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Human leptospirosis is a zoonotic and potentially fatal disease that has increasingly been reported in both developing and developed countries, including France. However, our understanding of the basic aspects of the epidemiology of this disease, including the source of Leptospira serogroup Australis infections in humans and domestic animals, remains incomplete. We investigated the genetic diversity of Leptospira in 28 species of wildlife other than rats using variable number tandem repeat (VNTR) and multispacer sequence typing (MST). The DNA of pathogenic Leptospira was detected in the kidney tissues of 201 individuals out of 3,738 tested individuals. A wide diversity, including 50 VNTR profiles and 8 MST profiles, was observed. Hedgehogs and mustelid species had the highest risk of being infected (logistic regression, OR = 66.8, CI95% = 30.9-144 and OR = 16.7, CI95% = 8.7-31.8, respectively). Almost all genetic profiles obtained from the hedgehogs were related to Leptospira interrogans Australis, suggesting the latter as a host-adapted bacterium, whereas mustelid species were infected by various genotypes, suggesting their interaction with Leptospira was different. By providing an inventory of the circulating strains of Leptospira and by pointing to hedgehogs as a potential reservoir of L. interrogans Australis, our study advances current knowledge on Leptospira animal carriers, and this information could serve to enhance epidemiological investigations in the future.

A good knowledge of rainfall is essential for hydrological operational purposes such as flood forecasting. The objective of this paper was to analyze, on a relatively large sample of flood events, how rainfall-runoff modeling using an event-based model can be sensitive to the use of spatial rainfall compared to mean areal rainfall over the watershed. This comparison was based not only on the model's efficiency in reproducing the flood events but also through the estimation of the initial conditions by the model, using different rainfall inputs. The initial conditions of soil moisture are indeed a key factor for flood modeling in the Mediterranean region. In order to provide a soil moisture index that could be related to the initial condition of the model, the soil moisture output of the Safran-Isba-Modcou (SIM) model developed by Météo-France was used. This study was done in the Gardon catchment (545 km2) in South France, using uniform or spatial rainfall data derived from rain gauge and radar for 16 flood events. The event-based model considered combines the SCS runoff production model and the Lag and Route routing model. Results show that spatial rainfall increases the efficiency of the model. The advantage of using spatial rainfall is marked for some of the largest flood events. In addition, the relationship between the model's initial condition and the external predictor of soil moisture provided by the SIM model is better when using spatial rainfall, in particular when using spatial radar data with R2 values increasing from 0.61 to 0.72.

Urban leptospirosis has increasingly been reported in both developing and developed countries. The control of the disease is limited because our understanding of basic aspects of the epidemiology, including the transmission routes of leptospires among rat populations, remains incomplete. Through the ability to distinguish among Leptospira strains in rats, multispacer sequence typing (MST) could provide a modern understanding of Leptospira epidemiology; however, to our knowledge, the distribution of Leptospira strains among urban rat colonies has not been investigated using MST.The objective of this study was to identify the Leptospira strains present in rats (Rattus norvegicus) in Lyon (France) using MST and to characterize their spatial distribution. Kidneys and urine were collected from rats trapped live in seven locations in the city and in one suburban location. Each location was considered to represent a rat colony. Bacterial cultures and quantitative polymerase chain reaction (qPCR) assays were performed, and the L. interrogans DNA identified was then genotyped using MST. The distributions of Leptospira strains were spatially described.Among 84 wild rats, MST profiles were obtained in 35 of 37 rats that had a positive result for L. interrogans by bacterial culture and/or qPCR analyses. All of the MST profiles were related to reference strains previously isolated from human patients that belong to the serogroup Icterohaemorrhagiae and the serovars [strain(s)] Copenhageni [Wijinberg or M20] (n = 26), Icterohaemorrhagiae [CHU Réunion] (n = 7), Icterohaemorrhagiae [R1] (n = 1) and Copenhageni [Shibaura 9] (n = 1). Each colony was infected with leptospires having the same MST profile.This study demonstrated that MST could be used for the purpose of field studies, either on culture isolates or on DNA extracted from kidneys and urine, to distinguish among L. interrogans isolates in rats. MST could thus be used to monitor their distributions in urban rats from the same city, thereby providing new knowledge that could be applied to explore the circulation of L. interrogans infection in rat colonies. Because the strains are related to those previously found in humans, this application of MST could aid in the source tracking of human leptospirosis, and the findings would be relevant for public health purposes according to the One Health principle.

OBJECTIVE: Cholesteatoma (CHO) developing secondary to chronic otitis media (COM) can spread rapidly and cause important health problems such as hearing loss. Therefore, the presence of CHO should be diagnosed promptly with high accuracy and then treated surgically. The aim of this study was to investigate the effectiveness of artificial intelligence applications (AIA) in documenting the presence of CHO based on computed tomography (CT) images. PATIENTS AND METHODS: The study was performed on CT images of 100 CHO, 100 non-cholesteatoma (N-CHO) COM, and 100 control patients. Two AIA models including Res- Net50 and MobileNetV2 were used for the classification of the images. RESULTS: Overall accuracy rate was 93.33% for the ResNet50 model and 86.67% for the MobilNetV2 model. Moreover, the diagnostic accuracy rates of these two models were 100% and 95% in the CHO group, 90% and 85% in the N-CHO group, and 90% and 80% in the control group, respectively. CONCLUSIONS: These results indicate that the use of AIA in the diagnosis of CHO will improve the diagnostic accuracy rates and will also help physicians in terms of reducing their workload and facilitating the selection of the correct treatment strategy. [ABSTRACT FROM AUTHOR]

During wildland fires, wildland-urban interfaces may cause significant problems for emergency managers throughout the world. Several works focused on safety zones aiming to prevent houses to burn, but no work focused on the LPG tanks which can be located in the surroundings of houses. A finite elements modelling was developed to calculate safety distance preventing from BLEVE. The safety distances required by law in several countries (50 m; 30 m; 25 m) are checked. It appears that the required safety distances are correct to prevent the tank from BLEVE.

The Mediterranean area is prone to intense rainfall events triggering flash floods, characterized by very short response times that sometimes lead to dramatic consequences in terms of casualties and damages. These events can affect large territories, but their impact may be very local in catchments that are generally ungauged. These events remain difficult to predict and the processes leading to their generation still need to be clarified. The HyMeX initiative (Hydrological Cycle in the Mediterranean Experiment, 2010-2020) aims at increasing our understanding of the water cycle in the Mediterranean basin, in particular in terms of extreme events. In order to better understand processes leading to flash floods, a four-year experiment (2012-2015) was conducted in the Cévennes region (South-East) France as part of the FloodScale project. Both continuous and opportunistic measurements during floods were conducted in two large catchments (Ardèche and Gard rivers) with nested instrumentation from the hillslopes to catchments of about 1, 10, 100 to 1000 km2 covering contrasted geology and land use. Continuous measurements include distributed rainfall, stream water level, discharge, water temperature and conductivity and soil moisture measurements. Opportunistic measurements include surface soil moisture and geochemistry sampling during events and gauging of floods using non-contact methods: portable radars to measure surface water velocity or image sequence analysis using LS-PIV (Large Scale Particle Image Velocimetry). During the period 2012-2014, and in particular during autumn 2014, several intense events affected the catchments and provided very rich data sets. Data collection was complemented with modelling activity aiming at simulating observed processes. The modelling strategy was setup through a wide range of scales, in order to test hypotheses about physical processes at the smallest scales, and aggregated functioning hypothesis at the largest scales. During the project, a focus was also put on the improvement of rainfall fields characterization both in terms of spatial and temporal variability and in terms of uncertainty quantification. Rainfall reanalyses combining radar and rain gauges were developed. Rainfall simulation using a stochastic generator was also performed. Another effort was dedicated to the improvement of discharge estimation during floods and the quantification of streamflow uncertainties using Bayesian techniques. The paper summarizes the main results gained from the observations and the subsequent modelling activity in terms of flash flood process understanding at the various scales. It concludes on how the new acquired knowledge can be used for prevention and management of flash floods.

In our study, the effect of Frankel 3 appliance on nasal resistance was evaluated in Angle Class 3 cases. For the study, 7 females and 13 males, total 20 individuals were treated as treatment group as well as 7 females and 7 males total 14 individuals were members of control group. The planimetric measurements were done on the lateral cephalometric radiograms taken before and after the treatment. As a result of this study, a decreased nasal resistance was found by using “Mann Whitney U” test, in the individuals which were treated with Frankel 3 appliance. And also by using “Student t” test, it was observed that the location of the tongue and the mandible had not gained any change.

In the reestablishment of the impaired function and facial esthetics of the cleft lip and palate patients, orthodontic and maxillary orthopedic treatment has a determinant contribution within a multidisciplinary team approach. This philosophy of therapy is rendered in two time periods, a) Preoperative maxillary orthopedic treatment or infant orthopedics, b) Postoperative maxillary orthopedic and orthodontic treatment. Currently the benefits of early rnaxillary orthopedic treatment are still controversial The objective pursued here is to help establish a maxillary arch form as a fundamental base prior to the closure of the lip without the formation of the scar tissue. There is substantial evidence that the main factor restraining the development of the maxilla is not the early palatoplasty but failure of the lip construction. In the postoperative period, treatment is concentrated on the expansion of the collapsed rnaxillary arch and/or anterior maxillary growth stimulation via orthopedic force application. The intention of this paper is to present the pre and post operative treatment rationale of our clinic in CLP patients.

The recent novelties in orthodontic materials and mechanics have enhanced the indication of non extraction treatment procedures as it not only provides a functional and stable occlusion but also is an easier treatment of choice in the eye of patients and parents. Furthermore, it substantially reduces treatment time in a majority of malocclusions. This article has demonstrated various non extraction instances and mechanics along with their clinical management.

OBJECTIVE: Amikacin (AMK) is a widely used antibiotic, but its ototoxic side effects limit its use. This study investigated the effects of ethyl pyruvate (EP), known for its antioxidant and anti-inflammatory effects, against AMK ototoxicity. MATERIALS AND METHODS: 32 Wistar albino rats (n: 8) were used in this study. To cause ototoxicity, AMK 600 mg/kg/day dose was applied intramuscularly for 14 days. EP was administered via ip at a dose of 50 mg/kg/day for 14 days. RESULTS: The Auditory Brainstem Responses (ABR) and Distortion Product Otoacoustic Emissions (DPOAE) tests were performed on the study's 0, 7, and 14 days. The results have shown that the hearing functions were significantly impaired with the AMK application. A significant improvement was observed in the AMK+ EP group. While total oxidant status (TOS), oxidative stress index (OSI), and malondialdehyde (MDA) levels were found to be significantly higher in the AMK group compared to the control group, total antioxidant status (TAS) level was found to be significantly lower. In the AMK+ EP group, on the other hand, deterioration in TOS, OSI, and MDA levels detected in the AMK group was not observed. No elevated pro-inflammatory cytokines, such as TNF-a, IL-1ß, and IL-6 were present in the EP+AMK group, which were detected in the AMK group. CONCLUSIONS: Hearing tests and biochemical results show that ethyl pyruvate has protective effects against amikacin ototoxicity due to its antioxidant and anti-inflammatory effects. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Parotid surgery is a frequently performed surgery in otorhinolaryngology practice with many possible complications. Due to the high ratio of facial paralysis during parotid surgery, we defined a new landmark for identifying and protecting the facial nerve as early as possible during surgery. MATERIALS AND METHODS: The study was designed as a prospective anatomical method. The important details and relationship of the tragomastoid groove to the facial nerve truncus were examined during surgery on 30 patients. In addition, the demographics of the patients, the type of surgery and the pathological results of surgeries were evaluated. RESULTS: The mean distance of the tragomastoid groove to the facial nerve truncus was 20.53±1.71 mm, the mean deepness of the tragomastoid groove was 1.91±0.26 mm, and the mean superficial part of the tragomastoid groove was 0.83±0.23 mm. The tragomastoid groove was situated either across from the facial nerve at the place where the facial nerve truncus exits the stylomastoid foramen or just inferior to the truncus in all patients. CONCLUSIONS: The tragomastoid groove was defined for the first time in the literature as a reliable landmark for identifying the facial nerve truncus easily during parotid surgery. [ABSTRACT FROM AUTHOR]

No abstract available.