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586 results on '"Bougnoux, Marie‐Elisabeth"'

101. Antimicrobial resistance in commensal flora of pig farmers

Author

Audry-Damon, Helene, Grenet, Karine, Sall-Ndiaye, Penda, Che, Didier, Cordeiro, Eugenio, Bougnoux, Marie-Elisabeth, Rigaud, Emma, Le Strat, Yann, Lemanissier, Veronique, Armand-Lefevre, Laurence, Delzescaux, Didier, Desenclos, Jean-Claude, Lienard, Michel, and Andremont, Antoine

Subjects
Farmers -- Health aspects, Farmers -- Research, Anti-infective agents -- Research
Abstract

We assessed the quantitative contribution of pig farming to antimicrobial resistance in the commensal flora of pig farmers by comparing 113 healthy pig farmers from the major French porcine production [...]

Published
2004

102. Deep Dermatophytosis and Inherited CARD9 Deficiency

Author

Lanternier, Fanny, Pathan, Saad, Vincent, Quentin B., Liu, Luyan, Cypowyj, Sophie, Prando, Carolina, Migaud, Mélanie, Taibi, Lynda, Ammar-Khodja, Aomar, Stambouli, Omar Boudghene, Guellil, Boumediene, Jacobs, Frederique, Goffard, Jean-Christophe, Schepers, Kinda, del Marmol, Véronique, Boussofara, Lobna, Denguezli, Mohamed, Larif, Molka, Bachelez, Hervé, Michel, Laurence, Lefranc, Gérard, Hay, Rod, Jouvion, Gregory, Chretien, Fabrice, Fraitag, Sylvie, Bougnoux, Marie-Elisabeth, Boudia, Merad, Abel, Laurent, Lortholary, Olivier, Casanova, Jean-Laurent, Picard, Capucine, Grimbacher, Bodo, and Puel, Anne

Published
2013
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110. Invasive Aspergillosis Due to Aspergillus Section Usti: A Multicenter Retrospective Study

Author

Glampedakis, Emmanouil, primary, Cassaing, Sophie, additional, Fekkar, Arnaud, additional, Dannaoui, Eric, additional, Bougnoux, Marie-Elisabeth, additional, Bretagne, Stéphane, additional, Neofytos, Dionysios, additional, Schreiber, Peter W, additional, Hennequin, Christophe, additional, Morio, Florent, additional, Shadrivova, Olga, additional, Bongomin, Felix, additional, Fernández-Ruiz, Mario, additional, Bellanger, Anne Pauline, additional, Arikan-Akdagli, Sevtap, additional, Erard, Veronique, additional, Aigner, Maria, additional, Paolucci, Michela, additional, Khanna, Nina, additional, Charpentier, Eléna, additional, Bonnal, Christine, additional, Brun, Sophie, additional, Gabriel, Frederic, additional, Riat, Arnaud, additional, Zbinden, Reinhard, additional, Le Pape, Patrice, additional, Klimko, Nikolai, additional, Lewis, Russel E, additional, Richardson, Malcolm, additional, İnkaya, Ahmet Cagkan, additional, Coste, Alix T, additional, Bochud, Pierre-Yves, additional, and Lamoth, Frederic, additional

Published
2020
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111. Aspergillus fumigatus Infection in Humans With STAT3-Deficiency Is Associated With Defective Interferon-Gamma and Th17 Responses

Author

Danion, François, primary, Aimanianda, Vishukumar, additional, Bayry, Jagadeesh, additional, Duréault, Amélie, additional, Wong, Sarah Sze Wah, additional, Bougnoux, Marie-Elisabeth, additional, Tcherakian, Colas, additional, Alyanakian, Marie-Alexandra, additional, Guegan, Hélène, additional, Puel, Anne, additional, Picard, Capucine, additional, Lortholary, Olivier, additional, Lanternier, Fanny, additional, and Latgé, Jean-Paul, additional

Published
2020
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112. Extracellular ATP released from Candida albicans activates non-peptidergic neurons to augment host defense

Author

Edwards, Tara N, primary, Zhang, Shiqun, additional, Liu, Andrew, additional, Cohen, Jonathan A., additional, Zhou, Paul Yifan, additional, Mogavero, Selene, additional, Hube, Bernhard, additional, Berman, Judith, additional, Bougnoux, Marie-Elisabeth, additional, Mathers, Alicia R., additional, Gaffen, Sarah L., additional, Albers, Kathryn M., additional, Koerber, H. Richard, additional, Davis, Brian M., additional, D’Enfert, Christophe, additional, and Kaplan, Daniel H, additional

Published
2020
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113. Large-scale genome mining allows identification of neutral polymorphisms and novel resistance mutations in genes involved in Candida albicans resistance to azoles and echinocandins

Author

Sitterlé, Emilie, primary, Coste, Alix T, primary, Obadia, Thomas, primary, Maufrais, Corinne, primary, Chauvel, Murielle, primary, Sertour, Natacha, primary, Sanglard, Dominique, primary, Puel, Anne, primary, D’Enfert, Christophe, primary, and Bougnoux, Marie-Elisabeth, primary

Published
2020
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115. Diagnostic accuracy of serum (1,3)-beta-d-glucan for neonatal invasive candidiasis: systematic review and meta-analysis

Author

Cohen, Jérémie, Ouziel, Antoine, Matczak, Soraya, Brice, Josephine, Spijker, Rene, Lortholary, Olivier, Bougnoux, Marie-Elisabeth, Toubiana, Julie, Equipe 1 : EPOPé - Épidémiologie Obstétricale, Périnatale et Pédiatrique (CRESS - U1153), Université Paris Descartes - Paris 5 (UPD5)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Pédiatrie et maladies infectieuses [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Julius Center for Health Sciences and Primary Care, University Medical Center [Utrecht], Medical Library [Amsterdam, The Netherlands] (Amsterdam Public Health), University of Amsterdam [Amsterdam] (UvA), Service des Maladies infectieuses et tropicales [CHU Necker], Université Sorbonne Paris Cité (USPC), Mycologie moléculaire - Molecular Mycology, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals (CNRMA), Institut Pasteur [Paris], Département de Mycologie [Hôpital Necker-Enfants malades - APHP], and Biodiversité et Epidémiologie des Bactéries pathogènes - Biodiversity and Epidemiology of Bacterial Pathogens

Subjects
Sensitivity, Diagnostic tests, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Systematic review, Specificity, Neonatal invasive candidiasis, (13)-Beta-d-glucan
Abstract

Jérémie F. Cohen and Antoine Ouziel are equal contribution.; International audience; OBJECTIVES: Neonatal invasive candidiasis (NIC) is a leading cause of infection-related morbidity and mortality in preterm neonates. Several studies have shown that (1,3)-Beta-d-glucan (BDG) was accurate in detecting invasive fungal infection in adults, but studies in neonates are scarce. The aim was to obtain summary estimates of the accuracy of BDG detection in serum for the diagnosis of NIC.METHODS: We searched Medline, Embase, Clinicaltrials.gov, and Google Scholar (inception to July 2019). We checked the reference lists of included studies, clinical guidelines, and review articles. We included studies that assessed the accuracy of BDG against a reference standard that defined groups of patients with ordinal levels of NIC probability (e.g. proven, probable, possible) and included fungal blood culture. Participants were neonates suspected of having NIC. The intervention was BDG measurement in serum (Fungitell® assay). We assessed risk of bias and applicability using QUADAS-2. We used bivariate meta-analysis to produce summary estimates of diagnostic accuracy at prespecified positivity thresholds of 80 and 120 pg/mL. This study was registered with PROSPERO (CRD42018089545).RESULTS: We included eight studies (465 participants). Of these, two were judged at low overall risk of bias. There was substantial variability across studies in the reference standards used. At a positivity threshold of 80 pg/mL, summary estimates of sensitivity and specificity of BDG were 89% (95% CI: 80-94%) and 60% (53-66%), respectively; summary sensitivity for detecting proven cases of NIC was 99% (93-100%). At a positivity threshold of 120 pg/mL, summary estimates of sensitivity and specificity were 81% (71-88%) and 80% (67-88%), respectively.CONCLUSIONS: Because of high sensitivity, BDG seems promising to rule-out NIC. It might be too early to recommend its use because of the scarcity of reliable clinical data, heterogeneity in case definitions, and unstable accuracy estimates.

Published
2019

117. Prosthetic Valve Candida spp. Endocarditis: New Insights Into Long-term Prognosis—The ESCAPE Study

Author

Rivoisy, Claire, Vena, Antonio, Schaeffer, Laura, Charlier, Caroline, Fontanet, Arnaud, Delahaye, François, Bouza, Emilio, Lortholary, Olivier, Lefort, Agnès, Yazdanpanah, Yazdan, Wolff, Michel, Houze, Sandrine, Ferry, Tristan, Wallon, Martine, Strady, Christophe, Toubas, Dominique, Bréchot, Nicolas, Henry, Benoit, Fekkar, Arnaud, Fourme, Thierry, Amouroux, Isabelle, Mainardi, Jean-Luc, Dannaoui, Eric, Rogeaux, Olivier, Levast, Marion, Chirouze, Catherine, Million, Laurence, Bastides, Frédéric, Chandenier, Jacques, Lançon, Jean-Pierre, Ovize, Anne, Abgueguen, Pierre, Bouchara, Jean-Philippe, Tric, Laurent, Habib, Gilbert, Gourriet, Frederic, Aaron, Laurent, Bougnoux, Marie Elisabeth, Camou, Fabrice, Accoceberry, Isabelle, Sánchez, Fernando Fernández, Noureddine, Mariam, Rosas, Gabriel, de la Torre Lima, Javier, Aramendi, José, Bereciartua, Elena, Victoria Boado, María, Campaña Lázaro, Marta, Goikoetxea, Josune, José Goiti, Juan, Hernández, José Luis, Iruretagoyena, José Ramón, Zuazabal, Josu Irurzun, López-Soria, Leire, Montejo, Miguel, Pérez, Pedro María, Rodríguez, Regino, Voces, Roberto, López, Mª Victoria García, Georgieva, Radka Ivanova, Solero, Manuel Márquez, Bailón, Isabel Rodríguez, Morales, Josefa Ruiz, Cuende, Ana María, Echeverría, Tomás, Fuerte, Ana, Gaminde, Eduardo, Goenaga, Miguel Ángel, Idígoras, Pedro, Iribarren, José Antonio, Yarza, Alberto Izaguirre, Urkola, Xabier Kortajarena, Reviejo, Carlos, Carrasco, Rafael, Climent, Vicente, Llamas, Patricio, Merino, Esperanza, Plazas, Joaquín, Reus, Sergio, Álvarez, Nemesio, Bravo-Ferrer, Jose María, Castelo, Laura, Cuenca, José, Llinares, Pedro, Rey, Enrique Miguez, Mayo, María Rodríguez, Sánchez, Efrén, Regueiro, Dolores Sousa, Martinez, Francisco Javier, del Mar Alonso, Mª, Castro, Beatriz, Marrero, Dácil García, Durán, Mª del Carmen, Gómez, Mª Antonia Miguel, Calzada, Juan La, Nassar, Ibrahim, Ciezar, Antonio Plata, Iglesias, José Mª Reguera, Asensio, Víctor, Costas, Carlos, de la Hera, Jesús, Suárez, Jonnathan Fernández, Fraile, Lisardo Iglesias, Arguero, Víctor León, Menéndez, José López, MenciaBajo, Pilar, Morales, Carlos, Torrico, Alfonso Moreno, Palomo, Carmen, Martínez, Begoña Paya, Esteban, Ángeles Rodríguez, García, Raquel Rodríguez, Asensio, Mauricio Telenti, Almela, Manuel, Armero, Yolanda, Azqueta, Manuel, Brunet, Merce, Cartañá, Ramón, Cervera, Carlos, Falces, Carlos, Fita, Guillermina, Fuster, David, de la Maria, Cristina García, Gatell, José, Pérez, Jaume Llopis, Marco, Francesc, Mestres, Carlos, Miró, José Mª, Moreno, Asunción, Ninot, Salvador, Quintana, Eduardo, Paré, Carlos, Pericás, Juan Manuel, Pomar, José, Ramírez, José, Rovira, Irene, Sitges, Marta, Soy, Dolors, Téllez, Adrián, Vila, Jordi, Bermejo, Javier, Cuerpo, Gregorio, de Egea, Viviana, Eworo, Alia, Cruz, Ana Fernández, Leoni, Mª Eugenia García, del Vecchio, Marcela González, Ramallo, Víctor González, Hernández, Martha Kestler, Marín, Mercedes, Martínez-Sellés, Manuel, Menárguez, Mª Cruz, Muñoz, Patricia, Rincón, Cristina, Rodríguez-Abella, Hugo, Rodríguez-Créixems, Marta, Pinilla, Blanca, Pinto, Ángel, Valerio, Maricela, Moreno, Eduardo Verde, Antorrena, Isabel, Loeches, Belén, Moreno, Mar, Ramírez, Ulises, Bastón, Verónica Rial, Romero, María, Saldaña, Araceli, Castillo, Carlos Armiñanzas, Arnaiz, Ana, Berrazueta, José, Bellisco, Sara, Belaustegui, Manuel Cobo, Durán, Raquel, Fariñas, MªCarmen, Fariñas-Álvarez, Concepción, Mazarrasa, Carlos Fernández, Izquierdo, Rubén Gómez, Rico, Claudia González, Díez, José Gutiérrez, Durán, Rafael Martín, Pajarón, Marcos, Parra, José Antonio, Teira, Ramón, Zarauza, Jesús, Pavía, Pablo García, González, Jesús, Orden, Beatriz, RAMOS, Antonio, González, Elena Rodríguez, Centella, Tomasa, Hermida, José Manuel, Luis Moya, José, Martín-Dávila, Pilar, Navas, Enrique, Oliva, Enrique, del Río, Alejandro, Ruiz, Soledad, Tenorio, Carmen Hidalgo, de Castro, Antonio, de Cueto, Marina, Gallego, Pastora, Acebal, Juan Gálvez, Baño, Jesús Rodríguez, de Alarcón, Arístides, García, Emilio, Haro, Juan Luis, Lepe, José Antonio, López, Francisco, Luque, Rafael, Alonso, Luis Javier, Gutiérrez, José Manuel Azcona, Blanco, José Ramón, García, Lara, Oteo, José Antonio, de Benito, Natividad, Gurguí, Mercé, Pacho, Cristina, Pericas, Roser, Pons, Guillem, Alvarez, M., Fernández, A, Prieto, A., Regueiro, Benito, Tijeira, E, Vega, Marino, Blasco, Andrés Canut, Mollar, José Cordo, Arana, Juan Carlos Gainzarain, Uriarte, Oscar García, López, Alejandro Martín, de Zárate, Zuriñe Ortiz, Matos, José Antonio Urturi, Nacle, Mª Belén, Sánchez-Porto, Antonio, Vallejo, Luis, ArribasLeal, José Mª, Vázquez, Elisa García, Torres, Alicia Hernández, Blázquez, Ana, de la Morena Valenzuela, Gonzalo, Alonso, Ángel, Aramburu, Javier, Calvo, Felicitas Elena, Rodríguez, Anai Moreno, Tarabini-Castellani, Paola, Gálvez, Eva Heredero, Bellido, Carolina Maicas, Pau, José Largo, Sepúlveda, Mª Antonia, Sierra, Pilar Toledano, Iqbal-Mirza, Sadaf Zafar, Alcolea, Eva Cascales, Serrano, Pilar Egea, Hernández Roca, José Joaquín, Service des Maladies infectieuses et tropicales [CHU Necker], Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP]-Institut des Maladies Génétiques Imagine [Paris], Hospital General Universitario 'Gregorio Marañón' [Madrid], Epidémiologie des Maladies Emergentes, Pasteur-Cnam risques infectieux et émergents (PACRI), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Imagine - Institut des maladies génétiques (IMAGINE - U1163), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Centre d'infectiologie Necker-Pasteur [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Hôpital Beaujon, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Diderot - Paris 7 (UPD7)-Hôpital Beaujon, Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC), Mère et enfant face aux infections tropicales (MERIT - UMR_D 216), Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5), Service de Parasitologie, Hospices Civils de Lyon (HCL)-Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL), Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [APHP], Institut Élie Cartan de Lorraine (IECL), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière [APHP], Hôpital Européen Georges Pompidou [APHP] (HEGP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Mycologie moléculaire, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Service des Maladies Infectieuses, CHR Chambéry, Laboratoire de Bactériologie, Centre Hospitalier Régional de Chambéry, Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Service de Médecine Interne et Maladies Infectieuses [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), CHU Tours, Service de Parasitologie - Mycologie - Médecine Tropicale, Eurofins Biomnis [Lyon, France], Service des maladies infectieuses et tropicales [CHU Angers], Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Groupe d’Etude des Interactions Hôte-Pathogène, Université d'Angers (UA), Service de cardiologie, Université de la Méditerranée - Aix-Marseille 2-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Biologie et Pathogénicité fongiques, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris], Hôpital St-André, Réanimation, CHU Bordeaux [Bordeaux], Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Universitad Politecnica de Alicante (UPA), Departamento de Lenguajes y Sistemas Informaticos, Instituto Tecnologico de Aragon (ITA), Instituto Tecnológico de Aragón, Department of Anatomy and Cell Biology [Montréal], McGill University, Universitat de Barcelona (UB), AIDS Research Group [Barcelona, Spain], August Pi i Sunyer Biomedical Research Institute - IDIBAPS [Barcelona, Spain]-Hospital Clinic [Barcelona, Spain], Instituto Tecnológico de Tláhuac, Cardiology Department, Thorax Clinic Institute, Hospital Cliınic, Institut d'Investigacions Biomèdiques [Barcelona], Clinical Microbiology and Infectious Diseases Department, Universidad Complutense de Madrid [Madrid] (UCM), Universidade de Aveiro, Departamento de Quimica Organica [Universidad de Cordoba], Universidad de Córdoba [Cordoba], Department of Microbiology, Hospital de la Santa Creu i Sant Pau, University Rovirai Virgili de Tarragona (URV), Chercheur indépendant, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Centro de Investigación Biomédica en Red Enfermedades Respiratorias (CIBERES), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (MERIT - UMR_D 216), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Eurofins Biomnis, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP), Microbiologie Fondamentale et Pathogénicité (MFP), Instituto Tecnológico de Aragón (ITAINNOVA), McGill University = Université McGill [Montréal, Canada], Universidad de Córdoba = University of Córdoba [Córdoba], This work was supported by the Programa Multidisciplinar para la Gestión de Antifúngicos y la Reducción de Candidiasis Invasora (PROMULGA) II Project, Instituto de Salud Carlos III, partially financed by the European Regional Development Fund (grant PI13/01148). A. V. is supported by a Rio Hortega contract from the Instituto de Salud Carlos III, also partially financed by the European Regional Development Fund (grant CM15/00181)., Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Beaujon-Université Paris Diderot - Paris 7 (UPD7)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Hospital Clinic [Barcelona, Spain]-August Pi i Sunyer Biomedical Research Institute - IDIBAPS [Barcelona, Spain], and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Antifungal Agents, Prosthesis-Related Infections, Echinocandin, 030106 microbiology, 03 medical and health sciences, Candida, endocarditis, prosthetic valve, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Amphotericin B, Internal medicine, Humans, Medicine, Endocarditis, Prospective Studies, Registries, Adverse effect, Prospective cohort study, Fluconazole, Survival rate, ComputingMilieux_MISCELLANEOUS, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Aged, Retrospective Studies, business.industry, Candidiasis, Disease Management, Retrospective cohort study, Odds ratio, Middle Aged, Prognosis, medicine.disease, 3. Good health, Infectious Diseases, Spain, Heart Valve Prosthesis, Female, France, business, medicine.drug
Abstract

International audience; Background: Prosthetic valve endocarditis caused by Candida spp. (PVE-C) is rare and devastating, with international guidelines based on expert recommendations supporting the combination of surgery and subsequent azole treatment.Methods: We retrospectively analyzed PVE-C cases collected in Spain and France between 2001 and 2015, with a focus on management and outcome.Results: Forty-six cases were followed up for a median of 9 months. Twenty-two patients (48%) had a history of endocarditis, 30 cases (65%) were nosocomial or healthcare related, and 9 (20%) patients were intravenous drug users. "Induction" therapy consisted mainly of liposomal amphotericin B (L-amB)-based (n = 21) or echinocandin-based therapy (n = 13). Overall, 19 patients (41%) were operated on. Patients

Published
2018

118. Spectrum of Pulmonary Aspergillosis in Hyper-IgE Syndrome with Autosomal-Dominant STAT3 Deficiency

Author

Duréault, Amélie, primary, Tcherakian, Colas, additional, Poiree, Sylvain, additional, Catherinot, Emilie, additional, Danion, François, additional, Jouvion, Grégory, additional, Bougnoux, Marie Elisabeth, additional, Mahlaoui, Nizar, additional, Givel, Claire, additional, Castelle, Martin, additional, Picard, Capucine, additional, Chansdesris, Marie Olivia, additional, Lortholary, Olivier, additional, Lanternier, Fanny, additional, Coignard, H., additional, Amazzough, K., additional, Suarez, F., additional, Blanche, S., additional, Sendid, B., additional, Cornu, M., additional, Bervar, J.F., additional, Deschildre, A., additional, Wemeau, L., additional, Fieschi, C., additional, Alanio, A., additional, Menetrey, C., additional, Senechal, A., additional, Ader, F., additional, Tattevin, P., additional, Pison, C., additional, Grandiere-Perez, L., additional, Garcia-Hermoso, D., additional, and Botterel-Chartier, F., additional

Published
2019
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119. Why are so many cases of invasive aspergillosis missed?

Author

Danion, François, primary, Rouzaud, Claire, additional, Duréault, Amélie, additional, Poirée, Sylvain, additional, Bougnoux, Marie-Elisabeth, additional, Alanio, Alexandre, additional, Lanternier, Fanny, additional, and Lortholary, Olivier, additional

Published
2019
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120. Clinical Impact of Antifungal Susceptibility, Biofilm Formation and Mannoside Expression of Candida Yeasts on the Outcome of Invasive Candidiasis in ICU: An Ancillary Study on the Prospective AmarCAND2 Cohort

Author

Gangneux, Jean-Pierre, primary, Cornet, Muriel, additional, Bailly, Sébastien, additional, Fradin, Chantal, additional, Féger, Céline, additional, Timsit, Jean-François, additional, Leroy, Olivier, additional, Sendid, Boualem, additional, and Bougnoux, Marie-Elisabeth, additional

Published
2018
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121. Invasive Aspergillosis Due to Aspergillus Section Usti: A Multicenter Retrospective Study.

Author

Glampedakis, Emmanouil, Cassaing, Sophie, Fekkar, Arnaud, Dannaoui, Eric, Bougnoux, Marie-Elisabeth, Bretagne, Stéphane, Neofytos, Dionysios, Schreiber, Peter W, Hennequin, Christophe, Morio, Florent, Shadrivova, Olga, Bongomin, Felix, Fernández-Ruiz, Mario, Bellanger, Anne Pauline, Arikan-Akdagli, Sevtap, Erard, Veronique, Aigner, Maria, Paolucci, Michela, Khanna, Nina, and Charpentier, Eléna

Subjects
RESEARCH, MEDICAL cooperation, RETROSPECTIVE studies, ASPERGILLOSIS
Abstract

Background Aspergillus spp. of section Usti (A. ustus) represent a rare cause of invasive aspergillosis (IA). This multicenter study describes the epidemiology and outcome of A. ustus infections. Methods Patients with A. ustus isolated from any clinical specimen were retrospectively identified in 22 hospitals from 8 countries. When available, isolates were sent for species identification (BenA / CaM sequencing) and antifungal susceptibility testing. Additional cases were identified by review of the literature. Cases were classified as proven/probable IA or no infection, according to standard international criteria. Results Clinical report forms were obtained for 90 patients, of whom 27 had proven/probable IA. An additional 45 cases were identified from literature review for a total of 72 cases of proven/probable IA. Hematopoietic cell and solid-organ transplant recipients accounted for 47% and 33% cases, respectively. Only 8% patients were neutropenic at time of diagnosis. Ongoing antimold prophylaxis was present in 47% of cases. Pulmonary IA represented 67% of cases. Primary or secondary extrapulmonary sites of infection were observed in 46% of cases, with skin being affected in 28% of cases. Multiple antifungal drugs were used (consecutively or in combination) in 67% of cases. The 24-week mortality rate was 58%. A. calidoustus was the most frequent causal agent. Minimal inhibitory concentrations encompassing 90% isolates (MIC90) were 1, 8, >16, and 4 µg/mL for amphotericin B, voriconazole, posaconazole, and isavuconazole, respectively. Conclusions Aspergillus ustus IA mainly occurred in nonneutropenic transplant patients and was frequently associated with extrapulmonary sites of infection. Mortality rate was high and optimal antifungal therapy remains to be defined. [ABSTRACT FROM AUTHOR]

Published
2021
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122. Central nervous system candidiasis beyond neonates: Lessons from a nationwide study.

Author

Chaussade, Hélène, Cazals, Xavier, Desoubeaux, Guillaume, Jouvion, Gregory, Bougnoux, Marie-Elisabeth, Lefort, Agnes, Rivoisy, Claire, Desnos-Ollivier, Marie, Chretien, Fabrice, Chouaki, Taieb, Gruson, Bérengère, Bernard, Louis, Lortholary, Olivier, Lanternier, Fanny, and group, the French Mycosis study

Abstract

Though candidiasis is the most frequent invasive fungal infection, Candida spp. central nervous system (CNS) infections are rare but severe. To further describe clinico-patho-radiological presentations of this entity, we report a retrospective study from January 2005 to December 2018 including patients aged ≥ 28 days with proven or probable CNS candidiasis in France. Twenty-four patients were included. Seventeen patients (70%) had CNS localization secondary to disseminated candidiasis (10 with hematologic malignancies [HM]; the seven other patients had infective endocarditis [IE]). Among patients with HM, seven previously had lumbar puncture for intrathecal chemotherapy, the three others had IE. Among patients with disseminated infection, magnetic resonance imaging (MRI) evidenced meningitis (17%), micro-abscesses (58%), or vascular complications (67%). Seven patients (30%) had isolated CNS involvement related to neurosurgery (n  = 2), CARD9 deficiency (n  = 2), intravenous drug use, diabetes mellitus, or no identified predisposing condition (n  = 1 each). All evaluated patients with isolated CNS involvement had meningitis on cerebrospinal fluid (CSF) and intracranial hypertension. For the latter patients, MRI evidenced meningitis (71%) or abscesses (57%). Among all patients, cerebrospinal fluid (CSF) culture grew Candida spp. in 31% of cases. CSF βDGlucan or mannan Ag were positive in respectively 86% and 80% of cases. Mortality attributed to CNS candidiasis was 42%: 53% in case of disseminated infection (70% for HM) and 14% in case of localized infection. CNS candidiasis are isolated or occur during disseminated infection in patients with HM and lumbar puncture for intrathecal chemotherapy or during IE. Clinical, radiological finding and outcome highly vary according to CNS localized versus disseminated candidiasis. Lay Summary Candida is a yeast and is the most common cause of fungal infections worldwide. Candida central nervous system (CNS) infections are rare, severe, and poorly described. We report a retrospective study from January 2005 to December 2018 including patients aged ≥ 28 days with proven or probable CNS candidiasis in France. Twenty-four patients were included (14 men, median age 51 years). Seventeen patients had CNS localization secondary to disseminated candidiasis from blood to CNS (10 with hematologic malignancies [HM], the seven other patients had infective endocarditis [IE]). Seven patients had isolated CNS involvement related to neurosurgery (n  = 2), CARD9 deficiency (n  = 2), intravenous drug use (n  = 1), diabetes mellitus (n  = 1), or no identified risk factor (n  = 1). During Candida CNS infections, brain lesions were meningitis abscesses or vascular complications. Cerebrospinal fluid (CSF) culture grew Candida spp. in 31% of cases. Forty-two percent of patients died from infection: 53% in case of disseminated infection (70% for HM) and 14% in case of localized infection. [ABSTRACT FROM AUTHOR]

Published
2021
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123. Cryptosporidium spp. Infection in Solid Organ Transplantation

Author

Lanternier, Fanny, Amazzough, Karima, Favennec, Loïc, Mamzer-Bruneel, Marie-France, Abdoul, Hendy, Tourret, Jérôme, Decramer, Stéphane, Zuber, Julien, Scemla, Anne, Legendre, Christophe, Lortholary, Olivier, Bougnoux, Marie-Elisabeth, Rodier, Marie-Hélène, Centre d'infectiologie Necker-Pasteur [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Imagine - Institut des maladies génétiques (IMAGINE - U1163), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Appareil Digestif Environnement Nutrition (ADEN ), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Service de néphrologie adultes [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], Epidémiologie Clinique et Evaluation Economique Appliquées aux Populations Vulnérables (ECEVE (U1123 / UMR_S_1123)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AP-HP Hôpital universitaire Robert-Debré [Paris], Ecologie et Evolution des Microorganismes (EEM), Université Paris Diderot - Paris 7 (UPD7)-Université Paris 13 (UP13)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), Réseau CENTAURE, Service de Microbiologie, Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP], Ecologie et biologie des interactions (EBI), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Microbiologie de l'Eau (MDE), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Abstract

International audience; Diarrhea is a frequent complication of solid organ transplantation. Cryptosporidiosis is classically reported in patients with acquired immunodeficiency syndrome and emerged as a cause of persistent diarrhea in solid organ transplant patients.

Published
2017

124. Evaluation of a New Histoplasmaspp. Quantitative RT-PCR Assay

Author

Alanio, Alexandre, Gits-Muselli, Maud, Lanternier, Fanny, Sturny-Leclère, Aude, Benazra, Marion, Hamane, Samia, Rodrigues, Anderson Messias, Garcia-Hermoso, Dea, Lortholary, Olivier, Dromer, Françoise, Bretagne, Stéphane, Woimant, Marine Gosset, blanchard, Geneviève, Silhadi, Souad, Vignier, Nicolas, Pitsch, Aurelia, Jidar, Kaoutar, Traversier, Nicolas, Poisson, Didier, Lecointre, Claire, Foulet, Françoise, Botterel, Françoise, Ammar, Nawel Ait, Amsellem, Gabriel, Frederic, Poirier, Philipe, Cornu, Marjorie, Loridant, Severine, Morio, Florent, Boutoille, David, Jeddi, Fakhri, Hasseine, Lilia, Ouissa, Rachida, Toubas, Dominique, Bailly, Eric, Désoubeaux, Guillaume, Ronez, Emily, Foulon, Guillaume, Lefrançois, Sebastien, Bonnal, Christine, Paugam, André, Dougados, Maxime, Desroches, Marine, Barazzutti, Hélène, Paleiron, Nicolas, rabodonirina, Meja, Catherinot, Emilie, Cardot-Martin, Emilie, Hiesse, Chrisian, Salvator, Hélène, Aguilar, Claire, Gigandon, Anne, de Montpreville, Thomas, Bougnoux, Marie-Elisabeth, Sitterlé, Emilie, Fekkar, Arnaud, Imbert, Sébastien, Bleibtreu, Alexandre, Senghor, Yaye, Denis, Blandine, Molina, Jean-Michel, Liegeon, Geoffroy, Munnier, Anne-Lise, Malphettes, Marion, Denis, Julie, Berlioz-Arthaud, Alain, Lange, Franciska, Chiaruzzi, Myriam, and Epelboin, Loic

Abstract

Laboratory diagnosis of histoplasmosis is based on various methods, including microscopy, culture, antigen, and DNA detection of Histoplasma capsulatumvar. capsulatumor Histoplasma capsulatumvar. duboisii. To improve sensitivity of existing real-time quantitative PCR (qPCR) assays, we developed a new RT-qPCR assay that allows amplification of whole nucleic acids of Histoplasmaspp. validated on suspected cases. The limit of detection was 20 copies, and the specificity against 114 fungal isolates/species was restricted to Histoplasmaspp. Whole nucleic acids of 1319 prospectively collected consecutive samples from 907 patients suspected of having histoplasmosis were tested routinely between May 2015 and May 2019 in parallel with standard diagnostic procedures performed in parallel. Forty-four had proven histoplasmosis attributable to H. capsulatumvar. capsulatum(n= 40) or H. capsulatumvar. duboisii(n= 4) infections. The results of RT-qPCR were positive in 43 of 44 patients (97.7% sensitivity) in at least one specimen. Nine of 863 cases (99% specificity) were RT-qPCR positive and therefore classified as possible cases. RT-qPCR was positive in 13 of 30 (43.3%) blood samples tested in proven cases. A positive RT-qPCR result in blood was significantly associated with H. capsulatumvar. capsulatumprogressively disseminated histoplasmosis with a positive RT-qPCR result in 92.3% of the immunocompromised patients with disseminated disease. This new HistoplasmaRT-qPCR assay enabling amplification of H. capsulatumvar. capsulatumand H. capsulatumvar. duboisiiis highly sensitive and allows the diagnosis of histoplasmosis advantageously from blood and bronchoalveolar lavage fluid.

Published
2021
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125. Prenatal therapy with pyrimethamine + sulfadiazine vs spiramycin to reduce placental transmission of toxoplasmosis: a multicenter, randomized trial

Author

Mandelbrot, Laurent, primary, Kieffer, François, additional, Sitta, Rémi, additional, Laurichesse-Delmas, Hélène, additional, Winer, Norbert, additional, Mesnard, Louis, additional, Berrebi, Alain, additional, Le Bouar, Gwenaëlle, additional, Bory, Jean-Paul, additional, Cordier, Anne-Gaëlle, additional, Ville, Yves, additional, Perrotin, Franck, additional, Jouannic, Jean-Marie, additional, Biquard, Florence, additional, d’Ercole, Claude, additional, Houfflin-Debarge, Véronique, additional, Villena, Isabelle, additional, Thiébaut, Rodolphe, additional, Pons, Denis, additional, Nourrisson, C., additional, Lavergne, Rose-Anne, additional, Fillaux, Judith, additional, Assouline, Corinne, additional, Robert-Gangneux, Florence, additional, L’Ollivier, Coralie, additional, Bretelle, Florence, additional, Guidicelli, Béatrice, additional, Garcia, Patricia, additional, Cordier, Anne-Gaelle, additional, Benachi, Alexandra, additional, Vauloup-Fellous, Christelle, additional, Letamendia, Emmanuelle, additional, Bougnoux, Marie-Elisabeth, additional, Van Langendonck, Nathalie, additional, Potin, Jérôme, additional, Marty, Pierre, additional, Pomarès, Christelle, additional, Trastour, Cynthia, additional, Deleplancque, Anne Sophie, additional, Costa, Jean-Marc, additional, Chève, Marie-Thérèse, additional, Col, Jean-Yves, additional, Cimon, Bernard, additional, Sterkers, Y., additional, Lachaud, Laurence, additional, Burlet, Gilles, additional, Maréchaud, Martine, additional, Perraud, Estelle, additional, Grébille, Anne-Gaelle, additional, Valentin, Morgane, additional, Houzé, Sandrine, additional, Omnès, Sophie, additional, Chitrit, Yvon, additional, Boissinot, Christine, additional, Yéra, Hélène, additional, Anselem, Olivia, additional, Tsatsaris, Vassilis, additional, Sénat, Marie-Victoire, additional, Fuchs, Florent, additional, Angoulvant, Adela, additional, Muszynski, Charles, additional, Totet, Anne, additional, Noël, Catherine, additional, Bidat, Laurent, additional, Barjat, Tiphaine, additional, Flori, Pierre, additional, Pelloux, Hervé, additional, Brenier-Pinchart, Marie-Pierre, additional, Thong-Vanh, Catherine, additional, Mandelbrot, Laurent, additional, Floch, Corinne, additional, Carbillon, Lionel, additional, Lachassine, Eric, additional, Ricbourg, Aude, additional, Paris, Luc, additional, Dommergues, Marc, additional, Rousseau, Thierry, additional, Dalle, Frederic, additional, Dardé, Marie Laure, additional, Aubard, Véronique, additional, Olivier, Camille, additional, Verspyk, Eric, additional, and Favennec, Loic, additional

Published
2018
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126. Human cryptosporidiosis in immunodeficient patients in France (2015–2017)

Author

Costa, Damien, primary, Razakandrainibe, Romy, additional, Sautour, Marc, additional, Valot, Stéphane, additional, Basmaciyan, Louise, additional, Gargala, Gilles, additional, Lemeteil, Denis, additional, Favennec, Loïc, additional, Dalle, Frédéric, additional, Debourgogne, Anne, additional, Angoulvant, Adela, additional, Agnamey, Patrice, additional, bonhomme, Julie, additional, Degeilh, Brigitte, additional, Chemla, Cathy, additional, Garnaud, Cécile, additional, Lollivier, Coralie, additional, angebault, Cécile, additional, Delaunay, Pascal, additional, Desoubeaux, Guillaume, additional, Fréalle, Emilie, additional, Grenouillet, Frédéric, additional, Morio, Florent, additional, Botterel, Françoise, additional, Belkadi, Ghania, additional, Yera, Hélène, additional, Nevez, Gilles, additional, Iriart, Xavier, additional, Accoceberry, Isabelle, additional, Brunet, Julie, additional, Thellier, Marc, additional, Rabodonirina, Meja, additional, Sasso, Milene, additional, Miossec, Charline, additional, Nicolas, Murielle, additional, Desbois, Nicole, additional, Poirier, Philippe, additional, Pomares, Christelle, additional, Houze, Sandrine, additional, Le govic, Yohann, additional, Bellanger, Anne Pauline, additional, Labbe, Franck, additional, Darde, Marie Laure, additional, Duquesnoy, Olivier, additional, Flori, Pierre, additional, Bastien, Patrick, additional, Gueudet, Thomas, additional, Villena, Isabelle, additional, Aubert, Dominique, additional, Cateau, Estelle, additional, Bougnoux, Marie-Elisabeth, additional, Kapel, Nathalie, additional, Dannaoui, Eric, additional, Valentin, Alexis, additional, Totet, Anne, additional, Menotti, Jean, additional, Blanchet, Denis, additional, Demar, Magalie, additional, Dutoit, Emmanuel, additional, Piarroux, Renaud, additional, Sitterle, Emilie, additional, Magne, Denis, additional, Hamane, Samsa, additional, Berry, Antoine, additional, and Ramade, Cecile, additional

Published
2018
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127. Systematic gene overexpression inCandida albicansidentifies a regulator of early adaptation to the mammalian gut

Author

Znaidi, Sadri, primary, van Wijlick, Lasse, additional, Hernández-Cervantes, Arturo, additional, Sertour, Natacha, additional, Desseyn, Jean-Luc, additional, Vincent, Frédéric, additional, Atanassova, Ralitsa, additional, Gouyer, Valérie, additional, Munro, Carol A., additional, Bachellier-Bassi, Sophie, additional, Dalle, Frédéric, additional, Jouault, Thierry, additional, Bougnoux, Marie-Elisabeth, additional, and d'Enfert, Christophe, additional

Published
2018
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129. Gene flow contributes to diversification of the major fungal pathogen Candida albicans

Author

Ropars, Jeanne, primary, Maufrais, Corinne, additional, Diogo, Dorothée, additional, Marcet-Houben, Marina, additional, Perin, Aurélie, additional, Sertour, Natacha, additional, Mosca, Kevin, additional, Permal, Emmanuelle, additional, Laval, Guillaume, additional, Bouchier, Christiane, additional, Ma, Laurence, additional, Schwartz, Katja, additional, Voelz, Kerstin, additional, May, Robin C., additional, Poulain, Julie, additional, Battail, Christophe, additional, Wincker, Patrick, additional, Borman, Andrew M., additional, Chowdhary, Anuradha, additional, Fan, Shangrong, additional, Kim, Soo Hyun, additional, Le Pape, Patrice, additional, Romeo, Orazio, additional, Shin, Jong Hee, additional, Gabaldon, Toni, additional, Sherlock, Gavin, additional, Bougnoux, Marie-Elisabeth, additional, and d’Enfert, Christophe, additional

Published
2018
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130. Case of femoral pseudarthrosis due to Scedosporium apiospermum in an immunocompetent patient with successful conservative treatment and review of literature

Author

Dinh, Aurélien, primary, Demay, Olivier, additional, Rottman, Martin, additional, Gaudot, Fabrice, additional, Bougnoux, Marie‐Elisabeth, additional, Bouchand, Frédérique, additional, Coignard, Hélène, additional, Rouzaud, Claire, additional, Davido, Benjamin, additional, Duran, Clara, additional, and Nich, Christophe, additional

Published
2018
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133. Analyse du polymorphisme des gènes de Candida albicans impliqués dans la résistance aux azolés et aux échinocandines et description de potentielles nouvelles mutations de résistance aux azolés chez des isolats cliniques

Author

Sitterlé, Emilie, primary, Coste, Alix, additional, Maufrais, Corinne, additional, Sertour, Natacha, additional, Sanglard, Dominique, additional, D’enfert, Christophe, additional, and Bougnoux, Marie-Elisabeth, additional

Published
2017
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135. Analysis of Repair Mechanisms following an Induced Double-Strand Break Uncovers Recessive Deleterious Alleles in the Candida albicans Diploid Genome

Author

Feri, Adeline, primary, Loll-Krippleber, Raphaël, additional, Commere, Pierre-Henri, additional, Maufrais, Corinne, additional, Sertour, Natacha, additional, Schwartz, Katja, additional, Sherlock, Gavin, additional, Bougnoux, Marie-Elisabeth, additional, d’Enfert, Christophe, additional, and Legrand, Mélanie, additional

Published
2016
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136. Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

Author

Mouhajir, Abdelmounaim, primary, Matray, Olivier, additional, Giraud, Sandrine, additional, Mély, Laurent, additional, Marguet, Christophe, additional, Sermet-Gaudelus, Isabelle, additional, Le Gal, Solène, additional, Labbé, Franck, additional, Person, Christine, additional, Troussier, Françoise, additional, Ballet, Jean-Jacques, additional, Gargala, Gilles, additional, Zouhair, Rachid, additional, Bougnoux, Marie-Elisabeth, additional, Bouchara, Jean-Philippe, additional, and Favennec, Loïc, additional

Published
2016
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137. Micafungin susceptibility of the most common Candida species in 16 French university hospitals : comparison between the Etest® and the EUCAST methods (MICACAND study)

Author

Bougnoux, Marie-Elisabeth, Dannaoui, Eric, Accoceberry, Isabelle, Angoulevant, Adela, Bailly, E., Bouchara, Jean-Philippe, Botterel, Françoise, Chevrier, Sylviane, Chouaki, Taieb, Cornet, Muriel, Fekkar, Arnault, Dalle, Frédéric, Gangneux, Jean-Pierre, Guitard, Juliette, Hennequin, Christophe, Lepape, Patrice, Maubon, Danièle, Ranque, Stéphane, Sendid, Boualem, Chandenier, Jacques, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris], Unité de Parasitologie-Mycologie [CHU HEGP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Dynamic Microbiology - EA 7380 (DYNAMIC), École nationale vétérinaire - Alfort (ENVA)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), CHU de Bordeaux Pellegrin [Bordeaux], Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Hôpital Bretonneau, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM), Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), CHU Henri Mondor, CHU Pontchaillou [Rennes], CHU Amiens-Picardie, Université de Picardie Jules Verne (UPJV), CHU Grenoble, Service de Parasitologie - Mycologie [CHU Pitié-Salpétrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Hôpital du Bocage, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), CHU Saint-Antoine [AP-HP], Centre d'Immunologie et de Maladies Infectieuses (CIMI), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre hospitalier universitaire de Nantes (CHU Nantes), Cibles et médicaments de l'infection, de l'immunité et du cancer (IICiMed), Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes (UN)-Université de Nantes (UN), Assistance Publique - Hôpitaux de Marseille (APHM), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Centre Hospitalier Universitaire de Lille (CHU de Lille), Physiopathologie des Candidoses, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, Service de parasitologie, mycologie, médecine tropicale [CHRU Tours], European Congress of Clinical Microbiology and Infectious Deseases (ECCMID). INT., Biologie et Pathogénicité fongiques, Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Service de parasitologie - mycologie [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Microbiologie Fondamentale et Pathogénicité (MFP), CHU Henri Mondor [Créteil], Cibles et Médicaments des Infections et de l'Immunité (IICiMed), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)

Subjects
E-test, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, Micafungin, Candida, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, bacterial infections and mycoses
Abstract

EA MERS; International audience; Objectives : Micafungin is currently used in France. The aim of this study is to determine its activity against a recent (2014) French collection of Candida isolates. Although EUCAST is the reference method for in vitro antifungal susceptibility testing, it is not commonly used in routine clinical microbiology laboratories. Thus, it is important to evaluate alternative methods. We compared EUCAST and Etest for micafungin susceptibility testing of Candida spp. and we monitored the emergence of resistance. Methods: Sixteen centers (6 in Paris area and 10 across France) participated in a two-months prospective study. Clinical isolates of various Candida species (mainly C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, C. kefyr and C. krusei, about 10 isolates of each species per center) were tested by Etest, according to manufacturer’s instructions. All isolates were subsequently centralized in one center for MIC determination by EUCAST method. For comparison purposes, Etest MICs were raised to the next higher EUCAST concentration. Resistance was defined based on EUCAST clinical breakpoints or on epidemiological cut-off values when clinical breakpoints were not available. Results : A total number of 933 Candida isolates were tested. The overall agreement (+/- 2 log2 dilutions) between EUCAST and Etest was 97.9%. Species n E-Test EUCAST MIC range MIC range % agreement with Etest % resistance C. albicans 159 ≤ 0.015 - 0.06 ≤ 0.015 - 0.06 100 1.3 C. tropicalis 152 ≤ 0.015 - 0.5 ≤ 0.015 - 1 98.7 0.7 C. parapsilosis 152 ≤ 0.015 - 4 ≤ 0.125 - 4 96.1 1.3 C. glabrata 152 ≤ 0.015 - 0.125 ≤ 0.015 - 1 98.7 3.9 C. kefyr 136 ≤ 0.015 - 0.25 ≤ 0.015 - 0.125 97.8 ND C. krusei 127 ≤ 0.015 - 1 ≤ 0.015 - 0.25 96.9 0 Other Candida species* 55 ≤ 0.015 - 1 ≤ 0.015 - 1 94.5 ND Total 933 ≤ 0.015 - 4 ≤ 0.015 - 4 97.9 ND *: C. lusitaniae, C. guilliermondii, C. norvegensis, C. inconspicua, C. famata, C. pelliculosa, C. lambica, C. sphaerica, C. ciferii, C. catenulata, C. utilis, C. colliculosa, C. nivariensis Conclusions : This study demonstrated a very good agreement between Etest, performed on a routine basis, and EUCAST for micafungin MIC determination. Micafungin resistance among the main Candida species was uncommon.

Published
2015

138. Ecology of the different species of the Scedosporium apiospermum complex

Author

Giraud, Sandrine, Rougeron, Amandine, Mouhajir, A, Pham, Tram, Leto, Julie, Zouhair, Rachid, Bougnoux, Marie-Elisabeth, Bouchara, Jean-Philippe, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Université Moulay Ismail (UMI), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biologie et Pathogénicité fongiques, and Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

141. Unveiling Candida albicansintestinal carriage in healthy volunteers: the role of micro- and mycobiota, diet, host genetics and immune response

Author

Delavy, Margot, Sertour, Natacha, Patin, Etienne, Le Chatelier, Emmanuelle, Cole, Nathaniel, Dubois, Florian, Xie, Zixuan, Saint-André, Violaine, Manichanh, Chaysavanh, Walker, Alan W., Quintana-Murci, Lluis, Duffy, Darragh, d’Enfert, Christophe, Bougnoux, Marie-Elisabeth, and Consortium, Milieu Intérieur

Abstract

ABSTRACTCandida albicansis a commensal yeast present in the gut of most healthy individuals but with highly variable concentrations. However, little is known about the host factors that influence colonization densities. We investigated how microbiota, host lifestyle factors, and genetics could shape C. albicansintestinal carriage in 695 healthy individuals from the Milieu Intérieur cohort. C. albicansintestinal carriage was detected in 82.9% of the subjects using quantitative PCR. Using linear mixed models and multiway-ANOVA, we explored C. albicansintestinal levels with regard to gut microbiota composition and lifestyle factors including diet. By analyzing shotgun metagenomics data and C. albicansqPCR data, we showed that Intestinimonas butyriciproducenswas the only gut microbiota species whose relative abundance was negatively correlated with C. albicansconcentration. Diet is also linked to C. albicansgrowth, with eating between meals and a low-sodium diet being associated with higher C. albicanslevels. Furthermore, by Genome-Wide Association Study, we identified 26 single nucleotide polymorphisms suggestively associated with C. albicanscolonization. In addition, we found that the intestinal levels of C. albicansmight influence the host immune response, specifically in response to fungal challenge. We analyzed the transcriptional levels of 546 immune genes and the concentration of 13 cytokines after whole blood stimulation with C. albicanscells and showed positive associations between the extent of C. albicansintestinal levels and NLRP3expression, as well as secreted IL-2 and CXCL5 concentrations. Taken together, these findings open the way for potential new interventional strategies to curb C. albicansintestinal overgrowth.

Published
2023
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144. Human-impacted areas of France are environmental reservoirs of the Pseudallescheria boydii/Scedosporium apiospermum species complex

Author

Rougeron, Amandine, Schuliar, Gaëlle, Leto, Julie, Sitterlé, Emilie, Landry, David, Bougnoux, Marie-Elisabeth, Kobi, Abdessamad, Bouchara, Jean-Philippe, Giraud, Sandrine, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Littoral, Environnement, Télédétection, Géomatique UMR 6554 (LETG), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université d'Angers (UA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Brest (UBO)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Géographie et d'Aménagement Régional de l'Université de Nantes (IGARUN), Université de Nantes (UN)-Université de Nantes (UN), and Laboratoire Angevin de Recherche en Ingénierie des Systèmes (LARIS)

Subjects
Cystic Fibrosis, education, Wastewater, Pseudallescheria, Industrial Microbiology, Mycoses, [SDE]Environmental Sciences, Humans, France, Scedosporium, DNA, Fungal, Lung, Soil Microbiology, Disease Reservoirs
Abstract

International audience; : Species of the Pseudallescheria boydii/Scedosporium apiospermum complex (PSC) are emerging fungal pathogens able to chronically colonize the airways of patients with cystic fibrosis (CF). As P. boydii was found more frequently colonizing the lungs of CF patients in France than in other European countries in a previous report, the present study was conducted in order to clarify distribution of PSC species in France and to characterize their natural habitat. The highest densities of PSC isolates were found in human-impacted areas, i.e. agricultural areas, fluids obtained from wastewater treatment plants, playgrounds and industrial areas. PSC was not detected from soil samples collected in forests. Most PSC culture-positive soil samples exhibited a pH range of 6-8. Scedosporium dehoogii, the most abundant species, was detected in all human-impacted area types except vineyards, whereas Scedosporium aurantiacum was mostly found in agricultural areas. Pseudallescheria boydii and S. apiospermum were predominantly isolated from seashores and playgrounds respectively. Pseudallescheria minutispora was found only once from a playground. This study highlights potential sources of contamination of the patients, especially in the CF context.

Published
2014

145. Sequential variations of Rasamsonia spp. isolate genotypes in cystic fibrosis patients

Author

Matray, Olivier, Mouhajir, A, Giraud, Sandrine, Gargala, Gilles, Zouhair, Rachid, Bougnoux, Marie-Elisabeth, Bouchara, Jean-Philippe, Favennec, Loïc, Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA), Hôpital Charles Nicolle [Rouen], Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Université Moulay Ismail (UMI), Biologie et Pathogénicité fongiques, Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Appareil Digestif Environnement Nutrition (ADEN ), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Université de Reims Champagne-Ardenne (URCA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), CHU Rouen, Biologie et Pathogénicité fongiques (BPF), and Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

International audience; Rasamsonia species have been recently described as emergent pathogens in human and animals. Recent multilocus phylogenetic studies have shown that the genus Rasamsonia (R.) includes at least four phylogenetically related species forming the R. argillacea complex, R. argillacea sensu stricto, R. piperina, R. eburnea and R. aegroticola, which have been mostly reported in the clinical context of chronic granulomatous disease (CGD) and cystic fibrosis (CF). Differences in sensitivity to antifungal therapies which have been noted within the genus Rasamsonia underline the importance of early identification of clinical isolates for optimal treatment during the early stages of infection. In this context, the aim of this work was to obtain genotypic characterization of clinical Rasamsonia spp. isolates from 26 CF patients and one acutely infected CGD patient using PCR amplification of repetitive DNA sequences (rep-PCR) and to compare species distributions determined by β tubulin sequencing with rep-PCR genotypes/clusters. One hundred and ten isolates were collected sequentially or simultaneously from 01/09/1998 to 08/2012 from sputum samples of 26 CF patients. One CGD patient (4 isolates) was studied in parallel. Two references strains (CBS #408.73 (R. piperina) and #432.62 (R. cylindrospora) were obtained from CBS. After extraction, PCR amplifications of repetitive DNA sequences were realized for each sample using the DiversiLab Fungal kit (bioMérieux). Amplicons were separated by capillary electrophoresis in an Agilent 2100 Bioanalyzer (Massy, France). Single electrophoretic profiles obtained for each isolate were compared using the DiversiLab Healthcare software (version 3.41). As previously reported in CF patients, beta-tubulin gene sequencing of 110 isolates identified more frequently R. aegroticola (72 isolates from 19 CF patients) than R. argillacea stricto sensu (30 isolates from 8 CF patients), and R. piperina (8 isolates from 5 CF patients), with 15, 6, and 7 distinct profiles, respectively. From the CGD patient, all isolates were identified as R. argillacea stricto sensu. Without exception, species identification according to rep-PCR genotypes was entirely consistent with beta-tubulin sequencing results. Present data provide the first description of sequential Rasamsonia spp. variations with time in CF patients. Rasamsonia argillacea sensu stricto isolates were found persistent for years in all sequentially sampled CF patients except 1 who presented 4 different successive genotypes. In contrast to R. argillacea sensu stricto, sequential detection of R. aegroticola and R. piperina revealed frequent successive changes in isolate genotypes in most CF patients.

Published
2014

146. Ecology of the Scedosporium apiospermum species complex and species distribution in Morocco

Author

Mouhajir, A, Zouhair, Rachid, Angebault, Cécile, Jacob, S., Giraud, Sandrine, Bouabid, R., Bougnoux, Marie-Elisabeth, Bouchara, Jean-Philippe, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Université Moulay Ismail (UMI), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5), Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP), Biologie et Pathogénicité fongiques, and Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2014

147. Rasamsonia species isolated from cystic fibrosis patients: MALDI-TOF/MS identification and antifungal susceptibility by EUCAST method

Author

Giraud, Sandrine, Dannaoui, Eric, Leto, Julie, Mouhajir, A, Angebault, Cécile, Béretti, Jean-Luc, Favennec, Loïc, Bouchara, Jean-Philippe, Bougnoux, Marie-Elisabeth, Jacob, S., Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Unité de Parasitologie-Mycologie [CHU HEGP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Dynamic Microbiology - EA 7380 (DYNAMIC), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5), Appareil Digestif Environnement Nutrition (ADEN ), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris], Univ Angers, Okina, École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Biologie et Pathogénicité fongiques, and Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Abstract

International audience; A wide variety of filamentous fungi are able to colonize respiratory tract of patients with cystic fibrosis (CF). Recently, fungi of the genus Rasamsonia (formely Geosmithia) have been described as a new emergent group of fungi pathogen in CF patients. The genus Rasamsonia comprises nine species including Rasamsonia argillacea (sensu lato) which is a complex of species (R. argillacea sensu stricto, R. piperina , R. eburnea, and R. aegroticola). Only species belonging to species complex R. argillacea have been yet isolated from patients with chronic granulomatous disease and CF. Accurate identification at the species level of the members of the Rasamsonia complex on the basis of morphology criteria is challenging, and frequent misidentification with a Penicillium emersonii have been reported.Matrix-assisted laser desorption ionization (MALDI)-time of flight (TOF)/mass spectrometry (MS) is a powerful tool to rapidly identify moulds at the species level. We investigated the potential of this technology to discriminate Rasamsonia species. Nine reference strains were used to build a reference database library. Profiles from 3-, 5- and 7-day-old cultures of each reference strain were analysed to identify species-specific discriminating profiles. The database was tested for accuracy using a set of 74 clinical isolates collected from 29 CF-patients in 4 French hospitals (Rouen, Giens, Angers and Paris). For each isolate, the species identification obtained using MALDI-TOF/MS was compared to this obtained by Diversilab method. Our results showed a high reliability of the MALDI-TOF analysis.Antifungal susceptibility testing was also performed for 20 isolates (identified as R. argillacea, R. aegroticola or R. piperina) from 13 patients using the microdilution broth reference method of the Antifungal Susceptibility Testing Subcommittee of EUCAST. All isolates tested showed a high MIC of > 8 mg/L to voriconazole, and the majority also showed high MICs to itraconazole (MIC > 8 mg/L), except for R. piperina strains (MIC 0.5 - 1 mg/L). The susceptibility to amphotericin B was variable (MIC: 0.5 - > 8 mg/L), with no specific distinction according to Rasamsonia species. Finally, all isolates exhibited low MICs to micafungin (0.0312 - 0.125 mg/L).Altogether, our results show that MALDI-TOF/MS is a powerful tool for rapid identification of Rasamsonia species that cannot be currently identified by morphological examination in the clinical setting, and confirm that the members of R. argillacea complex, colonizing respiratory tract in CF patients, have a very low susceptibility to available antifungal agents.

Published
2014

148. A study of the DNA damage checkpoint in Candida albicans: uncoupling of the functions of Rad53 in DNA repair, cell cycle regulation and genotoxic stress-induced polarized growth

Author

Loll-Krippleber, Raphaël, D'Enfert, Christophe, Feri, Adeline, Diogo, Dorothée, Perin, Aurélie, Marcet-Houben, Marina, Bougnoux, Marie-Elisabeth, Legrand, Mélanie, Cellule Pasteur, Université Paris Diderot - Paris 7 (UPD7)-PRES Sorbonne Paris Cité, Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP), We thank Frank Feuerbach for kindly providing S. cerevisiae strains and Ute Zeidler and Sadri Znaïdi for constructing thepUC18-SAT1 and CIp-PTDH3-GTW plasmid. Thanks are due to Guy-Franck Richard, Guilhem Janbon and Carol Munro forinsights in the manuscript and useful comments. We also thank Marie N’Guyen-de Bernon from the Pasteur Imagopolefor her help with cell sorting and Christiane Bouchier and Laurence Ma from the Pasteur Plateforme Genomique (PF1) for Illumina sequencing. We are grateful to other members of Fungal Biology and Pathogenicity Unit for helpful discussions. This study has received funding from the French Government’s Investissement d’Avenir programme, Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (Grant # ANR-10-LABX-62-IBEID). R.L.-K. and D.D.were the recipients of PhD grants from INRA and Institut Pasteur, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Biologie et Pathogénicité fongiques, Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris], and ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010)

Subjects
AZOLE RESISTANCE, MESH: Sequence Analysis, DNA, GENES, DNA Repair, Loss of Heterozygosity, PROTEIN, MESH: Cell Cycle, MESH: Checkpoint Kinase 2, Fungal Proteins, SACCHAROMYCES-CEREVISIAE, S-PHASE CHECKPOINT, Gene Expression Regulation, Fungal, MESH: Mutagens, Candida albicans, KINASE, MESH: Aneuploidy, RIBONUCLEOTIDE REDUCTASE INHIBITOR, PHOSPHORYLATION, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, MESH: DNA Damage, MESH: DNA Repair, MESH: Loss of Heterozygosity, MESH: Polymorphism, Restriction Fragment Length, MESH: Candida albicans, Cell Cycle, GENOME STABILITY, Sequence Analysis, DNA, Aneuploidy, CHROMOSOME LOSS, Checkpoint Kinase 2, MESH: Mutagenesis, Site-Directed, MESH: Gene Deletion, Mutagenesis, Site-Directed, MESH: Fungal Proteins, Gene Deletion, Polymorphism, Restriction Fragment Length, MESH: Gene Expression Regulation, Fungal, DNA Damage, Mutagens
Abstract

International audience; In response to genotoxic stress (GS), Candida albicans can undergo polarized growth and massive genome rearrangements including loss-of-heterozygosity (LOH) events. We evaluated the contribution of the CaRad53p and CaDun1p kinases of the DNA damage checkpoint (DDCP) in these processes. Characterization of C. albicans rad53ΔΔ and dun1ΔΔ mutants revealed that the two kinases were involved in the maintenance of heterozygosity. SNP-RFLP typing and whole-genome sequencing of rad53ΔΔ isolates having undergone a LOH revealed that, according to the chromosome on which LOH had occurred, these were predominantly due to break-induced replication/mitotic cross-over or chromosome loss. Loss of CaRAD53 also resulted in frequent aneuploidies. Deletion of CaDUN1 led to an increase in recombination-dependent LOH but did not trigger aneuploidies. It also increased GS sensitivity but did not impair GS-induced polarized growth contrary to CaRAD53 deletion. Characterization of CaRad53p site-directed mutants demonstrated that its kinase activity and N-terminal phosphorylation sites were crucial for its function in the resistance to GS, maintenance of heterozygosity, cell cycle regulation and polarized growth. Moreover, using phosphomimic mutants, we revealed an uncoupling of the functions of CaRad53p in these different processes, thus providing a novel understanding of how the DDCP may regulate downstream events in response to GS.

Published
2013

150. Comparison of E,E-Farnesol Secretion and the Clinical Characteristics of Candida albicans Bloodstream Isolates from Different Multilocus Sequence Typing Clades

Author

Jung, Sook-In, primary, Shin, Jong Hee, additional, Kim, Soo Hyun, additional, Kim, Jin, additional, Kim, Joo Hee, additional, Choi, Min Ji, additional, Chung, Eun-Kyung, additional, Lee, Kyungwon, additional, Koo, Sun Hoe, additional, Chang, Hyun Ha, additional, Bougnoux, Marie-Elisabeth, additional, and d’Enfert, Christophe, additional

Published
2016
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