The Upper Rhine Graben (URG) forms the central section of the European Cenozoic Rift System and is a mature hydrocarbon province. During previous exploration periods, about 50 oil fields and several gas fields were discovered accompanied by a huge number of exploration and production wells and numerous seismic profiles. Surprisingly, in 2003 a geothermal well found oil in the Buntsandstein reservoir and discovered unexpectedly the Römerberg oil field, which promptly doubled the total recoverable reserves (produced oil plus remaining reserves) in the German part of the URG. This discovery opened-up a new exploration play and implicated a review and revaluation of the petroleum system of the URG. Four distinct oil families occur in the URG. Three of those are of economic interest. The oldest oils are derived from the Liassic Black Shales (oil family C). These are the major source rocks for oil fields in the Alsace and southern URG as well as for the large oil fields Pechelbronn, Landau and Römerberg. Furthermore, several Tertiary source rocks exist. At many places high wax oils occur, which originated from early Tertiary (Eocene) coaly shales and sapropelic coals (oil family B). Oils from the Rupelian Fish Shale (Hochberg-Subfm.) occur especially in the northern and central URG (oil family D). In addition, highly viscous, sulphur-rich and early mature oils generated from the Corbicula- and Hydrobienschichten exist (Oil family A). These oils occur especially in the Micoene to Quaternary subsidence center, the Heidelberg-Mannheim-Graben. The oil family A oils are economically not relevant, but are of interest in order to derive kitchen areas and directions of migration.In this regard, the dissertation is subdivided into three studies. The first one focuses on a source rock characterisation of the Rupelian Fish Shale, which is the major source rock of the large oil fields Stockstadt, Eich-Königsgarten, and further smaller oil fields in the northern URG, where the Liassic source rocks are eroded. Based on petrographic and geochemical analyses the Fish Shale was characterised as good to very good source rock containing typical type II kerogens, 4-5.5 % TOC and Hydrogen Index (HI) values of 450-550 mg HC/g TOC (at an immature state). Special biomarkers such as characteristic sterane distributions, age-related markers such as oleanane, or a highly branched isoprenoid C25 with origin from marine diatoms enable reliable oil-source rock correlations. The Fish Shale is distributed in the entire graben in uniform facies. Thus, the thickness and thermal maturity are the factors controlling the amount of generated hydrocarbons. The latter is the limiting factor for missing oils derived from the Fish Shale in the Alsace and southern URG (south of Karlsruhe). This was shown by a vitrinite reflectance map, which distinctly identifies kitchen areas of the Fish Shale. Existing reservoired oils derived from the Fish Shale were correlated to these kitchen areas and migration pathways and directions were identified.The second part analyses the Liassic source rocks with focus on their thermal maturity. An investigation beyond the borders of the URG showed that the Posidonia Shale (Lias ε) reached a maturity of about 0.5-0.6 % VRr within the graben area and its surroundings before the formation of the URG in the Eocene. Liassic outcrops situated at the graben margin, in the Alsace, in the Swabian Alb, in the foreland of the Alps, and samples from shallow wells (ca. < 1000-1500 m) within the graben show a quite uniform maturity, which results from a relatively coherent burial history and temperature increase before the formation of the URG. In addition, this maturity indicates that the Liassic source rocks did not expel a significant quantity of oil before the formation of the graben. A map of the thermal maturity of the Posidonia Shale reveals a mid-oil window mature source rock in major parts of the central URG, which also reached the wet gas window in the area of Karlsruhe.The final third part gives an overview on source rocks and the petroleum system of the URG. Especially the Posidonia Shale can be characterised as excellent source rock and generated most oil in the URG. High mean TOC values of 8 % and HI values of > 550 mg HC/g TOC characterise the Posidonia Shale at an immature stage. Moreover, the bituminous Lias α marls and shales have important additional source rock potential and contributed to the oils of family C.Furthermore, new insights on source rocks of the different oil families and several new oil-source rock correlations are presented and the distribution of oil families in regard to migration pathways is discussed. The high wax oils (Family B) are heterogeneously distributed, but occur especially in the northern and central URG, in the latter predominantly in the western part. These oils are stemming from early Tertiary, locally occurring, coaly shales and sapropelic coals. The hydrocarbon generation potential of these source rocks is rather difficult to evaluate because of significant variations in thickness and source rock characteristics. Rock-Eval pyrolysis and bulk kinetic measurements of the sapropelic coals indicate a partly extraordinary high oil generation potential. These source rocks contributed also to the large Landau oil field. Interestingly, the large oil fields Pechelbronn, Landau and Römerberg, which are all predominantly charged by the Liassic source rocks, occur in areas where the Liassic source rocks are already eroded or immature, in case of Pechelbronn. Consequently, oils of the Liassic source rocks migrated mainly laterally in northwestern directions and successively into older stratigraphic units. The Opalinuston-Fm., Liassic claystones and basal Tertiary marls are acting in this context as important seals. In this progress, faults provide - in particular for the Pechelbronn field with numerous small, segmented fault blocks in the fetch area - entry points especially into younger (Tertiary) but also older (Triassic) reservoirs. However, as a consequence of the structural configuration during the early formation of the URG, faults are not indispensable for the migration of Liassic oil into older stratigraphic units. All in all, a comprehensive overview on the distribution of oil families in the URG with respect to their vertical and lateral migration pathways is given in this thesis.