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A low-cost medium for the amylolytic lactic acid bacterium, Enterococcus faecium DMF78, was developed by response surface methodology using sago starch as substrate. Six nitrogen sources, wort, whey, corn steep liquor (CSL), trub, soybean and mungbean flour were evaluated to completely eliminate or dramatically reduce the use of expensive yeast extract, beef extract, and proteose peptone in the medium. Highest lactic acid yield was achieved using a medium with 35.0% whey, 25.0% wort, 3.0% diluted CSL and 0.076% proteose peptone in the standard formulation of de Mann, Rogosa and Sharpe (MRS) medium. Validation run of the optimized medium in the 2-L fermentor revealed slightly lower starch utilization (0.75 g/g) and low cell growth, but high lactic acid yield of 28.83 g/L after 24 hours, as compared to fermentation using complete sago-MRS medium. [ABSTRACT FROM AUTHOR]

In an effort to lower the cost of raw-starch-digesting amylase (RSDA) production for E. faecium DMF78, statistical tools were used to optimize production parameters. Plackett-Burman and Box-Behnken has been carried out to evaluate the effects of 17 different parameters and conditions. Proteose peptone, beef extract, and MRS salts were found to be positive significant effectors for amylase production. On the other hand, yeast extract, sodium dodecyl sulfate (SDS), and corn steep liquor (CSL) were negative effectors. The optimized medium resulted to a 466.67% increase in amylase activity and 41.99% decrease in cost compared to the modified MRS medium with the elimination of yeast extract, trub, SDS, and CSL. [ABSTRACT FROM AUTHOR]

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Copyright of Revista Facultad de Odontología Universidad de Antioquia is the property of Universidad de Antioquia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: To assess the nutritional status of orthopaedic patients. DESIGN: Prospective observation study. SETTING: Tertiary hospital, Clinical Nutrition Unit. PATIENTS: 107 patients (56.1% women, 43.9% men, 62.1 +/- 20.4 years) are studied at admission to the orthopaedic unit and it is observed the evolution of orthopaedic surgery patients with hip or knee prosthesis. MATERIAL AND METHODS: Medical history, anthropometric data and three nutritional screening tools were considered: Mini-Nutritional Assessment (MNA), Malnutrition Universal Screening Tool (MUST) y Valoración Global Subjetiva (VGS). It was assessed the prevalence of malnutrition in patients on admission to hospital, using different methods and determining if a correlation exists between malnutrition and other factors, such as the development of pressure sores of the dependence in activities of daily living. RESULTS: The risk of malnutrition is 22 and 24/100 patients admitted at hospital, if MNA and SGA are used, respectively (SGA caregorized 4% of the group as 'malnourished'). 80% patients are considered to be at high risk of malnutrition using MUST. 1.7% patients presented a Body Mass Index (BMI) lower than 18.5. There is a strong agreement between malnutrition and neropsychological status (p = 0.001), and the dependence in activities of daily living (p = 0.002), and between medium and high risk of malnutrition and the development of pressure sores (p = 0.003). If MNA and SGA (p = 0.000) are used, post-surgical patients presents a deteriorating nutritional status. CONCLUSIONS: The prevalence of malnutrition in patients on admission to hospital is elevated. Orthopaedic surgery patients with hip and knee prosthesis presented a deteriorating nutritional status, and a higher incidence of pressure sores in a month. [ABSTRACT FROM AUTHOR]

Objective: To assess the nutritional status of orthopaedic patients. Design: Prospective observation study. Setting: Tertiary hospital, Clinical Nutrition Unit. Patients: 107 patients (56,1% women, 43,9% men, 62,1 ± 20,4 years) are studied at admission to the orthopaedic unit and it is observed the evolution of orthopaedic surgery patients with hip or knee prosthesis. Material and methods: Medical history, anthropometric data and three nutritional screening tools were considered: Mini-Nutritional Assessment (MNA), Malnutrition Universal Screening Tool (MUST) y Valoración Global Subjetiva (VGS). It was assessed the prevalence of malnutrition in patients on admission to hospital, using different methods and determining if a correlation exists between malnutrition and other factors, such as the development of pressure sores of the dependence in activities of daily living. Results: The risk of malnutrition is 22 and 24/100 patients admitted at hospital, if MNA and SGA are used, respectively (SGA caregorized 4% of the group as "malnourished"). 80% patients are considered to be at high risk of malnutrition using MUST. 1,7% patients presented a Body Mass Index (BMI) lower than 18,5. There is a strong agreement between malnutrition and neropsychological status (p = 0,001), and the dependence in activities of daily living (p = 0,002), and between medium and high risk of malnutrition and the development of pressure sores (p = 0,003). If MNA and SGA (p = 0,000) are used, post-surgical patients presents a deteriorating nutritional status. Conclusions: The prevalence of malnutrition in patients on admission to hospital is elevated. Orthopaedic surgery patients with hip and knee prosthesis presented a deteriorating nutritional status, and a higher incidence of pressure sores in a month. [ABSTRACT FROM AUTHOR]