118 results on '"Emery, Sanford E."'
Search Results
102. Delayed Fracture of Fibular Strut Allograft Following Three-Level Anterior Cervical Corpectomy and Fusion: A Case Report.
- Author
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McConda, David B. and Emery, Sanford E.
- Published
- 2015
103. Magnetic Resonance Imaging of Posttraumatic Spinal Ligament Injury.
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Emery, Sanford E., Pathria, Mini N., Wilber, R. Geoffrey, Masaryk, T., and Bohlman, Henry H.
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- 1989
104. The pathophysiology of cervical spondylosis and myelopathy.
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Bohlman, Henry H., Emery, Sanford E., Bohlman, H H, and Emery, S E
- Published
- 1988
105. Reactions of Sodium Borohydride in Acidic Media; VII. Reduction of Diaryl Ketones in Trifluoroacetic Acid
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GRIBBLE, Gordon W., primary, KELLY, William J., additional, and EMERY, Sanford E., additional
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- 1978
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106. ThreeLevel Anterior Cervical Discectomy and Fusion
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Emery, Sanford E., Fisher, Rush J.S., and Bohlman, Henry H.
- Abstract
A retrospective study of 16 patients who underwent the modified Robinson anterior cervical discectomy and fusion at three operative levels.
- Published
- 1997
107. ABOS & MOC: Value for Our Profession.
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EMERY, SANFORD E.
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PHYSICIAN practice patterns , *TECHNICAL specifications , *PATIENT safety , *CERTIFICATION - Abstract
The article discusses the withdrawal of some of the instituted requirements for Maintenance of Certification (MOC) by the American Board of Internal Medicine (ABIM). Topics discussed include the removal of the requirements on Practice Assessment, Patient Voice, and Patient Safety, the recertification made through the acceptance of the MOC concept, and the ways sought by the American Board of Orthopaedic Surgery (ABOS) for the improvement of MOC.
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- 2015
108. CHAPTER 30 - Interbody Strut Techniques
- Author
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Saunders, Richard L., Traynelis, Vincent C., and Emery, Sanford E.
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109. Contributors
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Abel, Mark F., Abumi, Kuniyoshi, Adams, Mark S., Alberstone, Cary D., Alexander, Joseph T., Anson, John A., Apfelbaum, Ronald I., Arnold, Paul M., Babat, L. Brett, Bailes, Julian E., Baisden, Jamie, Baldwin, Nevan G., Ball, Perry A., Barolat, Giancarlo, Batjer, H. Hunt, Bauer, Thomas W., Bean, James R., Beerle, Brion J., Bell, Gordon R., Bennett, Gregory J., Benzel, Edward C., Bergey, Darren, Bertrand, Marc L., Bilsky, Mark H., Birch, Barry D., Biscup, Robert S., Blaylock, Kevin, Boachie-Adjei, Oheneba, Boakye, Maxwell, Boden, Scott D., Bohlman, Henry, Bolesta, Michael, Bondy, Mary B., Boxell, Christopher M., Bridwell, Keith H., Brodke, Darrell S., Butler, James, Cahill, David W., Cantu, Robert C., Carl, Allen L., Carrino, John A., Caruso, John R., Chenelle, Andrew G., Cheng, Joseph S., Cho, Yong-Jun, Choudhri, Tanvir F., Conguista, Frank, Connolly, Edward S., Cooper, Paul R., Coumans, Jean-Valéry C.E., Cram, Albert E., Crockard, H. Alan, Crownover, Richard, Cunningham, Bryan W., Curry, William T., Jr, Cusick, Joseph F., Daffner, Scott D., D'Alise, Mark D., Deshmukh, Vinay, DiAngelo, Denis, Dickman, Curtis A., Ducker, Thomas B., Dull, Scott T., Dunsker, Stewart B., Ebersold, Michael J., Eckhardt, Jason, Ehni, Bruce L., Eichenbaum, Matthew, Eichholz, Kurt M., Eichler, Marc E., Elbabaa, Samer K., Emery, Sanford E., Epstein, Nancy E., Erdos, Jennifer, Errico, Thomas J., Faciszewski, Tom, Fehlings, Michael G., Ferrara, Lisa A., Fessler, Richard G., Foley, Kevin T., Galler, Robert M., German, John W., Ghanayem, Alexander J., Ghogawala, Zoher, Goel, Vijay K., Goffin, Jan, Gokaslan, Ziya L., Gollogly, Sohrab, Gonzalez-Martinez, Jorge, Greensmith, James E., Gross, Jeffrey D., Haid, Regis W., Jr, MD, Andrea L. Halliday, Hamilton, Allan J., Hanbali, Fadi, Harms, Jürgen, Harrop, James S., Hart, Blaine I., Hart, Robert A., Heary, Robert F., Henderson, Fraser C., Hitchon, Patrick W., Hollowell, James P., Holman, Paul J., Houten, John K., Isaacs, Robert E., Ito, Manabu, Jane, John A., Jr, Johnson, J. Patrick, Kager, Christopher, Kalfas, Iain H., Kaptain, George J., Khairi, Saad, Kim, Daniel H., Kim, David H., Kopitnik, Thomas A., Jr., Kowalski, Robert J., Krishnaney, Ajit A., Lancon, John A., Lanzino, Giuseppe, Larson, Sanford J., Lastra-Power, Jorge, Lebwohl, Nathan H., Lieberman, Isador H., Long, Donlin M., Luciano, Mark G., Luevano, Charles A., Madsen III, Parley M., Maiman, Dennis J., Malik, Jacek M., Malone, David G., Maroon, Joseph C., Massicotte, Eric M., Matsunaga, Shunji, Mazanec, Daniel J., McAfee, Paul C., McCormack, Bruce M., McCormick, Paul C., McCormick, William E., McGuire, Robert A., Jr., McLain, Robert F., Mekhail, Nagy, Melton, D. Mark, Miller, Carole A., Miller, Jared H., Min, Sung, Mitchell, William, Mizuno, Junichi, Modic, Michael T., Morgan, Howard W., Jr, Morlock, Robert J., Morone, Michael A., Mueller, Wade M., Mummaneni, Praveen V., Myseros, John S., Naderi, Sait, Nair, Dileep, Nakagawa, Hiroshi, Nieto, Jaime H., Nockels, Russ P., Northrup, Bruce E., Ohaegbulam, Chima, Oktenoglu, Tunc, Ordonez, Bernardo Jose, Owen, Jeffrey H., Özer, A. Fahir, Papadopoulos, Stephen M., Paramore, Christopher G., Pashman, Robert S., Peacock, Warwick J., Pelofsky, Stanley, Perin, Noel I., Pham, Christopher J., Placide, Rick J., Prpa, Branko, Przybylski, Gregory J., Ragab, Ashraf A., Rampersaud, Y. Raja, Rasmussen, Peter A., Raynor, Richard B., Rea, Gary L., Rechtine, Glenn R., Regan, John, Rengachary, Setti S., Resnick, Daniel K., Rhines, Laurence D., Rhoton, Albert J., Rodriguez, Donna J., Rodts, Gerald E., Jr, Rosner, Michael J., Sah, Alexander, Salinsky, Jared P., Santiago, Paul, Sarkarati, Mehdi, Saunders, Richard L., Sawin, Paul D., Scheid, Edward H., Schmidt, Meic H., Schneier, Michael, Sengupta, Dilip K., Shaffrey, Christopher I., Shaffrey, Mark E., Sharan, Alok D., Sharan, Ashwini D., Shields, Christopher B., Simeone, Frederick A., Singh, Kern, Singh, Ran Vijai P., Smith, Donald A., Smith, Maurice M., Sonntag, Volker K.H., Sosa, Ivan J., Speck, Micheal J., Spetzler, Robert F., Sridharan, Sudhakar T., Staudt, Loretta A., Steinmetz, Michael P., Stillerman, Charles B., Suda, Kota, Suys, Sonia, Sypert, George W., Tator, Charles H., Theodore, Nicholas, Thomas, Ajith J., Thomas, Nicholas W.M., Tibbs, Robert E., Jr., Togawa, Daisuke, Tomecek, Frank J., Toselli, Richard M., Traynelis, Vincent C., Trost, Gregory R., Truumees, Eeric, Tye, Gary W., Uddin, Abm Salah, Vaccaro, Alexander R., Vaicys, Ceslovas, Valadka, Alex, Vardiman, Arnold B., Virella, Anthony A., Vitarbo, Elizabeth, Vitaz, Todd W., Vollmer, Dennis G., Voyadzis, Jean-Marc, Ward, John D., Watson, Joseph, Webb, John K., Weinstein, Philip R., Weiser, Martin W., Welch, William C., Weller, Simcha J., Westerlund, L. Erik, White, Jonathan A., Whitfield, Melvin D., Wiggins, Gregory C., Wilberger, Jack E., Wilke, William S., Wiseman, Diana Barrett, Wolcott, W. Putnam, Woodard, Eric J., Yazback, Philip, Yoganandan, Narayan, Yonemura, Kenneth S., Yonenobu, Kazuo, Yuan, Hansen A., Zeidman, Seth M., Zide, Barry M., and Zileli, Mehmet
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110. Why Do Medical Students Choose Orthopaedics as a Career?
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Johnson, Amanda L., Sharma, Jyoti, Chinchilli, Vernon M., Emery, Sanford E., Evarts, C. McCollister, Floyd, Mark W., Kaeding, Christopher C., Lavelle, William F., Marsh, J. Lawrence, Pellegrini Jr., Vincent D., Van Heest, Ann E., and Black, Kevin P.
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ORTHOPEDICS , *MEDICAL students , *MEDICAL schools , *CLINICAL trials , *HEALTH occupations students , *DENTAL students , *EDUCATION - Abstract
Background: The primary influence on medical students' career choice is their third-year clerkship. However, orthopaedics is not a required rotation in the curriculum of most medical schools. Our goals were to identify factors that motivate students to choose an orthopaedic career and to compare these with the factors that influence students to choose nonorthopaedic disciplines. Methods: Fourth-year medical students and orthopaedic residents at the postgraduate year (PGY)-l level at eight orthopaedic training programs in the United States were surveyed to determine the reasons that they chose orthopaedics instead of other medical or surgical fields. Results: Of the 622 individuals who responded to our survey, 125 were entering orthopaedics and 497 were not. Although career choice in both groups was most heavily influenced by third and fourth-year clinical rotations and faculty contacts, orthopaedics-bound respondents were more likely than non-orthopaedics-bound respondents to be strongly influenced by experiences and people prior to medical school. Orthopaedics-bound respondents were less likely to report a faculty member as the most important person influencing career choice. Fifty-one percent (sixty-three) of 124 students who selected orthopaedics had already decided to pursue this field prior to their third-year rotation. Patient care was chosen by 71% (347) of490 non-orthopaedics-bound respondents and 75% (ninety-four) of 125 orthopaedics-bound respondents as the most important factor for pursuing a particular field. Income was not selected as the deciding factor by respondents in either group. Conclusions: Although faculty contacts and third-year clinical rotations played an important role in student selection of specialty training, they were less influential for those choosing an orthopaedic career than for those choosing other disciplines. Many students choosing orthopaedics made this decision prior to medical school. We believe that increased exposure to positive clinical role models and experiences during medical school would enhance medical students' options for choosing orthopaedic surgery as a career. Anticipated income did not play a deciding role in career selection. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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111. The Occurrence of Wrong-Site Surgery Self-Reported by Candidates for Certification by the American Board of Orthopaedic Surgery.
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James, Michellc A., Seller III, John Gray, Harrast, John I., Emery, Sanford E., and Hurwitz, Shepard
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- *
ORTHOPEDISTS , *ORTHOPEDICS , *SURGEONS , *PREOPERATIVE care , *SOCIETIES ,PREVENTION of surgical complications - Abstract
Background: The concept of "Sign Your Site" was established in 1997 to prevent wrong-site surgery in the U.S., and this was expanded to the mandated Universal Protocol in 2008. However, the true incidence of wrong-site surgery in the U.S. is not known. nor do we know whether the incidence has changed. The American Board of Orthopaedic Surgery (ABOS) requires that candidates for Board certification provide a list of their cases, including surgical complications, whether wrong-site surgery was performed. and whether they complied with the "Sign Your Site" practice. Each candidate attests to the accuracy of his or her notarized case list. The purpose of this study was to report the incidence and nature of wrong-site surgery self-reported by orthopaedic surgeon candidates for certification between 1999 and 2010 and to assess whether any change was associated with the timeline of implementation of the Universal Protocol. Methods" The ABOS database was queried for the number of examinees, cases, and wrong-site surgery cases reported; a description of each wrong-site surgery case: whether the site was signed; and the surgeon's subspecialty. Results: From 1999 through 2010. 9255 orthopaedic surgeons submitted 1,291,396 cases, and sixty-one of these surgeons reported performing seventy-six wrong-site surgical procedures. Spine surgeons were the most likely to report wrong-site surgery, most commonly singleqevet lumbar laminotomy. The rate of wrong-site surgical procedures before and after implementation of the Universal Protocol mandate was not significantly different. Eighteen of the twenty wrong-site surgical procedures performed since ABOS data collection for "Sign Your Site" began had been signed preoperatively. Conclusions" Keeping patients safe remains an essential goal worthy of enormous effort. This study suggests that additional layers of precautions may yield diminishing returns and that attention should be focused on methods to prevent wrong-level spine surgery. Improving communication among the health-care team and shared responsibility may bring us closer to eliminating wrong-site surgery. [ABSTRACT FROM AUTHOR]
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- 2012
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112. Cervical fusion for treatment of degenerative conditions: development of appropriate use criteria.
- Author
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Reitman CA, Hills JM, Standaert CJ, Bono CM, Mick CA, Furey CG, Kauffman CP, Resnick DK, Wong DA, Prather H, Harrop JS, Baisden J, Wang JC, Spivak JM, Schofferman J, Riew KD, Lorenz MA, Heggeness MH, Anderson PA, Rao RD, Baker RM, Emery SE, Watters WC 3rd, Sullivan WJ, Mitchell W, Tontz W Jr, and Ghogawala Z
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- Cervical Vertebrae diagnostic imaging, Cervical Vertebrae surgery, Humans, Treatment Outcome, Radiculopathy, Spinal Cord Diseases, Spinal Diseases diagnostic imaging, Spinal Diseases surgery, Spinal Fusion
- Abstract
Background Context: High quality evidence is difficult to generate, leaving substantial knowledge gaps in the treatment of spinal conditions. Appropriate use criteria (AUC) are a means of determining appropriate recommendations when high quality evidence is lacking., Purpose: Define appropriate use criteria (AUC) of cervical fusion for treatment of degenerative conditions of the cervical spine., Study Design/setting: Appropriate use criteria for cervical fusion were developed using the RAND/UCLA appropriateness methodology. Following development of clinical guidelines and scenario writing, a one-day workshop was held with a multidisciplinary group of 14 raters, all considered thought leaders in their respective fields, to determine final ratings for cervical fusion appropriateness for various clinical situations., Outcome Measures: Final rating for cervical fusion recommendation as either "Appropriate," "Uncertain" or "Rarely Appropriate" based on the median final rating among the raters., Methods: Inclusion criteria for scenarios included patients aged 18 to 80 with degenerative conditions of the cervical spine. Key modifiers were defined and combined to develop a matrix of clinical scenarios. The median score among the raters was used to determine the final rating for each scenario. The final rating was compared between modifier levels. Spearman's rank correlation between each modifier and the final rating was determined. A multivariable ordinal regression model was fit to determine the adjusted odds of an "Appropriate" final rating while adjusting for radiographic diagnosis, number of levels and symptom type. Three decision trees were developed using decision tree classification models and variable importance for each tree was computed., Results: Of the 263 scenarios, 47 (17.9 %) were rated as rarely appropriate, 66 (25%) as uncertain and 150 (57%) were rated as appropriate. Symptom type was the modifier most strongly correlated with the final rating (adjusted ρ2 = 0.58, p<.01). A multivariable ordinal regression adjusting for symptom type, diagnosis, and number of levels and showed high discriminative ability (C statistic = 0.90) and the adjusted odds ratio (aOR) of receiving a final rating of "Appropriate" was highest for myelopathy (aOR, 7.1) and radiculopathy (aOR, 4.8). Three decision tree models showed that symptom type and radiographic diagnosis had the highest variable importance., Conclusions: Appropriate use criteria for cervical fusion in the setting of cervical degenerative disorders were developed. Symptom type was most strongly correlated with final rating. Myelopathy or radiculopathy were most strongly associated with an "Appropriate" rating, while axial pain without stenosis was most associated with "Rarely Appropriate.", (Copyright © 2021 Elsevier Inc. All rights reserved.)
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- 2021
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113. Challenges and Solutions to Academic Orthopaedics in Current Health-Care Economics: AOA Critical Issues.
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Obremskey WT, Emery SE, and Alman BA
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- Humans, Societies, Medical, Academic Medical Centers economics, Orthopedics economics
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- 2020
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114. Complexities of spine surgery in obese patient populations: a narrative review.
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Katsevman GA, Daffner SD, Brandmeir NJ, Emery SE, France JC, and Sedney CL
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- Body Mass Index, Humans, Lumbar Vertebrae, Postoperative Complications epidemiology, Postoperative Complications etiology, Retrospective Studies, Treatment Outcome, Obesity complications, Obesity epidemiology, Spinal Fusion
- Abstract
The obese population is particularly challenging to the spine surgeon in all phases of care. A narrative literature review was performed to review difficulties in spine surgery on the obese patient population and techniques for mitigation. We specifically aimed to assess several topics with regard to this population: patient selection and preoperative care; intraoperative and surgical techniques; and postoperative care, outcomes, and complications. The literature review demonstrated that obese patients are at increased surgical risk with spine surgery due to a variety of factors at all stages of intervention. Preoperatively, obese patients have worse outcomes with physical therapy and present technical difficulties for injections. Transport to a hospital, imaging, resuscitation, and intubation are all challenged by increased body habitus. Intraoperatively, obese patients have increased operative times, blood loss, surgical site infections, and nerve palsies. Patient positioning and intraoperative imaging may be limited. Surgery itself may be technically challenging due to body habitus and minimally invasive techniques are becoming more prevalent in this population. Postoperatively, several studies demonstrate that obese patients have inferior outcomes compared with nonobese counterparts. Patient selection is a key for elective interventions, and appropriate infrastructure aids in the ultimate outcomes for both elective and nonelective surgical treatments. Overall, obese patients present several challenges to the spine surgeon, and certain precautions can be undertaken preoperatively, intraoperatively, and postoperatively to mitigate the associated risks to optimize outcomes., (Copyright © 2019 Elsevier Inc. All rights reserved.)
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- 2020
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115. The Effects of Perioperative Corticosteroids on Dysphagia Following Surgical Procedures Involving the Anterior Cervical Spine: A Prospective, Randomized, Controlled, Double-Blinded Clinical Trial.
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Cui S, Daffner SD, France JC, and Emery SE
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- Diskectomy adverse effects, Diskectomy statistics & numerical data, Double-Blind Method, Female, Humans, Male, Middle Aged, Perioperative Care methods, Prospective Studies, Spinal Fusion adverse effects, Spinal Fusion statistics & numerical data, Treatment Outcome, Adrenal Cortex Hormones administration & dosage, Cervical Vertebrae surgery, Deglutition Disorders prevention & control, Dexamethasone administration & dosage, Postoperative Complications prevention & control
- Abstract
Background: Dysphagia is one of the most common complications of surgical procedures in the anterior cervical spine, and can persist up to 2 years postoperatively. Corticosteroids are relatively safe and inexpensive for treating various inflammatory conditions. Perioperative corticosteroid administration for anterior cervical spine procedures may effectively minimize postoperative dysphagia, potentially leading to better outcomes, decreased readmission rates, and improved patient satisfaction. The purpose of this study was to determine the efficacy of perioperative corticosteroids in decreasing the severity and duration of dysphagia following single-level and multilevel anterior cervical spine procedures., Methods: Seventy-four patients undergoing elective anterior cervical surgical procedures for degenerative conditions were recruited. Patients with prior cervical procedures; with a diagnosis of fracture, malignancy, or infection; or requiring combined anterior-posterior procedures were excluded. Patients were randomized to perioperative intravenous dexamethasone or saline solution. Doses were administered before incision and at 8 and 16 hours postoperatively. Investigators and patients were blinded to the treatment throughout the study. Dysphagia outcomes were assessed with use of the Bazaz dysphagia scale and the Dysphagia Short Questionnaire (DSQ) at 1 day, 2 days, 1 week, 2 weeks, 1 month, 3 months, 6 months, and 12 months postoperatively. Statistical analysis was performed comparing means and standard deviations; significance was set at p < 0.05. Clinical outcomes were measured with use of the Quality of Life-12 and Neck Disability Index., Results: Sixty-four patients were included in the analysis. There were 49 anterior cervical discectomies and fusions, 8 corpectomies, 1 hybrid procedure (corpectomy and adjacent discectomy), and 6 single-level arthroplasties. Patients who received corticosteroids had significantly better dysphagia scores on both the Bazaz scale and DSQ at most time points up to 6 months postoperatively (p < 0.05). On subgroup analysis, patients with multilevel (≥2-level) fusion benefited significantly from corticosteroids on both scales, whereas those with single-level procedures did not. There were no short-term wound complications or infections, and length of stay and fusion rates were comparable., Conclusions: Perioperative administration of corticosteroids can reduce dysphagia symptoms following multilevel anterior cervical procedures. Benefit was noted immediately and up to 6 months postoperatively. There was no significant effect on short-term wound-healing, infection rates, length of stay, or fusion rates., Level of Evidence: Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
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- 2019
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116. Diversity in Orthopaedic Surgery: International Perspectives: AOA Critical Issues.
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Emery SE
- Subjects
- Ethnicity statistics & numerical data, Humans, Cultural Diversity, Faculty, Medical statistics & numerical data, Orthopedics statistics & numerical data, Workforce statistics & numerical data
- Abstract
Orthopaedic surgery in the United States is one of the few medical specialties that has consistently lacked diversity in its training programs and workforce for decades, despite increasing awareness of this issue. Is this the case in other English-language speaking countries? Are there inherent national differences, or does orthopaedics as a profession dictate the diversity landscape around the globe?The Carousel group includes the presidents of the major English-language-speaking orthopaedic organizations around the globe-Australia, Canada, New Zealand, South Africa, the United Kingdom, and the United States. Established in 1952, members of this group attend each other's annual scientific meetings during the year of their presidency, learning about our profession in each country and building international relationships. In this article, 13 Carousel presidents from different countries explore diversity in orthopaedics in their training programs and the workforce, with an assessment of the current state and ideas for improvement.
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- 2019
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117. Lateral mass screw fixation in the cervical spine.
- Author
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Coe JD, Vaccaro AR, Dailey AT, Sasso RC, Ludwig SC, Harrop JS, Dettori JR, Shaffrey CI, Emery SE, and Fehlings MG
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- Female, Humans, Male, Cervical Vertebrae surgery, Kyphosis etiology, Kyphosis surgery, Neurofibromatosis 1 complications, Spinal Fusion methods
- Published
- 2014
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118. Coculture of synovium-derived stem cells and nucleus pulposus cells in serum-free defined medium with supplementation of transforming growth factor-beta1: a potential application of tissue-specific stem cells in disc regeneration.
- Author
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Chen S, Emery SE, and Pei M
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- Aggrecans drug effects, Aggrecans metabolism, Animals, Biomarkers analysis, Biomarkers metabolism, Cell Communication drug effects, Cell Communication physiology, Cell Differentiation drug effects, Cell Differentiation physiology, Cell Proliferation drug effects, Cells, Cultured, Chondrogenesis drug effects, Chondrogenesis physiology, Coculture Techniques methods, Collagen Type II drug effects, Collagen Type II metabolism, Culture Media, Serum-Free pharmacology, Dose-Response Relationship, Drug, Hypoxia-Inducible Factor 1, alpha Subunit drug effects, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Intervertebral Disc cytology, Intervertebral Disc metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Regeneration physiology, SOX9 Transcription Factor drug effects, SOX9 Transcription Factor metabolism, Subcellular Fractions, Sus scrofa, Transforming Growth Factor beta1 metabolism, Up-Regulation drug effects, Up-Regulation physiology, Intervertebral Disc drug effects, Intervertebral Disc Displacement surgery, Mesenchymal Stem Cell Transplantation methods, Mesenchymal Stem Cells drug effects, Regeneration drug effects, Transforming Growth Factor beta1 pharmacology
- Abstract
Study Design: A coculture of synovium-derived stem cells (SDSCs) and nucleus pulposus cells (NPCs) in a serum-free pellet system was treated with varying doses of transforming growth factor beta (TGF-beta). Cultures of either SDSCs or NPCs alone served as controls., Objective: The aim was to assess the feasibility of using SDSCs to supplement and replenish NPC population for disc regeneration., Summary of Background Data: SDSCs have been proven to be a tissue-specific type of mesenchymal stem cell capable of chondrogenesis. NPCs are chondrocyte-like cells with a high ratio of aggrecan. However, the capacity of SDSCs to complement the NPC population is not known., Methods: SDSCs were negatively isolated from porcine knee joint synovial tissue and NPCs were isolated from porcine lumbar spines (L1-L5). SDSCs and NPCs were cocultured (50:50) in a serum-free pellet system with the supplementation of varying doses (0, 3, 10, and 30 ng/mL) of TGF-beta1 for 14 days. SDSCs or NPCs cultured alone served as controls. Chondrogenic differentiation markers were evaluated by histology, immunohistochemistry, biochemistry, and TaqMan PCR., Results: The coculture of SDSCs and NPCs in a pellet system displayed comparable differentiation properties (high levels of collagen II, aggrecan and Sox 9, a low level of collagen I, and no collagen X detectable) to NPCs alone when treated with high doses of TGF-beta1. Moreover, the coculture and NPCs alone shared a similar higher ratio of aggrecan to collagen II. Hypoxia-inducible factor 1alpha (HIF-1alpha) was also observed to be up-regulated in coculture pellets at day 7 and had decreased at day 14 with the time of pellet tissue maturation., Conclusion: SDSCs may act as a potential mesenchymal stem cell candidate for NP regeneration. Further studies are needed to evaluate the in vivo effect of SDSCs on disc regeneration.
- Published
- 2009
- Full Text
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