133 results on '"Bhide, Pradeep"'
Search Results
102. The Sequence of Formation and Development of Corticostriate Connections in Mice
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Sheth, Ami N., primary, McKee, Mary L., additional, and Bhide, Pradeep G., additional
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- 1998
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103. Concurrent cellular output from two proliferative populations in the early embryonic mouse corpus striatum
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Sheth, Ami N., primary and Bhide, Pradeep G., additional
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- 1997
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104. Cell cycle kinetics in the embryonic mouse corpus striatum
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Bhide, Pradeep G., primary
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- 1996
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105. Expression of Normal and Mutant Huntingtin in the Developing Brain
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Bhide, Pradeep G., primary, Day, Michelle, additional, Sapp, Ellen, additional, Schwarz, Cordula, additional, Sheth, Ami, additional, Kim, Johnny, additional, Young, Anne B., additional, Penney, John, additional, Golden, Jeffrey, additional, Aronin, Neil, additional, and DiFiglia, Marian, additional
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- 1996
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106. Neuronal high-affinity glutamate transport in the rat central nervous system
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Kanai, Yoshikatsu, primary, Bhide, Pradeep G., additional, DiFiglia, Marian, additional, and Hediger, Matthias A., additional
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- 1995
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107. Transient Axonal Branching in the Developing Corpus Callosum
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Kadhim, Hazim J., primary, Bhide, Pradeep G., additional, and Frost, Douglas O., additional
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- 1993
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108. Prenatal Nicotine Exposure Mouse Model Showing Hyperactivity, Reduced Cingulate Cortex Volume, Reduced Dopamine Turnover, and Responsiveness to Oral Methylphenidate Treatment.
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Jinmin Zhu, Xuan Zhang, Yuehang Xu, Spencer, Thomas J., Biederman, Joseph, and Bhide, Pradeep G.
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PREGNANT women ,WOMEN'S tobacco use ,METHYLPHENIDATE ,NICOTINE ,DOPAMINE ,NICOTINE replacement therapy ,BIOMARKERS ,LABORATORY mice - Abstract
Cigarette smoking, nicotine replacement therapy, and smokeless tobacco use during pregnancy are associated with cognitive disabilities later in life in children exposed prenatally to nicotine. The disabilities include attention deficit hyperactivity disorder (ADHD) and conduct disorder. However, the structural and neurochemical bases of these cognitive deficits remain unclear. Using a mouse model we show that prenatal nicotine exposure produces hyperactivity, selective decreases in cingulate cortical volume, and radial thickness, as well as decreased dopamine turnover in the frontal cortex. The hyperactivity occurs in both male and female offspring and peaks during the "active" or dark phase of the light/dark cycle. These features of the mouse model closely parallel the human ADHD phenotype, whether or not the ADHD is associated with prenatal nicotine exposure. A single oral, but not intraperitoneal, administration of a therapeutic equivalent dose (0.75 mg/kg) of methylphenidate decreases the hyperactivity and increases the dopamine turnover in the frontal cortex of the prenatally nicotine exposed mice, once again paralleling the therapeutic effects of this compound in ADHD subjects. Collectively, our data suggest that the prenatal nicotine exposure mouse model has striking parallels to the ADHD phenotype not only in behavioral, neuroanatomical, and neurochemical features, but also with respect to responsiveness of the behavioral phenotype to methylphenidate treatment. The behavioral, neurochemical, and anatomical biomarkers in the mouse model could be valuable for evaluating new therapies for ADHD and mechanistic investigations into its etiology. [ABSTRACT FROM AUTHOR]
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- 2012
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109. Does exposure to maternal smoking during pregnancy affect the clinical features of ADHD? Results from a controlled study.
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Biederman, Joseph, Petty, Carter R, Bhide, Pradeep G, Woodworth, K. Yvonne, and Faraone, Stephen
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MEDICAL research ,PREGNANT women ,WOMEN'S tobacco use ,RISK factors of attention-deficit hyperactivity disorder ,BEHAVIOR disorders in children ,CHILD development - Abstract
Objectives. Exposure to maternal smoking during pregnancy may be a significant risk factor for attention-deficit/hyperactivity disorder (ADHD) independently of family history of ADHD. The main aim of this study was to examine whether the clinical profile of ADHD differs between children with and without exposure to maternal smoking during pregnancy. Methods. This was a case-control study of boys and girls with and without ADHD ascertained from psychiatric and paediatric sources. Maternal smoking during pregnancy was defined by interviews with subjects' mothers. Main outcome measures were ADHD symptoms and associated clinical features in children with and without exposure to maternal smoking during pregnancy. Results. No significant differences were found between ADHD children with and without exposure to maternal smoking during pregnancy on clinical characteristics. When these analyses were repeated in the subgroup of subjects without parental history of ADHD, there were also no statistically significant differences found. Conclusions. Despite adequate statistical power, no significant differences were found between ADHD children with and without exposure in the clinical features of ADHD and associated disorders. Results provide support for the notion that ADHD cases resulting from exposure to maternal smoking during pregnancy have similar clinical profiles as other ADHD cases. [ABSTRACT FROM AUTHOR]
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- 2012
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110. Cocaine Alters BDNF Expression and Neuronal Migration in the Embryonic Mouse Forebrain.
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McCarthy, Deirdre M., Xuan Zhang, Darnell, Shayna B., Sangrey, Gavin R., Yanagawa, Yuchio, Sadri-Vakili, Ghazaleh, and Bhide, Pradeep G.
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COCAINE ,DRUG use in pregnancy ,CELL migration ,PROSENCEPHALON ,LABORATORY mice ,NEURAL development ,COGNITION in children ,CHILD development ,GENE expression - Abstract
The article focuses on a study conducted on mice to examine the impact of prenatal cocaine exposure on child's brain development and cognitive functions. The study found that daily exposure to cocaine during prenatal period may reduce tangential and radial migration of neurons in forebrain. The study further revealed that cocaine may also result in the modification of brain-derived neurotrophic factor (BDNF) expression. Several graphs related to the study are also presented.
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- 2011
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111. DNA-Based MRI Probes for Specific Detection of Chronic Exposure to Amphetamine in Living Brains.
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Liu, Christina H., Ren, Jia Q., Jinsheng Yang, Charng-ming Liu, Mandeville, Joseph B., Rosen, Bruce R., Bhide, Pradeep G., Yanagawa, Yuchio, and Liu, Philip K.
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DNA ,MAGNETIC resonance imaging ,NERVOUS system ,AMPHETAMINES ,DNA probes ,MAGNETIC fields ,IRON oxides ,MICE - Abstract
We designed phosphorothioate-modified DNA probes linked to superparamagnetic iron oxide nanoparticles (SPION) for in vivo magnetic resonance imaging (MRI) of fosB and ΔfosB mRNA after amphetamine (AMPH) exposure in mice. Specificity of both the fosB and ΔfosB probes was verified by in vitro reverse transcriptase-PCR amplification to a single fragment of total cDNA obtained from acutely AMPH-exposed mouse brains. We confirmed time-dependent uptake and retention profiles of both probes in neurons of GAD67-green fluorescent protein knock-in mice. MRI signal of SPION-labeled fosB probe delivered via intracerebroventricular route was elevated in both acutely and chronically AMPH-exposed mice; the signal was suppressed by dopaminergic receptor antagonist pretreatment. SPIONlabeled ΔfosB probe signal elevation occurred only in chronically AMPH-exposed mice. The in vivo target specificity of these probes permits reliable MRI visualization of AMPH-induced differential elevations of fosB and ΔfosB mRNA in living brains. [ABSTRACT FROM AUTHOR]
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- 2009
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112. Modes and Mishaps of Neuronal Migration in the Mammalian Brain.
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Métin, Christine, Vallee, Richard B., Rakic, Pasko, and Bhide, Pradeep G.
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NEURONS ,NEURAL development ,NEUROPLASTICITY ,HUMAN migration patterns ,BRAIN diseases ,MEDICAL genetics - Abstract
The ability of neurons to migrate to their appropriate positions in the developing brain is critical to brain architecture and function. Recent research has elucidated different modes of neuronal migration and the involvement of a host of signaling factors in orchestrating the migration, as well as vulnerabilities of this process to environmental and genetic factors. Here we discuss the role of cytoskeleton, motor proteins, and mechanisms of nuclear translocation in radial and tangential migration of neurons. We will also discuss how these and other events essential for normal migration of neurons can be disrupted by genetic and environmental factors that contribute to neurological disease in humans. [ABSTRACT FROM AUTHOR]
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- 2008
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113. Navigating Neocortical Neurogenesis and Neuronal Specification: A Positional Information System Encoded by Neurogenetic Gradients.
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Suter, Bernhard, Nowakowski, Richard S., Bhide, Pradeep G., and Caviness, Verne S.
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NEOCORTEX ,DEVELOPMENTAL neurobiology ,NEUROGENETICS ,NEURONS ,CELL cycle - Abstract
The projection neurons of the neocortex are produced in the pseudostratified ventricular epithelium (PVE) lining the embryonic lateral ventricles. Over a 7 d period in mouse, these neurons arise in an overlapping layer VI-to-II sequence and in an anterolateral to posteromedial gradient [the transverse neurogenetic gradient (TNG)]. At any time in the 7 d neurogenetic interval, a given PVE cell must know what class of precursor cell or neuron to form next. How this information is encoded in the PVE is not known. With comparative experiments in wild-type and double-transgenic mice, overexpressing the cell cycle inhibitor p27
Kip1 , we show that a gradient of expression of Lhx2 (inferred from its mRNA levels), a LIM homeodomain transcription factor, together with a gradient in duration of the G1 phase of the cell cycle (TG1 ), are sufficient to specify a positional mapping system that informs the PVE cell what class of neuron to produce next. Lhx2 likely is representative of an entire class of transcription factors expressed along the TNG. This mapping system consisting of a combination of signals from two different sources is a novel perspective on the source of positional information for neuronal specification in the developing CNS. [ABSTRACT FROM AUTHOR]- Published
- 2007
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114. Involvement of Matrix Metalloproteinase in Neuroblast Cell Migration from the Subventricular Zone after Stroke.
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Seong-Ryong Lee, Hahn-Young Kim, Rogowska, Jadwiga, Bing-Qiao Zhao, Bhide, Pradeep, Parent, Jack M., and Lo, Eng H.
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BRAIN injuries ,NEURONS ,BROMODEOXYURIDINE ,EXTRACELLULAR matrix proteins ,METALLOPROTEINASES ,TISSUES - Abstract
After brain injury, neuroblast cells from the subventricular zone (SVZ) expand and migrate toward damaged tissue. The mechanisms that mediate these neurogenic and migratory responses remain to be fully dissected. Here, we show that bromodeoxyuridine-labeled and doublecortin-positive cells from the SVZ colocalize with the extracellular protease matrix metalloproteinase-9 (MMP-9) during the 2 week recovery period after transient focal cerebral ischemia in mice. Treatment with the broad spectrum MMP inhibitor GM6001 significantly decreases the migration of doublecortin-positive cells that extend from the SVZ into the striatum. These data suggest that MMPs are involved in endogenous mechanisms of neurogenic migration as the brain seeks to heal itself after injury. [ABSTRACT FROM AUTHOR]
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- 2006
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115. Neuronal highaffinity glutamate transport in the rat central nervous system
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Kanai, Yoshikatsu, Bhide, Pradeep G., DiFiglia, Marian, and Hediger, Matthias A.
- Abstract
EAAC1 is a neuronal and epithelial high affinity glutamate transporter previously cloned from rabbit intestine. Here we report the isolation of EAAC 1 from rat brainand its expression in the central nervous system based on in situhybridization. Strong signals were detected in brain, spinal cord and retina. Expression of EAAC1 was particularly strong in pyramidal cells of the cerebral cortex, pyramidal cells of the hippocampus, mitral cells of the olfactory bulb, various thalamic nuclei and cells of certain retinal layers. EAAC1 was also expressed in non-glutamatergic neurons such as GABAergic cerebellar Purkinje cells and α-motor neurons of the spinal cord. We propose that EAAC1 is not only involved in the sequestration of glutamate at glutamatergic synapses and in protecting neurons from glutamate excitotoxicity, but also in the cellular metabolism involving glutamate.
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- 1995
116. Nicotine and the developing brain: Insights from preclinical models.
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McCarthy, Deirdre M., Zhang, Lin, Wilkes, Bradley J., Vaillancourt, David E., Biederman, Joseph, and Bhide, Pradeep G.
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ANIMAL models in research , *ATTENTION-deficit hyperactivity disorder , *NICOTINE , *BRAIN injuries , *TOBACCO products - Abstract
Use of tobacco products during pregnancy is associated with increased risk for neurodevelopmental disorders in the offspring. Preclinical models of developmental nicotine exposure have offered valuable insights into the neurobiology of nicotine's effects on the developing brain and demonstrated lasting effects of developmental nicotine exposure on brain structure, neurotransmitter signaling and behavior. These models have facilitated discovery of novel compounds as candidate treatments for attention deficit hyperactivity disorder, a neurodevelopmental disorder associated with prenatal nicotine exposure. Using these models the significance of heritability of behavioral phenotypes from the nicotine-exposed pregnant female or adult male to multiple generations of descendants has been demonstrated. Finally, research using the preclinical models has demonstrated synergistic interactions between developmental nicotine exposure and repetitive mild traumatic brain injury that contribute to "worse" outcomes from the injury in individuals with attention deficit hyperactivity disorder associated with developmental nicotine exposure. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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117. The E3 Ubiquitin Ligase Protein Associated with Myc (Pam) Regulates Mammalian/Mechanistic Target of Rapamycin Complex 1 (mTORC1) Signaling in Vivo through N- and C-terminal Domains.
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Sangyeul Han, Sun Kim, Bahl, Samira, Lin Li, Burande, Clara F., Smith, Nicole, James, Marianne, Beauchamp, Roberta L., Bhide, Pradeep, DiAntonio, Aaron, and Ramesh, Vijaya
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UBIQUITIN ligases , *MYC proteins , *RAPAMYCIN , *IN vivo toxicity testing , *MAMMAL growth , *IMMUNOSUPPRESSIVE agents - Abstract
Pam and its homologs (the PHR protein family) are large E3 ubiquitin ligases that function to regulate synapse formation and growth in mammals, zebrafish, Drosophila, and Caenorhabditis elegans. Phr1-deficient mouse models (Phr1Δ8,9 and Phr1Magellan, with deletions in the N-terminal putative guanine exchange factor region and the C-terminal ubiquitin ligase region, respectively) exhibit axon guidance/outgrowth defects and striking defects of major axon tracts in the CNS. Our earlier studies identified Pam to be associated with tuberous sclerosis complex (TSC) proteins, ubiquitinating TSC2 and regulating mammalian/mechanistic target of rapamycin (mTOR) signaling. Here, we examine the potential involvement of the TSC/ mTOR complex 1(mTORC1) signaling pathway in Phr1-deficient mouse models. We observed attenuation of mTORC1 signaling in the brains of both Phr1Δ8,9 and Phr1Magellan mouse models. Our results establish that Pam regulates TSC/mTOR signaling in vitro and in vivo through two distinct domains. To further address whether Pam regulates mTORC1 through two functionally independent domains, we undertook heterozygous mutant crossing between Phr1Δ8,9 and Phr1Magellan mice to generate a compound heterozygous model to determine whether these two domains can complement each other. mTORC1 signaling was not attenuated in the brains of double mutants (Phr1Δ8,9/Mag), confirming that Pam displays dual regulation of the mTORC1 pathway through two functional domains. Our results also suggest that although dysregulation of mTORC1 signaling may be responsible for the corpus callosum defects, other neuro-developmental defects observed with Phr1 deficiency are independent of mTORC1 signaling. The ubiquitin ligase complex containing Pam-Fbxo45 likely targets additional synaptic and axonal proteins, which may explain the overlapping neuro-developmental defects observed in Phr1 and Fbxo45 deficiency. [ABSTRACT FROM AUTHOR]
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- 2012
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118. Overexpression of p27[sup Kip1] lengthens the G[sub 1] phase in a mouse model that targets...
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Mitsuhashi, Takayuki, Aoki, Yoko, Eksioglu, Yaman Z., Takahashi, Takao, Bhide, Pradeep G., Reeves, Steven A., and Caviness Jr., Vernes S.
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GENE expression , *EPITHELIUM , *CENTRAL nervous system - Abstract
Describes a mouse model in which p27[sup Kip1] transgene expression is spatially restricted to the central nervous system (CNS) neuroepithelium and temporally controlled with doxycycline (dox). Targeting dox-inducible transgene expression to the CNS neuroepithelium; Dox dosage and administration for optimal in vivo transgene expression.
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- 2001
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119. Behavioral, neurotransmitter and transcriptomic analyses in male and female Fmr1 KO mice.
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McCarthy DM, Vied C, Trupiano MX, Canekeratne AJ, Wang Y, Schatschneider C, and Bhide PG
- Abstract
Introduction: Fragile X syndrome is an inherited X-linked disorder associated with intellectual disabilities that begin in childhood and last a lifetime. The symptoms overlap with autism spectrum disorder, and the syndrome predominantly affects males. Consequently, FXS research tends to favor analysis of social behaviors in males, leaving a gap in our understanding of other behavioral traits, especially in females., Methods: We used a mouse model of FXS to analyze developmental, behavioral, neurochemical, and transcriptomic profiles in males and females., Results: Our behavioral assays demonstrated locomotor hyperactivity, motor impulsivity, increased "approach" behavior in an approach-avoidance assay, and deficits in nest building behavior. Analysis of brain neurotransmitter content revealed deficits in striatal GABA, glutamate, and serotonin content. RNA sequencing of the ventral striatum unveiled expression changes associated with neurotransmission as well as motivation and substance use pathways. Sex differences were identified in nest building behavior, striatal neurotransmitter content, and ventral striatal gene expression., Discussion: In summary, our study identified sex differences in specific behavioral, neurotransmitter, and gene expression phenotypes and gene set enrichment analysis identified significant enrichment of pathways associated with motivation and drug reward., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 McCarthy, Vied, Trupiano, Canekeratne, Wang, Schatschneider and Bhide.)
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- 2024
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120. Metabolic engineering improves transduction efficiency and downstream vector isolation by altering the lipid composition of extracellular vesicle-enclosed AAV.
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Espinoza P, Cheng M, Ng C, De La Cruz D, Wasson ED, McCarthy DM, Bhide PG, Maguire CA, and Santoscoy MC
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Adeno-associated viruses (AAV) are promising vectors for gene therapy due to their efficacy in vivo. However, there is room for improvement to address key limitations such as the pre-existing immunity to AAV in patients, high-dose toxicity, and relatively low efficiency for some cell types. This study introduces a metabolic engineering approach, using knockout of the enzyme phosphatidylserine synthase 1 (PTDSS1) to increase the abundance of extracellular vesicle-enclosed AAV (EV-AAV) relative to free AAV in the supernatant of producer cells, simplifying downstream purification processes. The lipid-engineered HEK293T-ΔPTDSS1 cell line achieved a 42.7-fold enrichment of EV-AAV9 compared to free AAV9 in the supernatant. The rational genetic strategy also led to a 300-fold decrease of free AAV in supernatant compared to wild-type HEK293T. The membrane-engineered EV-AAV9 (mEV-AAV9) showed unique envelope composition alterations, including cholesterol enrichment and improved transduction efficiency in human AC16 cardiomyocytes by 1.5-fold compared to conventional EV-AAV9 and by 11-fold compared to non-enveloped AAV9. Robust in-vivo transduction four weeks after intraparenchymal administration of mEV-AAV9 was observed in the murine brain. This study shows promise in the potential of lipid metabolic engineering strategies to improve the efficiency and process development of enveloped gene delivery vectors. Keywords: membrane engineering, EV-AAV, upstream, downstream.
- Published
- 2024
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121. Preclinical Models of Attention Deficit Hyperactivity Disorder: Neurobiology, Drug Discovery, and Beyond.
- Author
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McCarthy DM, Spencer TJ, and Bhide PG
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- Pregnancy, Mice, Animals, Female, Humans, Nicotine therapeutic use, Neurobiology, Mice, Inbred C57BL, Drug Discovery, Attention Deficit Disorder with Hyperactivity drug therapy, Methylphenidate pharmacology, Methylphenidate therapeutic use, Central Nervous System Stimulants pharmacology, Central Nervous System Stimulants therapeutic use
- Abstract
Objective: We offer an overview of ADHD research using mouse models of nicotine exposure., Method: Nicotine exposure of C57BL/6 or Swiss Webster mice occurred during prenatal period only or during the prenatal and the pre-weaning periods. Behavioral, neuroanatomical and neurotransmitter assays were used to investigate neurobiological mechanisms of ADHD and discover candidate ADHD medications., Results: Our studies show that norbinaltorphimine, a selective kappa opioid receptor antagonist is a candidate novel non-stimulant ADHD treatment and that a combination of methylphenidate and naltrexone has abuse deterrent potential with therapeutic benefits for ADHD. Other studies showed transgenerational transmission of ADHD-associated behavioral traits and demonstrated that interactions between untreated ADHD and repeated mild traumatic brain injury produced behavioral traits not associated with either condition alone., Conclusion: Preclinical models contribute to novel insights into ADHD neurobiology and are valuable tools for drug discovery and translation to benefit humans with ADHD., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: Deirdre McCarthy and Pradeep Bhide are co-founders of Avekshan, LLC, a pharmaceutical company located in Tallahassee, FL. Dr. Thomas Spencer has, in the last two years, received support from Royalties and Licensing fees on copyrighted ADHD scales through MGH Corporate Sponsored Research and Licensing. Drs. Spencer and Bhide are co-inventors on US Patent US 9.623,023 B2 for a non-stimulant treatment for ADHD (no license fees) and US 11,045,465 B2 for a method to prevent stimulant abuse (no license fees). Dr. Thomas Spencer receives publication royalties from Cambridge University Press.
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- 2024
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122. Learning and memory deficits produced by aspartame are heritable via the paternal lineage.
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Jones SK, McCarthy DM, Stanwood GD, Schatschneider C, and Bhide PG
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- Female, Male, Humans, Pregnancy, Animals, Mice, Cognition, Memory Disorders chemically induced, Memory Disorders genetics, Spatial Learning, Sweetening Agents adverse effects, Aspartame adverse effects, Drug-Related Side Effects and Adverse Reactions
- Abstract
Environmental exposures produce heritable traits that can linger in the population for one or two generations. Millions of individuals consume substances such as artificial sweeteners daily that are declared safe by regulatory agencies without evaluation of their potential heritable effects. We show that consumption of aspartame, an FDA-approved artificial sweetener, daily for up to 16-weeks at doses equivalent to only 7-15% of the FDA recommended maximum daily intake value (equivalent to 2-4 small, 8 oz diet soda drinks per day) produces significant spatial learning and memory deficits in mice. Moreover, the cognitive deficits are transmitted to male and female descendants along the paternal lineage suggesting that aspartame's adverse cognitive effects are heritable, and that they are more pervasive than current estimates, which consider effects in the directly exposed individuals only. Traditionally, deleterious environmental exposures of pregnant and nursing women are viewed as risk factors for the health of future generations. Environmental exposures of men are not considered to pose similar risks. Our findings suggest that environmental exposures of men can produce adverse impact on cognitive function in future generations and demonstrate the need for considering heritable effects via the paternal lineage as part of the regulatory evaluations of artificial sweeteners., (© 2023. Springer Nature Limited.)
- Published
- 2023
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123. Reply to Charles V. Vorhees: Aspartame and anxiety-like behavior.
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Jones SK, McCarthy DM, Vied C, Stanwood GD, Schatschneider C, and Bhide PG
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- 2023
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124. Hippocampal neuroinflammation following combined exposure to cyclophosphamide and naproxen in ovariectomized mice.
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Pavlock S, McCarthy DM, Kesarwani A, Jean-Pierre P, and Bhide PG
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- Mice, Female, Animals, Mice, Inbred C57BL, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Hippocampus, Naproxen pharmacology, Naproxen therapeutic use, Neuroinflammatory Diseases
- Abstract
Aim: Cancer patients undergoing chemotherapy report cognitive changes collectively termed "chemo brain." Neuroinflammation is among the factors believed to contribute to "chemo brain" suggesting a potential beneficial role for anti-inflammatory drugs in cancer patients undergoing chemotherapy. We investigated whether the non-steroidal anti-inflammatory drug naproxen influenced hippocampal inflammation in non-tumor bearing female mice receiving the chemotherapy drug cyclophosphamide (CP). Materials and methods: Intact and ovariectomized C57BL/6 mice were used to examine potential role of ovarian hormones on neuroinflammation. The mice were placed on naproxen (375 ppm) or control diet, and a week later CP (100 mg/kg; i.p.) was administered every 3 days for 2 weeks. We analyzed hippocampal inflammatory biomarkers, anxiety-like behavior, spatial working memory, exploratory behavior, spontaneous locomotor activity and depression-like behavior. Results: CP produced significant effects on anti-inflammatory but not pro-inflammatory biomarkers. However, CP and naproxen in combination produced significant effects on both pro- and anti- inflammatory biomarkers. Naproxen and ovariectomy individually produced significant effects on pro- and anti-inflammatory biomarkers as well. Working memory and depression-like behavior were not significantly influenced by CP, naproxen or ovariectomy individually although CP and ovariectomy produced significant interaction effects on depression-like behavior. Exploratory behavior and locomotor activity showed significant effects of CP, and interaction between CP and naproxen was significant for locomotor activity. Conclusions: Ovariectomy, naproxen and a combination of CP and naproxen upregulate hippocampal pro- and anti- inflammatory biomarkers. None of the factors individually produce significant behavioral changes that could be consistent with chemo brain, although CP and ovariectomy in combination produced significant effects on depression-like behavior, a co-morbidity of chemo brain.
- Published
- 2023
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125. Transgenerational transmission of aspartame-induced anxiety and changes in glutamate-GABA signaling and gene expression in the amygdala.
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Jones SK, McCarthy DM, Vied C, Stanwood GD, Schatschneider C, and Bhide PG
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- Humans, Female, Male, Animals, Mice, Mice, Inbred C57BL, Aspartame, Receptors, GABA-A, Anxiety chemically induced, Anxiety genetics, Amygdala, Diazepam, RNA, Messenger, Gene Expression, gamma-Aminobutyric Acid, Glutamic Acid, Drinking Water
- Abstract
We report the effects of aspartame on anxiety-like behavior, neurotransmitter signaling and gene expression in the amygdala, a brain region associated with the regulation of anxiety and fear responses. C57BL/6 mice consumed drinking water containing 0.015% or 0.03% aspartame, a dose equivalent of 8 to 15% of the FDA recommended maximum human daily intake, or plain drinking water. Robust anxiety-like behavior (evaluated using open field test and elevated zero maze) was observed in male and female mice consuming the aspartame-containing water. Diazepam, an allosteric modulator of the GABA-A receptor, alleviated the anxiety-like behavior. RNA sequencing of the amygdala followed by KEGG biological pathway analysis of differentially expressed genes showed glutamatergic and GABAergic synapse pathways as significantly enriched. Quantitative PCR showed upregulation of mRNA for the glutamate NMDA receptor subunit 2D ( Grin2d ) and metabotropic receptor 4 ( Grm4 ) and downregulation of the GABA-A receptor associated protein ( Gabarap ) mRNA. Thus, taken together, our diazepam and gene expression data show that aspartame consumption shifted the excitation-inhibition equilibrium in the amygdala toward excitation. Even more strikingly, the anxiety-like behavior, its response to diazepam, and changes in amygdala gene expression were transmitted to male and female offspring in two generations descending from the aspartame-exposed males. Extrapolation of the findings to humans suggests that aspartame consumption at doses below the FDA recommended maximum daily intake may produce neurobehavioral changes in aspartame-consuming individuals and their descendants. Thus, human population at risk of aspartame's potential mental health effects may be larger than current expectations, which only include aspartame-consuming individuals.
- Published
- 2022
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126. The adenosine A(2A) receptor agonist CGS 21680 alleviates auditory sensorimotor gating deficits and increases in accumbal CREB in rats neonatally treated with quinpirole.
- Author
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Brown RW, Bhide PG, Gill WD, and Peeters LD
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- Adenosine pharmacology, Animals, Animals, Newborn, Disease Models, Animal, Dopamine Agonists pharmacology, Dose-Response Relationship, Drug, Female, Male, Motor Activity drug effects, Nucleus Accumbens metabolism, Quinpirole pharmacology, Rats, Rats, Sprague-Dawley, Receptors, Dopamine D2 metabolism, Schizophrenia drug therapy, Schizophrenia physiopathology, Adenosine analogs & derivatives, Adenosine A2 Receptor Agonists pharmacology, Cyclic AMP Response Element-Binding Protein metabolism, Nucleus Accumbens drug effects, Phenethylamines pharmacology, Prepulse Inhibition drug effects, Receptor, Adenosine A2A metabolism, Sensory Gating drug effects
- Abstract
Rationale and Objective: The adenosine A(2A) receptor forms a mutually inhibitory heteromer with the dopamine D
2 receptor, and A(2A) agonists decrease D2 signaling. This study analyzed whether an adenosine A(2A) agonist would alleviate deficits in sensorimotor gating and increases in cyclic-AMP response element binding protein (CREB) in the nucleus accumbens (NAc) in the neonatal quinpirole model of schizophrenia (SZ)., Methods: Male and female Sprague-Dawley rats were neonatally treated with saline (NS) or quinpirole HCl (NQ; 1 mg/kg) from postnatal days (P) 1-21. Animals were raised to P44 and behaviorally tested on auditory sensorimotor gating as measured through prepulse inhibition (PPI) from P44 to P48. Approximately 15 min before each session, animals were given an ip administration of saline or the adenosine A(2A) agonist CGS 21680 (0.03 or 0.09 mg/kg). One day after PPI was complete on P49, animals were administered a locomotor activity test in the open field after saline or CGS 21680 treatment, respectively. On P50, the nucleus accumbens (NAc) was evaluated for CREB protein., Results: NQ-treated rats demonstrated a deficit in PPI that was alleviated to control levels by either dose of CGS 21680. The 0.03 mg/kg dose of CGS 21680 increased startle amplitude in males. The 0.09 mg/kg dose of CGS 21680 resulted in an overall decrease in locomotor activity. NQ treatment significantly increased NAc CREB that was attenuated to control levels by either dose of CGS 21680., Conclusions: This study revealed that an adenosine A(2A) receptor agonist was effective to alleviate PPI deficits in the NQ model of SZ in both male and female rats.- Published
- 2020
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127. Transgenerational transmission of behavioral phenotypes produced by exposure of male mice to saccharin and nicotine.
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McCarthy DM, Lowe SE, Morgan TJ, Cannon EN, Biederman J, Spencer TJ, and Bhide PG
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- Animals, Body Weight drug effects, Crosses, Genetic, DNA Methylation drug effects, DNA Methylation genetics, Drinking Behavior drug effects, Female, Male, Methylphenidate pharmacology, Mice, Inbred C57BL, Motor Activity drug effects, Phenotype, Spermatozoa drug effects, Spermatozoa metabolism, Behavior, Animal drug effects, Nicotine pharmacology, Saccharin pharmacology
- Abstract
The use of non-nutritive sweeteners such as saccharin is widely prevalent. Although saccharin is considered safe for human consumption, it produces behavioral changes in experimental animals. We report that saccharin's behavioral effects are much more pervasive than currently recognized. In a mouse model, saccharin exposure produced motor impulsivity not only in the saccharin-exposed males but also in their offspring. In addition, the offspring showed locomotor hyperactivity and working memory deficit not observed in fathers. Spermatazoal DNA was hypermethylated in the saccharin-exposed fathers, especially at dopamine receptor promoter regions, suggesting that epigenetic modification of germ cell DNA may mediate transgenerational transmission of behavioral phenotypes. Dopamine's role in hyperactivity was further highlighted by the finding that the stimulant drug methylphenidate mitigated the hyperactivity. Nicotine is another substance that is widely used. Its use via smokeless tobacco products, some of which contain saccharin, is on the rise contributing to concerns about adverse outcomes of co-exposure to saccharin and nicotine. We found that co-exposure of male mice to saccharin and nicotine produced significant behavioral impairment in their offspring. Thus, our data point to potential adverse neurobehavioral consequences of exposure to saccharin alone or saccharin and nicotine for the exposed individuals and their descendants.
- Published
- 2020
- Full Text
- View/download PDF
128. Effects of Developmental Nicotine Exposure on Frontal Cortical GABA-to-Non-GABA Neuron Ratio and Novelty-Seeking Behavior.
- Author
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Martin MM, McCarthy DM, Schatschneider C, Trupiano MX, Jones SK, Kalluri A, and Bhide PG
- Subjects
- Animals, Behavior, Animal drug effects, Disease Models, Animal, Female, Frontal Lobe drug effects, Memory Disorders complications, Memory, Short-Term drug effects, Mice, Prefrontal Cortex drug effects, Pregnancy, Prenatal Exposure Delayed Effects, Exploratory Behavior drug effects, GABAergic Neurons drug effects, Nicotine pharmacology, Nicotinic Agonists pharmacology
- Abstract
Cigarette smoking during pregnancy is a major public health concern, resulting in detrimental health effects in the mother and her offspring. The adverse behavioral consequences for children include increased risk for attention deficit hyperactivity disorder, working memory deficits, epilepsy, novelty-seeking, and risk-taking behaviors. Some of these behavioral conditions are consistent with an imbalance in frontal cortical excitatory (glutamate) and inhibitory (GABA) neurotransmitter signaling. We used a GAD67-GFP knock-in mouse model to examine if developmental nicotine exposure alters frontal cortical GABA neuron numbers, GABA-to-non-GABA neuron ratio and behavioral phenotypes. Female mice were exposed to nicotine (100 or 200 μg/mL) in drinking water beginning 3 weeks prior to breeding and until 3 weeks postpartum. Male and female offspring were examined beginning at 60 days of age. The nicotine exposure produced dose-dependent decreases in GABA-to-non-GABA neuron ratios in the prefrontal and medial prefrontal cortices without perturbing the intrinsic differences in cortical thickness and laminar distribution of GABA or non-GABA neurons between these regions. A significant increase in exploratory behavior and a shift toward "approach" in the approach-avoidance paradigm were also observed. Thus, developmental nicotine exposure shifts the cortical excitation-inhibition balance toward excitation and produces behavioral changes consistent with novelty-seeking behavior., (© The Author(s) 2019. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.)
- Published
- 2020
- Full Text
- View/download PDF
129. Effects of prenatal exposure to cocaine on brain structure and function.
- Author
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McCarthy DM, Kabir ZD, Bhide PG, and Kosofsky BE
- Subjects
- Animals, Disease Models, Animal, Female, Mice, Pregnancy, Prenatal Exposure Delayed Effects, Brain drug effects, Cocaine toxicity, Dopamine Uptake Inhibitors toxicity, Neurogenesis drug effects
- Abstract
Drug abuse during pregnancy affects the mother and has adverse effects on the unborn child. This chapter highlights our recent findings at the neuroanatomical, molecular, and behavioral levels in a prenatal cocaine exposure mouse model. In the embryonic brains of prenatally cocaine-exposed mice, we observed a delay in the tangential migration of GABA neurons to the cerebral cortex as a result of a significant but transient decrease in the expression of the neurotrophin brain-derived neurotrophic factor (BDNF). These developmental changes lead to lasting deficits in the numerical density of GABA neurons in the mature medial prefrontal cortex (mPFC). In adult prenatally cocaine-exposed mice, we observed a behavioral deficit in the recall of an extinguished cue-conditioned fear, which was rescued by administration of exogenous recombinant BDNF protein directly into the infralimbic cortex of the mPFC, which may result from altered activity-driven transcriptional regulation of BDNF., (© 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
130. Prenatal nicotine exposure mouse model showing hyperactivity, reduced cingulate cortex volume, reduced dopamine turnover, and responsiveness to oral methylphenidate treatment.
- Author
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Zhu J, Zhang X, Xu Y, Spencer TJ, Biederman J, and Bhide PG
- Subjects
- Animals, Attention Deficit Disorder with Hyperactivity physiopathology, Disease Models, Animal, Female, Gyrus Cinguli pathology, Gyrus Cinguli physiopathology, Male, Mice, Mice, Inbred C57BL, Models, Neurological, Pregnancy, Prenatal Exposure Delayed Effects pathology, Prenatal Exposure Delayed Effects physiopathology, Smoking adverse effects, Attention Deficit Disorder with Hyperactivity chemically induced, Attention Deficit Disorder with Hyperactivity drug therapy, Dopamine metabolism, Gyrus Cinguli drug effects, Methylphenidate pharmacology, Nicotine toxicity, Prenatal Exposure Delayed Effects chemically induced
- Abstract
Cigarette smoking, nicotine replacement therapy, and smokeless tobacco use during pregnancy are associated with cognitive disabilities later in life in children exposed prenatally to nicotine. The disabilities include attention deficit hyperactivity disorder (ADHD) and conduct disorder. However, the structural and neurochemical bases of these cognitive deficits remain unclear. Using a mouse model we show that prenatal nicotine exposure produces hyperactivity, selective decreases in cingulate cortical volume, and radial thickness, as well as decreased dopamine turnover in the frontal cortex. The hyperactivity occurs in both male and female offspring and peaks during the "active" or dark phase of the light/dark cycle. These features of the mouse model closely parallel the human ADHD phenotype, whether or not the ADHD is associated with prenatal nicotine exposure. A single oral, but not intraperitoneal, administration of a therapeutic equivalent dose (0.75 mg/kg) of methylphenidate decreases the hyperactivity and increases the dopamine turnover in the frontal cortex of the prenatally nicotine exposed mice, once again paralleling the therapeutic effects of this compound in ADHD subjects. Collectively, our data suggest that the prenatal nicotine exposure mouse model has striking parallels to the ADHD phenotype not only in behavioral, neuroanatomical, and neurochemical features, but also with respect to responsiveness of the behavioral phenotype to methylphenidate treatment. The behavioral, neurochemical, and anatomical biomarkers in the mouse model could be valuable for evaluating new therapies for ADHD and mechanistic investigations into its etiology.
- Published
- 2012
- Full Text
- View/download PDF
131. Neuro-developmental consequences of prenatal drug exposure. Preface.
- Author
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Bhide PG and Kosofsky BE
- Subjects
- Adult, Female, Humans, Pregnancy, Prenatal Exposure Delayed Effects, Substance-Related Disorders complications
- Published
- 2009
- Full Text
- View/download PDF
132. Involvement of matrix metalloproteinase in neuroblast cell migration from the subventricular zone after stroke.
- Author
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Lee SR, Kim HY, Rogowska J, Zhao BQ, Bhide P, Parent JM, and Lo EH
- Subjects
- Animals, Cell Movement, Cells, Cultured, Corpus Striatum metabolism, Corpus Striatum pathology, Male, Mice, Nerve Regeneration physiology, Neuronal Plasticity, Recovery of Function physiology, Cerebral Ventricles enzymology, Cerebral Ventricles pathology, Matrix Metalloproteinases metabolism, Neurons enzymology, Neurons pathology, Stroke enzymology, Stroke pathology
- Abstract
After brain injury, neuroblast cells from the subventricular zone (SVZ) expand and migrate toward damaged tissue. The mechanisms that mediate these neurogenic and migratory responses remain to be fully dissected. Here, we show that bromodeoxyuridine-labeled and doublecortin-positive cells from the SVZ colocalize with the extracellular protease matrix metalloproteinase-9 (MMP-9) during the 2 week recovery period after transient focal cerebral ischemia in mice. Treatment with the broad spectrum MMP inhibitor GM6001 significantly decreases the migration of doublecortin-positive cells that extend from the SVZ into the striatum. These data suggest that MMPs are involved in endogenous mechanisms of neurogenic migration as the brain seeks to heal itself after injury.
- Published
- 2006
- Full Text
- View/download PDF
133. Analysis of cell generation in the telencephalic neuroepithelium.
- Author
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Takahashi T, Caviness VS Jr, and Bhide PG
- Subjects
- Animals, Bromodeoxyuridine, Cell Lineage physiology, Cell Movement physiology, Epithelium metabolism, Immunohistochemistry, Mice, Neuroglia cytology, Neurons cytology, Stem Cells cytology, Telencephalon cytology, Telencephalon metabolism, Thymidine, Tritium, Autoradiography methods, Epithelium embryology, Neuroglia metabolism, Neurons metabolism, S Phase physiology, Stem Cells metabolism, Telencephalon embryology
- Published
- 2002
- Full Text
- View/download PDF
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