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Two types of lung cells (epithelial cancer lung cells, A-549 and lung fibroblasts MRC-5) were exposed to the clinically established gold drug auranofin at concentrations close to the half-maximal inhibitory drug concentrations (IC50). Collected cells were subjected to speciation analysis using inductively coupled plasma mass spectrometry (ICP-MS). Auranofin showed better affinity toward proteins than DNA, RNA, and hydrophilic small molecular weight compounds. It can bind to proteins that vary in size (~20 kDa, ~75 kDa, and ≥200 kDa) and pI. However, the possibility of dimerization and protein–protein complex formation should also be taken into account. µRPLC/CZE-ESI-MS/MS studies on trypsinized proteins allowed the indication of 76 peptides for which signal intensity was influenced by auranofin presence in cells. Based on it, identity was proposed for 20 proteins. Except for thioredoxin reductase (TrxR), which is directly targeted by gold complex, the proteins were found to be transformed. Five indicated proteins: myosin, plectin, talin, two annexins, and kinase M3K5, are responsible for cell–cell, cell–protein interactions, and cell motility. A wound healing test confirmed their regulation by auranofin as cell migration decreased by 40% while the cell cycle was not interrupted.

Graphene-based nanocomposites constitute an interesting and promising material for various applications. Intensive progress in the development of this group of materials offers an opportunity to create new systems useful for drinking water decontamination or other biotechnological applications. Nanohybrid structures of graphene-ceramic systems can be obtained using covalent graphene surface modification with nanoparticles (NPs) of ceramic and/or co-deposition of metals with selected morphology and chemistry. The present paper systematizes the associated bio-related knowledge and inspires future development of graphene/NPs systems. Emerging knowledge and unique research techniques are reviewed within designing the required nanocomposite structure and chemical composition, development and optimization of new methods of covalent surface modification of graphene with NPs as well as analysis of mechanisms governing the formation of covalent bonding. Further, innovative research tools and methodologies are presented regarding the adjustment of functionalities of materials used for the application in drinking water decontamination or biocidal composites. This study provides a comprehensive base for rational development of more complex, hybrid graphene-based nanomaterials with various bio-functionalities that can be further applied in industrial practice.

Purpose: The beginning work in a new place for an employee means a lot of stress resulting from confrontation with unfamiliar surroundings and people. The process of introducing an employee to work should involve not only the employer, but also the entire team, emphasizing care, willingness to help and support at such an important initial stage. The main aim of the research is to examine whether the adaptation of new employees in a trade enterprise is effective. An attempt has been made to identify the conditions in the process of social and professional adaptation of a new employee through the prism of corporate social responsibility. Design/methodology/approach: The paper is consisted with 2 parts. The first is about a theoretical context of CSR in small trade company level and personnel management issues and the adaptation of a new employe. The second part includes findings on research. The case study was used to develop research problem. The research questions included the issue of stress in the adaptation process and the role of the introducer. The study covered 100% of the study population. Data were analyzed using descriptive and non-parametric statistics methods (e.g. classification trees method to identify the importance of predictors). Findings: The research results indicate that the adaptation of a new employee is an important element of his/her quality of professional life, and for the organization it is an important aspect of implementing the social responsibility strategy. Taking into account the needs of employees, as internal stakeholders of the company, is an expression of the implementation of socially responsible management. The implementation of the CSR strategy can be assessed by the degree of employee satisfaction with the quality of work life, which results from the effectiveness of fulfilling tasks in the adaptation process. Research limitations/implications: Due to the fact that research is limited to the one chosen company results can not be uncritically generalized. This study covers only a narrow part from a wide range of problems in shaping social responsibility in business. It only applies to actions taken towards employees in the adaptation process. Due to this implication, research should be deepened and expanded to include additional aspects both in terms of responsibility towards employees and other stakeholder groups. Practical implications: The research results can be a source of information for managers about factors important in shaping the personnel function of a socially responsible organization, primarily in the field of social and professional adaptation. Social implications: The article contributes to the development of knowledge and dissemination of good practices in the field of corporate social responsibility. Originality/value: The article presents a new approach to managing the personnel function in a business organization through the prism of the principles of social responsibility. Previous research does not directly or comprehensively take into account possible connections between corporate social responsibility and the adaptation process. [ABSTRACT FROM AUTHOR]

Contemporary tasks and imposed requirements in the area of education are focused on improving the efficiency and quality of teaching, including counteracting the disturbing phenomena of the “day of liquid modernity” [1, p. 15–29]. The teacher's person is perceived subjectively, from the perspective of the contractor of professional assumptions. The dynamics of educational processes and progressive civilization changes forces us to meet the emerging expectations of educational policy. The look of many leading educators, among others Jan Władysław Dawid, Zygmunt Mysłakowski, Wincenty Okoń, Maria Grzegorzewska, Czesław Banach for personality traits of the teacher has gone to oblivion to give way to schematic and mass teaching. Increasingly, attention is paid to how? and not who? teaches. Studies in the field of psychology and pedagogy pose a question; What should the teacher be like? Using the following article, in which I will base myself on empirical research, I want to answer a completely different aspect, what kind of teacher would the students want?

Kidney transplantation from a donor with latent BKPyV might be the cause of serious complications, such as BK virus-associated nephropathy. The aim of the study was to determine the prevalence of BKPyV infection in donors after brain death (DBDs), to analyse the molecular variation of BKPyV and to compare clinical and inflammation parameters of DBDs infected with various genotypes of BKPyV. BKPyV was investigated in blood and urine samples of 103 DBDs using PCR followed by sequencing and bioinformatic analysis, and the viral load was assessed by qPCR. Clinical parameters, including cellular markers of inflammation were assessed. The results confirm high prevalence of BKPyV (48%),and genotype IV (49%) over genotype I (43%) and the co-infection with genotypes I and IV in 8.2%. Viral load ranged from 102 to 107 copies/mL, with an average of 1.92 × 106 copies/mL. No specific markers for BKPyV infection were detected among the parameters tested. Infection with genotype I may be associated with the adverse impact on thekidney function, while infection with genotype IV was associated with the anemia Not only the viral load but also the genotype of BKPyV may have an impact on the course of infection.

Two-dimensional compounds with nanostructural features are attracting attention from researchers worldwide. Their multitude of applications in various fields and vast potential for future technology advancements are successively increasing the research progress. Wastewater treatment and preventing dangerous substances from entering the environment have become important aspects due to the increasing environmental awareness, and increasing consumer demands have resulted in the appearance of new, often nonbiodegradable compounds. In this review, we focus on using the most promising 2D materials, such as MXenes, Bi2WO6, and MOFs, as catalysts in the modification of the Fenton process to degrade nonbiodegradable compounds. We analyze the efficiency of the process, its toxicity, previous environmental applications, and the stability and reusability of the catalyst. We also discuss the catalyst’s mechanisms of action. Collectively, this work provides insight into the possibility of implementing 2D material-based catalysts for industrial and urban wastewater treatment.

The combination of fibre metal laminates (FML) and sandwich structures can significantly increase the performance under impact of FMLs. The goal of this work was to create a material that will combine the superior properties of FMLs and foam sandwich structures in terms of the impact resistance and simultaneously have lower density and fewer disadvantages related to the manufacturing. An extensive impact testing campaign has been done using conventional fibre metal laminates (carbon- and glass-based) and in the proposed fibre foam metal laminates to assess and compare their behaviour. The main difference was observed in the energy absorption mechanisms. The dominant failure mechanism for fibre foam laminates is the formation of delaminations and matrix cracks while in the conventional fibre metal laminate the main failure mode is fibre cracking due to high local stress concentrations. The reduction in the fibre cracking leads to a better after-impact resistance of this type of structure improving the safety of the structures manufactured with these materials.

The BK polyomavirus (BKPyV), a representative of the family Polyomaviridae, is widespread in the human population. While the virus does not cause significant clinical symptoms in immunocompetent individuals, it is activated in cases of immune deficiency, both pharmacological and pathological. Infection with the BKPyV is of particular importance in recipients of kidney transplants or HSC transplantation, in which it can lead to the loss of the transplanted kidney or to haemorrhagic cystitis, respectively. Four main genotypes of the virus are distinguished on the basis of molecular differentiation. The most common genotype worldwide is genotype I, with a frequency of about 80%, followed by genotype IV (about 15%), while genotypes II and III are isolated only sporadically. The distribution of the molecular variants of the virus is associated with the region of origin. BKPyV subtype Ia is most common in Africa, Ib-1 in Southeast Asia, and Ib-2 in Europe, while Ic is the most common variant in Northeast Asia. The development of molecular methods has enabled significant improvement not only in BKPyV diagnostics, but in monitoring the effectiveness of treatment as well. Amplification of viral DNA from urine by PCR (Polymerase Chain Reaction) and qPCR Quantitative Polymerase Chain Reaction) is a non-invasive method that can be used to confirm the presence of the genetic material of the virus and to determine the viral load. Sequencing techniques together with bioinformatics tools and databases can be used to determine variants of the virus, analyse their circulation in populations, identify relationships between them, and investigate the directions of evolution of the virus.

Seeing of emptiness and mystical experience — the case of Madhyamaka: The problem of Buddhist religiosity is one of the most classic problems of Buddhist studies. A particular version of this issue is the search for mystical experience in Buddhism. This is due to the conviction that mystical experience is the essence of religious experience itself. The discovery of such an alleged experience fuels comparative speculations between Buddhism and the philosophical and religious traditions of the Mediterranean area. Madhyamaka is the Buddhist tradition which many researchers saw as the fulfillment of such mystical aspirations in Buddhism. In this paper I specify the standard parameters of mystical experience (non‑conceptuality, ineffability, paradoxicality, silence, oneness, fullness) and I conclude that they either cannot be applied to Madhyamaka or that the application is only illusory.