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107. Assessment of Knowledge, Attitude and Practices and the Analysis of Risk Factors regarding Schistosomiasis Among Fishermen and Boatmen in the Dongting Lake Basin, the People's Republic of China

Author

Guan, Zhou, primary, Dai, Si-Min, additional, Zhou, Jie, additional, Ren, Xiao-Bing, additional, Qin, Zhi-Qiang, additional, Li, Yin-Long, additional, Lv, Shan, additional, Li, Shi-Zhu, additional, Zhou, Xiao-Nong, additional, and Xu, Jing, additional

Published
2020
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109. [Analysis of schistosomiasis cases report in National Notifiable Disease Report System in China, 2015-2017]

Author

Dai, Si-Min, Xu, Zhi-Min, Zhang, Li-Juan, Xu, Jing, and Lü, Shan

Subjects
Adult, China, Sex Factors, Risk Factors, Age Factors, Schistosoma haematobium, Animals, Humans, Schistosomiasis, Schistosoma mansoni, Middle Aged, Disease Notification
Abstract

To understand the reporting situation of schistosomiasis cases in National Notifiable Disease Report System (NNDRS) in China from 2015 to 2017, and to seek current deficiencies on case reporting as well as to analyze the epidemiological characteristics of schistosomiasis cases.The data of schistosomiasis cases in China from 2015 to 2017 were collected from NNDRS, and the reporting situation and epidemiological characteristics of schistosomiasis cases were analyzed.From 2015 to 2017, totally 59 981 schistosomiasis cases were reported in China, among which, 1 460 cases were deleted, and 58 521 were censored cases. The statistics and analysis showed that a part of the case reporting had been carried out in nonstandard ways, mainly involving the random deletion of cases, reporting time not compliance with regulations, incorrect classification, and severe omission of cases. Among the 58 521 censored cases, the sex ratio of the male to the female was 1.83∶1, the average age of the cases was (51.91 ± 11.30) years, and farmers and fishermen accounted for 93.26% (54 577 cases) and 3.46% (2 022 cases), respectively. The reported cases mainly concentrated in Anhui, Hunan, Hubei and Jiangxi provinces, accounting for 99.73% of the total number in China. During this period, Beijing, Zhejiang and other provinces (cities and regions) reported 11 imported schistosomiasis cases, all of them were schistosomiasis mansoni cases or schistosomiasis haematobia cases.From 2015 to 2017, the reported cases of schistosomiasis are mainly clinically diagnosed cases. Compared with the annual report of the national schistosomiasis control, the number of confirmed cases in NNDRS is seriously missed. Therefore, the endemic provinces should strengthen the supervision on confirmed cases and reporting quality of schistosomiasis cases in accordance with the relevant law and regulation.[

Published
2019

110. [Progress of research on association between schistosomiasis and single nucleotide polymorphisms]

Author

Dai, Si-Min, Qin, Zhi-Qiang, Lü, Chao, and Xu, Jing

Subjects
Haplotypes, Research, Humans, Schistosomiasis, Genetic Predisposition to Disease, Polymorphism, Single Nucleotide, Alleles
Abstract

Single nucleotide polymorphisms (SNP) has been regarded as a new genetic marker due to its rich dynamics and relatively stable heredity. SNP can express the relationship between genes and diseases to seek disease-linked genes particularly. A number of domestic and international studies have demonstrated that the formation of schistosomiasis egg granuloma is closely linked to HLA-Ⅱ related antigen. By detecting alleles loci of HLA-Ⅱ gene, researchers can identify the gene or haplotype that is associated with the susceptibility and progression of schistosomiasis. The detected candidate genes can serve useful therapeutic and preventative purposes by providing new technical methods to defeat schistosomiasis. In-depth study on SNP is beneficial to evaluate schistosomiasis inclination clinically, as well as to promote early diagnosis and preventive treatment.[

Published
2019

112. Effects of replacement of alfalfa by big-leaf mulberry on growth performance, digestion and meat quality in growing rabbits

Author

National Natural Science Foundation of China, Wu, Xue Zhuang, Yang, Pei Long, Gao, Xiu Hua, Wen, Zhi Guo, Dai, Si Fa, Zhu, Ming Xia, Wang, Li Xin, National Natural Science Foundation of China, Wu, Xue Zhuang, Yang, Pei Long, Gao, Xiu Hua, Wen, Zhi Guo, Dai, Si Fa, Zhu, Ming Xia, and Wang, Li Xin

Abstract

[EN] This study aimed to investigate the effect of sundried big-leaf mulberry (BLM) as a replacement for alfalfa on the growth performance, digestibility of nutrients, nitrogen (N) utilisation and meat quality in New Zealand White rabbits. One hundred and sixty weaned rabbits, aged 35±1 d and with a body weight of 755±26 g, were randomly assigned to the 4 treatments with 20 replicates of 2 rabbits (1 male and 1 female) each. Animal performance was evaluated between the 35th and 77th d of age in 40 animals per treatment. The coefficients of total tract apparent digestibility (CTTAD) of nutrients and N utilisation were measured between 77 to 83 d of age in 30 animals per treatment. The control rabbits were fed a corn-soybean meal-alfalfa meal based diet. The three experimental groups were fed a similar diet in which the alfalfa meal was replaced by 10% (BLM10), 20% (BLM20), or 30% (BLM30) BLM. The final body weights and average daily gain were higher (P<0.05) in BLM20 rabbits compared to c

Published
2019

113. Dib ligand effects on ZnII coordination polymers built from a semirigid tricarboxylate ligand: Topological structures and luminescence properties

Author

Jia-Cheng Liu and Chang-Dai Si

Subjects
Steric effects, 010405 organic chemistry, Chemistry, Ligand, Supramolecular chemistry, Stacking, 010402 general chemistry, Ring (chemistry), Topology, 01 natural sciences, Tricarboxylate, 0104 chemical sciences, Inorganic Chemistry, Materials Chemistry, Physical and Theoretical Chemistry, Luminescence, Topology (chemistry)
Abstract

Four complexes based on H3L, namely, [Zn2(L)(dib)1.5(μ2-O)]n (1), {[Zn(HL)(dib)]·H2O}n (2), [Zn(HL)(dib)]n (3), [Zn3(L)2(dib)3]n (4) [4-[(3-carboxynaphthalen-2-yl)oxy]phthalic acid (H3L) and 1,4-di(1H-imidazol-1-yl) benzene (dib)] have been synthesized by solvothermal reactions. Complex 1 displays (3,5)-connected gra-type topology with a Schlafli symbol of (63)(69.8), the two-dimensional (2D) structure was extended to a three-dimensional (3D) framework by dib ligand, showing an enhanced luminescence intensity. Complex 2 possesses a 2D 4-connected sql-type topology with a Schlafli symbol of (44.62), which are further stacked with each other by C–H⋯π and C–H⋯O interactions to form a 3D supramolecular framework. Complex 3 features a novel 4-connected topology with a Schlafli symbol of (33.42.5), the driving forces in 3D supramolecular​ frameworks are C–H⋯O, C–H⋯π, and π⋯π stacking interactions in 3. Complex 4 has a 3D (3,4,4)-connected net with a Schlafli symbol of (63.8.102)2(63)2(64.8.10), representing a new topological prototype. In addition, complexes 1–4 exhibit non-interpenetrated structures owing to the steric hindrance of the naphthalene ring. It is also noteworthy that dib Ligand may not only affect the coordination mode of polycarboxylate acid but also subtly adjust the pH value of reaction solvent as well as N,N′-dimethylformamide (DMF). Additionally, the luminescent properties were investigated in the solid state at room temperature for 1–4.

Published
2016
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114. A series of Zn/Cd coordination polymers constructed from 1,4-naphthalenedicarboxylate and N-donor ligands: Syntheses, structures and luminescence sensing of Cr3+ in aqueous solutions

Author

Xiu-Yan Dong, Dong-Cheng Hu, Yan Fan, Jia-Cheng Liu, Chang-Dai Si, Xiao-Qiang Yao, Ya-Jun Wu, and Yun-Xia Yang

Subjects
chemistry.chemical_classification, Aqueous solution, 010405 organic chemistry, Chemistry, Stereochemistry, Hydrogen bond, Supramolecular chemistry, Polymer, 010402 general chemistry, Condensed Matter Physics, 01 natural sciences, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, Inorganic Chemistry, Phthalic acid, chemistry.chemical_compound, Crystallography, Materials Chemistry, Ceramics and Composites, Cluster (physics), Imidazole, Physical and Theoretical Chemistry, Luminescence
Abstract

A novel series of Zn/Cd coordination polymers based on H3L, namely, [Zn2(HL)2(bipy)2(H2O)6]n (1), [Zn(HL)(phen)]n (2), [Cd3L2(bbi)3]n (3), [Zn3L2(bbi)3]n (4) [(H3L =4-[(1-carboxynaphthalen-2-yl)oxy]phthalic acid, bipy =4,4′-bipyridine, phen =1,10-phenanthroline, bbi =1,1′-(1,4-butanediyl)bis(imidazole] have been successfully synthesized by solvothermal reaction. Compound 1 possesses two diverse 1D chains constructed by different bipy coligands, which were further connected to form a 3D supramolecular architecture by hydrogen bonding interactions. Compound 2 possesses a complicated 1D chain based on secondary building unit (SBU) with binuclear Zn cluster. Compounds 3 and 4 exhibit similar 2D→3D framework, which can be rationalized as (3,4,4)-connected 3D net with a Schlafli symbol of (63.8.102)2(63)2(64.8.10). In particular, compound 3 exhibited a high sensitivity for Cr3+ in aqueous solutions, which suggest that compound 3 is a promising luminescent probe for selectively sensing Cr3+.

Published
2016
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115. Effect of N-Donor Ligands and Metal Ions on the Coordination Polymers Based on a Semirigid Carboxylic Acid Ligand: Structures Analysis, Magnetic Properties, and Photoluminescence

Author

Xiao-Qiang Yao, Jia-Cheng Liu, Chang-Dai Si, Dong-Cheng Hu, Yan Fan, Yun-Xia Yang, and Xiu-Yan Dong

Subjects
chemistry.chemical_classification, Photoluminescence, 010405 organic chemistry, Ligand, Stereochemistry, Hydrogen bond, Carboxylic acid, Metal ions in aqueous solution, Supramolecular chemistry, General Chemistry, 010402 general chemistry, Condensed Matter Physics, 01 natural sciences, 0104 chemical sciences, Crystallography, Phthalic acid, chemistry.chemical_compound, chemistry, General Materials Science, Topology (chemistry)
Abstract

Seven novel coordination polymers derived from the 3-(3′,5′-dicarboxylphenoxy)phthalic acid (H4L) ligand have been constructed under similar synthesized conditions with Co(II), Zn(II), Cd(II) ions in the presence of N-donor ancillary ligands, namely, [Co2(HL)(4,4′-bipy)(μ3-OH)(μ2-H2O)]n (1), [Co2(L)(dpa)2]n (2), {[Co2(L)(4,4′-bibp)(H2O)4]·H2O}n (3), [Co2(L)(dib)2]n (4), [Co2(L)(bix)1.5]n (5), [Zn2(L)(bix)1.5]n (6), and {[Cd2(L)(bix)0.5(H2O)]·2H2O}n (7). The various topologies of the structures have been generated by adjusting the N-donor ligands and the central metal ions. Complex 1 displays a tfz-d topology with (3,8)-connected structure, and the Schlafli symbol is (43)2(46.618.84). Complex 2 shows a 4-connected structure of sod topology with a Schlafli symbol of (42.64), forming a three-dimensional (3D) 2-fold interpenetrating structure. Complex 3 presents a novel (3,3,4)-connected topology with a Schlafli symbol of (63.83)(63)(83) and shows 2D → 3D supramolecular structure through C–H···O hydrogen bond...

Published
2016
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116. Perovskite solar cells employing copper (Ⅰ/II) porphyrin hole-transport material with enhanced performance

Author

Xudong Lv, Shi-Jia Long, and Chang-Dai Si

Subjects
Fabrication, Materials science, business.industry, chemistry.chemical_element, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, Copper, Porphyrin, Redox, 0104 chemical sciences, Inorganic Chemistry, chemistry.chemical_compound, chemistry, Materials Chemistry, Optoelectronics, Thermal stability, Physical and Theoretical Chemistry, 0210 nano-technology, business, Perovskite (structure)
Abstract

Perovskite solar cells, the commonly used hole transport materials (HTMs), Spiro-OMeTAD, are considered to be responsible for its long-term instability at high temperature. Herein, it is imperative to design an efficient and stable acylhydrazone-based copper porphyrins CuP as HTMs. Benefiting from the redox shuttle process of copper ion(I/II) in CuP, the performances of corresponding PSCs devices based on CuP as HTM were almost consistent with those of PSCs with Spiro-OMeTAD as HTM. More importantly, compared with the common Spiro-OMeTAD-based devices, the thermal stability of CuP-based devices was improved greatly. This work indicates that the employment of CuP as HTM can achieve the fabrication of efficient and stable PSCs.

Published
2020
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122. Zinc(II), nickel(II) and cadmium(II) coordination polymers based on the ligand oxamide N,N-bis(4-phthalic acid): synthesis, structural characterization and magnetic properties

Author

Wen-Ting Deng, Ibrahim Omar Osman, Ya-Jun Wu, Chen Hou, Chang-Dai Si, Yan Fan, Jia-Cheng Liu, and Dong-Chen Hu

Subjects
010405 organic chemistry, Oxamide, Coordination polymer, Hydrogen bond, Inorganic chemistry, Metals and Alloys, Supramolecular chemistry, chemistry.chemical_element, 010402 general chemistry, 01 natural sciences, 0104 chemical sciences, Inorganic Chemistry, chemistry.chemical_compound, Phthalic acid, Nickel, Crystallography, chemistry, Materials Chemistry, Molecule, Organometallic chemistry
Abstract

Three coordination polymers based on the new ligand oxamide N,N-bis(4-phthalic acid), namely [Zn(L)0.5-(2,2′-bpy)] n (1), [Ni2(2,2′-bpy)4(µ 2-Ox)]L·3H2O (2) and [Cd(L)(1,10-phen)] (3) [L = oxamide N,N-bis(4-phthalic acid)], (2,2′-bpy = 2,2′-bipyridine), (1,10-phen = 1,10-phenanthroline), have been solvothermally synthesized and structurally characterized by single-crystal X-ray diffraction: compound 1 is one-dimensional ladder-like coordination polymer, compound 2 exhibits a three-dimensional structure resulting in extensive hydrogen bonds built with the help of lattice water molecules, compound 3 also exhibits a three-dimensional supramolecular structure. All compounds were also characterized by elemental analysis, IR spectra and thermogravimetric analysis; furthermore, the magnetic measurements for 2 reveal antiferromagnetic coupling between the nickel(II) ions.

Published
2015
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123. Three-Dimensional Supramolecular Architectures with CoII Ions Assembled from Hydrogen Bonding and π···π Stacking Interactions: Crystal Structures and Antiferromagnetic Properties

Author

Jia-Cheng Liu, Dong-Cheng Hu, Yun-Xia Yang, Chang-Dai Si, Yan Fan, Xiao-Qiang Yao, and Xiu-Yan Dong

Subjects
Hydrogen bond, Supramolecular chemistry, Stacking, General Chemistry, Crystal structure, Condensed Matter Physics, chemistry.chemical_compound, Phthalic acid, Crystallography, chemistry, Cluster (physics), Antiferromagnetism, General Materials Science, Benzene
Abstract

Five CoII complexes based on H3L1 and H3L2, namely, [Co3(L1)2(4,4′-bipy)(H2O)3]n (1), [Co3(L1)2(4,4′-bipy)3(H2O)4]n (2), {[Co2(L1)(4,4′-bibp)(μ3-OH)(H2O)]·H2O}n (3), {[Co3(L2)2(dib)3]·2H2O}n (4), and [Co3(L2)2(4,4′-bibp)3]n (5) [(H3L1 = 4-[(3-carboxynaphthalen-2-yl)oxy]phthalic acid, H3L2 = 3-[(3-carboxynaphthalen-2-yl)oxy]phthalic acid, 4,4′-bipy = 4,4′-bipyridine, dib = 1,4-di(1H-imidazol-1-yl) benzene, and 4,4′-bibp = 4,4′-bis(imidazol)-biphenyl] have been synthesized by solvothermal reactions. Complex 1 displays a (4,4,6)-connected topology with a Schlafli symbol of (4.104.12)(411.64)(45.6)2, and the two-dimensional (2D) structure was assembled to form a three-dimensional (3D) framework by C–H···π interactions of internaphthalene rings, showing the dominant antiferromagnetic exchanges in a triangular {Co3} cluster. Complex 2 presents a 2D (3,4,4)-connected network with a Schlafli symbol of (5.62)2(52.63.7)2(54.82) and weakly antiferromagnetic behavior. Complex 3 features a novel (4,4,8)-connected topo...

Published
2015
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124. Three complexes of manganese(II) based on a new semirigid tetracarboxylate and N-containing ligands: Synthesis, crystal structures and magnetic properties

Author

Yun-Xia Yang, Jia-Cheng Liu, Xiao-Qiang Yao, Dong-Chen Hu, Chen Hou, Chang-Dai Si, and Yan Fan

Subjects
Thermogravimetric analysis, Hydrogen bond, Supramolecular chemistry, chemistry.chemical_element, Manganese, Crystal structure, Inorganic Chemistry, chemistry.chemical_compound, Crystallography, chemistry, Materials Chemistry, Physical and Theoretical Chemistry, Benzene, Single crystal, Powder diffraction
Abstract

Three manganese(II) coordination polymers, namely [Mn(L)0.5(bipy)0.5(H2O)]n (1), [Mn2(L)(1,4-bimb)(H2O)2]n (2) and [Mn(L)0.5(4,4′-bibp)0.5(H2O)2]n (3), where H4L = 5,5′-([1,1′-biphenyl]-4,4′-diylbis(oxy))diisophthalic acid, bipy = 4,4′-bipyridine, 1,4-bimb = 1,4-bis(imidazol-1-ylmethyl) benzene and 4,4′-bibp = 4,4′-di(1H-imidazol-1-yl)-1,1′-biphenyl, have been successfully constructed using the hydrothermal method. To understand these compounds completely, single crystal X-ray diffraction, IR, powder X-ray diffraction (PXRD) and thermogravimetric studies have been carried out. Complexes 1 and 2 possess three-dimensional (3D) pillared-layer frameworks, and 2-fold and 3-fold interpenetration take place in 1 and 2, respectively. In complex 3, 2D layers are linked by hydrogen bonds, resulting in a 3D supramolecular architecture. Meanwhile, the magnetic behaviors of complexes 1 and 2 have been investigated and weak antiferromagnetic interactions between two Mn(II) centres within the polymers have been revealed.

Published
2015
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125. Seven Coordination Polymers Derived from Semirigid Tetracarboxylic Acids and N-Donor Ligands: Topological Structures, Unusual Magnetic Properties, and Photoluminescences

Author

Yun-Xia Yang, Chang-Dai Si, Xiao-Qiang Yao, Dong-Cheng Hu, Jia-Cheng Liu, Yu Wu, and Yan Fan

Subjects
chemistry.chemical_classification, Phthalic acid, chemistry.chemical_compound, Secondary building unit, Crystallography, chemistry, Stereochemistry, General Materials Science, General Chemistry, Polymer, Seven coordination, Condensed Matter Physics
Abstract

Seven new complexes based on H4L1 and H4L2, namely, [Cd2(L1)(H2O)5]n (1), {[Cu5(L1)2(μ3-OH)2(H2O)4]·2H2O}n (2), {[Cd2(L1)(4,4′-bipy)2(H2O)3]·2H2O}n (3), [Zn2(L1)(4,4′-bipy)(H2O)3]n (4), {[Ni7(L1)4(4,4′-bipy)12(H2O)12]·2H2O}n (5), {[Ni1.5(L2)(4,4′-bipy)1.5(H2O)4]·H2O}n (6), and {[Ni9(L2)6(dib)12(H2O)18]·18H2O}n (7) [(H4L1 = 4-(3′,5′-dicarboxylphenoxy)phthalic acid, H4L2 = 3-(3′,5′-dicarboxylphenoxy)phthalic acid, 4,4′-bipy = 4,4′-bipyridine, dib = 1,4-di(1H-imidazol-1-yl)benzene], have been synthesized by solvothermal reactions. Complex 1 possesses a 4-connected chiral three-dimensional (3D) structure with a Schlafli symbol of (4.63.82). Complex 2 presents a (4,6)-connected porous architecture based on a chair-shaped [Cu4(μ3-OH)2]6+ secondary building unit (SBU) with a Schlafli symbol of (43.63)2(46.66.83), revealing unusual ferromagnetic behavior. Complex 3 exhibits a 2D → 3D framework through H-bonding interactions, which is a (3,3,4)-connected 2-fold interpenetrated structure with a Schlafli symbol of (...

Published
2015
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126. Touch technology based on mixed color space and codebook background difference

Author

林孜铠 Lin Zi-kai, 苏剑邦 Su Jian-bang, 梁浩文 Liang Hao-wen, 林岱昆 Lin Dai-kun, 戴思璋 Dai Si-zhang, 王嘉辉 Wang Jia-hui, 朱洁霞 Zhu Jie-xia, 陈海域 Chen Hai-yu, and 周建英 Zhou Jian-ying

Subjects
Computer science, business.industry, Computer graphics (images), Signal Processing, Codebook, Computer vision, Artificial intelligence, Color space, business, Instrumentation, Electronic, Optical and Magnetic Materials
Published
2015
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127. Morphological variation of Chrysanthemum lavandulifolium induced by 5-azaC treatment

Author

Luo Hong, Kang Dong-ru, Dai Si-lan, Zhang Fan, and Gao Kang

Subjects
0106 biological sciences, 0301 basic medicine, Regeneration (biology), fungi, food and beverages, DNA Methyltransferase Inhibitor, Asexual reproduction, Horticulture, Biology, 01 natural sciences, Hypocotyl, Plantlet, 03 medical and health sciences, 030104 developmental biology, Flower induction, DNA methylation, 010606 plant biology & botany, Explant culture
Abstract

To explore the possible role of DNA methylation in plant development. Different concentrations of the DNA methyltransferase inhibitor 5-azacytidine were used on different components of Chrysanthemum lavandulifolium (stem and hypocotyl). The effects of 5-azaC on plantlet growth and flower development were investigated. Results showed that low concentrations of 5-azaC (20 μmol L−1–100 μmol L−1) inhibited plantlet growth (from the stem segments), and high concentrations of 5-azaC (150 μmol L−1–200 μmol L−1) promoted plantlet growth. This inhibition is similar in the regeneration of hypocotyls, except that the concentration range of 5-azaC is different. Low concentration of 5-azaC (10 μmol L−1–20 μmol L−1) inhibit generation or growth of callus-generated plantlets, high concentration of 5-azaC (150 μmol L−1–200 μmol L−1) promoted the growth of hypocotyl regeneration seedlings. In thus, the inhibitory effect of plantlet was similar in hypocotyl regeneration. Therefore, different explants have different sensitivities to methyltransferase inhibitor (5-azaC) treatment. Progeny plants treated with stems of C. lavandulifolium under the short days condition, the control plants (about 15 leaves) did not bloom, plants undergoing high concentration treatments flowered in some strains (flowering rate 80% in treatment 6), and the pitch of all unflowering plants was dwarfed. It seems that 5-azaC can break the C. lavandulifolium adolescent period limit, potentially by inducing the photoperiodic in advance or through another mechanism, after 5-azaC processing may alters key gene methylation, resulting in flowering. In the hypocotyl-treated progeny after multiple passages, all treatments had flowering and non-flowering lines under short-day conditions, and the flowering rate was between 39.29% and 78.43%. We also obtained a continuous flowering line was obtained (2-8-2), and this trait exhibited stable by asexual reproduction. In addition, abundant variation in leaf morphology, flower shape and stem color variation were observed in hypocotyl regeneration plants. All the evidence suggests that the methyltransferase inhibitor (5-azaC) may affect the DNA methylation level of many key genes regulating plant growth and development. This study provides phenotypic data and materials for further exploring the role of DNA methylation in the growth and flower induction of C. lavandulifolium.

Published
2019
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134. Complexes built with a scorpion-type tripodal ligand and metal ions: syntheses, structural features, electrochemical properties and magnetic properties

Author

Chang-Dai Si, Chao-Hu Xiao, Dong-Cheng Hu, Xue-Yan Song, Yun-Xia Yang, and Jia-Cheng Liu

Subjects
Coordination sphere, Metal ions in aqueous solution, Inorganic chemistry, Metals and Alloys, Atmospheric temperature range, Electrochemistry, Magnetic susceptibility, Inorganic Chemistry, chemistry.chemical_compound, Crystallography, chemistry, Octahedron, Tripodal ligand, Materials Chemistry, Organometallic chemistry
Abstract

The complexes [Fe(bpz*mpy)2](ClO4)2 (1a), [Cu(bpz*mpy)2](ClO4)2 (1b) and [Ag(bpz*mpy)(Ph3P)](ClO4) · H2O (2) (bpz*mpy = pyridin-2-yl-bis(3,5-dimethylpyrazol-1-yl)methane) have been synthesized and characterized by physicochemical and spectroscopic methods. X-ray crystallographic analysis reveals that the central FeII and CuII ions in complexes 1a and 1b are located on a twofold rotation axis and have a distorted octahedral coordination sphere, while the AgI center in complex 2 is tetrahedrally coordinated. The electrochemical properties of complex 1b have been investigated. Furthermore, a variable temperature magnetic susceptibility study of complex 1a has also been performed over the measured temperature range 2–300 K.

Published
2013
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135. Global patient outcomes after elective surgery: prospective cohort study in 27 low-, middle- and high-income countries The International Surgical Outcomes Study group

Author

Ahmad, T, Bouwman, RA, Grigoras, I, Aldecoa, C, Hofer, C, Hoeft, A, Holt, P, Fleisher, LA, Buhre, W, Pearse, RM, Ferguson, Marissa, MacMahon, Michael, Shulman, Mark, Cherian, Ritchie, Currow, Helen, Kanathiban, Kathirgamanathan, Gillespie, David, Pathmanathan, Edward, Phillips, Katherine, Reynolds, Jenifer, Rowley, Joanne, Douglas, Jeanene, Kerridge, Ross, Garg, Sameer, Bennett, Michael, Jain, Megha, Alcock, David, Terblanche, Nico, Cotter, Rochelle, Leslie, Kate, Stewart, Marcelle, Zingerle, Nicolette, Clyde, Antony, Hambidge, Oliver, Rehak, Adam, Cotterell, Sharon, Huynh, Wilson Binh Quan, McCulloch, Timothy, Ben-Menachem, Erez, Egan, Thomas, Cope, Jennifer, Halliwell, Richard, Fellinger, Paul, Haisjackl, Markus, Haselberger, Simone, Holaubek, Caroline, Lichtenegger, Paul, Scherz, Florian, Schmid, Werner, Hoffer, Franz, Cakova, Veronika, Eichwalder, Andreas, Fischbach, Norbert, Klug, Reinhold, Schneider, Elisabeth, Vesely, Martin, Wickenhauser, Reinhart, Grubmueller, Karl Gernot, Leitgeb, Marion, Lang, Friedrich, Toro, Nancy, Bauer, Marlene, Laengle, Friedrich, Haberl, Claudia, Mayrhofer, Thomas, Trybus, Christoph, Buerkle, Christian, Forstner, Karin, Germann, Reinhard, Rinoesl, Harald, Schindler, Elke, Trampitsch, Ernst, Bogner, Gerhard, Dankl, Daniel, Duenser, Martin, Fritsch, Gerhard, Gradwohl-Matis, Ilse, Hartmann, Andreas, Hoelzenbein, Thomas, Jaeger, Tarkan, Landauer, Franz, Lindl, Gregor, Lux, Michael, Steindl, Johannes, Stundner, Ottokar, Szabo, Christian, Bidgoli, Jawad, Verdoodt, Hans, Forget, Patrice, Kahn, David, Lois, Fernande, Momeni, Mona, Pregardien, Caroline, Pospiech, Audrey, Steyaert, Arnaud, Veevaete, Laurent, De Kegel, Dirk, De Jongh, Karen, Foubert, Luc, Smitz, Carine, Vercauteren, Marcel, Poelaert, Jan, Van Mossevelde, Veerle, Abeloos, Jacques, Bouchez, Stefaan, Coppens, Marc, De Baerdemaeker, Luc, Deblaere, Isabel, De Bruyne, Ann, De Hert, Stefan, Fonck, Kristine, Heyse, Bjorn, Jacobs, Tom, Lapage, Koen, Moerman, Anneliese, Neckebroek, Martine, Parashchanka, Aliaksandra, Roels, Nathalie, Van Den Eynde, Nancy, Vandenheuvel, Michaël, van Limmen, Jurgen, Vanluchene, Ann, Vanpeteghem, Caroline, Wouters, Patrick, WYFFELS, PIET, Huygens, Christel, Vandenbempt, Punitha, Van de Velde, Marc, Dylst, Dimitri, Janssen, Bruno, Schreurs, Evelien, Aleixo, Fabia Berganton, Candido, Keulle, Batista, Hugo Dias, Guimaraes, Mario, Guizeline, Jaqueline, Hoffmann, Joao, Lobo, Suzana, Marques Lobo, Francisco Ricardo, Nascimento, Vinicius, Nishiyama, Katia, Pazetto, Lucas, Souza, Daniela, Rodrigues, Rodrigo Souza, Vilela dos Santos, Ana Maria, Jardim, Jaquelline, Sa Malbouisson, Luiz Marcelo, Silva, Joao, do Nascimento Junior, Paulo, Baio, Thalissa Herminia, Pereira de Castro, Gabriel Isaac, Watanabe Oliveira, Henri Roger, Amendola, Cristina Prata, Cardoso, Gutemberg, Ortega, Daniela, Brotto, Ana Flavia, De Oliveira, Mirella Cristine, Rea-Neto, Alvaro, Dias, Fernando, Travi, Maria Eduarda, Zerman, Luiza, Azambuja, Pedro, Knibel, Marcos 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Shelley, Campbell, Tara, James, Sarah, King, Chris, de Araujo, Daniela Marques, Martin, Daniel, Morkane, Clare, Neely, Julia, Rajendram, Rajkumar, Burton, Megan, James, Kathryn, Keevil, Edward, Minik, Orsolya, Morgan, Jenna, Musgrave, Anna, Rajanna, Harish, Roberts, Tracey, Szakmany, Tamas, Adamson, Michael, Jumbe, Sandra, Kendall, Jennie, Muthuswamy, Mohan Babu, Anderson, Charlotte, Cruikshanks, Andrew, Wrench, Ian, Zeidan, Lisa, Ardern, Diane, Harris, Benjamin, Hellstrom, Johanna, Martin, Jane, Thomas, Richard, Varsani, Nimu, Brown, Caroline Wrey, Docherty, Philip, Gillies, Michael, McGregor, Euan, Usher, Helen, Craig, Jayne, Smith, Andrew, Ahmad, Tahania, Bodger, Phoebe, Creary, Thais, Everingham, Kirsty, Fowler, Alexander, Hewson, Russ, Ijuo, Eke, Januszewska, Marta, Jones, Timothy, Kantsedikas, Ilya, Lahiri, Sumitra, McLean, Aaron Lawson, Niebrzegowska, Edyta, Phull, Mandeep, Wang, Difei, Wickboldt, Nadine, Baldwin, Jacqueline, Doyle, Donna, Mcmullan, Sean, Oladapo, Michelle, Owen, Thomas, Williams, Alexandra, Daniel, Hull, Gregory, Peter, Husain, Tauqeer, Kirk-Bayley, Justin, Mathers, Edward, Montague, Laura, Harper, Mark, White, Stuart, Jack, James, Ridley, Carrie, Avis, Joanne, Cook, Tim, Dali-Kemmery, Lola, Kerslake, Ian, Lambourne, Victoria, Pearson, Annabel, Boyd, Christine, Callaghan, Mark, Lawson, Cathy, McCrossan, Roopa, Nesbitt, Vanessa, O'connor, Laura, Scott, Julia, Sinclair, Rhona, Farid, Nahla, Morgese, Ciro, Bhatia, Kailash, Karmarkar, Swati, Ahmed, Jamil, Branagan, Graham, Hutton, Monica, Swain, Andrew, Brookes, Jamie, Cornell, Jonathan, Dolan, Rachael, Hulme, Jonathan, van Vuuren, Amanda Jansen, Jowitt, Tom, Kalashetty, Gunasheela, Lloyd, Fran, Patel, Kiran, Sherwood, Nicholas, Brown, Lynne, Chandler, Ben, Deighton, Kerry, Emma, Temlett, Haunch, Kirsty, Cheeseman, Michelle, Dent, Kathy, Garg, Sanjeev, Gray, Carol, Hood, Marion, Jones, Dawn, Juj, Joanne, Rao, Roshan, Walker, Tara, Al Anizi, Mashel, Cheah, Clarissa, Cheing, Yushio, Coutinho, Francisco, Gondo, Prisca, Hadebe, Bernard, Hove, Mazvangu Onie, khader, Ahamed, Krishnachetty, Bobby, Rhodes, Karen, Sokhi, Jagdish, Baker, Katie-Anne, Bertram, Wendy, Looseley, Alex, Mouton, Ronelle, Hanna, George, Arnold, Glenn, Arya, Shobhit, Balfoussia, Danai, Baxter, Linden, Harris, James, Jones, Craig, Knaggs, Alison, Markar, Sheraz, Perera, Anisha, Scott, Alasdair, Shida, Asako, Sirha, Ravneet, Wright, Sally, Frost, Victoria, Gray, Catherine, Andrews, Emma, Arrandale, Lindsay, Barrett, Stephen, Cifra, Elna, Cooper, Mariese, Dragnea, Dragos, Elna, Cifra, Maclean, Jennifer, Meier, Sonja, Milliken, Donald, Munns, Christopher, Ratanshi, Nadir, Ramessur, Suneil, Salvana, Abegail, Watson, Anthony, Ali, Hani, Campbell, Gill, Critchley, Rebecca, Endersby, Simon, Hicks, Catherine, Liddle, Alison, Pass, Marc, Ritchie, Charlotte, Thomas, Charlotte, Too, Lingxi, Welsh, Sarah, Gill, Talvinder, Johnson, Joanne, Reed, Joanne, Davis, Edward, Papadopoullos, Sam, Attwood, Clare, Biffen, Andrew, Boulton, Kerenza, Gray, Sophie, Hay, David, Mills, Sarah, Montgomery, Jane, Riddell, Rory, Simpson, James, Bhardwaj, Neeraj, Paul, Elaine, Uwubamwen, Nosakhare, Alexander, Maini, Arrich, James, Arumugam, Swarna, Blackwood, Douglas, Boggiano, Victoria, Brown, Robyn, Chan, Yik Lam, Chatterjee, Devnandan, Chhabra, Ashok, Christian, Rachel, Costelloe, Hannah, Matthewman, Madeline Coxwell, Dalton, Emma, Darko, Julia, Davari, Maria, Dave, Tejal, Deacon, Matthew, Deepak, Shantal, Edmond, Holly, Ellis, Jessica, El-Sayed, Ahmed, Eneje, Philip, English, Rose, Ewe, Renee, Foers, William, Franklin, John, Gallego, Laura, Garrett, Emily, Goldberg, Olivia, Goss, Harry, Greaves, Rosanna, Harris, Rudy, Hennings, Charles, Jones, Eleanor, Kamali, Nelson, Kokkinos, Naomi, Lewis, Carys, Lignos, Leda, Malgapo, Evaleen Victoria, Malik, Rizwana, Milne, Andrew, Mulligan, John-Patrick, Nicklin, Philippa, Palipane, Natasha, Parsons, Thomas, Piper, Rebecca, Prakash, Rohan, Ramesh, Byron, Rasip, Sarah, Reading, Jacob, Rela, Mariam, Reyes, Anna, Stephens, Robert, Rooms, Martin, Shah, Karishma, Simons, Henry, Solanki, Shalil, Spowart, Emma, Stevens, Amy, Thomas, Christopher, Waggett, Helena, Yassaee, Arrash, Kennedy, Anthony, Scott, Sara, Somanath, Sameer, Berg, Andrew, Hernandez, Miguel, Nanda, Rajesh, Tank, Ghanshyambhai, Wilson, Natalie, Wilson, Debbie, Al-Soudaine, Yassr, Baldwin, Matthew, Cornish, Julie, Davies, Zoe, Davies, Leigh, Edwards, Marc, Frewer, Natasha, Gallard, Sian, Glasbey, James, Harries, Rhiannon, Hopkins, Luke, Kim, Taeyang, Koompirochana, Vilavan, Lawson, Simon, Lewis, Megan, Makzal, Zaid, Scourfield, Sarah, Ahmad, Yousra, Bates, Sarah, Blackwell, Clare, Bryant, Helen, Collins, Hannah, Coulter, Suzanne, Cruickshank, Ross, Daniel, Sonya, Daubeny, Thomas, Edwards, Mark, Golder, Kim, Hawkins, Lesley, Helen, Bryant, Hinxman, Honor, Levett, Denny, Salmon, Karen, Seaward, Leanne, Skinner, Ben, Tyrell, Bryony, Wadams, Beverley, Walsgrove, Joseph, Dickson, Jane, Constantin, Kathryn, Karen, Markwell, O'Brien, Peter, O'Donohoe, Lynn, Payne, Hannah, Sundayi, Saul, Walker, Elaine, Brooke, Jenny, Cardy, Jon, Humphreys, Sally, Kessack, Laura, Kubitzek, Christiane, Kumar, Suhas, Cotterill, Donna, Hodzovic, Emil, Hosdurga, Gurunath, Miles, Edward, Saunders, Glenn, Campbell, Marta, Chan, Peter, Jemmett, Kim, Raj, Ashok, Naik, Aditi, Oshowo, Ayo, Ramamoorthy, Rajarajan, Shah, Nimesh, Sylvan, Axel, Blyth, Katharine, Burtenshaw, Andrew, Freeman, David, Johnson, Emily, Lo, Philip, Martin, Terry, Plunkett, Emma, Wollaston, Julie, Allison, Joanna, Carroll, Christine, Craw, Nicholas, Craw, Sarah, Pitt-Kerby, Tressy, Rowland-Axe, Rebecca, Spurdle, Katie, McDonald, Andrew, Simon, Davies, Sinha, Vivek, Smith, Thomas, Banner-Goodspeed, Valerie, Boone, Myles, Campbell, Kathleen, Lu, Fengxin, Scannell, Joseph, Sobol, Julia, Balajonda, Naraida, Clemmons, Karen, Conde, Carlos, Elgasim, Magdi, Funk, Bonita, Hall, Roger, Hopkins, Thomas, Olaleye, Omowunmi, Omer, Omer, Pender, Michelle, Porto, Angelo, Stevens, Alice, Waweru, Peter, Yeh, Erlinda, Bodansky, Daniella, Evans, Adam, Kleopoulos, Steven, Maril, Robert, Mathney, Edward, Sanchez, Angela, Tinuoye, Elizabeth, Bateman, Brian, Eng, Kristen, Jiang, Ning, Ladha, Karim, Needleman, Joseph, Chen, Lee-lynn, Lane, Rondall, Robinowitz, David, Ghushe, Neil, Irshad, Mariam, O'Connor, John, Patel, Samir, Takemoto, Steven, Wallace, Art, Mazzeffi, Michael, Rock, Peter, Wallace, Karin, Zhu, Xiaomao, Chua, Pandora, Fleisher, Lee, Mattera, Matthew, Sharar, Rebecca, Thilen, Stephan, Treggiari, Miriam, Morgan, Angela, Sofjan, Iwan, Subramaniam, Kathirvel, Avidan, Michael, Maybrier, Hannah, Muench, Maxwell, and Wildes, Troy

Subjects
surgery, postoperative care/methods, cohort studies, operative/mortality, data, postoperative care/statistics and numerical, Medicine and Health Sciences, critical care/utilisation, surgical procedures
Abstract

Background: As global initiatives increase patient access to surgical treatments, there remains a need to understand the adverse effects of surgery and define appropriate levels of perioperative care. Methods: We designed a prospective international 7-day cohort study of outcomes following elective adult inpatient surgery in 27 countries. The primary outcome was in-hospital complications. Secondary outcomes were death following a complication (failure to rescue) and death in hospital. Process measures were admission to critical care immediately after surgery or to treat a complication and duration of hospital stay. A single definition of critical care was used for all countries. Results: A total of 474 hospitals in 19 high-, 7 middle- and 1 low-income country were included in the primary analysis. Data included 44 814 patients with amedian hospital stay of 4 (range 2-7) days. A total of 7508 patients (16.8%) developed one or more postoperative complication and 207 died (0.5%). The overall mortality among patients who developed complications was 2.8%. Mortality following complications ranged from 2.4% for pulmonary embolism to 43.9% for cardiac arrest. A total of 4360 (9.7%) patients were admitted to a critical care unit as routine immediately after surgery, of whom 2198 (50.4%) developed a complication, with 105 (2.4%) deaths. A total of 1233 patients (16.4%) were admitted to a critical care unit to treat complications, with 119 (9.7%) deaths. Despite lower baseline risk, outcomes were similar in low- and middle-income compared with high-income countries. Conclusions: Poor patient outcomes are common after inpatient surgery. Global initiatives to increase access to surgical treatments should also address the need for safe perioperative care.

Published
2016

136. Microemulsion synthesis of ZnMn2O4/Mn3O4sub-microrods for Li-ion batteries and their conversion reaction mechanism

Author

FENG, Ting-ting, YANG, Jian, DAI, Si-yi, WANG, Jun-chao, and WU, Meng-qiang

Abstract

The hierarchical ZnMn2O4/Mn3O4composite sub-microrods were synthesized via a water-in-oil micro- emulsion method followed by calcination. The ZnMn2O4/Mn3O4electrode displays an intriguing capacity increasing from 440 to 910 mA·h/g at 500 mA/g during 550 consecutive discharge/charge cycles, and delivers an ultrahigh capacity of 1276 mA·h/g at 100 mA/g, which is much greater than the theoretical capacity of either ZnMn2O4or Mn3O4electrode. To investigate the underlying mechanism of this phenomenon, cyclic voltammetry and differential capacity analysis were applied, both of which reveal the emergence and the growth of new reversible redox reactions upon charge/discharge cycling. The new reversible conversions are probably the results of an activation process of the electrode material during the cycling process, leading to the climbing charge storage. However, the capacity exceeding the theoretical value indicates that there are still other factors contributing to the increasing capacity.

Published
2021
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145. Formulation engineering for optimizing ternary electron acceptors exemplified by isomeric PC71BM in planar perovskite solar cells

Author

Dai, Si-Min, primary, Zhang, Xin, additional, Chen, Wei-Yi, additional, Li, Xiang, additional, Tan, Zhan'ao, additional, Li, Cong, additional, Deng, Lin-Long, additional, Zhan, Xin-Xing, additional, Lin, Min-Song, additional, Xing, Zhou, additional, Wen, Tao, additional, Ho, Rong-Ming, additional, Xie, Su-Yuan, additional, Huang, Rong-Bin, additional, and Zheng, Lan-Sun, additional

Published
2016
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147. Flower colour modification of chrysanthemum by suppression of F3'H and overexpression of the exogenous Senecio cruentus F3'5'H gene

Author

Huang He, Hu Ke, Dai Si-lan, Han KeTing, and Xiang Qiaoyan

Subjects
Chrysanthemum, Cyanidin, lcsh:Medicine, Color, Cut flowers, Flowers, Biology, Senecio, Anthocyanins, chemistry.chemical_compound, Gene Expression Regulation, Plant, Botany, Ornamental plant, Cultivar, lcsh:Science, Multidisciplinary, Pigmentation, lcsh:R, biology.organism_classification, Plants, Genetically Modified, chemistry, Anthocyanin, Petal, lcsh:Q, Delphinidin, Research Article
Abstract

Chrysanthemum (Chrysanthemum × morifolium) is one of the most important ornamental plants in the world. They are typically used as cut flowers or potted plants. Chrysanthemum can exhibit red, purple, pink, yellow and white flowers, but lack bright red and blue flowers. In this study, we identified two chrysanthemum cultivars, C × morifolium ‘LPi’ and C × morifolium ‘LPu’, that only accumulate flavonoids in their ligulate flowers. Next, we isolated seven anthocyanin biosynthesis genes, namely CmCHS, CmF3H, CmF3’H, CmDFR, CmANS, CmCHI and Cm3GT in these cultivars. RT-PCR and qRT-PCR analyses showed that CmF3′H was the most important enzyme required for cyanidin biosynthsis. To rebuild the delphinidin pathway, we downregulated CmF3’H using RNAi and overexpressed the Senecio cruentus F3′5′H (PCFH) gene in chrysanthemum. The resultant chrysanthemum demonstrated a significantly increased content of cyanidin and brighter red flower petals but did not accumulate delphinidin. These results indicated that CmF3′H in chrysanthemum is important for anthocyanin accumulation, and Senecio cruentus F3′5′H only exhibited F3′H activity in chrysanthemum but did not rebuild the delphinidin pathway to form blue flower chrysanthemum.

Published
2013

148. Biomarqueurs potentiels de l’hypoxie tissulaire au cours de l’anémie par hémodilution de la chirurgie cardiaque: étude prospective évaluant l’hypoxie tissulaire comme mécanisme de lésion d’organes.

Author

Hare, Gregory M. T., Han, Kaho, Leshchyshyn, Yevheniy, Mistry, Nikhil, Kei, Tiffanie, Dai, Si Yuan, Tsui, Albert K. Y., Pirani, Razak A., Honavar, Jaideep, Patel, Rakesh P., Yagnik, Sanjay, Welker, Shelley L., Tam, Tessa, Romaschin, Alexander, Connelly, Philip W., Beattie, W. Scott, and Mazer, C. David

Abstract

Purpose: Hemodilutional anemia is associated with acute kidney injury (AKI) and mortality in patients undergoing cardiac surgery by mechanisms that may include tissue hypoxia. Our hypothesis was to assess if changes in the potential hypoxic biomarkers, including methemoglobin and erythropoietin, correlated with a decrease in hemoglobin (Hb) concentration following hemodilution on cardiopulmonary bypass (CPB).Methods: Arterial blood samples were taken from patients (n = 64) undergoing heart surgery and CPB at baseline, during CPB, following CPB, and in the intensive care unit (ICU). Potential hypoxic biomarkers were measured, including methemoglobin, plasma Hb, and erythropoietin. Data were analyzed by repeated measures one-way analysis of variance on ranks and linear regression.Results: Hemoglobin levels decreased following CPB and methemoglobin increased in the ICU (P < 0.001 for both). No correlation was observed between the change in Hb and methemoglobin (P = 0.23). By contrast, reduced Hb on CPB correlated with increased lactate, reduced pH, and increased erythropoietin levels following CPB (P ≤ 0.004 for all). Increased plasma Hb (P < 0.001) also correlated with plasma erythropoietin levels (P < 0.001).Conclusion: These data support the hypothesis that erythropoietin rather than methemoglobin is a potential biomarker of anemia-induced tissue hypoxia. The observed relationships between decreased Hb during CPB and the increase in lactate, reduced pH, and increase in erythropoietin levels suggest that early changes in plasma erythropoietin may be a pragmatic early biomarker of anemia-induced renal hypoxia. Further study is required to determine if anemia-induced increases in erythropoietin may predict AKI in patients undergoing cardiac surgery.Trial Registration: www.clinicaltrials.gov (NCT01883713). Registered 21 June 2013. [ABSTRACT FROM AUTHOR]

Published
2018
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150. cDNA-AFLP analysis of salt-inducible genes expression in Chrysanthemum lavandulifolium under salt treatment

Author

Niu Yajing, Cao Hua-wen, Tang Xingjiao, Xia XinLi, Dai Si-lan, Yin WeiLun, and Huang He

Subjects
Chlorophyll, Cell Membrane Permeability, DNA, Complementary, Time Factors, Physiology, Sequence analysis, Chrysanthemum, Plant Science, Biology, Sodium Chloride, Genes, Plant, Homology (biology), chemistry.chemical_compound, Plant Growth Regulators, Gene Expression Regulation, Plant, Complementary DNA, Halophyte, Malondialdehyde, Botany, Amplified Fragment Length Polymorphism Analysis, Abscisic acid, Gene, Peroxidase, Expressed Sequence Tags, Expressed sequence tag, food and beverages, Salt-Tolerant Plants, Salt Tolerance, Sequence Analysis, DNA, Droughts, Cold Temperature, Plant Leaves, Biochemistry, chemistry, Amplified fragment length polymorphism, Agronomy and Crop Science, Stress, Psychological, Abscisic Acid
Abstract

Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino is a halophyte species that belongs to the Asteraceae family, and the genus Chrysanthemum. It is one of the ancestors of C.×morifolium Ramatella. Understanding the tolerance mechanism associated with salt stress in C. lavandulifolium could provide important information for explaining the salt tolerance of higher plants and could also help enhancing breeding programs of cultivated Chrysanthemum. In this study, cDNA amplified fragment length polymorphism (cDNA-AFLP) was used to detect differential gene expression in leaves of C. lavandulifolium in response to NaCl treatment. The determination of membrane permeablility, peroxidase activity (POD), malon-dialdehyde (MDA), as well as proline and leaf chlorophyll contents under different NaCl concentrations showed that a 200 mM NaCl treatment was an optimal condition for the cDNA-AFLP experiment. Using this concentration during different times (0, 3 h, 12 h, 24 h and 48 h), we obtained 1930 cDNA fragments using 64 primers. After sequencing 234 randomly chosen cDNA clones and BLASTx analyzing, we got 129 expressed sequence tags (ESTs) which had no significant homology with other sequences, 85 ESTs were homologous to genes with known functions, whereas the rest of ESTs showed homology to unclassified or putative proteins. 25 ESTs that were similar to known functional genes involved in several abiotic and biotic stresses were confirmed by semi-quantitative RT-PCR and qRT-PCR. The expression patterns of these salt-responsive genes not only responded to salt stress but also to plant hormones, such as abscisic acid (ABA), and to other abiotic stresses such as drought and cold. These results indicate an extensive cross-talk among several stresses. Our results provide interesting information for further understanding the molecular mechanisms of salt tolerance in C. lavandulifolium.

Published
2011

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