101. LeEIX2 Interactors' Analysis and EIX-Mediated Responses Measurement.
- Author
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Leibman-Markus M, Schuster S, and Avni A
- Subjects
- Ethylenes analysis, Fungal Proteins metabolism, Immunoprecipitation, Solanum lycopersicum metabolism, Plant Immunity, Protein Binding, Protein Domains, Reactive Oxygen Species analysis, Signal Transduction, Nicotiana metabolism, Fungal Proteins immunology, Solanum lycopersicum immunology, Plant Proteins metabolism, Nicotiana immunology
- Abstract
Plant-pathogen interactions involve a large number of wide regulatory systems, necessary for plant defense responses against pathogen attack. The fungal protein ethylene-inducing xylanase (EIX) elicits defense responses in specific cultivars of tobacco and tomato. The response to EIX is controlled by a single locus encoding for LeEIX2, a leucine-rich-repeat receptor-like-protein (LRR-RLP). As an RLP, LeEIX2 does not possess an obvious cytoplasmic signaling moiety such as a kinase domain. To study LeEIX2 mode of action, it is essential to identify the potential interactors involved after EIX perception. Here, we describe the in vivo co-IP methodology used for protein interaction verification and ethylene and ROS (reactive oxygen species) measurements used for physiological effects assessment.
- Published
- 2017
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