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482 results on '"Engelmann, K."'

103. Expression of genes coding growth factors and growth factor receptors in differentiated and dedifferentiated human corneal endothelial cells

Author

H. A. Weich, Engelmann K, Bednarz J, and Rodokanaki-von Schrenck A

Subjects
Corneal Stroma, Gene Expression, Biology, Filaggrin Proteins, Vascular endothelial growth inhibitor, Fibroblast growth factor, Epithelium, chemistry.chemical_compound, Growth factor receptor, Animals, Humans, Growth factor receptor inhibitor, Receptors, Growth Factor, Growth Substances, Cells, Cultured, Endothelium, Corneal, Cell Differentiation, DNA, Fibroblasts, Blotting, Northern, Cell biology, Vascular endothelial growth factor B, Vascular endothelial growth factor, Ophthalmology, Vascular endothelial growth factor A, Vascular endothelial growth factor C, chemistry, RNA, Cattle, sense organs, DNA Probes
Abstract

Growth and differentiation of human corneal endothelial cells are shown to be influenced by growth factors and by the extracellular matrix. Under standard culture conditions, these cells tend to dedifferentiate to a fibroblast-like phenotype. The aim of this study was to examine the expression of genes encoding growth factors and growth factor receptors in differentiated and dedifferentiated human corneal endothelial cells and in keratocytes (stromal fibroblasts) by means of Northern blot analysis. Expression of genes coding basic fibroblast growth factor (FGF), FGF receptor-1 (flg-type), epidermal growth factor receptor, and transforming growth factor-beta 1 in keratocytes and human corneal endothelial cells could be confirmed. In contrast, significant expression of the gene encoding acidic FGF was not detected. However, expression of the genes encoding vascular endothelial growth factor (VEGF) and the high-affinity tyrosine kinase receptor for VEGF (flt-1) in human corneal endothelial cells could be demonstrated for the first time. Moreover, exogenous basic FGF seemed to have a positive influence on the VEGF gene expression level. The flt-1 gene was expressed in dedifferentiated, but not in differentiated human corneal endothelial cells. The results indicate a possible role of VEGF in the differentiation of human corneal endothelial cells.

Published
1995

106. Untersuchungen zur Nachstarprävention in vitro mittels des zyklischen RGD-Peptids cRGD D FV

Author

Pham, D. T., Engelmann, K., Rieck, P., Kojetinsky, Corina, Pham, D. T., Engelmann, K., Rieck, P., and Kojetinsky, Corina

Abstract

Hintergrund: RGD-Peptide hemmen kompetitiv die Adhäsionsmoleküle von Linsenepithelzellen (LEC). Ziel unserer Untersuchungen war es herauszufinden, ob diese Peptide in der Lage sind, auch nach Kurzzeitinkubation eine suffiziente Inhibition der Adhäsion bzw. eine Ablösung adhärenter Zellen und damit eine ausreichende Prävention des Nachstars zu bewirken. Außerdem wurde überprüft, ob das von uns verwendete RGD-Peptid eine Toxizität für die Hornhaut aufweist. Material und Methoden: Kulturen boviner und humaner LEC, boviner Hornhautendothelzellen, humane und bovine Linsenkapselexzidate und humane explantierte Hornhäute wurden verwendet. Wir untersuchten die Inhibition der Adhäsion und die Ablösung konfluenter LEC-Layer mittels des zyklischen RGD-Peptids cRGDDFV (Inkubationszeiten von 1 Stunde bzw. 5-7 Tagen und Konzentrationen von 10-4 M, 10-3 M und 2x10-3 M wurden angewandt). Ergebnisse: Wir fanden nach nur einstündiger Inkubation in der Kulturschale eine Adhäsionsinhibition von 48% für bovine LEC und von 100% für humane LEC. Die Differenz zwischen Kontrollpeptid und cRGDDFV war statistisch signifikant (p, Purpose: RGD-peptides competitively inihibit adhesion molecules of the lens epithelial cells (LEC). The purpose of our study was to investigate whether this peptide could be able to inhibit adhesion sufficiently after short term incubation resp. to detach adherent cells and so to prevent posterior capsule opacification (PCO). Also there was proofed if there is any toxicity for the cornea. Methods: Cultures of bovine and human LEC, bovine cornea endothelial cells, humane and bovine fragments of the lens capsule and explanted humane corneas were used. The inhibition of adhesion and the detachment of confluent LEC-layer by the cyclic RGD-peptide cRGDDFV were studied (incubation time was 1 hour resp. 5-7 days and concentrations of 10-4, 10-3 M and 2x10-3 M were used). Results: After one hour incubation time in a culture dish inhibition of adhesion was 48% for bovine LEC resp. 100% for humane LEC. There was a statistically significant difference between the control-peptide-group and cRGDDFV (p

Published
2002

148. Tumour Pathology, Abstract 226–234, Posters

Author

Boltze, C., primary, Boltze, C., additional, Mahotka, C., additional, Hassan, M., additional, Braun, Y., additional, Engelmann, K., additional, Went, P.H., additional, Herter, P., additional, and Kristiansen, G., additional

Published
2003
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149. Rheopherese

Author

Fell, A. J., primary, Engelmann, K., additional, Richard, G., additional, Fassbender, C., additional, Wahls, W., additional, and Klingel, R., additional

Published
2002
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