101. A new rat colon cancer cell line metastasizes spontaneously: biologic characteristics and chemotherapeutic response
- Author
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Inoue Y, Katsuyoshi Hatakeyama, Y Kashima, and Kikuo Aizawa
- Subjects
Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,Lung Neoplasms ,Spontaneous metastasis ,F344 rats ,Adenocarcinoma ,Article ,Metastasis ,In vivo ,medicine ,Tumor Cells, Cultured ,Animals ,Experimental colonic carcinoma ,Lung ,business.industry ,Mitomycin C ,Graft Survival ,Liver Neoplasms ,medicine.disease ,In vitro ,digestive system diseases ,Rats, Inbred F344 ,Rats ,Cultured cell line ,F344 rat ,medicine.anatomical_structure ,Oncology ,Cell culture ,Subserosa ,Colonic Neoplasms ,business ,Neoplasm Transplantation - Abstract
A new cell line (RCN-9) was established in culture from a transplantable rat colon adenocarcinoma, which was induced in the colon of a male Fischer F344 rat by subcutaneous administration of 1,2-dimethylhydrazine. When RCN-9 cells were injected subcutaneously or into the cecal subserosa of syngeneic rats, carcinomas with progressive growth were obtained and the development of lung (63.6%) and liver (40.0%) metastases, respectively, ensued. Antitumor effects of 5-fluorouracil (5-FU), adriamycin (ADM) and mitomycin C (MMC) against RCN-9 were examined in vivo and in vitro. 5-FU and ADM had antitumor effects both in vivo and in vitro; MMC had antitumor effects in vitro. These results show that the RCN-9 cell line can be used both as a model to study mechanisms of metastasis from colon carcinoma and as a model in chemotherapeutic studies of metastatic disease from colon carcinoma.
- Published
- 1991