Zrenner, Katarina Stingl, Gesa Hahn, Francesco Testa, Ana Fakin, Institute for Ophthalmic Research [Tübingen, Germany] (Centre for Ophthalmology), University of Tübingen, University of Tuebingen, Génétique et Physiologie de l'Audition, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO), Institut de la Vision, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University Medical Centre Ljubljana [Ljubljana, Slovenia] (UMCL), University of the Study of Campania Luigi Vanvitelli, Collège de France - Chaire Génétique et physiologie cellulaire, Collège de France (CdF (institution)), Fondation Ophtalmologique Adolphe de Rothschild [Paris], University of Pittsburgh School of Medicine, Pennsylvania Commonwealth System of Higher Education (PCSHE), Tuebingen University [Germany], This work was supported by the European Union Seventh Framework Programme under the Grant Agreement HEALTH-F2-2010-242013 (TREATRUSH) and the Tistou and Charlotte Kerstan Foundation., European Project: 242013,EC:FP7:HEALTH,FP7-HEALTH-2009-single-stage,TREATRUSH(2010), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Chaire Génétique et physiologie cellulaire, Stingl, Katarina, Kurtenbach, Anne, Hahn, Gesa, Kernstock, Christoph, Hipp, Stephanie, Zobor, Ditta, Kohl, Susanne, Bonnet, Crystel, Mohand-Said, Saddek, Audo, Isabelle, Fakin, Ana, Hawlina, Marko, Testa, Francesco, Simonelli, Francesca, Petit, Christine, Sahel, Jose-Alain, and Zrenner, Eberhart
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Male ,Visual acuity ,genetic structures ,Usher syndrome ,[SDV]Life Sciences [q-bio] ,Visual Acuity ,MESH: Electroretinography ,MESH: Visual Acuity ,0302 clinical medicine ,Functional diagnostics ,MESH: Middle Aged ,medicine.diagnostic_test ,MESH: Visual Field Tests ,MESH: Retina ,MESH: European Continental Ancestry Group ,Middle Aged ,Visual field ,Sensory Systems ,Phenotype ,MESH: Young Adult ,ERG ,Female ,medicine.symptom ,Erg ,Usher Syndromes ,Retinitis Pigmentosa ,Photopic vision ,Adult ,medicine.medical_specialty ,Context (language use) ,MESH: Phenotype ,Retina ,White People ,03 medical and health sciences ,Young Adult ,Physiology (medical) ,Ophthalmology ,Retinitis pigmentosa ,medicine ,otorhinolaryngologic diseases ,Electroretinography ,Humans ,MESH: Usher Syndromes ,MESH: Humans ,business.industry ,MESH: Adult ,Functional diagnostic ,medicine.disease ,eye diseases ,MESH: Male ,030221 ophthalmology & optometry ,MESH: Retinitis Pigmentosa ,Visual Field Tests ,MESH: Visual Fields ,Visual Fields ,business ,MESH: Female ,030217 neurology & neurosurgery - Abstract
Purpose: Usher syndrome (USH) is a multisensory deficiency involving vision, hearing and the vestibular system. The purpose of this study is to report on the functional data (i.e. electroretinography, visual fields, visual acuity) of patients with retinitis pigmentosa (RP) due to Usher syndrome that were collected in a multicentre European study (TREATRUSH). Methods: A total of 268 genetically confirmed USH patients underwent electrophysiological examinations in the context of multimodal ophthalmological examination in the study (75 USH1, 189 USH2 and four USH3). Full-field electroretinography (ERG) was performed according to ISCEV standards, visual field determination was carried out with either the Octopus or Goldmann perimeters and visual acuity was examined with either ETDRS or Snellen charts. The data were compared between USH subtypes (USH1/USH2/USH3) and correlated with age. Results: Visual acuity decreases significantly with age for both USH1 and USH2 (p < 0.001), without a difference between the two cohorts. When corrected for age, the preserved kinetic visual field was significantly larger in USH2 than in USH1 (p = 0.04). Furthermore, the preserved kinetic visual field area showed a significant decrease with age (based on an exponential fit) in both USH1 and USH2 (p < 0.001). In USH1 patients, however, the visual field was already vastly reduced at an early age. The ERG results were abnormal in all patients. Detectable data for scotopic ERG were obtained from nine patients, and data of photopic ERG were obtained from 24 patients, without a difference between USH1 and USH2 subtypes. Conclusions: There are differences in the phenotypes of RP in USH subtypes, most visible in the progression of visual fields between USH1 and USH2. The perimetric reduction occurs earlier in USH1 than in USH2. In both subtypes, visual acuity decreases significantly with age and the ERG is not detectable already at early ages. Purpose: Usher syndrome (USH) is a multisensory deficiency involving vision, hearing and the vestibular system. The purpose of this study is to report on the functional data (i.e. electroretinography, visual fields, visual acuity) of patients with retinitis pigmentosa (RP) due to Usher syndrome that were collected in a multicentre European study (TREATRUSH). Methods: A total of 268 genetically confirmed USH patients underwent electrophysiological examinations in the context of multimodal ophthalmological examination in the study (75 USH1, 189 USH2 and four USH3). Full-field electroretinography (ERG) was performed according to ISCEV standards, visual field determination was carried out with either the Octopus or Goldmann perimeters and visual acuity was examined with either ETDRS or Snellen charts. The data were compared between USH subtypes (USH1/USH2/USH3) and correlated with age. Results: Visual acuity decreases significantly with age for both USH1 and USH2 (p < 0.001), without a difference between the two cohorts. When corrected for age, the preserved kinetic visual field was significantly larger in USH2 than in USH1 (p = 0.04). Furthermore, the preserved kinetic visual field area showed a significant decrease with age (based on an exponential fit) in both USH1 and USH2 (p < 0.001). In USH1 patients, however, the visual field was already vastly reduced at an early age. The ERG results were abnormal in all patients. Detectable data for scotopic ERG were obtained from nine patients, and data of photopic ERG were obtained from 24 patients, without a difference between USH1 and USH2 subtypes. Conclusions: There are differences in the phenotypes of RP in USH subtypes, most visible in the progression of visual fields between USH1 and USH2. The perimetric reduction occurs earlier in USH1 than in USH2. In both subtypes, visual acuity decreases significantly with age and the ERG is not detectable already at early ages.
- Published
- 2019
141. Analysis of Reactive Dyestuffs and Their Hydrolysis by Capillary Electrophoresis
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Ojstršek, Alenka, Doliska, Ales, and Fakin, Darinka
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- 2008
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142. Cone Dystrophy Associated with a Novel Variant in the Terminal Codon of the RPGR-ORF15
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Hadalin, Vlasta, primary, Šuštar, Maja, additional, Volk, Marija, additional, Maver, Aleš, additional, Sajovic, Jana, additional, Jarc-Vidmar, Martina, additional, Peterlin, Borut, additional, Hawlina, Marko, additional, and Fakin, Ana, additional
- Published
- 2021
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143. Stationary and Progressive Phenotypes Caused by the p.G90D Mutation in Rhodopsin Gene
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Kobal, Nina, primary, Krašovec, Tjaša, additional, Šuštar, Maja, additional, Volk, Marija, additional, Peterlin, Borut, additional, Hawlina, Marko, additional, and Fakin, Ana, additional
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- 2021
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144. Clinical and Histopathological Features of Gelsolin Amyloidosis Associated with a Novel GSN Variant p.Glu580Lys
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Potrč, Maja, primary, Volk, Marija, additional, de Rosa, Matteo, additional, Pižem, Jože, additional, Teran, Nataša, additional, Jaklič, Helena, additional, Maver, Aleš, additional, Drnovšek-Olup, Brigita, additional, Bollati, Michela, additional, Vogelnik, Katarina, additional, Hočevar, Alojzija, additional, Gornik, Ana, additional, Pfeifer, Vladimir, additional, Peterlin, Borut, additional, Hawlina, Marko, additional, and Fakin, Ana, additional
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- 2021
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145. Alkaline membrane fuel cells: anion exchange membranes and fuels
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Hren, Maša, primary, Božič, Mojca, additional, Fakin, Darinka, additional, Kleinschek, Karin Stana, additional, and Gorgieva, Selestina, additional
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- 2021
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146. Elective, Non-urgent Procedures and Aesthetic Surgery in the Wake of SARS-COVID-19: Considerations Regarding Safety, Feasibility and Impact on Clinical Management
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Dirk F. Richter, J. Benito-Ruiz, R. Escudero, Kai O Kaye, Gabriela Casabona, J. Montes, Felix J. Paprottka, T. Stasch, L. Moke, R. Fakin, University of Zurich, and Kaye, K
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Male ,COVID-19 PCR test ,COVID-19 prophylaxis ,030230 surgery ,Clinical care protocol ,030207 dermatology & venereal diseases ,COVID-19 Testing ,0302 clinical medicine ,Patient selection ,Informed consent ,Health care ,Medicine ,Longitudinal Studies ,Disease management (health) ,10266 Clinic for Reconstructive Surgery ,Risk management ,Aged, 80 and over ,Procedure flow ,Age Factors ,Disease Management ,Middle Aged ,2746 Surgery ,Patient safety ,Elective Surgical Procedures ,Original Article ,Female ,Coronavirus Infections ,Adult ,medicine.medical_specialty ,Contamination management ,SARS-Cov-2 ,Pneumonia, Viral ,Context (language use) ,610 Medicine & health ,03 medical and health sciences ,Sex Factors ,Preoperative Care ,Humans ,Surgery, Plastic ,Elective surgery ,Pandemics ,Personal Protective Equipment ,Occupational Health ,Aged ,Postoperative Care ,Infection Control ,Clinical Laboratory Techniques ,business.industry ,COVID-19 ,Evidence-based medicine ,IGM/IGG antibody rapid testing ,Surgery ,Communicable Disease Control ,Feasibility Studies ,business ,Aesthetic surgery - Abstract
Background The worldwide spread of a novel coronavirus disease (COVID-19) has led to a near total stop of non-urgent, elective surgeries across all specialties in most affected countries. In the field of aesthetic surgery, the self-imposed moratorium for all aesthetic surgery procedures recommended by most international scientific societies has been adopted by many surgeons worldwide and resulted in a huge socioeconomic impact for most private practices and clinics. An important question still unanswered in most countries is when and how should elective/aesthetic procedures be scheduled again and what kind of organizational changes are necessary to protect patients and healthcare workers when clinics and practices reopen. Defining manageable, evidence-based protocols for testing, surgical/procedural risk mitigation and clinical flow management/contamination management will be paramount for the safety of non-urgent surgical procedures. Methods We conducted a MEDLINE/PubMed research for all available publications on COVID-19 and surgery and COVID-19 and anesthesia. Articles and referenced literature describing possible procedural impact factors leading to exacerbation of the clinical evolution of COVID-19-positive patients were identified to perform risk stratification for elective surgery. Based on these impact factors, considerations for patient selection, choice of procedural complexity, duration of procedure, type of anesthesia, etc., are discussed in this article and translated into algorithms for surgical/anesthesia risk management and clinical management. Current recommendations and published protocols on contamination control, avoidance of cross-contamination and procedural patient flow are reviewed. A COVID-19 testing guideline protocol for patients planning to undergo elective aesthetic surgery is presented and recommendations are made regarding adaptation of current patient information/informed consent forms and patient health questionnaires. Conclusion The COVID-19 crisis has led to unprecedented challenges in the acute management of the crisis, and the wave only recently seems to flatten out in some countries. The adaptation of surgical and procedural steps for a risk-minimizing management of potential COVID-19-positive patients seeking to undergo elective aesthetic procedures in the wake of that wave will present the next big challenge for the aesthetic surgery community. We propose a clinical algorithm to enhance patient safety in elective surgery in the context of COVID-19 and to minimize cross-contamination between healthcare workers and patients. New evidence-based guidelines regarding surgical risk stratification, testing, and clinical flow management/contamination management are proposed. We believe that only the continuous development and broad implementation of guidelines like the ones proposed in this paper will allow an early reintegration of all aesthetic procedures into the scope of surgical care currently performed and to prepare the elective surgical specialties better for a possible second wave of the pandemic. Level of Evidence V This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
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- 2020
147. Mediji in smrt
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Fakin, Martina and Kotnik, Vlado
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spectacle ,smrt ,West ,zaključna dela ,udc:316.774:393 ,media ,spektakel ,tabloidi ,poročanje o smrti ,body ,tabuizacija ,mediji ,telo ,death ,tabooization ,Zahod ,tabloids - Published
- 2020
148. Community-Sourced Cultural Heritage Valorization Model
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Jasna Fakin Bajec
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Cultural heritage ,Instructive case ,Process (engineering) ,Interpretation (philosophy) ,Environmental ethics ,Local population ,Sociology ,Local community ,Active participation - Abstract
Cultural practices of exploring, revitalizing, interpreting and developing cultural heritage have become an important driving force in alleviating the social, cultural, environmental and economic challenges of today's world and in initiating sustainable progress in local communities. The interpretation of cultural uniqueness is not only in the hands of professionals from heritage institutions or research centres, but also in the hands of local residents, who are recognized as heritage bearers, cultural activists, practitioners and transmitters of heritage to the younger generations. The main objective of the model is to describe steps to achieve community involvement in cultural heritage projects and guidelines for carrying out the valorisation process with the active participation of the local population. The model outlines interesting and instructive case studies of how different interactive and "soft" methods can be used to build communities by following both top-down and bottom-up approaches. In addition, presented practices also show how to convince the younger generation that local heritage offers many opportunities to put bold ideas into practice.
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- 2020
149. The X-linked retinopathies: Physiological insights, pathogenic mechanisms, phenotypic features and novel therapies
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Moin D. Mohamed, Michel Michaelides, Alan C Bird, Anthony T. Moore, Andrew R. Webster, Nikolas Pontikos, Samantha R. De Silva, Gavin Arno, Omar A. Mahroo, Anthony G. Robson, and Ana Fakin
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0301 basic medicine ,Ocular albinism ,Male ,Calcium Channels, L-Type ,Retinoschisis ,Biology ,Choroideremia ,03 medical and health sciences ,0302 clinical medicine ,Cone dystrophy ,Genes, X-Linked ,Night Blindness ,Retinitis pigmentosa ,medicine ,Humans ,Alport syndrome ,Eye Proteins ,Genetics ,Retinal Degeneration ,Genetic Diseases, X-Linked ,medicine.disease ,Sensory Systems ,Ophthalmology ,030104 developmental biology ,Phenotype ,Mutation ,030221 ophthalmology & optometry ,Familial exudative vitreoretinopathy ,Female ,Norrie disease - Abstract
X-linked retinopathies represent a significant proportion of monogenic retinal disease. They include progressive and stationary conditions, with and without syndromic features. Many are X-linked recessive, but several exhibit a phenotype in female carriers, which can help establish diagnosis and yield insights into disease mechanisms. The presence of affected carriers can misleadingly suggest autosomal dominant inheritance. Some disorders (such as RPGR-associated retinopathy) show diverse phenotypes from variants in the same gene and also highlight limitations of current genetic sequencing methods. X-linked disease frequently arises from loss of function, implying potential for benefit from gene replacement strategies. We review X-inactivation and X-linked inheritance, and explore burden of disease attributable to X-linked genes in our clinically and genetically characterised retinal disease cohort, finding correlation between gene transcript length and numbers of families. We list relevant genes and discuss key clinical features, disease mechanisms, carrier phenotypes and novel experimental therapies. We consider in detail the following: RPGR (associated with retinitis pigmentosa, cone and cone-rod dystrophy), RP2 (retinitis pigmentosa), CHM (choroideremia), RS1 (X-linked retinoschisis), NYX (complete congenital stationary night blindness (CSNB)), CACNA1F (incomplete CSNB), OPN1LW/OPN1MW (blue cone monochromacy, Bornholm eye disease, cone dystrophy), GPR143 (ocular albinism), COL4A5 (Alport syndrome), and NDP (Norrie disease and X-linked familial exudative vitreoretinopathy (FEVR)). We use a recently published transcriptome analysis to explore expression by cell-type and discuss insights from electrophysiology. In the final section, we present an algorithm for genes to consider in diagnosing males with non-syndromic X-linked retinopathy, summarise current experimental therapeutic approaches, and consider questions for future research.
- Published
- 2020
150. Augmentation Mammoplasty in Trans Women
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Riccardo Lorenzini and Richard M. Fakin
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Gender dysphoria ,Trans woman ,medicine.medical_specialty ,business.industry ,Mammoplasty ,medicine.disease ,film.subject ,Surgery ,Gender reassignment surgery ,film ,Augmentation Mammoplasty ,Transgender ,medicine ,Vaginoplasty ,business ,Breast augmentation - Abstract
Breast augmentation surgery is one of the crucial procedures in male-to-female gender reassignment surgery. It is performed by implantation of silicone prostheses and/or lipofilling and can, if requested, be well integrated within the vaginoplasty surgical session, hence avoiding further anesthesia. This procedure, however, remains challenging as there are fundamental anatomical landmarks and differences between trans woman and cis woman chest. This is particularly relevant in assessing the thoracic proportions, breast and nipple positioning, their forms and sizes, as well as the synchrony with the rest of the body. Furthermore, the treatment should be personalized, addressing the patient’s individual desires and significantly contributing to the healing process of gender dysphoria.
- Published
- 2020
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