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819 results on '"Döring G"'

105. Cleavage of lymphocyte surface antigens CD2, CD4, and CD8 by polymorphonuclear leukocyte elastase and cathepsin G in patients with cystic fibrosis

Author

Döring G, Frank F, CHRISTIAN BOUDIER, Herbert S, Fleischer B, and Bellon G

Subjects
Adult, Cathepsin G, Rosette Formation, Cystic Fibrosis, Pancreatic Elastase, Neutrophils, CD8 Antigens, T-Lymphocytes, Immunology, Serine Endopeptidases, CD2 Antigens, Sputum, Cathepsins, Antigens, CD, CD4 Antigens, Endopeptidases, Pseudomonas aeruginosa, Immunology and Allergy, Cytokines, Humans, Child, Leukocyte Elastase, Bronchoalveolar Lavage Fluid, Lung
Abstract

Polymorphonuclear leukocytes (PMN) accumulating in airways of patients with cystic fibrosis (CF) as a response to chronic endobronchial bacterial lung infection, release lysosomal serine proteinases such as PMN-elastase at concentrations of approximately 0.5 microM to 5 microM into the airway lumen. Immunohistology of CF lung material and fluorescence activated cell sorter analysis of sequential CF bronchoalveolar lavages demonstrated loss of the CD4 and CD8 Ag on CD3+ T lymphocytes in sputum-filled airways. In 10 CF sputum samples 1.0%, 19.1%, and 15.7% of all CD3+ T lymphocytes expressed CD4, CD8, and CD2, respectively. Incubation of CF sputum supernatant fluids with peripheral blood T lymphocytes resulted in total reduction of CD4 and CD8 but not CD2. Addition of alpha 1-proteinase inhibitor abolished surface Ag cleavage completely. Purified PMN-elastase and cathepsin G cleaved CD2, CD4, and CD8 on peripheral blood T lymphocytes at proteinase concentrations of 0.83 to 8.3 microM in a dose-dependent manner. Cleaved CD4 and CD8 were reexpressed on the surface of T lymphocytes after 24 h in the absence of PMN-elastase. Incubation of a CD4+ T cell clone with PMN-elastase lead to a significant reduction of cytotoxicity toward target cells and significantly reduced IL-2 and IL-4 production. The results suggest a temporary functional impairment of T lymphocytes in foci of high inflammation characterized by stimulated PMN, which may lower tissue destruction.

Published
1995

106. Post-translational regulation of alginate export by an oxygen sensor in Pseudomonas aeruginosa

Author

Schmidt, A., primary, Limpert, A.S., additional, Bastian, M., additional, Merighi, M., additional, Aktürk, F., additional, Mailänder, S., additional, Madlung, J., additional, Lapouge, K., additional, Poschgan, C., additional, Simm, R., additional, Acharya, K.R., additional, Römling, U., additional, Ulrich, M., additional, Macek, B., additional, Wiehlmann, L., additional, Pier, G.B., additional, Lory, S., additional, Haas, D., additional, and Döring, G., additional

Published
2010
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112. The respiratory burst of PMNs accelerates anaerobiosis in sputum from CF patients with chronic Pseudomonas aeruginosa lung infection

Author

Kolpen, M., primary, Bjarnsholt, T., additional, Moser, C., additional, Christensen, L.D., additional, Gennip, M.V., additional, Hansen, C.R., additional, Ciofu, O., additional, Mandsberg, L., additional, Kharazmi, A., additional, Döring, G., additional, Givskov, M., additional, Høiby, N., additional, and Jensen, P.Ø., additional

Published
2008
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113. Antianginal and anti-ischemic efficacy of nicorandil in comparison with isosorbide-5-mononitrate and isosorbide dinitrate: results from two multicenter, double-blind, randomized studies with stable coronary heart disease patients

Author

Döring G

Subjects
Adult, Male, Niacinamide, medicine.medical_specialty, Potassium Channels, Vasodilator Agents, Blood Pressure, Coronary Disease, Isosorbide Dinitrate, Drug Administration Schedule, law.invention, Angina, Randomized controlled trial, Double-Blind Method, law, Heart Rate, Internal medicine, Medicine, Humans, Dosing, Nicorandil, Exercise, Aged, Pharmacology, business.industry, Middle Aged, medicine.disease, Crossover study, Clinical trial, Regimen, Anesthesia, cardiovascular system, Cardiology, Female, Isosorbide dinitrate, Cardiology and Cardiovascular Medicine, business, medicine.drug
Abstract

The purpose of the two double-blind studies summarized in this article was to compare the antianginal and anti-ischemic effects of nicorandil with those of two different nitrate preparations. A total of 129 patients with stable New York Heart Association functional class II or III coronary heart disease were enrolled in the studies. Ninety-five patients received nicorandil, 34 received isosorbide dinitrate (ISDN), and 63 received isosorbide-5-mononitrate (MN). In study 1, nicorandil was compared with MN in a crossover design with 54 protocols eligible for efficacy assessment of MN and 52 eligible for nicorandil, respectively. Twenty milligrams of nicorandil and 20 mg MN administered b.i.d. for 4 weeks were equally effective in the treatment of stress-induced angina. Both drugs prolonged bicycle exercise tolerance and reduced weekly anginal attack rates. In study 2, nicorandil and ISDN were administered to two parallel groups of patients at a dose of 10 mg t.i.d. for 2 weeks and then 20 mg t.i.d. for 4 weeks. Under the assumption that the repetitive administration of nitrates with short dosing intervals might induce the development of tolerance to the nitrate mechanism of action, the t.i.d.-dosing regimen had been chosen in this study. Thirty-two protocols from those receiving nicorandil and 34 protocols from those receiving ISDN were eligible for efficacy assessment. Both drugs increased exercise capacity and reduced ST-segment depression at identical work loads with no significant difference between groups (p > 0.05). For both drugs, the higher doses were more effective than the lower doses. tolerance to the nitrate mechanism of action did not develop with either drug.(ABSTRACT TRUNCATED AT 250 WORDS)

Published
1992

117. 83 Emergence of an epidemic clone of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) Panton-Valentine leukocidin (PVL) negative in Cystic Fibrosis patients

Author

Cocchi, P., primary, Cariani, L., additional, Favari, F., additional, DelPezzo, M., additional, Lambiase, A., additional, Fiscarelli, E., additional, Gioffrè, F.V., additional, d'Aprile, A., additional, Manso, E., additional, Döring, G., additional, Taccetti, G., additional, and Campana, S., additional

Published
2007
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123. Typing of polyagglutinable Pseudomonas aeruginosa isolates from cystic fibrosis patients

Author

Ojeniyi B, Christiane Wolz, Döring G, Js, Lam, Vt, Rosdahl, and Høiby N

Subjects
DNA, Bacterial, Pyocins, Cystic Fibrosis, Agglutination Tests, Immune Sera, Pseudomonas aeruginosa, Humans, Nucleic Acid Hybridization, Serotyping, DNA Probes, Bacteriophage Typing
Abstract

The study assesses the reproducibility, typability and discriminatory power of several typing methods for Pseudomonas aeruginosa isolated from cystic fibrosis patients. 178 polyagglutinable Pseudomonas aeruginosa isolates from cystic fibrosis patients were serotyped using polyclonal sera and monoclonal antibodies, phage typed, pyocin typed and reverse phage typed. 31 of these polyagglutinable isolates, six monoagglutinable isolates and three nontypable isolates were also typed by means of hybridization using a DNA probe. In a comparison of the methods used, on polyagglutinable isolates only, typability was 0% with polyclonal sera, 90% with monoclonal sera, 94% with phage typing, 85% with pyocin typing, 36% with reverse phage typing and 100% with DNA-prope typing. Using monoclonal antibodies, the reproducibility was 75%, while that of phage typing was 88%, pyocin typing 53% and reverse phage typing 62%. Typing with the DNA probe was not repeated. using polyclonal sera, repeated typing showed that 94% of the isolates were polyagglutinable. Using phage typing, 40% of the isolates belonged to phage type 31, while 60% were distributed amongst 32 phage types. Using monoclonal antibodies, 71% of the isolates belonged to 0-group 3, and these isolates showed 16 different phage types. Subdivision of the phage types was further achieved by both pyocin typing and reverse phage typing. The DNA probe typing made it possible in some cases to discriminate between isolates which were otherwise found identical with the conventional typing methods, while in other cases typing with the DNA probe recorded as identical isolates which conventional methods had typed as being different. These differences may be due to a high mutation rate caused by the selection pressure of antibiotics, and by the host immune response. According to our results, investigations of reproducibility and typability of old and new typing methods are essential when they are used in clinical situations. The low reproducibility of some of the typing methods in the present study affects the reliability of epidemiological investigations in cystic fibrosis patients. Usage of only one method may not be sufficient in cases of polyagglutinable strains from cystic fibrosis patients.

Published
1990

131. “Haemoxygenase-1 induction and exhaled markers of oxidative stress in lung diseases”, summary of the ERS Research Seminar in Budapest, Hungary, September, 1999

Author

Horváth, I., primary, MacNee, W., additional, Kelly, F.J., additional, Dekhuijzen, P.N.R., additional, Phillips, M., additional, Döring, G., additional, Choi, A.M.K., additional, Yamaya, M., additional, Bach, F.H., additional, Willis, D., additional, Donnelly, L.E., additional, Chung, K.F., additional, and Barnes, P.J., additional

Published
2001
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138. MEKC of desmosine and isodesmosine in urine of chronic destructive lung disease patients

Author

Viglio, S., primary, Iadarola, P., additional, Lupi, A., additional, Trisolini, R., additional, Tinelli, C., additional, Balbi, B, additional, Grassi, V, additional, Worlitzsch, D, additional, Döring, G, additional, Meloni, F., additional, Meyer, K.c, additional, Dowson, L, additional, Hill, S.l, additional, Stockley, R.a, additional, and Luisetti, M., additional

Published
2000
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140. A randomized double blind, placebo controlled phase 2 trial of BIIL 284 BS (an LTB4 receptor antagonist) for the treatment of lung disease in children and adults with cystic fibrosis.

Author

Konstan, M.W., Döring, G., Heltshe, S.L., Lands, L.C., Hilliard, K.A., Koker, P., Bhattacharya, S., Staab, A., and Hamilton, A.

Subjects
*CLINICAL trials, *PLACEBOS, *CYSTIC fibrosis, *JUVENILE diseases, *LUNG disease treatment, *INFLAMMATION, *PATIENTS
Abstract

Abstract: Background: Airway inflammation, mediated in part by LTB4, contributes to lung destruction in patients with cystic fibrosis (CF). LTB4-receptor inhibition may reduce airway inflammation. We report the results of a randomized, double-blind, placebo-controlled study of the efficacy and safety of the leukotriene B4 (LTB4)-receptor antagonist BIIL 284 BS in CF patients. Methods: CF patients aged ≥6years with mild to moderate lung disease were randomized to oral BIIL 284 BS or placebo once daily for 24weeks. Co-primary endpoints were change in FEV1 and incidence of pulmonary exacerbation. Results: After 420 (155 children, 265 adults) of the planned 600 patients were randomized, the trial was terminated after a planned interim analysis revealed a significant increase in pulmonary related serious adverse events (SAEs) in adults receiving BIIL 284 BS. Final analysis revealed SAEs in 36.1% of adults receiving BIIL 284 BS vs. 21.2% receiving placebo (p=0.007), and in 29.6% of children receiving BIIL 284 BS vs. 22.9% receiving placebo (p=0.348). In adults, the incidence of protocol-defined pulmonary exacerbation was greater in those receiving BIIL 284 BS than in those receiving placebo (33.1% vs. 18.2% respectively; p=0.005). In children, the incidence of protocol-defined pulmonary exacerbation was 19.8% in the BIIL 284 BS arm, and 25.7% in the placebo arm (p=0.38). Conclusions: While the cause of increased SAEs and exacerbations due to BIIL 284 BS is unknown, the outcome of this trial provides a cautionary tale for the administration of potent anti-inflammatory compounds to individuals with chronic infections, as the potential to significantly suppress the inflammatory response may increase the risk of infection-related adverse events. [Copyright &y& Elsevier]

Published
2014
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