120 results on '"Cho, Yong-Joon"'
Search Results
102. Genome sequence of the chromate-resistant bacterium Leucobacter salsicius type strain M1-8.
- Author
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Yun, Ji-Hyun, Cho, Yong-Joon, Chun, Jongsik, Hyun, Dong-Wook, and Bae, Jin-Woo
- Subjects
- *
GENOMES , *MICROBACTERIACEAE , *ACTINOMYCETALES , *RNA , *GENES - Abstract
Leucobacter salsicius M1-8 is a member of the Microbacteriaceae family within the class Actinomycetales. This strain is a Gram-positive, rod-shaped bacterium and was previously isolated from a Korean fermented food. Most members of the genus Leucobacter are chromate-resistant and this feature could be exploited in biotechnological applications. However, the genus Leucobacter is poorly characterized at the genome level, despite its potential importance. Thus, the present study determined the features of Leucobacter salsicius M1-8, as well as its genome sequence and annotation. The genome comprised 3,185,418 bp with a G+C content of 64.5%, which included 2,865 protein-coding genes and 68 RNA genes. This strain possessed two predicted genes associated with chromate resistance, which might facilitate its growth in heavy metal-rich environments. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
103. Tat-mediated protein transduction of human brain pyridoxal kinase into PC12 cells
- Author
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Kim, Dae Won, primary, Kim, Chung Kwon, additional, Choi, Soo Hyun, additional, Choi, Hee Soon, additional, Kim, So Young, additional, An, Jae Jin, additional, Lee, Seung Ree, additional, Lee, Sun Hwa, additional, Kwon, Oh-Shin, additional, Kang, Tae-Cheon, additional, Won, Moo Ho, additional, Cho, Yong Joon, additional, Cho, Sung-Woo, additional, Kang, Jung Hoon, additional, Kim, Tae Yoon, additional, Lee, Kil Soo, additional, Park, Jinseu, additional, Eum, Won Sik, additional, and Choi, Soo Young, additional
- Published
- 2005
- Full Text
- View/download PDF
104. Differential expression and role of two dithiol glutaredoxins Grx1 and Grx2 in Schizosaccharomyces pombe
- Author
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Chung, Woo-Hyun, primary, Kim, Kyoung-Dong, additional, Cho, Yong-Joon, additional, and Roe, Jung-Hye, additional
- Published
- 2004
- Full Text
- View/download PDF
105. A Vanillin Derivative Causes Mitochondrial Dysfunction and Triggers Oxidative Stress in Cryptococcus neoformans.
- Author
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Kim, Jin Hyo, Lee, Han-Ok, Cho, Yong-Joon, Kim, Jeongmi, Chun, Jongsik, Choi, Jaehyuk, Lee, Younghoon, and Jung, Won Hee
- Subjects
VANILLIN ,MITOCHONDRIAL pathology ,OXIDATIVE stress ,CRYPTOCOCCUS neoformans ,ANTIOXIDANTS ,ANTIMUTAGENS ,PATHOGENIC fungi ,MENINGITIS - Abstract
Vanillin is a well-known food and cosmetic additive and has antioxidant and antimutagenic properties. It has also been suggested to have antifungal activity against major human pathogenic fungi, although it is not very effective. In this study, the antifungal activities of vanillin and 33 vanillin derivatives against the human fungal pathogen Cryptococcus neoformans, the main pathogen of cryptococcal meningitis in immunocompromised patients, were investigated. We found a structural correlation between the vanillin derivatives and antifungal activity, showing that the hydroxyl or alkoxy group is more advantageous than the halogenated or nitrated group in benzaldehyde. Among the vanillin derivatives with a hydroxyl or alkoxy group, o-vanillin and o-ethyl vanillin showed the highest antifungal activity against C. neoformans. o-Vanillin was further studied to understand the mechanism of antifungal action. We compared the transcriptome of C. neoformans cells untreated or treated with o-vanillin by using RNA sequencing and found that the compound caused mitochondrial dysfunction and triggered oxidative stress. These antifungal mechanisms of o-vanillin were experimentally confirmed by the significantly reduced growth of the mutants lacking the genes involved in mitochondrial functions and oxidative stress response. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
106. Phenidone attenuates oxygen/glucose deprivation-induced neurotoxicity by antioxidant and antiapoptotic action in mouse cortical cultures
- Author
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Wie, Myung Bok, primary, Cho, Yong Joon, additional, Jhoo, Wang Kee, additional, and Kim, Hyoung Chun, additional
- Published
- 1999
- Full Text
- View/download PDF
107. An outbreak of rabbit sudden death in Korea suspected of a new viral hepatitis.
- Author
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LEE, Cha Soo, primary, PARK, Cheong Kyu, additional, SHIN, Tae Kyun, additional, CHO, Yong Joon, additional, and JYEONG, Jong Sik, additional
- Published
- 1990
- Full Text
- View/download PDF
108. Draft genome sequence of Pseudomonassp. strain G5, isolated from a traditional indigo fermentation dye vat
- Author
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Park, Sunhwa, Lee, Ji-Hoon, Cho, Yong-Joon, Chun, Jongsik, and Hur, Hor-Gil
- Abstract
In previous study, alkaliphilic and thermotolerant bacterium strain, Pseudomonassp. strain G5, capable of reducing insoluble indigo was isolated from Korean traditional fermentation liquor. Here, we report the draft genome sequence and annotation of strain G5 to provide the genomic information involved in indigo reduction process.
- Published
- 2013
- Full Text
- View/download PDF
109. A Novel Virus Alters Gene Expression and Vacuolar Morphology in MalasseziaCells and Induces a TLR3-Mediated Inflammatory Immune Response
- Author
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Park, Minji, Cho, Yong-Joon, Kim, Donggyu, Yang, Chul-Su, Lee, Shi Mun, Dawson, Thomas L., Nakamizo, Satoshi, Kabashima, Kenji, Lee, Yang Won, and Jung, Won Hee
- Abstract
Malasseziais the most dominant fungal genus on the human skin surface and is associated with various skin diseases including dandruff and seborrheic dermatitis. Among Malasseziaspecies, Malassezia restrictais the most widely observed species on the human skin. In the current study, we identified a novel dsRNA virus, named MrV40, in M. restrictaand characterized the sequence and structure of the viral genome along with an independent satellite dsRNA viral segment. Moreover, expression of genes involved in ribosomal synthesis and programmed cell death was altered, indicating that virus infection affected the physiology of the fungal host cells. Our data also showed that the viral nucleic acid from MrV40 induces a TLR3-mediated inflammatory immune response in bone marrow-derived dendritic cells, indicating that a viral element likely contributes to the pathogenicity of Malassezia. This is the first study to identify and characterize a novel mycovirus in Malassezia.
- Published
- 2020
- Full Text
- View/download PDF
110. The Genome Sequence of ‘Mycobacterium massiliense’ Strain CIP 108297 Suggests the Independent Taxonomic Status of the Mycobacterium abscessus Complex at the Subspecies Level.
- Author
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Cho, Yong-Joon, Yi, Hana, Chun, Jongsik, Cho, Sang-Nae, Daley, Charles L., Koh, Won-Jung, and Jae Shin, Sung
- Subjects
- *
MYCOBACTERIUM , *RIBOSOMAL RNA , *NUCLEOTIDE sequence , *PATHOGENIC microorganisms , *PHYLOGENY , *SCIENTIFIC observation , *SUBSPECIES - Abstract
Members of the Mycobacterium abscessus complex are rapidly growing mycobacteria that are emerging as human pathogens. The M. abscessus complex was previously composed of three species, namely M. abscessussensu stricto, ‘M. massiliense’, and ‘M. bolletii’. In 2011, ‘M. massiliense’ and ‘M. bolletii’ were united and reclassified as a single subspecies within M. abscessus: M. abscessus subsp. bolletii. However, the placement of ‘M. massiliense’ within the boundary of M. abscessus subsp. bolletii remains highly controversial with regard to clinical aspects. In this study, we revisited the taxonomic status of members of the M. abscessus complex based on comparative analysis of the whole-genome sequences of 53 strains. The genome sequence of the previous type strain of ‘Mycobacterium massiliense’ (CIP 108297) was determined using next-generation sequencing. The genome tree based on average nucleotide identity (ANI) values supported the differentiation of ‘M. bolletii’ and ‘M. massiliense’ at the subspecies level. The genome tree also clearly illustrated that ‘M. bolletii’ and ‘M. massiliense’ form a distinct phylogenetic clade within the radiation of the M. abscessus complex. The genomic distances observed in this study suggest that the current M. abscessus subsp. bolletii taxon should be divided into two subspecies, M. abscessus subsp. massiliense subsp. nov. and M. abscessus subsp. bolletii, to correspondingly accommodate the previously known ‘M. massiliense’ and ‘M. bolletii’ strains. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
111. A Vanillin Derivative Causes Mitochondrial Dysfunction and Triggers Oxidative Stress in Cryptococcus neoformans.
- Author
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Kim, Jin Hyo, Lee, Han-Ok, Cho, Yong-Joon, Kim, Jeongmi, Chun, Jongsik, Choi, Jaehyuk, Lee, Younghoon, and Jung, Won Hee
- Subjects
- *
VANILLIN , *MITOCHONDRIAL pathology , *OXIDATIVE stress , *CRYPTOCOCCUS neoformans , *ANTIOXIDANTS , *ANTIMUTAGENS , *PATHOGENIC fungi , *MENINGITIS - Abstract
Vanillin is a well-known food and cosmetic additive and has antioxidant and antimutagenic properties. It has also been suggested to have antifungal activity against major human pathogenic fungi, although it is not very effective. In this study, the antifungal activities of vanillin and 33 vanillin derivatives against the human fungal pathogen Cryptococcus neoformans, the main pathogen of cryptococcal meningitis in immunocompromised patients, were investigated. We found a structural correlation between the vanillin derivatives and antifungal activity, showing that the hydroxyl or alkoxy group is more advantageous than the halogenated or nitrated group in benzaldehyde. Among the vanillin derivatives with a hydroxyl or alkoxy group, o-vanillin and o-ethyl vanillin showed the highest antifungal activity against C. neoformans. o-Vanillin was further studied to understand the mechanism of antifungal action. We compared the transcriptome of C. neoformans cells untreated or treated with o-vanillin by using RNA sequencing and found that the compound caused mitochondrial dysfunction and triggered oxidative stress. These antifungal mechanisms of o-vanillin were experimentally confirmed by the significantly reduced growth of the mutants lacking the genes involved in mitochondrial functions and oxidative stress response. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
112. Influence of oipA Phase Variation on Virulence Phenotypes Related to Type IV Secretion System in Helicobacter pylori.
- Author
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Lai J, Angulmaduwa S, Kim MA, Kim A, Tissera K, Cho YJ, and Cha JH
- Subjects
- Virulence genetics, Humans, Virulence Factors genetics, Virulence Factors metabolism, Phenotype, Antigens, Bacterial genetics, Antigens, Bacterial metabolism, Bacterial Proteins genetics, Bacterial Proteins metabolism, Helicobacter pylori genetics, Helicobacter pylori pathogenicity, Helicobacter pylori physiology, Type IV Secretion Systems genetics, Type IV Secretion Systems metabolism, Interleukin-8 metabolism, Interleukin-8 genetics, Bacterial Outer Membrane Proteins genetics, Bacterial Outer Membrane Proteins metabolism, Helicobacter Infections microbiology
- Abstract
Background: oipA, an outer membrane protein of Helicobacter pylori, is linked to IL-8 induction and gastric inflammation, but its role is debated due to inconsistent findings. This study aims to explore the role of oipA phase variation in modulating the virulence traits of H. pylori, a bacterium strongly associated with the development of gastric cancer., Material and Methods: American clinical isolate AH868 strain for naturally occurring phase variations of the oipA gene, and G27 strain for in vitro-induced phase variations were used to elucidate oipA's impact on key virulence phenotypes, including cell elongation, CagA phosphorylation, and IL-8 induction., Results: Using AH868 strain, natural oipA phase variation does not affect cell elongation and IL-8 induction. Interestingly, however, in vitro-induced oipA phase variations in G27 strain uncovered that 9.4% of oipA "Off" transformants exhibit reduced cell elongation while all maintaining consistent IL-8 induction levels. Additionally, complementation of oipA "Off to On" status restores the cell elongation phenotype in 12.5% of transformants, highlighting the importance of oipA in maintaining normal cell morphology. Crucially, these variations in cell elongation are not linked to changes in bacterial adherence capabilities. Furthermore, the study shows a correlation among oipA phase variation, cell elongation, and CagA phosphorylation, suggesting that oipA influences the functionality of the Type IV secretion system. Whole-genome sequencing of selected transformants reveals genetic variations in bab paralogue, cagY gene, and other genomic regions, underscoring the complex genetic interactions that shape H. pylori's virulence., Conclusions: Our research provides new insights into the subtle yet significant role of oipA phase variation in H. pylori pathogenicity, emphasizing the need for further studies to explore the intricate molecular mechanisms involved. This understanding could pave the way for targeted therapeutic strategies to mitigate the impact of H. pylori on human health., (© 2024 The Author(s). Helicobacter published by John Wiley & Sons Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
113. Ctr9 promotes virulence of Candida albicans by regulating methionine metabolism.
- Author
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Park J, Park S, Kim J, Cho YJ, and Lee JS
- Subjects
- Animals, Mice, Virulence, Mice, Inbred BALB C, Female, RAW 264.7 Cells, Hyphae growth & development, Hyphae genetics, Hyphae metabolism, Gene Expression Profiling, Candida albicans pathogenicity, Candida albicans genetics, Candida albicans metabolism, Fungal Proteins genetics, Fungal Proteins metabolism, Methionine metabolism, Candidiasis microbiology, Macrophages microbiology, Gene Expression Regulation, Fungal
- Abstract
Candida albicans , a part of normal flora, is an opportunistic fungal pathogen and causes severe health issues in immunocompromised patients. Its pathogenicity is intricately linked to the transcriptional regulation of its metabolic pathways. Paf1 complex (Paf1C) is a crucial transcriptional regulator that is highly conserved in eukaryotes. The objective of this study was to explore the role of Paf1C in the metabolic pathways and how it influences the pathogenicity of C. albicans . Paf1C knockout mutant strains of C. albicans ( ctr9 Δ/Δ, leo1 Δ/Δ, and cdc73 Δ/Δ) were generated using the CRISPR-Cas9 system. To investigate the effect of Paf1C on pathogenicity, macrophage interaction assays and mouse survival tests were conducted. The growth patterns of the Paf1C knockout mutants were analyzed through spotting assays and growth curve measurements. Transcriptome analysis was conducted under yeast conditions (30°C without serum) and hyphal conditions (37°C with 10% FBS), to further elucidate the role of Paf1C in the pathogenicity of C. albicans . CTR9 deletion resulted in the attenuation of C. albicans virulence, in macrophage and mouse models. Furthermore, we confirmed that the reduced virulence of the ctr9 Δ/Δ mutant can be attributed to a decrease in C. albicans cell abundance. Moreover, transcriptome analysis revealed that metabolic processes required for cell proliferation are impaired in ctr9 Δ/Δ mutant. Notably, CTR9 deletion led to the downregulation of methionine biosynthetic genes and the cAMP-PKA signaling pathway-related hypha essential genes, which are pivotal for virulence. Our results suggest that Ctr9-regulated methionine metabolism is a crucial factor for determining C. albicans pathogenicity.
- Published
- 2024
- Full Text
- View/download PDF
114. A shared mechanism of multidrug resistance in laboratory-evolved uropathogenic Escherichia coli .
- Author
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Choi N, Choi E, Cho YJ, Kim MJ, Choi HW, and Lee EJ
- Subjects
- Animals, Mice, Escherichia coli Proteins genetics, Escherichia coli Proteins metabolism, Female, Humans, Microbial Sensitivity Tests, Whole Genome Sequencing, Uropathogenic Escherichia coli genetics, Uropathogenic Escherichia coli drug effects, Drug Resistance, Multiple, Bacterial genetics, Urinary Tract Infections microbiology, Escherichia coli Infections microbiology, Anti-Bacterial Agents pharmacology, Ampicillin pharmacology, Mutation
- Abstract
The emergence of multidrug-resistant bacteria poses a significant threat to human health, necessitating a comprehensive understanding of their underlying mechanisms. Uropathogenic Escherichia coli (UPEC), the primary causative agent of urinary tract infections, is frequently associated with multidrug resistance and recurrent infections. To elucidate the mechanism of resistance of UPEC to beta-lactam antibiotics, we generated ampicillin-resistant UPEC strains through continuous exposure to low and high levels of ampicillin in the laboratory, referred to as Low Amp
R and High AmpR , respectively. Whole-genome sequencing revealed that both Low and High AmpR strains contained mutations in the marR , acrR , and envZ genes. The High AmpR strain exhibited a single additional mutation in the nlpD gene. Using protein modeling and qRT-PCR analyses, we validated the contributions of each mutation in the identified genes to antibiotic resistance in the AmpR strains, including a decrease in membrane permeability, increased expression of multidrug efflux pump, and inhibition of cell lysis. Furthermore, the AmpR strain does not decrease the bacterial burden in the mouse bladder even after continuous antibiotic treatment in vivo , implicating the increasing difficulty in treating host infections caused by the AmpR strain. Interestingly, ampicillin-induced mutations also result in multidrug resistance in UPEC, suggesting a common mechanism by which bacteria acquire cross-resistance to other classes of antibiotics.- Published
- 2024
- Full Text
- View/download PDF
115. N-terminal acetylation of Set1-COMPASS fine-tunes H3K4 methylation patterns.
- Author
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Woo H, Oh J, Cho YJ, Oh GT, Kim SY, Dan K, Han D, Lee JS, and Kim T
- Subjects
- Acetylation, Methylation, Protein Processing, Post-Translational, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae genetics, Multiprotein Complexes metabolism, Histone-Lysine N-Methyltransferase metabolism, Histone-Lysine N-Methyltransferase genetics, Histones metabolism, Saccharomyces cerevisiae Proteins metabolism, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins chemistry
- Abstract
H3K4 methylation by Set1-COMPASS (complex of proteins associated with Set1) is a conserved histone modification. Although it is critical for gene regulation, the posttranslational modifications of this complex that affect its function are largely unexplored. This study showed that N-terminal acetylation of Set1-COMPASS proteins by N-terminal acetyltransferases (NATs) can modulate H3K4 methylation patterns. Specifically, deleting NatA substantially decreased global H3K4me3 levels and caused the H3K4me2 peak in the 5' transcribed regions to shift to the promoters. NatA was required for N-terminal acetylation of three subunits of Set1-COMPASS: Shg1, Spp1, and Swd2. Moreover, deleting Shg1 or blocking its N-terminal acetylation via proline mutation of the target residue drastically reduced H3K4 methylation. Thus, NatA-mediated N-terminal acetylation of Shg1 shapes H3K4 methylation patterns. NatB also regulates H3K4 methylation, likely via N-terminal acetylation of the Set1-COMPASS protein Swd1. Thus, N-terminal acetylation of Set1-COMPASS proteins can directly fine-tune the functions of this complex, thereby substantially shaping H3K4 methylation patterns.
- Published
- 2024
- Full Text
- View/download PDF
116. Draft genome sequence of Mycobacterium abscessus subsp. bolletii BD(T).
- Author
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Choi GE, Cho YJ, Koh WJ, Chun J, Cho SN, and Shin SJ
- Subjects
- Molecular Sequence Data, Mycobacterium classification, Genome, Bacterial, Mycobacterium genetics
- Abstract
Mycobacterium abscessus subsp. bolletii is an increasing cause of human pulmonary disease and infections of the skin and soft tissues. Consistent reports of human infections indicate that M. bolletii is a highly pathogenic, emerging species of rapidly growing mycobacteria (RGM). Here we report the first whole-genome sequence of M. abscessus subsp. bolletii BD(T).
- Published
- 2012
- Full Text
- View/download PDF
117. Genome sequence of Escherichia coli AA86, isolated from cow feces.
- Author
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Yi H, Cho YJ, Hur HG, and Chun J
- Subjects
- Animals, Base Sequence, Cattle, DNA, Bacterial genetics, Escherichia coli classification, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Escherichia coli genetics, Escherichia coli isolation & purification, Feces microbiology, Genome, Bacterial
- Abstract
Escherichia coli AA86 (=KACC 15541) is an enteric bacterium that was isolated from a sample of healthy cow feces. Its genome sequence revealed that it is most closely related to the human fecal strain E. coli SE15 and could be classified under E. coli phylogenetic group B2. Here, we report the genome sequence of E. coli AA86, consisting of 3 contigs and 2 plasmids.
- Published
- 2011
- Full Text
- View/download PDF
118. Complete genome sequence of Vibrio vulnificus MO6-24/O.
- Author
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Park JH, Cho YJ, Chun J, Seok YJ, Lee JK, Kim KS, Lee KH, Park SJ, and Choi SH
- Subjects
- Carbohydrate Metabolism genetics, Humans, Interspersed Repetitive Sequences, Molecular Sequence Data, Sepsis microbiology, Sequence Analysis, DNA, Vibrio vulnificus isolation & purification, DNA, Bacterial chemistry, DNA, Bacterial genetics, Genome, Bacterial, Vibrio vulnificus genetics
- Abstract
Vibrio vulnificus is the causative agent of life-threatening septicemia and severe wound infections. Here, we announce the complete annotated genome sequence of V. vulnificus MO6-24/O, isolated from a patient with septicemia. When it is compared with previously known V. vulnificus genomes, the genome of this bacterium shows a unique genetic makeup, including phagelike elements, carbohydrate metabolism-related genes, and the superintegron.
- Published
- 2011
- Full Text
- View/download PDF
119. Synthesis of gamma-glutamylcysteine as a major low-molecular-weight thiol in lactic acid bacteria Leuconostoc spp.
- Author
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Kim EK, Cha CJ, Cho YJ, Cho YB, and Roe JH
- Subjects
- Dipeptides analysis, Dipeptides genetics, Gene Expression, Genes, Bacterial, Hydrogen Peroxide pharmacology, Lactic Acid metabolism, Leuconostoc drug effects, Leuconostoc genetics, Molecular Weight, Oxidative Stress, Promoter Regions, Genetic, RNA, Messenger metabolism, Single-Strand Specific DNA and RNA Endonucleases chemistry, Transcription Initiation Site, Bacterial Proteins genetics, Dipeptides biosynthesis, Glutamate-Cysteine Ligase genetics, Leuconostoc enzymology, Sulfhydryl Compounds metabolism
- Abstract
Some members of lactic acid bacteria are known to synthesize glutathione (GSH) or to import it from growth medium, whereas others are not. Analysis of the genome sequences of several Leuconostoc spp. indicate the presence of the gene gshA that encodes gamma-glutamylcysteine synthetase, but not the gene gshB encoding glutathione synthetase. We report here that, in cells of Leuconostoc kimchii and Leuconostoc mesenteroides, gamma-glutamylcysteine (gamma-GC) is present in large amount, whereas GSH is not detectable. The level of gamma-GC was higher at the stationary phase than at the exponential phase. Expression of the gshA gene in Leuconostoc spp. analyzed by S1 mapping showed the increased mRNA level upon hydrogen peroxide treatment. From high-resolution S1 mapping, the transcriptional start site was mapped and the putative promoter elements were suggested. This work suggests that gamma-GC has a significant role in Leuconostoc spp. as the major low-molecular-weight thiol.
- Published
- 2008
- Full Text
- View/download PDF
120. Enhanced transduction of Cu,Zn-superoxide dismutase with HIV-1 Tat protein transduction domains at both termini.
- Author
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Eum WS, Jang SH, Kim DW, Choi HS, Choi SH, Kim SY, An JJ, Lee SH, Han K, Kang JH, Kang TC, Won MH, Cho YJ, Choi JH, Kim TY, Park J, and Choi SY
- Subjects
- Animals, Cell Survival, Escherichia coli genetics, Escherichia coli metabolism, HeLa Cells, Humans, Mice, Plasmids, Protein Structure, Tertiary, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins isolation & purification, Recombinant Fusion Proteins metabolism, Skin cytology, Superoxide Dismutase genetics, beta-Galactosidase metabolism, Gene Products, tat metabolism, Skin metabolism, Superoxide Dismutase metabolism, Transduction, Genetic
- Abstract
The human immunodeficiency virus type 1 (HIV-1) Tat protein transduction domain (PTD) is responsible for highly efficient protein transduction across plasma membranes. In a previous study, we showed that Tat-Cu,Zn-superoxide dismutase (Tat-SOD) can be directly transduced into mammalian cells across the lipid membrane barrier. In this study, we fused the human SOD gene with a Tat PTD transduction vector at its N- and/or C-terminus. The fusion proteins (Tat-SOD, SOD-Tat, Tat-SOD-Tat) were purified from Escherichia coli and their ability to enter cells in vitro and in vivo compared by Western blotting and immunohistochemistry. The transduction efficiencies and biological activities of the SOD fusion protein with the Tat PTD at either terminus were equivalent and lower than the fusion protein with the Tat PTD at both termini. The availability of a more efficient SOD fusion protein provides a powerful vehicle for therapy in human diseases related to this anti-oxidant enzyme and to reactive oxygen species.
- Published
- 2005
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