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393 results on '"Bertolotti, Luigi"'

101. Genetic characterization of bovine respiratory syncytial virus strains isolated in Italy: evidence for the circulation of new divergent clades.

Author

Bertolotti, Luigi, Giammarioli, Monica, and Rosati, Sergio

Subjects
BOVINE respiratory syncytial virus, HEALTH of cattle
Abstract

Bovine respiratory syncytial virus (BRSV) is circulating in cattle in Europe. Although vaccination helps control the disease, its prevalence within and among herds remains high. Previous genetic characterization studies revealed a strict geographic correlation between viral variants; on the other hand, they showed the emergence of new variants in northern Europe. Few studies have described BRSV distribution, and little is known about the genetic features of BRSV strains circulating in Italy. We studied sample-positive tests for BRSV, and sequenced the coding regions of the G and N proteins to determine the presence of divergent variants. Two different sets of sequences were found, including in samples from animals from vaccinated herds. The 2 groups of sequences correspond to 2 time periods and suggest an active role of herd immunity in preventing the spread of infection. Our findings that different strains of BRSV are circulating in Italy and that the virus is evolving rapidly highlight the importance of updating vaccination strategies. [ABSTRACT FROM AUTHOR]

Published
2018
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105. Contemporary use of acepromazine in the anaesthetic management of male horses and ponies: a retrospective study and opinion poll

Author

Driessen, B., Zarucco, Laura, Kalir, B., and Bertolotti, Luigi

Subjects
Male, Sex Characteristics, Penile Diseases, Data Collection, Surveys and Questionnaires, Animals, Hypnotics and Sedatives, Anesthesia, Female, Horse Diseases, Horses, Hypotension, Acepromazine, Retrospective Studies
Abstract

Current use of acepromazine in the anaesthetic management of male horses and ponies and associated risks are largely unknown.To explore anaesthetic acepromazine use and related adverse effects in the male horse.Of 8533 anaesthetised horses and ponies medical records of male animals treated perianaesthetically with acepromazine were reviewed. Demographic data, time and dose of acepromazine administration, co-administered drugs, quality of induction and recovery from anaesthesia, arterial blood pressures, and occurrence of penile dysfunction were recorded. Practising ACVA and ECVAA diplomates were polled on the use of acepromazine and its effects on blood pressure and penile dysfunction in the equine.Of all animals, 12% females and 11% males (n=575 including 42% stallions) received perianaesthetic acepromazine, predominantly for premedication. Anaesthetic induction was smooth in 566 animals. Lowest mean arterial pressures averaged 65±9 mmHg. Recovery was good or very good in 70% of all animals and 74% stood after 1-2 attempts. In 14 horses (2.4%; 7 stallions, 7 geldings), penile prolapse occurred for 0.5-4 h and in one stallion (0.2%) for12 but18 h post recovery. Most surveyed anaesthesiologists use acepromazine in stallions (occasionally 63%; frequently 17%) but more frequently in geldings (occasionally 34%; frequently 59%) and mares (occasionally 38%; frequently 59%), primarily for premedication with other sedatives and analgesics. Persistent intraoperative hypotension was not frequently reported. Only 5% of surveyed anaesthesiologists recall penile prolapse post acepromazine administration lasting for12 h and only one recalls 3 cases of irreversible penile prolapse in 20 years of anaesthesia practice.The extremely low risk of permanent penile dysfunction (≤1 in 10,000 cases) does not justify more restricted use of acepromazine in the intact male vs. geldings and mares.

Published
2010

116. Genetic traceability of two local chicken populations, Bianca di Saluzzo and Bionda Piemontese, versus some current commercial lines

Author

Sartore, Stefano, primary, Soglia, Dominga, additional, Maione, Sandra, additional, Sacchi, Paola, additional, De Marco, Michele, additional, Schiavone, Achille, additional, Sponza, Simone, additional, Dalmasso, Alessandra, additional, Bottero, Maria Teresa, additional, Pattono, Daniele, additional, Zoccarato, Ivo, additional, Gasco, Laura, additional, Brugiapaglia, Alberto, additional, Tarantola, Martina, additional, Giacobini, Mario, additional, Bertolotti, Luigi, additional, and Rasero, Roberto, additional

Published
2014
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139. Borrelia lusitaniae and spotted fever group rickettsiae in Ixodes ricinus (Acari: Ixodidae) in Tuscany, central Italy

Author

Bertolotti, Luigi, Tomassone, Laura, Tramuta, Clara, Grego, Elena, Amore, Giuseppina, Ambrogi, C, Nebbia, Patrizia, and Mannelli, Alessandro

Subjects
Nymph, Lyme Disease, Ixodes, spotted fever group rickettsiae, Borrelia lusitaniae, Ixodes ricinus, Rhipicephalus, Italy, Borrelia, Glutamate Synthase, Rickettsia Infections, Polymerase Chain Reaction, RNA, Ribosomal, 16S, DNA, Ribosomal Spacer, Prevalence, Animals, Humans, Arachnid Vectors, Rickettsia, Phylogeny, Bacterial Outer Membrane Proteins
Abstract

Prevalence of infection by Borrelia burgdorferi s.l. and spotted fever group (SFG) rickettsiae was estimated in host-seeking ticks in an area in Tuscany, central Italy, where Lyme borreliosis was reported in a forestry worker. B. burgdorferi s.l. was identified by polymerase chain reaction in 16.7% (95% CI = 10.3, 24.8) of Ixodes ricinus (L.) nymphs and 39.6% (95% CI = 26.5, 54.0) of adults. Borrelia lusitaniae accounted for 82.9% of positive samples, followed by Borrelia garinii (9.8%), Borrelia afzelii (2.4%), and Borrelia burgdorferi s.s. (2.4%). One Rhipicephalus spp. adult was infected with B. garinii (prevalence = 8.3%; 95% CI = 0.21, 38.5). Prevalence of infection by SFG rickettsiae was 38.5% (95% CI = 26.7, 51.4) in I. ricinus nymphs, 34.6% (95% CI = 22.0, 49.1) in I. ricinus adults, and 50% (95% CI = 21.1, 78.9) in Rhipicephalus spp. adults. Phylogenetic analysis showed the similarity of B. lusitaniae strains that were identified in this study and of a strain that was previously isolated from a human patient in Portugal. Results of this study confirm the dominance of B. lusitaniae in areas in the Mediterranean basin and the infection by SFG rickettsiae in I. ricinus.

Published
2006

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