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Introduction: Phosphorus (P) readily forms insoluble complexes in soil, thereby inhibiting the absorption and utilization of this essential nutrient by plants. Phosphorus deficiency can significantly impede the growth of forage grass. While Trichoderma viride (T. viride) has been recognized for promoting the assimilation of otherwise unobtainable nutrients, its impact on P uptake remains understudied. Consequently, it is imperative to gain a more comprehensive insight into the role of T. viride in facilitating the uptake and utilization of insoluble P in forage grass. Methods: This research explored the influence of T. viride inoculation on P absorption and the growth of Chloris virgata (C. virgata) across various P sources. We treated plants with control P (P), tricalcium phosphate (TCP), calcium phytate (PHY), and low P (LP), with and without T. viride inoculation (P+T, TCP+T, PHY+T, LP+T). We analyzed photosynthesis parameters, growth indices, pigment accumulation, P content, leaf acid phosphatase activity. Results: Results demonstrated that T. viride inoculation alleviated inhibition of photosynthesis, reduced leaf acid phosphatase activity, and enhanced growth of C. virgata in the presence of insoluble P sources. Additionally, T. viride inoculation enabled the plants to extract more available P from insoluble P sources, as evidenced by a substantial increase in P content: shoot P content surged by 58.23 to 59.08%, and root P content rose by 55.13 to 55.2%. Biomass P-use efficiency (PUE) declined by 38% upon inoculation with T. viride compared to the noninoculated insoluble P sources, paralleled by a reduction in photosynthetic P-use efficiency (PPUE) by 26 to 29%. Inoculation under insoluble P sources further triggered a lower allocation to root biomass (25 to 26%) and a higher investment in shoot biomass (74 to 75%). However, its application under low P condition curtailed the growth of C. virgata. Discussion: Our results suggest that T. viride inoculation represents an innovative approach for plants to acquire available P from insoluble P sources, thereby promoting growth amid environmental P limitations. This insight is crucial for comprehending the synergy among forage grass, P, and T. viride. [ABSTRACT FROM AUTHOR]

Two potent greenhouse gases that are mostly found in agricultural soils are methane and nitrous oxide. Therefore, we investigated the effect of different moisture regimes on microbial stoichiometry, enzymatic activity, and greenhouse gas emissions in long-term paddy soils. The treatments included a control (CK; no addition), chemical fertilizer (NPK), and NPK + cattle manure (NPKM) and two moisture regimes such as 60% water-filled pore spaces (WFPS) and flooding. The results revealed that 60% water-filled pore spaces (WFPS) emit higher amounts of N2O than flooded soil, while in the case of CH4 the flooded soil emits more CH4 emission compared to 60% WFPS. At 60% WFPS higher N2O flux values were recorded for control, NPK, and NPKM which are 2.3, 3.1, and 3.5 µg kg−1, respectively. In flooded soil, the CH4 flux emission was higher, and the NPKM treatment recorded the maximum CH4 emissions (3.8 µg kg−1) followed by NPK (3.2 µg kg−1) and CK (1.7 µg kg−1). The dissolved organic carbon (DOC) was increased by 15–27% under all flooded treatments as compared to 60% WPFS treatments. The microbial biomass carbon, nitrogen, and phosphorus (MBC, MBN, and MBP) significantly increased in the flooded treatments by 8–12%, 14–21%, and 4–22%, respectively when compared to 60% WFPS. The urease enzyme was influenced by moisture conditions, and significantly increased by 42–54% in flooded soil compared with 60% WFPS while having little effect on the β-glucosidase (BG) and acid phosphatase (AcP) enzymes. Moreover DOC, MBC, and pH showed a significant positive relationship with cumulative CH4, while DOC showed a significant relationship with cumulative N2O. In the random forest model, soil moisture, MBC, DOC, pH, and enzymatic activities were the most important factors for GHG emissions. The PLS-PM analysis showed that soil properties and enzymes possessed significantly directly impacted on CH4 and N2O emissions, while SMB had indirect positive effect on CH4 and N2O emissions. [ABSTRACT FROM AUTHOR]

5′-Phosphorylation of nucleosides is a reaction as important in nature and in industry as it is cumbersome to be performed. Whilst chemical phosphorylation relies on the use of harsh reagents, solvents, and conditions, as well as on the need for protection–deprotection steps, biocatalysis can be a tool to achieve one-step phosphorylation reactions, which are selective, protecting group-free, and occurring under mild and sustainable conditions. In this work, the wild-type non-specific acid phosphatase from Morganella morganii (PhoC-Mm) was expressed, purified, and used for the synthesis of inosine 5′-monophosphate (IMP), an important food additive, by using pyrophosphate (PPi) as an inexpensive phosphate donor in a fully aqueous medium at 30 °C. Via the fine-tuning of the reaction set-up taking into account the type of buffer, amount of PPi, mode/time of PPi addition, and enzyme and substrate concentration, PhoC-Mm could be used for catalyzing the phosphorylation of inosine (I) to IMP in a good yield and high purity (62% yield). The catalysis of the hydrolytic reaction direction, which is the primary function of phosphatases in nature, was here reversed to a certain extent by a reaction engineering approach, without the need for protein engineering strategies. [ABSTRACT FROM AUTHOR]

In an era of striking climate change, increased marine temperature severely affects aquatic animals and cause significant ecological and socioeconomic impacts. At present, there is little information about the effects of heat stress on the physiology of greater amberjack (Seriola dumerili). This study explores the mechanisms whereby juvenile greater amberjacks cope with heat stress. An increase in water temperature (25 to 28°C and 31°C) changed the activity levels of enzymes responsible for antioxidant defense, immune function, and energy metabolism. When water temperatures increased from 25 to 28°C and 31°C, the levels of malondialdehyde (MDA) and glucose (GLU) in the greater amberjacks increased, suggesting that the fish suffered oxidative stress. During the early stage of heat stress, the levels of triglycerides (TG) and glutathione (GSH) and the activities of superoxide dismutase (SOD), catalase (CAT), alkaline phosphatase (ALP), acid phosphatase (ACP), lysozyme (LYZ), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and succinate dehydrogenase (SDH) increased significantly suggesting that juvenile greater amberjacks adapt to heat stress by enhancing their antioxidant defense, energy metabolism, and immune defense. However, long‐term exposure to heat stress decreased the levels of TG and GSH; decreased the activities of SOD, CAT, ALP, ACP, LYZ, and AST; and increased the levels of MDA and GLU, suggesting that the energy metabolism and immune function of juvenile greater amberjack S. dumerilis are suppressed by oxidative damage. This study provides insights into the physiological adaptation of juvenile greater amberjacks to heat stress. [ABSTRACT FROM AUTHOR]

Background: The number of adult orthodontic patients is increasing, and studies have shown that autophagy is involved in regulating orthodontic tooth movement and plays an important role in aging-related changes. Therefore, we aimed to explore the role of autophagy in aging-related changes during orthodontic tooth movement by establishing a rat orthodontic tooth movement model. Methods: Forty-five 6-week-old and sixty-five 8-month-old male Sprague–Dawley rats were selected to represent adolescents and adults and establish orthodontic tooth movement model. They were sacrificed on days 0,1,3,7 and 14. Immunohistochemistry, immunofluorescence and tartrate resistant acid phosphatase (TRAP) staining were applied to measure the expression level of osteogenesis, autophagy, aging factors and osteoclast number in periodontal membrane of left upper first molar during orthodontic tooth movement. Then, we regulated the autophagy level by injecting autophagy activator rapamycin during orthodontic tooth movement and measured these factors and tooth movement distance by micro-computed tomography. Results: Aging factor levels in the periodontal membrane were higher in adult rats than in adolescent rats and the autophagy factor levels were lower. The levels of osteogenic factors were lower on the tension side in adult rats than in adolescent rats. The peak osteoclast number on the pressure side occurred later in adult rats than in adolescent rats. The injection of rapamycin increased autophagy, accelerated orthodontic tooth movement in adult rats, and reduced the levels of aging factors. The levels of osteogenic factors were higher and reached those in adolescent rats at some time points. The number of osteoclasts increased significantly in the early stage. Conclusions: Autophagy may play a substantial role in regulating aging-related changes in orthodontic tooth movement. [ABSTRACT FROM AUTHOR]

A promising method of regenerative medicine is the saturation of allografts with platelet-rich plasma (PRP). Objective. To evaluate the course of metabolic processes after filling a hole defect in the distal metaphysis of the femur with allogeneic bone implants in conditions of additional local administration of allogeneic PRP. Methods. In the model of a hole defect in white rats with the filling of the defect with an allograft, as well as with additional local stimulation of PRP on the 7th day; on the 3rd and 7th days and on the 1st, 3rd and 7th days in blood serum, the content of glycoproteins (GP), chondroitin sulfates (CST), total protein (TP), calcium (Ca), activity alkaline phosphatase (AlP) and acid phosphatase (AcP). The results. 14 day. In the 3-month rats, under one stimulation, an increase in TP and Ca, a decrease in AcP was observed, with two stimulations there was a 1.11 times smaller GP, 1.13 times larger TP and 1.43 times Ca compared to those in rats without stimu-lation. During three stimulations, the GP was 1.24 times lower than that in animals without stimulation. In the 12-month rats in comparison with the data of rats without stimulation, 1.15 times higher GP, Ca, AlP activity, and 1.44 times more AcP were noted. 28 days of the 3-month rats for one injection exceeded the data of animals without stimulation by 1.34 times for GP, and were inferior to them by 1.31 times for AcP. In the 12-month rats, compared to these animals without stimulation, with three injections, a 1.19 times greater TP was noted. 90 d. In the 3-month rats for one injection showed 1.24 times less CST with 1.28 times lower AlP compared to data from rats without stimulation. 12-month rats exceeded the data of the group without stimulation by 1.43 times for AlP. Conclusions. In rats with an alloimplant (especially at 12 months), an increase in connective tissue formation markers and a decrease in LF activity were observed. Filling the defect with an alloimplant led to an increase in inflammation indicators and an increase in markers of bone tissue formation. [ABSTRACT FROM AUTHOR]

Simple Summary: Analyzing the immune mechanism of sea urchins against pathogens is the basis of disease-resistant breeding. When faced with pathogen invasion, the phagocytosis of sea urchin coelomocytes plays a major immune role. However, it is unclear how can phagocytosis-related immune indices change can help sea urchins resist disease. To answer this question, this study compared the differences in immune indices related to phagocytosis between susceptible and disease-resistant sea urchins after pathogen infection. We found that the apoptosis and necrosis rate of phagocytes, phagocytic index, ACP, ROS and T-AOC may be used as indicators of disease resistance in sea urchins. Disease resistance standards in immune indices can be summarized as phagocytosis increases greatly in the early infection stage and decreases timely to a normal level after killing the pathogen in a short period. The results of this paper can provide important references for disease-resistant breeding of sea urchins. To screen for immune indicators closely related to disease resistance, two species of sea urchin susceptible to black mouth disease (Strongylocentrotus intermedius, S. intermedius ♀ × Heliocidaris crassispina ♂) and three species of sea urchin resistant to black mouth disease (H. crassispina, H. crassispina ♀ × S. intermedius ♂ and Mesocentrotus nudus) were artificially infected with the black mouth pathogen Vibrio echinoideorum. The phagocytosis-related immune indices of the five sea urchin species were compared at different time points post-infection. The results demonstrated that the parameters such as apoptotic rate of phagocytes, mean contribution value (MCV) of single effective phagocyte on Acid Phosphatase (ACP), Reactive Oxygen Species (ROS), and Total Antioxidant Capacity (T-AOC) of the five sea urchin species first increased and then decreased after infection. The key time points were 3 h to 6 h and 48 h post-infection when the black mouth disease-resistant and susceptible sea urchins demonstrated differences. At 3 h or 6 h post-infection, the up-regulation folds in MCV of ACP, ROS and T-AOC of black mouth disease-resistant sea urchins were considerably higher than that of the susceptible sea urchins. At 6 h post-infection, the apoptosis rate and the phagocytic index (PI) of the black mouth disease-resistant sea urchins were significantly higher than those of the susceptible sea urchins (p < 0.05). At 48 h post-infection, the necrosis rate of phagocytes, MCV of ACP and MCV of ROS of the black mouth disease-resistant sea urchins were significantly lower than those of the susceptible sea urchins (p < 0.05). The apoptosis and necrosis rate of phagocytes, PI, and MCV on ACP, ROS may be used as indicators of disease resistance in sea urchins. Disease resistance standards in immune indices can be summarized as phagocytosis increases greatly in the early infection stage and decreases timely to a normal level after killing the pathogen in a short period. [ABSTRACT FROM AUTHOR]

Recently, interest in fish byproduct-derived bioactive components as potential ingredients for treating and preventing osteoporosis is increasing. In this study, we investigated the antiosteoporotic effects of mackerel Scomber japonicus extracts in MC3T3-E1 preosteoblasts in vitro and ovariectomized rats in vivo. Scomber japonicus neutrase-assisted protein hydrolysate (SJNH) treatment enhanced alkaline phosphatase (ALP) activity and osteoprotegerin (OPG) level, and attenuated receptor activator of nuclear factor κB ligand (RANKL) level in MC3T3-E1 cells. In an osteoporotic animal model, ovariectomized (OVX) rats were administered SJNH (200 mg/kg/day) for 8 weeks. SJNH prevented OVX-induced bone mineral density (BMD) loss and restored bone structural compartment loss based on micro-computed tomography (CT). In addition, SJNH administration normalized bone remodeling by regulating bone regeneration and resorption-related proteins such as ALP, osteocalcin (OC), and tartrate-resistant acid phosphatase 5b (TRACP-5b) as well as calcium contents in serum. Taken together, our findings suggest that SJNH can promote antiosteoporotic effects both in vitro and in vivo. Thus, SJNH might be a potent therapeutic agent or functional food ingredient for preventing osteoporosis. [ABSTRACT FROM AUTHOR]

AIM: The study aimed to explore the effect of curcumin (Cur) on lipopolysaccharide (LPS)-induced RAW264. 7 cell-derived osteoclasts, together with its underlying mechanism. METHODS: An osteoclast model was established by treating RAW264. 7 cells with LPS. The viability of the cells was assessed by CCK-8 assays and osteoclast formation was evaluated by tartrate-resistant acid phosphatase (TRAP) activity. The levels of reactive oxygen species (ROS), Fe2+, glutathione (GSH), and malondialdehyde (MDA) were examined by biochemical assays. Mitochondrial morphology was assessed by transmission electron microscopy. The mRNA and protein levels of p53, glutathione peroxidase 4 (GPX4), and solute carrier family 7 member 11 (SLC7A11) were determined by RT-qPCR and Western blot, respectively. RESULTS: Treatment with LPS successfully induced osteoclasts formation in RAW264. 7 cells. The TRAP results showed that compared with the LPS-treated group, the number of osteoclasts and TRAP activity in the curcumin-treated group decreased dose-dependently (P<0. 01). Compared with the control group, the LPS+Erastin group showed significantly increased TRAP activity (P<0. 01), while after curcumin treatment, the TRAP activity declined in a dose-dependent manner (P<0. 01). The results of the biochemical tests showed that compared with the control group, the LPS+Erastin group had significantly elevated levels of ROS, Fe2+, and MDA, while the GSH level was significantly reduced (P< 0. 01, and compared with the LPS+Erastin group, the ROS, Fe2+, and MDA levels in the curcumin group decreased (P< 0. 01) and GSH levels increased (P<0. 01). These effects were all dose-dependent. Transmission electron microscopy showed that compared with the LPS group, the LPS+Erastin group had reduced mitochondrial cristae and increased membrane density, while after treatment with curcumin, both these effects were reversed. The RT-qPCR and Western blot results showed that compared with the control group, the mRNA and protein levels of p53 in the LPS+Erastin group were significantly increased, while those of of SLC7A11 and GPX4 were significantly reduced (P<0. 01). After curcumin treatment, the p53 mRNA and protein levels were reduced while the levels of SLC7A11 and GPX4 were increased (P<0. 01). CONCLUSION: Curcumin can inhibit lipopolysaccharide-induced differentiation of RAW264. 7 cells into osteoclasts, and its mechanism may be related to the inhibition of the p53/SLC7A1 1/GPX4 signaling pathway. [ABSTRACT FROM AUTHOR]

Alfalfa is a perennial herbaceous forage legume that is significantly and adversely affected by monocropping. Crop rotation is the most effective measure to overcome continuous cropping obstacles. However, the mechanisms of how bacterial communities are affected and the potential links between these effects and cropping systems remain poorly understood. Based on a long-term field experiments with continuous alfalfa and forage crops with alfalfa rotation in the black soil region of the western Songnen Plain in Northeast China, the alterations in soil bacterial community structure using high-throughput sequencing of the 16S rRNA gene and soil chemical properties and enzyme activities were analyzed. The alfalfa–forage oats–silage maize–alfalfa and alfalfa–silage maize–forage oats–alfalfa system significantly increase the levels of total phosphorus and available phosphorus, and promote the activities of acid phosphatase, β-glucosidase, leucine aminopeptidase, and N-acetyl-β-glucosaminidase in comparison to continuous alfalfa. While alfalfa crop rotation did not affect the α-diversity of soil bacteria, it significantly altered the bacterial community composition and structure. Some key taxa are significantly enriched in the crop rotation system soils, including Bacillus, Sphingobium, Paenibacillus, Hydrogenispora, Rubrobacter, Haliangium, and Rubellimicrobium. Additionally, crop rotation with alfalfa increased the stability and complexity of the soil bacterial co-occurrence network. Based on our findings, we recommend promoting the alfalfa–forage oats–silage maize–alfalfa and alfalfa–silage maize–forage oats–alfalfa rotation systems as ideal practices for overcoming the challenges associated with continuous cropping of alfalfa. These systems not only enhance soil nutrient content and enzyme activities but also foster a beneficial microbial community, ultimately improving soil functionality and crop performance. [ABSTRACT FROM AUTHOR]

Research was carried out on the impact of long-term use of cattle manure (30 t ha−1 FYM) and various doses of N (0, 40, 60 and 120 t ha−1) in the form of ammonium nitrate on the following soil parameters: salinity, hydrolytic acidity, total exchangeable base cations, cation exchange capacity, degree of base saturation of the sorption complex, total organic carbon and total nitrogen content, dissolved organic matter, fractional composition of organic matter and content of bioavailable macroelements: phosphorus, potassium and magnesium in the soil of a multi-year static field experiment. The activities of dehydrogenases, catalase, alkaline and acid phosphatase and proteases were also tested. A significant effect of FYM and N fertilisation on the content of bioavailable macroelements was found. The application of manure at a dose of 30 t ha−1 mitigated the negative effects of the application of N at a dose of 120 kg ha−1. A higher content of total organic carbon (8.42 g kg−1) and humic acid fraction (1761 mg kg−1) and higher values of the CHA/CFA ratio (0.79; parameters that are indicators of soil quality) were found in the soil fertilised with manure compared to the soil without manure added (TOC—7.00 g kg−1; CHAs—1285 mg kg−1; CHAs/CFAs 0.66). The activity of the tested enzymes was also significantly determined by the applied fertilisation. Enzyme activity was highest in the soil to which manure had been applied. Nitrogen fertilisation varied in its impact on the activity of enzymes according to the specifics of each enzyme. The content of humic acids and CHA/CFA values correlated positively with the content of soil minerals and the activity of dehydrogenases, catalase, alkaline and acid phosphatase and GMea and TEI indices. Dehydrogenases and acid phosphatase can be considered enzymes that take part in transforming organic matter towards the formation of FAs. [ABSTRACT FROM AUTHOR]

Drought presents a significant abiotic stress that threatens crop productivity worldwide. Rhizosphere bacteria play pivotal roles in modulating plant growth and resilience to environmental stresses. Despite this, the extent to which rhizosphere bacteria are instrumental in plant responses to drought, and whether distinct cassava (Manihot esculenta Crantz) varieties harbor specific rhizosphere bacterial assemblages, remains unclear. In this study, we measured the growth and physiological characteristics, as well as the physical and chemical properties of the rhizosphere soil of drought-tolerant (SC124) and drought-sensitive (SC8) cassava varieties under conditions of both well-watered and drought stress. Employing 16S rDNA high-throughput sequencing, we analyzed the composition and dynamics of the rhizosphere bacterial community. Under drought stress, biomass, plant height, stem diameter, quantum efficiency of photosystem II (Fv/Fm), and soluble sugar of cassava decreased for both SC8 and SC124. The two varieties' rhizosphere bacterial communities' overall taxonomic structure was highly similar, but there were slight differences in relative abundance. SC124 mainly relied on Gamma-proteobacteria and Acidobacteriae in response to drought stress, and the abundance of this class was positively correlated with soil acid phosphatase. SC8 mainly relied on Actinobacteria in response to drought stress, and the abundance of this class was positively correlated with soil urease and soil saccharase. Overall, this study confirmed the key role of drought-induced rhizosphere bacteria in improving the adaptation of cassava to drought stress and clarified that this process is significantly related to variety. [ABSTRACT FROM AUTHOR]

In order to improve the phosphorus(P)deficiency in the soil of Eucalyptus plantations in subtropical China, we used biochar(BC)as a soil amendment to investigate the soil of Eucalyptus plantations(15 years old). Meanwhile, we added the different amounts [0(CK), 2%, 5%, 10% and 20%] of BC through the laboratory culture experiment to evaluate the effects of different amounts of BC on P components and transformation in soil and its relationship with soil physicochemical properties. The results were as follows: (1) Compared to CK, the nitrate nitrogen(NO3--N), total phosphorus(TP), microbial biomass phosphorus(MBP)and pH values were significantly increased at 20% BC addition, respectively(P<0. 05), the MBP and pH values were significantly increased at 2%, 5%, and 10% BC addition, respectively(P<0. 05), while no marked difference was found in other soil physicochemical properties. (2)Compared to CK, the labile P(LP)was significantly increased at 2% of BC addition(P<0. 05), the available P(AP)and LP were significantly increased at 5% and 10% of BC additions, respectively(P<0. 05). Moreover, the AP, LP and occluded P(OP)were significantly increased at 20% BC addition(P<0. 05), whereas the moderately available P(MP)had no significant change under the four BC additions. (3)The total activities of β-glucosidase(BG), β-N-acetylglucosaminidase(NAG), L-leucine aminopeptidase(LAP), and acid phosphatase(ACP)involved in C, N and P transformations increased significantly at 10% and 20% BC additions than those in CK(P<0. 05). (4)Correlation analysis showed that ln(BG)and ln(NAG+LAP)were positively correlated with ln(ACP), respectively(P<0. 05). In addition, redundancy analysis(RDA)indicated that pH, TN and TP appeared to be the primary drivers of variations in soil P components of Eucalyptus plantations. Furthermore, structural equation model(SEM)revealed that the pH, C : P and N : P of soil were the most critical factors driving P transformation. In conclusion, our findings suggest that different amounts of BC improve the enzyme activities related to C, N cyclings by affecting soil physicochemical properties and improving the P supply potential of soil in Eucalyptus plantations. Notably, 20% BC addition had the optimum effect. This study provides critical theoretical guidance for gaining knowledge on soil nutrient management in Eucalyptus plantations and facilitating the sustainable development of forests in subtropical China. [ABSTRACT FROM AUTHOR]

Organic phosphorus(Po)is an important part of soil phosphorus reservoir. In order to investigate the effects of soil aggregate Po distribution properties in Pinus massoniana plantations after close-to-nature transformation, the pure P. massoniana plantations(PP)and P. massoniana-broad-leaved mixed plantations(CP)after close-to-nature transformation were investigated in southern subtropical China. Soil samples collected from 1-10 cm were screened into >2 mm, 0. 25-2 mm and <0. 25 mm three-part aggregates using the dry-sieving method. The Po fraction, microbial biomass phosphorus(MBP)content and acid phosphatase(ACP)activity in the bulk soil and aggregates were measured to understand the distribution of the Po fraction and its drivers. The results were as follows: (1)Soil Po fractions changed in CP compared to PP, with highly recalcitrant organic phosphorus(HRO-P)and moderately labile organic phosphorus(MLO-P)significantly greater in the bulk soil and soil aggregates than in PP(P<0. 05), while labile organic phosphorus(LO-P)and moderately recalcitrant organic phosphorus(MRO-P)were not significantly different in CP and PP. In addition, there was no consistent pattern of change in Po fractions in the bulk soil and soil aggregates between PP and CP. (2)The size of the proportion of each form of Po in PP was HRO-P>MRO-P>MLO-P>LO-P, while CP was HRO-P>MLO-P>MRO-P>LO-P. (3)The MBP content and ACP activity in CP were significantly greater than that of PP in the bulk soil and soil aggregates, and there was a tendency for the ACP activity to increase with the decrease in the size of aggregates. (4)Redundancy analysis revealed that soil available phosphorus(AP), soil aggregate mean weight diameter(MWD), MBP and total nitrogen(TN)were the main drivers of soil Po fractions. In conclusion, the close-to-nature transformation is beneficial to the accumulation and transformation of soil phosphorus in P. massoniana plantation. This study provides a theoretical reference for the improvement of soil quality and productivity in P. massoniana plantations. [ABSTRACT FROM AUTHOR]

A colorimetric analysis platform has been successfully developed based on FeCo-NC dual-atom nanozyme (FeCo-NC DAzyme) for the detection of organophosphorus pesticides (OPPs). The FeCo-NC DAzyme exhibited exceptional oxidase-like activity (OXD), enabling the catalysis of colorless TMB to form blue oxidized TMB (oxTMB) without the need for H2O2 involvement. By combining acid phosphatase (ACP) hydrolase with FeCo-NC DAzyme, a "FeCo-NC DAzyme + TMB + ACP + SAP" colorimetric system was constructed, which facilitated the rapid detection of malathion. The chromogenic system was applied to detect malathion using a smartphone-based app and an auxiliary imaging interferogram device for colorimetric measurements, which have a linear range of 0.05–4.0 µM and a limit of detection (LOD) as low as 15 nM in real samples, comparable to UV-Vis and HPLC-DAD detection methods. Overall, these findings present a novel approach for convenient, rapid, and on-site monitoring of OPPs. [ABSTRACT FROM AUTHOR]

Aging‐related bone loss is driven by various biological factors, such as imbalanced bone metabolism from decreased osteoblast and increased osteoclast activities. Various transcriptional and post‐transcriptional factors increase osteoclast activity with aging; however, studies regarding the post‐translational regulators of osteoclast activity are still limited. The ubiquitin E3 ligase Pellino‐1 is a well‐known post‐translational regulator of inflammation. However, how Pellino‐1 expression regulation affects osteoclast differentiation remains unclear. This study determined that Pellino‐1 levels are reduced in bone marrow monocytes (BMMs) from 40‐week‐old mice compared to 4‐week‐old mice. Interestingly, conditional Knockout (cKO) of Pellino‐1 in 6‐week‐old mice resulted in decreased bone mass, reduced body size, and lower weight than in Pellino‐1 floxed mice; however, these differences are not observed in 20‐week‐old mice. The increased number of tartrate‐resistant acid phosphatase (TRAP)‐positive cells and serum levels of C‐terminal telopeptides of type I collagen, a marker of bone resorption, in 6‐week‐old Pellino‐1 cKO mice implied a connection between Pellino‐1 and the osteoclast population. Enhanced TRAP activity and upregulation of osteoclast genes in BMMs from the cKO mice indicate that Pellino‐1 deletion affects osteoclast differentiation, leading to decreased bone mass and heightened osteoclast activity. Thus, targeting Pellino‐1 could be a potential gene therapy for managing and preventing osteoporosis. [ABSTRACT FROM AUTHOR]

In recent times, there has been growing attention towards exploring the nutritional and functional aspects of potato protein, along with its diverse applications. In the present study, we examined the anti‐osteoclast properties of potato protein hydrolysate (PP902) in vitro. Murine macrophages (RAW264.7) were differentiated into osteoclasts by receptor activator of nuclear factor‐κB ligand (RANKL), and PP902 was examined for its inhibitory effect. Initially, treatment with PP902 was found to significantly prevent RANKL‐induced morphological changes in macrophage cells, as determined by tartrate‐resistant acid phosphatase (TRAP) staining analysis. This notion was further supported by F‐actin analysis using a confocal microscope. Furthermore, PP902 treatment effectively and dose‐dependently down‐regulated the expression of RANKL‐induced osteoclastogenic marker genes, including TRAP, CTR, RANK, NFATc1, OC‐STAMP, and c‐Fos. These inhibitory effects were associated with suppressing NF‐κB transcriptional activation and subsequent reduced nuclear translocation. The decrease in NF‐κB activity resulted from reduced activation of its upstream kinases, including I‐κBα and IKKα. Moreover, PP902 significantly inhibited RANKL‐induced p38MAPK and ERK1/2 activities. Nevertheless, PP902 treatment prevents RANKL‐induced intracellular reactive oxygen species generation via increased HO‐1 activity. The combined antioxidant and anti‐inflammatory effects of PP902 resulted in significant suppression of osteoclastogenesis, suggesting its potential as an adjuvant therapy for osteoclast‐related diseases. [ABSTRACT FROM AUTHOR]

The efficiency of soil phosphorus (P) mobilization and uptake by plants depends on a complex combination of factors, including plant P acquisition strategies and soil P availability. The objective of this study was to assess and compare the capabilities of three legume species (blue lupin (Lupinus angustifolius L.), faba bean (Vicia faba L.), and chickpea (Cicer arietinum L.)), which may be used as green manures in temperate crop systems to acquire P from a soil with different levels of plant‐available P. Three cycles of each legume were grown in a glasshouse over a 6 month period in the same soil type with high (Olsen P: 47 mg kg−1) and low (Olsen P: 9 mg kg−1) levels of plant‐available P. Measurements included above—and below—ground plant biomass and P uptake, in addition to determination of acid and alkaline phosphomonoesterase activities, microbial P, and P fractions in soil at the end of the experiment. In both soils, plant biomass, P uptake, and microbial P were all higher under faba bean compared to blue lupin and chickpea (p <.05). In the low‐P soil, faba bean increased alkaline phosphomonoesterase activity (p <.05). Significant depletion of inorganic P in the soluble (46%–69%), labile (29%–42%), and moderately labile (15%–16%) pools and increase of organic P in the labile (13%–18%) and total (7%–13%) pools occurred under faba bean compared with blue lupin, while changes under chickpea were between those determined for faba bean and blue lupin (p <.05). The findings of this study indicated that inclusion of faba bean green manure may have the potential to improve overall P use efficiency by enhancing mobilization of labile soil inorganic P, although further research is required to investigate mobilization of more stable forms of soil legacy P and quantify the potential of faba bean as a green manure crop under field conditions. [ABSTRACT FROM AUTHOR]

The ubiquitous use of insecticides leads to detrimental effects on non)target organisms due to accidental exposure. Neonicotinoid insecticides are popularly used worldwide for their high affinity for arthropod nicotinic receptors which effectively kill insect pests. Low affinity towards vertebrate nicotinic receptors, make them safer as compared to traditional insecticides. Recent studies demonstrated that neonicotinoid exposure can cause some toxicity in vertebrates including humans. Zebrafish is one of the popular model organisms to study ecotoxicity. This is the first study on adult zebrafish to report the effect of novel neonicotinoid, Clothianidin on mortality, liver antioxidant stress profile, liver function profile and brain acetylcholinesterase (AChE). Observations were made over two treatment periods, 96 hours, and 21 days, in five groups exposed to varying concentrations of Clothianidin viz. 30mg/L, 50mg/L, 70mg/L, 90mg/L, 110mg/L and a control. Although no mortality was observed, Clothianidin exposure led to increased activity of superoxide dismutase (SOD) enzyme, lipid peroxidation and decreased catalase (CAT), glutathione-S-transferase (GST). Treated groups also showed increased concentrations of liver enzyme alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and acid phosphatase (ACP). These results indicate that Clothianidin causes disturbances in the hepatic function. A prominent decreasing trend observed in brain AChE shows that Clothianidin inhibits AChE in adult zebrafish. Further investigations on DNA damage and gene expression studies could be conducted to understand the exact mechanism of action of Clothianidin at molecular level. [ABSTRACT FROM AUTHOR]

Background: Understanding human stem cell differentiation into osteoblasts and osteoclasts is crucial for bone regeneration and disease modeling. Numerous morphological techniques have been employed to assess this differentiation, but a comprehensive review of their application and effectiveness is lacking. Methods: Guided by the PRISMA framework, we conducted a rigorous search through the PubMed, Web of Science and Scopus databases, analyzing 254 articles. Each article was scrutinized against pre-defined inclusion criteria, yielding a refined selection of 14 studies worthy of in-depth analysis. Results: The trends in using morphological approaches were identified for analyzing osteoblast and osteoclast differentiation. The three most used techniques for osteoblasts were Alizarin Red S (mineralization; six articles), von Kossa (mineralization; three articles) and alkaline phosphatase (ALP; two articles) followed by one article on Giemsa staining (cell morphology) and finally immunochemistry (three articles involved Vinculin, F-actin and Col1 biomarkers). For osteoclasts, tartrate-resistant acid phosphatase (TRAP staining) has the highest number of articles (six articles), followed by two articles on DAPI staining (cell morphology), and immunochemistry (two articles with VNR, Cathepsin K and TROP2. The study involved four stem cell types: peripheral blood monocyte, mesenchymal, dental pulp, and periodontal ligament. Conclusion: This review offers a valuable resource for researchers, with Alizarin Red S and TRAP staining being the most utilized morphological procedures for osteoblasts and osteoclasts, respectively. This understanding provides a foundation for future research in this rapidly changing field. [ABSTRACT FROM AUTHOR]

Pinus taeda plantations in subtropical regions of South America commonly occur on highly weathered soils of low natural fertility, especially phosphorus (P). Thus, phosphate fertilization is necessary in Pinus. The proposition of critical levels (CL) and sufficiency ranges (SR) of P in needles and soil can assist forest managers to decide the use of phosphate fertilizers, reducing the cost of production and the potential for contamination. The study aimed to propose critical levels and sufficiency ranges of phosphorus in young Pinus taeda submitted to phosphate fertilization in southern Brazil. The parameters height, stem diameter, P concentration in needles, soil available P, electron transport rate (ETRm), maximum potential quantum yield of PSII (Fv/Fm), initial (Fo) and maximum fluorescence (Fm), chlorophyll a (Chl a), b (Chl b) and total (Chl total) concentration, and acid phosphatase (APase) activity were evaluated. Productivity factor, CL and SR of P in needles and soil were calculated. The CL of P in needles in relation to relative productivity factor was 1.5 g P kg− 1. The CL of P for photosynthetic parameters were 1.5 and 1.7 g P kg− 1 for Chl b and ETRm, respectively. CL of P in soil was 2.3 mg P dm− 3. The results may contribute to appropriate recommendations for phosphate fertilizer, reducing production costs and possible P–fertilizer losses in Pinus taeda plantations. [ABSTRACT FROM AUTHOR]

The booming aquaculture industry has created a strong demand for fishmeal and increased environmental pressures. Spirulina, as a potential alternative to fishmeal, has been shown to have growth-promoting and animal health-enhancing properties. In this study, 600 large spiny loaches, divided into five experimental groups, F0, F1, F2, F3, and F4, were reared for 10 weeks using Spirulina platensis powder (SPP) as a substitute for 0%, 5%, 10%, 15%, and 20% of fishmeal, respectively. The results of intestinal physiological indexes showed that superoxide dismutase was lower than F0 in all treatment groups, and the activity of F3 was significantly lower than F0 (p < 0.05). The activity of malondialdehyde was significantly higher than that of F0 in all groups except F3 (p < 0.05). The addition of SPP also led to a decrease in the activity of acid phosphatase in the intestine, which was significantly lower in all treatment groups compared to the F0 group (p < 0.05). The results of serum physiology showed that the activity of superoxide dismutase in serum gradually increased with the increase in the percentage of SPP addition, and the F3 group produced a significant difference from the F0 group (p < 0.05). The transcriptomics results showed that DEGs in the low percentage substitution group (<15%) were mostly enriched in metabolism-related pathways, such as bile secretion; DEGs in the high percentage substitution group (>15%) were mostly enriched in inflammation-related pathways, such as complement p and coagulation cascades. Metabolomics confirmed that nicotinate and nicotinamide metabolism and glycerophospholipid metabolism were the two pathways that were significantly enriched in the treatment groups of fishmeal replacement by SPP. The present study demonstrated that a low percentage (<15%) of fishmeal replacement by SPP in feed mobilized MA digestive metabolism, whereas a high percentage (>15%) of replacement induced intestinal stress. Considering the health and farm efficiency aspects, the proportion of SPP in feed formulation for MA should be less than 15%. [ABSTRACT FROM AUTHOR]

The deteriorating soil health under continuous monoculture is commonly found across various cropping systems. This study evaluated the effects of different tillage practices (conventional tillage and no till) and species mixtures (legumes and grasses) on arbuscular mycorrhizal fungi (AMF) community properties, soil nutrients, and enzyme activity in a 3-year experiment. Compared with traditional tillage, the number of AMF species under no-till conditions was increased, with the Glomus group being dominant. Under different tillage conditions, TN (total N) and AN (available N) contents under no till were significantly higher than those under conventional tillage, while no significant differences among other nutrients were found. The activities of soil acid phosphatase (S-ACP), soil dehydrogenase (S-DHA), and soil sucrose (S-SC) under conventional tillage were significantly higher than those under no till, and the cover crop mixtures also had an exclusive advantage in yield. Soil organic matter (SOM) indicated a significant negative correlation with glomalin-related soil protein (GRSP). The increase in diversity associated with the AMF species community was strongly correlated with the increase in three enzyme activities, and AN was negatively correlated with all species. Tillage did not significantly change soil chemistry, except for AN, and the high concentration of AN led to a decrease in AMF species. The results of this study showed that no till was an effective measure for enriching soil micro-organism population. Additionally, soil AMF diversity was improved by cover crop mixtures, and microbial diversity was higher than that under monoculture cover crops. Different AMF groups responded differently to tillage and cover crop mixtures. Across all mixtures, the combination of hairy vetch (Vicia villosa R.) and ryegrass (Lolium perenne L.) performed the best. [ABSTRACT FROM AUTHOR]

The ongoing scientific debate on the selection of the best bioindicators to reflect the quality of arable soils indicates both their microbiome and biochemical parameters. Consideration has also been given to the fact that Zea mays has achieved the status of a crop used in the feed industry and for energy purposes, and Triticosecale is attracting increasing interest in this area. Therefore, the aim of this study was to determine the wide range of effects of Zea mays and Triticosecale cultivation on soil microbial and biochemical activity. The assessment of these parameters was based on the determination of microbial abundance, colony development index (CD), ecophysiological index of microbial diversity (EP), soil enzyme activities (dehydrogenases, catalase, urease, acid phosphatase, alkaline phosphatase, β-glucosidase, and arylsulfatase) as well as soil physicochemical properties. The innovative nature of the research was achieved by extending the pool of analyses to include both microbial biodiversity and analysis of soil samples at three depths: 0–20 cm; 21–40 cm; and 41–60 cm. It was found that the highest activities of soil enzymes and the abundance of organotrophic bacteria and fungi, as well as their colony development indices (CD), occurred within the rhizosphere and that their values decreased with increasing depth of the soil profile layers. Two phyla, Actinobacteria and Proteobacteria, representing the microbiome of arable soils, were identified independently of soil management practices. Unique bacterial genera in the soil under Triticosecale cultivation were Pseudonocardia, whereas Rhodoplanes, Nocardioides, and Rhodanobacter were found under Zea mays cultivation. The activity of all enzymes, especially urease and arylsulfatase, was significantly higher in the soil under Triticosecale. This was influenced by the more favorable physicochemical properties of the soil. [ABSTRACT FROM AUTHOR]

One of the most pressing global environmental issues is the widespread abundance and distribution of microplastics (MPs). MPs can act as vectors for other contaminants in the environment making these small plastic particles hazardous for ecosystems. The presence of MPs in aquatic environments may pose threats to aquatic organisms that ingest them. This study examined effects of abamectin (ABM) and polyethylene terephthalate (PET) MP fragments on histopathological and enzymatic biomarkers in zebrafish (Danio rerio). Zebrafish were exposed for 96 h to pristine PET-MPs at concentrations of 5 mg/L and 10 mg/L, ABM alone at 0.006 mg/L, and the same concentration of ABM in the presence of PET-MPs in aquaria. Histopathological analysis revealed tissue content changes in liver and kidney in the presence of ABM individually and in combination with MPs. Results of enzymatic analysis showed that MPs increased the bioavailability and toxicity of pesticides due to inhibition of catalase (CAT) and acid phosphatase (ACP) enzymes. However, MPs did not affect the toxicity of ABM for glutathione s-transferase (GST) enzyme. Despite the inhibition of acetylcholinesterase (AChE) in MPs or ABM treatments, and some neurotoxicity, no change in activity of this enzyme and neurotoxicity was observed in the combined MPs and ABM treatments, although toxicity effects of MPs and ABM on zebrafish require more detailed studies. [ABSTRACT FROM AUTHOR]

Anthropogenic inputs of nitrogen (N) and phosphorus (P) to terrestrial ecosystems alter soil nutrient cycling. However, the global‐scale responses of soil P fractions to N and P inputs and their underlying mechanisms remain elusive. We conducted a global meta‐analysis based on 818 observations of soil P fractions from 99 field N and P addition experiments in forest, grassland, and cropland ecosystems ranging from temperate to tropical zones. Our global meta‐analysis revealed distinct responses of soil P fractions to N and P enrichment. For studies using the Chang and Jackson inorganic (Pi) method, we found that high N addition promoted the transformation of immobile Pi fractions into Ferrum/Aluminum‐bound Pi and available Pi in surface soils through soil acidification. However, this acid‐induced transformation of Pi fractions by N addition was observed only in Calcium‐rich soils, while in acidic soils, further acidification led to increase P binding. In contrast, additions of P alone or combined with N significantly increased all soil Pi fractions. Regarding the Hedley P fractions, N addition generally decreased labile organic P by enhancing soil acid phosphatase activity. The responses of other P fractions were influenced by soil pH, fertilization rates, ecosystem type, and other factors. P addition increased most soil P fractions. Overall, both P fractionation methods consistently demonstrate that N inputs deplete soil P and accelerate P cycling, while P inputs increase most soil P fractions, alleviating P limitation. These findings are crucial for predicting the effects of future atmospheric N and P deposition on P cycling processes. Plain Language Summary: Human activities have increased the amount of nitrogen (N) and phosphorus (P) in the environment, which has led to changes in the soil nutrients cycle. This study examined the global‐scale responses of soil P fractions to N and P inputs using a data set from 99 field experiments worldwide. The findings revealed distinct responses of soil P fractions to N and P enrichment. High N input resulted in the transformation of immobile inorganic P (Pi) fractions into available Pi in surface soils. This transformation was observed in calcium‐rich soils due to soil acidification. In contrast, in acidic soils, the acidification led to increased Pi binding. Moreover, N input generally decreased labile organic P, potentially by enhancing soil enzyme activity. Addition of P alone or combined with N significantly increased soil P fractions. These findings have important implications for predicting the effects of future N and P deposition on P cycling processes in the terrestrial ecosystems and understanding the impacts of nutrient enrichment on soil carbon storage and eutrophication. Key Points: Nitrogen inputs can accelerate phosphorus (P) transformation in Calcium‐rich soils but promote P binding in acidic soilsSoil acidification reduces inorganic P bioavailability by increasing the P fixation of Ferrum and Alumiium oxidePhosphorus input increases soil labile, moderately labile, and occluded inorganic P, enhancing soil available P [ABSTRACT FROM AUTHOR]

Current and emerging legislation in North Carolina and other regions calls for the enumeration of Clostridium perfringens as a surrogate indicator for protozoan parasites in various types of waters. Past studies that have evaluated selective media for the detection of this bacterium have provided limited, conflicting, and inconclusive results. In this study, membrane filtration was used to enumerate C. perfringens as culturable spores or total culturable cells in 19 samples of untreated and 25 samples of partially treated wastewaters on 3 candidate media, Tryptose Sulfite Cycloserine Agar (TSC), CP ChromoSelect Agar (CPCS), and membrane Clostridium perfringens Agar (m-CP) in parallel, and the results were compared. Presumptive isolates from each agar were further subjected to phenotypic confirmation tests for acid phosphatase production and stormy fermentation to further determine the performance of each agar. The CPCS agar was determined to have the highest enumerative capacity of total C. perfringens cells when compared to both TSC agar and m-CP agar (p-value < 0.05), but there was no significant difference in its ability to detect spores when compared to TSC agar (p-value > 0.05). The overall specificity of CPCS agar as determined by agreement of results from both confirmation tests was 0.81, while the specificity of TSC agar was only 0.28. Based on its performance, ease of preparation and use and consistency of colony characteristics, CPCS agar is recommended as the preferred medium for C. perfringens enumeration in wastewater. [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate changes in the concentration of N-terminal type I collagen extension pro-peptide (PINP), tartrate-resistant acid phosphatase (TRAcP), and parathyroid hormone-related protein (PTHrP) in saliva during orthodontic treatment in order to evaluate whether changes in bone turnover marker (BTM) concentration can help highlight the effects of orthodontic mechanical loading in the absence of clinical evidence of tooth movement in terms of tooth movement. Saliva samples from 25 apparently healthy young subjects (10 females and 15 males) were collected using Salivette® (Sarstedt) with cotton swabs and the concentrations of PTHrP, TRAcP 5b, and PINP were analyzed at time 0 (T1), 25 days (T2), and at 45 days (T3). Differences in the median value of biomarker levels between baseline T1 and follow-up of the different groups (T2 and T3) were assessed using the non-parametric Mann–Whitney U test. Trough concentrations of P1NP, PTHrP, and TRAcP were 0.80 µg/L, 0.21 ng/mL, and 0.90 U/L above the method LOD. The non-parametric Mann–Whitney U test confirmed a statistically significant difference in T1 versus concentrations of T2 and T3. All subjects evaluated had a statistically significant difference between T1 vs. T3. when compared with the specific critical difference (RCV) for the analyte The results obtained demonstrate that the evaluation of BTM changes in saliva can help the evaluation of orthodontic procedures and the monitoring of biomechanical therapy. [ABSTRACT FROM AUTHOR]

Cockroaches, ubiquitous pests known for significant reproduction and as potential vectors of diseases, are notorious for their adoptability to a variety of insecticides that cause substantial challenges in pest control. The present research evaluated the LC50 concentrations of imidacloprid (0.65 mg/L) and clothianidin (2.5 μg/L) for Blattella germanica using topical and bait methods. The cockroaches were exposed to one‐third concentration of LC50 at a regular interval of 1, 2, 4, 8, 12, 16 and 20 days. During exposure, the activity of P450 monooxygenase increased, whereas the activity of brain acetylcholinesterase decreased. Lactate dehydrogenase, alkaline and acid phosphatase activity were highest on day 4, with a slight recovery on day 20. The elevated activities for superoxide dismutase and catalase, as well as increased lipid peroxidation levels on day 4, followed by a significant recovery in activity on day 20. The glutathione‐S‐transferase activity was elevated while the glutathione content was reduced, and a significant restoration was observed at the end of the experiment. The biochemical mechanisms of the organisms such as P450, esterase enzymes and oxidative systems, are actively involved in the detoxification mechanism. The study elucidated that the insecticide defence mechanism triggers detoxification pathways that encompass the metabolism of endogenous insecticidal compounds, with clothianidin showing greater efficacy and lower detoxification compared with imidacloprid. [ABSTRACT FROM AUTHOR]

Background: Gukang Capsule has been used as a complementary and alternative medicine (CAM) for the treatment of primary osteoporosis (POP) in China. The primary aim of this study was to assess the clinical effectiveness and safety of Gukang Capsule in POP patients. Methods: A systematic search was conducted across multiple academic databases including PubMed, Web of science, Cochrane Library, China National Knowledge Infrastructure, Chongqing VIP Information, and Wanfang database to identify randomized controlled trials investigating the Gukang Capsule in the treatment of POP. The screening process, data extraction, and assessment of methodological quality were conducted independently by two reviewers. Statistical analysis was performed using the Rev Man 5.3 software. Subgroup analysis was carried out through the combination of OPF. Subgroup analysis was performed according to whether OPF were combined. Stata 12.0 was used for sensitivity and bias analysis. Results: Nineteen studies were assessed that included 1804 participants. It was found that compared with the control group, the total effective rate (RR = 1.26, 95% CI, 1.20, 1.33), the Medical Outcomes Study Short-form 36 [RR = 1.26, 95% CI(1.20, 1.33)], the bone mineral density (BMD) of lumbar vertebra (SMD = 0.77, 95% CI, 0.48, 1.07), the BMD of femoral neck [SMD = 0.84, 95% CI(0.53, 1.14)], and the BMD of Ward's triangle (SMD = 0.64, 95% CI, 0.44, 0.85) of the Gukang Capsule experimental group were higher. Compared with the control group, the fracture healing time (SMD = -2.14, 95% CI, -2.45, -1.84), the bone specific alkaline phosphatase (BALP) levels in serum (SMD = -2.00, 95% CI, -2.83, -1.17), the tartrate resistant acid phosphatase 5b (TRACP-5b) levels in serum (SMD = -2.58, 95% CI, -3.87, -1.29) of the Gukang Capsule experimental group were lower. The bone glaprotein (BGP) levels in serum (SMD = -0.22, 95% CI, -1.86, 1.43) and the adverse events (RR = 0.80, 95% CI, 0.40, 1.63) of the experimental group and the control group have no difference. Conclusion: Gukang Capsule, as a CAM for the management of POP, exhibits the potential to enhance BMD and quality of life, expedite the healing time of OPF, diminish levels of BALP and TRACP-5b, and improve the total effective rate without increasing the adverse events. [ABSTRACT FROM AUTHOR]

AbstractGallic acid (GAL), rutin (RUT), and quercetin (QUE) are common antioxidant agents in fruits and vegetables with intriguing pharmacological effects. In the present study, we compared the therapeutic outcomes of GAL + QUE in comparison with GAL + RUT co-treatment in a busulfan (BUS) model of testicular injury in Wistar rats. BUS (4 mg kg−1 body weight (b.w) was injected intraperitoneally daily for 4 days. GAL + RUT or GAL + QUE (20 mg kg−1 b. w) was delivered by oral gavage for 52 days. Examination of the testes of BUS-treated rats both biochemically and under light microscopy revealed an increased level of lipid peroxidation, DNA fragmentation, glutathione-S-transferase, lactate dehydrogenase, gamma-glutamyl transpeptidase, alkaline phosphatase and acid phosphatase with a concomitant decrease in the level of antioxidants: glutathione, ascorbic acid, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities, suggesting testicular injury. Tissue sections confirmed the testicular injury-induced by BUS, including diminished spermatogenesis score index, tubular diameter, gonado-somatic index, testis weight, epithelia thickness and higher percentage of aberrant tubules. GAL + QUE co-administration had better recovery effects than GAL + RUT on the biochemical markers and protected against BUS-induced testicular damage. GAL + QUE treatment regimen has better capacity to maintain the antioxidant capacity of the testes and is more potent at reducing BUS-induced oxidative damage compared to GAL + RUT. [ABSTRACT FROM AUTHOR]

The rupture of the Fundão dam (Brazil) spread tailings contaminated with sodium and ether-amine into the Doce River Basin. Aiming at rehabilitating a contaminated riparian site, phytoremediation with native species of the Atlantic Forest was performed under four treatments: ES-1: physical remediation (sediment scraping) + chemical remediation (organic matter) + bioremediation (double inoculation with the arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis and the plant growth-promoting bacteria Bacillus subtilis); ES-2: chemical remediation + bioremediation; ES-3: physical remediation + chemical remediation; ES-4: chemical remediation. Ether-amine and sodium contents, plant growth and, soil quality parameters were compared among treatments and relative to preserved and degraded sites. Two years after planting, the outstanding plant growth was attributed to the phytoremediation of ether-amine and ammonium, followed by a significant increase in soil microbial biomass (Phospholipid fatty acids-PLFAs), particularly the Gram+ bacteria and total fungi but not AMF, whose response was independent of the inoculation. While sodium and ether-amine declined, soil K, P, NO3− contents, dehydrogenase and acid phosphatase activities, cation exchange capacity (CEC) and soil aggregation increased, especially in ES-1. Thus, such remediation procedures are recommended for the restoration of riparian areas affected by the Fundão tailings, ultimately improving sediment fertility, aggregation and stabilization. To the best of our knowledge, this is the only successful example of fragment of riparian Atlantic Forest successfully rehabilitated using native trees from the Atlantic Forest in the riparian zone affected by the contaminated Fundão dam tailings. For that, phytoremediation and bioremediation procedures were adopted to alleviate etheramine and sodium toxicity, resulting in the amelioration of soil fertility and particularly the stabilization of such highly disaggregated sediments, ultimately protecting the Doce river basin against these contaminants. [ABSTRACT FROM AUTHOR]

The current study investigated the therapeutic potential of quercetin and quercetin nanoparticles in alleviating liver toxicity induced by ethion in Wistar albino rats. Quercetin nanoparticles were synthesized using the ionic‐gelation method. Rats were exposed to ethion alone (at high and low doses) or in combination with quercetin or quercetin nanoparticles. Ethion exposure led to significant decreases in organ weight, along with elevated levels of glucose, triglycerides, total cholesterol, low‐density lipoprotein, and acid phosphatase, and reduced high‐density lipoprotein in serum. Hepatocellular biomarker enzymes were increased in the ethion‐exposed group, while antioxidant enzyme levels were decreased. Histopathological analysis revealed fatty changes, degeneration, and hemorrhages in the liver. Co‐administration of quercetin nanoparticles mitigated these effects, potentially by neutralizing reactive oxygen species generated by ethion. Molecular docking studies suggested a strong interaction between quercetin and Cyp2E1, implicating a role in detoxification. This study highlights the potential of nanoquercetin as a therapeutic supplement for pesticide intoxication management. [ABSTRACT FROM AUTHOR]

Dianthus barbatus linn. is widely used in gardens, mainly as flower beds and flower borders. The effects of different gradients of P on the growth and root morphology of Dianthus barbatus were studied to explore its morphological and physiological responses and adaptive strategies. Hence, this study provides a theoretical basis and practical guidance for D. barbatus production. Two soil substrates, namely loess and vegetable soil, and five phosphorus concentration gradients were set; no phosphorus application was used as the control. The morphology and physiology of D. barbatus were also investigated. Low-to-medium- and low-phosphorus treatments promoted the growth of D. barbatus in the above and underground parts of the plants grown on both substrates. Chlorophyll content, flower quantity, and acid phosphatase activity in the rhizosphere soil were significantly increased in the H1 and H2 treatments of loess and in the C4 treatment of vegetable soil. Thus, D. barbatus seems to reduce the damage caused by phosphorus stress by increasing chlorophyll content and root acid phosphatase activity. The latter was significantly higher in vegetable soil than in loess. Vegetable soil was more conducive to D. barbatus growth than loess. [ABSTRACT FROM AUTHOR]

Background: Myocardial infarction (MI) leads to enhanced activity of cardiac fibroblasts (CFs) and abnormal deposition of extracellular matrix proteins, resulting in cardiac fibrosis. Tartrate-resistant acid phosphatase 5 (ACP5) has been shown to promote cell proliferation and phenotypic transition. However, it remains unclear whether ACP5 is involved in the development of cardiac fibrosis after MI. The present study aimed to investigate the role of ACP5 in post-MI fibrosis and its potential underlying mechanisms. Methods: Clinical blood samples were collected to detect ACP5 concentration. Myocardial fibrosis was induced by ligation of the left anterior descending coronary artery. The ACP5 inhibitor, AubipyOMe, was administered by intraperitoneal injection. Cardiac function and morphological changes were observed on Day 28 after injury. Cardiac CFs from neonatal mice were extracted to elucidate the underlying mechanism in vitro. The expression of ACP5 was silenced by small interfering RNA (siRNA) and overexpressed by adeno-associated viruses to evaluate its effect on CF activation. Results: The expression of ACP5 was increased in patients with MI, mice with MI, and mice with Ang II-induced fibrosis in vitro. AubipyOMe inhibited cardiac fibrosis and improved cardiac function in mice after MI. ACP5 inhibition reduced cell proliferation, migration, and phenotypic changes in CFs in vitro, while adenovirus-mediated ACP5 overexpression had the opposite effect. Mechanistically, the classical profibrotic pathway of glycogen synthase kinase-3β (GSK3β)/β-catenin was changed with ACP5 modulation, which indicated that ACP5 had a positive regulatory effect. Furthermore, the inhibitory effect of ACP5 deficiency on the GSK3β/β-catenin pathway was counteracted by an ERK activator, which indicated that ACP5 regulated GSK3β activity through ERK-mediated phosphorylation, thereby affecting β-catenin degradation. Conclusion: ACP5 may influence the proliferation, migration, and phenotypic transition of CFs, leading to the development of myocardial fibrosis after MI through modulating the ERK/GSK3β/β-catenin signaling pathway. [ABSTRACT FROM AUTHOR]

Bacterial endophytes (120) were isolated from six halophytes (Distichlis spicata, Cynodon dactylon, Eragrostis obtusiflora, Suaeda torreyana, Kochia scoparia, and Baccharis salicifolia). These halophiles were molecularly identified and characterized with or without NaCl conditions. Characterization was based on tests such as indole acetic acid (IAA), exopolysaccharides (EPS), and siderophores (SID) production; solubilization of phosphate (P), potassium (K), zinc (Zn), and manganese (Mn); mineralization of phytate; enzymatic activity (acid and alkaline phosphatase, phytases, xylanases, and chitinases) and the mineralization/solubilization mechanisms involved (organic acids and sugars). Moreover, compatibility among bacteria was assessed. Eleven halophiles were characterized as highly tolerant to NaCl (2.5 M). The bacteria isolated were all different from each other. Two belonged to Bacillus velezensis and one to B. pumilus while the rest of bacteria were identified up to the genus level as belonging to Bacillus, Halobacillus, Halomonas, Pseudomonas, Nesterenkonia, and three strains of Oceanobacillus. The biochemical responses of nutrient solubilization and enzymatic activity were different between bacteria and were influenced by the presence of NaCl. Organic acids were involved in P mineralization and nutrient solubilization. Tartaric acid was common in the solubilization of P, Zn, and K. Maleic and vanillic acid were only detected in Zn and K solubilization, respectively. Furthermore, sugars appeared to be involved in the solubilization of nutrients; fructose was detected in the solubilization tests. Therefore, these biochemical bacterial characteristics should be corroborated in vivo and tested as a consortium to mitigate saline stress in glycophytes under a global climate change scheme that threatens to exacerbate soil salinity. [ABSTRACT FROM AUTHOR]

Femoral head necrosis (FHN) is a serious complication after femoral neck fractures (FNF), often linked to sclerosis around screw paths. Our study aimed to uncover the proteomic and metabolomic underpinnings of FHN and sclerosis using integrated proteomics and metabolomics analyses. We identified differentially expressed proteins (DEPs) and metabolites (DEMs) among three groups: patients with FNF (Group A), sclerosis (Group B), and FHN (Group C). Using the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment analyses, we examined the roles of these proteins and metabolites. Our findings highlight the significant differences across the groups, with 218 DEPs and 44 DEMs identified between the sclerosis and FNF groups, 247 DEPs and 31 DEMs between the FHN and sclerosis groups, and a stark 682 DEPs and 94 DEMs between the FHN and FNF groups. Activities related to carbonate dehydratase and hydrolase were similar in the FHN and sclerosis groups, whereas extracellular region and lysosome were prevalent in the FHN and FNF groups. Our study also emphasized the involvement of the PI3K-Akt pathway in sclerosis and FHN. Moreover, the key metabolic pathways were implicated in glycerophospholipid metabolism and retrograde endocannabinoid signaling. Using western blotting, we confirmed the pivotal role of specific genes/proteins such as ITGB5, TNXB, CA II, and CA III in sclerosis and acid phosphatase 5 and cathepsin K in FHN. This comprehensive analyses elucidates the molecular mechanisms behind sclerosis and FHN and suggests potential biomarkers and therapeutic targets, paving the way for improved treatment strategies. Further validation of the findings is necessary to strengthen the robustness and reliability of the results. [ABSTRACT FROM AUTHOR]

Triclosan (TCS), an antimicrobial additive in various personal and health care products, has been widely detected in aquatic environment around the world. The present study investigated the impacts of TCS in the gills of the fish, Cyprinus carpio employing histopathological, biochemical, molecular docking and simulation analysis. The 96 h LC50 value of TCS in C. carpio was found to be 0.968 mg/L. Fish were exposed to 1/1000th (1 µg/L), 1/100th (10 µg/L), and 1/10th (100 µg/L) of 96 h LC50 value for a period of 28 days. The histopathological alterations observed in the gills were hypertrophy, hyperplasia, edematous swellings, and fusion of secondary lamellae in TCS exposed groups. The severity of these alterations increased with both the concentration as well as the duration of exposure. The present study revealed that the activity of antioxidant enzymes such as superoxide dismutase, catalase, glutathione-S-transferase, glutathione reductase, glutathione peroxidase, and reduced glutathione content decreased significantly (p < 0.05) in both concentration and duration dependent manner. However, a significant (p < 0.05) increase in the activity of the metabolic enzymes such as acid phosphatase and alkaline phosphatase was observed in all three exposure concentrations of TCS from 7 to 28 days. The activity of acetylcholinesterase declined significantly (p < 0.05) from 7 to 28 days whereas the content of acetylcholine increased significantly at the end of 28 day. The experimental results were further confirmed by molecular docking and simulation analysis that showed strong binding of TCS with acetylcholinesterase enzyme. The study revealed that long-term exposure to sublethal concentrations of TCS can lead to severe physiological and histopathological alterations in the fish. [ABSTRACT FROM AUTHOR]

Macrophage colony‐stimulating factor (M‐CSF) and interleukin‐34 (IL‐34) are ligands for the colony‐stimulating factor‐1 receptor (CSF‐1r) expressed on the surface of monocyte/macrophage lineage cells. The importance of coordinated signaling between M‐CSF/receptor activator of the nuclear factor kappa‐Β ligand (RANKL) in physiological and pathological bone remodeling and alveolar bone loss in response to oral bacterial colonization is well established. However, our knowledge about the IL‐34/RANKL signaling in periodontal bone loss remains limited. Recently published cohort studies have demonstrated that the expression patterns of IL‐34 are dramatically elevated in gingival crevicular fluid collected from patients with periodontitis. Therefore, the present study aims to evaluate the effects of IL‐34 on osteoclastogenesis in vitro and in experimental ligature‐mediated model of periodontitis using male mice. Our initial in vitro study demonstrated increased RANKL‐induced osteoclastogenesis of IL‐34‐primed osteoclast precursors (OCPs) compared to M‐CSF‐primed OCPs. Using an experimental model of ligature‐mediated periodontitis, we further demonstrated elevated expression of IL‐34 in periodontal lesions. In contrast, M‐CSF levels were dramatically reduced in these periodontal lesions. Furthermore, local injections of mouse recombinant IL‐34 protein significantly elevated cathepsin K activity, increased the number of tartrate‐resistant acid phosphatase (TRAP)‐positive osteoclasts and promoted alveolar bone loss in periodontitis lesions. In contrast, anti‐IL‐34 neutralizing monoclonal antibody significantly reduced the level of alveolar bone loss and the number of TRAP‐positive osteoclasts in periodontitis lesions. No beneficial effects of locally injected anti‐M‐CSF neutralizing antibody were observed in periodontal lesions. This study illustrates the role of IL‐34 in promoting alveolar bone loss in periodontal lesions and proposes the potential of anti‐IL34 monoclonal antibody (mAb)‐based therapeutic regimens to suppress alveolar bone loss in periodontitis lesions. [ABSTRACT FROM AUTHOR]

The precise regulation of nanoenzyme activity is of great significance for application to biosensing analysis. Herein, the peroxidase-like activity of carbon dots was effectively modulated by doping phosphorus, which was successfully employed for sensitive, selective detection of acid phosphatase (ACP). Phosphorus-doped carbon dots (P-CDs) with excellent peroxidase-like activity were synthesized by a one-pot hydrothermal method, and the catalytic activity could be easily modulated by controlling the additional amount of precursor phytic acid. P-CDs could effectively catalyze the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue TMB oxidation products in the presence of hydrogen peroxide. While ACP was able to catalyze the hydrolysis of L-ascorbyl-2-phosphate trisodium salt (AAP) to produce ascorbic acid (AA), which inhibited the peroxidase-like activity of P-CDs, by combining P-CDs nanoenzymes and ACP-catalyzed hydrolysis the colorimetric method was established for ACP detection. The absorbance variation showed a good linear relationship with ACP concentration in the range of 0.4–4.0 mU/mL with a limit of detection at 0.12 mU/mL. In addition, the method was successfully applied to detect ACP in human serum samples with recoveries in the range of 98.7–101.6%. The work provides an effective strategy for regulating nanoenzymes activity and a low-cost detection technique for ACP. [ABSTRACT FROM AUTHOR]

Background and aims: Plants alter root placement patterns to respond nutrients and neighbors, but whether neighbors influence the root architecture by modifying the availability of nutrients is still unclear. Here we aimed to screen intercropping plants cause changes in tomato root architecture and explore the role and potential activation pathways of N and P nutrients. Methods: We carried out the experiments of tomato-neighbor interactions to survey root plastic responses of target tomato seedlings and root placement patterns to intercropping soybean under different P or N levels. In addition, we investigated the effects and physiological mechanisms of intercropping soybean on P capture of tomatoes. Results: The intercropping soybean changed the horizontal symmetry of tomato roots, the dry weight and total root length of tomato roots in the 'Toword' region were significantly higher than those in the 'Away' region, while other intercropping plants did not. P rather than N levels, significantly affected the tomato root placement patterns in soybean intercropping systems. Under phosphorus deficiency condition, tomato roots showed a tendency to soybean. This phenomenon disappeared when the P supply increased. Under low P condition, intercropping soybean significantly increased P concentration of tomato plants and rhizosphere soil available P content. Compared with tomatoes, soybean roots secreted more acid phosphatase, protons and organic acids. Conclusions: Soybean triggered tomato root horizontal asymmetry through modifying tomato P rather than N capture mediated by root exudates under low nutrient condition, which contributes to the selection and application of tomato cultivation models in low input agriculture. [ABSTRACT FROM AUTHOR]

Here, we hypothesized the occurrence of a compensatory relationship between the application of P and different microbial communities in the soil, specifically in relation to the chemical and biological effects in the soil–plant–microorganisms' interaction. We aimed to evaluate the plant–microbiota responses in plants grown in soils hosting distinct microbial communities and rates of P availability. Two experiments were carried out in a greenhouse. The first experiment evaluated four manipulated soil microbiome compositions, four P rates, and two plant species. Manipulated soil systems were obtained by the following: (i) autoclaving soil for 1 h at 121 °C (AS); (ii) inoculating AS with soil suspension dilution (AS + 10−3); (iii) heating natural soil at 80 °C for 1 h (NH80); or (iv) using natural soil (NS) without manipulation. The P rates added were 0, 20, 40, and 60 mg kg−1, and the two plant species tested were grass (brachiaria) and leguminous (crotalaria). Inorganic labile P (PAER), microbial P (PMIC), acid phosphatase activity (APASE), and shoot P uptake (PUPT) were assessed for each system. Brachiaria presented a compensatory effect for PUPT, whereby the addition of P under conditions of low microbial community enhanced P absorption capacity from the soil. However, in a system characterized by low P input, the increase in the soil biodiversity was insufficient to enhance brachiaria PUPT. Likewise, crotalaria showed a higher PUPT under high P application and low microbial community. The second experiment used three manipulated microbiome compositions: AS + 10−3; NH80; and NS and three P rates added: 0, 20, and 40 mg kg−1. In addition, two treatments were set: without and with mycorrhiza inoculation. Brachiaria showed an increase in the PUPT under low microbial communities (AS + 10−3; NH80) with P addition (20 and 40 mg kg−1 of P), but no mycorrhization was observed. In the undisturbed microbial community (NS), under no P input (0 mg kg−1 of P), brachiaria showed low mycorrhization and low PUPT. Finally, NS and the recommended P input (40 mg kg−1 of P) represented a balance between chemical and biological fertility, promoting the equilibrium between mycorrhization and PUPT. [ABSTRACT FROM AUTHOR]

Background: The Pati duck constitutes a major indigenous duck variety in the state of Assam. The study of the gut associated lymphoid tissue (GALT) of Pati duck of Assam is of great value in regard to normal academic and bio-medical research aspects. It is also pre-requisite for correct diagnosis and evaluating the treatment of certain diseases like duck virus enteritis, duck cholera, aflatoxicosis, botulism etc, caused by different types of pathogens, food poisoning and food allergy. Methods: The study was conducted on Pati duck of Assam in where 45 numbers of ducks were selected by irrespective of sex at different stages of development. The birds were divided into five groups depending on its age viz., 1st week, 4th week, 16th week, 24th week and 42nd weeks old. The pieces of gut having lymphoid tissue or Peyer's patches were collected immediately after slaughter. Samples were made cryosections (-20.C) at 10 µm in thickness and were temporally stored at (-22.C). The histochemical staining was done after that. Result: The acid phosphatase activity was weak in the lymphoid follicles of 1st and 4th week of age of Pati duck, while its activity was moderate in 16th, 24th and 42th week of age. The alkaline phosphatase activity was moderate reaction in the lymphoid follicles of 1st and 4th week of age of Pati duck, while its activity was intense in 16th, 24th and 42th week of age of duck. The lymphoid follicles of both Peyer's patches and solitary lymphoid nodules were showed moderate activity for adenosine tri-phosphatase activity in 1st and 4th week old Pati duck and strong activity in 16th, 24th and 42th week of age of Pati duck, respectively. The lymphoid nodules of intestine showed strong reaction for non-specific esterase activity in all the age groups of Pati duck. [ABSTRACT FROM AUTHOR]

We performed a comprehensive study of protein (total protein, medium-molecular-weight peptides, creatinine, and urea), purine (uric acid), and lipid (cholesterol, triglycerides) metabolism, activity of AST, ALT, and acid phosphatase in blood plasma of white male rats under conditions of restriction of motor activity up to 28 days. Patterns of changes in metabolic profile during hypokinesia were established: prevalence of catabolic processes and atherogenic shifts in the lipid spectrum with maximum manifestation on 14-21 days of the experiment. [ABSTRACT FROM AUTHOR]

A detailed effect of continuous exposure of Cd on an important pest of crops like Helicoverpa armigera is lacking in the current literature. The cadmium was provisioned at 1.25, 2.5 and 5 mg/kg in the artificial diet and fed to newly hatched larvae for at least three subsequent generations. The total developmental period and fecundity were severely affected with no larval hatching in treated insects. Total haemocyte count and differential haemocyte count (i.e., granulocytes and plasmatocytes) were decreased significantly compared to controls in treated insects. The antioxidant system (i.e., catalase, glucose‐6‐phosphate dehydrogenase, superoxide dismutase and peroxidase), and the two detoxifying agents glutathione‐s‐transferase showed increased activity compared to controls. The results of the acetylcholinesterase assay showed a decrease in activity. Increased rate of aspartate aminotransferase, alanine aminotransferase, uric acid and decreased rate of alkaline phosphatase and lactate dehydrogenase in the first generation but showing an increasing trend in next two generations. The acid phosphatase activity was increased in the first two generations but significantly reduced in the third generation. The discrepancy in all physiological activities corresponded with the histological study of midgut infrastructure. It is inferred from the obtained results that cadmium continuous exposure affects every system of any organism that may perform irreparable damages. [ABSTRACT FROM AUTHOR]

A histidine acid phosphatase (HAP) (PhySc) with 99.50% protein sequence similarity with PHO5 from Saccharomyces cerevisiae was expressed functionally with the molecular mass of ∼110 kDa through co-expression along with the set of molecular chaperones dnaK, dnaJ, GroESL. The purified HAP illustrated the optimum activity of 28.75 ± 0.39 U/mg at pH 5.5 and 40 ˚C. The Km and Kcat values towards calcium phytate were 0.608 ± 0.09 mM and 650.89 ± 3.6 s− 1. The half-lives (T1/2) at 55 and 60 ˚C were 2.75 min and 55 s, respectively. The circular dichroism (CD) demonstrated that PhySc includes 30.5, 28.1, 21.3, and 20.1% of random coils, α-Helix, β-Turns, and β-Sheet, respectively. The Tm recorded by CD for PhySc was 56.5 ± 0.34˚C. The molecular docking illustrated that His59 and Asp322 act as catalytic residues in the PhySc. MD simulation showed that PhySc at 40 ˚C has higher structural stability over those of the temperatures 60 and 80 ˚C that support the thermodynamic in vitro investigations. Secondary structure content results obtained from MD simulation indicated that PhySc consists of 34.03, 33.09, 17.5, 12.31, and 3.05% of coil, helix, turn, sheet, and helix310, respectively, which is almost consistent with the experimental results. [ABSTRACT FROM AUTHOR]

This study investigated the effects of acute ammonia nitrogen (NH3-N) exposure on kidney antioxidant ability and phosphatases and related gene expression in juvenile yellowfin tuna (Thunnus albacares). The 180 juvenile yellowfin tuna (260.39 ± 55.99 g, 22.33 ± 2.28 cm) were exposed to ammonia for 6, 24, and 36 h using natural seawater (0 mg/L) as a control and NH3-N at 5 and 10 mg/L. The lipid peroxidation byproduct malondialdehyde (MDA) and the levels of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX), alkaline phosphatase (AKP), and acid phosphatase (ACP), were measured using the colorimetric method in the trunk kidney to determine changes in antioxidant ability and phosphatase activity of juvenile yellowfin tuna exposed to NH3-N. Results indicated that, at 36 h, MDA, SOD, CAT, and GSH-PX levels rose in the 5 mg/L group versus the control. In the 10 mg/L group, MDA and SOD, CAT, and GSH-PX activities significantly increased after 24 and 36 h exposure compared to the control. Phosphatases play a pivotal role in the immune system. AKP activity significantly increased at 6 h, and ACP activity markedly rose at 36 h in the 5 mg/L group versus the control. Real-time fluorescence quantitative PCR was applied to detect alterations in the antioxidant genes SOD2, CAT, and glutathione peroxidase 1b (GPX1b) and immune cytokines-related genes Interleukin 10 (IL-10) and Interleukin 6 receptor (IL-6r) expression in the head kidney in juvenile tuna. Relative to the control, antioxidant gene expression in the 5 mg/L group significantly rose at 6 and 36 h, and in the 10 mg/L group, SOD2 and GPX1b were significantly elevated at 36 h. Compared to the control group, IL-10 expression in the 5 mg/L group significantly increased at 6 h, whereas IL-6r expression decreased. In the 10 mg/L group, both IL-10 and IL-6r levels were observed to be lower. Low ammonia nitrogen concentrations boost antioxidant defenses, phosphatase activities, and gene expression levels, whereas higher levels may induce suppressive effects. In yellowfin tuna juvenile farming, NH3-N concentration significantly affects the health of the juveniles. When the NH3-N concentration is between 5–10 mg/L, the stress duration should be limited to 24 h; if the concentration is below 5 mg/L, the stress duration can be extended to 36 h. [ABSTRACT FROM AUTHOR]

Bamboo and wood-mixed forests are management models that remarkably enhance the balance and productivity of bamboo ecosystems. However, the effects of this model on soil nutrients and enzyme activities remain largely unknown. This study compared the soil organic carbon, nitrogen, phosphorus, and enzyme activity, along with the characteristics of fine roots in pure Moso bamboo plantations (CK) and those mixed with Liriodendron chinense (ML), Sassafras tzumu (MS), Cunninghamia lanceolata (MC), and Pseudolarix amabilis (MP). The results showed that mixed forests improve carbon pools in 0–40 cm soil layers, increasing the total organic C(TOC), free particulate organic C (fPOC), occluded particulate organic C (oPOC), hot-water-extractable organic C (DOC), and mineral-associated organic C (MOC). They also increase soil total N, total P, available N, available P, NH4+-N, NO3−-N, inorganic P, organic P, and microbial biomass N. Bacterial and fungal abundances, along with enzyme activities (urease, acid phosphatase, polyphenol oxidase, peroxidase, and β-glucosidase), also improved. MP and MS were the most effective. Moreover, MS and MP supported a higher biomass and length of fine root and increased the nitrogen and phosphorus uptake of Moso bamboo. In conclusion, Sassafras tzumu and Pseudolarix amabilis are optimal for mixed planting, offering substantial benefits to soil nutrient dynamics and preventing soil quality decline in Moso bamboo forests, thereby supporting better nutrient cycling and carbon sequestration. This research offers insights into enhancing soil quality through diversified Moso bamboo forestry. [ABSTRACT FROM AUTHOR]

Perfluorooctanoic acid (PFOA) exposure level in human is negatively correlated with bone density according to the epidemiological research. Our study is aim to investigate the effects and mechanisms of PFOA on osteoblastic bone formation and osteoclastic bone resorption. Primary osteoblasts were isolated from the calvaria of newborn rats, and the cell proliferation, alkaline phosphatase (ALP) activity, and bone nodule formation were detected after 1,10, and 100 μmol/L PFOA treateatment for 24 h.The osteoclasts model were differentiation from bone marrow monocyte, which induced by Macrophage colony-stimulating factor (M-CSF) and Nuclear factor κB receptor-activating factor ligand (RANKL), and the effects on cell viability, anti-tartaric acid phosphatase (TRAP) activity, and bone resorption pit formation were investigated. The expression of OPG and RANKL proteins in osteoblasts were conducted by Western blot. The results show that PFOA at the concentration of 1 μmol/L and 10 μmol/L can promote osteoblast proliferation, but has no effect on ALP activity and bone nodule formation. 100 μmol/L PFOA can significantly inhibit cell proliferation, ALP activity, and bone nodule formation, as well as the proportion of OPG/RANKL protein expression. PFOA has no effect on osteoclast viability, and 10 μmol/L and 100 μmol/L PFOA can significantly inhibit the TRAP activity and the area of bone resorption pits. The conclusion can be draw that high concentration of PFOA can inhibit the bone formation of osteoblasts by suppressing WNT pathway and promote osteoclast bone resorption by inhibiting the OPG/RANKL/RANK pathway. [ABSTRACT FROM AUTHOR]

Purpose: The invasive plant Flaveria bidentis (L.) Kuntze, known as the "ecological killer", poses a significant threat to the ecosystem stability due to its strong phenotypic plasticity and high fertility. Previous studies have reported a close relationship between plant invasion and soil microbial communities. This study aimed to gain a deeper understanding of the relationship between the invasion of F. bidentis, the diversity of soil microbial communities, and soil physiochemical properties. Methods: In this study, we established a common garden experiment by planting F. bidentis and Chenopodium ficifolium Sm. separately and mixed, in Lanfang, China. We analyzed microbial communities in both bulk soil and rhizosphere soil using the high-throughput sequencing technology. Results: The results showed that F. bidentis invasion significantly increased bacterial and fungal diversities in rhizosphere soil, irrelevant with or without the existence of native plant species. Meanwhile, F. bidentis increased the content of organic matter and ammonium nitrogen (NH4+) in bulk soil and decrease the content of NH4+ in rhizosphere soil. Significant correlations were observed between nitrogen contents or acid phosphatase and specific soil microbial communities. Conclusions: These results underly that regardless of competition with native plants, the invasion of F. bidentis alters soil physicochemical properties and increases microorganism community diversity. This study establishes a theoretical foundation for further research on the invasion mechanisms of F. bidentis and provides valuable references for other studies about F. bidentis. [ABSTRACT FROM AUTHOR]