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Anti-HLA-DR-Antikorper sind derzeit die meistverwendeten Antikorper zur Darstellung der kornealen Langerhans-Zelle (LZ). Diese Arbeit untersucht das Verteilungsmuster von S-100-Protein und CD45 an der LZ verglichen mit HLA-DR. Acht frische Hornhaute wurden in flussigem Stickstoff fixiert und mit einer Dicke von 4–6 μm geschnitten. Anschliesend wurde eine doppelte immunhistochemische Farbung (HLA-DR/S-100 oder HLA-DR/CD45) durchgefuhrt. Die Anzahl der positiven Reaktionen wurde unter einem Mikroskop per 0,25 mm2 gezahlt und die Korrelationen zueinander berechnet. Je nach Lokalisation in der Hornhaut (zentral, Peripherie, Limbus) waren die Langerhans-Zellen zwischen 94% (zentral) und 98% (Limbus) sowohl fur HLA-DR als auch fur das S-100-Protein positiv (p < 0,015). Das CD45-Antigen wurde in 93% (Zentrum) bis 98% (Limbus) der Falle kombiniert mit dem HLA-DR-Antigen exprimiert (p < 0,015). Unsere Untersuchungen verdeutlichen, das sowohl das S-100-Protein, als auch das CD45-Antigen in der normalen Hornhaut nur von HLA-DR-positiven Zellen exprimiert werden. Anti HLA-DR antibodies are the most commonly used antibodies to detect corneal Langerhans cells. Our study investigates the distribution of S-100 protein and CD45 compared to that of HLA-DR. Eight fresh corneas were fixed in liquid nitrogen, 4–6 μm thick sections were made and double immunostaining (HLA-DR/S-100 or HLA-DR/CD45) was performed using a double stain kit. The number of positive reactions was counted under a microscope per 0.25 mm2 and the correlation of HLA-DR expression compared to CD45 antigen or S-100 protein was analyzed. Depending on the localization of the cornea (central, periphery, limbus) Langerhans cells were positive for HLA-DR and S-100 protein in 94% (central) to 98% (limbus) (p < 0.015). The CD45 antigen was detectable in combination with HLA-DR antigen in 93% (central) to 98% (limbus) (p < 0.015). Our investigation shows, that the S-100 protein and the CD45 antigen are only expressed by HLA-DR positive cells in the normal cornea.

We describe an approach to propagating streams of information in Vehicle Ad-Hoc Networks (VANETs) based on sources of information anticipating where their information will be useful. In this paper we describe how sources can model the potential usefulness of their information using utility functions. These utility functions are converted to more compact ldquomicroutilitiesrdquo that travel with the individual data packets. The microutilities allow the information forwarding protocols to operate distributedly and independently on individual data packets, while achieving good overall coordination and delivery for entire data streams. We describe the algorithms used to convert utility functions to microutilities. Our algorithms insure that both proactive planning and reactive dropping of information in-transit are done consistent with the needs of the different applications. In this way data streams from both high priority (safety) and lower priority (traffic and commercial) applications can be propagated in the same network. We show experimental results that demonstrate the advantage of such a utility.microutility approach in serving the needs of diverse intelligent transportation system applications.

Plasminogen, the inactive precursor of plasmin, a general trypsin-like proteinase, is present at high concentration in blood and in body fluids. Most cells can recruit this proteolytic potential by secreting plasminogen activator (PA) to generate localized proteolysis in the surrounding microenvironment. PA and plasmin are serine enzymes whose pH optima match extracellular pH; further, in view of the large amount of circulating proenzyme and the broad substrate range of plasmin, the possibility that this proteolytic system can initiate a variety of proteolytic reactions or sequences should be kept in mind. PA production is precisely regulated by hormones, temporal programming, or both; and enzyme synthesis is correlated with some physiological and pathological processes requiring proteolysis. Thus PA production is coordinately regulated with ovulation, trophoblast implantation, spermatogenesis, polypeptide hormone synthesis, and some developmental phenomena; and with inflammation, tumour promotion, and neoplasia. Tissue remodelling and cell migration are common to many of these processes. Macrophage (monocyte) and polymorphonuclear leucocyte PA production is modulated by many biologically active substances. Enzyme synthesis is induced and stimulated by stimuli that recruit these cells to sites of inflammation, and it is repressed by anti-inflammatory agents, notably by glucocorticoids.

Microstructura lly clean, isov alently P-doped BaFe2As2 (Ba-122) single crystalline thin films have been prepared on MgO (001) substrates by molecular beam epitaxy. These films show a superconducting transition temperature (Tc) of over 30 K although P content is around 0.22, which is lower than the optimal one for single crystals (i.e., 0.33). The enhanced Tc at this doping level is attributed to the in-plane tensile strain. The strained film shows high transport self-field critical current densities (Jc) of over 6 MA/cm2 at 4.2 K, which are among the highest for Fe based superconductors (FeSCs). In-field Jc exceeds 0.1 MA/cm2 at m0H = 35 T for H||ab and m0H = 18 T for H||c at 4.2 K, respectively, in spite of moderate upper critical fields compared to other FeSCs with similar Tc. Structural investigations reveal no defects or misoriented grains pointing to strong pinning centers. We relate this unexpected high Jc to a strong enhancement of the vortex core energy at optimal Tc, driven by in-plane strain and doping. These unusually high Jc make P-doped Ba-122 very favorable for high-field magnet applications., 5 pages, 4 figures

We report the study of the isomeric selective OH-initiated oxidation of 1,3-butadiene in the presence of O2 and NO using the LP/LIF technique. The photolysis of monodeuterated 1-iodo-3-buten-2-ol provides only one of the possible OD-butadiene adducts, the minor addition channel product, simplifying the oxidation mechanism. We find, based on analysis of OD time-dependent traces that prompt rearrangement of initial beta-hydroxyalkyl radicals to alpha-hydroxyalkyl radicals occurs in agreement with RRKM/ME theoretical predictions. We report a rate constant of (3.3+/-1.0) x 10(-11) cm3 molecules(-1) s(-1) for deuterium abstraction from the alpha-hydroxyalkyl radical at 298 +/-2 K. Our approach demonstrates the feasibility of isomeric selective kinetic studies of the OH-initiated oxidation of unsaturated hydrocarbons.

Cyanide-catalyzed aldimine coupling was employed to synthesize compounds with 1,2-ene-diamine and alpha-imine-amine structural motifs: 1,2,N,N'-tetraphenyletheylene-1,2-diamine (13) and (+/-)-2,3-di-(2-hydroxyphenyl)-1,2-dihydroquinoxaline (17), respectively. Single-crystal X-ray diffraction provided solid-state structures and density functional theory calculations were used to probe isomeric preferences within this and the related hydroxy-ketone/ene-diol system. The ene-diamine and imine-amine core structures were calculated (B3LYP/6-311++G(d,p)) to be essentially identical in energy (DeltaG = 0.2 kcal/mol in favor of the imine-amine, within the error of the calculation). However, additional effects-such as pi conjugation-in 13 render an ene-diamine structure that is slightly more stable than the imine-amine tautomer (14) (DeltaG = 0.2-0.7 kcal/mol, within the error of the calculation). In contrast, the intramolecular hydrogen bonding present in 17 significantly favors the imine-amine isomer over the ene-diamine tautomer (18) (DeltaG = 7.2-8.9 kcal/mol). For both 13 and 17, the optimized calculated structures (B3LYP/6-31+G(d')) are identical to those observed by single-crystal X-ray diffraction.

Background: Organ culture medium and Optisol are the most commonly used corneal storage mediums. This study compares the costs for these two methods.

Cyanide-catalyzed aldimine coupling was employed to synthesize compounds with 1,2-ene-diamine and alpha-imine-amine structural motifs: 1,2,N,N'-tetraphenyletheylene-1,2-diamine (13) and (+/-)-2,3-di-(2-hydroxyphenyl)-1,2-dihydroquinoxaline (17), respectively. Single-crystal X-ray diffraction provided solid-state structures and density functional theory calculations were used to probe isomeric preferences within this and the related hydroxy-ketone/ene-diol system. The ene-diamine and imine-amine core structures were calculated (B3LYP/6-311++G(d,p)) to be essentially identical in energy (DeltaG = 0.2 kcal/mol in favor of the imine-amine, within the error of the calculation). However, additional effects-such as pi conjugation-in 13 render an ene-diamine structure that is slightly more stable than the imine-amine tautomer (14) (DeltaG = 0.2-0.7 kcal/mol, within the error of the calculation). In contrast, the intramolecular hydrogen bonding present in 17 significantly favors the imine-amine isomer over the ene-diamine tautomer (18) (DeltaG = 7.2-8.9 kcal/mol). For both 13 and 17, the optimized calculated structures (B3LYP/6-31+G(d')) are identical to those observed by single-crystal X-ray diffraction.

Thin-layer chromatography includes several steps which can be optimized. This article covers various aspects of development and validation of methods in thin-layer chromatography. Theoretical aspects concerning selection of stationary phase and mobile are described. Optimization of the mobile phase is explained through the solvent properties and explanations of several models.

Aldimine coupling (AIC) is the nitrogen analogue of the benzoin condensation and has been applied to dialdimines, providing the first examples of cyclizations effected by cyanide-catalyzed AIC. Sodium cyanide promoted the facile, intramolecular cyclization of several dialdimines in N,N-dimethylformamide, methanol, or methylene chloride/water (phase-transfer conditions) yielding a variety of six-membered heterocycles. Under aerobic conditions, an oxidative cyclization occurs to provide the diimine heterocycle. Oligomerization was observed with rigid dialdimines for which cyclization was precluded.

This paper investigates the problem of designing decentralized representations to support monitoring and inferences in sensor networks. State-space models of physical phenomena such as those arising from tracking multiple interacting targets, while commonly used in signal processing and control, suffer from the curse of dimensionality as the number of phenomena of interest increases. Furthermore, mapping an inference algorithm onto a distributed sensor network must appropriately allocate scarce sensing and communication resources. We address the state-space explosion problem by developing a distributed state-space model that switches between factored and joint state spaces as appropriate. We develop a collaborative group abstraction as a mechanism to effectively support the information flow within and across subspaces of the state-space model, which can be efficiently supported in a communication-constrained network. The approach has been implemented and demonstrated in a simulation of tracking multiple interacting targets.

The ability to characterize sensing quality is central to the design and deployment of practical distributed sensor networks. This paper introduces the concept of a sensing field defining, for each point in the physical space of a phenomenon of interest, a measure of how well a sensor network can sense the phenomenon at that point. Using target localization and tracking as examples, the paper derives an upper bound for this measure of goodness measure, using the Cramer-Rao bound and models of sensor observation and network layout. It then evaluates the validity of statistical observation models used by a family of estimators. Simulation results of applying the analytical analysis to a randomly spaced network are presented.

Polymorphism of glutathione S-transferase theta (GSTT1) modulates the toxicity of halogenated alkanes and epoxides in humans. The enzymatic activity of glutathione S-transferase theta and its corresponding gene is lacking in about 30% of the central European population. It has now been demonstrated that the background rate for sister chromatid exchange (SCE) is affected by this particular polymorphism. Smoking as a known inducer of SCE was taken into account. A group of GSTT1-positive subjects exhibited lower SCE rates than GSTT1-negative individuals (7.55 +/- 0.77 versus 8.74 +/- 1.24 SCE/mitosis, respectively, p0.005). Non-smoking GSTT1-positive individuals showed the lowest SCE rate (7.26 +/- 0.71 SCE/mitosis), significantly lower than the rates of smoking GSTT1-positive and non-smoking GSTT1-negative subjects (8.14 +/- 0.55 SCE/mitosis and 8.12 +/- 0.88 SCE/mitosis, respectively, p0.025 in both cases). Smoking GSTT1-negative subjects exhibited the highest SCE rates (9.28 +/- 1.3 SCE/mitosis). It is hypothesized that GSTT1 is protective against background genotoxic damage. Since ethylene oxide is a proven substrate of GSTT1, the detoxification of this epoxide arising from endogenous ethylene may modulate SCE background rates.

Muscles operate eccentrically to either dissipate energy for decelerating the body or to store elastic recoil energy in preparation for a shortening (concentric) contraction. The muscle forces produced during this lengthening behavior can be extremely high, despite the requisite low energetic cost. Traditionally, these high-force eccentric contractions have been associated with a muscle damage response. This clinical commentary explores the ability of the muscle-tendon system to adapt to progressively increasing eccentric muscle forces and the resultant structural and functional outcomes. Damage to the muscle-tendon is not an obligatory response. Rather, the muscle can hypertrophy and a change in the spring characteristics of muscle can enhance power; the tendon also adapts so as to tolerate higher tensions. Both basic and clinical findings are discussed. Specifically, we explore the nature of the structural changes and how these adaptations may help prevent musculoskeletal injury, improve sport performance, and overcome musculoskeletal impairments.

Summary form only given. The article describes a state-centric abstraction for application users to interact with sensor networks. Just as in data-centric routing and storage where physical nodes are less important than the data itself, state-centric abstraction introduces "states" as a natural vocabulary to describe spatio-temporal physical phenomena that the sensor networks are typically designed for. Application programmers specify the computation as creation, sharing and transformation of states, which naturally map to descriptions in signal processing and control applications. We argue that due to the dynamic nature of sensor networks, programs written in state-centric abstractions are more invariant to constant changes in data stream configurations and make the resulting software more portable across multiple sensor network platforms. With the help of models of sensor collaboration, sensing and estimation, the state-centric specifications are mapped into collaborative processing tasks at compile time, and further maintained at run time, leveraging the data-centric caching and routing services. We use a multi-target tracking system as an example to show how state-centric programming models can raise the abstraction level for users to interact with sensor networks and help modularize the design.

The control of real time, nonlinear, large-scale systems - systems with large aggregations of sensors and actuators - is seldom explored in actual operating physical systems. In such many-element systems, control issues such as actuation allocation, fusion of sensor data, and system identification emerge as challenging problems for large-scale system control. In this work, constrained optimization is used to solve these problems as applied to the control of an object moving system with 1,152 actuators and 32,000 sensors with a 2 ms control loop time. Solutions for allocating actuation among large numbers of actuators using hierarchical constrained optimization and fusing the output of many sensors into a small number of final measurements under tight real time constraints have been developed. This paper demonstrate that hyper-redundant systems are capable of system self-identification, and that constrained optimization can effectively solve problems associated with control of many-element systems.

This paper presents a framework for modeling faults in hybrid systems that leads to an efficient approach for monitoring and diagnosis of real-time embedded systems. We describe a fault parameterization based on hybrid automata models and consider both abrupt failures and gradual degradation of system components. Our approach also addresses the computational problem of coping with large amount of sensor data by using a discrete event model of the system so as to focus distributed signal analysis on when and where to look for signatures of interest. The approach has been demonstrated for the online diagnosis of a hybrid system, the Xerox DC265 printer.

This paper presents a class of signal processing techniques for collaborative signal processing in ad hoc sensor networks, focusing on a vehicle tracking application. In particular, we study two types of commonly used sensors—acoustic-amplitude sensors for target distance estimation and direction-of-arrival sensors for bearing estimation—and investigate how networks of such sensors can collaborate to extract useful information with minimal resource usage. The information-driven sensor collaboration has several advantages: tracking is distributed, and the network is energy-efficient, activated only on a when-needed basis. We demonstrate the effectiveness of the approach to target tracking using both simulation and field data.

The tradeoff between performance and scalability is a fundamental issue in distributed sensor networks. In this paper, we propose a novel scheme to efficiently organize and utilize network resources for target localization. Motivated by the essential role of geographic proximity in sensing, sensors are organized into geographically local collaborative groups. In a target tracking context, we present a dynamic group management method to initiate and maintain multiple tracks in a distributed manner. Collaborative groups are formed, each responsible for tracking a single target. The sensor nodes within a group coordinate their behavior using geographically-limited message passing. Mechanisms such as these for managing local collaborations are essential building blocks for scalable sensor network applications.

Many lasers are available today for clinical application in dentistry. For the removal of caries in enamel or dentin only few lasers can be used. Er:YAG lasers have a wavelength that coincides with the absorption maximum of water. Because of this characteristic the ablative effect in enamel and dentin is high and these lasers can be used beneficially for caries removal and small preparations. The possible side effects of Er:YAG lasers with water-cooling are minor compared to those of rotary instruments. A pulpal reaction will occur only if there is a very thin dentin layer over the pulp or direct application of the laser beam to the pulp. There is no increased heating with the Er:YAG laser so with vital teeth there is a positive reaction by the formation of reparative dentin. To the existing indications for lasers like caries removal and preparation of small cavities, in the future new techniques can be added like the use of dyes for enhancement of absorption of the laser radiation, and minimal invasive techniques using feedback from a system for caries detection.

SUMMARYDuring normal animal movements, the forces produced by the locomotor muscles may be greater than, equal to or less than the forces acting on those muscles, the consequences of which significantly affect both the maximum force produced and the energy consumed by the muscles. Lengthening (eccentric)contractions result in the greatest muscle forces at the lowest relative energetic costs. Eccentric contractions play a key role in storing elastic strain energy which, when recovered in subsequent contractions, has been shown to result in enhanced force, work or power outputs. We present data that support the concept that this ability of muscle to store and recover elastic strain energy is an adaptable property of skeletal muscle. Further, we speculate that a crucial element in that muscle spring may be the protein titin. It too seems to adapt to muscle use, and its stiffness seems to be`tuned' to the frequency of normal muscle use.

The purpose of this double-blind, prospective, latin-square crossover randomised study was to examine the efficacy of a mouthrinse solution containing a combination of triclosan, amine fluoride and sodium fluoride on supragingival plaque regrowth compared to a placebo and a chlorhexidine solution.12 volunteers refrained after professional oral prophylaxis from all mechanical hygiene measures for the following 96 h and rinsed instead cross-over in a randomised order with either chlorhexidine digluconate (0.2%, positive control), a verum solution (0.5% amine fluoride, 0.028% sodium fluoride, 0.03% triclosan) or a placebo solution. The plaque index was assessed after 24 and 96 h (PI 1, PI 2) and the plaque area (PA) photographed and calculated after 96 h of undisturbed plaque regrowth. Moreover plaque samples were taken after 24 and 96 h and examined with the vital fluorescence technique to assess the vitality of the biofilm microbiota (VF 1, VF 2).The amine-fluoride-triclosan solution reduced the clinical parameters (PI and PA) as well as the vitality of the plaque flora (VF) significantly when compared to the placebo solution. The verum reached a 36.5% (p0.05) and a 39.8% reduction (p0.0001) in PI 1 and PI 2, respectively, concomittant with a reduction of 23.8% and 32.2% (p0.001) in VF 1 and VF 2 and of 46.9% (p0.0001) in PA at day 4. This was less than the reductions found with the positive control, i.e., the 0.2% chlorhexidine solution (54.2% and 71.1% reduction in PI 1 and PI 2, 40.0% and 53.4% in VF 1 and VF 2 and 71.5% in PA). However, significant differences between both active solutions were only established for PI 2 and PA.During 4-day plaque regrowth the amine-fluoride-triclosan product displayed a significant antibacterial and plaque-reducing action in comparison to the control.

Oxidant stress and peroxidation of lipids have been implicated in the pathogenesis of a variety of human diseases’-7. A highly accurate marker of oxidative stressin vivois the quantification of isoprostanes8’9. Isoprostanes (IsoPs) are prostaglandin (PG)-like compounds generated from the free radical catalyzed peroxidation of arachidonic acid. The generation of these compounds proceeds through bicyclic endoperoxide PGH2-like intermediates which are subsequently reduced to PGF2-like compounds (F2-IsoPs)10or undergo rearrangement to PGD2and PGE2-like compounds (D2/E2- IsoPs)’1and thromboxane-like compounds (isothromboxanes)12.In vivo, arachidonic acid is primarily present esterified to phospholipids. Thus, IsoPs are formed esterifiedin situand are subsequently released preformed13. In addition to being reliable markers of oxidative stressin vivo, several of the IsoPs have been shown to be capable of exerting potent biological activity; therefore, these compounds may mediate some of the pathophysiological sequelae of oxidant stress“.

The recently introduced Er:YAG laser seems to be a promising alternative in periodontal treatment due to its thermo-mechanical ablation mechanism. The present study attempted to compare the effects of an Er:YAG laser on periodontally involved root surfaces at different power settings in vivo and in vitro using scanning electron microscopic (SEM) observations.Forty single rooted teeth (160 surfaces), with advanced periodontal destruction that were scheduled for extraction, were divided into two groups of 80 each which were treated in vivo (group A) and immediately after extraction in vitro (group B) using one of the following energy settings: 120, 140, 160, and 180 mJ at 10 Hz (71, 83, 94, and 106 J/cm(2)/pulse). The morphological changes on the treated root surfaces were evaluated using scanning electron microscopic (SEM) observations to assess the laser induced ultrastructural changes. The severity of the changes was evaluated according to an arbitrary scale in 7 degrees [1-7]. Untreated peripheral areas served as control.All surfaces treated in vitro (group B) showed visible crater-like defects with notch-edged borders. The depth of the surface damages varied with the power applied and was localized into cementum at energy settings of 120-160 mJ but also reached dentine at 180 mJ. Compared to that, all in vivo (group A) treated surfaces showed a homogeneous and smooth root surface morphology. The surface alterations were not related to the used energy setting.The results of the present study showed that the clinical use of an Er:YAG laser resulted in a smooth root surface morphology, even at higher energy settings. The results also seem to indicate that calculus removal can be selectively done in vivo.

When the force applied to a muscle exceeds the force produced by the muscle it will lengthen, absorbing mechanical energy. These eccentric contractions, which result in both braking and storing elastic recoil energy in normal locomotion, require very little metabolic energy, yet they are characterized by high force production.

The pattern of cytokeratin expression has been extensively described in the normal and inflamed periodontium. However, there is no information regarding the pattern of cytokeratin expression in the periodontium which has been reformed following regenerative periodontal surgery. The aim of the present investigation was to evaluate the pattern of cytokeratin expression in the reformed human and monkey periodontium following regenerative and conventional periodontal surgery. In 3 monkeys, acute fenestration-type and chronic intrabony defects were treated with guided tissue regeneration (GTR), enamel matrix proteins (EMD), or coronally repositioned flap surgery (control). After a healing period of 5 months, the animals were sacrificed and perfused with 10% buffered formalin for fixation. Specimens containing the defects and surrounding tissues were dissected free, decalcified in EDTA and embedded in paraffin. Histological sections were cut with the microtome set at 3 microm. The sections were alternatively stained either with hematoxylin and eosin, or immunohistochemically by using one of the broad range monoclonal antibodies 34betaE 12 (for cytokeratins 1, 5, 10 and 14) or KL 1 (for cytokeratins 1, 2, 5, 6, 7, 8, 10, 11, 16 and 19), or one of the individual monoclonal antibodies LL025 (for cytokeratin 16), DC 10 (for cytokeratin 18), A53-B/A2 (for cytokeratin 19). Twelve patients, each displaying one deep intrabony defect scheduled for extraction due to advanced periodontitis or prosthetic reasons, were treated as described above. Following a healing period of 6 months, the teeth were extracted together with some of their surrounding soft and hard tissues. The histological and immunohistochemical processing of the human biopsies was identical to that described in monkeys. The results revealed that both the normal non-treated (original) monkey and human junctional epithelium stained strongly with all of the monoclonal antibodies used. The reformed junctional epithelium displayed the same cytokeratin expression pattern as the non-treated junctional epithelium. No differences regarding the cytokeratin expression pattern of the junctional epithelium were found between the treatments and types of healing (i.e. regenerative, through a new periodontal ligament, or reparative through a long junctional epithelium). In the intact periodontal ligament, the epithelial rests of Malassez displayed a comparable cytokeratin expression pattern to that of the junctional epithelium. However, no expression of cytokeratins was seen in the newly formed periodontal ligament. In such specimens, cytokeratin expression was observed only until the borderline between the regenerated cementum and the epithelial downgrowth. It was concluded that: a) the reformed junctional epithelium, following any type of surgical procedure, displays a similar pattern of cytokeratin expression to the original junctional epithelium; b) in the newly formed periodontal ligament, no expression of cytokeratins is present; and c) the epithelial rests of Malassez do not seem to reform after regenerative periodontal surgery.

Many products that claim to have caries-preventive effects are available to the general public. Different and sometimes even contradictory claims of effectiveness are given in advertisements. Dentists would like to give good information and advice to their patients and the public with respect to the efficacy of different products. In medicine and dentistry there are guidelines which consider the ethics, design, conduct as well as the recording and reporting of clinical studies. The effects of clinical trials in subjects with a high risk for caries or in a population with a high caries prevalence are not comparable to the situation in most industrialized countries today. The sample should, therefore, include subjects with a range of prevalences and of risks for a valid assumption of efficacy. Reliable data can be obtained if a strict control of the design and management of the clinical trials is maintained over the duration of the experiment. New diagnostic methods used in the study must be validated and could lead to trials of a shorter duration. For a new fluoride product the bioavailability of the ion should be demonstrated. The results must have the necessary characteristics to be included in a meta-analysis. Thesis: The caries-preventive effect of a fluoride product cannot be accurately measured in populations with a variety of preventive products in use. On the other hand, the measured effect in populations with caries prevalence higher than those of the country where it will be used is not comparable to the real situation.

Different sensory systems (e.g. proprioception and vision) have a combined influence on the perception of body orientation, but the timescale over which they can be integrated remains unknown. Here we examined how visual information and neck proprioception interact in perception of the "subjective straight ahead" (SSA), as a function of time since initial stimulation. In complete darkness, healthy subjects directed a laser spot to the point felt subjectively to be exactly straight ahead of the trunk. As previously observed, left neck muscle vibration led to a disparity between subjective perception and objective position of the body midline, with SSA misplaced to the left. We found that this displacement was sustained throughout 28 min of continuous proprioceptive stimulation, provided there was no visual input. Moreover, prolonged vibration of neck muscles leads to a continuing disparity between subjective and objective body orientation even after offset of the vibration; the longer the preceding vibration, the more persistent the illusory deviation of body orientation. To examine the role of vision, one group of subjects fixated a central visual target at the start of each block of continuous neck vibration, with SSA then measured at successive intervals in darkness. The illusory deviation of SSA was eliminated whenever visual input was provided, but returned as a linear function of time when visual information was eliminated. These results reveal: the persistent effects of neck proprioception on the SSA, both during and after vibration; the influence of vision; and integration between incoming proprioceptive information and working memory traces of visual information.

Children and adolescents must be examined often for occlusal caries. Diagnosis of fissure caries is difficult especially when the tooth surface appears seemingly intact. It has been shown that using traditional clinical methods, as little as 20% of teeth with fissure caries under intact surfaces were correctly recognised as such. Therefore, new methods for increasing the accuracy of diagnosis have been sought for years. Recently, a new device, based on fluorescence measurements, was introduced. The purpose of this study was to test the device under in vivo conditions in order to provide recommendations for its use in the dental office. Seven general dental practitioners examined a total of 332 occlusal surfaces in 240 patients. Caries extent was determined for each site after operative intervention (='gold standard'). Clinical inspection and analysis of bitewing radiographs exhibited statistically significant lower sensitivities (31-63%) than did the DIAGNOdent device (sensitivityor = 92%). It is recommended that the laser device is used in the decision-making process in relation to the diagnosis of occlusal caries as a second opinion in cases of doubt after visual inspection.

The aim of this laboratory study was to evaluate the marginal sealing ability of four glass-ionomer cements in cervical restorations (Class V) using dye penetration. Two conventional (C-GIC) and two resin-modified (RM-GIC) cements were used either with or without dentin conditioning with polyacrylic acid.96 cervical cavities of standardized size were prepared in vitro in the vestibular and lingual portions at the cementoenamel level of 48 premolars. The coronal margins were prepared in enamel, the apical margins were localized in dentin. The 96 cavities were randomly divided into 4 groups of n = 24 each. The cavities of each group were filled with one of the test materials, and only half of the cavities received a dentin conditioning for 20 s with polyacrylic acids before filling. The fillings were finished with a set of abrasive disks 24 h after setting. The restored teeth were stored in saline for 4 weeks and subjected to dye penetration. The depth of dye penetration along the coronal and apical margin was measured on 4 longitudinal sections of each tooth with a semi-automatic image analysis system at 40x magnification.Mean depth of dye penetration ranged from 0 (ChemFil superior without conditioner) to 0.13 mm (ChemFil superior with conditioner) at enamel sites, and from 0.02 (Fuji II LC with conditioner) to 0.74 mm (ChemFil superior with and without conditioner) at dentin sites.The conditioning of the cavities before filling improved the marginal adaptation significantly only in the Ketac-Fil group. Conventional glass-ionomer cements (C-GIC) in general demonstrated a lower sealing ability than the light-activated, resin-modified cements (RM-GIC). The adaptation of Photac-Fil quick is best without pretreatment--as recommended by the manufacturer.

To examine the spatial structure of dental biofilms a vital fluorescence technique was combined with optical analysis of sections in a confocal laser scanning microscope (CLSM). Enamel slaps were worn in intraoral splints by three volunteers for five days to accumulate smooth-surface plaque. After vital staining with fluorescein diacetate and ethidium bromide the specimens were processed for CLSM examination. Optical sections 1 microm apart were analysed in the z-axis of these dental biofilms. One of the films was 15 microm high, sparse and showed low vitality, i.e.16%, while the others were taller (25 and 31 microm) and more vital, i.e. up to 30 and 69%, respectively. In all instances the bacterial vitality increased from the enamel surface to the central part of the plaque and decreased again in the outer parts of the biofilm. The spatial arrangement of the microorganisms in the biofilm showed voids outlined by layers of vital bacteria, which themselves were packed in layers of dead material.

The aim of the present study was to evaluate histologically in monkeys the healing in acute fenestration-type defects following treatment with guided tissue regeneration (GTR) or enamel matrix proteins (EMD). Standardized "critical size" fenestration-type defects were produced surgically at the vestibular aspect of teeth 13, 23, 33, 43 in three monkeys (Macaca fascicularis). The vestibular bone plates were removed and the root surfaces were debrided by means of hand instruments in order to completely remove the root cementum. Following root conditioning with ethylenediaminetetraacetate (EDTA), the defects were treated using one of the following therapies: (1) GTR, (2) EMD, or (3) control (coronally repositioned flaps). After 5 months the animals were killed and perfused with 10% buffered formalin for fixation. Specimens containing the defects and surrounding tissues were dissected free, decalcified in EDTA, and embedded in paraffin. Eight-micrometer-thick step serial histological sections were cut in a vestibulo-oral direction, stained with hematoxylin and eosin or oxone-aldehyde-fuchsin-Halmi, and subsequently examined under the light microscope. The results showed that, in the defects treated with GTR, a new connective tissue attachment (i.e., new cementum with inserting collagen fibers) and new bone formation had consistently occurred, whereas, in the defects treated with EMD or with coronally repositioned flaps, new attachment and new bone reformed to a varying extent. The quality of the cementum did not differ after EMD, GTR, or flap surgery. It was concluded that GTR treatment with bioresorbable membranes seems to predictably promote new attachment and new bone formation, whereas the application of EDTA or EMD may also enhance periodontal healing to a certain extent. Further studies with higher numbers of animals and defects are needed in order to definitely clarify the effect of root surface conditioning with EDTA and EMD on periodontal healing.

Isoprostanes (IsoP) are produced exclusively from free radical damage to arachidonic acid, a fatty acid that is evenly distributed throughout white matter and gray matter, whereas neuroprostanes (NPs) are generated analogously from docosahexaenoic acid (DHA), a fatty acid enriched in gray matter where it is concentrated in neurons. IsoP and NPs derive from endoperoxide intermediates that isomerize to D/E-ring forms or that are reduced to F-ring compounds. We quantified F-ring and D/E-ring IsoP and NPs in temporal and parietal cortex, hippocampus, and cerebellum of nine definite Alzheimer’s disease (AD) patients and 11 age-matched controls. Total NP levels (F-ring plus D/E-ring), but not total IsoP, were significantly greater in AD than controls (P < 0.0001); only cerebral regions in AD patients had NPs greater than controls (P < 0.05). The F-ring to D/E-ring ratio for NPs, but not IsoP, was 40 to 70% lower in all brain regions of AD patients compared to controls (P < 0.005). These data extend results from in situ techniques, that have localized reactive products of lipid peroxidation primarily to neurons, by quantifying significantly greater free radical damage to the DHA-containing compartments in cerebrum in AD patients than controls, and suggest that one mechanism of increased oxidative stress may be diminished reducing capacity in DHA-containing compartments.

Numerous in vitro cell culture, animal, and tissue homogenate studies have implicated free radical damage in the pathogenesis of Alzheimer’s disease (AD) 1,2. A variety of methods have been employed to assess the free radical damage; however, most of these methods suffer from either non-quantitative results, a lack of information about cellular localization, or both3- 10.

Case-control studies are subject to the problem of population stratification, which can occur in ethnically mixed populations and can lead to significant associations being detected at loci that have nothing to do with disease. Here, we describe a way to measure and correct for stratification by genotyping a moderate number of unlinked genetic markers in the same set of cases and controls in which a candidate association was found. The average of association statistics across the markers directly measures stratification. By dividing the candidate association statistic by this average, a P-value can be obtained that corrects for stratification.

Progression to androgen independence remains the main problem that impacts on survival and quality of life in prostate cancer patients. We have investigated the potency of tributyrin, an orally available prodrug of butyrate, to induce growth arrest, differentiation and apoptosis in LNCaP, PC-3 and TSU-PR1 human prostate cancer cell lines. Cells were treated with 0.1 to 5 mM tributyrin or sodium butyrate. Growth inhibition, cell cycle arrest and apoptosis induction was assessed using standard methods. Both agents induced a more differentiated, fibroblast-like phenotype in androgen-sensitive as well as androgen-resistant cell lines. Expression of prostate-specific antigen was increased in LNCaP cells by tributyrin as a indicator of differentiation. The IC(50) for sodium butyrate was 2.5 mM in PC-3 and TSU-PR1 cells. LNCaP cells exhibited50% growth inhibition at 5 mM sodium butyrate. However, the IC(50) for tributyrin was 0.8 mM in PC-3 cells, 1.2 mM in TSU-PR1 cells and 3.1 mM in LNCaP cells. Flow cytometry revealed a strong G1-arrest after exposure to tributyrin or sodium butyrate. Both agents resulted in a strong increase of apoptosis rates compared with mock-treated cells. Overall, tributyrin had a 2.5- to 3-fold growth inhibitory and apoptosis-inducing potency compared with equimolar concentrations of sodium butyrate. Our results demonstrate that tributyrin is more potent than butyrate in regard to cell growth inhibition and apoptosis induction at pharmacologically relevant concentrations. Hence, tributyrin may be a promising candidate for clinical protocols in prostate cancer.

The motion of human scale objects requires MEMS-like device arrays capable of providing reasonable forces ($GTR mN) over human scale distances (10-100 cm). In principle batch fabricated values controlling air jets can satisfy these actuation requirements. By extending printed circuit board technology to include electromechanical actuation, analogous to the extension of VLSI to MEMS, the requirement of low system cost can be achieved through batch fabrication and integration of the transduction elements with computational and communication elements. In this paper we show that modulated air jets arrayed with position sensors can support and accelerate flexible media without physical contact. Precise motion control with three degrees of freedom parallel to the array, using high flow, low pressure air jet arrays is enabled using electrostatic valves having opening and closing times of approximately equals 1 ms. We present results of an exemplary platform based on printed circuit board technologies, having an array of 576 electrostatic flap valvves (1152 for double-sided actuation) and associated oriented jets, and an integrated array of 32,000 optical sensors for high resolution detection of paper edge positions. Under closed loop control edge positioning has a standard deviation of approximately equals 25 microns. Fabrication and control of the system is described.

The aim of the present study was to evaluate clinically and histologically the treatment of intrabony periodontal defects with a bioresorbable membrane barrier. Fifty-two intrabony periodontal defects were treated according to the principles of guided tissue regeneration (GTR) with a bioresorbable membrane. Results were evaluated by assessing probing pocket depth, recession of the gingival margin, and clinical attachment level at baseline and at 1 and 2 years after therapy. Bone level changes were evaluated radiographically. The postoperative phase was uneventful in all cases. There was a mean probing pocket depth reduction from 8.4 to 3.6 mm, a mean increase of gingival margin recession from 1.5 to 3.0 mm, and a mean clinical attachment level change from 9.9 to 6.5 mm. Mean attachment gain was 3.4 mm. Two teeth scheduled for extraction were also treated with the same bioresorbable membrane. The histologic analysis 6 months after treatment revealed the formation of new connective tissue attachment and new alveolar bone in both cases. Based on the histologic findings it can be concluded that the clinical improvements following GTR with this type of bioresorbable membrane may represent, at least in part, true periodontal regeneration.

Enamel matrix proteins, including Emdogain, have been proposed as a new modality for regenerative periodontal treatment. However, limited information is available concerning the clinical applicability and therapeutic results with Emdogain. The aim of the present study was therefore to evaluate the clinical outcome following the application of Emdogain in the treatment of intrabony periodontal defects. Twenty-eight patients with marginal periodontitis (thirty-two 2- and 3-walled intrabony defects) were included in this study. The following parameters were evaluated prior to treatment and 8 months after treatment: probing pocket depth, recession of the gingival margin, and clinical attachment level. The postoperative healing phase was uneventful in all cases. There were no complications such as allergic reactions, abscess formation, or infections throughout the entire study period. The mean probing pocket depth was reduced from 8.7 +/- 1.5 mm at baseline to 4.3 +/- 1.6 mm after 8 months (P0.001), the mean gingival recession increased from 1.8 +/- 1.2 mm to 3.3 +/- 0.9 mm, and the mean clinical attachment level changed from 10.6 +/- 1.9 mm to 7.6 +/- 1.8 mm (P0.001). New hard tissue formation was radiographically observed in 26 of the 32 defects. The present results suggest that the treatment of intrabony periodontal defects with Emdogain may lead to significant improvements of all of the investigated clinical parameters. However, controlled histologic and clinical trials are needed to compare this treatment modality with other conventional and regenerative periodontal surgical methods.