416 results on '"Calza, S"'
Search Results
102. Filtering genes to improve sensitivity in oligonucleotide microarray data analysis
- Author
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Calza, S., primary, Raffelsberger, W., additional, Ploner, A., additional, Sahel, J., additional, Leveillard, T., additional, and Pawitan, Y., additional
- Published
- 2007
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103. Low brain‐derived neurotrophic factor (BDNF) levels in serum of Huntington's disease patients
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Ciammola, A., primary, Sassone, J., additional, Cannella, M., additional, Calza, S., additional, Poletti, B., additional, Frati, L., additional, Squitieri, F., additional, and Silani, V., additional
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- 2007
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104. Offspring sexual dimorphism and sex‐allocation in relation to parental age and paternal ornamentation in the barn swallow
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Saino, N., primary, Ambrosini, R., additional, Martinelli, R., additional, Calza, S., additional, Møller, A. P., additional, and Pilastro, A., additional
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- 2002
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105. What do professionals need for an Open-Paediatric Intensive Care Unit (PICU)? A focus group study on PICU professionals.
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Bagnasco, A., Calza, S., Costa, M., Rosa, F., and Sasso, L.
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INTENSIVE care units , *CRITICALLY ill children , *MEDICAL personnel - Abstract
Background: PICU is a complex stressful, high-tech environment for professionals, parents and critically ill children. Literature suggests that knowing the context is crucial in determine the suitable interventions to act. Professionals' needs in the Italian context need to be investigated. The aim of the study was to highlight the healthcare professionals' perceptions of their needs regarding the concept of Open PICUs and the presence of parents during their child's stay in the PICU, without highly restricted visiting policies. Methods: Qualitative approach (multi-professional focus group) was used. The transcription and categorization followed the principles of "content analysis". Criteria concerning validity, reliability, confirmability and replicability have been satisfied. Results: Results identified the following 4 main categories of needs, which were considered important by the participants: communication skills, education on the centrality of parents; the organization of the presence of parents, and suitable spaces. The majority of the participants viewed Open PICUs positively but need an improvement of: communication skills, education regarding "centredness of the parent", a better organization of the presence of the parent and suitable spaces for the parent in the Unit. Conclusions: Our study shows that focus group approach is useful to gain qualitative data in a PICU. Strategic health care plans ought to focus, much more than in the past, on models that allow families to stay close to their children during the most severe stages of their illness. The Family Centred Care model is not easy to implement in settings with an intensive use of technological equipment. Open PICUs are not just places where parents have more time to see their children, but require a profound transformation into a place where health professionals and technical competences, join with relational competences to achieve family empowerment. Open PICUs would be pointless, or even counterproductive, if they were not supported by improvement and major awareness of the scope of ethical practice, with a view to provide a full and competent coverage of the clinical, ethical and relational issues relating to children and their parents, even in end-of-life situations. [ABSTRACT FROM AUTHOR]
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- 2013
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106. Clinical features and prognosis of patients with renal cancer and a second malignancy.
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Antonelli A, Calza S, Arrighi N, Zani D, Corti S, Cozzoli A, Zanotelli T, Cosciani Cunico S, and Simeone C
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- 2012
107. Effects of a dipteran ectoparasite on immune response and growth trade-offs in barn swallow, Hirundo rustica, nestlings
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Moller, A. P., Saino, N., and Calza, S.
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IMMUNOLOGY ,PARASITOLOGY ,HOST-parasite relationships ,BIRD behavior - Abstract
Parasites can have a profound effect on biology and evolution of thehosts, which are expected to have evolved physiological and developmental mechanisms that allow them to minimise the costs imposed by parasites. In this study we analyse the effects of a dipteran ectoparasite on barn swallow (Hirundo rustica) nestling biology including rate of somatic growth, plasma protein concentration, blood cell sedimentation rate, hematocrit, concentration of leukocytes in peripheral blood, and T-lymphocyte cell-mediated immunocompetence. In a natural population, intensity of parasite infestation was positively correlated with growth of feathers. Nestlings in heavily infested nests may decide to allocate more resources to feather growth thus fledging early. To test this hypothesis, the detrimental effects of parasites on nestlings, and the existence of trade-offs between competing growth processes, we inoculated some nests with additional flies. Nestlings exposed to increased infestation had larger rate of feather growth but werein poorer condition than unmanipulated controls. Parasite inoculation resulted in larger concentrations of eosinophils and lymphocytes. Among siblings of broods inoculated with parasites, those that had thelargest rate of feather growth had the lowest rate of increase in tarsus length and body mass. We conclude that louse flies depress barn swallow nestling condition and influence their immune profile. However, they also enhance growth of a morphological character that may allow nestlings to reduce the impact of parasites. Nestlings apparently experience a trade-off between the competing demands for growing feathers and other somatic characters. [ABSTRACT FROM AUTHOR]
- Published
- 1998
108. Barriers to nursing care of foreign patients in a pediatric stem cell transplantation sector: A qualitative study | Barriere all'assistenza infermieristica dei pazienti stranieri in un settore di trapianto di cellule staminali pediatrico: Uno studio qualitativo
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Calza, S., Rossi, S., Annamaria Bagnasco, and Sasso, L.
109. Effects of a dipteran ectoparasite on immune response and growth trade-offs in barn swallow, Hirundo rustica, nestlings
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Nicola Saino, Calza, S., and Møller, A. P.
110. Comprehensive landscape of subtype-specific coding and noncoding RNA transcripts in breast cancer
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Vu, T. N., Setia Pramana, Calza, S., Suo, C., Lee, D., and Pawitan, Y.
111. Che cosa c'è di nuovo nell'accoglienza del paziente straniero pediatrico
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Calza, S., Rossi, S., Bagnasco, A., and LOREDANA SASSO
112. Barriere all'assistenza infermieristica dei pazienti stranieri in un settore di trapianto di cellule staminali pediatrico: uno studio qualitativo.
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Calza, S., Rossi, S., Bagnasco, A., and Sasso, L.
- Published
- 2012
113. 919P Predictive multi-omic signature in locally advanced laryngeal/hypopharyngeal (LH) squamous cell carcinoma (SCC) treated with induction chemotherapy (IC).
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Bossi, P., Mattavelli, D., Chiocca, S., Gurizzan, C., Compagnoni, M., Dietz, A., Hovig, E., Kalogerini, M., Khelik, K., Lorini, L., Mesia Nin, R., Plana Serrahima, M., Ravanelli, M., Rondi, P., Smussi, D., Wichmann, G., Yinxhiu, Z., and Calza, S.
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INDUCTION chemotherapy , *SQUAMOUS cell carcinoma - Published
- 2024
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114. miRNome Profiling Detects miR-101-3p and miR-142-5p as Putative Blood Biomarkers of Frailty Syndrome
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Giulia Carini, Jessica Mingardi, Francesco Bolzetta, Alberto Cester, Andrea Bolner, Giampietro Nordera, Luca La Via, Alessandro Ieraci, Isabella Russo, Stefania Maggi, Stefano Calza, Maurizio Popoli, Nicola Veronese, Laura Musazzi, Alessandro Barbon, Carini, G., Mingardi, J., Bolzetta, F., Cester, A., Bolner, A., Nordera, G., Via, L.L., Ieraci, A., Russo, I., Maggi, S., Calza, S., Popoli, M., Veronese, N., Musazzi, L., Barbon, A., Carini, G, Mingardi, J, Bolzetta, F, Cester, A, Bolner, A, Nordera, G, Via, L, Ieraci, A, Russo, I, Maggi, S, Calza, S, Popoli, M, Veronese, N, Musazzi, L, and Barbon, A
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smRNA-seq ,microRNA ,Frailty ,miR-142-5p ,Biomarkers ,MicroRNA ,MiR-101-3p ,MiR-142-5p ,MiRNome ,RNA-seq ,SmRNA-seq ,biomarkers ,miRNome ,Real-Time Polymerase Chain Reaction ,frailty ,miR-101-3p ,MicroRNAs ,Genetics ,biomarker ,Humans ,Genetics (clinical) - Abstract
Frailty is an aging-related pathology, defined as a state of increased vulnerability to stressors, leading to a limited capacity to meet homeostatic demands. Extracellular microRNAs (miRNAs) were proposed as potential biomarkers of various disease conditions, including age-related pathologies. The primary objective of this study was to identify blood miRNAs that could serve as potential biomarkers and candidate mechanisms of frailty. Using the Fried index, we enrolled 22 robust and 19 frail subjects. Blood and urine samples were analysed for several biochemical parameters. We observed that sTNF-R was robustly upregulated in the frail group, indicating the presence of an inflammatory state. Further, by RNA-seq, we profiled 2654 mature miRNAs in the whole blood of the two groups. Expression levels of selected differentially expressed miRNAs were validated by qPCR, and target prediction analyses were performed for the dysregulated miRNAs. We identified 2 miRNAs able to significantly differentiate frail patients from robust subjects. Both miR-101-3p and miR-142-5p were found to be downregulated in the frail vs. robust group. Finally, using bioinformatics targets prediction tools, we explored the potential molecular mechanisms and cellular pathways regulated by the two miRNAs and potentially involved in frailty. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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- 2022
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115. Trop-2 overexpression as an independent marker for poor overall survival in ovarian carcinoma patients.
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Bignotti E, Todeschini P, Calza S, Falchetti M, Ravanini M, Tassi RA, Ravaggi A, Bandiera E, Romani C, Zanotti L, Tognon G, Odicino FE, Facchetti F, Pecorelli S, and Santin AD
- Abstract
BACKGROUND: Prognostic factors currently available are insufficient to predict the clinical course of epithelial ovarian cancer (EOC). In a previous microarray study we identified the human trophoblast cell surface antigen Trop-2 as one of the top differentially expressed genes in serous papillary EOCs compared to normal human ovarian surface epithelial (HOSE) short-term cultures. The aim of the present investigation was to analyse Trop-2 expression at mRNA and protein level and to assess its prognostic significance in EOC. METHODS: Using quantitative real-time PCR we tested a total of 104 fresh-frozen EOC tissues and 24 HOSE for Trop-2 mRNA expression. Trop-2 protein expression was then examined by immunohistochemistry in matched formalin-fixed paraffin-embedded EOC samples and in 13 normal ovaries. Finally, we correlated Trop-2 expression to EOC conventional clinicopathological features and patient outcomes. RESULTS: We found a significant Trop-2 mRNA and protein upregulation in EOCs compared to normal controls (p<0.001). Trop-2 protein overexpression was significantly associated with the presence of ascites (p=0.04) and lymph node metastases (p=0.04). By univariate survival analysis, Trop-2 protein overexpression was significantly associated with decreased progression-free (p=0.02) and overall survival (p=0.01). Importantly, Trop-2 protein overexpression was an independent prognostic marker for shortened survival time in multivariate Cox regression analysis (p=0.04, HR=2.35, CI(95%)=1.03-5.34). CONCLUSIONS: Our results indicate, for the first time, that Trop-2 protein overexpression correlates with an aggressive malignant phenotype and may constitute a novel prognostic factor for EOC. The targeting of Trop-2 overexpression by immunotherapeutic strategies may represent an attractive and potentially effective approach in patients harbouring EOC. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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116. The chemerin/CMKLR1 axis regulates intestinal graft-versus-host disease
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Erica Dander, Paola Vinci, Stefania Vetrano, Camilla Recordati, Rocco Piazza, Grazia Fazio, Donatella Bardelli, Mattia Bugatti, Francesca Sozio, Andrea Piontini, Sonia Bonanomi, Luca Bertola, Elena Tassistro, Maria Grazia Valsecchi, Stefano Calza, William Vermi, Andrea Biondi, Annalisa Del Prete, Silvano Sozzani, Giovanna D’Amico, Dander, E, Vinci, P, Vetrano, S, Recordati, C, Piazza, R, Fazio, G, Bardelli, D, Bugatti, M, Sozio, F, Piontini, A, Bonanomi, S, Bertola, L, Tassistro, E, Valsecchi, M, Calza, S, Vermi, W, Biondi, A, Del Prete, A, Sozzani, S, and D'Amico, G
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immunology ,Chemokine ,Macrophage ,bone marrow transplantation ,chemokines ,General Medicine ,macrophages ,transplantation - Abstract
Gastrointestinal graft-versus-host disease (GvHD) is a major cause of mortality and morbidity following allogeneic bone marrow transplantation (allo-BMT). Chemerin is a chemotactic protein that recruits leukocytes to inflamed tissues by interacting with ChemR23/CMKLR1, a chemotactic receptor expressed by leukocytes, including macrophages. During acute GvHD, chemerin plasma levels were strongly increased in allo-BM-transplanted mice. The role of the chemerin/CMKLR1 axis in GvHD was investigated using Cmklr1-KO mice. WT mice transplanted with an allogeneic graft from Cmklr1-KO donors (t-KO) had worse survival and more severe GvHD. Histological analysis demonstrated that the gastrointestinal tract was the organ mostly affected by GvHD in t-KO mice. The severe colitis of t-KO mice was characterized by massive neutrophil infiltration and tissue damage associated with bacterial translocation and exacerbated inflammation. Similarly, Cmklr1- KO recipient mice showed increased intestinal pathology in both allogeneic transplant and dextran sulfate sodium-induced colitis. Notably, the adoptive transfer of WT monocytes into t-KO mice mitigated GvHD manifestations by decreasing gut inflammation and T cell activation. In patients, higher chemerin serum levels were predictive of GvHD development. Overall, these results suggest that CMKLR1/chemerin may be a protective pathway for the control of intestinal inflammation and tissue damage in GvHD.
- Published
- 2023
117. Transcranial Doppler as a screening test to exclude intracranial hypertension in brain-injured patients: the IMPRESSIT-2 prospective multicenter international study
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Frank A. Rasulo, Stefano Calza, Chiara Robba, Fabio Silvio Taccone, Daniele G. Biasucci, Rafael Badenes, Simone Piva, Davide Savo, Giuseppe Citerio, Jamil R. Dibu, Francesco Curto, Martina Merciadri, Paolo Gritti, Paola Fassini, Soojin Park, Massimo Lamperti, Pierre Bouzat, Paolo Malacarne, Arturo Chieregato, Rita Bertuetti, Raffaele Aspide, Alfredo Cantoni, Victoria McCredie, Lucrezia Guadrini, Nicola Latronico, Rasulo, F, Calza, S, Robba, C, Taccone, F, Biasucci, D, Badenes, R, Piva, S, Savo, D, Citerio, G, Dibu, J, Curto, F, Merciadri, M, Gritti, P, Fassini, P, Park, S, Lamperti, M, Bouzat, P, Malacarne, P, Chieregato, A, Bertuetti, R, Aspide, R, Cantoni, A, Mccredie, V, Guadrini, L, and Latronico, N
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Intracranial Pressure ,Ultrasonography, Doppler, Transcranial ,Doppler ,Brain ,Transcranial ,Critical Care and Intensive Care Medicine ,Settore MED/41 ,Humans ,Prospective Studies ,Brain injury ,Intracranial Hypertension ,Intracranial hypertension ,Intracranial pressure ,Noninvasive monitoring ,Ultrasonography - Abstract
Background Alternative noninvasive methods capable of excluding intracranial hypertension through use of transcranial Doppler (ICPtcd) in situations where invasive methods cannot be used or are not available would be useful during the management of acutely brain-injured patients. The objective of this study was to determine whether ICPtcd can be considered a reliable screening test compared to the reference standard method, invasive ICP monitoring (ICPi), in excluding the presence of intracranial hypertension. Methods This was a prospective, international, multicenter, unblinded, diagnostic accuracy study comparing the index test (ICPtcd) with a reference standard (ICPi), defined as the best available method for establishing the presence or absence of the condition of interest (i.e., intracranial hypertension). Acute brain-injured patients pertaining to one of four categories: traumatic brain injury (TBI), subarachnoid hemorrhage (SAH), intracerebral hemorrhage (ICH) or ischemic stroke (IS) requiring ICPi monitoring, were enrolled in 16 international intensive care units. ICPi measurements (reference test) were compared to simultaneous ICPtcd measurements (index test) at three different timepoints: before, immediately after and 2 to 3 h following ICPi catheter insertion. Sensitivity, specificity, positive (PPV) and negative predictive values (NPV) were calculated at three different ICPi thresholds (> 20, > 22 and > 25 mmHg) to assess ICPtcd as a bedside real-practice screening method. A receiver operating characteristic (ROC) curve analysis with the area under the curve (AUC) was used to evaluate the discriminative accuracy and predictive capability of ICPtcd. Results Two hundred and sixty-two patients were recruited for final analysis. Intracranial hypertension (> 22 mmHg) occurred in 87 patients (33.2%). The total number of paired comparisons between ICPtcd and ICPi was 687. The NPV was elevated (ICP > 20 mmHg = 91.3%, > 22 mmHg = 95.6%, > 25 mmHg = 98.6%), indicating high discriminant accuracy of ICPtcd in excluding intracranial hypertension. Concordance correlation between ICPtcd and ICPi was 33.3% (95% CI 25.6–40.5%), and Bland–Altman showed a mean bias of -3.3 mmHg. The optimal ICPtcd threshold for ruling out intracranial hypertension was 20.5 mmHg, corresponding to a sensitivity of 70% (95% CI 40.7–92.6%) and a specificity of 72% (95% CI 51.9–94.0%) with an AUC of 76% (95% CI 65.6–85.5%). Conclusions and relevance ICPtcd has a high NPV in ruling out intracranial hypertension and may be useful to clinicians in situations where invasive methods cannot be used or not available. Trial registration: NCT02322970.
- Published
- 2022
118. Clinical efficacy of Lactobacillus reuteri-containing lozenges in the supportive therapy of generalized periodontitis stage III and IV, grade C: 1-year results of a double-blind randomized placebo-controlled pilot study
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Enrico Gherlone, Magda Mensi, Eleonara Scotti, Veronica Cappa, Gianluca Garzetti, Maria Gabriella Grusovin, Simone Bossini, Stefano Calza, Grusovin, M. G., Bossini, S., Calza, S., Cappa, V., Garzetti, G., Scotti, E., Gherlone, E. F., and Mensi, M.
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Limosilactobacillus reuteri ,medicine.medical_specialty ,Lactobacillus reuteri ,Bleeding on probing ,Pilot Projects ,Placebo ,03 medical and health sciences ,0302 clinical medicine ,Double-Blind Method ,Maintenance therapy ,Internal medicine ,medicine ,Tooth loss ,Humans ,Periodontal Pocket ,Clinical efficacy ,Periodontitis ,Probiotics ,Adverse effect ,General Dentistry ,biology ,business.industry ,030206 dentistry ,medicine.disease ,biology.organism_classification ,Treatment Outcome ,Supportive psychotherapy ,030220 oncology & carcinogenesis ,medicine.symptom ,business - Abstract
Objective: The aim of this 12-month mono-centre double-blind randomized placebo-controlled clinical study was to evaluate the efficacy of Lactobacillus reuteri-containing lozenges during the supportive therapy of generalized periodontitis stage III and IV, grade C (GPIII-IVC) patients. Material and methods: Twenty treated GPIII-IVC patients were randomly divided into 2 groups. The test group received two 3-month-long administrations of L. reuteri (2 lozenges/day after brushing) with a 3-month washout period, while the control one received a placebo. Outcome measures were tooth survival, complications and adverse events, change in probing pockets depth (PPD), change in probing attachment level (PAL), presence of bleeding on probing (BOP) and patient’s evaluation of treatment. Measurements were collected at 3, 6, 9 and 12months. Results: At 1year, no dropout, tooth loss, complications or adverse event were recorded. Mean PPD and mean PAL and percentages of sites with BOP were statistically improved (p < 0.05) compared with baseline in both groups, while more PPD reduction at all time points (p < 0.05) and more PAL gain at 6months and more BOP reduction at 6 and 9months were found in the probiotic group (p < 0.05). Conclusions: Within the limitation of the study, the use of L. reuteri probiotics lozenges improved some clinical outcomes in treated GPIII-IVC patients during maintenance therapy. Studies with a larger number of patients are needed to confirm these data. Clinical relevance: The use of L. reuteri probiotic lozenges could be considered as an adjunct in the maintenance therapy of GPIII-IVC patients.
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- 2019
119. microRNAs as possible biomarkers of frailty and cognitive impairment: the MATCH-In project
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Giulia Carini, Jessica Mingardi, Luca La Via, Francesco, Bolzetta, Annalisa, Tessari, Alberto, Cester, Stefania, Maggi, Veronese, Nicola, Stefano Calza, Leonardo Elia, Fiorentini, Chiara, Laura, Musazzi, Alessandro, Ieraci, Maurizio, Popoli, Barbon, Alessandro, Carini, G, Mingardi, J, La Via, L, Bolzetta, F, Tessari, A, Cester, A, Maggi, S, Veronese, N, Calza, S, Elia, L, Fiorentini, C, Musazzi, L, Ieraci, A, Popoli, M, and Barbon, A
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Frailty syndrome ,miRNA - Published
- 2021
120. EFFECTS OF HIGH CONCENTRATIONS OF RESVERATROL ON HUMAN SPERM CRYOPRESERVATION
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E. Porcu, L. Notarangelo, L. Cipriani, G. Damiano, P. M. Ciotti, N. Calza, S. Zuffa, M. Nadalini, E. Nardi, E. Borini, A. Borini., and E. Porcu, L. Notarangelo , L. Cipriani, G. Damiano, P.M. Ciotti, N. Calza, S. Zuffa, M. Nadalini, E. Nardi, E. Borini, A. Borini.
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RESVERATROL, HUMAN SPERM, CRYOPRESERVATION, ART - Published
- 2020
121. Chronic mild stress induces anhedonic behavior and changes in glutamate release, BDNF trafficking and dendrite morphology only in stress vulnerable rats. The rapid restorative action of ketamine
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Tiziana Bonifacino, Luca La Via, Daniela Bonini, Paolo Tornese, Maurizio Popoli, Gregers Wegener, Jens R. Nyengaard, Alessandro Ieraci, Jessica Mingardi, Giambattista Bonanno, Stefano Calza, Mara Seguini, Alessandro Barbon, N. Sala, Marco Milanese, Giulia Treccani, Laura Musazzi, 22353003 - Wegener, Gregers, Tornese, P, Sala, N, Bonini, D, Bonifacino, T, La Via, L, Milanese, M, Treccani, G, Seguini, M, Ieraci, A, Mingardi, J, Nyengaard, J, Calza, S, Bonanno, G, Wegener, G, Barbon, A, Popoli, M, and Musazzi, L
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Physiology ,Chronic stre ,Hippocampus ,Dendrite ,Antidepressant ,Biochemistry ,lcsh:RC346-429 ,lcsh:RC321-571 ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Glutamatergic ,0302 clinical medicine ,Endocrinology ,Stress vulnerability ,Chronic stress, Ketamine, Stress vulnerability, Glutamate release, BDNF, Antidepressant ,Medicine ,Chronic stress ,Original Research Article ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Molecular Biology ,lcsh:Neurology. Diseases of the nervous system ,Endocrine and Autonomic Systems ,business.industry ,lcsh:QP351-495 ,Glutamate receptor ,Anhedonia ,030227 psychiatry ,lcsh:Neurophysiology and neuropsychology ,medicine.anatomical_structure ,BDNF ,nervous system ,NMDA receptor ,Ketamine ,Glutamate release ,medicine.symptom ,business ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Depression is a debilitating mental disease, characterized by persistent low mood and anhedonia. Stress represents a major environmental risk factor for depression; the complex interaction of stress with genetic factors results in different individual vulnerability or resilience to the disorder. Dysfunctions of the glutamate system have a primary role in depression. Clinical neuroimaging studies have consistently reported alterations in volume and connectivity of cortico-limbic areas, where glutamate neurons and synapses predominate. This is confirmed by preclinical studies in rodents, showing that repeated stress induces morphological and functional maladaptive changes in the same brain regions altered in humans. Confirming the key role of glutamatergic transmission in depression, compelling evidence has shown that the non-competitive NMDA receptor antagonist, ketamine, induces, at sub-anesthetic dose, rapid and sustained antidepressant response in both humans and rodents.We show here that the Chronic Mild Stress model of depression induces, only in stress-vulnerable rats, depressed-like anhedonic behavior, together with impairment of glutamate/GABA presynaptic release, BDNF mRNA trafficking in dendrites and dendritic morphology in hippocampus. Moreover, we show that a single administration of ketamine restores, in 24 h, normal behavior and most of the cellular/molecular maladaptive changes in vulnerable rats. Interestingly, ketamine treatment did not restore BDNF mRNA levels reduced by chronic stress but rescued dendritic trafficking of BDNF mRNA.The present results are consistent with a mechanism of ketamine involving rapid restoration of synaptic homeostasis, through re-equilibration of glutamate/GABA release and dendritic BDNF for synaptic translation and reversal of synaptic and circuitry impairment. Keywords: Chronic stress, Ketamine, Stress vulnerability, Glutamate release, BDNF, Antidepressant
- Published
- 2019
122. Machine learning in clinical and epidemiological research: Isn't it time for biostatisticians to work on it?
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Marika Vezzoli, Veronica Sciannameo, Dario Gregori, Fabiola Giudici, Valeria Edefonti, Andrea Faragalli, Francesca Ieva, Giulia Barbati, Ilaria Gandin, Giovanni Fiorito, Andrea Ricotti, Danila Azzolina, Alberto Milanese, Pasquale Dolce, Michele Marchioni, Daniele Bottigliengo, Corrado Lanera, Ileana Baldi, Andrea Bucci, Giuliana Solinas, Paola Berchialla, Stefano Calza, Caterina Gregorio, Giulia Lorenzoni, Azzolina, D., Baldi, I., Barbati, G., Berchialla, P., Bottigliengo, D., Bucci, A., Calza, S., Dolce, P., Edefonti, V., Faragalli, A., Fiorito, G., Gandin, I., Giudici, F., Gregori, D., Gregorio, C., Ieva, F., Lanera, C., Lorenzoni, G., Marchioni, M., Milanese, A., Ricotti, A., Sciannameo, V., Solinas, G., and Vezzoli, M.
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medicine.medical_specialty ,education ,Socio-culturale ,Machine learning ,computer.software_genre ,Machine Learning ,Economica ,machine learning, statistics, epidemiology ,Epidemiology ,medicine ,lcsh:R5-920 ,biomedical research ,ComputingMilieux_THECOMPUTINGPROFESSION ,business.industry ,lcsh:Public aspects of medicine ,Ambientale ,lcsh:RA1-1270 ,Medical statistics ,Work (electrical) ,statistics ,epidemiology ,Artificial intelligence ,lcsh:Medicine (General) ,business ,Psychology ,computer - Abstract
In recent years, there has been a widespread cross-fertilization between Medical Statistics and Machine Learning (ML) techniques.
- Published
- 2019
123. FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients
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Ettore Capoluongo, Franco Odicino, Chiara Romani, Maurizio D'Incalci, Laura Zanotti, Concetta Santonocito, Paolo Cappella, Mattia Bugatti, Eugenio Erba, Sergio Pecorelli, Sergio Marchini, Enrico Sartori, Stefano Calza, Moris Cadei, Eric R. Siegel, Laura Tassone, Elisabetta Bandiera, Renata A. Tassi, Luca Beltrame, Carla Donzelli, Antonella Ravaggi, Donatella Guarino, Laura Ardighieri, Paola Todeschini, Alessandro D. Santin, Eliana Bignotti, Tassi, Ra, Todeschini, P, Siegel, Er, Calza, S, Cappella, P, Ardighieri, L, Cadei, M, Bugatti, M, Romani, C, Bandiera, E, Zanotti, L, Tassone, L, Guarino, D, Santonocito, C, Capoluongo, E, Beltrame, L, Erba, E, Marchini, S, D'Incalci, M, Donzelli, C, Santin, Ad, Pecorelli, S, Sartori, E, Bignotti, E, Odicino, F, and Ravaggi, A
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0301 basic medicine ,Cancer Research ,Microarray ,endocrine system diseases ,DNA Repair ,Subtype ,Kaplan-Meier Estimate ,Carcinoma, Ovarian Epithelial ,medicine.disease_cause ,Metastasis ,chemistry.chemical_compound ,Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA ,0302 clinical medicine ,Anticancer drug ,Cell line ,Chemoresistance ,Epithelial ovarian cancer ,FOXM1 ,Immunohistochemistry ,Prognosis ,Oncology ,Cell Movement ,Antineoplastic Combined Chemotherapy Protocols ,Protein Isoforms ,Neoplasms, Glandular and Epithelial ,Neoplasm Metastasis ,RNA, Small Interfering ,Aged, 80 and over ,Ovarian Neoplasms ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,female genital diseases and pregnancy complications ,3. Good health ,Gene Expression Regulation, Neoplastic ,Serous fluid ,Cell Transformation, Neoplastic ,030220 oncology & carcinogenesis ,Gene Knockdown Techniques ,Disease Progression ,Female ,Adult ,Prognosi ,Biology ,lcsh:RC254-282 ,Olaparib ,03 medical and health sciences ,Cell Line, Tumor ,medicine ,Biomarkers, Tumor ,Humans ,Aged ,Cell Proliferation ,Neoplasm Staging ,Oncogene ,Gene Expression Profiling ,Research ,Forkhead Box Protein M1 ,medicine.disease ,Cystadenocarcinoma, Serous ,Gene expression profiling ,030104 developmental biology ,chemistry ,Drug Resistance, Neoplasm ,Cancer research ,Neoplasm Grading ,Carcinogenesis - Abstract
Background Epithelial ovarian cancer (EOC) is a spectrum of different diseases, which makes their treatment a challenge. Forkhead box M1 (FOXM1) is an oncogene aberrantly expressed in many solid cancers including serous EOC, but its role in non-serous EOCs remains undefined. We examined FOXM1 expression and its correlation to prognosis across the three major EOC subtypes, and its role in tumorigenesis and chemo-resistance in vitro. Methods Gene signatures were generated by microarray for 14 clear-cell and 26 endometrioid EOCs, and 15 normal endometrium snap-frozen biopsies. Validation of FOXM1 expression was performed by RT–qPCR and immunohistochemistry in the same samples and additionally in 50 high-grade serous EOCs and in their most adequate normal controls (10 luminal fallopian tube and 20 ovarian surface epithelial brushings). Correlations of FOXM1 expression to clinic-pathological parameters and patients’ prognosis were evaluated by Kaplan-Meier and Cox proportional-hazards analyses. OVCAR-3 and two novel deeply characterized EOC cell lines (EOC-CC1 and OSPC2, with clear-cell and serous subtype, respectively) were employed for in vitro studies. Effects of FOXM1 inhibition by transient siRNA transfection were evaluated on cell-proliferation, cell-cycle, colony formation, invasion, and response to conventional first- and second-line anticancer agents, and to the PARP-inhibitor olaparib. Gene signatures of FOXM1-silenced cell lines were generated by microarray and confirmed by RT-qPCR. Results A significant FOXM1 mRNA up-regulation was found in EOCs compared to normal controls. FOXM1 protein overexpression significantly correlated to serous histology (p = 0.001) and advanced FIGO stage (p = 0.004). Multivariate analyses confirmed FOXM1 protein overexpression as an independent indicator of worse disease specific survival in non-serous EOCs, and of shorter time to progression in platinum-resistant cases. FOXM1 downregulation in EOC cell lines inhibited cell growth and clonogenicity, and promoted the cytotoxic effects of platinum compounds, doxorubicin hydrochloride and olaparib. Upon FOXM1 knock-down in EOC-CC1 and OSPC2 cells, microarray and RT-qPCR analyses revealed the deregulation of several common and other unique subtype-specific FOXM1 putative targets involved in cell cycle, metastasis, DNA repair and drug response. Conclusions FOXM1 is up-regulated in all three major EOCs subtypes, and is a prognostic biomarker and a potential combinatorial therapeutic target in platinum resistant disease, irrespective of tumor histology. Electronic supplementary material The online version of this article (doi:10.1186/s13046-017-0536-y) contains supplementary material, which is available to authorized users.
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- 2017
124. Acute Footshock Stress Induces Time-Dependent Modifications of AMPA/NMDA Protein Expression and AMPA Phosphorylation
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Daniela Bonini, Maurizio Popoli, Alessandro Barbon, Cristina Mora, Paolo Tornese, Luca La Via, N. Sala, Marco Milanese, G. Bonanno, Alice Filippini, Massimo Gennarelli, Giorgio Racagni, Stefano Calza, Laura Musazzi, Bonini, D, Mora, C, Tornese, P, Sala, N, Filippini, A, La Via, L, Milanese, M, Calza, S, Bonanno, G, Racagni, G, Gennarelli, M, Popoli, M, Musazzi, L, and Barbon, A
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0301 basic medicine ,Male ,Time Factors ,Article Subject ,glutamate receptor ,molecular mechanisms ,AMPA receptor ,Neurology ,Neurology (clinical) ,Biology ,Receptors, N-Methyl-D-Aspartate ,lcsh:RC321-571 ,Rats, Sprague-Dawley ,03 medical and health sciences ,Glutamatergic ,0302 clinical medicine ,Postsynaptic potential ,Animals ,RNA, Messenger ,Receptors, AMPA ,Phosphorylation ,Long-term depression ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Electroshock ,Glutamate receptor ,Brain ,acute stre ,Cell biology ,Rats ,Protein Subunits ,030104 developmental biology ,Silent synapse ,Synapses ,NMDA receptor ,Corticosterone ,Neuroscience ,030217 neurology & neurosurgery ,Stress, Psychological ,Ionotropic effect ,Research Article - Abstract
Clinical studies on patients with stress-related neuropsychiatric disorders reported functional and morphological changes in brain areas where glutamatergic transmission is predominant, including frontal and prefrontal areas. In line with this evidence, several preclinical works suggest that glutamate receptors are targets of both rapid and long-lasting effects of stress. Here we found that acute footshock- (FS-) stress, although inducing no transcriptional and RNA editing alterations of ionotropic AMPA and NMDA glutamate receptor subunits, rapidly and transiently modulates their protein expression, phosphorylation, and localization at postsynaptic spines in prefrontal and frontal cortex. In total extract, FS-stress increased the phosphorylation levels of GluA1 AMPA subunit at Ser845immediately after stress and of GluA2 Ser8802 h after start of stress. At postsynaptic spines, stress induced a rapid decrease of GluA2 expression, together with an increase of its phosphorylation at Ser880, suggesting internalization of GluA2 AMPA containing receptors. GluN1 and GluN2A NMDA receptor subunits were found markedly upregulated in postsynaptic spines, 2 h after start of stress. These results suggest selected time-dependent changes in glutamatergic receptor subunits induced by acute stress, which may suggest early and transient enhancement of AMPA-mediated currents, followed by a transient activation of NMDA receptors.
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- 2016
125. Nutrient-based dietary patterns and nasopharyngeal cancer: Evidence from an exploratory factor analysis
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Valeria Edefonti, Francesca Bravi, Adriano Decarli, Werner Garavello, Ettore Bidoli, Cristina Bosetti, Monica Ferraroni, Diego Serraino, C. La Vecchia, Jerry Polesel, Stefano Calza, Federica Nicolussi, Edefonti, V, Nicolussi, F, Polesel, J, Bravi, F, Bosetti, C, Garavello, W, La Vecchia, C, Bidoli, E, Decarli, A, Serraino, D, Calza, S, and Ferraroni, M
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Adult ,Dietary Fiber ,Male ,Pathology ,medicine.medical_specialty ,Cancer Research ,Adolescent ,dietary patterns ,Nasopharyngeal neoplasm ,nasopharyngeal cancer ,factor analysis ,Physiology ,Vegetable ,Logistic regression ,Settore MED/01 - STATISTICA MEDICA ,Young Adult ,nutrients ,Vegetables ,Humans ,Medicine ,Young adult ,Aged ,Animal fat ,Nasopharyngeal Neoplasm ,business.industry ,nutrient ,Case-control study ,Cancer ,Nasopharyngeal Neoplasms ,Nutrition Survey ,Odds ratio ,Feeding Behavior ,Middle Aged ,Nutrition Surveys ,medicine.disease ,Diet ,Quartile ,factor analysi ,Italy ,Oncology ,Case-Control Studies ,Clinical Study ,Carcinoma, Squamous Cell ,Female ,dietary pattern ,business ,Case-Control Studie ,Human - Abstract
Background: The issue of diet and laryngeal cancer has been rarely addressed considering the potential role of dietary patterns. Methods: We examined this association using data from a case-control study carried out between 1992 and 2000. Cases were 460 histologically confirmed incident laryngeal cancers hospitalized in two Italian areas. Controls were 1,088 subjects hospitalized for acute nonneoplastic diseases unrelated to tobacco or alcohol consumption. Dietary habits were investigated through a 78-item food frequency questionnaire. A posteriori dietary patterns were identified through principal component factor analysis carried out on a selected set of 28 major nutrients. The internal reproducibility, robustness, and reliability of the identified patterns were evaluated. Odds ratios (OR) of laryngeal cancer and 95% confidence intervals (95% CI) were estimated using unconditional multiple logistic regression models on quartiles of factor scores. Results: We identified five major dietary patterns named “animal products, ”“ starch-rich, ”“ vitamins and fiber, ”“ vegetable unsaturated fatty acids,” and “animal unsaturated fatty acids.” The vitamins and fiber dietary pattern was inversely associated with laryngeal cancer (OR, 0.35; 95% CI, 0.24-0.52 for the highest versus the lowest score quartile), whereas the animal products (OR, 2.34; 95% CI, 1.59-3.45) and the animal unsaturated fatty acids (OR, 2.07; 95% CI, 1.42-3.01) patterns were directly associated with it. There was no significant association between the vegetable unsaturated fatty acids and the starch-rich patterns and laryngeal cancer risk. Conclusion: These findings suggest that diets rich in animal products and animal fats are directly related, and those rich in fruit and vegetables inversely related, to laryngeal cancer risk. Cancer Epidemiol Biomarkers
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- 2015
126. Fetal DNA detection in maternal plasma throughout gestation
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Augusto Ferrari, Laura Cremonesi, Elsa Viora, Gabriella Restagno, S. Bastonero, Marco Pagliano, Maddalena Smid, Dania Gambini, Maurizio Ferrari, Silvia Galbiati, M. Campogrande, Stefano Calza, Galbiati, S, Smid, M, Gambini, D, Ferrari, A, Restagno, G, Viora, E, Campogrande, M, Bastonero, S, Pagliano, M, Calza, S, Ferrari, Maurizio, and Cremonesi, L.
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Male ,Sex Determination Analysis ,medicine.medical_specialty ,Gestational Age ,Prenatal diagnosis ,Biology ,Cohort Studies ,Fetus ,Pregnancy ,Prenatal Diagnosis ,Genetics ,medicine ,Humans ,Genes, sry ,Maternal-Fetal Exchange ,Genetics (clinical) ,Genetic testing ,medicine.diagnostic_test ,Obstetrics ,Gestational age ,DNA ,medicine.disease ,Testis determining factor ,Cell-free fetal DNA ,Gestation ,Female ,Pregnancy Trimesters - Abstract
The presence of fetal DNA in maternal plasma may represent a source of genetic material which can be obtained noninvasively. We wanted to assess whether fetal DNA is detectable in all pregnant women, to define the range and distribution of fetal DNA concentration at different gestational ages, to identify the optimal period to obtain a maternal blood sample yielding an adequate amount of fetal DNA for prenatal diagnosis, and to evaluate accuracy and predictive values of this approach. This information is crucial to develop safe and reliable non-invasive genetic testing in early pregnancy and monitoring of pregnancy complications in late gestation. Fetal DNA quantification in maternal plasma was carried out by real-time PCR on the SRY gene in male-bearing pregnancies to distinguish between maternal and fetal DNA. A cohort of 1,837 pregnant women was investigated. Fetal DNA could be detected from the sixth week and could be retrieved at any gestational week. No false-positive results were obtained in 163 women with previous embryo loss or previous male babies. Fetal DNA analysis performed blindly on a subset of 464 women displayed 99.4, 97.8 and 100% accuracy in fetal gender determination during the first, second, and third trimester of pregnancy, respectively. No SRY amplification was obtained in seven out of the 246 (2.8%) male-bearing pregnancies. Fetal DNA from maternal plasma seems to be an adequate and reliable source of genetic material for a noninvasive prenatal diagnostic approach.
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- 2005
127. Dietary fibres and ovarian cancer risk
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Silvia Franceschi, E. Negri, Liliane Chatenoud, Claudio Pelucchi, L. Dal Maso, E. Conti, Stefano Calza, Maurizio Montella, C. La Vecchia, Pelucchi C, La Vecchia C, Chatenoud L, Negri E, Conti E, Montella M, Calza S, Dal Maso L, and Franceschi S
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Adult ,Dietary Fiber ,Cancer Research ,medicine.medical_specialty ,Percentile ,Logistic regression ,Age Distribution ,Neoplastic Syndromes, Hereditary ,Internal medicine ,Vegetables ,Epidemiology ,Odds Ratio ,medicine ,Humans ,Family history ,Risk factor ,Aged ,Ovarian Neoplasms ,Gynecology ,business.industry ,Odds ratio ,Middle Aged ,medicine.disease ,Confidence interval ,Parity ,Logistic Models ,Oncology ,Case-Control Studies ,Educational Status ,Female ,Menopause ,Ovarian cancer ,business - Abstract
Data from an Italian multicentre case-control study on ovarian cancer were used to analyse the relationship between various types of fibres and ovarian cancer risk. The study, conducted between 1992 and 1999, included 1031 cases of incident, histologically-confirmed epithelial ovarian cancer. Controls were 2411 women admitted to the same network of hospitals for acute, non-malignant, non-hormonal-related diseases. Cases and controls were interviewed using a validated food frequency questionnaire (FFQ). Odds ratios (ORs), and the corresponding 95% confidence intervals (CI), were estimated using unconditional multiple logistic regression models. For total (Englyst) fibre, the OR for the highest versus the lowest quintile of intake was 0.68, and the continuous OR for the difference between the 80th and the 20th percentile of intake was 0.87. For most types of fibre, the continuous OR was significantly below 1. The OR was 0.83 for cellulose, 0.89 for soluble non-cellulose polysaccharides (NCPs), 0.86 for total insoluble fibre, 0.92 for insoluble NCP, and 0.95 (non-significant) for lignin. The inverse association was consistent across strata of age, family history and menopausal status, even if the association was apparently stronger in postmenopausal women. When fibre was lassified according to the source, vegetable (but not grain) fibres, showed a significant protective effect, with an OR of 0.78. (C) 2001 Elsevier Science Ltd. All rights reserved. RI Calza, Stefano/B-1915-2010
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- 2001
128. mRNA Expression Signature of Gleason Grade Predicts Lethal Prostate Cancer
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Michelangelo Fiorentino, Massimo Loda, Katja Fall, Swen Olof Andersson, Stephen P. Finn, Lorelei A. Mucci, Richard Flavin, Mark A. Rubin, Neil E. Martin, Jan-Erik Johansson, Jennifer R. Stark, Todd R. Golub, Hans-Olov Adami, Meir J. Stampfer, Sunita R. Setlur, Kathryn L. Penney, Sven Perner, Peter Kraft, Ove Andrén, Howard D. Sesso, Jennifer A. Sinnott, Matthew L. Freedman, Stefano Calza, Yujin Hoshida, Yudi Pawitan, Philip W. Kantoff, Penney KL, Sinnott JA, Fall K, Pawitan Y, Hoshida Y, Kraft P, Stark JR, Fiorentino M, Perner S, Finn S, Calza S, Flavin R, Freedman ML, Setlur S, Sesso HD, Andersson SO, Martin N, Kantoff PW, Johansson JE, Adami HO, Rubin MA, Loda M, Golub TR, Andrén O, Stampfer MJ, and Mucci LA
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Male ,Oncology ,Cancer Research ,Pathology ,medicine.medical_specialty ,medicine.medical_treatment ,Disease ,Gleason grade ,urologic and male genital diseases ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Prostate ,Internal medicine ,Original Reports ,medicine ,Humans ,neoplasms ,Aged ,030304 developmental biology ,Aged, 80 and over ,0303 health sciences ,prostate cancer, gene expression, lethality ,business.industry ,Gene Expression Profiling ,Area under the curve ,Prostatic Neoplasms ,Cell Differentiation ,Middle Aged ,medicine.disease ,3. Good health ,Gene expression profiling ,medicine.anatomical_structure ,surgical procedures, operative ,030220 oncology & carcinogenesis ,Cohort ,Disease Progression ,business ,Watchful waiting - Abstract
Purpose Prostate-specific antigen screening has led to enormous overtreatment of prostate cancer because of the inability to distinguish potentially lethal disease at diagnosis. We reasoned that by identifying an mRNA signature of Gleason grade, the best predictor of prognosis, we could improve prediction of lethal disease among men with moderate Gleason 7 tumors, the most common grade, and the most indeterminate in terms of prognosis. Patients and Methods Using the complementary DNA–mediated annealing, selection, extension, and ligation assay, we measured the mRNA expression of 6,100 genes in prostate tumor tissue in the Swedish Watchful Waiting cohort (n = 358) and Physicians' Health Study (PHS; n = 109). We developed an mRNA signature of Gleason grade comparing individuals with Gleason ≤ 6 to those with Gleason ≥ 8 tumors and applied the model among patients with Gleason 7 to discriminate lethal cases. Results We built a 157-gene signature using the Swedish data that predicted Gleason with low misclassification (area under the curve [AUC] = 0.91); when this signature was tested in the PHS, the discriminatory ability remained high (AUC = 0.94). In men with Gleason 7 tumors, who were excluded from the model building, the signature significantly improved the prediction of lethal disease beyond knowing whether the Gleason score was 4 + 3 or 3 + 4 (P = .006). Conclusion Our expression signature and the genes identified may improve our understanding of the de-differentiation process of prostate tumors. Additionally, the signature may have clinical applications among men with Gleason 7, by further estimating their risk of lethal prostate cancer and thereby guiding therapy decisions to improve outcomes and reduce overtreatment.
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- 2011
129. Evaluation of a panel of circulating DNA, RNA and protein potential markers for pathologies of pregnancy
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Paolo Cavoretto, Silvia M.A. Pedroni, Maria Teresa Castiglioni, Salvianti Francesca, Pamela Pinzani, Silvia Galbiati, Laura Cremonesi, Stefano Calza, Maddalena Smid, Patrizia Rovere-Querini, Claudio Orlando, Maurizio Ferrari, Federica Pasi, Vincenza Causarano, Galbiati, S, Causarano, V, Pinzani, P, Francesca, S, Orlando, C, Smid, M, Pasi, F, Castiglioni, Mt, Cavoretto, P, ROVERE QUERINI, Patrizia, Pedroni, S, Calza, S, Ferrari, Maurizio, and Cremonesi, L.
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medicine.medical_specialty ,Corticotropin-Releasing Hormone ,Clinical Biochemistry ,Biology ,Preeclampsia ,Pre-Eclampsia ,Pregnancy ,Internal medicine ,Blood plasma ,medicine ,Humans ,RNA, Messenger ,Messenger RNA ,Fetus ,Fetal Growth Retardation ,Biochemistry (medical) ,Gestational age ,General Medicine ,PTX3 ,DNA ,medicine.disease ,Serum Amyloid P-Component ,Endocrinology ,C-Reactive Protein ,Gestation ,Female ,Biomarkers - Abstract
Background: Among markers of pregnancy complications, corticotropin-releasing hormone (CRH) mRNA, long pentraxin 3 (PTX3) protein and fetal and total DNA had been reported to be increased in the plasma of women with overt preeclampsia (PE). We developed an optimized protocol to evaluate whether concentrations of CRH mRNA, PTX3 mRNA and protein, fetal and/or total DNA are increased in fetal growth restriction (FGR), and whether they predict complications of pregnancy. Methods: The protocol included a preamplification step to enrich rare mRNA species. CRH and PTX3 mRNA, DNA and PTX3 protein were measured in the plasma of women with PE or FGR, in women at risk of developing these pathologies and in healthy women matched for gestational age. Results: CRH mRNA, fetal and/or total DNA and PTX3 protein were significantly increased in women with overt PE when compared to controls. Pregnant women who later developed PE or FGR during pregnancy showed total DNA levels that were significantly increased before the onset of both pathologies, while RNA markers were increased only in women who later developed PE. Conclusions: Our protocol for plasma RNA quantification may allow for the extension of a panel of predictive markers to be investigated in larger patient cohorts. Clin Chem Lab Med 2010;48:791–4.
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- 2010
130. Cryotherapy in the prevention of oral mucositis in patients receiving low-dose methotrexate following myeloablative allogeneic stem cell transplantation: a prospective randomized study of the Gruppo Italiano Trapianto di Midollo Osseo nurses group
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Giuseppe Bandini, A Errico, M M Banfi, E Polato, F Alberani, Mario Arpinati, M C Altieri, Alberto Bosi, V Sabbi, Michele Baccarani, M C Loddo, M Berni, G Costazza, E Pirola, Francesca Bonifazi, A Mega, L Barzetti, E Gori, S Leanza, A. De Vivo, C Brignoli, C Feraut, C Borrelli, S Calza, Gori E, Arpinati M, Bonifazi F, Errico A, Mega A, Alberani F, Sabbi V, Costazza G, Leanza S, Borrelli C, Berni M, Feraut C, Polato E, Altieri MC, Pirola E, Loddo MC, Banfi M, Barzetti L, Calza S, Brignoli C, Bandini G, De Vivo A, Bosi A, and Baccarani M.
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,medicine.drug_class ,medicine.medical_treatment ,Cryotherapy ,Antineoplastic Agents ,Hematopoietic stem cell transplantation ,Antimetabolite ,law.invention ,Folinic acid ,Randomized controlled trial ,law ,medicine ,Mucositis ,Humans ,Transplantation, Homologous ,Child ,Stomatitis ,Transplantation ,business.industry ,Hematopoietic Stem Cell Transplantation ,Hematology ,Middle Aged ,medicine.disease ,Surgery ,Methotrexate ,Female ,business ,medicine.drug - Abstract
Severe oral mucositis is a major cause of morbidity following allogeneic hematopoietic stem cell transplantation (AHSCT). Cryotherapy, that is, the application of ice chips on the mucosa of the oral cavity during the administration of antineoplastic agents, may reduce the incidence and severity of chemotherapy-related oral mucositis. In this multicenter randomized study, we addressed whether cryotherapy during MTX administration is effective in the prevention of severe oral mucositis in patients undergoing myeloablative AHSCT. One hundred and thirty patients undergoing myeloablative AHSCT and MTX-containing GVHD prophylaxis were enrolled and randomized to receive or not receive cryotherapy during MTX administration. The incidence of severe (grade 3-4) oral mucositis, the primary end point of the study, was comparable in patients receiving or not cryotherapy. Moreover, no difference was observed in the incidence of oral mucositis grade 2-4 and the duration of oral mucositis grade 3-4 or 2-4, or in the kinetics of mucositis over time. In univariate and multivariate analysis, severe oral mucositis correlated with TBI in the conditioning regimen and lack of folinic acid rescue following MTX administration. Thus, cryotherapy during MTX administration does not reduce severe oral mucositis in patients undergoing myeloablative allogeneic HSCT. Future studies will assess cryotherapy before allogeneic HSCT.
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- 2007
131. Low brain-derived neurotrophic factor (BDNF) levels in serum of Huntington's disease patients
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Barbara Poletti, Stefano Calza, Vincenzo Silani, Ferdinando Squitieri, Milena Cannella, Luigi Frati, Jenny Sassone, Andrea Ciammola, Ciammola, A, SASSONE PAGANO, Jenny, Cannella, M, Calza, S, Poletti, B, Frati, L, Squitieri, F, and Silani, V.
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bdnf ,human serum ,huntington's disease ,Adult ,Male ,medicine.medical_specialty ,Huntingtin ,Disease ,Central nervous system disease ,Cellular and Molecular Neuroscience ,Degenerative disease ,Huntington's disease ,Neurotrophic factors ,Internal medicine ,Basal ganglia ,medicine ,Humans ,Genetics (clinical) ,Aged ,Brain-derived neurotrophic factor ,business.industry ,Brain-Derived Neurotrophic Factor ,Middle Aged ,medicine.disease ,Psychiatry and Mental health ,Huntington Disease ,Endocrinology ,nervous system ,Case-Control Studies ,Regression Analysis ,Female ,business - Abstract
Huntington's disease (HD) is a neurodegenerative disorder characterized by motor, cognitive, and psychiatric symptoms and by a progressive degeneration of neurons in basal ganglia and in brain cortex. Brain-derived neurotrophic factor (BDNF) is a pro-survival factor for striatal neurons. Some evidence implicates a brain BDNF deficiency, related to mutated huntingtin expression, in the selective vulnerability of striatal neurons in HD. We compared BDNF serum levels in 42 patients with HD (range 28–72 years, mean age 51.9 ± 11.5), and 42 age-matched healthy subjects (range 25–68 years, mean age 48.2 ± 12.5). We evaluated the potential relationship between BDNF serum levels, CAG repeat number (range 40–54, mean 44.8 ± 3.4) and duration of illness (range 6–228 months, mean 103.6 ± 62.1). Serum BDNF levels were significantly lower in patients than in age-matched healthy subjects. Lower BDNF levels were associated with a longer CAG repeat length and a longer duration of illness. Severity of the illness, as assessed by the Unified Huntington's Disease Rating Scale (UHDRS) motor and cognitive scores, was negatively related to serum BDNF levels. These results in vivo confirm that the huntingtin mutation causes BDNF production to decline and show that the BDNF deficiency is detectable in HD patients' sera. Further studies on a larger sample size should confirm whether BDNF concentrations in patients' serum could be a useful clinical marker related to the patients' disease phenotype. © 2007 Wiley-Liss, Inc.
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- 2007
132. Offspring sexual dimorphism and sex-allocation in relation to parental age and paternal ornamentation in the barn swallow
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Roberta Martinelli, Roberto Ambrosini, Stefano Calza, Andrea Pilastro, Ap Moller, Nicola Saino, Saino, N, Ambrosini, R, Martinelli, R, Calza, S, Møller, A, and Pilastro, A
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Male ,Sexual characteristics ,Sex Characteristics ,Offspring ,cost of sons and daughters, Hirundo rustica, laying order, parental age, secondary sexual characters, sex ratio ,Age Factors ,Zoology ,Sexing ,Biology ,Biological Evolution ,Sexual dimorphism ,Songbirds ,Survival Rate ,Sexual Behavior, Animal ,Sexual selection ,Genetics ,Animals ,Female ,BIO/07 - ECOLOGIA ,Reproductive value ,Ecology, Evolution, Behavior and Systematics ,Sex allocation ,Sex ratio ,BIO/05 - ZOOLOGIA - Abstract
We analysed the morphology of nestling barn swallows ( Hirundo rustica ) in relation to their sex, and laying and hatching order. In addition, we studied sex-allocation in relation to parentage, parental age and expression of a secondary sexual character of fathers. Molecular sexing was conducted using the sex chromosome-linked avian CHD1 gene. Sex of the offspring was not associated with laying or hatching order. None of nine morphological, serological and immunological variables varied in relation to offspring sex. Sexual dimorphism did not vary in relation to parental age and expression of a paternal secondary sexual character. The proportion of sons declined with brood size. Individual males and females had a similar proportion of sons during consecutive breeding years. The proportion of sons of individual females declined with age, but increased with the expression of a secondary sexual character of their current mate. The generalized lack of variation in sexual dimorphism among nestlings may suggest that barn swallows do not differentially invest in sons vs. daughters. Alternatively, male offspring may require different parental effort compared to their female siblings in order to attain the same morphological state. The lack of variation in offspring sexual dimorphism with paternal ornamentation suggests no adjustment of overall parental effort in relation to reproductive value of the two sexes. However, malebiased sex ratio among offspring of highly ornamented males may represent an adaptive sex-allocation strategy because the expression of male ornaments is heritable and highly ornamented males are at a sexual selection advantage.
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- 2002
133. microRNAs as new biomarkers in frailty associated with cognitive impairment in elderly patients (MATCH-In project)
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Giulia Carini, Jessica Mingardi, Luca La Via, Francesco, Bolzetta, Annalisa, Tessari, Alberto, Cester, Stefania, Maggi, Veronese, Nicola, Stefano Calza, Leonardo Elia, Fiorentini, Chiara, Laura, Musazzi, Alessandro, Ieraci, Maurizio, Popoli, Barbon, Alessandro, Carini, G, Mingardi, J, La Via, L, Bolzetta, F, Tessari, A, Cester, A, Maggi, S, Veronese, N, Calza, S, Elia, L, Fiorentini, C, Musazzi, L, Ieraci, A, Popoli, M, and Barbon, A
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microRNA, frailty syndrome
134. Guided versus freehand single implant placement: A 3-year parallel randomized clinical trial.
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Lops D, Palazzolo A, Calza S, Proietto L, Sordillo A, Mensi M, and Romeo E
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- Humans, Male, Female, Middle Aged, Adult, Surgery, Computer-Assisted methods, Follow-Up Studies, Crowns, Aged, Dental Prosthesis, Implant-Supported, Treatment Outcome, Clinical Competence, Dental Implantation, Endosseous methods, Dental Implants, Single-Tooth, Alveolar Bone Loss diagnostic imaging
- Abstract
Objectives: The present parallel randomized clinical trial aimed to assess, after a 3-year follow-up period, whether the choice of surgical technique-either manual or guided-and of the operator - non-expert operator or skilled - can affect the stability of peri‑implant marginal bone levels in implants placed 1 mm sub-crestal., Materials and Methods: Patients received platform-switched implants (Anyridge, MegaGen Implant Co., Gyeongbuk, South Korea) featuring a 5-degree internal conical connection and supporting single screw-retained fixed crowns. The implants were randomly assigned to be placed through a digitally static guided surgery procedure (Test group - GS) or a freehand surgical technique (Control Group - FH). A non-expert operator (fewer than 20 implants placed in his professional activity) was selected to perform procedures for the GS Group, while a skilled operator (with over 1000 implants placed in his professional activity) was chosen for the FH Group. Marginal bone level (MBL) was measured at prosthesis installation (t0) and at 1 (t1), 2 (t2) and 3 years (t3) of follow-up. Changes in MBL from t0 to t3 were analyzed through periapical radiographs. Moreover, MBL changes at all time points were correlated to different supra-crestal soft tissue heights (STH): less than 3 and ≥ 3 mm, respectively., Results: 60 implants in 18 patients were examined, with 30 implants allocated to the GS group and 30 to the FH group. The difference in MBL change between the two groups was 0.11 ± 0.22 mm, which was not statistically significant (p = 0.61). At the time of prosthetic loading, the mean MBL for implants with STH less than 3 mm was 0.33 mm higher than implants with STH ≥ 3 mm, though this difference was not statistically significant (P = 0.065)., Conclusions: Digitally static guided implant placement, performed by a non-expert operator, does not limit marginal bone remodeling, when compared to a freehand procedure performed by an experienced operator., Clinical Significance: After correct and careful planning, early marginal bone levels (MBL) around conical connection, platform-switched implants placed sub-crestally may be stable in time. Digital planning and surgery have the potential to assist non-expert clinicians in achieving implant placements with comparable outcomes to those performed by experts., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
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- 2024
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135. Natural history of cerebral visual impairment in children with cerebral palsy.
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Galli J, Loi E, Calza S, Micheletti S, Molinaro A, Franzoni A, Rossi A, Semeraro F, Merabet LB, and Fazzi E
- Abstract
Aim: To longitudinally evaluate the natural history of cerebral visual impairment (CVI) in children with cerebral palsy (CP) and identify which early visual signs or symptoms are associated with cognitive visual disorders (CVDs) at school age., Method: Fifty-one individuals with CP and CVI underwent an ophthalmological, oculomotor, and basic visual function evaluation at three time points: T0 (6-35 months old); T1 (3-5 years old); and T2 (≥6 years old). We also performed a cognitive visual evaluation at T2. Logistic regression fitted using a generalized estimation equation (binary) and cumulative link models (ordinal) were used to model the outcomes of interest., Results: Ophthalmological deficits were stable over time, except for ocular fundus abnormalities (T1-T0, p = 0.01; T2-T1, p = 0.02; T2-T0, p < 0.01) and strabismus, whose frequency increased with age (T2-T0, p= 0.02 with T2-T0, p = 0.05). Conversely, fixation (T1-T0, T2-T0, p < 0.01), smooth pursuit (T2-T1, T2-T0, p < 0.01), saccades (T1-T0, T2-T1, T2-T0, p < 0.01), as well as visual acuity, contrast sensitivity, and visual field (T1-T0, T2-T0, p < 0.01) all improved over time. Early oculomotor dysfunction was associated with CVD at T2., Interpretation: Although a diagnosis of CVI was confirmed in all children at each time point, several visual signs and symptoms improved over time; in some cases, they reached complete recovery at T1 and T2. These results emphasize the 'permanent' but 'not unchanging' nature of the CVI associated with CP during development., (© 2024 The Author(s). Developmental Medicine & Child Neurology published by John Wiley & Sons Ltd on behalf of Mac Keith Press.)
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- 2024
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136. Venetoclax plus decitabine as a bridge to allogeneic haematopoietic stem-cell transplantation in older patients with acute myeloid leukaemia (VEN-DEC GITMO): final report of a multicentre, single-arm, phase 2 trial.
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Russo D, Polverelli N, Bernardi S, Santarone S, Farina M, Borlenghi E, Onida F, Castagna L, Bramanti S, Carella AM, Sorasio R, Martino M, Alati C, Olivieri A, Beltrami G, Curti A, Vetro C, Leotta S, Mancini V, Terruzzi E, Bernardi M, Galieni P, Musto P, Cerretti R, Giaccone L, Skert C, Radici V, Vezzoli M, Calza S, Leoni A, Garuffo L, Bonvicini C, Pellizzeri S, Malagola M, and Ciceri F
- Abstract
Background: Access to allogeneic haematopoietic stem-cell transplantation (HSCT) remains challenging for older patients (aged >60 years) with acute myeloid leukaemia. We aimed to evaluate the efficacy of venetoclax plus decitabine as first-line therapy and bridge to transplantation in this patient population., Methods: This multicentre, single-arm, phase 2 trial was conducted in 20 Gruppo Italiano Trapianto Midollo Osseo (GITMO) centres in Italy. Patients aged ≥60 and <75 years, with newly diagnosed acute myeloid leukaemia categorised as intermediate or high risk according to 2016 WHO and 2017 European LeukemiaNet, an ECOG performance status of less than 2, and considered fit for allogeneic HSCT were included. Patients received oral venetoclax with a 3-day ramp-up: 100 mg on day 1, 200 mg on day 2, and 400 mg once per day from day 3 of cycle one, and then every 28 days of each cycle (two to four in total). Decitabine was administered intravenously at a dose of 20 mg/m
2 from days 1 to 5 every 28 days. At cycle one, patients were admitted to hospital for a minimum of 24 h, whereas subsequent cycles could be administered on an outpatient basis. Two additional cycles were allowed while waiting for allogeneic HSCT or for those with no response or partial response after cycle two. The primary endpoint was the proportion of patients who had allogeneic HSCT performed during first complete remission, assessed in all patients who received at least one dose of the study medication. This study was registered with ClinicalTrials.gov (NCT04476199, ongoing) and EudraCT (2020-002297-26)., Findings: Between June 1, 2021, and Dec 30, 2022, 93 patients were enrolled and started venetoclax plus decitabine induction (44 [47%] at intermediate risk and 49 [53%] at high risk). The median age was 68·5 (IQR 60·3-74·7). All 93 participants were White, of whom 43 (46%) were female and 50 (54%) were male. The median follow-up was 236 days (IQR 121-506). 64 (69%) of 93 patients reached complete remission and 53 (57%) underwent allogeneic HSCT in complete remission. 53 (83%) of 64 with a complete remission underwent allogeneic HSCT. Five (8%) of 64 patients in complete remission relapsed before transplantation and four died as a consequence. Adverse events (grade ≥3) occurred in 49 (53%) of 93 patients. The most common adverse events were infections (including pneumonia, bacterial sepsis, and SARS-CoV-2 causing seven deaths among 28 [57%] of 49 patients), neutropenia (17 [35%]), thrombocytopenia (two [4%], including one fatal CNS bleeding), and cardiac events (four [8%], including one fatal heart failure). No treatment-related deaths were observed., Interpretation: Venetoclax plus decitabine induction can significantly enhance the feasibility of allogeneic HSCT in older patients with acute myeloid leukaemia who are deemed fit for transplantation., Funding: AbbVie and Johnson & Johnson., Competing Interests: Declaration of interests DR received consulting fees from Medac; honoraria and travel grants from Jazz Pharma, Novartis, MSD, Kite Gilead, and Medac; and has participated in data safety monitoring and advisory board meetings for Novartis, MSD, Kite Gilead, Medac, AbbVie, and Janssen. EB received consulting fees from AbbVie; travel grants from Amgen, Novartis, and AbbVie; and has participated in data safety monitoring and advisory board meetings for Otsuka and Amgen. FO received honoraria from Takeda, Medac, and Kyowa; and travel grants from Roche, Janssen, Kyowa, Takeda, Jazz Pharma, and Medac. RS received a travel grant from Takeda. MaM received honoraria from Novartis, MSD, Astellas Pharma, AbbVie, Takeda, Pfizer, Sanofi, Jazz Pharma, Janssen Cilag, Kite Gilead, Medac, and Bristol Myers Squibb; travel grants from Kite Gilead, Roche, and Janssen Cilag; has participated in data safety monitoring and advisory board meetings for Novartis, Takeda, Kite Gilead, Pfizer, Bristol Myers Squibb, and Janssen Cilag; and served as president of GITMO. CA received honoraria from AbbVie and Jazz Pharma; and has participated in data safety monitoring and advisory board meetings for AbbVie, Jazz Pharma, and Amgen. GB received travel grants from Janssen Cilag and Amgen. AC received honoraria from AbbVie, Menarini (Stemline), Pfizer, Jazz Pharma, and Servier; a travel grant from Jazz Pharma; and has participated in data safety monitoring and advisory board meetings for AbbVie and Menarini (Stemline). CG received consulting fees from AbbVie, Jazz Pharma, Incyte, Bristol Myers Squibb, and Astellas; and a travel grant from Jazz Pharma. PM received honoraria and travel grants from AbbVie and Johnson & Johnson. LG received honoraria from Novartis, MSD, Gilead, AbbVie, Sanofi, and Bristol Myers Squibb; and travel grants from Janssen and AbbVie. CS received honoraria from Jazz Pharma and Kite Pharma; and a travel grant from Jazz Pharma. VR received travel grants from Neovii, Medac, and Jazz Pharma. All other authors declare no competing interests., (Copyright © 2024 The Author(s). Published by Elsevier Ltd. All rights reserved, including those for text and data mining, AI training, and similar technologies.)- Published
- 2024
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137. Association of Weight Status and Waist Circumference with Physical Activity in people with Schizophrenia Spectrum Disorders and healthy controls.
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Martinelli A, Leone S, Zamparini M, Carnevale M, Caterson ID, Fuller NR, Calza S, and de Girolamo G
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Background: Individuals with Schizophrenia Spectrum Disorders (SSD) often suffer from obesity and do limited Physical Activity (PA). PA has many beneficial effects on a variety of somatic and mental variables and it should be strengthened among people with mental disorders. The relationship between Body Mass Index (BMI), Waist Circumference (WC), and PA in this population is poorly understood, with a lack of precise PA assessment. This study investigates the association between BMI, WC, weight, and PA in individuals with SSD and controls using accelerometers., Methods: One hundred twenty-six patients with SSD (residents and outpatients) and 110 sex- and age-matched controls were enrolled. Clinical, sociodemographic, and quality-of-life data were collected. PA was measured with a tri-axial ActiGraph GT9X and quantified by Vector Magnitude (VM). Relationships between PA and BMI, WC, and weight changes were analysed using linear regression models., Results: Patients were more likely to be unmarried, unemployed, and less educated compared to controls (p < 0.001). Residents had more medical comorbidities (p = 0.001), while outpatients had higher BMI, weight, and WC (p < 0.001). Residents reported more severe psychopathology, lower functioning, and greater use of psychopharmacological medications (p < 0.001). Higher PA levels were not significantly associated with lower BMI, WC, or weight. Although not statistically significant, increased PA showed a trend towards lower obesity risk., Conclusions: Sociodemographic, medical, and clinical characteristics of individuals with SSD define vulnerability factors that can inform tailored interventions to improve PA., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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138. Xanthan-based chlorhexidine gel effects in non-surgical periodontal therapy? A meta-analysis.
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Mensi M, Palazzolo A, Garzetti G, Lops D, Calza S, and Rota M
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- Humans, Anti-Infective Agents, Local therapeutic use, Randomized Controlled Trials as Topic, Combined Modality Therapy, Chlorhexidine therapeutic use, Polysaccharides, Bacterial therapeutic use, Gels, Dental Scaling, Root Planing
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Objective: To carry out a systematic review and meta-analysis of randomized controlled clinical trials (RCTs) and controlled clinical trials (CCTs) comparing scaling and root planing (SRP) or placebo with subgingival application of xanthan-based CHX (chlorhexidine) gel as adjunct to SRP., Materials and Methods: The literature search was carried out in PubMed/MEDLINE, EMBASE, and SCOPUS; primary outcomes were probing pocket depth (PPD) reduction and gain in clinical attachment level (CAL)., Results: Overall, 15 studies were included. Three studies were judged to be at moderate risk of bias while the remaining 12 were rated at high risk of bias. A significant improvement in PPD reduction (standardized mean difference, SMD, 0.87, 95% CI, 0.41-1.34) and CAL gain (SMD = 0.84, 95% CI, 0.36-1.33) emerged for the SRP + CXH gel compared to the SRP alone group, in the presence of significant high heterogeneity among the studies., Conclusions: Our systematic review and meta-analysis showed that xanthan-based chlorhexidine gel as adjunct to non-surgical periodontal therapy gives benefit in terms of PPD reduction and CAL gain as compared to non-surgical periodontal therapy only. Since there was high heterogeneity among studies and the quality of the evidence is low, further studies characterized by a better methodology, adequate sample size and longer follow-up are warranted in the next future., Registration: The protocol of this scoping review was registered in the International Prospective Register of Systematic Reviews (https://www.crd.york.ac.uk/PROSPERO) with ID: CRD42023391589., (© 2024 Wiley Periodicals LLC.)
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- 2024
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139. Whole-body Diffusion-weighted Magnetic Resonance Imaging for Assessment of the Bone Response Rate in Patients with Metastatic Hormone-sensitive Prostate Cancer Receiving Enzalutamide.
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Dalla Volta A, Valcamonico F, Zivi A, Procopio G, Sepe P, Del Conte G, Di Meo N, Foti S, Zamboni S, Messina C, Lucchini E, Rizzi A, Ravanelli M, Calza S, Zacchi F, Ciccone G, Suardi N, Maroldi R, Farina D, and Berruti A
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- Humans, Male, Whole Body Imaging, Antineoplastic Agents therapeutic use, Aged, Nitriles therapeutic use, Phenylthiohydantoin therapeutic use, Phenylthiohydantoin analogs & derivatives, Benzamides, Prostatic Neoplasms drug therapy, Prostatic Neoplasms pathology, Prostatic Neoplasms diagnostic imaging, Diffusion Magnetic Resonance Imaging, Bone Neoplasms secondary, Bone Neoplasms drug therapy, Bone Neoplasms diagnostic imaging
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- 2024
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140. Is precision medicine the solution to improve organ preservation in laryngeal/hypopharyngeal cancer? A position paper by the Preserve Research Group.
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Mattavelli D, Wichmann G, Smussi D, Paderno A, Plana MS, Mesia RN, Compagnoni M, Medda A, Chiocca S, Calza S, Zhan Y, Rognoni C, Tarricone R, Stucchi E, Lorini L, Gurizzan C, Khelik K, Hovig E, Dietz A, Piazza C, and Bossi P
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In locally advanced (LA) laryngeal/hypopharyngeal squamous cell carcinoma (LHSCC), larynx preservation (LP) strategies aim at the cure of the disease while preserving a functional larynx, thus avoiding total laryngectomy and the associated impact on the quality of life. In the last decades, apart from transoral and open-neck organ preservation approaches, several non-surgical regimens have been investigated: radiotherapy alone, alternate, concurrent or sequential chemoradiation, and bioradiotherapy. Despite major progress, the identification of reliable and effective predictors for treatment response remains a clinical challenge. This review examines the current state of LP in LA-LHSCC and the need for predictive factors, highlighting the importance of the PRESERVE trial in addressing this gap. The PRESERVE trial represents a pivotal initiative aimed at finding the optimal therapy for laryngeal preservation specific to each patient through a retrospective analysis of data from previous LP trials and prospectively validating findings. The goal of the PRESERVE trial is to develop a comprehensive predictive classifier that integrates clinical, molecular, and multi-omics data, thereby enhancing the precision and efficacy of patient selection for LP protocols., Competing Interests: PB: participation in an advisory board or conference honoraria for: Merck, Sanofi-Regeneron, Merck Sharp & Dohme, Glaxo Smith Kline, Merus, Pfizer, Sun Pharma, Angelini, Nestlè. MP: Eisai, Invited Speaker, MSD, Invited Speaker, Travel grants. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Mattavelli, Wichmann, Smussi, Paderno, Plana, Mesia, Compagnoni, Medda, Chiocca, Calza, Zhan, Rognoni, Tarricone, Stucchi, Lorini, Gurizzan, Khelik, Hovig, Dietz, Piazza and Bossi.)
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- 2024
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141. A qualitative study of pediatric nurses' perception of factors affecting negotiation of care in a Pediatric Stem Cell Transplant Unit.
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Calza S, Da Rin Della Mora R, Todeschini A, Petralia P, and Scelsi S
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- Humans, Female, Male, Child, Adult, Pediatric Nursing, Communication, Parents psychology, Interviews as Topic, Middle Aged, Negotiating, Qualitative Research, Stem Cell Transplantation, Attitude of Health Personnel, Nurses, Pediatric psychology
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Background: The adoption of a "family centered care" (FCC) philosophy is essential for the care process and its negotiation. A better understanding of nurses' perception of factors that affect the process of negotiation could allow us to better address future interventions and to improve FCC. The purpose of our study was to investigate pediatric nurses' perception of factors that affect the process of negotiation of care with stem cell transplantation pediatric patients and their parents., Methods: A qualitative research design with in-depth interviews was chosen. Sixteen interviews (16 nurses) were audio recorded and transcribed verbatim. Two researchers conducted independently a thematic analysis of the verbatim transcripts of the interviews., Results: Four themes emerged from the data as factors that affect the process of negotiation: 1) communication; 2) personal factors; 3) specificity; and 4) organization., Conclusions: These themes represent interesting points for future improvement interventions. Negotiation in the stem cell transplant setting would deserve further research, with special focus on children' and parents' perception of factors affecting this important aspect. Furthermore, in the future, negotiation guidelines could be validated and implemented effectively and an already validated tool could be used to document the negotiation process in the stem cell transplant setting.
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- 2024
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142. The effect of weight gain and metabolic dysfunction-associated steatotic liver disease on liver fibrosis progression and regression in people with HIV.
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Guaraldi G, Milic J, Renzetti S, Motta F, Cinque F, Bischoff J, Desilani A, Conti J, Medioli F, Del Monte M, Kablawi D, Elgretli W, Calza S, Mussini C, Rockstroh JK, and Sebastiani G
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- Humans, Male, Female, Middle Aged, Adult, Cohort Studies, HIV Infections complications, Liver Cirrhosis, Weight Gain, Disease Progression, Fatty Liver pathology
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Objective: People with HIV (PWH) have high risk of liver fibrosis. We investigated the effect of weight gain and metabolic dysfunction-associated steatotic liver disease (MASLD) on liver fibrosis dynamics., Design: Multicenter cohort study., Methods: Fibrosis progression was defined as development of significant fibrosis [liver stiffness measurement (LSM) ≥8 kPa], or transition to cirrhosis (LSM ≥13 kPa), for those with significant fibrosis at baseline. Fibrosis regression was defined as transition to LSM less than 8 kPa, or to LSM less than 13 kPa for those with cirrhosis at baseline. MASLD was defined as hepatic steatosis (controlled attenuation parameter >248 dB/m) with at least one metabolic abnormality. A continuous-time multistate Markov model was used to describe transitions across fibrosis states., Results: Among 1183 PWH included from three centers (25.2% with viral hepatitis coinfection), baseline prevalence of significant fibrosis and MASLD was 14.4 and 46.8%, respectively. During a median follow-up of 2.5 years (interquartile range 1.9-3.5), the incidence rate of fibrosis progression and regression was 2.8 [95% confidence interval (CI) 2.3-3.4] and 2.2 (95% CI 1.9-2.6) per 100 person-years, respectively. In Markov model, weight gain increased the odds of fibrosis progression [odds ratio (OR) 3.11, 95% CI 1.59-6.08], whereas weight gain (OR 0.30, 95% CI 0.10-0.84) and male sex (OR 0.32, 95% CI 0.14-0.75) decreased the odds of fibrosis regression. On multivariable Cox regression analysis, predictors of fibrosis progression were weight gain [adjusted hazard ratio (aHR) 3.12, 95% CI 1.41-6.90] and MASLD (aHR 2.72, 95% CI 1.05-7.02)., Conclusion: Fibrosis transitions are driven by metabolic health variables in PWH, independently of viral hepatitis coinfection and antiretroviral class therapy., (Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc.)
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- 2024
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143. A multi-environmental source approach to explore associations between metals exposure and olfactory identification among school-age children residing in northern Italy.
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Renzetti S, van Thriel C, Lucchini RG, Smith DR, Peli M, Borgese L, Cirelli P, Bilo F, Patrono A, Cagna G, Rechtman E, Idili S, Ongaro E, Calza S, Rota M, Wright RO, Claus Henn B, Horton MK, and Placidi D
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- Humans, Female, Child, Male, Italy, Adolescent, Smell, Olfaction Disorders chemically induced, Dust analysis, Environmental Monitoring methods, Metals, Heavy analysis, Air Pollutants analysis, Air Pollutants adverse effects, Environmental Exposure analysis, Environmental Exposure adverse effects, Metals analysis
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Background: Metal exposures can adversely impact olfactory function. Few studies have examined this association in children. Further, metal exposure occurs as a mixture, yet previous studies of metal-associated olfactory dysfunction only examined individual metals. Preventing olfactory dysfunctions can improve quality of life and prevent neurodegenerative diseases with long-term health implications., Objective: We aimed to test the association between exposure to a mixture of 12 metals measured in environmental sources and olfactory function among children and adolescents residing in the industrialized province of Brescia, Italy., Methods: We enrolled 130 children between 6 and 13 years old (51.5% females) and used the "Sniffin' Sticks" test to measure olfactory performance in identifying smells. We used a portable X-ray fluorescence instrument to determine concentrations of metals (arsenic (As), calcium, cadmium (Cd), chromium, copper, iron, manganese, lead (Pb), antimony, titanium, vanadium and zinc) in outdoor and indoor deposited dust and soil samples collected from participants' households. We used an extension of weighted quantile sum (WQS) regression to test the association between exposure to metal mixtures in multiple environmental media and olfactory function adjusting for age, sex, socio-economic status, intelligence quotient and parents' smoking status., Results: A higher multi-source mixture was significantly associated with a reduced Sniffin' Sticks identification score (β = -0.228; 95% CI -0.433, -0.020). Indoor dust concentrations of Pb, Cd and As provided the strongest contributions to this association (13.8%, 13.3% and 10.1%, respectively). The metal mixture in indoor dust contributed more (for 8 metals out of 12) to the association between metals and olfactory function compared to soil or outdoor dust., Impact Statement: Among a mixture of 12 metals measured in three different environmental sources (soil, outdoor and indoor dust), we identified Pb, Cd and As measured in indoor dust as the main contributors to reduced olfactory function in children and adolescents residing in an industrialized area. Exposure to indoor pollution can be effectively reduced through individual and public health interventions allowing to prevent the deterioration of olfactory functions. Moreover, the identification of the factors that can deteriorate olfactory functions can be a helpful instrument to improve quality of life and prevent neurodegenerative diseases as long-term health implications., (© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.)
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- 2024
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144. Effects of Limbs' Spasticity on Spinopelvic Alignment in Post-Stroke Patients: A Cross-Sectional Study.
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Bissolotti L, Brojka A, Vezzoli M, Calza S, Nicoli F, Romero-Morales C, and Villafañe JH
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Objectives : This study aimed to determine the impacts of upper and lower limb (UL and LL) spasticity and impairment on spinal alignment in chronic post-stroke patients. Methods : A total of 45 consecutive chronic post-stroke patients, 18 women and 27 men, from 18 to 70 years old who presented post-stroke hemiparesis were recruited in this cross-sectional study. The clinical assessment included the Modified Ashworth Scale (UL-MAS and LL-MAS spasticity), Upper Limb Motricity Index (UL-MI), FAST-UL, and Five Times Sit-to-Stand Test (5T-STS); the Associated Reaction Rating Scale was used to measure associated reactions in the hemiparetic UL, the plumb line distance from the spinous process of C7 on the sagittal (PL-C7s) and frontal plane (Pl-C7f), the kyphosis apex (PL-AK), and the spinous process of L3 (PL-L3). Angular measures of spinal alignment were measured by a Bunnell scoliometer™ (angle of trunk rotation-ATR) and a gravity-dependent inclinometer (inclination at C7-T1 and T12-L1). Results : In chronic post-stroke patients, there was found to be an association between the 5T-STS and PL-C7f (β = 0.41, p = 0.05) and the angle of inclination at T12-L1 (β = 0.44, p = 0.01). The FAST-UL correlated with PL-C7f (β = -0.41, p = 0.05), while the UL-MI correlated with this last parameter (β = -0.36, p = 0.04) and the ATR (β = -0.31, p = 0.05). The UL-MAS showed correlation with the ATR (β = 0.38, p = 0.01). Conclusions : The results lead to the possibility that, in chronic post-stroke patients, spinal misalignment on the frontal and sagittal plane is associated both with strength impairment and UL spasticity. The improvement or restoration of spinopelvic parameters can take advantage of therapeutic interventions targeted at motor improvement and spasticity reduction of the hemiparetic side.
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- 2024
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145. Academic skills in children with cerebral palsy and specific learning disorders.
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Micheletti S, Galli J, Vezzoli M, Scaglioni V, Agostini S, Calza S, Merabet LB, and Fazzi E
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- Humans, Child, Male, Female, Cross-Sectional Studies, Prospective Studies, Neuropsychological Tests, Adolescent, Academic Performance, Learning Disabilities etiology, Cerebral Palsy complications, Specific Learning Disorder
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Aim: To investigate the prevalence and clinical manifestations of reading, writing, and mathematics disorders in children with cerebral palsy (CP). We explored how the clinical profile of these children differed from those with specific learning disorders (SLDs), taking into account several factors, particularly IQ scores, neuropsychological aspects, and the presence of a visual impairment., Method: A prospective cross-sectional study was conducted in 42 children with CP (mean age 9 years 8 months; SD = 2 years 2 months) and 60 children with SLDs (mean age 10 years; SD = 1 year 7 months). Clinical characteristics, neuromotor and cognitive profiles, neuropsychological aspects (speech performance, academic skills, visual attention, phonological awareness, working memory), and signs of visual impairment (visual acuity, contrast sensitivity, visual field, oculomotor functions) were assessed. A machine learning approach consisting of a random forest algorithm, where the outcome was the diagnosis and the covariates were the clinical variables collected in the sample, was used for the analyses., Results: About 59% of the children with CP had reading, writing, or mathematics disorders. Children with CP with learning disorders had a low performance IQ, normal phonological awareness, and working memory difficulties, whereas children with SLDs had normal performance IQ, impaired phonological awareness, and mild working memory difficulties. There were no differences in verbal IQ between the two groups., Interpretation: Learning disorders are frequently associated with CP, with different clinical characteristics, compared with SLDs. Assessment of academic skills is mandatory in these children, even if the IQ is normal. At school age, specific interventions to promote academic skills in children with CP could be a major rehabilitative goal., What This Paper Adds: Reading, writing, and mathematics disorders in cerebral palsy have specific clinical characteristics. Their underlying mechanisms differ from those described in specific learning disorders. Working memory impairment can be considered a hallmark of learning disorders in children with cerebral palsy., (© 2023 The Authors. Developmental Medicine & Child Neurology published by John Wiley & Sons Ltd on behalf of Mac Keith Press.)
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- 2024
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146. Gender-Specific Differences in Spinal Alignment and Muscle Power in Patients with Parkinson's Disease.
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Bissolotti L, Rota M, Calza S, Romero-Morales C, Alonso-Pérez JL, López-Bueno R, and Villafañe JH
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Background: Parkinson's disease (PD) is an advancing neurodegenerative disorder characterized by spinal anomalies and muscular weakness, which may restrict daily functional capacities. A gender-focused examination of these effects could provide valuable insights into customized rehabilitation strategies for both sexes., Purpose: This study investigates the influence of spinal alignment on lower-limb function during the sit-to-stand (STS) movement in patients with Parkinson's disease compared to healthy individuals., Methods: A cross-sectional study was conducted with 43 consecutive patients with PD (25 males and 18 females; average age 73.7 ± 7.1 years) and 42 healthy controls (22 males and 20 females; average age 69.8 ± 6.0 years). Assessments included the International Physical Activity Questionnaire (IPAQ), Hoehn and Yahr staging, and measurements of vertical deviations from several spinal landmarks. Lower-limb muscle power during the STS task was evaluated using the Muscle Quality Index (MQI)., Results: Both absolute (Watts) and relative (Watts/Kg) muscle power in the lower limbs were notably decreased in the PD group compared to the control group. Within the PD cohort, muscle power showed a negative relationship with age and a positive association with the degree of lumbar lordosis (PL-L3). Importantly, gender-specific analysis revealed that male patients with PD had significantly higher lower-limb muscle power compared to female patients with PD, highlighting the need for gender-tailored therapeutic approaches., Conclusions: The findings suggest that preserving lumbar lordosis is crucial for maintaining effective lower-limb muscle biomechanics in individuals with Parkinson's disease.
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- 2024
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147. Medical comorbidities in bipolar disorder (BIPCOM): clinical validation of risk factors and biomarkers to improve prevention and treatment. Study protocol.
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de Girolamo G, Andreassen OA, Bauer M, Brambilla P, Calza S, Citerà N, Corcoy R, Fagiolini A, Garcia-Argibay M, Godin O, Klingler F, Kobayashi NF, Larsson H, Leboyer M, Matura S, Martinelli A, De la Peña-Arteaga V, Poli R, Reif A, Ritter P, Rødevand LN, Magno M, and Caselani E
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Background: BIPCOM aims to (1) identify medical comorbidities in people with bipolar disorder (BD); (2) examine risk factors and clinical profiles of Medical Comorbidities (MC) in this clinical group, with a special focus on Metabolic Syndrome (MetS); (3) develop a Clinical Support Tool (CST) for the personalized management of BD and medical comorbidities., Methods: The BIPCOM project aims to investigate MC, specifically MetS, in individuals with BD using various approaches. Initially, prevalence rates, characteristics, genetic and non-genetic risk factors, and the natural progression of MetS among individuals with BD will be assessed by analysing Nordic registers, biobanks, and existing patient datasets from 11 European recruiting centres across 5 countries. Subsequently, a clinical study involving 400 participants from these sites will be conducted to examine the clinical profiles and incidence of specific MetS risk factors over 1 year. Baseline assessments, 1-year follow-ups, biomarker analyses, and physical activity measurements with wearable biosensors, and focus groups will be performed. Using this comprehensive data, a CST will be developed to enhance the prevention, early detection, and personalized treatment of MC in BD, by incorporating clinical, biological, sex and genetic information. This protocol will highlight the study's methodology., Discussion: BIPCOM's data collection will pave the way for tailored treatment and prevention approaches for individuals with BD. This approach has the potential to generate significant healthcare savings by preventing complications, hospitalizations, and emergency visits related to comorbidities and cardiovascular risks in BD. BIPCOM's data collection will enhance BD patient care through personalized strategies, resulting in improved quality of life and reduced costly interventions. The findings of the study will contribute to a better understanding of the relationship between medical comorbidities and BD, enabling accurate prediction and effective management of MetS and cardiovascular diseases., Trial Registration: ISRCTN68010602 at https://www.isrctn.com/ISRCTN68010602 . Registration date: 18/04/2023., (© 2024. The Author(s).)
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- 2024
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148. Metabolic Syndrome in people treated with Antipsychotics (RISKMet): A multimethod study protocol investigating genetic, behavioural, and environmental risk factors.
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de Girolamo G, La Cascia C, Macchia PE, Nobile M, Calza S, Camillo L, Mauri M, Pozzi M, Tripoli G, Vetrani C, Caselani E, and Magno M
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- Humans, Risk Factors, Male, Female, Adult, Case-Control Studies, Prospective Studies, Life Style, Exercise, Middle Aged, Child, Metabolic Syndrome chemically induced, Metabolic Syndrome genetics, Antipsychotic Agents adverse effects, Antipsychotic Agents therapeutic use
- Abstract
Introduction: The RISKMet project aims to: (1) identify risk factors for metabolic syndrome (MetS) by comparing patients with and without MetS; (2) characterise patients treated with second-generation antipsychotics (SGAs) about MetS diagnosis; (3) study behavioural patterns, including physical activity (PA) and dietary habits, in patients and healthy individuals using a prospective cohort design., Method: The RISKMet project investigates MetS in individuals treated with SGAs, focusing on both adult and paediatric populations. The study utilizes a case-control design to examine potential risk factors for MetS, categorizing participants as MetS+ considered as "Cases" and MetS- considered as "Controls" matched by sex and age. The evaluation of factors such as MetS, lifestyle habits, and environmental influences is conducted at two time points, T0 and T3, after 3 months. Subsequently, the project aims to assess body parameters, including physical examinations, and blood, and stool sample collection, to evaluate metabolic markers and the impact of SGAs. The analysis includes pharmacological treatment data and genetic variability. Behavioural markers related to lifestyle, eating behaviour, PA, and mood are assessed at both T0 and T3 using interviews, accelerometers, and a mobile app. The study aims to improve mental and physical well-being in SGA-treated individuals, establish a biobank for MetS research, build an evidence base for physical health programs, and develop preventive strategies for SGA-related comorbidities., Conclusions: This project innovates MetS monitoring in psychiatry by using intensive digital phenotyping, identifying biochemical markers, assessing familial risks, and including genetically similar healthy controls., Study Registration Number: ISRCTN18419418 at www.isrctn.com., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 de Girolamo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2024
- Full Text
- View/download PDF
149. Topical Antiseptics in Minimizing Ocular Surface Bacterial Load Before Ophthalmic Surgery: A Randomized Controlled Trial.
- Author
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Romano V, Ferrara M, Gatti F, Airaldi M, Borroni D, Aragona E, Rocha-de-Lossada C, Gabrielli F, Papa FT, Romano MR, Calza S, and Semeraro F
- Subjects
- Adult, Humans, Bacterial Load, Povidone-Iodine, Chlorhexidine therapeutic use, Conjunctiva microbiology, Ophthalmic Solutions, Anti-Infective Agents, Local, Ophthalmology
- Abstract
Purpose: To investigate the reduction of the ocular surface bacterial load induced by 2 commercially available ophthalmic antiseptic formulations, povidone-iodine (PVI) 0.6% and chlorhexidine (CLX) 0.02%, before ocular surgery., Design: Randomized controlled trial., Methods: Seventy adult patients undergoing intraocular surgery (phacoemulsification) were randomized to receive in the index eye PVI (group A) 4 times a day for 3 days or CLX (group B) 4 times a day for 3 days before surgery. The untreated eye was used as control. A conjunctival swab was taken in both eyes before (T0) and after (T1) therapy. Microbial DNA was quantified with real-time polymerase chain reaction (PCR) analysis. The Mick algorithm was used to compare the abundance of each genus/genera against the distribution of abundances from the reference. At T1, patients filled a questionnaire to evaluate therapy-induced symptoms. Primary outcome was the reduction of bacterial DNA at T1 (microbial load), vs control arm, expressed as mean number of real-time PCR cycle times (CTs). Secondary outcomes were taxonomic composition, differential abundance, and therapy-induced ocular symptoms., Results: The T0-T1 difference in CT was significant in group B, but not in group A (mean [95% CI], 0.99 [0.33] vs 0.26 [0.15], P < .001, and 0.65 [0.3] vs 0.45 [0.41], P = .09, respectively). The taxonomic composition, alpha, and beta diversity remained consistent at all time points in both groups. The rate of patients reporting therapy-induced ocular symptoms and the mean discomfort grade were greater in group A than in group B (97% vs 26% and 4.97±2.48 vs 0.66±1.53, respectively)., Conclusions: Compared with PVI 0.6%, CLX 0.02% induced a greater reduction of ocular surface bacterial load, with no significant alterations of the taxonomic composition. Moreover, CLX was better tolerated than PVI., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
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- 2024
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150. Space For Children: improving mastery and reducing negative emotions about COVID-19 in youth via mobile interactive storytelling.
- Author
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Scelsi S, Capuozzo P, Primavera L, Calza S, DA Rin Della Mora R, Bandelloni L, Biolcati Rinaldi A, Cimellaro P, D'Apruzzo M, DI Profio S, Parodi A, Rebora S, Serveli S, Tani D, Viacava R, and Venturino C
- Abstract
Background: The COVID-19 pandemic has been a complex event for children and adolescents, significantly impacting their daily activities. In this scenario, our study aims at verifying if "Space for Children," an ad-hoc developed game based on interactive storytelling about the pandemic, can increase mastery and reduces negative emotions about COVID-19., Methods: The app Space for Children has been sponsored and spread online by email and social media platforms for a limited time period (from May 2021 to January 2022). Before and after the interactive storytelling, participants answered two questionnaires containing a couple of critical questions regarding their emotional state and their perceived mastery about COVID-19., Results: Two hundred thirty-six participants (M=116; F=120) between 7 and 12 years old completed the Space for Children interactive experience. Our results show a significant effect of the game experience on emotional state and perceived mastery regarding COVID-19. Indeed, before the interactive storytelling unpleasant emotional states prevail (reported by 77% of participants) while after the game a clear prevalence of pleasant emotions emerges (reported by 68% of participants). Regarding perceived mastery, results show that self-reported very expert participants passed from 49% to 88%. All the results hold irrespective of age group and sex., Conclusions: This study presents preliminary findings that highlight the potential benefits of utilizing new technologies based on Interactive Storytelling for Children to effectively convey age-appropriate information about complex real-life events, such as the COVID-19 pandemic, while also mitigating associated negative emotional responses.
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- 2024
- Full Text
- View/download PDF
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