348 results on '"Abramo F"'
Search Results
102. High-Resolution Ultrasonography of Experimentally Induced Full-Thickness Canine Skin Wounds: Efficacy in Imaging Canine Skin and Comparison of 2 Methods of Measuring Wound Size
- Author
-
Mantis, P., Lloyd, D. H., Dirk Pfeiffer, Stevens, K., Auxilia, S., Noli, C., and Abramo, F.
103. Pathology of naturally acquired Angiostrongylus vasorum infection in the red fox (Vulpes vulpes)
- Author
-
Alessandro Poli, Arispici, M., Mancianti, F., and Abramo, F.
104. A method for identification and characterization of ewe's milk fat globules
- Author
-
Scolozzi, C., Mina Martini, and Abramo, F.
105. Siderosi sperimentale nel coniglio. Valutazioni preliminari sugli effetti tossici a carico della retina e delle strutture angolari
- Author
-
Trombetta, C. J., Nastasi, F., Abramo, F., Trombetta, C. J., Nastasi, F., and Abramo, F.
- Abstract
Gli autori riferiscono i risultati preliminari di uno studio sperimentale su conigli sottoposti alla introduzione di un corpo estraneo endobulbare in ferro, allo scopo di valutare la reattività delle strutture cellulari fagocitanti dell'occhio normale: epitelio pigmentato della retina ed endotelio del trabecolato.
106. Siderosi sperimentale nel coniglio. Valutazioni preliminari sugli effetti tossici a carico della retina e delle strutture angolari
- Author
-
Trombetta, C. J., Nastasi, F., Abramo, F., Trombetta, C. J., Nastasi, F., and Abramo, F.
- Abstract
Gli autori riferiscono i risultati preliminari di uno studio sperimentale su conigli sottoposti alla introduzione di un corpo estraneo endobulbare in ferro, allo scopo di valutare la reattività delle strutture cellulari fagocitanti dell'occhio normale: epitelio pigmentato della retina ed endotelio del trabecolato.
107. Die perforierende Keratoplastik - Indikationen unter besonderer Berücksichtigung der Kunstlinsenkeratopathie
- Author
-
Burk, R., primary, Böhnke, M., additional, Domarus, D. von, additional, and Abramo, F., additional
- Published
- 1988
- Full Text
- View/download PDF
108. Die okuläre Myositis - Morphologie und Funktion - Langzeitbeobachtungen bei 15 Patienten
- Author
-
Guthoff, R., primary, Abramo, F., additional, and Jørgensen, J., additional
- Published
- 1988
- Full Text
- View/download PDF
109. A randomized, double-blinded, placebo- controlled multicenter study on the efficacy of a diet with high levels of eicosapentaenoic acid and gamma-linolenic acid in the control of canine atopic dermatitis.
- Author
-
Abramo, F., Mantis, P., Lloyd, D. H., Auxilia, S. T., Noli, C., Leotta, R., and Pfeiffer, D.
- Subjects
- *
ATOPIC dermatitis , *ALLERGIES , *ITCHING , *ERYTHEMA , *FATTY acids , *ADRENOCORTICAL hormones , *ANTI-infective agents , *INFECTION - Abstract
The purpose of this study was to evaluate the efficacy of a diet with high levels of eicosapentaenoic acid (EPA) (480 mg/megajoule) and gamma-linolenic acid (GLA) (50 mg/megajoule) in the control of clinical signs in dogs with atopic dermatitis. Selection of dogs with atopic dermatitis occurred according to a strict protocol; dogs with ectoparasites, endoparasites, dermatophytosis, Malassezia infection, active flea allergy dermatitis, hypothyroidism or food allergy were excluded, as well as dogs currently treated with hyposensitization, glucocorticoids, antihistamines, NSAIDs and dietary fatty acid supplements. Dogs were only selected if they met the diagnostic criteria for atopy and when atopy was confirmed by a positive intradermal test correlating with the history and clinical signs. During the entire study, flea control was applied and secondary pyoderma or Malassezia infections were controlled with antibiotics or topically applied lotions. The owners were informed that the diet and/or capsules might be treatment or placebo. The dogs received either a treatment with the test diet plus a daily placebo capsule, or the dog's usual diet plus a daily placebo capsule for a period of 10 weeks. The treatment diet provided 240 mg EPA/kg metabolic weight and 25 mg GLA/kg metabolic weight. Owners who judged that the treatment during the study had not been effective could participate in a double-blinded crossover period during which the dog received another treatment. At the start and end of the study, the severity of the following clinical signs was scored: pruritus, shedding, scaling, dry skin, erythema, smell of coat and alopecia. In total, 13 dogs received the control treatment and 15 dogs the test diet. Of the 13 control dogs, nine followed a crossover period on the test diet, which brought the total number of dogs on the diet to 24. The score after 10 weeks of treatment was expressed as percentage of the score at week 0. After 10 weeks of treatment, scores for intensity, frequency and total score for pruritus in dogs fed the test diet were significantly lower than scores for the control dogs ( P = 0.005, P = 0.013, P = 0.005, respectively); the average score was reduced to 67, 65 and 53% of the initial score, respectively. For dogs on the diet, the score for erythema was significantly lower than the score from the control dogs ( P = 0.019) and was reduced to 66% of the initial score. Thirteen of the 24 owners of the dogs fed the test diet (54%) indicated that the skin and coat condition of their dog had improved compared to four of 13 control dogs (31%, P = 0.030). The dogs’ plasma and cutaneous fatty acid compositions changed significantly on the test diet and were similar for responders and nonresponders. From this study, it can be concluded that a diet with high levels of EPA and GLA is effective in the reduction of pruritus and erythema in dogs with atopic dermatitis, but that not all dogs respond to the diet. Funding: LEO Animal Health. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
110. Comparative expression pathway analysis of human and canine mammary tumors
- Author
-
Marconato Laura, Zappulli Valentina, Mesiti Giuseppe, Viti Valentina, Palombo Fabio, Castle John, Kulkarni Amit, Loboda Andrey, Watters James, Aurisicchio Luigi, Uva Paolo, Abramo Francesca, Ciliberto Gennaro, Lahm Armin, La Monica Nicola, and de Rinaldis Emanuele
- Subjects
Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Spontaneous tumors in dog have been demonstrated to share many features with their human counterparts, including relevant molecular targets, histological appearance, genetics, biological behavior and response to conventional treatments. Mammary tumors in dog therefore provide an attractive alternative to more classical mouse models, such as transgenics or xenografts, where the tumour is artificially induced. To assess the extent to which dog tumors represent clinically significant human phenotypes, we performed the first genome-wide comparative analysis of transcriptional changes occurring in mammary tumors of the two species, with particular focus on the molecular pathways involved. Results We analyzed human and dog gene expression data derived from both tumor and normal mammary samples. By analyzing the expression levels of about ten thousand dog/human orthologous genes we observed a significant overlap of genes deregulated in the mammary tumor samples, as compared to their normal counterparts. Pathway analysis of gene expression data revealed a great degree of similarity in the perturbation of many cancer-related pathways, including the 'PI3K/AKT', 'KRAS', 'PTEN', 'WNT-beta catenin' and 'MAPK cascade'. Moreover, we show that the transcriptional relationships between different gene signatures observed in human breast cancer are largely maintained in the canine model, suggesting a close interspecies similarity in the network of cancer signalling circuitries. Conclusion Our data confirm and further strengthen the value of the canine mammary cancer model and open up new perspectives for the evaluation of novel cancer therapeutics and the development of prognostic and diagnostic biomarkers to be used in clinical studies.
- Published
- 2009
- Full Text
- View/download PDF
111. Different media and supplements modulate the clonogenic and expansion properties of rabbit bone marrow mesenchymal stem cells
- Author
-
Vanacore Renato, Stornelli Maria, Giannessi Elisabetta, Miragliotta Vincenzo, Abramo Francesca, Coli Alessandra, Urciuoli Patrizia, Paolicchi Aldo, Iorio Mariacarla, Passeri Simona, Lamanna Roberta, Nocchi Francesca, Lapi Simone, Stampacchia Giulia, Pisani Guido, Borghetti Luciano, and Scatena Fabrizio
- Subjects
Medicine ,Biology (General) ,QH301-705.5 ,Science (General) ,Q1-390 - Abstract
Abstract Background - Rabbits provide an excellent model for many animal and human diseases, such as cardiovascular diseases, for the development of new vaccines in wound healing management and in the field of tissue engineering of tendon, cartilage, bone and skin. The study presented herein aims to investigate the biological properties of bone marrow rabbit MSCs cultured in different conditions, in order to provide a basis for their clinical applications in veterinary medicine. Findings - MSCs were isolated from 5 New Zealand rabbits. Fold increase, CFU number, doubling time, differentiation ability and immunophenotype were analyzed. With the plating density of 10 cells/cm2 the fold increase was significantly lower with DMEM-20%FCS and MSCs growth was significantly higher with αMEM-hEGF. The highest clonogenic ability was found at 100 cell/cm2 with MSCBM and at 10 cell/cm2 with M199. Both at 10 and 100 cells/cm2, in αMEM medium, the highest CFU increase was obtained by adding bFGF. Supplementing culture media with 10%FCS-10%HS determined a significant increase of CFU. Conclusion - Our data suggest that different progenitor cells with differential sensitivity to media, sera and growth factors exist and the choice of culture conditions has to be carefully considered for MSC management.
- Published
- 2008
- Full Text
- View/download PDF
112. Renal involvement in mice experimentally infected with Toxocara canis embryonated eggs
- Author
-
Casarosa, L., Papini, R., Mancianti, F., Abramo, F., and Poli, A.
- Published
- 1992
- Full Text
- View/download PDF
113. Clinical features and comorbidity pattern of HCV infected migrants compared to native patients in care in Italy: A real-life evaluation of the PITER cohort
- Author
-
Maria Giovanna Quaranta, Luigina Ferrigno, Xhimi Tata, Franca D'Angelo, Marco Massari, Carmine Coppola, Elisa Biliotti, Alessia Giorgini, Diletta Laccabue, Alessia Ciancio, Pier Luigi Blanc, Marzia Margotti, Donatella Ieluzzi, Maurizia Rossana Brunetto, Francesco Barbaro, Francesco Paolo Russo, Ilaria Beretta, Giulia Morsica, Gabriella Verucchi, Annalisa Saracino, Massimo Galli, Loeta A. Kondili, Cesare Mazzaro, Manuela Bertola, Ornella Schioppa, Antonio Benedetti, Laura Schiadà, Monica Cucco, Andrea Giacometti, Laura Brescini, Sefora Castelletti, Alessandro Fiorentini, Gioacchino Angarano, Michele Milella, Alfredo Di Leo, Maria Rendina, Fulvio Salvatore D'abramo, Chiara Lillo, Andrea Iannone, Mariano Piazzolla, Lorenzo Badia, Fabio Piscaglia, Francesca Benevento, Ilaria Serio, Francesco Castelli, Serena Zaltron, Angiola Spinetti, Silvia Odolini, Raffaele Bruno, Mario Mondelli, Luchino Chessa, Martina Loi, Carlo Torti, Chiara Costa, Maria Mazzitelli, Vincenzo Pisani, Vincenzo Scaglione, Enrico Maria Trecarichi, Anna Linda Zignego, Monica Monti, Francesco Madia, Letizia Attala, Piera Pierotti, Elena Salomoni, Elisa Mariabelli, Teresa Antonia Santantonio, Serena Rita Bruno, Ester Marina Cela, Matteo Bassetti, Giovanni Mazzarello, Anna Ida Alessandrini, Antonio Di Biagio, Laura Ambra Nicolini, Giovanni Raimondo, Roberto Filomia, Alessio Aghemo, Rossella Meli, Adriano Lazzarin, Stefania Salpietro, Anna Ludovica Fracanzani, Erika Fatta, Rosa Lombardi, Pietro Lampertico, Marta Borghi, Roberta D'ambrosio, Elisabetta Degasperi, Massimo Puoti, Chiara Baiguera, Federico D'amico, Maria Vinci, Maria Grazia Rumi, Massimo Zuin, Paola Zermiani, Pietro Andreone, Paolo Caraceni, Valeria Guarneri, Erica Villa, Veronica Bernabucci, Laura Bristot, Maria Luisa Paradiso, Guglielmo Migliorino, Alessandra Gambaro, Giuseppe Lapadula, Anna Spolti, Alessandro Soria, Pietro Invernizzi, Antonio Ciaccio, Martina LucÀ, Federica Malinverno, Laura Ratti, Daniela Caterina Amoruso, Federica Pisano, Ferdinando Scarano, Laura Staiano, Filomena Morisco, Valentina Cossiga, Ivan Gentile, Antonio Riccardo Buonomo, Maria Foggia, Emanuela Zappulo, Alessandro Federico, Marcello Dallio, Nicola Coppola, Caterina Sagnelli, Salvatore Martini, Caterina Monari, Gerardo Nardone, Costantino Sgamato, Liliana Chemello, Luisa Cavalletto, Daniela Sterrantino, Alberto Zanetto, Paola Zanaga, Giuseppina Brancaccio, Antonio Craxì, Salvatore Petta, Vincenza Calvaruso, Luciano Crapanzano, Salvatore Madonia, Marco Cannizzaro, Erica Maria Bruno, Anna Licata, Simona Amodeo, Adele Rosaria Capitano, Carlo Ferrari, Elisa Negri, Alessandra Orlandini, Marco Pesci, Roberto Gulminetti, Layla Pagnucco, Giustino Parruti, Paola Di Stefano, Barbara Coco, Romina Corsini, Elisa Garlassi, Massimo Andreoni, Elisabetta Teti, Carlotta Cerva, Lorenzo Baiocchi, Giuseppe Grassi, Antonio Gasbarrini, Maurizio Pompili, Martina De Siena, Gloria Taliani, Martina Spaziante, Marcello Persico, Mario Masarone, Andrea Aglitti, Gemma Calvanese, Marco Anselmo, Pasqualina De Leo, Monica Marturano, Giorgio Maria Saracco, Quaranta M.G., Ferrigno L., Tata X., D'Angelo F., Massari M., Coppola C., Biliotti E., Giorgini A., Laccabue D., Ciancio A., Blanc P.L., Margotti M., Ieluzzi D., Brunetto M.R., Barbaro F., Russo F.P., Beretta I., Morsica G., Verucchi G., Saracino A., Galli M., Kondili L.A., Mazzaro C., Bertola M., Benedetti A., Schiada L., Cucco M., Giacometti A., Brescini L., Castelletti S., Fiorentini A., Angarano G., Milella M., Leo A.D., Rendina M., Salvatore D'ABRAMO F., Lillo C., Iannone A., Piazzolla M., Badia L., Piscaglia F., Benevento F., Serio I., Castelli F., Zaltron S., Spinetti A., Odolini S., Bruno R., Mondelli M., Chessa L., Loi M., Torti C., Costa C., Mazzitelli M., Pisani V., Scaglione V., Trecarichi E.M., Zignego A.L., Monti M., Madia F., Attala L., Pierotti P., Salomoni E., Mariabelli E., Santantonio T.A., Bruno S.R., Cela E.M., Bassetti M., Mazzarello G., Alessandrini A.I., Biagio A.D., Nicolini L.A., Raimondo G., Filomia R., Aghemo A., Meli R., Lazzarin A., Salpietro S., Fracanzani A.L., Fatta E., Lombardi R., Lampertico P., Borghi M., D'ambrosio R., Degasperi E., Puoti M., Baiguera C., D'AMICO F., Vinci M., Rumi M.G., Zuin M., Zermiani P., Andreone P., Caraceni P., Guarneri V., Villa E., Bernabucci V., Bristot L., Paradiso M.L., Migliorino G., Gambaro A., Lapadula G., Spolti A., Soria A., Invernizzi P., Ciaccio A., LucA M., Malinverno F., Ratti L., Amoruso D.C., Pisano F., Scarano F., Staiano L., Morisco F., Cossiga V., Gentile I., Buonomo A.R., Foggia M., Zappulo E., Federico A., Dallio M., Coppola N., Sagnelli C., Martini S., Monari C., Nardone G., Sgamato C., Chemello L., Cavalletto L., Sterrantino D., Zanetto A., Zanaga P., Brancaccio G., Craxi A., Petta S., Calvaruso V., Crapanzano L., Madonia S., Cannizzaro M., Bruno E.M., Licata A., Amodeo S., Capitano A.R., Ferrari C., Negri E., Orlandini A., Pesci M., Gulminetti R., Pagnucco L., Parruti G., Stefano P.D., Coco B., Corsini R., Garlassi E., Andreoni M., Teti E., Cerva C., Baiocchi L., Grassi G., Gasbarrini A., Pompili M., Siena M.D., Taliani G., Spaziante M., Persico M., Masarone M., Aglitti A., Calvanese G., Anselmo M., Leo P.D., Marturano M., Saracco G.M., Quaranta, M, Ferrigno, L, Tata, X, D'Angelo, F, Massari, M, Coppola, C, Biliotti, E, Giorgini, A, Laccabue, D, Ciancio, A, Blanc, P, Margotti, M, Ieluzzi, D, Brunetto, M, Barbaro, F, Russo, F, Beretta, I, Morsica, G, Verucchi, G, Saracino, A, Galli, M, Kondili, L, Mazzaro, C, Bertola, M, Benedetti, A, Schiada, L, Cucco, M, Giacometti, A, Brescini, L, Castelletti, S, Fiorentini, A, Angarano, G, Milella, M, Leo, A, Rendina, M, Salvatore D'ABRAMO, F, Lillo, C, Iannone, A, Piazzolla, M, Badia, L, Piscaglia, F, Benevento, F, Serio, I, Castelli, F, Zaltron, S, Spinetti, A, Odolini, S, Bruno, R, Mondelli, M, Chessa, L, Loi, M, Torti, C, Costa, C, Mazzitelli, M, Pisani, V, Scaglione, V, Trecarichi, E, Zignego, A, Monti, M, Madia, F, Attala, L, Pierotti, P, Salomoni, E, Mariabelli, E, Santantonio, T, Bruno, S, Cela, E, Bassetti, M, Mazzarello, G, Alessandrini, A, Biagio, A, Nicolini, L, Raimondo, G, Filomia, R, Aghemo, A, Meli, R, Lazzarin, A, Salpietro, S, Fracanzani, A, Fatta, E, Lombardi, R, Lampertico, P, Borghi, M, D'Ambrosio, R, Degasperi, E, Puoti, M, Baiguera, C, D'Amico, F, Vinci, M, Rumi, M, Zuin, M, Zermiani, P, Andreone, P, Caraceni, P, Guarneri, V, Villa, E, Bernabucci, V, Bristot, L, Paradiso, M, Migliorino, G, Gambaro, A, Lapadula, G, Spolti, A, Soria, A, Invernizzi, P, Ciaccio, A, Luca, M, Malinverno, F, Ratti, L, Amoruso, D, Pisano, F, Scarano, F, Staiano, L, Morisco, F, Cossiga, V, Gentile, I, Buonomo, A, Foggia, M, Zappulo, E, Federico, A, Dallio, M, Coppola, N, Sagnelli, C, Martini, S, Monari, C, Nardone, G, Sgamato, C, Chemello, L, Cavalletto, L, Sterrantino, D, Zanetto, A, Zanaga, P, Brancaccio, G, Craxi, A, Petta, S, Calvaruso, V, Crapanzano, L, Madonia, S, Cannizzaro, M, Bruno, E, Licata, A, Amodeo, S, Capitano, A, Ferrari, C, Negri, E, Orlandini, A, Pesci, M, Gulminetti, R, Pagnucco, L, Parruti, G, Stefano, P, Coco, B, Corsini, R, Garlassi, E, Andreoni, M, Teti, E, Cerva, C, Baiocchi, L, Grassi, G, Gasbarrini, A, Pompili, M, Siena, M, Taliani, G, Spaziante, M, Persico, M, Masarone, M, Aglitti, A, Calvanese, G, Anselmo, M, Leo, P, Marturano, M, Saracco, G, Quaranta, M. G., Ferrigno, L., Tata, X., D'Angelo, F., Massari, M., Coppola, C., Biliotti, E., Giorgini, A., Laccabue, D., Ciancio, A., Blanc, P. L., Margotti, M., Ieluzzi, D., Brunetto, M. R., Barbaro, F., Russo, F. P., Beretta, I., Morsica, G., Verucchi, G., Saracino, A., Galli, M., Kondili, L. A., Mazzaro, C., Bertola, M., Benedetti, A., Schiada, L., Cucco, M., Giacometti, A., Brescini, L., Castelletti, S., Fiorentini, A., Angarano, G., Milella, M., Leo, A. D., Rendina, M., Salvatore D'ABRAMO, F., Lillo, C., Iannone, A., Piazzolla, M., Badia, L., Piscaglia, F., Benevento, F., Serio, I., Castelli, F., Zaltron, S., Spinetti, A., Odolini, S., Bruno, R., Mondelli, M., Chessa, L., Loi, M., Torti, C., Costa, C., Mazzitelli, M., Pisani, V., Scaglione, V., Trecarichi, E. M., Zignego, A. L., Monti, M., Madia, F., Attala, L., Pierotti, P., Salomoni, E., Mariabelli, E., Santantonio, T. A., Bruno, S. R., Cela, E. M., Bassetti, M., Mazzarello, G., Alessandrini, A. I., Biagio, A. D., Nicolini, L. A., Raimondo, G., Filomia, R., Aghemo, A., Meli, R., Lazzarin, A., Salpietro, S., Fracanzani, A. L., Fatta, E., Lombardi, R., Lampertico, P., Borghi, M., D'Ambrosio, R., Degasperi, E., Puoti, M., Baiguera, C., D'Amico, F., Vinci, M., Rumi, M. G., Zuin, M., Zermiani, P., Andreone, P., Caraceni, P., Guarneri, V., Villa, E., Bernabucci, V., Bristot, L., Paradiso, M. L., Migliorino, G., Gambaro, A., Lapadula, G., Spolti, A., Soria, A., Invernizzi, P., Ciaccio, A., Luca, M., Malinverno, F., Ratti, L., Amoruso, D. C., Pisano, F., Scarano, F., Staiano, L., Morisco, F., Cossiga, V., Gentile, I., Buonomo, A. R., Foggia, M., Zappulo, E., Federico, A., Dallio, M., Coppola, N., Sagnelli, C., Martini, S., Monari, C., Nardone, G., Sgamato, C., Chemello, L., Cavalletto, L., Sterrantino, D., Zanetto, A., Zanaga, P., Brancaccio, G., Craxi, A., Petta, S., Calvaruso, V., Crapanzano, L., Madonia, S., Cannizzaro, M., Bruno, E. M., Licata, A., Amodeo, S., Capitano, A. R., Ferrari, C., Negri, E., Orlandini, A., Pesci, M., Gulminetti, R., Pagnucco, L., Parruti, G., Stefano, P. D., Coco, B., Corsini, R., Garlassi, E., Andreoni, M., Teti, E., Cerva, C., Baiocchi, L., Grassi, G., Gasbarrini, A., Pompili, M., Siena, M. D., Taliani, G., Spaziante, M., Persico, M., Masarone, M., Aglitti, A., Calvanese, G., Anselmo, M., Leo, P. D., Marturano, M., and Saracco, G. M.
- Subjects
Male ,HCV genotypes ,Ethnic group ,Linked-to-care patient ,Comorbidity ,Hepacivirus ,Logistic regression ,medicine.disease_cause ,Comorbidities ,Direct acting antivirals ,HCV Cohort ,Linked-to-care patients ,Aged ,Antiviral Agents ,Coinfection ,Female ,Hepatitis C, Chronic ,Humans ,Italy ,Middle Aged ,Transients and Migrants ,0302 clinical medicine ,Medicine ,Chronic ,Gastroenterology ,virus diseases ,Hepatitis C ,Life evaluation ,030220 oncology & carcinogenesis ,Cohort ,030211 gastroenterology & hepatology ,Comorbiditie ,Human ,Hepatitis C virus ,Settore MED/12 - GASTROENTEROLOGIA ,03 medical and health sciences ,Disease severity ,Antiviral Agent ,Hepaciviru ,Hepatology ,business.industry ,Settore MED/09 - MEDICINA INTERNA ,medicine.disease ,digestive system diseases ,Direct acting antiviral ,business ,Demography - Abstract
Background: Direct-acting antivirals are highly effective for the treatment of hepatitis C virus (HCV) infection, regardless race/ethnicity. We aimed to evaluate demographic, virological and clinical data of HCV-infected migrants vs. natives consecutively enrolled in the PITER cohort. Methods: Migrants were defined by country of birth and nationality that was different from Italy. Mann-Whitney U test, Chi-squared test and multiple logistic regression were used. Results: Of 10,669 enrolled patients, 301 (2.8%) were migrants: median age 47 vs. 62 years, (p < 0.001), females 56.5% vs. 45.3%, (p < 0.001), HBsAg positivity 3.8% vs. 1.4%, (p < 0.05). Genotype 1b was prevalent in both groups, whereas genotype 4 was more prevalent in migrants (p < 0.05). Liver disease severity and sustained virologic response (SVR) were similar. A higher prevalence of comorbidities was reported for natives compared to migrants (p < 0.05). Liver disease progression cofactors (HBsAg, HIV coinfection, alcohol abuse, potential metabolic syndrome) were present in 39.1% and 47.1% (p > 0.05) of migrants and natives who eradicated HCV, respectively. Conclusion: Compared to natives, HCV-infected migrants in care have different demographics, HCV genotypes, viral coinfections and comorbidities and similar disease severity, SVR and cofactors for disease progression after HCV eradication. A periodic clinical assessment after HCV eradication in Italians and migrants with cofactors for disease progression is warranted.
- Published
- 2021
114. Detection of feline immunodeficiency virus p24 antigen and p24-specific antibodies by monoclonal antibody-based assays
- Author
-
Lombardi, S., Poli, A., Massi, C., and Abramo, F.
- Published
- 1994
- Full Text
- View/download PDF
115. Feline and Canine Cutaneous Lymphocytosis: Reactive Process or Indolent Neoplastic Disease?
- Author
-
Francesco Albanese, Francesca Abramo, Michele Marino, Maria Massaro, Laura Marconato, Lucia Minoli, Valeria Martini, Luca Aresu, Albanese F., Abramo F., Marino M., Massaro M., Marconato L., Minoli L., Martini V., and Aresu L.
- Subjects
PARR ,cat ,cutaneous lymphocytosis ,dog ,immunohistochemistry ,lymphoma ,skin ,General Veterinary ,Veterinary medicine ,Cutaneous lymphocytosi ,Article ,SF600-1100 - Abstract
Cutaneous lymphocytosis (CL) is an uncommon and controversial lymphoproliferative disorder described in dogs and cats. CL is generally characterized by a heterogeneous clinical presentation and histological features that may overlap with epitheliotropic lymphoma. Therefore, its neoplastic or reactive nature is still debated. Here, we describe clinicopathological, immunohistochemical, and clonality features of a retrospective case series of 19 cats and 10 dogs with lesions histologically compatible with CL. In both species, alopecia, erythema, and scales were the most frequent clinical signs. Histologically, a dermal infiltrate of small to medium-sized lymphocytes, occasionally extending to the subcutis, was always identified. Conversely, when present, epitheliotropism was generally mild. In cats, the infiltrate was consistently CD3+; in dogs, a mixture of CD3+ and CD20+ lymphocytes was observed only in 4 cases. The infiltrate was polyclonal in all cats, while BCR and TCR clonal rearrangements were identified in dogs. Overall, cats had a long-term survival (median overall survival = 1080 days) regardless of the treatment received, while dogs showed a shorter and variable clinical course, with no evident associations with clinicopathological features. In conclusion, our results support a reactive nature of the disease in cats, associated with prolonged survival; despite a similar histological picture, canine CL is associated with a more heterogeneous lymphocytic infiltrate, clonality results, and response to treatment, implying a more challenging discrimination between CL and CEL in this species. A complete diagnostic workup and detailed follow-up information on a higher number of cases is warrant for dogs.
- Published
- 2022
116. Identification of Histopathological Criteria for the Diagnosis of Canine Cutaneous Progressive Angiomatosis
- Author
-
Francesca Abramo, Marta Vascellari, Giada Colzi, Luca Pazzini, Francesco Albanese, Lara Olivieri, Claudia Zanardello, Claudia Salvadori, Giancarlo Avallone, Paola Roccabianca, Abramo F., Vascellari M., Colzi G., Pazzini L., Albanese F., Olivieri L., Zanardello C., Salvadori C., Avallone G., and Roccabianca P.
- Subjects
skin ,General Veterinary ,endothelium ,progressive angiomatosi ,dog ,vessel ,progressive angiomatosis ,vessels - Abstract
The term angiomatosis is used to denote a group of well-known to poorly characterized proliferative vascular entities. In animals, cutaneous progressive angiomatosis (CPA) is a disorder with variable prognosis related to the extension and depth of infiltration of the surrounding tissues by vessels. CPA may share some microscopical features with other vascular proliferations such as low-grade well-differentiated capillaritic hemangiosarcoma (HS), making the diagnosis not always straightforward, especially in small biopsies. The aim of this study is to retrospectively assess the most common diagnostic microscopical features of CPA in dogs. In this work, 11 histopathological criteria were analyzed on 31 CPA and 11 primary cutaneous HS in dogs. Features significantly associated with CPA included: lobular growth, interposition of connective tissue and adnexa between the vascular proliferation, presence of nerve fibers, and a mixed vascular proliferative component. Absence of plump/prominent endothelial cells, lack of atypia, and lack of mitoses were also significant factors differentiating CPA from HS. Additional distinctive findings in CPA, although with no statistical association to CPA diagnosis, were vascular shunting, absence of necrosis, and endothelial cell piling up. In conclusion, the combined use of different microscopical clues allowed for the distinction of CPA from HS and was considered useful for the diagnosis of CPA.
- Published
- 2022
117. Histopathological Classification of Canine Cutaneous Round Cell Tumors Using Deep Learning: A Multi-Center Study
- Author
-
Massimo Salvi, Filippo Molinari, Selina Iussich, Luisa Vera Muscatello, Luca Pazzini, Silvia Benali, Barbara Banco, Francesca Abramo, Raffaella De Maria, Luca Aresu, Salvi M., Molinari F., Iussich S., Muscatello L.V., Pazzini L., Benali S., Banco B., Abramo F., De Maria R., and Aresu L.
- Subjects
medicine.medical_specialty ,computer-aided image analysi ,040301 veterinary sciences ,Mast cell tumors ,0403 veterinary science ,03 medical and health sciences ,cutaneous round cell tumors ,Round cell ,Medicine ,computer-aided image analysis ,030304 developmental biology ,Original Research ,0303 health sciences ,cutaneous round cell tumor ,lcsh:Veterinary medicine ,General Veterinary ,business.industry ,Deep learning ,Digital pathology ,deep learning ,04 agricultural and veterinary sciences ,mast cell tumors ,Fully automated ,Test set ,Multi center study ,dog ,lcsh:SF600-1100 ,Veterinary Science ,Radiology ,Artificial intelligence ,business ,digital pathology - Abstract
Canine cutaneous round cell tumors (RCT) represent one of the routine diagnostic challenges for veterinary pathologists. Computer-aided approaches are developed to overcome these restrictions and to increase accuracy and consistency of diagnosis. These systems are also of high benefit reducing errors when a large number of cases are screened daily. In this study we describe ARCTA (Automated Round Cell Tumors Assessment), a fully automated algorithm for cutaneous RCT classification and mast cell tumors grading in canine histopathological images. ARCTA employs a deep learning strategy and was developed on 416 RCT images and 213 mast cell tumors images. In the test set, our algorithm exhibited an excellent classification performance in both RCT classification (accuracy: 91.66%) and mast cell tumors grading (accuracy: 100%). Misdiagnoses were encountered for histiocytomas in the train set and for melanomas in the test set. For mast cell tumors the reduction of a grade was observed in the train set, but not in the test set. To the best of our knowledge, the proposed model is the first fully automated algorithm in histological images specifically developed for veterinary medicine. Being very fast (average computational time 2.63 s), this algorithm paves the way for an automated and effective evaluation of canine tumors.
- Published
- 2021
118. Evaluation of duodenal endoscopic and histologic findings, including counts of forkhead box P3-positive regulatory T cells, in dogs with immunosuppressant-responsive enteropathy
- Author
-
Pietro Ruggiero, Veronica Marchetti, Elena Benvenuti, Francesca Abramo, E. Bottero, Alessio Pierini, Marco Pietra, Eleonora Gori, Silvia Benali, Benvenuti E., Pierini A., Benali S.L., Gori E., Abramo F., Bottero E., Pietra M., Ruggiero P., and Marchetti V.
- Subjects
medicine.medical_specialty ,General Veterinary ,Animal ,business.industry ,Duodenum ,Inflammatory Bowel Diseases ,General Medicine ,medicine.disease ,Gastroenterology ,T-Lymphocytes, Regulatory ,Immunosuppressive Agent ,medicine.anatomical_structure ,Dogs ,Internal medicine ,Dog ,medicine ,Forkhead Box ,Animals ,Enteropathy ,Dog Diseases ,Dog Disease ,business ,Immunosuppressive Agents - Abstract
OBJECTIVE To prospectively evaluate the clinical and prognostic importance of duodenal endoscopic and histologic findings, including duodenal mucosal counts of forkhead box P3-positive regulatory T cells (Foxp3+ Tregs), in dogs with immunosuppressant-responsive enteropathy (IRE). ANIMALS 57 client-owned dogs with IRE. PROCEDURES The canine chronic enteropathy clinical activity index (CCECAI) was used to assess each dog when IRE was diagnosed (T0) and 1, 3, 6, and 12 months later. Dogs were grouped on the basis of clinical response (responder group vs nonresponder group) and 12-month long-term outcome (responded to treatment and did not relapse [good outcome group] vs did not respond to treatment or had relapsed [bad outcome group]). At T0, dogs underwent gastrointestinal endoscopy and endoscopic biopsy, with results for variables of duodenal endoscopic and histologic evaluations scored and compared across groups. RESULTS At T0, the overall median CCECAI score was 7; CCECAI score was not associated with clinical response or relapse. Dogs had significantly greater odds of being in the bad outcome group (vs the good outcome group) if they had a histologic score of 3 (OR, 3.5; 95% CI, 1.09 to 11.3). No differences in the counts of Foxp3+ Tregs were detected between groups. CONCLUSIONS AND CLINICAL RELEVANCE In dogs with IRE, results indicated that evaluation of Foxp3+ Tregs did not have prognostic value, whereas a duodenal histologic score of 3 could be a negative prognostic factor for response and relapse, and higher severity scores for intraepithelial lymphocytes and lamina propria lymphocytes and plasma cells in duodenal biopsy samples may be negatively associated with response.
- Published
- 2021
119. Hepatic lead and copper concentrations in dogs with chronic hepatitis and their relationship with hematology, serum biochemistry, and histopathology
- Author
-
Valentina Meucci, Luisa Vera Muscatello, Eleonora Gori, Alessio Pierini, Francesca Abramo, Veronica Marchetti, Gori E., Pierini A., Meucci V., Abramo F., Muscatello L.V., and Marchetti V.
- Subjects
medicine.medical_specialty ,Necrosis ,040301 veterinary sciences ,Veterinary medicine ,canine ,Standard Article ,030204 cardiovascular system & hematology ,liver ,Gastroenterology ,0403 veterinary science ,03 medical and health sciences ,0302 clinical medicine ,Dogs ,Chronic hepatitis ,Fibrosis ,Retrospective Studie ,Internal medicine ,SF600-1100 ,medicine ,Dog ,Animals ,biochemistry ,Dog Diseases ,heavy metals ,Retrospective Studies ,Hepatitis, Chronic ,Hematology ,General Veterinary ,Hepatology ,business.industry ,hematology ,Animal ,Microcytosis ,Histology ,04 agricultural and veterinary sciences ,heavy metal ,medicine.disease ,Standard Articles ,Serum biochemistry ,histopathology ,Histopathology ,SMALL ANIMAL ,medicine.symptom ,Dog Disease ,business ,Copper - Abstract
Background: Although the influence of copper ([Cu]) on chronic hepatitis (CH) has been widely studied in dogs, little information is available about the accumulation of other metals. Hypothesis/Objectives: We assessed the concentration of lead ([Pb]) in the livers of dogs with CH with or without abnormal hepatic [Cu] to establish if any association existed between [Pb] and either hematologic or biochemical variables, fibrosis, necrosis and inflammation of the liver on histology. Animals: Thirty-four dogs with CH that had hepatic [Cu] and [Pb] determined. Methods: Retrospective review of medical records of dogs with CH and hepatic [Cu] and [Pb]. Chronic hepatitis was defined using current American College of Veterinary Internal Medicine consensus statement guidelines. Hepatic [Cu] and [Pb] were determined using square wave anodic stripping voltammetry. Dogs were divided into 2 groups based on [Cu]
- Published
- 2021
120. Regressing Multiple Viral Plaques and Skin Fragility Syndrome in a Cat Coinfected with FcaPV2 and FcaPV3
- Author
-
Chiara Caporali, Laura Gallina, Alessandra Scagliarini, Francesca Abramo, Federica Savini, Gessica Tore, Alberto Alberti, Alberti A., Tore G., Scagliarini A., Gallina L., Savini F., Caporali C., and Abramo F.
- Subjects
N.A ,Mild Dysplasia ,medicine.medical_specialty ,Pathology ,CATS ,lcsh:Veterinary medicine ,General Veterinary ,integumentary system ,business.industry ,Spontaneous remission ,medicine.disease ,Koilocyte ,Immunology ,medicine ,lcsh:SF600-1100 ,Histopathology ,business ,Skin fragility syndrome ,Stomatitis ,Glucocorticoid ,medicine.drug - Abstract
Feline viral plaques are uncommon skin lesions clinically characterized by multiple, often pigmented, and slightly raised lesions. Numerous reports suggest thatpapillomaviruses(PVs) are involved in their development. Immunosuppressed and immunocompetent cats are both affected, the biological behavior is variable, and the regression is possible but rarely documented. Here we report a case of a FIV-positive cat with skin fragility syndrome and regressing multiple viral plaques in which the contemporary presence of two PV types (FcaPV2 and FcaPV3) was demonstrated by combining a quantitative molecular approach to histopathology. The cat, under glucocorticoid therapy for stomatitis and pruritus, developed skin fragility and numerous grouped slightly raised nonulcerated pigmented macules and plaques with histological features of epidermal thickness, mild dysplasia, and presence of koilocytes. Absolute quantification of the viral DNA copies (4555 copies/microliter of FcaPV2 and 8655 copies/microliter of FcaPV3) was obtained. Eighteen months after discontinuation of glucocorticoid therapy skin fragility and viral plaques had resolved. The role of the two viruses cannot be established and it remains undetermined how each of the viruses has contributed to the onset of VP; the spontaneous remission of skin lesions might have been induced by FIV status change over time due to glucocorticoid withdraw and by glucocorticoids withdraw itself.
- Published
- 2015
121. Malignant pilomatricoma with multiple bone metastases in a dog: Histological and immunohistochemical study
- Author
-
Francesca Abramo, Luigi Navas, Leonardo Meomartino, Manuela Martano, Brunella Restucci, Paola Maiolino, Lorenzo Lo Muzio, Martano, Manuela, Navas, Luigi, Meomartino, Leonardo, Abramo, F, Restucci, Brunella, Maiolino, Paola, and Muzio, Ll
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,business.industry ,Cancer ,Bone metastasis ,Articles ,General Medicine ,medicine.disease ,malignant pilomatricoma ,Lesion ,medicine.anatomical_structure ,Malignant pilomatricoma ,Immunology and Microbiology (miscellaneous) ,Lameness ,dog ,immunohistochemistry ,Medicine ,Immunohistochemistry ,Humerus ,Cortical bone ,medicine.symptom ,business - Abstract
An eleven year-old mongrel dog was referred with a history of left forelimb lameness and an ulcerated mass on the neck. Histologically, the cutaneous neoplasm revealed cystic lobules composed of basaloid cells with abrupt transition to central keratotic material, containing pycnotic and shadow cells. Approximately 3 months after primary diagnosis, a lesion of the cortical bone on the left humerus was observed using X-ray. Samples obtained from the humerus were processed for histopathological examination and the neoplastic tissue was observed to be similar to the type identified in the neck. Based on these findings, the tumor was diagnosed as a malignant pilomatricoma (MP) with bone metastasis. MP is a rare skin tumor that originates from hair matrix cells. To date, only nine reports have been presented in dogs. In the present study, we discuss the cytological and histological patterns of MP, confirmed by immunohistochemistry using β catenin antibody.
- Published
- 2013
122. Adjuvant gemcitabine after surgical removal of aggressive malignant mammary tumours in dogs
- Author
-
Francesca Abramo, Rm Lorenzo, Alessandra Ratto, Laura Marconato, Eric Zini, Marconato, L, Lorenzo, R M, Abramo, F, Ratto, A, Zini, E, and University of Zurich
- Subjects
Oncology ,medicine.medical_specialty ,Antimetabolites, Antineoplastic ,10253 Department of Small Animals ,3400 General Veterinary ,medicine.medical_treatment ,Mammary Neoplasms, Animal ,Deoxycytidine ,Dogs ,Internal medicine ,medicine ,Animal mortality ,Dog ,Animals ,Dog Diseases ,Prospective Studies ,Infusions, Intravenou ,Neoplasm Metastasis ,Stage (cooking) ,Infusions, Intravenous ,Prospective cohort study ,Chemotherapy ,630 Agriculture ,General Veterinary ,business.industry ,Animal ,Gemcitabine ,Combined Modality Therapy ,Clinical trial ,Neoplasm Metastasi ,Prospective Studie ,Neutering ,Treatment Outcome ,Chemotherapy, Adjuvant ,Quality of Life ,570 Life sciences ,biology ,Female ,Dog Disease ,business ,Adjuvant ,medicine.drug - Abstract
Canine mammary tumours are generally treated with surgery alone, despite the fact that 50% of them are malignant and many will eventually lead to recurrence or metastases. A prospective clinical trial in which dogs with aggressive mammary carcinoma of clinical stages IV and V were treated with surgical excision (n = 9) or with surgery and adjuvant weekly gemcitabine (n = 10) for at least four cycles was conducted. Gemcitabine was given as an intravenous infusion at the dose of 800 mg m(-2). Aim of the study was to explore potential beneficial effects of gemcitabine on time to local recurrence (TTR), time to distant metastases (TTM) and overall survival (OS) in canine patients with operated mammary tumours bearing high risk for locoregional failure and distant metastases. Also, factors associated with OS, including neutering status, body weight, age, clinical stage at presentation, tumour size, histological grade and, in dogs receiving chemotherapy, the number of gemcitabine treatments, were investigated. Finally, acute toxicities related to chemotherapy and quality of life were assessed in dogs receiving gemcitabine. Dogs treated with surgery alone or surgery followed by gemcitabine had no difference in TTR, TTM or OS (P > 0.05). In the group of dogs receiving adjuvant chemotherapy, the number of gemcitabine treatments was positively correlated with OS (P = 0.017). Gemcitabine treatment was well tolerated, with no dogs experiencing clinically relevant haematological or gastrointestinal toxicity. Despite being safe at the present dose, gemcitabine chemotherapy as an adjunct treatment to surgical excision may not be recommended in dogs with aggressive mammary carcinoma.
- Published
- 2008
- Full Text
- View/download PDF
123. Paraneoplastic alopecia associated with hepatocellular carcinoma in a cat
- Author
-
L. Marconato, Paolo Viacava, Veronica Marchetti, Francesco Albanese, Francesca Abramo, Marconato L., Albanese F., Viacava P., Marchetti V., and Abramo F.
- Subjects
Thorax ,Pathology ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Paraneoplastic Syndromes ,Cat Diseases ,Diagnosis, Differential ,Carcinoma ,medicine ,Animals ,Depression (differential diagnoses) ,CATS ,General Veterinary ,Animal ,business.industry ,Liver Neoplasms ,Alopecia ,Cat ,Cat Disease ,medicine.disease ,medicine.anatomical_structure ,Hair loss ,Liver Neoplasm ,Hepatocellular carcinoma ,Cats ,Abdomen ,Female ,Spayed Female ,business - Abstract
A 15-year-old spayed female domestic shorthair cat presented with alopecia associated with hepatocellular carcinoma. Clinical signs, which had commenced 6 months previously, included loss of appetite, loss of weight, and depression. As reported by the owner, the cat developed alopecia a week before referral. The hair loss was localized to the ventral aspect of the thorax and abdomen, medial aspect of front and hind limbs, and ventral aspect of the tail, and was associated with histological features consistent with paraneoplastic alopecia. At necropsy, multiple hepatic nodules were observed, and subsequent histopathological investigation showed cords and sheets of hepatocyte-like neoplastic cells positive for the hepatocyte marker (Hep Par 1), thereby demonstrating the hepatocellular origin of the tumour, which was diagnosed as a hepatocellular carcinoma. This is the first report of feline paraneoplastic alopecia associated with hepatocellular carcinoma confirmed by the Hep Par 1 marker.
- Published
- 2007
124. Construction and evaluation of sponge scaffolds from hyaluronic acid derivatives for potential cartilage regeneration
- Author
-
Mario Giorgi, Francesca Abramo, Gaetano Giammona, Calogero Fiorica, Giovanna Pitarresi, Fabio Salvatore Palumbo, Alessandro Gulino, Mauro Di Stefano, Palumbo, FS, Fiorica, C, Pitarresi, G, Giorgi, M, Abramo, F, Gulino, A, Di Stefano, M, and Giammona, G
- Subjects
Materials science ,Biomedical Engineering ,Settore MED/08 - Anatomia Patologica ,Glycosaminoglycan ,chemistry.chemical_compound ,Physiological condition ,Hyaluronidase ,Chemical derivatization ,Hyaluronic acid ,medicine ,Organic chemistry ,General Materials Science ,Hydrophobic and hydrophilic ,Derivatization ,biology ,Cartilage ,Bovine chondrocyte ,General Chemistry ,General Medicine ,biology.organism_classification ,Hyaluronic acid derivative ,Receptor interaction ,Sponge ,medicine.anatomical_structure ,chemistry ,Cartilage regeneration ,Settore CHIM/09 - Farmaceutico Tecnologico Applicativo ,Self-healing hydrogels ,Biological propertie ,Swelling ,medicine.symptom ,medicine.drug ,Nuclear chemistry - Abstract
A two or one pot synthesis has been used for the reaction of hyaluronic acid (HA) with octadecylamine (C-18) and hydrazine (Hy). In both cases, the chemical derivatization involved primary hydroxyl groups of hyaluronic acid and not its carboxyl groups, whose presence is important for receptor interaction. In this way, Hy-HA-C-18 derivatives have been obtained with appropriate hydrophobic and hydrophilic character. Their ability to form homogeneous physical hydrogels has been evaluated as well as the possibility to obtain porous sponges through salt leaching technology. Sponges showing the highest porosity, potentially compatible with cell entrapment, have been characterized with regard to their physicochemical and biological properties. Swelling ability under simulated physiological conditions and stability in the absence or in the presence of hyaluronidase have been investigated. Bovine chondrocytes were viable in Hy-HA-C-18 sponges as determined with MTS assay and were able to produce collagen and glycosaminoglycans, as assessed by using Masson's trichrome and Alcian blue, respectively. Finally, in vivo degradation of Hy-HA-C-18 sponges has been confirmed after subcutaneous implantation in mice until 6 weeks
- Published
- 2014
125. SPONGES OF NEW HYALURONIC ACID DERIVATIVES FOR BIOMEDICAL APPLICATIONS
- Author
-
Pitarresi, G., Palumbo, F. S., Fiorica, C., Mario Giorgi, Francesca Abramo, Gulino, A., Calascibetta, F., Giammona, G., Pitarresi, G, Palumbo, FS, Fiorica, C, Giorgi, M, Abramo, F, Gulino, A, Calascibetta, F, and Giammona, G
- Subjects
Hyaluronic acid, Sponges
126. Using colorimetric in situ hybridisation method for FcaPV-2 to estimate postsurgical prognosis in feline Bowenoid in situ carcinoma.
- Author
-
Abramo F, Mazzei M, Forzan M, Giannetti G, Albanese F, Melchiotti E, Zanna G, and Vascellari M
- Abstract
Background: Feline Bowenoid in situ carcinoma (BISC) is frequently associated with Felis catus papillomavirus-2 (FcaPV-2). Although surgical excision of BISC is expected to be curative, recurrent lesions are reported and it is not known whether it is a consequence of incomplete surgery or residual viral load., Objectives: To combine colorimetric in situ hybridisation (CISH) and quantitative (q)PCR for the detection of viral DNA, and to correlate the clinical outcome of cats with BISC in which FcaPV-2 DNA is detected at surgical margins., Animals: Twenty-seven cats with a histopathological diagnosis of BISC., Materials and Methods: Sections including core and margins of the lesions were used for histopathological evaluation, qPCR and CISH. After surgical removal of the lesion, clinical follow-up data were recorded for 6 months., Results: Six of 12 cases in which all four histological margins were evaluable were used to correlate the infection status at the margins with the follow-up data. Four showed margin positivity, of which half relapsed as expected and half cured; two cases were negative, of which one cured as expected while the other relapsed. Fifteen cases where only three, two or one of the histological margins were evaluable, were considered to adequately correlate the status of infection with the follow-up data if CISH was positive. Follow-up data were available for three with positive margins: one relapsed while the other two were cured., Conclusions and Clinical Relevance: Wide clinical surgical margins are always recommended for neoplastic conditions, yet there was no evidence that CISH margin examination would be beneficial in predicting recurrence in this viral-induced lesion., (© 2024 The Author(s). Veterinary Dermatology published by John Wiley & Sons Ltd on behalf of ESVD and ACVD.)
- Published
- 2024
- Full Text
- View/download PDF
127. Dermoscopic findings and comparison of usefulness of longitudinal versus transversal sections in the histological diagnosis of alopecia X.
- Author
-
Zanna G, Abramo F, Contiero B, Zini E, Albanese F, Borio E, Godizzi F, Necci F, Luciani L, and Roccabianca P
- Subjects
- Animals, Dogs, Hair pathology, Hair Follicle diagnostic imaging, Hair Follicle pathology, Skin pathology, Alopecia diagnosis, Alopecia veterinary, Alopecia pathology, Darier Disease pathology, Darier Disease veterinary
- Abstract
Background: A combination of dermoscopic and histological findings may provide useful information for the diagnosis of hair follicle diseases. However, there are no studies on dermoscopic-histopathological correlations in dogs affected by alopecia X, and comparison of longitudinal versus transversal sectioning of skin biopsy specimens in the assessment of this hair loss disorder has not been thoroughly investigated., Hypothesis/objectives: The aim of this study was to correlate dermoscopic and histological features using both longitudinal and transversal sectioning of skin biopsy samples to gain additional information for the diagnosis of alopecia X., Animals: Nineteen Pomeranian dogs affected by alopecia X and five healthy Pomeranians as controls., Materials and Methods: Dermoscopic-histological correlation was performed within the diseased group, whereas histological comparisons against controls. The demographic and clinical characteristics also were related to the histological findings., Results: The dermoscopic findings revealed scattered, thinned, short hairs mixed with amorphous keratoseborrhoeic-like material (follicular plugging), perifollicular and intrafollicular scaling, and hyperpigmentation varying from pinpoint black spots to a diffuse texture. Dermoscopic findings correlated with histological findings for selected qualitative and quantitative findings. The usefulness of transversal sections was demonstrated in accurately determining the hair follicular density and counts, growth arrest phases and in identifying mineralisation of hair follicle basement membrane when compared to the longitudinal. Conversely, no correlations between histological findings and demographic and clinical characteristics were detected., Conclusions and Clinical Relevance: These data provide evidence of the usefulness of dermoscopic evaluation as an accessory diagnostic tool and of transversal sections of skin biopsies as complementary to the diagnosis of alopecia X., (© 2023 ESVD and ACVD.)
- Published
- 2024
- Full Text
- View/download PDF
128. Clinical, histopathological, and molecular characterization of canine pigmented viral plaques.
- Author
-
Orlandi M, Mazzei M, Albanese F, Pazzini L, Mei M, Lazzarini G, Forzan M, Massaro M, Vascellari M, and Abramo F
- Subjects
- Humans, Dogs, Animals, Retrospective Studies, Cohort Studies, Dog Diseases diagnosis, Dog Diseases epidemiology, Dog Diseases pathology, Papillomavirus Infections epidemiology, Papillomavirus Infections veterinary, Papillomavirus Infections pathology, Carcinoma, Squamous Cell veterinary, Parvovirus, Canine, Parvoviridae Infections veterinary
- Abstract
Canine pigmented viral plaques (PVPs) are proliferative epidermal lesions caused by canine papillomaviruses (CPVs). Although the lesions are benign, neoplastic transformation has been reported. Cases reported in the literature are few and mainly focused on genome sequencing. The aim of this study was to collect data on the epidemiology, clinicopathological features, and genotyping of PVPs. Fifty-five canine PVPs were retrospectively retrieved and histologically evaluated. Follow-up was available for 33 cases. The median age was 6.5 years and pugs were the most represented breed (25%). There were 4 clinical presentations: a single lesion (24%), multiple lesions (75%) in one (41%) or different sites (34%), and generalized lesions all over the body (24%). The abdomen and axillae were the most common sites. In single lesions, no recurrence was observed after conventional surgery, whereas different medical treatments reported for multiple lesions were not successful. Spontaneous regression was reported in 3 cases. Neoplasia in contiguity with PVPs was seen in 5 of 55 lesions (9%), and 1 dog was euthanized due to invasive squamous cell carcinoma (SCC). The most useful histopathological features for diagnosis were scalloped profile, epidermal spikes, hypergranulosis, and hyperpigmentation. L1 immunolabeling was present in 14 of 16 cases (87%). Sequencing revealed that 10 of 16 cases were associated with CPV-9 (71%), 2 cases were associated with CPV-4 (14%), and 2 cases were associated with CPV-8 (14%). In conclusion, this represents a large cohort study on canine PVPs reporting data on clinicopathological features, therapy, outcome, and the type of CPV involved for the first time in Italy., Competing Interests: Declaration of Conflicting InterestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2023
- Full Text
- View/download PDF
129. Eumycetoma Caused by Madurella pseudomycetomatis in a Captive Tiger ( Panthera tigris ).
- Author
-
Orlandi M, Giglia G, Danesi P, Laricchiuta P, and Abramo F
- Abstract
A captive-kept adult male tiger presented with a large cutaneous and subcutaneous mass on the thigh with a fistula. During sedation, multiple nodules were detected and samples for a histopathological exam were collected. Histologically, granulomatous panniculitis and dermatitis were seen around dense aggregates of pigmented fungal hyphae, and a diagnosis of phaeohyphomycosis was made; considering the clinical features, it was classified as a eumycotic mycetoma. This is a rarely reported subcutaneous fungal infection in humans and animals, caused by dematiaceous fungi. Clinically, it is characterized by tumefaction, fistulous sinus tracts, and the formation of macroscopically visible grains. In the literature, only a few infections in wild felids have been reported. In this case, Fontana-Masson staining better showed pigmentation and panfungal PCR and sequencing identified Madurella pseudomyectomatis (OP623507) as the causative agent. Systemic therapy with oral administration of itraconazole was planned, but the patient died during the first period of treatment. The animal was not submitted for post-mortem examination. Visceral dissemination of the agent cannot be excluded. To the authors' knowledge, this is the first report of eumycotic mycetoma by Madurella pseudomycetomatis in a captive tiger.
- Published
- 2022
- Full Text
- View/download PDF
130. Skin Photodamage Lesions in a Bilateral Feline Auricular Primary Fibrosarcoma.
- Author
-
Parisi F, Abramo F, Maimone M, Poli A, and Millanta F
- Abstract
As with human species, recent studies also suggest a photoinduced etiopathology for non-epithelial cutaneous tumors in feline species. We report a recent case of a ten-year-old male cat with a white-hair coat and mesenchymal neoplasms of both auricles. Cytology, complete blood count (CBC), serum biochemistry and imaging examinations were performed. After surgery, the samples underwent routinary histopathology and were additionally stained with orcein. A routine analysis yielded values within a normal range and the imaging examination showed no abnormalities, suggesting that the bilateral presentation of neoplasms was primary rather than metastatic. The cytology was inconclusive, but, through histopathology, two well-differentiated fibrosarcomas were diagnosed and histopathological changes related to chronic UV exposure (such as epidermal hyperplasia, stratification disorders, keratinocyte dysplasia and an accumulation of elastotic material) were documented in the skin adjacent to the lesions. An orcein stain succeeded in highlighting elastosis. The elastic fibers lost their regular structure and orientation and appeared to be fragmented, wavy to branched and knotted. A morphometric analysis showed that the amount of elastotic material in the dermis close to the tumors was more than double compared with the more distant areas. Elastosis is considered to be a hallmark of photodamage; thus, an involvement of UV rays in the carcinogenic process of the tumors may be suspected.
- Published
- 2022
- Full Text
- View/download PDF
131. Serum Amino Acids Imbalance in Canine Chronic Hepatitis: Results in 16 Dogs.
- Author
-
Habermaass V, Gori E, Abramo F, Bartoli F, Pierini A, Mariti C, Lippi I, and Marchetti V
- Abstract
In humans, chronic liver disease may cause alterations in amino acids (AAs) metabolism, with serum branched-chain AAs (BCAAs) decreasing and aromatic AAs (AAAs) increasing. A reduced Fischer ratio (BCAAs/AAAs) has been found to be associated with hepatic fibrosis and is useful for assessing prognosis in human patients. In veterinary medicine, few studies have been performed, and in contrast to human patients, dogs with different kinds of hepatopathy tend to show both increased AAAs and BCAAs. In dogs, the association between histological scores and serum AAs has not been previously investigated. The aim of this study was to evaluate serum AAs in dogs with chronic hepatitis (CH) compared with a healthy control group (C) and, among CH dogs, in relation to their histological fibrosis and necroinflammatory activity scores. Leftover serum samples of 16 dogs with histological CH and 25 healthy dogs were employed. Serum AAs were measured by high performance liquid chromatography. Proline and the AAAs phenylalaine and tyrosine progressively increased with the histological severity. In contrast, cysteine, tryptophan and BCAA isoleucine progressively reduced. Lysine and the BCAAs leucine and valine showed a non-linear trend with the histological findings. The BCAAs/AAAs ratio was significatively reduced if higher grades of liver fibrosis were present.
- Published
- 2022
- Full Text
- View/download PDF
132. Identification of Histopathological Criteria for the Diagnosis of Canine Cutaneous Progressive Angiomatosis.
- Author
-
Abramo F, Vascellari M, Colzi G, Pazzini L, Albanese F, Olivieri L, Zanardello C, Salvadori C, Avallone G, and Roccabianca P
- Abstract
The term angiomatosis is used to denote a group of well-known to poorly characterized proliferative vascular entities. In animals, cutaneous progressive angiomatosis (CPA) is a disorder with variable prognosis related to the extension and depth of infiltration of the surrounding tissues by vessels. CPA may share some microscopical features with other vascular proliferations such as low-grade well-differentiated capillaritic hemangiosarcoma (HS), making the diagnosis not always straightforward, especially in small biopsies. The aim of this study is to retrospectively assess the most common diagnostic microscopical features of CPA in dogs. In this work, 11 histopathological criteria were analyzed on 31 CPA and 11 primary cutaneous HS in dogs. Features significantly associated with CPA included: lobular growth, interposition of connective tissue and adnexa between the vascular proliferation, presence of nerve fibers, and a mixed vascular proliferative component. Absence of plump/prominent endothelial cells, lack of atypia, and lack of mitoses were also significant factors differentiating CPA from HS. Additional distinctive findings in CPA, although with no statistical association to CPA diagnosis, were vascular shunting, absence of necrosis, and endothelial cell piling up. In conclusion, the combined use of different microscopical clues allowed for the distinction of CPA from HS and was considered useful for the diagnosis of CPA.
- Published
- 2022
- Full Text
- View/download PDF
133. Serum protein electrophoresis in 26 dogs with chronic hepatitis.
- Author
-
Gori E, Pierini A, Tulone F, Abramo F, and Marchetti V
- Subjects
- Animals, Blood Proteins analysis, Dogs, Electrophoresis veterinary, Hepatitis, Chronic diagnosis, Hepatitis, Chronic veterinary, Retrospective Studies, gamma-Globulins, Dog Diseases diagnosis, Globulins
- Abstract
Serum protein electrophoresis (SPE) shows the distribution of protein fractions, helping clinicians to characterize some pathologic processes. Information is lacking in the literature about SPE alterations in dogs with chronic hepatitis (CH). Our aim was to describe SPE alterations in canine CH, to compare SPE results to histologic scores, and to study SPE trends during follow-up. We reviewed retrospectively case data from dogs with a histologic diagnosis of CH. Only cases with SPE, CBC, and serum chemistry results available were included. Dogs were divided into subgroups based on histologic necroinflammatory activity (A) and fibrosis (F) scores (groups A0-1 and A>1; groups F<2 and F≥2). We included 26 dogs; 15 had follow-up SPE. The most common SPE alterations at admission were hypoalbuminemia ( n = 16), increases in α1-globulins ( n = 11), γ-globulins ( n = 11), α2-globulins ( n = 8), β2-globulins ( n = 7), and β1-globulins ( n = 6), and decreased albumin:globulin (A:G) ratios ( n = 20). Four of 11 dogs had β-γ bridging. Groups with higher A and F scores had higher β2-globulins. Eleven of 15 dogs with a post-treatment SPE had a decrease in γ-globulins and increase in A:G ratio compared to their T0, although there was no statistically significant difference. Although further studies are warranted, SPE may be useful for monitoring canine CH.
- Published
- 2022
- Full Text
- View/download PDF
134. Trehalose Treatment in Zebrafish Model of Lafora Disease.
- Author
-
Della Vecchia S, Ogi A, Licitra R, Abramo F, Nardi G, Mero S, Landi S, Battini R, Sicca F, Ratto GM, Santorelli FM, and Marchese M
- Subjects
- Animals, Mutation, Protein Tyrosine Phosphatases, Non-Receptor genetics, Protein Tyrosine Phosphatases, Non-Receptor metabolism, Seizures, Trehalose pharmacology, Ubiquitin-Protein Ligases genetics, Zebrafish metabolism, Lafora Disease drug therapy, Lafora Disease genetics, Lafora Disease metabolism
- Abstract
Mutations in the EPM2A gene encoding laforin cause Lafora disease (LD), a progressive myoclonic epilepsy characterized by drug-resistant seizures and progressive neurological impairment. To date, rodents are the only available models for studying LD; however, their use for drug screening is limited by regulatory restrictions and high breeding costs. To investigate the role of laforin loss of function in early neurodevelopment, and to screen for possible new compounds for treating the disorder, we developed a zebrafish model of LD. Our results showed the epm2a
-/- zebrafish to be a faithful model of LD, exhibiting the main disease features, namely motor impairment and neuronal hyperexcitability with spontaneous seizures. The model also showed increased inflammatory response and apoptotic death, as well as an altered autophagy pathway that occurs early in development and likely contributes to the disease progression. Early administration of trehalose was found to be effective for rescuing motor impairment and neuronal hyperexcitability associated with seizures. Our study adds a new tool for investigating LD and might help to identify new treatment opportunities.- Published
- 2022
- Full Text
- View/download PDF
135. Biomolecular Prospecting, Informative Gaps, and the Cancer Clinic: A Qualitative Fieldwork and an Epistemological, Historical and Ethical Analysis of Informed Consent for Clinical Trials for Monoclonal Antibodies and Biobank Research.
- Author
-
D'Abramo F, Bont A, and Nüßlein L
- Abstract
What happens to patients with cancer engaged in biomedical research when intellectual property regimes and ethical regimes intersect? This qualitative historical study addresses this question by situating the experiences, hopes, and reasons of patients to enter clinical trials within the historical trajectory of informed consent and monoclonal antibodies, the biotechnology underpinning many targeted drugs used in oncological clinical trials and biobank research. Based on fieldwork we undertook in a German university hospital where we interviewed patients and the medical personnel, a historical review, and an ethical analysis we inquire into the effects that financial, legal, and technological changes connected to the relevant pharmaceutical research and commerce have on cancer patients engaged in clinical trials and biobank research. We find that the controversial aspects of monoclonal antibodies, especially those related to the commercial interests at stake, enter the informed consent process mainly in the form of informative gaps. We highlight how a qualitative analysis of the clinic, especially when it is situated against the backdrop of the history of related technological advancements and patent regime, it can serve the purpose of giving voice to subjects who are silenced by regimes of an ethical, epistemic, and commercial kind while pointing to informed consent as an unhelpful device for addressing risks arising from the commercial purposes of biomedical products and infrastructure., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 D’Abramo, Bont and Nüßlein.)
- Published
- 2022
- Full Text
- View/download PDF
136. Auricular Non-Epithelial Tumors with Solar Elastosis in Cats: A Possible UV-Induced Pathogenesis.
- Author
-
Millanta F, Parisi F, Poli A, Sorelli V, and Abramo F
- Abstract
The photoinduced etiopathology of actinic keratosis and squamous cell carcinoma in feline species is well known. This etiology has also been reported for non-epithelial cutaneous tumors in other species. To date, no cases of auricular non-epithelial cutaneous neoplasms erased in a contest of actinic keratosis in cats have been reported. The aim of this study was to describe feline auricular non-epithelial cutaneous neoplasms associated with typical UV-induced cutaneous lesions and solar elastosis. The study was conducted on five feline cases diagnosed with auricular non-epithelial cutaneous tumors (two fibrosarcomas, one mixosarcoma, one epithelioid melanoma and one hemangiosarcoma), selected from the Tumor Registry of the Department of Veterinary Sciences of the University of Pisa (1998-2018). Ten and six feline auricular biopsies of normal skin and skin with actinic keratosis, respectively, were used as controls. Orcein stain was used to investigate solar elastosis. Histological changes related to chronic solar irradiation were documented in the skin adjacent to the neoplastic lesions in the five cats. Considering the anatomical localization and the results of histopathology, this study suggests that non-epithelial cutaneous neoplasms may have a UV-induced etiopathogenesis in the feline species.
- Published
- 2022
- Full Text
- View/download PDF
137. Feline and Canine Cutaneous Lymphocytosis: Reactive Process or Indolent Neoplastic Disease?
- Author
-
Albanese F, Abramo F, Marino M, Massaro M, Marconato L, Minoli L, Martini V, and Aresu L
- Abstract
Cutaneous lymphocytosis (CL) is an uncommon and controversial lymphoproliferative disorder described in dogs and cats. CL is generally characterized by a heterogeneous clinical presentation and histological features that may overlap with epitheliotropic lymphoma. Therefore, its neoplastic or reactive nature is still debated. Here, we describe clinicopathological, immunohistochemical, and clonality features of a retrospective case series of 19 cats and 10 dogs with lesions histologically compatible with CL. In both species, alopecia, erythema, and scales were the most frequent clinical signs. Histologically, a dermal infiltrate of small to medium-sized lymphocytes, occasionally extending to the subcutis, was always identified. Conversely, when present, epitheliotropism was generally mild. In cats, the infiltrate was consistently CD3+; in dogs, a mixture of CD3+ and CD20+ lymphocytes was observed only in 4 cases. The infiltrate was polyclonal in all cats, while BCR and TCR clonal rearrangements were identified in dogs. Overall, cats had a long-term survival (median overall survival = 1080 days) regardless of the treatment received, while dogs showed a shorter and variable clinical course, with no evident associations with clinicopathological features. In conclusion, our results support a reactive nature of the disease in cats, associated with prolonged survival; despite a similar histological picture, canine CL is associated with a more heterogeneous lymphocytic infiltrate, clonality results, and response to treatment, implying a more challenging discrimination between CL and CEL in this species. A complete diagnostic workup and detailed follow-up information on a higher number of cases is warrant for dogs.
- Published
- 2022
- Full Text
- View/download PDF
138. Localization and genotyping of canine papillomavirus in canine inverted papillomas.
- Author
-
Orlandi M, Mazzei M, Vascellari M, Melchiotti E, Zanardello C, Verin R, Albanese F, Necci F, Pazzini L, Lazzarini G, and Abramo F
- Subjects
- Animals, DNA, Viral genetics, Dogs, Genotype, In Situ Hybridization veterinary, Papillomaviridae genetics, Dog Diseases, Papilloma, Inverted veterinary, Papillomavirus Infections veterinary
- Abstract
Numerous canine papillomaviruses (CPVs) have been identified (CPV1-23). CPV1, 2, and 6 have been associated with inverted papillomas (IPs). We retrieved 19 IPs from 3 histopathology archives, and evaluated and scored koilocytes, inclusion bodies, giant keratohyalin granules, cytoplasmic pallor, ballooning degeneration, and parakeratosis. IHC targeting major capsid proteins of PV was performed, and CPV genotyping was achieved by PCR testing. Tissue localization of CPV DNA and RNA was studied by chromogenic and RNAscope in situ hybridization (DNA-CISH, RNA-ISH, respectively). IPs were localized to the limbs (50%), trunk (30%), and head (20%), mainly as single nodules (16 of 19). In 15 of 19 cases, immunopositivity was detected within the nuclei in corneal and subcorneal epidermal layers. PCR revealed CPV1 in 11 IPs and CPV2 DNA in 3 IPs. Overall, 14 of 17 cases were positive by both DNA-CISH and RNA-ISH, in accord with PCR results. A histologic score >5 was always obtained in cases in which the viral etiology was demonstrated by IHC, DNA-CISH, and RNA-ISH. IHC and molecular approaches were useful to ascertain the viral etiology of IPs. Although IHC is the first choice for diagnostic purposes, ISH testing allows identification of PV type and the infection phase. RNA-ISH seems a promising tool to deepen our understanding of the pathogenesis of different PV types in animal species.
- Published
- 2021
- Full Text
- View/download PDF
139. Linear arrector pili muscle hamartoma on the tail of a sphynx cat.
- Author
-
Fanton N, Orlandi M, and Abramo F
- Subjects
- Animals, Cats, Dogs, Hair, Hair Follicle, Muscle, Smooth, Cat Diseases diagnosis, Dog Diseases diagnosis, Hamartoma diagnosis, Hamartoma veterinary
- Abstract
Arrector pili muscle (APM) hamartoma is reported in humans and dogs. We describe a linear APM hamartoma in a sphynx cat. The lesion was characterized by multiple nodules distributed linearly along the tail, made of randomly arranged hypertrophic smooth muscles, the size of which tended to wax-and-wane during a one year follow-up., (© 2021 ESVD and ACVD.)
- Published
- 2021
- Full Text
- View/download PDF
140. Hepatic lead and copper concentrations in dogs with chronic hepatitis and their relationship with hematology, serum biochemistry, and histopathology.
- Author
-
Gori E, Pierini A, Meucci V, Abramo F, Muscatello LV, and Marchetti V
- Subjects
- Animals, Copper, Dogs, Retrospective Studies, Dog Diseases, Hematology, Hepatitis, Chronic veterinary
- Abstract
Background: Although the influence of copper ([Cu]) on chronic hepatitis (CH) has been widely studied in dogs, little information is available about the accumulation of other metals., Hypothesis/objectives: We assessed the concentration of lead ([Pb]) in the livers of dogs with CH with or without abnormal hepatic [Cu] to establish if any association existed between [Pb] and either hematologic or biochemical variables, fibrosis, necrosis and inflammation of the liver on histology., Animals: Thirty-four dogs with CH that had hepatic [Cu] and [Pb] determined., Methods: Retrospective review of medical records of dogs with CH and hepatic [Cu] and [Pb]. Chronic hepatitis was defined using current American College of Veterinary Internal Medicine consensus statement guidelines. Hepatic [Cu] and [Pb] were determined using square wave anodic stripping voltammetry. Dogs were divided into 2 groups based on [Cu]: <400 ppm (LoCu) and ≥400 ppm (HiCu)., Results: The median [Cu] and [Pb] were 357 ppm (range, 100-7743 ppm) and 58.7 (range, 6.89-224.4 ppm), respectively. Nineteen dogs had LoCu and 15 dogs had HiCu. Median [Pb] was significantly higher in HiCu compared to LoCu dogs (P < .001). Hepatic [Pb] and [Cu] were significantly correlated (rho = 0.7; P < .001). Dogs with microcytosis had higher [Pb] than did dogs with normal red cell volume (P = .02). Hepatic [Pb] was not correlated with either necroinflammatory or fibrosis scores., Conclusions and Clinical Importance: Although additional studies are needed to better understand the clinical role of hepatic [Pb], dogs with abnormal hepatic [Cu] may also have higher hepatic [Pb]. In addition, in dogs with high hepatic [Pb], microcytosis may be present., (© 2021 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.)
- Published
- 2021
- Full Text
- View/download PDF
141. Gross and histological findings in the canine placenta and amnion at term: What's normal, abnormal or pathological?
- Author
-
Tesi M, Miragliotta V, Scala L, Aronica E, Lazzarini G, Fanelli D, Rota A, and Abramo F
- Subjects
- Amnion pathology, Animals, Animals, Newborn, Birth Weight, Dog Diseases pathology, Female, Male, Placenta pathology, Pregnancy, Amnion anatomy & histology, Dogs anatomy & histology, Placenta anatomy & histology
- Abstract
The canine placenta is an underexamined organ. Placental abnormalities can affect foetus development and may be responsible for a low weight of the infant at birth; however, knowledge on their clinical significance in the canine species is limited. We aimed to describe macroscopic and microscopic findings in the canine placenta and amnion at term in clinically uncomplicated pregnancies and to evaluate their relationship with birth weight of healthy puppies. During natural delivery or C-section, the birth weight of 82 puppies was recorded, 72 placentas and 66 amnions were recovered. The foetal and maternal surfaces of the placental girdle, marginal haematoma and amnion were evaluated. Each gross finding was recorded, morphometrically assessed and sampled for histological diagnosis. Furthermore, specimens of placenta and amnion were collected from representative areas and microscopic deviations from normal structure were evaluated in haematoxylin and eosin sections. Gross examination revealed 'abnormalities' in the 75.4% of the collected placentas. Necrosis was the gross change most commonly observed in the placental girdle (72.5%). Congestion (17.4%) and clotted blood/fibrinoid material (2.9%) were also observed. No gross changes of either the marginal haematoma or the amnion were recorded. Histologically, placental girdle showed necrosis (62.3%), mineralization (52.2%), congestion (36.2%) and neutrophilic infiltration (27.5%). Marginal haematoma exhibited mineralization (11.6%) and neutrophils (29%), while necrotic foci were rarely observed (4.3%). In the amnion, the most frequent alteration observed was hypertrophy of the epithelium (35.9%) followed by oedema (31.2%), mineralized foci (28.1%), fibrosis (23.4%), congestion (15.6%) and more rarely neutrophils (12.5%). Puppies' birth weight was not statistically affected by either gross or histological abnormalities. Our study revealed that macroscopic and microscopic 'abnormalities' of the placenta and amnion may be common in uncomplicated pregnancies at term; however, no implications on puppies' birth weight were observed. Deviations from 'normal' morphology of canine foetal adnexa warrant further investigation to assess their clinical implications if present., (© 2021 Wiley-VCH GmbH.)
- Published
- 2021
- Full Text
- View/download PDF
142. The past and present of pandemic management: health diplomacy, international epidemiological surveillance, and COVID-19.
- Author
-
D'Abramo F
- Subjects
- Cholera epidemiology, Cholera history, Cholera prevention & control, Diplomacy history, History, 19th Century, History, 20th Century, Humans, Pandemics prevention & control, COVID-19 epidemiology, COVID-19 prevention & control, Epidemiological Monitoring, Global Health history, International Cooperation history, Pandemics history, Population Surveillance
- Abstract
The establishment of international sanitary institutions, which took place in the context of rivalry among the great European powers and their colonial expansion in Asia, allowed for the development of administrative systems of international epidemiological surveillance as a response to the cholera epidemics at the end of the nineteenth century. In this note, I reflect on how a historical analysis of the inception of international epidemiological surveillance and pandemic management helps us to understand what is happening in the COVID-19 pandemic today.
- Published
- 2021
- Full Text
- View/download PDF
143. Skin Lesions in Feline Leishmaniosis: A Systematic Review.
- Author
-
Abramo F, Albanese F, Gattuso S, Randone A, Fileccia I, Dedola C, Ibba F, Ottaiano P, and Brianti E
- Abstract
Feline leishmaniosis (FeL) is increasingly reported throughout the world and skin lesions predominate in the clinical picture. There are, however, few evidence-based data on cutaneous feline leishmaniosis and directions are strongly needed for a better management of the disease. In this study, we systematically reviewed what is currently known about the clinical dermatological presentation of FeL through analysis of the literature and, further, by adding unpublished cases managed by Italian veterinary dermatologists. Sixty-six feline cases of cutaneous leishmaniosis published in 33 articles between 1990 and 2020 met the inclusion criteria and were analyzed. Six unpublished cases of cutaneous FeL managed by Italian dermatologists were also reviewed. The majority of cases were reported from South America, followed by Europe and North America. Nodules were the most frequently reported clinical signs and the presence of Leishmania in lesioned skin was assessed mainly by cytology. A total of six Leishmania species have been identified as being responsible for skin lesions. Coinfections by FIV or FeLV were reported in 12.1% and 9.1% of the cases, respectively. Clinical data including treatment have been analyzed and discussed to provide directives for proper management of the disease for which cats may also serve as domestic reservoirs for human infections.
- Published
- 2021
- Full Text
- View/download PDF
144. Disappearance of signs of heat and induction of ovulation in oestrous queens with gonadorelin: a clinical study.
- Author
-
Ferré-Dolcet L, Frumento P, Abramo F, and Romagnoli S
- Subjects
- Animals, Cats, Female, Gonadotropin-Releasing Hormone, Ovariectomy veterinary, Ovary, Hot Temperature, Ovulation
- Abstract
Objectives: The objective of this study was to assess the efficacy of a single intramuscular administration of gonadorelin to induce ovulation in queens in oestrus., Methods: Twenty-seven queens presented in oestrus for elective ovariectomy were divided into a treatment (n = 19) and a placebo (n = 8) group. Treated queens received a 50 µg dose of gonadorelin, while placebo-treated queens were injected intramuscularly (IM) with an equal amount of saline solution. All treatments were performed between the second and fourth days of heat., Results: Two days later, signs of behavioural heat had disappeared in all gonadorelin-treated queens, while 5/8 placebo-treated queens were still in heat. Following ovariectomy, performed 4 days after drug administration, the ovaries of each queen were evaluated histologically and the number of corpora lutea were counted. Sixteen of 19 (84%) gonadorelin-treated queens had ovulated and developed five (range 2-9) corpora lutea, while 3/8 (37%) placebo-treated queens had ovulated and developed five (range 3-6) corpora lutea., Conclusions and Relevance: This is the first study to document the efficacy of a 50 µg/cat gonadorelin dose to induce ovulation in oestrous queens when administered IM on days 2-4 following the onset of oestrus.
- Published
- 2021
- Full Text
- View/download PDF
145. Histopathological Classification of Canine Cutaneous Round Cell Tumors Using Deep Learning: A Multi-Center Study.
- Author
-
Salvi M, Molinari F, Iussich S, Muscatello LV, Pazzini L, Benali S, Banco B, Abramo F, De Maria R, and Aresu L
- Abstract
Canine cutaneous round cell tumors (RCT) represent one of the routine diagnostic challenges for veterinary pathologists. Computer-aided approaches are developed to overcome these restrictions and to increase accuracy and consistency of diagnosis. These systems are also of high benefit reducing errors when a large number of cases are screened daily. In this study we describe ARCTA (Automated Round Cell Tumors Assessment), a fully automated algorithm for cutaneous RCT classification and mast cell tumors grading in canine histopathological images. ARCTA employs a deep learning strategy and was developed on 416 RCT images and 213 mast cell tumors images. In the test set, our algorithm exhibited an excellent classification performance in both RCT classification (accuracy: 91.66%) and mast cell tumors grading (accuracy: 100%). Misdiagnoses were encountered for histiocytomas in the train set and for melanomas in the test set. For mast cell tumors the reduction of a grade was observed in the train set, but not in the test set. To the best of our knowledge, the proposed model is the first fully automated algorithm in histological images specifically developed for veterinary medicine. Being very fast (average computational time 2.63 s), this algorithm paves the way for an automated and effective evaluation of canine tumors., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Salvi, Molinari, Iussich, Muscatello, Pazzini, Benali, Banco, Abramo, De Maria and Aresu.)
- Published
- 2021
- Full Text
- View/download PDF
146. Evaluation of duodenal endoscopic and histologic findings, including counts of forkhead box P3-positive regulatory T cells, in dogs with immunosuppressant-responsive enteropathy.
- Author
-
Benvenuti E, Pierini A, Benali SL, Gori E, Abramo F, Bottero E, Pietra M, Ruggiero P, and Marchetti V
- Subjects
- Animals, Dogs, Duodenum, Immunosuppressive Agents therapeutic use, T-Lymphocytes, Regulatory, Dog Diseases, Inflammatory Bowel Diseases veterinary
- Abstract
Objective: To prospectively evaluate the clinical and prognostic importance of duodenal endoscopic and histologic findings, including duodenal mucosal counts of forkhead box P3-positive regulatory T cells (Foxp3+ Tregs), in dogs with immunosuppressant-responsive enteropathy (IRE)., Animals: 57 client-owned dogs with IRE., Procedures: The canine chronic enteropathy clinical activity index (CCECAI) was used to assess each dog when IRE was diagnosed (T0) and 1, 3, 6, and 12 months later. Dogs were grouped on the basis of clinical response (responder group vs nonresponder group) and 12-month long-term outcome (responded to treatment and did not relapse [good outcome group] vs did not respond to treatment or had relapsed [bad outcome group]). At T0, dogs underwent gastrointestinal endoscopy and endoscopic biopsy, with results for variables of duodenal endoscopic and histologic evaluations scored and compared across groups., Results: At T0, the overall median CCECAI score was 7; CCECAI score was not associated with clinical response or relapse. Dogs had significantly greater odds of being in the bad outcome group (vs the good outcome group) if they had a histologic score of 3 (OR, 3.5; 95% CI, 1.09 to 11.3). No differences in the counts of Foxp3+ Tregs were detected between groups., Conclusions and Clinical Relevance: In dogs with IRE, results indicated that evaluation of Foxp3+ Tregs did not have prognostic value, whereas a duodenal histologic score of 3 could be a negative prognostic factor for response and relapse, and higher severity scores for intraepithelial lymphocytes and lamina propria lymphocytes and plasma cells in duodenal biopsy samples may be negatively associated with response.
- Published
- 2021
- Full Text
- View/download PDF
147. Transmammary transmission of Troglostrongylus brevior feline lungworm: a lesson from our gardens.
- Author
-
Bezerra-Santos MA, Mendoza-Roldan JA, Abramo F, Lia RP, Tarallo VD, Salant H, Brianti E, Baneth G, and Otranto D
- Subjects
- Animals, Cats, Female, Male, Respiratory Tract Infections parasitology, Respiratory Tract Infections pathology, Respiratory Tract Infections transmission, Strongylida Infections parasitology, Strongylida Infections transmission, Cat Diseases parasitology, Cat Diseases transmission, Infectious Disease Transmission, Vertical veterinary, Respiratory Tract Infections veterinary, Strongylida, Strongylida Infections veterinary
- Abstract
Feline lungworms such as Aerulostrongylus abstrusus and Troglostrongylus brevior are snail-borne pathogens causing respiratory disease in domestic cats. Paratenic hosts such as rodents and reptiles have also been implicated in the epidemiology of these parasites. Although A. abstrusus has been recognized for a long time as the most prevalent lungworm among cats worldwide, T. brevior is of major concern in kittens. Bearing in mind that disease due to T. brevior occurs mainly in pediatric patients younger than 6 months of age, the diagnosis of this parasite in two kittens presenting severe respiratory disease from the garden of one of the authors inspired us to investigate the potential routes of transmission for T. brevior in domestic cats. Of the three queens (A, B and C) that delivered kittens (n = 8), only cat A was positive for T. brevior, presenting her two kittens severe respiratory clinical signs, which lead to the exitus in one of them, 18 days of age. In addition, three kittens, the offspring of queen B, turned to be positive at the coprological examination after suckling from queen A, whereas those from queen C (that suckled only on their own mother) remained negative. A series of coprological, histological and molecular tests were conducted to confirm the presence of T. brevior in the patients as well as in the other cats cohabiting the same garden. Adult nematodes were retrieved from the trachea and bronchi of the dead kitten (kitten 1A), and larvae at the histology of the lung and liver parenchyma associated with bronco pneumonitis and lymphocytic pericholangitis, respectively. Cornu aspersum (n = 60), Eobania vermiculata (n = 30) snails (intermediate hosts) as well as lizards and rats (potential paratenic hosts) were collected from the same garden and processed through tissue digestion and molecular detection. Troglostrongylus brevior larvae were recovered through tissue digestion from two C. aspersum (3.33 %) and it was confirmed by PCR-sequencing approach, which also detected T. brevior DNA in the liver and lungs of one rat and in the coelomatic cavity of one gecko lizard. During the COVID-19 lockdown, when scientists spent more time at home, we grasp the opportunity to decipher T. brevior biology and ecology starting in a small ecological niche, such as the garden of our house. Data herein presented led us to suggest: i) the transmammary transmission of T. brevior in domestic cats; ii) the role of intermediate and paratenic hosts (including reptiles) in the epidemiology of the infection which they transmit; as well as iii) the importance of observational parasitology in studying any event that certainly occurs in small ecological niches, as it could be in our home gardens., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
148. Age-related changes of seminiferous tubule morphology, interstitial fibrosis and spermatogenesis in dogs.
- Author
-
Tesi M, Lazzarini G, Magliaro C, Abramo F, Fanelli D, Miragliotta V, and Rota A
- Subjects
- Age Factors, Animals, Cell Shape, Dog Diseases pathology, Fibrosis pathology, Fibrosis veterinary, Male, Seminiferous Epithelium pathology, Seminiferous Epithelium ultrastructure, Seminiferous Tubules pathology, Seminiferous Tubules ultrastructure, Sexual Maturation physiology, Aging physiology, Dogs, Seminiferous Tubules cytology, Spermatogenesis physiology, Testicular Diseases pathology
- Abstract
There are age-related changes in testicular anatomy and physiology whereby there are modifications of sperm production and reproductive hormone functions. Effects of age on testicular microanatomy are well documented in humans, while there is limited understanding of these changes in dogs. The aim of this study was to evaluate age-related changes of seminiferous tubule morphology, interstitial fibrosis and spermatogenesis in dogs. Dogs (n = 32) were divided into four age groups: peripubertal (n = eight), relatively younger (n = seven), reproductively mature (n = seven) and relatively older (n = ten). Picrosirius Red stained sections were used for morphometrical analysis of testicular tissues, while the characteristics of seminiferous epithelium were assessed using a modified Johnsen scoring system for haematoxylin and eosin stained sections. Seminiferous epithelium and seminiferous tubule area increased from peripuberty to reproductive maturity, indicating there were changes during sexual maturation and subsequently there were decreases with further aging. There was a similar age-related trend for changes in seminiferous epithelium height with values being greatest in reproductively mature dogs; while there were no age-related differences in tubular diameter. Collagen content in the testicular interstitium gradually decreased from peripuberty to the age when dogs were reproductively mature and there were subsequent increases in relatively older dogs, thus, there was an association between the extent of testicular fibrosis and senescence. There was a decrease in spermatogenetic functions from relatively younger to older ages. Further investigations are warranted to establish mechanisms responsible for age-related changes of testicular morphology and related clinical implications., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper, (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
149. Acral congenital superficial dermal lymphatic malformations in two unrelated cats: clinicopathological, dermoscopic and ultrastructural findings.
- Author
-
Legnani S, Abramo F, Zanna G, Graziano L, Cornegliani L, and Roccabianca P
- Subjects
- Animals, Biopsy, Cats, Male, Skin pathology, Vascular Malformations pathology, Cat Diseases pathology, Dermoscopy veterinary, Lymphoid Tissue pathology, Vascular Malformations veterinary
- Abstract
Background: Cutaneous vascular malformations (CVM) represent a spectrum of human diseases identified at birth or in paediatric patients and classified according to the type of vessel affected. Confusing classification in human medicine has led to misdiagnoses and frequent nomenclature revision. Cutaneous lymphatic malformations (CLM) are reported sporadically in humans., Objective: To describe the clinicopathological findings of superficial dermal cutaneous lymphatic malformations (SDCLM) in two cats., Animals: Two unrelated adult domestic short hair cats., Methods: The two cats were evaluated clinically and with a dermoscope for recurrent swelling and presence of vesicles oozing serosanguineous fluid affecting the right and left hind foot, respectively, since birth. Skin biopsy specimens were collected for histopathological and immunohistochemical evaluation and electron microscopy., Results: A CVM was suspected based on the age of onset, clinical signs, results of diagnostic imaging and histopathological findings. Dermoscopy was used to describe the alterations of the skin surface. The involvement of the lymphatic vessels was confirmed using immunohistochemical findings and electron microscopy., Conclusion and Clinical Importance: To the best of the authors' knowledge, this is the first description of the clinical, dermoscopic, histopathological and ultrastructural characteristics of SDCLM in cats resembling the human counterpart. SDCLMs are rare conditions and appropriate histopathological and immunohistochemical confirmation is required to avoid misdiagnosis and mistreatment., (© 2020 ESVD and ACVD.)
- Published
- 2020
- Full Text
- View/download PDF
150. Clinical, haematological and biochemical findings in tigers infected by Leishmania infantum.
- Author
-
Cavalera MA, Iatta R, Laricchiuta P, Passantino G, Abramo F, Mendoza-Roldan JA, Otranto D, and Zatelli A
- Subjects
- Animals, Animals, Zoo parasitology, Blood Proteins analysis, Endangered Species, Hypergammaglobulinemia parasitology, Hypergammaglobulinemia veterinary, Italy epidemiology, Leishmaniasis, Visceral blood, Leishmaniasis, Visceral diagnosis, Leishmaniasis, Visceral epidemiology, Leishmania infantum isolation & purification, Leishmaniasis, Visceral veterinary, Tigers parasitology
- Abstract
Background: A large number of animal species are susceptible to Leishmania infantum (Kinetoplastida, Trypanosomatidae) in endemic areas, including domestic and wild felids such as tigers (Panthera tigris). Knowledge on the infection of this endangered species is still at its infancy, and therefore this study aims to identify clinical presentation and clinicopathological findings of tigers naturally infected by L. infantum., Results: Tigers either L. infantum-positive (group A) or -negative (group B) were apparently healthy or presented visceral leishmaniasis unrelated conditions, except for one animal in which a large non-healing cutaneous lesion was observed. However, histological exam and immunohistochemistry carried out on the lesion excluded the presence of L. infantum amastigotes. Biochemical analysis showed that the average concentration of total proteins, globulins and haptoglobin were significantly higher (p < 0.01, p = 0.01 and p = 0.02, respectively), while the albumin/globulin ratio significantly lower (p = 0.05) in group A compared with group B. The biochemical alterations were partially confirmed by the serum protein electrophoresis results revealing a significant increase in the total protein value (p = 0.01) and hypergammaglobulinemia (p = 0.03) but an unmodified albumin/globulin ratio in group A., Conclusions: In this study tigers infected by L. infantum have shown to be mainly asymptomatic. The absence of clinical signs may lead veterinarians to overlook leishmaniasis in animals kept in captivity. Therefore, diagnostic and screening tests as serology should be part of routinely surveillance programs to be performed on tigers in zoological gardens located in endemic areas. Though only few protein-related laboratory abnormalities were recorded in infected animals, they could provide diagnostic clues for a first suspicion of L. infantum infection in tigers. Indeed, considering the high risk of zoonotic transmission in heavily frequented environment as zoos, a prompt diagnosis of L. infantum infection is of pivotal importance.
- Published
- 2020
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.