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102. Genome-wide identification and in-silico expression analysis of carotenoid cleavage oxygenases gene family in Oryza sativa (rice) in response to abiotic stress

Author

Haider, Muhammad Zeshan, primary, Sami, Adnan, additional, Shafiq, Muhammad, additional, Anwar, Waheed, additional, Ali, Sajid, additional, Ali, Qurban, additional, Muhammad, Sher, additional, Manzoor, Irfan, additional, Shahid, Muhammad Adnan, additional, Ali, Daoud, additional, and Alarifi, Saud, additional

Published
2023
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105. Bacterial and Fungal Biocontrol Agents for Plant Disease Protection: Journey from Lab to Field, Current Status, Challenges, and Global Perspectives

Author

Ayaz, Muhammad, primary, Li, Cai-Hong, additional, Ali, Qurban, additional, Zhao, Wei, additional, Chi, Yuan-Kai, additional, Shafiq, Muhammad, additional, Ali, Farman, additional, Yu, Xi-Yue, additional, Yu, Qing, additional, Zhao, Jing-Tian, additional, Yu, Jing-Wen, additional, Qi, Ren-De, additional, and Huang, Wen-Kun, additional

Published
2023
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106. degQ associated with the degS/degU two‐component system regulates biofilm formation, antimicrobial metabolite production, and biocontrol activity in Bacillus velezensis DMW1

Author

Yu, Chenjie, primary, Qiao, Junqing, additional, Ali, Qurban, additional, Jiang, Qifan, additional, Song, Yan, additional, Zhu, Linli, additional, Gu, Qin, additional, Borriss, Rainer, additional, Dong, Suomeng, additional, Gao, Xuewen, additional, and Wu, Huijun, additional

Published
2023
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107. Cytotoxic Activity of Phytoconstituents Isolated from Monotheca buxifolia against Hepatocellular Carcinoma Cell Line HepG2: In Vitro and Molecular Docking Studies

Author

Hassan, Said, primary, Ahmad, Bashir, additional, Khan, Muhammad Waseem, additional, Shah, Zafar Abbass, additional, Ullah, Amin, additional, Ullah, Sana, additional, Khan, Dilfaraz, additional, Rizwan, Muhammad, additional, Ahmad, Ajaz, additional, Ali, Qurban, additional, Kaushik, Prashant, additional, and Yilmaz, Semih, additional

Published
2023
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111. LC-MS/MS-based metabolomics approach identified novel antioxidant flavonoids associated with drought tolerance in citrus species

Author

Rao, Muhammad Junaid, primary, Feng, Bihong, additional, Ahmad, Muhammad Husnain, additional, Tahir ul Qamar, Muhammad, additional, Aslam, Muhammad Zeshan, additional, Khalid, Muhammad Fasih, additional, Hussain, Sajjad, additional, Zhong, Ruimin, additional, Ali, Qurban, additional, Xu, Qiang, additional, Ma, Chongjian, additional, and Wang, Lingqiang, additional

Published
2023
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118. Probing Antibacterial and Anticancer Potential of Selenicereus undatus , Pistacia vera L. and Olea europaea L. against Uropathogens, MCF-7 and A2780 Cancer Cells.

Author

Safdar, Sahar, Shamim, Saba, Khan, Maryam, Imran, Ali, Khan, Mudassar Ali, Ali, Qurban, and Han, Shiming

Subjects
OLIVE, CANCER cells, PISTACHIO, URINARY tract infections, PLANT extracts, DRUG resistance in bacteria, PHYTOCHEMICALS, CHLOROFORM
Abstract

Urinary tract infection is an infectious disease that requires immediate treatment. It can occur in any age group and involves both genders equally. The present study was to check the resistance of some antibiotics and to assess the antibacterial potential of three extracts of three plants against notorious bacteria involved in urinary tract infections. Along with assessing the antibacterial activity of plant extracts, we checked for the anticancer potential of these extracts against the cancer cell lines MCF-7 and A2780. Cancer is the leading cause of mortality in developed countries. Determinations of total flavonoid content, total phenolic content, total alkaloid content, total tannin content, total carotenoid content, and total steroid content were performed. The disk diffusion method was used to analyze the antibacterial activity of plant extracts. Ethanolic extract of Selenicereus undatus showed sensitivity (25–28 mm) against bacteria, whereas chloroform and hexane extracts showed resistance against all bacteria except Staphylococcus (25 mm). Ethanolic extract of Pistacia vera L. showed sensitivity (22–25 mm) against bacteria, whereas chloroform and hexane extracts showed resistance. Ethanolic extract of Olea europaea L. showed sensitivity (8–16 mm) against all bacteria except Staphylococcus, whereas chloroform and hexane extracts showed resistance. Positive controls showed variable zones of inhibition (2–60 mm), and negative control showed 0–1 mm. The antibiotic resistance was much more prominent in the case of hexane and chloroform extracts of all plants, whereas ethanolic extract showed a sensitivity of bacteria against extracts. Both cell lines, MCF-7 and A2780, displayed decreased live cells when treated with plant extracts. [ABSTRACT FROM AUTHOR]

Published
2023
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119. Empowering Educational Institutions: The Synergy of Green HR Practices and Employee Wisdom for Elevated Workplace Wellbeing.

Author

Akhtar, Massaud, Anwar, Bilal, and Ali, Qurban

Subjects
EMPLOYEE well-being, SELF-efficacy, SUSTAINABILITY, WORK environment, WISDOM
Abstract

In today's global push towards sustainability, the dynamics between Green Human Resource Practices (GHRP), Employee Wisdom (EW), and Workplace Well-being (WWB) have garnered significant attention. This study was set against the backdrop of educational institutions in Punjab, Pakistan, with the intent to elucidate these interrelationships. Using a quantitative research approach, we gathered data from employees through comprehensive surveys and subsequently analyzed it using the PLS-SEM technique. The findings revealed a robust positive relationship between GHRP and WWB. Furthermore, EW emerged as a pivotal mediator, bridging the gap between GHRP and WWB. Notably, the combined impact of GHRP and EW on WWB was found to be more profound than when they were considered separately. These results underscore the imperative of integrating sustainable HR practices in educational settings and the value of cultivating employee wisdom. Beyond promoting eco-friendly initiatives, the research emphasizes the resultant enhancement in the work environment and the overall well-being of staff members. This study augments the existing literature, offering a unique perspective on the intertwined roles of GHRP, EW, and WWB in the educational milieu of Punjab. [ABSTRACT FROM AUTHOR]

Published
2023
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120. Phycoremediation of industrial wastewater using Vaucheria debaryana and Cladophora glomerata

Author

Aman, Komal, Habib, Mudassir, Ullah, Amin, YILMAZ, SEMİH, Ayaz, Tehreem, Aziz, Aamir, Ali, Qurban, Khan, Sara, Farid, Arshad, and Yasmin, Humaira

Abstract

Rapid urbanization and industrialization are regarded as the leading causes of environmental pollution, mainly aquatic pollution. This study was carried out to investigate the use of algal species Cladophora glomerata (CG) and Vaucheria debaryana (VD) as a cost-effective and environmentally friendly phycoremediators for composite industrial effluent. After the pot experimentation using algal species, a considerable decrease in electrical conductivity (EC: 49.10–81.46%), dissolved oxygen (DO: 3.76–8.60%), biological oxygen demand (BOD: 7.81–39.28%), chemical oxygen demand (COD: 7.81–39.28%), total suspended solids (TSS: 38.09–62.21%), and total dissolved solids (TDS: 38.09–62.21%) was observed. Before and after experimentation, the heavy metals were also quantified using atomic absorption spectrophotometry (AAS), and considerable reduction was observed in Cd (41.02–48.75%) and Pb (48.72–57.03%) concentrations. The Cd concentration determined in CTCG (control treatment for Cladophora glomerata containing tap water), CG (treatment pot for Cladophora glomerata containing industrial effluents), CTVD (control pot for Vaucheria debaryana containing tap water), and VD (treatment pot for Vaucheria debaryana containing industrial effluents) biomass was 0.06, 0.499, 0.035, and 0.476 mg/kg, respectively. The Pb uptake determined in CTCG, CG, CTVD, and VD was 0.32, 1.12, 0.31, and 0.49 mg/kg, respectively, using wet digestion method and ASS. The data revealed that C. glomerata has the highest bioconcentration factor for Cd (98.42%), followed by Pb (92.57%) in treatment pots containing industrial effluents (CG and VD). Furthermore, C. glomerata showed the highest bioconcentration factor for Pb (86.49%) as compared to Cd (75%) in tap water (CTCG and CTVD). The t test analysis revealed that heavy metal concentrations significantly (p ≤ 0.05) reduced through the phycoremediation process. The analysis found that C. glomerata removed 48.75% of Cd and 57.027% of Pb from industrial effluents. Phytotoxicity assay was also performed by cultivating Triticum sp. in order to analyze the toxicity of the untreated (control) and treated water samples. Phytotoxicity result shows that the effluent treated with both Cladophora glomerata and Vaucheria debaryana gives better wheat (Triticum sp.) plant % germination, plant height (cm), and root height (cm). The highest plant % germination was showed by treated CTCG (90%), followed by CTVD (80%) and CG (70%) and VD (70%). The study concluded that phycoremediation using C. glomerata and V. debaryana is one of the environment-friendly approaches. The proposed algal-based strategy is economically viable and environmentally sustainable that can be utilized for the remediation of industrial effluents.

Published
2023

124. Genome-Wide Identification, and In-Silico Expression Analysis of YABBY Gene Family in Response to Biotic and Abiotic Stresses in Potato (Solanum tuberosum)

Author

Mazhar, Hafiz Sabah-Ud-Din, primary, Shafiq, Muhammad, additional, Ali, Haider, additional, Ashfaq, Muhammad, additional, Anwar, Alia, additional, Tabassum, Javaria, additional, Ali, Qurban, additional, Jilani, Ghulam, additional, Awais, Muhammad, additional, Sahu, Ravi, additional, and Javed, Muhammad Arshad, additional

Published
2023
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125. In Vivo and in Vitro Evaluation of Achyranthes Bidentata Leaf Extract as a Potential Antidiabetic Agent

Author

Ali, Muhsin, Khan, Muhammad Ajmal, Yasmin, Humaira, Khan, Bakhtawar, Amin, Aftab, Ahmad, Ajaz, Farid, Awais, Ullah, Amin, Butt, Sadia, Ali, Qurban, Ali, Muhsin, Khan, Muhammad Ajmal, Yasmin, Humaira, Khan, Bakhtawar, Amin, Aftab, Ahmad, Ajaz, Farid, Awais, Ullah, Amin, Butt, Sadia, and Ali, Qurban

Abstract

Background: Achyranthes bidentata Blume (Abb) is a renowned medicinal herb in traditional Chinese medicine. The molecular mechanism associated with the anti-diabetic potential of Abb is yet to be determined. In this study, we elucidated the in vivo and in vitro anti-diabetic potential of the methanolic leaf extract of Abb (Abb-ME) in alloxan-induced diabetic mice and HepG2 cells.Methods: Qualitative and quantitative phytochemical analysis was performed to identify the metabolites in the Abb-ME. Diabetes mellitus (DM) was induced in mice using alloxan monohydrate (150 mg/kg). Diabetic parameters (blood glucose, body weight, lipid profile, hematological indices, histopathological changes) were observed post Abb-ME administration. The in vitro study included glucose uptake assay and quantitative reverse transcription-PCR (qRT-PCR) analysis of glucose transporters 2 (GLUT2) gene expression in HepG2 cells.Results: Qualitative and quantitative phytochemical analysis revealed the presence of alkaloids, flavonoids (44.89 +/- 3.9 mg/g extract), carbohydrates, proteins, phenols (60.3 +/- 1.2 mg/g extract), and steroids. The results showed that the Abb-ME treatment group (500 mg/kg) exhibited significant (p < 0.05) reductions in blood glucose levels compared to the diabetic control and Glibenclamide group. Other diabetic parameters, such as body weights and lipid profiles, were significantly improved (p < 0.05) in the Abb-ME treated group (500 mg/kg) compared to the diabetic control mice. The altered hematological indices, including packed cell volume (PCV), hemoglobin (Hb), Red Blood Cell (RBC), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), Total White Blood Cell (TWBC), and White Blood Cell (WBC) differentials, observed in the diabetic control group were normalized after Abb-ME administration. These findings revealed that Abb-ME treatment effectively resolved several aberrant hematological indices associated with diabetes. Histopathological analysis of mi

Published
2023

127. Nitrate Reductase Drives Nutrition Control and Disease Resistance in Tomato (Solanum lycopersicumL.) Cultivars

Author

Kong, Mengmeng, Xu, Huilian, Ali, Qurban, Jing, Hairong, Wang, Fuli, Xu, Qicong, Li, Fenglan, and Shen, Yu

Abstract

Nitrate reductase plays an important role in preventing the occurrence of diseases and insect pests, which could rapidly reduce the accumulation of nitrate and induce the excess of soluble nitrogen compounds in plants. Tomato varieties “Myoko” (natural farming) and “Momotaro” (conventional chemical agriculture) were used in this study to show the positive correlation of the disease resistance and the enhanced nitrate reductase activity and the upregulated NR1, through the analysis of nitrate reductase genes, nitrate reductase activity. Disease resistance to early blight and mosaic virus was assessed, as was photosynthetic activity. Fruit yield and various biochemical parameters in fruit and leaves were determined. It was shown that Myoko F1 showed higher nitrate reductase activity compared to Momotaro, with significant differences seen in the expanding first leaf. This difference became more pronounced as the plants aged. Myoko and its parents exhibited higher NR1expression than Momotaro. A modified Gaussian function model was used to simulate the expression trend, revealing significant differences between the tomato lines. The expression level of the NR1and the daily average yield of tomato fruit during the growth period were simulated by the modified Gaussian curve equation (y= YMe−α(x−τ)^2+ YB(1 + βt)). Disease resistance to early blight was observed, with Myoko F1 showing higher resistance than Momotaro. The resistance was associated with higher nitrate reductase activity and NR1expression. And the fruit yield showed that Myoko F1 had slightly higher yield potential than Momotaro due to its disease resistance. Myoko F1 inherited yield heterosis from its parents. Nitrate and amino acid concentrations in leaves were lower in Myoko F1, consistent with its higher disease resistance, nitrate reductase activity, and NR1expression. At the same time, Myoko F1 exhibited higher concentrations of soluble sugars, organic acids, and vitamin C in tomato fruit compared to Momotaro, while nitrate concentration was lower. In addition, photosynthetic capacity (PC) was higher in Momotaro at the seedling stage but declined in the later fruit harvest period. Myoko and its parents maintained higher PC in the later stage, indicating better disease resistance and overall performance. In summary, Myoko F1’s prolonged optimal NR activity and NR1 expression, mediated by disease resistance, allow continued growth and yield despite biotic stressors. Further elucidating the connections between NR activity, senescence, and disease resistance will be valuable. Myoko F1 shows promise, but more work should explore its yield heterosis and suitability for fully natural, organic agriculture.

Published
2024
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128. Exogenous Melatonin Regulates Plant‐Disease Interaction by Inducing Maize Resistance and Decreasing the Pathogenicity of Fusarium graminearum.

Author

Kong, Mengmeng, Ali, Qurban, Jing, Hairong, Hussain, Amjad, Wang, Fuli, Liu, Xiaoyong, Gao, Xuewen, and Xu, Hui‐lian

Subjects
*MELATONIN, *PLANT enzymes, *PLANT diseases, *SALICYLIC acid, *FUSARIUM, *CORN, *CORN diseases
Abstract

Plants cannot avoid environmental challenges and are constantly threatened by diverse biotic and abiotic stresses. However, plants have developed a unique immune system to defend themselves against the invasion of various pathogens. Melatonin, N‐acetyl‐5‐methoxytryptamine has positive physiological effects in plants that are involved in disease resistance. The processes underlying melatonin‐induced pathogen resistance in plants are still unknown. The current study explores how melatonin regulates the plant‐disease interaction in maize. The results showed that 400 μM melatonin strongly reduced the disease lesion on maize stalks by 1.5 cm and corn by 4.0 cm caused by Fusarium graminearum PH‐1. Furthermore, after treatment with melatonin, the plant defense enzymes like SOD significantly increased, while POD and APX significantly decreased compared to the control. In addition, melatonin can also improve maize's innate immunity, which is mediated by melatonin treatments through the salicylic acid signaling pathway, and up‐regulate the defense‐associated expression of PR1, LOX1, OXR, serPIN, and WIPI genes in maize. Melatonin not only inhibits the disease in the maize stalks and corn, but also down‐regulates the deoxynivalenol (DON) production‐related expression of genes Tri1, Tri4, Tri5, and Tri6 in maize. Overall, this study sheds new light on the mechanisms by which melatonin regulates antioxidant enzymes and defense‐related genes involved in plant immunity to effectively suppress plant diseases. [ABSTRACT FROM AUTHOR]

Published
2023
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129. Plant-based products: Explore a way forward for mosquito’s management: A review.

Author

hanif, Kanwal, Hussain, Dilbar, Ranjha, Mazhar Hussain, Ali, Qurban, Aslam, Asad, Zubair, Muhammad, Saleem, Muhammad, Nazir, Tamsila, Asghar, Sabeen, and Akhtar, Muhammad Faheem

Subjects
RIFT Valley fever, PLANT extracts, VIRUS diseases, MOSQUITO-borne diseases, MOSQUITOES, WEST Nile virus
Abstract

Mosquitoes (Diptera: Culicidae) pose a serious threat to humans worldwide, known to transmit various pathogens that cause infectious diseases and arboviruses, which are viral diseases such as chikungunya, dengue, Rift Valley fever, yellow fever, malaria, elephantiasis, Murray Valley encephalitis, Japanese encephalitis, Saint Louis encephalitis virus, West Nile virus, Eastern equine encephalomyelitis virus, Highlands J virus, Everglades virus, and crosse encephalitis virus. The emergence of widespread insecticide resistance and the potential adverse environmental problems associated with synthetic insecticides have highlighted the need for alternative methods to control the spread of mosquito populations, making it an important research priority. Safe and innovative tools, such as plant-based repellents, have recently been implemented to enhance mosquito management strategies. Plant-based repellents play a crucial role in the development of natural products as alternatives to chemical control methods. Plant extracts and essential oils, which are biodegradable, target-specific, eco-friendly, and have potent effects against mosquitoes, are reviewed in this article. These plant-based repellents offer promising potential for effective mosquito management, providing a safer and environmentally friendly approach to control mosquito populations and reduce the spread of mosquito-borne diseases. Properly formulated and used in combination with other integrated vector management strategies, plant-based repellents can contribute to sustainable and effective mosquito control efforts. [ABSTRACT FROM AUTHOR]

Published
2023
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134. Hydropriming and Osmotic Priming Induce Resistance against Aspergillus niger in Wheat (Triticum aestivum L.) by Activating β-1, 3-glucanase, Chitinase, and Thaumatin-like Protein Genes

Author

Gul, Summia, primary, Hussain, Amjad, additional, Ali, Qurban, additional, Alam, Intikhab, additional, Alshegaihi, Rana M., additional, Meng, Qinglin, additional, Zaman, Wajid, additional, Manghwar, Hakim, additional, and Munis, Muhammad Farooq Hussain, additional

Published
2022
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135. Assessment of sublethal and transgenerational effects of spirotetramat, on population growth of cabbage aphid, Brevicoryne brassicae L. (Hemiptera: Aphididae)

Author

Iftikhar, Ayesha, primary, Hafeez, Faisal, additional, Aziz, Muhammad Asif, additional, Hashim, Muhammad, additional, Naeem, Afifa, additional, Yousaf, Hafiz Kamran, additional, Saleem, Muhammad Jawad, additional, Hussain, Sabir, additional, Hafeez, Muhammad, additional, Ali, Qurban, additional, Rehman, Muzammal, additional, Akhtar, Sumreen, additional, Marc, Romina Alina, additional, Syaad, Khalid M. Al, additional, Mostafa, Yassor Sabry, additional, and Saeed, Fatimah A. Al, additional

Published
2022
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139. Genome-Wide Identification and In Silico Analysis of ZF-HD Transcription Factor Genes in Zea mays L.

Author

Islam, Md. Abir Ul, primary, Nupur, Juthy Abedin, additional, Khalid, Muhammad Hayder Bin, additional, Din, Atta Mohi Ud, additional, Shafiq, Muhammad, additional, Alshegaihi, Rana M., additional, Ali, Qurban, additional, Kamran, Zuha, additional, Manzoor, Mujahid, additional, Haider, Muhammad Saleem, additional, Shahid, Muhammad Adnan, additional, and Manghwar, Hakim, additional

Published
2022
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140. WRKY transcription factors (TFs): Molecular switches to regulate drought, temperature, and salinity stresses in plants

Author

Khoso, Muneer Ahmed, primary, Hussain, Amjad, additional, Ritonga, Faujiah Nurhasanah, additional, Ali, Qurban, additional, Channa, Muhammed Malook, additional, Alshegaihi, Rana M., additional, Meng, Qinglin, additional, Ali, Musrat, additional, Zaman, Wajid, additional, Brohi, Rahim Dad, additional, Liu, Fen, additional, and Manghwar, Hakim, additional

Published
2022
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144. Land degradation resistance potential of a dry, semiarid region in relation to soil organic carbon stocks, carbon management index, and soil aggregate stability

Author

Ameer, Imran, primary, Kubar, Kashif Ali, additional, Ali, Qurban, additional, Ali, Shahmir, additional, Khan, Tamoor, additional, Shahzad, Khurram, additional, Riaz, Muhammad, additional, Shah, Zia‐ul‐hassan, additional, Rajpar, Inayatullah, additional, Ahmed, Muneer, additional, and Talpur, Khalid Hussain, additional

Published
2022
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146. Enhancement of nitrogen use efficiency through agronomic and molecular based approaches in cotton

Author

Chattha, Muhammad Sohaib, primary, Ali, Qurban, additional, Haroon, Muhammad, additional, Afzal, Muhammad Junaid, additional, Javed, Talha, additional, Hussain, Sadam, additional, Mahmood, Tahir, additional, Solanki, Manoj K., additional, Umar, Aisha, additional, Abbas, Waseem, additional, Nasar, Shanza, additional, Schwartz-Lazaro, Lauren M., additional, and Zhou, Lei, additional

Published
2022
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150. Phytochemical profiling and cytotoxic potential of Arnebia nobilis root extracts against hepatocellular carcinoma using in-vitro and in-silico approaches.

Author

Kiran, Asia, Altaf, Awais, Sarwar, Muhammad, Malik, Arif, Maqbool, Tahir, and Ali, Qurban

Subjects
PHYTOCHEMICALS, HEPATOCELLULAR carcinoma, CARDIAC glycosides, RADICAL anions, LIVER cancer, EXTRACTS
Abstract

Hepatocellular carcinoma is the fifth most prevalent cancer worldwide. The emergence of drug resistance and other adverse effects in available anticancer options are challenging to explore natural sources. The current study was designed to decipher the Arnebia nobilis (A. nobilis) extracts for detecting phytochemicals, in-vitro evaluation of antioxidative and cytotoxic potentials, and in-silico prediction of potent anticancer compounds. The phytochemical analysis revealed the presence of flavonoids, phenols, tannins, alkaloids, quinones, and cardiac glycosides, in the ethanol (ANE) and n-hexane (ANH) extracts of A. nobilis. ANH extract exhibited a better antioxidant potential to scavenge DPPH, nitric oxide and superoxide anion radicals than ANE extract, which showed better potential only against H2O2 radicals. In 24 h treatment, ANH extract revealed higher cytotoxicity (IC50 value: 22.77 µg/mL) than ANH extract (IC50 value: 46.74 µg/mL) on cancer (HepG2) cells without intoxicating the normal (BHK) cells using MTT assay. A better apoptotic potential was observed in ANH extract (49.10%) compared to ANE extract (41.35%) on HepG2 cells using the annexin V/PI method. GCMS analysis of ANH extract identified 35 phytocompounds, from which only 14 bioactive compounds were selected for molecular docking based on druggability criteria and toxicity filters. Among the five top scorers, deoxyshikonin exhibited the best binding affinities of − 7.2, − 9.2, − 7.2 and − 9.2 kcal/mol against TNF-α, TGF-βR1, Bcl-2 and iNOS, respectively, followed by ethyl cholate and 2-Methyl-6-(4-methylphenyl)hept-2-en-4-one along with their desirable ADMET properties. The phytochemicals of ANH extract could be used as a promising drug candidate for liver cancer after further validations. [ABSTRACT FROM AUTHOR]

Published
2023
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